Beckman Coulter ACT - Service Manual PDF

BECKMAN COULTER™ AC•T™ 5diff
Hematology Analyzer
Service Manual
PN 4237616B (September 2000)

COULTER CORPORATION
A Beckman Coulter Company
Miami, Florida 33196-2500 USA
LEGAL NOTICES
Beckman Coulter, Inc. makes no representation that, upon furnishing this service manual, the holder of the manual will have
the necessary technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the
manual. Beckman Coulter, Inc. assumes no liability whatsoever, including consequential and incidental damages, resulting
from improper operation of Beckman Coulter instruments after maintenance of Beckman Coulter instruments has been
performed by persons not employed by Beckman Coulter, Inc. Furthermore, Beckman Coulter, Inc. assumes no liability
whatsoever for any personal injury or property damage resulting from maintenance and/or repair of Beckman Coulter
instruments performed by persons not employed by Beckman Coulter, Inc.
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL
BEFORE ATTEMPTING TO OPERATE INSTRUMENT.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Might cause injury.

CAUTION - Might cause damage to the instrument.
IMPORTANT - Might cause misleading results.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards such as the use of barrier
protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when
operating or maintaining this or any other automated laboratory analyzer.
"This Service Manual contains confidential information of Beckman Coulter, Inc. and its receipt or possession does not
convey any rights to reproduce, disclose its contents, or to manufacture, use, or sell anything it may describe.
Reproduction, disclosure, or use without specific written authorization of Beckman Coulter, Inc. is strictly forbidden."
Copyright © Beckman Coulter, Inc. 1999-2000

All rights reserved.
REVISION STATUS
Initial Issue, 3/2000

Released by CN 040130-0003
Software Version 0.11
Issue B, 07/00
Released by CN 040150-0025
Software version 1.03
The material in the revision B change pages was updated for software version 1.03 and for any hardware
changes since revision A. The changes include updating the adjustment procedures for the bath assembly,
HGB blank, RBC/PLT gain, WBC/BASO, motor current, thresholds, and the optical bench; updating the
replacement procedures for the heater assembly, power supply, start switch, reagent syringes, count
syringe, sample prove, waste syringe, 5diff syringe, flow-cell coax, optical bench lamp, diluent reservoir,
and sample syringe; updating the procedure for testing and configuring the bar-code reader; updating the
parts lists; updating the tubing lists and associated circuit connections; adding procedures for balancing
the WBC count, setting the diff+/diff- thresholds, and replacing the new Main card; and adding
information on the LX300 + printer.
Changes were made on the following pages:

1.1-1, 1.1-2, 1.1-4
2.1-4, 2.6-1, 2.6-2, 2.6-3, 2.6-4, 2.6-5, 2.8-3, 2.8-11, 2.8-12, 2.8-13, 2.8-15, 2.10-2, 2.10-3
3.1-1, 3.2-1, 3.2-2, 3.2-3, 3.2-6, 3.2-7, 3.2-9, 3.2-10, 3.3-1, 3.3-7, 3.3-8
added 3.3-9 and 3.3-10
4.1-1, 4.1-2, 4.1-3, 4.1-4, 4.2-1, 4.2-2, 4.2-4, 4.2-5, 4.2-6 through 4.2-8, 4.4-1, 4.5-1 through 4.5-4
4.6-1 through 4.6-8, 4.7-2 4.8-1, 4.9-1, 4.9-2, 4.10-1, 4.10-2, 4.11-1, 4.12-1, 4.12-2, 4.13-1; 4.13-2
4.14-1, 4.14-2, 4.17-2, 4.17-3, 4.18-1 4.19-1 through 4.19-8, 4.20-1, 4.20-2, 4.20-3, 4.20-4
deleted 4.20-5 and 4.20-6
4.21-1 through 4.21-3, 4.23-1 through 4.23-6, 4.24-1 through 4.24-6, 4.25-1 through 4.25-8
deleted 4.25-9 through 4.25-12
4.26-1, 4.26-3, 4.26-4, 4.27-1 through 4.27-8
deleted 4.27-9 through 4.27-12
4.29-1, 4.29-3 through 4.29-6, 4.30-1, 4.30-3 through 4.30-5, 4.31-1, 4.31-3, 4.31-4
4.32-1 through 4.32-3, 4.33-1, 4.33-2, 4.35-1, 4.35-2, 4.35-3, 4.35-10, 4.36-1, 4.36-2, 4.36-5, 4.36-6
added 4.37-1 and 4.37-2, 4.38-1 and 4.38-2, 4.39-1 through 4.39-10
5.2-3, 6.3-2 through 6.3-6, 7.3-2
8.1-1 through 8.1-12, 8.2-7, 8.2-8, 8.2-9, 8.2-16, 8.2-17, 8.2-42, 8.2-46, 8.2-48
A.2-9, A.3-3, A.4-1, C.1-1, C.1-2 and C.1-7.
The change page packet also includes the latest revision of the Pneumatic/Hyraulic Schematic, 7616069B.
Changes that are part of the most recent revision are indicated in the printed copy by a bar in the margin of
the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, the changes will be included on minor revision change pages or summarized on a Notice of
Information Update form and will be released by service memo.
PN 4237616B iii
REVISION STATUS
iv PN 4237616B
CONTENTS
LEGAL NOTICES, 2
REVISION STATUS, iii
CONTENTS, v
1 INTRODUCTION, 1.1-1
1.1 MANUAL DESCRIPTION, 1.1-1

Scope, 1.1-1
Notification of Updates, 1.1-1
Intended Audience, 1.1-1
Organization, 1.1-1
Numbering Format, 1.1-2
Special Headings, 1.1-3
WARNING, 1.1-3
CAUTION, 1.1-3
IMPORTANT, 1.1-3
ATTENTION, 1.1-3
Note, 1.1-3
Conventions, 1.1-3
Graphics, 1.1-4
1.2 SAFETY PRECAUTIONS, 1.2-1

Electronic, 1.2-1
Biological, 1.2-1
Troubleshooting, 1.2-1
2 INSTRUMENT DESCRIPTION, 2.1-1
2.1 INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER, 2.1-1

Purpose, 2.1-1
Function, 2.1-1
Description, 2.1-1
Components, 2.1-1
Interaction with the AC•T 5diff Hematology Analyzer, 2.1-1
Modes of Operation, 2.1-3
CBC Mode, 2.1-3
CBC/DIFF Mode, 2.1-3
Reagent Consumption, 2.1-4
2.2 OPERATION PRINCIPLES, 2.2-1

Overview, 2.2-1
Measurement Principles, 2.2-1
Coulter Principle, 2.2-1
Aperture Sensor System, 2.2-1
Applying the Coulter Principle, 2.2-2
ACV Technology, 2.2-3
Dual Focused Flow (DFF), 2.2-3
Flow Cell, 2.2-3
Focused Flow Impedance, 2.2-4
Absorbance Cytochemistry, 2.2-4
PN 4237616B v
CONTENTS
Signal Processing, 2.2-4

Thresholds, 2.2-4
WBC/BASO Methodology, 2.2-5
Sample Analysis Overview, 2.2-5
Aspiration, 2.2-5
Dilution, 2.2-6
Delivery, 2.2-6
Sample Partitioning, 2.2-7
2.3 CYCLE DESCRIPTION, 2.3-1

Cycle Start Conditions, 2.3-1
Sample Flow, 2.3-2
2.4 SAMPLE ANALYSIS, 2.4-1

RBC and Platelet Analysis, 2.4-1
Parameter Results Obtained from the RBC/Plt Dilution, 2.4-2
Hgb Measurement, 2.4-3
WBC Count and Differential, 2.4-4
Parameter Results Obtained from the WBC/BASO Dilution, 2.4-5
Differential, 2.4-5
Parameter Results Obtained from the DIFF Dilution, 2.4-6
Dilution Summary, 2.4-7
2.5 RBC PARAMETER DEVELOPMENT, 2.5-1

RBC/Plt Dilution, 2.5-1
RBC Count, 2.5-1
RBC Histogram, 2.5-1
Parameter Results Obtained Using the RBC Histogram, 2.5-2
Hct Measurement, 2.5-2
MCV Calculation, 2.5-2
RDW Calculation, 2.5-2
RBC Distribution Flags, 2.5-2
RBC1 and RBC2 Thresholds, 2.5-3
Flags, 2.5-3
Hgb Determination, 2.5-3
Hgb Blank Reading, 2.5-3
Sample Reading, 2.5-4
Hgb Specific Flags, 2.5-4
MCH and MCHC Calculations, 2.5-4
MCH Calculation, 2.5-4
MCHC Calculation, 2.5-4
2.6 PLATELET PARAMETER DEVELOPMENT, 2.6-1

Plt Count, 2.6-1
Platelet Distribution Curve, 2.6-1
Parameter Results Obtained Using the Plt Histogram, 2.6-2
MPV Measurement, 2.6-2
Pct Calculation, 2.6-2
PDW Calculation, 2.6-2
vi PN 4237616B
CONTENTS
Detecting Abnormal Platelet Distributions, 2.6-3

Identifying a Normal Distribution, 2.6-3
Interference on the Lower End of the Platelet Distribution Curve, 2.6-3
Microcytic Interferences on the Upper End of the Platelet Distribution
Curve, 2.6-3
Microcytic Interference with a Distinct Valley between 18 fL and 25 fL, 2.6-4
Microcytic Interference with a Valley below 18 fL, 2.6-4
Interference with No Distinct Valley, 2.6-5
2.7 WBC PARAMETER DEVELOPMENT, 2.7-1

Overview, 2.7-1
WBC/BASO Dilution, 2.7-1
WBC Count, 2.7-1
BASO Count, 2.7-1
DIFF Dilution, 2.7-2
DiffPlot Development, 2.7-2
DiffPlot Regions Defined, 2.7-3
Neutrophil (Neut), 2.7-3
Lymphocyte (Lymph), 2.7-3
Monocyte (Mono), 2.7-3
Eosinophil (Eos), 2.7-3
Debris, 2.7-3
Immature White Blood Cells, 2.7-4
Immature Granulocytes, 2.7-4
Band Cells, 2.7-4
Blast Cells, 2.7-4
DiffPlot Thresholds, 2.7-4
2.8 PNEUMATIC/HYDRAULIC SYSTEM, 2.8-1

Functions of Valves, 2.8-1
Pneumatic Diagrams, 2.8-4
Diluter System, 2.8-11
Diluent Input (Figure 2.8-10), 2.8-11
5diff Syringe and Flow Cell, 2.8-12
Probe and Probe Rinse, 2.8-13
Diluent to Baths, 2.8-13
Waste System, 2.8-15
2.9 ELECTRONIC SYSTEM, 2.9-1

Plug/Jack Labels, 2.9-1
Optical Preamplifier Card, 2.9-1
LCD and Keypad Card, 2.9-1
LED Card, 2.9-1
Motor Interconnect Card, 2.9-1
Traverse Interconnect Card, 2.9-1
2.10 SOFTWARE STRUCTURE, 2.10-1

Overview, 2.10-1
Menu Trees, 2.10-1
How to Select a Menu Item, 2.10-1
PN 4237616B vii
CONTENTS
3 INSTALLATION PROCEDURES, 3.1-1
3.1 PREINSTALLATION CHECKS, 3.1-1

Environment, 3.1-1
Altitude Range, 3.1-1
Ambient Temperature, 3.1-1
Space and Accessibility Requirements, 3.1-1
Electrical Input, 3.1-1
Power Requirements, 3.1-2
Grounding, 3.1-2
Installation Category, 3.1-2
Electromagnetic Environment Check, 3.1-2
Inspection Report, 3.1-2
3.2 INITIAL SETUP, 3.2-1

Preinstallation Checks, 3.2-1
Supplies, 3.2-1
Unpacking, 3.2-1
Inspection, 3.2-1
Unpack the Analyzer, 3.2-1
Unpack the Installation Kit, PN XEA484A, 3.2-1
Unpack the Waste Alarm Kit, PN 6912680, 3.2-1
Verify All Caution and Compliance Labels are in Place, 3.2-1
Connect the Waste System, 3.2-3
Connect the Waste Tubing, 3.2-3
Install the Waste Alarm, 3.2-3
Connect the Reagents, 3.2-5
Connect the Diluent Tubing, 3.2-5
Install the Reagent Bottles, 3.2-6
Install the Printer, 3.2-7
Power On the Instrument, 3.2-7
Enter Reagent Lot Numbers, 3.2-8
Prime the Instrument, 3.2-8
Configure the Instrument Printer Settings, 3.2-9
Set the User Mode, 3.2-9
Verification, 3.2-10
3.3 PRINTER INSTALLATION, 3.3-1

EPSON® LX™- 300 and LX™- 300+ Printer Connection, 3.3-1
Unpack the Printer, 3.3-1
Install the Knob, 3.3-1
Install the Ribbon Cartridge, 3.3-2
Connect the Printer, 3.3-3
Paper Feed Options, 3.3-4
Single Sheet Paper Feed Setup, 3.3-4
Loading Continuous Feed Paper Feed, 3.3-5
Configure the Printer, 3.3-7
LX300 Printer, 3.3-7
LX300+ Printer, 3.3-7
Complete the Instrument Installation, 3.3-9
viii PN 4237616B
CONTENTS
4 SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1 GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY

ANALYZER, 4.1-1
General Guidelines, 4.1-1
Safety Precautions, 4.1-1
Accessibility, 4.1-1
Electronic Precautions, 4.1-1
Environment Protection, 4.1-1
Procedures, 4.1-1
Tools and Supplies, 4.1-2
Instrument Performance Verification, 4.1-2
Service Password, 4.1-2
User Mode, 4.1-2
How to Disable the Right Side Door Interlock, 4.1-2
How to Reactivate the Right Side Door Interlock, 4.1-3
Power Down / Power Up the Instrument, 4.1-3
Purpose, 4.1-3
Power Down, 4.1-3
Power Up, 4.1-4
Reset the Instrument, 4.1-4
System Reset Cycle, 4.1-4
Hardware Reset, 4.1-4
4.2 OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND

COVERS, 4.2-1
Purpose, 4.2-1
Tools/Supplies Needed, 4.2-1
Opening the Right Side Door, 4.2-1
Bypassing the Right Side Door interlock, 4.2-1
Removing the Left Side Panel, 4.2-2
Removal, 4.2-2
Opening the Main Card Door, 4.2-3
Installation, 4.2-3
Removing the Rear Access Panel, 4.2-3
Removal, 4.2-4
Installation, 4.2-4
Removing the Top Cover, 4.2-4
Removal, 4.2-4
Installation, 4.2-5
Removing the Front Cover, 4.2-5
Removal, 4.2-5
Installation, 4.2-7
4.3 PREPARATION TO SHIP THE INSTRUMENT, 4.3-1

Purpose, 4.3-1
Bleach the Baths (20 minutes), 4.3-1
Clean the External Surfaces (20 minutes), 4.3-2
Clean the Tubing and Chambers (60 minutes), 4.3-2
PN 4237616B ix
CONTENTS
Preparation, 4.3-2
Cycle Routine, 4.3-3
Drain and Rinse, 4.3-3
4.4 FLOW CELL CHECKS AND ADJUSTMENTS, 4.4-1

Purpose, 4.4-1
Preparation, 4.4-1
Flow Cell Checks, 4.4-1
DIFF Lamp Voltage Adjustment, 4.4-3
Preparation, 4.4-3
Adjustment, 4.4-3
Interim Verification, 4.4-4
Resistive Channel Adjustment, 4.4-5
Preparation, 4.4-5
Adjustment, 4.4-6
Absorbance Channel Adjustment, 4.4-7
Preparation, 4.4-7
Adjustment, 4.4-7
Final Verification, 4.4-9
4.5 BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT, 4.5-1

Purpose, 4.5-1
Preparation, 4.5-1
Alignment Check, 4.5-1
Alignment Adjustment, 4.5-3
Verification, 4.5-4
4.6 SAMPLE PROBE CHECKS AND ADJUSTMENTS, 4.6-1

Purpose, 4.6-1
Sample Probe Checks, 4.6-2
Home Position Check, 4.6-2
Inside Bath Position Check, 4.6-3
Sample Probe Adjustments, 4.6-4
Home Position Adjustment, 4.6-4
Inside Bath Position Adjustment, 4.6-5
4.7 HGB BLANK ADJUSTMENT, 4.7-1

Purpose, 4.7-1
Preparation, 4.7-1
Adjustment, 4.7-2
Verification, 4.7-2
4.8 APERTURE CURRENT CHECK, 4.8-1

Purpose, 4.8-1
x PN 4237616B
CONTENTS
Procedure, 4.8-1
4.9 RBC/PLT GAIN ADJUSTMENT, 4.9-1

Purpose, 4.9-1
Procedure, 4.9-1
Preparation, 4.9-1
Adjustments, 4.9-2
4.10 WBC/BASO GAIN ADJUSTMENT, 4.10-1

Purpose, 4.10-1
Procedure, 4.10-1
Preparation, 4.10-1
Adjustment, 4.10-2
4.11 DRAIN SENSOR ADJUSTMENT, 4.11-1

Purpose, 4.11-1
Preparation, 4.11-1
Adjustment, 4.11-2
Wrap Up, 4.11-2
4.12 TRANSFER SENSOR ADJUSTMENT, 4.12-1

Purpose, 4.12-1
Preparation, 4.12-1
Adjustment, 4.12-2
Wrap Up, 4.12-2
4.13 MOTOR CURRENT ADJUSTMENT, 4.13-1

Purpose, 4.13-1
Procedure, 4.13-1
4.14 THRESHOLD ADJUSTMENTS, 4.14-1

Purpose, 4.14-1
Procedure, 4.14-1
4.15 REAGENT TEMPERATURE CHECK AND ADJUSTMENT, 4.15-1

Purpose, 4.15-1
Reagent Temperature Check, 4.15-1
Reagent Temperature Adjustment, 4.15-3
Preparation, 4.15-3
Adjustment, 4.15-4
4.16 BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT, 4.16-1

Purpose, 4.16-1
PN 4237616B xi
CONTENTS
Bath Enclosure Temperature Check, 4.16-1

Bath Enclosure Temperature Adjustment, 4.16-2
Preparation, 4.16-2
Adjustment, 4.16-3
4.17 VACUUM CHECKS AND ADJUSTMENTS, 4.17-1

Purpose, 4.17-1
Waste Syringe Vacuum Check, 4.17-1
Count Syringe Vacuum Check and Adjustment, 4.17-2
Count Syringe Vacuum Check, 4.17-2
Count Syringe Vacuum Adjustment, 4.17-3
4.18 MIX BUBBLE ADJUSTMENT, 4.18-1

Purpose, 4.18-1
Tools/Supplies Required, 4.18-1
Procedure, 4.18-1
4.19 HEATER ASSEMBLY REPLACEMENT, 4.19-1

Purpose, 4.19-1
Removal, 4.19-1
Installation, 4.19-5
4.20 POWER SUPPLY REPLACEMENT, 4.20-1

Purpose, 4.20-1
Removal, 4.20-1
4.21 START SWITCH REPLACEMENT, 4.21-1

Purpose, 4.21-1
Removal, 4.21-1
4.22 OPTICAL BENCH DISASSEMBLY AND REPLACEMENT, 4.22-1

Purpose, 4.22-1
Removal, 4.22-1
4.23 BAR-CODE READER TESTING AND CONFIGURATION, 4.23-1

Purpose, 4.23-1
Read Test, 4.23-1
Default Settings, 4.23-2
Codabar - Start/Stop Equality Check/Output, 4.23-4
Interleaved 2-of-5 Options, 4.23-5
4.24 REAGENT SYRINGES ASSEMBLY REPLACEMENTS, 4.24-1
xii PN 4237616B
CONTENTS
Purpose, 4.24-1
Preparation, 4.24-1
Removal, 4.24-2
O-Ring, Washer, and Piston Replacement, 4.24-2
4.25 COUNT SYRINGE COMPONENT REPLACEMENTS, 4.25-1

Purpose, 4.25-1
Preparation, 4.25-1
Removal, 4.25-2
O-ring, Washer, and Piston Replacement, 4.25-5
Piston Replacement, 4.25-5
O-ring and Washer Replacement Only, 4.25-6
4.26 SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT

REPLACEMENTS, 4.26-1
Purpose, 4.26-1
Preparation, 4.26-1
Removal, 4.26-1
Sample Probe Replacement, 4.26-2
Rinse Block Assembly Component Replacement, 4.26-2
4.27 WASTE SYRINGE COMPONENT REPLACEMENTS, 4.27-1

Purpose, 4.27-1
Preparation, 4.27-1
Removal, 4.27-2
4.28 CLEANING THE BATH ENCLOSURE, 4.28-1

Purpose, 4.28-1
Procedure, 4.28-1
4.29 5diff SYRINGE ASSEMBLY REPLACEMENTS, 4.29-1

Purpose, 4.29-1
Preparation, 4.29-1
Removal, 4.29-1
O-ring Replacements, 4.29-3
PN 4237616B xiii
CONTENTS
4.30 FLOW CELL COAXIAL CABLE REPLACEMENT, 4.30-1

Purpose, 4.30-1
Preparation, 4.30-1
Removal, 4.30-1
Replacement, 4.30-4
4.31 OPTICAL BENCH LAMP REPLACEMENT, 4.31-1

Purpose, 4.31-1
Preparation, 4.31-1
Removal, 4.31-3
Lamp Replacement, 4.31-4
4.32 DILUENT RESERVOIR REPLACEMENTS, 4.32-1

Purpose, 4.32-1
Preparation, 4.32-1
Removal, 4.32-1
O-ring and Washer Replacement, 4.32-2
4.33 SAMPLE SYRINGE ASSEMBLY REPLACEMENTS, 4.33-1

Purpose, 4.33-1
Preparation, 4.33-1
Removal, 4.33-1
4.34 DRAINING BATH REPLACEMENTS, 4.34-1

Purpose, 4.34-1
Preparation, 4.34-1
4.35 O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and WBC/BASO

Baths), 4.35-1
Purpose, 4.35-1
Preparation, 4.35-1
Removal, 4.35-2
xiv PN 4237616B
CONTENTS
Replacing the Coaxial Cable O-ring on the Bath Electrode, 4.35-3

Replacing the Aperture O-rings in the Counting Head, 4.35-5
Align the Bath Assembly, 4.35-8
4.36 OPTICAL BENCH PRELIMINARY ADJUSTMENTS, 4.36-1

Purpose, 4.36-1
Preparation, 4.36-1
Verify the Flow Cell is Free of Bubbles, 4.36-2
Course Adjustments, 4.36-2
Y-Axis Adjustment, 4.36-2
X-Axis Adjustment, 4.36-3
Lamp Alignment, 4.36-5
4.37 FLOW CELL WBC BALANCE, 4.37-1

Purpose, 4.37-1
Procedure, 4.37-1
4.38 SETTING diff+/diff- THRESHOLDS, 4.38-1

Purpose, 4.38-1
Procedure, 4.38-1
4.39 MAIN CARD REPLACEMENT AND SOFTWARE TRANSFER, 4.39-1

Purpose, 4.39-1
Preliminary Setup, 4.39-1
Removing Main Card, 4.39-1
Transferring EPROMs to Replacement Card, 4.39-3
Installing Main Card, 4.39-4
Verifying System Configuration, 4.39-5
Patient Ranges, Action Ranges and Thresholds, 4.39-6
Verifying Main Card Settings, 4.39-7
5 MAINTENANCE PROCEDURES, 5.1-1
5.1 RECOMMENDED MAINTENANCE SCHEDULE, 5.1-1
5.2 MAINTENANCE WORKLIST, 5.2-1

Purpose, 5.2-1
Additional Tools or Supplies Needed for 1-Year Maintenance, 5.2-1
Additional Tools or Supplies Needed for the Every 2-Years Maintenance, 5.2-1
User Mode, 5.2-1
Worklist Instructions, 5.2-2
Overview, 5.2-2
Maintenance Category Identifier, 5.2-2
Basic Instructions, 5.2-2
PN 4237616B xv
CONTENTS
Preparation, 5.2-3
Reagent Syringes Assembly, 5.2-4
Replacement Parts, 5.2-4
Procedure, 5.2-4
5diff Syringe Assembly, 5.2-5
Procedure, 5.2-5
Count Syringe Assembly, 5.2-5
Procedure, 5.2-5
Interim Verification Check, 5.2-6
Replace the Optical Bench Lamp, 5.2-6
Replacement Part, 5.2-6
Procedure, 5.2-6
Replace the Flow Cell Coaxial Cable, 5.2-7
Procedure, 5.2-7
Preparation, 5.2-7
Sample Probe and Rinse Block Assembly, 5.2-8
Procedure, 5.2-8
Sample Syringe Assembly, 5.2-8
Procedure, 5.2-8
Waste Syringe Assembly, 5.2-9
Procedure, 5.2-9
Diluent Reservoir, 5.2-9
Procedure, 5.2-9
Preparation, 5.2-10
Draining Baths, 5.2-11
Procedure, 5.2-11
Counting Heads, 5.2-11
Procedure, 5.2-11
Clean the Bath Enclosure, 5.2-11
Wrap Up, 5.2-12
5.3 SYSTEM VERIFICATION PROCEDURES, 5.3-1

Purpose, 5.3-1
Preparation, 5.3-1
Startup, 5.3-1
Reproducibility, 5.3-1
Calibration, 5.3-2
Autocalibration, 5.3-2
Run Calibration, 5.3-2
xvi PN 4237616B
CONTENTS
Calibration Passed, 5.3-2

Step-By-Step Cycle Check, 5.3-3
Preparation, 5.3-3
Instrument At Rest, 5.3-3
Sample Preparation (Making the Dilutions), 5.3-3
Count and Measurement of the WBC Group, 5.3-5
RBC/PLT Group Count, 5.3-6
Permanent Rinse Flow (PRF), 5.3-6
Filling of Diluent Reservoir, 5.3-6
Completing the Cycle, 5.3-6
Wrap Up, 5.3-6
6 SCHEMATICS, 6.1-1
6.1 ENGINEERING SCHEMATIC DRAWINGS, 6.1-1

Engineering Schematics, 6.1-1
Additional Pneumatic/Hydraulic Information, 6.1-1
Tubings and Connectors, 6.1-1
Pneumatic/Hydraulic Circuit Connections, 6.1-1
Additional Interconnect Information, 6.1-1
6.2 PNEUMATIC / HYDRAULIC SCHEMATIC, 6.2-1

Layout, 6.2-1
Color Coding, 6.2-1
Tubing Designations, 6.2-1
Solenoid Valves, 6.2-1
6.3 PNEUMATIC/HYDRAULIC TUBINGS AND CONNECTIONS, 6.3-1

Tubings and Connectors List, 6.3-1
6.4 INTERCONNECT DIAGRAM, 6.4-1
6.5 MOTORS AND CABLES, 6.5-1
7 TROUBLESHOOTING, 7.1-1
7.1 ERROR MESSAGES, 7.1-1
7.2 CHECKING THE MOTORS, 7.2-1

Purpose, 7.2-1
Preparation, 7.2-2
To Check All Motors, 7.2-2
To Only Check Motors in the Right Side Compartment, 7.2-2
To Only Check Motors in the Left Side Compartment, 7.2-2
Motors Check, 7.2-3
Wrap Up, 7.2-3
7.3 CHECKING THE VALVES, 7.3-1

Purpose, 7.3-1
PN 4237616B xvii
CONTENTS
Preparation, 7.3-1
To Only Check Valves in the Left Side Compartment, 7.3-1
To Only Check Valves in the Right Side Compartment, 7.3-1
Valves Check, 7.3-2
Wrap Up, 7.3-3
8 PARTS LISTS, 8.1-1
8.1 MASTER PARTS LISTS, 8.1-1
8.2 ILLUSTRATED PARTS, 8.2-1
A QUICK REFERENCE INFORMATION, A.1-1
A.1 TOLERANCE AND LIMITS, A.1-1
A.2 CIRCUIT CARD LAYOUTS WITH KEY COMPONENT DESCRIPTIONS, A.2-1

Main Card, A.2-1
Component Locations, A.2-1
Test Points, A.2-2
Potentiometers, A.2-4
Jumper Settings, A.2-5
Optical Preamplifier Card, A.2-6
Connectors, A.2-6
LCD and Keypad Card, A.2-7
LED Card, A.2-8
Connectors, A.2-8
Motor Interconnect Card, A.2-9
Connectors, A.2-9
Traverse Card, A.2-10
Connectors, A.2-10
A.3 AC•T 5diff MODULE LOCATIONS AND FUNCTIONS, A.3-1

Overview, A.3-1
Analyzer Modules, A.3-1
Mechanical and Hydraulic Modules Locations, A.3-2
Rear Panel, A.3-4
A.4 SOFTWARE MENU TREE, A.4-1
B SOFTWARE INTERFACE, B.1-1
B.1 FORMAT, B.1-1
B.2 PACKET TYPE PIN ASSIGNMENTS, B.2-1
C FLAG SENSITIVITY AND THRESHOLDS, C.1-1
C.1 OVERVIEW, C.1-1

Flag Sensitivity, C.1-1
xviii PN 4237616B
CONTENTS
Thresholds, C.1-2
Plt Threshold, C.1-2
WBC and BASO Thresholds, C.1-2
DiffPlot Thresholds, C.1-3
DiffPlot - Volume Thresholds, C.1-4
DiffPlot - Absorbance Thresholds, C.1-6
NL, NE, and MN Alarms, C.1-7
RBC Histogram, C.1-7
C.2 SETTING FLAG SENSITIVITY, C.2-1

Purpose, C.2-1
Tools/Supplies Needed, C.2-1
Procedure, C.2-1
Verification, C.2-1
C.3 SETTING THRESHOLDS, C.3-1

Purpose, C.3-1
Procedure, C.3-1
Verification, C.3-2
ABBREVIATIONS, ABBREVIATIONS-1
INDEX, 1
TRADEMARKS, 1
PN 4237616B xix
CONTENTS
ILLUSTRATIONS
1.2-1 Warning and Information Label, 1.2-2
2.1-1 User Interfaces on the AC•T 5diff Hematology Analyzer, 2.1-2
2.2-1 Coulter Principle, 2.2-2
2.2-2 Dual Focused Flow Process, 2.2-3
2.2-3 Signal Processing, 2.2-4
2.2-4 Basophil Thresholds, 2.2-5
2.2-5 Bath Assembly, 2.2-6
2.2-6 Sample Delivery Using Tangential Flow, 2.2-6
2.2-7 CBC/DIFF Mode -
Sample Partitions inside the Probe, 2.2-7
2.2-8 CBC Mode -
2.3-1 Sample Probe and LED at Start of a Cycle, 2.3-1
2.3-2 Baths Assembly at Start of a Cycle, 2.3-1
2.3-3 Rinsing Probe Exterior After Aspiration, 2.3-2
2.3-4 Making the RBC/PLT First Dilution, 2.3-2
2.3-5 Making the WBC/BASO Dilution, 2.3-3
2.3-6 Making the DIFF Bath Dilution, 2.3-3
2.3-7 Double Rinse of the Sample Probe, 2.3-4
2.3-8 Aspirating from the First Dilution, 2.3-4
2.3-9 Rinsing the Outside of the Probe, 2.3-5
2.3-10 Making the RBC/Plt Dilution, 2.3-5
2.4-3 Flow Cell Operation, 2.4-5
2.4-4 DiffPlot Regions, 2.4-6
2.5-1 Typical RBC Histogram, 2.5-1
2.5-2 RBC1 and RBC2 Positions - RBC Histogram, 2.5-2
2.6-1 Typical Plt Histogram, 2.6-1
2.6-2 Area of the Plt Histogram Used to Determine the PDW Parameter Result, 2.6-2
2.6-3 Typical Platelet Distribution Curve, 2.6-3
2.6-4 Microcytic Interference with a Valley between 18 fL and 25 fL, 2.6-4
2.6-5 Microcytic Interference with a Valley below 18 fL, 2.6-4
2.6-6 Interference with no Distinct Valley, 2.6-5
2.7-1 Areas Used to Determine WBC and BASO Parameter Results, 2.7-1
2.7-3 Volume Thresholds, 2.7-5
2.7-4 Absorbance Thresholds / NL, NE and MN Alarms, 2.7-6
2.8-1 Valve 1 through Valve 16 Locations, 2.8-1
2.8-2 Valve 17 and 18 Location, 2.8-1
2.8-3 Valve 20 to Valve 31 Locations, 2.8-2
2.8-4 Hgb Lyse Reagent Circuit, 2.8-5
2.8-5 Fix Reagent Circuit, 2.8-6
2.8-6 WBC Lyse Reagent Circuit, 2.8-7
2.8-7 Rinse Reagent Supply Circuit, 2.8-8
2.8-8 Probe Rinse Reagent Circuit, 2.8-9
2.8-9 WBC/BASO Rinse Reagent Circuit, 2.8-10
2.8-10 Diluent Reagent Circuit, 2.8-11
xx PN 4237616B
CONTENTS
2.8-11 Probe Diluent Reagent Circuit, 2.8-13

2.8-12 Bath Diluent Reagent Circuit, 2.8-14
2.8-13 Waste Circuit, 2.8-15
2.10-1 User Menu Tree, 2.10-2
2.10-2 Service Menu Tree, 2.10-3
3.2-1 Warning and Caution Label Locations on the Instrument, 3.2-2
3.2-2 Plastic Blocker Locations, View with Right Door Open, 3.2-2
3.2-3 Rear Panel Connections, 3.2-3
3.2-4 Loop-Side Velcro Strip Attachment, 3.2-4
3.2-5 Waste Alarm and Float Sensor Setup, 3.2-4
3.2-6 Position the Waste Alarm on the Rear Access Panel, 3.2-5
3.2-7 Reagent Bottle Locations, 3.2-6
3.2-8 Printer Configuration Menu, 3.2-9
3.3-1 Carefully Remove All Packing Materials, 3.3-1
3.3-2 Paper-Feed Knob Installation, 3.3-1
3.3-3 Insert the Ribbon Cartridge, 3.3-2
3.3-4 Threading the Ribbon, 3.3-2
3.3-5 Proper Ribbon Placement, 3.3-3
3.3-6 Cable Connection at the Instrument, 3.3-3
3.3-7 Cable Connections, 3.3-4
3.3-8 Paper Support for Printing Single Sheets of Paper, 3.3-5
3.3-9 Printer Sprockets, 3.3-5
3.3-10 Replace Paper Guide, 3.3-6
3.3-11 Printer Ready for Continuous Feed Printing, 3.3-6
3.3-12 LX300 Printer Control Panel, 3.3-7
3.3-13 LX300+ Printer Control Panel, 3.3-7
4.1-1 Location of the Power On/Off Rocker Switch, 4.1-3
4.2-1 Opening the Right Side Door, 4.2-1
4.2-2 Removing the Left Side Panel, 4.2-3
4.2-3 Rear Access Panel Screw Locations, 4.2-4
4.2-4 Top Cover - Side Screw Locations, 4.2-5
4.2-5 Reagent Compartment Screw Locations, 4.2-6
4.2-6 Torx Screw Location Inside Front Panel, 4.2-6
4.2-7 Torx Screw Location on Right Frame, 4.2-6
4.2-8 Front Panel Screw Locations, 4.2-7
4.2-9 Connector for the Keypad and LCD Card, 4.2-7
4.4-1 Diff Adjustment Screen, 4.4-2
4.4-2 Potentiometer R11 - Location on the Optical Bench Assembly, 4.4-3
4.4-3 Potentiometer R11 Location - Top View from the Front of the Instrument, 4.4-3
4.4-4 Main Card Flow Cell Adjustments, 4.4-5
4.4-5 Front Adjustment Knob - Optical Bench, 4.4-7
4.4-6 Side Adjustment Screw - Optical Bench, 4.4-7
4.5-1 Sample Probe Position at the Rinse Bath, 4.5-2
4.5-2 Close-up of Probe Position, 4.5-2
4.5-3 Sample Probe at the WBC/BASO Bath, 4.5-2
4.5-4 Close-up of Probe at the WBC/BASO Bath, 4.5-2
4.5-5 Location of Screws Securing the Baths Support Panel, 4.5-3
PN 4237616B xxi
CONTENTS
4.5-7 Close-up of Acceptable Probe Position, 4.5-3

4.5-8 Sample Probe Position, Right Side, 4.5-4
4.6-1 Dilution Screen, 4.6-1
4.6-2 Acceptable Distance between the Sample Probe Tip and the Traverse, 4.6-2
4.6-3 Measuring the Distance between the Sample Probe Tip and the Traverse, 4.6-2
4.6-4 Correct View through the Port, 4.6-3
4.6-5 Incorrect View through the Port, 4.6-3
4.6-6 Properly Adjusted Sample Probe Tip, 4.6-4
4.6-7 Ideal Probe Position, 4.6-5
4.6-8 Improper Probe Tip Position - Too Forward, 4.6-5
4.6-9 Improper Probe Tip Position - Too Backward, 4.6-5
4.6-10 Improper Probe Tip Position - Too High, 4.6-6
4.6-11 Improper Probe Tip Position - Too Low, 4.6-6
4.7-1 Main Card Hgb Blank Adjustment, 4.7-1
4.8-1 LMNE CIS, GR (RBC), and GB (WBC) Coax Locations, 4.8-1
4.9-1 Main Card RBC/PLT Gain Adjustments, 4.9-1
4.10-1 Main Card WBC/BASO Gain Adjustment, 4.10-1
4.11-1 Main Card Drain Sensor Adjustment, 4.11-1
4.12-1 Main Card Transfer Sensor Adjustment, 4.12-1
4.13-1 Main Card Motor Current Adjustments, 4.13-2
4.14-1 Main Card Threshold Adjustments, 4.14-2
4.15-1 Thermometer Probe inside the DIFF Bath, 4.15-1
4.15-2 Main Card Heating Status LED Location, 4.15-2
4.15-3 Location of the Label Containing the Temperature Value for the Heater
Assembly, 4.15-3
4.16-1 Temperature Sensor Location - View with the Right Side Door Open, 4.16-1
4.16-2 Location of the Label Containing the Temperature Value for the Temperature
Sensor, 4.16-2
4.17-1 Attach the Vacuum Meter to the Waste Syringe, 4.17-1
4.17-2 Attach the Vacuum Meter to the Count Syringe, 4.17-2
4.19-1 Captive Hex Screw Locations - Reagent Syringes and 5diff Syringe, 4.19-1
4.19-2 CHC M3 x 6 Screw Locations, 4.19-2
4.19-3 Heater Assembly, 4.19-3
4.19-4 Heater Assembly - Tubing Port Locations, 4.19-3
4.19-5 Main Card Heater Assembly Replacement, 4.19-4
4.19-6 Heater Assembly After Removal, 4.19-5
4.19-8 Heater Assembly - Orientation Inside the Instrument, 4.19-6
4.19-9 Heater Assembly - Port Locations, 4.19-6
4.19-10Baths Assembly Support Panel Nut Locations, 4.19-7
4.19-11Location of Openings in the Baths Assembly Support Panel, 4.19-7
4.19-12Heater Assembly Screw Locations, 4.19-8
4.20-1 Main Card - J2 Location, 4.20-1
4.20-3 Optical Module - Lamp Supply Cable Location, 4.20-2
4.20-4 Location of Screws Securing the Power Supply to the Rear Panel, 4.20-3
4.20-5 Screws Securing the Power Supply to the Instrument Frame, 4.20-3
4.20-6 Main Card Heater Assembly Replacement Adjustment, 4.20-4
xxii PN 4237616B
CONTENTS
4.21-1 Fan Removal - Right Side Compartment, 4.21-1

4.21-2 Start Switch Screw Locations - With Fan Removed, 4.21-2
4.21-3 Disconnected Start Switch - Front View with Front Panel Removed, 4.21-2
4.21-4 Start Switch Orientation, 4.21-3
4.22-1 5diff Syringe Port Locations, 4.22-1
4.22-2 Optical Bench - Ground Fitting Location, 4.22-2
4.22-3 Disconnection Sites for Named Components, 4.22-2
4.22-4 Captive Screw Locations - Optical Bench Assembly, 4.22-3
4.22-5 Connection Sites for Named Components, 4.22-4
4.24-1 Valve and Screw Locations - Left Side View, 4.24-2
4.24-2 O-rings and Washers - Reagent Syringes Assembly, 4.24-3
4.24-3 Bottom Plate Screw Locations and Tightening Patterns, 4.24-4
4.25-2 Motor Interconnect Card - Count Syringe Connector Locations, 4.25-2
4.25-3 Count Syringe - Ground Wire Location, 4.25-3
4.25-4 Count Syringe Housing - Tubing Locations, 4.25-3
4.25-5 Count Syringe - Captive Screw Locations, 4.25-4
4.25-6 Count Syringe - Piston, O-ring, and Washer Replacement, 4.25-5
4.25-7 Count Syringe - O-ring and Washer Replacement, 4.25-6
4.26-1 Probe Rinse Block Screw Locations, 4.26-1
4.26-2 Lift the Sample Probe Lock-Lever, 4.26-2
4.26-3 Remove Probe and Rinse Block Assembly, 4.26-2
4.26-4 Reassemble the Rinse Block Assembly, 4.26-3
4.27-2 Motor Interconnect Card - Waste Syringe Connector Locations, 4.27-2
4.27-3 Waste Syringe - Tie Wrap and Ground Wire Location, 4.27-3
4.27-4 Waste Syringe - Captive Screw Locations, 4.27-4
4.27-5 Waste Syringe - Piston, O-ring, and Washer Replacement, 4.27-5
4.27-6 Waste Syringe - O-ring and Washer Replacement, 4.27-6
4.29-2 Valve and Tubing Locations - Left Side View, 4.29-2
4.29-3 Screw Locations - 5diff Syringe, 4.29-3
4.29-4 Bottom Plate - 5diff Syringe, 4.29-3
4.29-5 Bottom Plate and Attachments - Housing Removed, 4.29-4
4.29-6 Illustrated Parts - 5diff Syringe, 4.29-5
4.30-1 Optical Bench Cover Screw Locations, 4.30-1
4.30-2 T-Connector Location, 4.30-2
4.30-3 Disconnecting the Isolator Chamber from the T-connector, 4.30-2
4.30-4 Coaxial Cable Connector - Top View, 4.30-3
4.30-5 Flow Cell Screw Locations - Top View, 4.30-3
4.30-6 Ground Screw Removal, 4.30-4
4.30-7 Connector Location - Top View, 4.30-5
4.31-1 Optical Bench Lamp Assembly, 4.31-2
4.31-2 Power Connector Location - Top View, 4.31-3
4.31-3 Screw Locations - Lamp Housing, 4.31-3
4.31-4 Winged Metal Bracket - Top View, 4.31-4
PN 4237616B xxiii
CONTENTS
4.32-1 Diluent Reservoir Screw Locations, 4.32-1

4.32-2 Diluent Reservoir O-ring and Washer Positioning, 4.32-2
4.33-1 Disconnect Tubing at Valve 18, Port 2, 4.33-1
4.33-2 Screw Locations - Sample Syringe Housing, 4.33-2
4.33-3 Sample Syringe Output Port, 4.33-2
4.33-4 Screw Locations on the Bottom of the Sample Syringe Housing, 4.33-3
4.33-5 Illustrated Parts - Sample Syringe, 4.33-4
4.34-1 Removing a Draining Bath, 4.34-1
4.34-2 Draining Bath O-ring Placement, 4.34-2
4.34-3 Draining Bath O-Ring Locations, 4.34-2
4.35-2 Close-up of Probe Positioning, 4.35-2
4.35-3 Baths Assembly Screw Locations, 4.35-3
4.35-4 Electrode Screw Locations, 4.35-3
4.35-5 Removing the Coaxial Cable O-ring from the Bath Electrode, 4.35-4
4.35-6 Making a Protective Cover from a Micropipette Tip, 4.35-4
4.35-7 Bath Electrode Locations, 4.35-5
4.35-8 Location of the Aperture and Its Two O-Rings, 4.35-6
4.35-10Close-up of Acceptable Probe Position, 4.35-8
4.35-11Sample Probe Position, Right Side, 4.35-9
4.36-2 Checking the Lens to Flow Cell Gap, 4.36-2
4.36-3 Front Knob for Y-Axis Adjustment, 4.36-3
4.36-4 Side Screw - X-Axis Adjustment, 4.36-3
4.36-5 How to Position the White Paper, 4.36-4
4.36-6 Ideal Lamp Filament Projection Image, 4.36-4
4.36-8 Lamp Adjustment Screw, 4.36-5
4.36-10Side Screw - X-Axis Adjustment, 4.36-6
4.37-1 WBC/Flow Cell Balance Screen, 4.37-1
4.38-1 diff Flag Sensitivity Screen, 4.38-1
4.39-1 Main Card - Connections and EPROMs, 4.39-2
4.39-2 Main Card - Potentiometers /Test Points, 4.39-9
6.5-1 Horizontal Traverse Motor (PN - XBA391A), 6.5-1
6.5-2 Upper Fan (PN - XBA393A), 6.5-2
6.5-3 Horizontal Traverse Sensor (IR Sensor, PN - XBA394AS), 6.5-3
6.5-4 Bath Drain and DIFF Transfer Sensor (IR Sensor, PN - XBA395AS), 6.5-4
6.5-5 Vertical Traverse Sensor (IR Sensor, PN - XBA396AS), 6.5-5
6.5-6 IR Sensor (PN - XBA397AS), 6.5-6
6.5-7 RBC/WBC Coaxial Cable (PN - XBA398A), 6.5-7
6.5-8 DIFF Flow Cell Coaxial Cable (PN - XBA399AS), 6.5-8
6.5-9 Bar-Code Reader Cable (PN - XBA402AS), 6.5-9
6.5-10 Diluent Level Sensor (PN - XDA605AS), 6.5-10
7.3-1 Valve 1 through 16 Locations, 7.3-2
7.3-2 Valves 17 and 18 Location, 7.3-2
7.3-3 Valves 20 through 31 Locations, 7.3-2
xxiv PN 4237616B
CONTENTS
8.2-1 11-Valves Assembly (See Table 8.2-1), 8.2-1

8.2-6 Right Side Compartment, Lower Rear Area (See Table 8.2-6), 8.2-6
8.2-7 Diluent Reservoir Assembly (See Table 8.2-7), 8.2-7
8.2-8 Count Syringe and Motor Assembly (See Table 8.2-8), 8.2-8
8.2-9 Count Syringe Assembly (See Table 8.2-9), 8.2-9
8.2-10 Count Syringe Motor Assembly (See Table 8.2-10), 8.2-10
8.2-11 Count Syringe Piston Assembly (See Table 8.2-11), 8.2-11
8.2-12 Reagent Syringes and Motor Assembly (See Table 8.2-12), 8.2-12
8.2-13 Reagent Syringes Assembly (See Table 8.2-13), 8.2-13
8.2-14 5diff Syringe and Motor Assembly (See Table 8.2-14), 8.2-14
8.2-15 5diff Syringe Assembly (See Table 8.2-15), 8.2-15
8.2-16 Waste Syringe and Motor Assembly (See Table 8.2-16), 8.2-16
8.2-17 Waste Syringe Assembly (See Table 8.2-17), 8.2-17
8.2-18 Syringe Motor (See Table 8.2-18), 8.2-18
8.2-19 Sample Motor (See Table 8.2-19), 8.2-19
8.2-20 Sample Syringe and Motor Assembly (See Table 8.2-20), 8.2-20
8.2-21 Sample Assembly Syringe (See Table 8.2-21), 8.2-21
8.2-22 Sample Syringe Motor Assembly (See Table 8.2-22), 8.2-22
8.2-23 Syringe Motor Housing Assembly (See Table 8.2-23), 8.2-23
8.2-24 Syringe Motor Guide Block Assembly (See Table 8.2-24), 8.2-24
8.2-25 Sample Probe Retainer and Guide Assembly (See Table 8.2-25), 8.2-25
8.2-26 Sample Probe Retainer (See Table 8.2-26), 8.2-26
8.2-27 Rinse Block Assembly (See Table 8.2-27), 8.2-27
8.2-28 Sample Probe (See Table 8.2-28), 8.2-28
8.2-29 Traverse Vertical Movement Components - Belt Retainer (See
Table 8.2-29), 8.2-29
8.2-30 Vertical Traverse Vertical Movement Components - Belt (See
Table 8.2-30), 8.2-30
8.2-31 Traverse Vertical Movement Components - Motor and Pulley (See
Table 8.2-31), 8.2-31
8.2-32 Traverse Vertical Movement Components - Motor (See Table 8.2-32), 8.2-32
8.2-33 Traverse Vertical Movement Components - Home Sensor (See
Table 8.2-33), 8.2-33
8.2-34 Traverse Horizontal Movement Components - Motor (See Table 8.2-34), 8.2-34
8.2-35 Traverse Horizontal Movement Components - Belt (See Table 8.2-35)t, 8.2-35
8.2-36 Traverse Horizontal Movement Components - Free Wheel and Home Sensor
(See Table 8.2-36), 8.2-36
8.2-37 Optical Bench Assembly (See Table 8.2-37), 8.2-37
8.2-38 Optical Bench Lamp (See Table 8.2-38), 8.2-38
8.2-39 DIFF Flow Cell Assembly (See Table 8.2-39), 8.2-39
8.2-40 Optics Preamplifier (See Table 8.2-40), 8.2-40
8.2-41 LED Card (See Table 8.2-41), 8.2-41
8.2-42 Bath Enclosure Compartment (See Table 8.2-42), 8.2-42
8.2-43 Bath Enclosure Fan Assembly (See Table 8.2-43), 8.2-43
8.2-44 Bath Enclosure Door Interlock (See Table 8.2-44), 8.2-44
8.2-45 Reagent Heating Coil Assembly (See Table 8.2-45), 8.2-45
PN 4237616B xxv
CONTENTS
8.2-46 Baths Assembly (See Table 8.2-46), 8.2-46

8.2-47 Hgb Photometer Assembly (See Table 8.2-47), 8.2-47
8.2-48 WBC/BASO Bath Assembly (See Table 8.2-48), 8.2-48
8.2-49 Rear Frame Assembly (See Table 8.2-49), 8.2-49
A.2-1 Main Card Components, A.2-1
A.2-2 Main Card Jumper Settings, A.2-5
A.2-3 Optical Preamplifier Card Components, A.2-6
A.2-4 Keypad and LCD Card Components, A.2-7
A.2-5 LED Card Components, A.2-8
A.2-6 Motor Interconnect Card Components, A.2-9
A.2-7 Traverse Card Components, A.2-10
A.3-1 View of an AC•T 5diff Hematology Analyzer with the Right Side Door
Open, A.3-2
A.3-2 View of an AC•T 5diff Hematology Analyzer with the Left Side Panel
Removed, A.3-2
A.3-3 Rear Panel - AC•T 5diff Hematology Analyzer, A.3-4
A.4-1 Software Menu Tree , A.4-1
C.1-1 PLT Threshold, C.1-2
C.1-2 WBC and BASO Threshold, C.1-2
C.1-3 DiffPlot, C.1-3
C.1-4 DiffPlot - Volume Thresholds (Y-axis), C.1-4
C.1-5 DiffPlot - Absorbance Thresholds (X-Axis), C.1-6
C.2-1 Flags Sensitivity Screen, C.2-1
C.3-1 Thresholds Screen, C.3-1
xxvi PN 4237616B
CONTENTS
TABLES
2.1-1 AC•T 5diff Hematology Analyzer Reagent Consumption, Software Version
1.03, 2.1-4
2.2-1 C
A •T 5diff Analyzer Measurement Technologies, 2.2-1
2.4-1 Technical Characteristics for Obtaining RBC and Platelet Counts, 2.4-2
2.4-2 Technical Characteristics for the Measurement of the Hemoglobin, 2.4-3
2.4-3 Characteristics Required to Obtain WBC and BASO Results, 2.4-4
2.4-4 Technical Characteristics for Acquisition of the DiffPlot, 2.4-6
2.4-5 Summary of Dilutions, 2.4-7
2.8-1 Valves and their Functions, 2.8-3
3.1-1 Space Requirements, 3.1-1
3.2-1 Whole-Blood Reproducibility CV Limits for 20 Cycles, 3.2-10
3.2-2 Calibration Factors - Acceptable Range, 3.2-11
3.3-1 LX300+ Printer Controls and Indicators, 3.3-7
3.3-2 LX300+ Printer Default Settings, 3.3-8
4.13-1 Motor Voltage Limits, 4.13-1
4.14-1 Threshold Voltage Limits, 4.14-1
4.18-1 Mixing Bubble Limits, 4.18-1
4.20-1 Power Supply Voltages, 4.20-4
4.23-1 Test Labels With Check Digit (Checksum), 4.23-1
4.23-2 Test Labels Without Check Digit, 4.23-2
4.23-3 Bar-code Labels for Default Configuration, 4.23-3
4.39-1 Main Card - Plug/Jack Connections, 4.39-3
4.39-2 AC•T 5diff Menu Paths - System Settings, 4.39-5
4.39-3 AcT 5diff - Main Card Settings, 4.39-7
5.1-1 Maintenance Schedule, 5.1-1
6.3-1 Instrument Tubing and Connectors , 6.3-1
6.3-2 Circuit Connections , 6.3-3
7.1-1 Error Messages, 7.1-1
8.1-1 Part Categories, 8.1-1
8.1-2 Return Parts, 8.1-1
8.1-3 Nonreturn Parts, 8.1-2
8.1-4 Peripherals, Accessories and Consumables, 8.1-6
8.1-5 Tools, 8.1-7
8.1-6 Fitting Kit Parts PN - XEA311AS, 8.1-8
8.1-7 Screws Kit Parts PN - XEA293AS, 8.1-8
8.1-8 Installation Kit, PN - XEA484AS, 8.1-10
8.1-9 Waste Alarm Kit, PN - 6912680, 8.1-11
8.1-10 6 Month Maintenance Kit, PN - XEA485AS, 8.1-11
8.1-11 1 Year Maintenance Kit, PN - XEA486AS, 8.1-11
8.1-12 Every 2 Years Maintenance Kit, PN - XEA581AS, 8.1-11
8.1-13 100 mN-m Torque Driver Kit, - PN 6915456, 8.1-12
8.1-15 Assorted Tools Kit, - PN 6915458, 8.1-12
8.2-1 11-Valves Assembly (See Figure 8.2-1), 8.2-1
PN 4237616B xxvii
CONTENTS

8.2-6 Right Side Compartment, Lower Rear Area (See Figure 8.2-6), 8.2-6
8.2-7 Diluent Reservoir Assembly (See Figure 8.2-7), 8.2-7
8.2-8 Count Syringe and Motor Assembly (See Figure 8.2-8), 8.2-8
8.2-9 Count Syringe Assembly (See Figure 8.2-9), 8.2-9
8.2-10 Count Syringe Motor Assembly (See Figure 8.2-10), 8.2-10
8.2-11 Count Syringe Piston Assembly (See Figure 8.2-11), 8.2-11
8.2-12 Reagent Syringes and Motor Assembly (See Figure 8.2-12), 8.2-12
8.2-13 Reagent Syringes Assembly (See Figure 8.2-13), 8.2-13
8.2-14 5diff Syringe and Motor Assembly (See Figure 8.2-14), 8.2-14
8.2-15 5diff Syringe Assembly (See Figure 8.2-15), 8.2-15
8.2-16 Waste Syringe and Motor Assembly (See Figure 8.2-16), 8.2-16
8.2-17 Waste Syringe Assembly (See Figure 8.2-17), 8.2-17
8.2-18 Syringe Motor (See Figure 8.2-18), 8.2-18
8.2-19 Sample Motor (See Figure 8.2-19), 8.2-19
8.2-20 Sample Syringe and Motor Assembly (See Figure 8.2-20), 8.2-20
8.2-21 Sample Assembly Syringe (See Figure 8.2-21), 8.2-21
8.2-22 Sample Syringe Motor Assembly (See Figure 8.2-22), 8.2-22
8.2-23 Syringe Motor Housing Assembly (See Figure 8.2-23), 8.2-23
8.2-24 Syringe Motor Guide Block Assembly (See Figure 8.2-24), 8.2-24
8.2-25 Sample Probe Retainer and Guide Assembly (See Figure 8.2-25), 8.2-25
8.2-26 Sample Probe Retainer (See Figure 8.2-26), 8.2-26
8.2-27 Rinse Block Assembly (See Figure 8.2-27), 8.2-27
8.2-28 Sample Probe (See Figure 8.2-28), 8.2-28
Figure 8.2-29), 8.2-29
Figure 8.2-30), 8.2-30
Figure 8.2-31), 8.2-31
8.2-32 Traverse Vertical Movement Components - Motor (See Figure 8.2-32), 8.2-32
Figure 8.2-33), 8.2-33
8.2-34 Traverse Horizontal Movement Components - Motor (See Figure 8.2-34), 8.2-34
8.2-35 Traverse Horizontal Movement Components - Belt (See Figure 8.2-35), 8.2-35
(See Figure 8.2-36), 8.2-36
8.2-37 Optical Bench Assembly (See Figure 8.2-37), 8.2-37
8.2-38 Optical Bench Lamp (See Figure 8.2-38), 8.2-38
8.2-39 DIFF Flow Cell Assembly (See Figure 8.2-39), 8.2-39
8.2-40 Optics Preamplifier (See Figure 8.2-40), 8.2-40
8.2-41 LED Card (See Figure 8.2-41), 8.2-41
8.2-42 Bath Enclosure Compartment (See Figure 8.2-42), 8.2-42
8.2-43 Bath Enclosure Fan Assembly (See Figure 8.2-43), 8.2-43
8.2-44 Bath Enclosure Door Interlock (See Figure 8.2-44), 8.2-44
8.2-45 Reagent Heating Coil Assembly (See Figure 8.2-45), 8.2-45
8.2-46 Baths Assembly (See Figure 8.2-46), 8.2-46
8.2-47 Hgb Photometer Assembly (See Figure 8.2-47), 8.2-47
8.2-48 WBC/BASO Bath Assembly (See Figure 8.2-48), 8.2-48
xxviii PN 4237616B
CONTENTS
8.2-49 Rear Frame Assembly (Figure 8.2-49), 8.2-49

A.1-1 Flow Cell Adjustment Limits, A.1-1
A.1-2 Motor Voltage Limits, A.1-1
A.1-3 Thresholds Voltage Limits, A.1-1
A.1-4 Mixing Bubble Limits, A.1-1
A.1-5 Power Supply Voltages, A.1-2
A.1-6 Whole-Blood Reproducibility CV Limits for 20 Cycles, A.1-2
A.1-7 Calibration Factor Limits, A.1-2
A.2-1 Main Card Test Points , A.2-2
A.2-2 Main Card Potentiometers, A.2-4
A.2-3 Connectors on the Optical Preamplifier Card, A.2-6
A.2-4 Connectors on the Keypad and LCD Card, A.2-7
A.2-5 Connector on the LED Card, A.2-8
A.2-6 Connectors on the Motor Interconnect Card, A.2-9
A.2-7 Connectors on the Traverse Card, A.2-10
A.3-1 Mechanical and Hydraulic Modules, A.3-3
C.1-1 Flag Sensitivity Default Values, C.1-1
C.1-2 WBC/BASO Factory-Set Threshold Values, C.1-3
C.1-3 DiffPlot - Volume Thresholds (Y-Axis), C.1-5
C.1-5 DiffPlot - FNL, FNE, and FMN Thresholds, C.1-7
PN 4237616B xxix
CONTENTS
xxx PN 4237616B
CONTENTS
1
1 INTRODUCTION, 1.1-1
1.1 MANUAL DESCRIPTION, 1.1-1

Scope, 1.1-1
Notification of Updates, 1.1-1
Intended Audience, 1.1-1
Organization, 1.1-1
Numbering Format, 1.1-2
Special Headings, 1.1-3
WARNING, 1.1-3
CAUTION, 1.1-3
IMPORTANT, 1.1-3
ATTENTION, 1.1-3
Note, 1.1-3
Conventions, 1.1-3
Graphics, 1.1-4
1.2 SAFETY PRECAUTIONS, 1.2-1

Electronic, 1.2-1
Biological, 1.2-1
Troubleshooting, 1.2-1
ILLUSTRATIONS
1.2-1 Warning and Information Label, 1.2-2
PN 4237616B 1-i
CONTENTS
1-ii PN 4237616B
1INTRODUCTION 1
1.1 MANUAL DESCRIPTION
Scope
This manual provides the reference information and procedures needed for servicing and
maintaining the BECKMAN COULTER™ AC•T™ 5diff hematology analyzer (hereafter
referred to as the AC•T 5diff hematology analyzer or the instrument). It is available both
online and in hard copy. The online manual is released on the Service Resource Kit CD-ROM,
PN 6417471.
This manual is to be used in conjunction with the following customer documents and does
not contain information and procedures already covered in these documents:
Document Language Part Number

Operator’s Guide English 4237615
French 4237630
Italian 4237631
German 4237632
Spanish 4237633
Chinese 4237634
Host Transmission Specification English 4277065
Notification of Updates
Any service memo that affects the information in this manual will include either minor
revision change pages or a Notice of Information Update form for this manual. A Notice of
Information Update form will summarize the changes and will list the specific headings,
figures, and tables affected.
Intended Audience
To use this manual effectively, you need the following:
r An operator’s knowledge of the AC•T 5diff hematology analyzer
r A thorough understanding of -
t Basic electronic and pneumatic principles and devices
t Reagent systems
t Quality control
t Troubleshooting concepts
r The ability to -
t Use basic mechanical tools and understand related terminology
t Use a digital voltmeter (DVM)
t Read pneumatic/hydraulic schematics and understand related terminology
t Read electronic schematics and understand related terminology
Organization
The material in this manual is organized into eight chapters and two appendices. To make it
easier to access the information:
PN 4237616B 1.1-1
INTRODUCTION
MANUAL DESCRIPTION
r In the online manual, each page has a Contents button linked to a master table of
contents and an Index button linked to an alphabetic index.
r In the printed manual, there is a master table of contents at the beginning of the manual,
a chapter-specific table of contents at the beginning of each chapter, and an alphabetic
index at the end of the manual.
The chapters / appendices contain:
Chapter 1, INTRODUCTION - A brief description of this manual and essential safety

information.
Chapter 2, INSTRUMENT DESCRIPTION - An introduction to the AC•T5 diff hematology

analyzer and a description of how it functions.
Chapter 3, INSTALLATION PROCEDURES - Installation and verification procedures.
Chapter 4, SERVICE AND REPAIR PROCEDURES - The procedures for servicing/repairing

the AC•T 5diff hematology analyzer.
Chapter 5, MAINTENANCE PROCEDURES - The procedures for maintaining the

AC•T 5diff hematology analyzer.
Chapter 6, SCHEMATICS - The schematic diagrams and tubing lists.
Chapter 7, TROUBLESHOOTING - An error message table.
Chapter 8, PARTS LISTS - The master parts list followed by the illustrated parts list.
Appendix A, QUICK REFERENCE INFORMATION - Quick reference information:

tolerances and limits; connectors, test points and jumpers for the circuit cards; the software
menu trees; location diagrams and summarized functions for main analyzer components.
Appendix B, SOFTWARE INTERFACE - Tables of fatal and non-fatal error messages.
Appendix C, FLAG SENSITIVITY AND THRESHOLDS - An overview of the theory

including default values; also includes the setup procedures.
ABBREVIATIONS - A list of abbreviations, acronyms, and reference designators used in this

manual.
Numbering Format
Each chapter of this manual is further divided into topics that are numbered sequentially,
beginning at one. The numbering format for the topic heading, which is called the primary
heading, is chapter number, decimal point, topic number. For example, the primary heading
number for the third topic covered in Chapter 2 is 2.3.
The page, figure, and table numbers are tied directly to the primary heading number. For
example, Heading 2.3 begins on page 2.3-1, the first figure under Heading 2.3 is Figure 2.3-1
and the first table under Heading 2.3 is Table 2.3-1.
1.1-2 PN 4237616B
INTRODUCTION
MANUAL DESCRIPTION 1
Note: Primary headings always begin at the top of a right-hand page.
Special Headings
Throughout this manual, WARNING, CAUTION, IMPORTANT, ATTENTION, and Note
headings are used to indicate potentially hazardous situations and important or helpful
information.
WARNING
A WARNING indicates a situation or procedure that, if ignored, can cause serious personal
injury. The word WARNING is in bold-faced text in the printed manual and is red in the
online manual.
CAUTION
A CAUTION indicates a situation or procedure that, if ignored, can cause damage to the
instrument.The word CAUTION is in bold-faced text in the printed manual and is red in the
online manual.
IMPORTANT
An IMPORTANT indicates a situation or procedure that, if ignored, can result in erroneous
test results.The word IMPORTANT is in bold-faced text in the printed manual and is red in
the online manual.
ATTENTION
An ATTENTION contains information that is critical for the successful completion of a
procedure and/or operation of the instrument.The word ATTENTION is in bold-faced text in
the printed manual and is red in the online manual.
Note
A Note contains information that is important to remember or helpful in performing a
procedure.
Conventions
This manual uses the following conventions.
r Instrument or analyzer refers to the AC•T 5diff hematology analyzer.

r Main card refers to the motherboard in the instrument.
r Main Menu refers to the initial menu displayed on the instrument after Startup.
r Each menu option consists of an item number followed by bold, uppercase text. For
example, 3. REAGENTS is the third option on the Main Menu.
Note: Both the menu item number and text are displayed on the LCD screen. The item
number next to the menu item indicates the numeric pushbutton on the front of the
analyzer that can be pressed to select the menu option.
r Keys on the analyzer keypad are in bold, uppercase letters. For example, press ENTER
indicates the operator should press the ENTER pushbutton on the instrument keypad.
PN 4237616B 1.1-3
INTRODUCTION
MANUAL DESCRIPTION
r To select a menu item,

t Use the arrow keys to highlight the desired menu item then press the ENTER
pushbutton on the front of the analyzer to select the highlighted option.
or
t Simply press the numeric pushbutton (on the front of the analyzer) that correlates
with the desired option. This is the faster way to select a menu item.
For example, to select the 3. REAGENTS menu item from the Main Menu, you may:
t Use the down arrow to highlight the 3. REAGENTS option and then press the ENTER
pushbutton on the front of the analyzer.
or
t Press the pushbutton labeled 3 on the front of the analyzer.
r Select menu item tt sub-menu item indicates the software options you have to select, as
well as the order in which you should select them. For example, to prime the diluent
reagent:
From the Main Menu, select 3. REAGENTS tt 3. PRIME tt 1. DILUENT.
r Italics us used to indicate screen messages. For example:
The message CYCLE IN PROGRESS. PLEASE WAIT . . . appears on the screen.
r AC•T 5diff Rinse reagent is sometimes referred to as Rinse.
r AC•T 5diff Fix reagent is sometimes referred to as Fix.
r AC•T 5diff Hgb Lyse reagent is sometimes referred to as Hgb Lyse.
r AC•T 5diff WBC Lyse reagent is sometimes referred to as WBC Lyse.
r AC•T 5diff Diluent reagent is sometimes referred to as Diluent or diluent.
r In the electronic version of the manual:
t Links to additional information are in blue and are underlined. To access the linked
information, select the blue underlined text.
t The material is divided into many small sections (electronic files) to enhance the
loading and accessibility features.
t Every primary heading is a separate file and whenever possible the amount of
material contained within one primary heading is limited to four to ten pages.
t If a primary heading must be large, such as an illustrated parts list (IPL), invisible
breaks are added to the electronic file to further divide it.
Note: Unless you are scrolling, these divisions are invisible. If you choose to scroll
through the IPL, you will encounter stop points. When you scroll to the end of a
section and encounter a stop point, use the navigation bar to access the next
section.
t To move from one section (electronic file) to the next in the HTML version of the
manual, use the right and left arrows on the navigation bars displayed at the top and
bottom of each section.
Graphics
All graphics, including screens and printouts, are for illustration purposes only and must not
be used for any other purpose.
1.1-4 PN 4237616B
INTRODUCTION
SAFETY PRECAUTIONS 1
1.2 SAFETY PRECAUTIONS
Electronic
WARNING Risk of personal injury. Contacting exposed electronic components while the instrument is
attached to power can cause personal injury from electric shock. Power down completely before removing
covers to access electronic components.
WARNING Risk of personal injury or damage to electronic components. While performing maintenance or
service on the instrument, rings and other metal jewelry can become caught in the instrument. To avoid
personal injury or damage to the instrument, remove rings and other metal jewelry before performing
maintenance or service on the electronic components of the instrument.
CAUTION Risk of damage to electronic components. If the power is ON while removing or replacing printed
circuit cards and components, the instrument could be damaged. To prevent damage to electronic
components, always be sure power is OFF before removing or replacing printed circuit cards and
components.
CAUTION Risk of damage to electronic components. Electrostatic discharge (ESD) can damage add-in
circuit cards and other electronic components. If there is a possibility of ESD damage with a procedure,
then perform that procedure at an ESD workstation, or wear an antistatic wrist strap attached to a metal part
of the chassis connected to an earth ground.
Biological
WARNING Risk of personal injury or contamination. If you do not properly shield yourself while servicing
the instrument with the doors open, you may become injured or contaminated. To prevent possible injury or
biological contamination, you must wear appropriate safety glasses, a lab coat, and gloves when servicing
the instrument with the doors open.
Use care when working with pathogenic materials. Means must be available to decontaminate
the instrument, provide ventilation, and to dispose of waste liquid. Refer to the following
publications for further guidance on decontamination:
r Biohazards Safety Guide, 1974, National Institute of Health.

r Classifications of Etiological Agents on the Basis of Hazards, 3d ed., June 1974, Center
for Disease Control, U.S. Public Health Service.
Troubleshooting
Bring the following Warning to the customer’s attention before advising that customer to
perform any service, maintenance or troubleshooting procedures on the AC•T 5diff
hematology analyzer. Also, make sure customers are aware of the Warning and information
labels shown in Figure 1.2-1.
PN 4237616B 1.2-1
INTRODUCTION
SAFETY PRECAUTIONS
WARNING Risk of personal injury or contamination. If you do not properly shield yourself while performing
service, maintenance, and troubleshooting procedures, residual fluids in the instrument could injure or
contaminate you. Beckman Coulter recommends that you wear barrier protection, such as appropriate
safety glasses, a lab coat, and gloves throughout the performance of service, maintenance, and
troubleshooting procedures to avoid contact with cleaners and residual fluids in the instrument.
Figure 1.2-1 Warning and Information Label
M O D c
M
N O
O
A S S Y
N O .
.
D
x x x x x x S /N
x x x x x x
N O . A •T 5 d i f f
1 0 0 -2 4 0 5 0 /6 0
V O L T S H Z A M P S W A T T S
MANUFACTURED BY COULTER CORPOR ATION
BECKMAN 11800 A BECKM AN COULTER COMPANY
COULTER SW 147 AVEN UE, MIAMI, FLOR IDA 33196-2500 U.S.A.
PATTEN TS ISSUED AND/OR PEN DING
A U T O M A T E D D IF F E R E N T IA L C E L L C O U N T E R
A S S Y
x x x x x x x x x x x x
F O R IN V IT R O D IA G N O S T IC U S E
C A U T IO N :
S /N
N O .
T O R E D U C E T H E R IS K O F E L E C T R IC A L S H O C K D O N O T R E M O V E T H E C O V E R
O R B A C K .
R E F E R S E R V I C IN G T O Q U A L I F I E D S E R V IC E P E R S O N N E L .
E L E C T R IC S H O C K H A Z A R D . D I S C O N N E C T U N IT F R O M P O W E R S O U R C E
P R IO R T O S E R V I C I N G .
F O R C O N T I N U E D P R O T E C T I O N A G A I N S T R F IR E H A Z A R D , R E P L A C E O N L Y
W I T H S A M E T Y P E A N D R A T IN G O F F U S E .
F O R S A F E T Y R E A S O N S , E Q U IP M E N T R E Q U I4 R E S C O N N E C T IO N T O
P R O T E C T IV E E A R T H G R O U N D .
1 0 0 -2 4 0 5 0 -6 0 0 .9 -2 .0 2 0 0
MANUFACTURED FOR BECKMAN COULTER INC.
BECKMAN 11800 SW 147 AVENUE, MIAMI, FLORIDA 33196-2500 U.S.A.
PATENTS ISSUED AND/OR PENDING
COULTER MADE IN FRANCE
C A U T IO N :
O R B A C K .
R E F E R S E R V IC IN G T O Q U A L IF IE D S E R V IC E P E R S O N N E L .
E L E C T R IC S H O C K H A Z A R D . D IS C O N N E C T U N IT F R O M P O W E R S O U R C E
P R IO R T O S E R V IC IN G .
F O R C O N T IN U E D P R O T E C T IO N A G A IN S T R F IR E H A Z A R D , R E P L A C E O N L Y
W IT H S A M E T Y P E A N D R A T IN G O F F U S E .
F O R S A F E T Y R E A S O N S , E Q U IP M E N T R E Q U IR E S C O N N E C T IO N T O
T H IS A R E A M A Y C O N T A IN
B IO H A Z A R D O U S M A T E R IA L
R E F E R T O P R O D U IC T R E F E R E N C E
M A N U A L F O R P R O P E R H A N D L IN G
C A U T IO N
A L L C O V E R S /P A N E L S M U S T B E
S E C U R E D IN P L A C E P R IO R T O
IN S T R U M E N T O P E R A T IO N .
R E F E R T O P R O D U C T R E F E R E N C E
M A N U A L F O R P R O P E R IN S T A L L A T IO N .
2 4 2 9 5 5 5
1.2-2 PN 4237616B
CONTENTS
2 INSTRUMENT DESCRIPTION, 2.1-1
2.1 INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER, 2.1-1

Purpose, 2.1-1
Function, 2.1-1
Description, 2.1-1
Components, 2.1-1
Interaction with the AC•T 5diff Hematology Analyzer, 2.1-1
Modes of Operation, 2.1-3
CBC Mode, 2.1-3
CBC/DIFF Mode, 2.1-3
Reagent Consumption, 2.1-4
2.2 OPERATION PRINCIPLES, 2.2-1

Overview, 2.2-1
Measurement Principles, 2.2-1
Coulter Principle, 2.2-1
Aperture Sensor System, 2.2-1
Applying the Coulter Principle, 2.2-2
C
A V Technology, 2.2-3
Dual Focused Flow (DFF), 2.2-3
Flow Cell, 2.2-3
Focused Flow Impedance, 2.2-4
Absorbance Cytochemistry, 2.2-4
Signal Processing, 2.2-4
Thresholds, 2.2-4
WBC/BASO Methodology, 2.2-5
Sample Analysis Overview, 2.2-5
Aspiration, 2.2-5
Dilution, 2.2-6
Delivery, 2.2-6
Sample Partitioning, 2.2-7
2.3 CYCLE DESCRIPTION, 2.3-1

Cycle Start Conditions, 2.3-1
Sample Flow, 2.3-2
2.4 SAMPLE ANALYSIS, 2.4-1

RBC and Platelet Analysis, 2.4-1
Parameter Results Obtained from the RBC/Plt Dilution, 2.4-2
Hgb Measurement, 2.4-3
WBC Count and Differential, 2.4-4
Parameter Results Obtained from the WBC/BASO Dilution, 2.4-5
Differential, 2.4-5
Parameter Results Obtained from the DIFF Dilution, 2.4-6
Dilution Summary, 2.4-7
PN 4237616B 2-i
CONTENTS
2.5 RBC PARAMETER DEVELOPMENT, 2.5-1

RBC Count, 2.5-1
RBC Histogram, 2.5-1
Parameter Results Obtained Using the RBC Histogram, 2.5-2
Hct Measurement, 2.5-2
MCV Calculation, 2.5-2
RDW Calculation, 2.5-2
RBC Distribution Flags, 2.5-2
RBC1 and RBC2 Thresholds, 2.5-3
Flags, 2.5-3
Hgb Determination, 2.5-3
Hgb Blank Reading, 2.5-3
Sample Reading, 2.5-4
Hgb Specific Flags, 2.5-4
MCH and MCHC Calculations, 2.5-4
MCH Calculation, 2.5-4
MCHC Calculation, 2.5-4
2.6 PLATELET PARAMETER DEVELOPMENT, 2.6-1

Plt Count, 2.6-1
Platelet Distribution Curve, 2.6-1
Parameter Results Obtained Using the Plt Histogram, 2.6-2
MPV Measurement, 2.6-2
Pct Calculation, 2.6-2
PDW Calculation, 2.6-2
Detecting Abnormal Platelet Distributions, 2.6-3
Identifying a Normal Distribution, 2.6-3
Interference on the Lower End of the Platelet Distribution Curve, 2.6-3
Microcytic Interferences on the Upper End of the Platelet Distribution
Curve, 2.6-3
Microcytic Interference with a Distinct Valley between 18 fL and 25 fL, 2.6-4
Microcytic Interference with a Valley below 18 fL, 2.6-4
Interference with No Distinct Valley, 2.6-5
2.7 WBC PARAMETER DEVELOPMENT, 2.7-1

Overview, 2.7-1
WBC/BASO Dilution, 2.7-1
WBC Count, 2.7-1
BASO Count, 2.7-1
DIFF Dilution, 2.7-2
DiffPlot Development, 2.7-2
DiffPlot Regions Defined, 2.7-3
Neutrophil (Neut), 2.7-3
Lymphocyte (Lymph), 2.7-3
Monocyte (Mono), 2.7-3
Eosinophil (Eos), 2.7-3
Debris, 2.7-3
2-ii PN 4237616B
CONTENTS
Immature White Blood Cells, 2.7-4

Immature Granulocytes, 2.7-4
Band Cells, 2.7-4
Blast Cells, 2.7-4
DiffPlot Thresholds, 2.7-4
2.8 PNEUMATIC/HYDRAULIC SYSTEM, 2.8-1

Functions of Valves, 2.8-1
Pneumatic Diagrams, 2.8-4
Diluter System, 2.8-11
Diluent Input (Figure 2.8-10), 2.8-11
5diff Syringe and Flow Cell, 2.8-12
Probe and Probe Rinse, 2.8-13
Diluent to Baths, 2.8-13
Waste System, 2.8-15
2.9 ELECTRONIC SYSTEM, 2.9-1

Plug/Jack Labels, 2.9-1
Optical Preamplifier Card, 2.9-1
LCD and Keypad Card, 2.9-1
LED Card, 2.9-1
Motor Interconnect Card, 2.9-1
Traverse Interconnect Card, 2.9-1
2.10 SOFTWARE STRUCTURE, 2.10-1

Overview, 2.10-1
Menu Trees, 2.10-1
How to Select a Menu Item, 2.10-1
ILLUSTRATIONS
2.1-1 User Interfaces on the AC•T 5diff Hematology Analyzer, 2.1-2
2.2-1 Coulter Principle, 2.2-2
2.2-2 Dual Focused Flow Process, 2.2-3
2.2-3 Signal Processing, 2.2-4
2.2-4 Basophil Thresholds, 2.2-5
2.2-6 Sample Delivery Using Tangential Flow, 2.2-6
2.2-7 CBC/DIFF Mode -
2.2-8 CBC Mode -
2.3-1 Sample Probe and LED at Start of a Cycle, 2.3-1
2.3-2 Baths Assembly at Start of a Cycle, 2.3-1
2.3-3 Rinsing Probe Exterior After Aspiration, 2.3-2
2.3-4 Making the RBC/PLT First Dilution, 2.3-2
2.3-5 Making the WBC/BASO Dilution, 2.3-3
2.3-6 Making the DIFF Bath Dilution, 2.3-3
2.3-7 Double Rinse of the Sample Probe, 2.3-4
2.3-8 Aspirating from the First Dilution, 2.3-4
2.3-9 Rinsing the Outside of the Probe, 2.3-5
PN 4237616B 2-iii
CONTENTS
2.3-10 Making the RBC/Plt Dilution, 2.3-5

2.4-3 Flow Cell Operation, 2.4-5
2.5-1 Typical RBC Histogram, 2.5-1
2.5-2 RBC1 and RBC2 Positions - RBC Histogram, 2.5-2
2.6-1 Typical Plt Histogram, 2.6-1
2.6-2 Area of the Plt Histogram Used to Determine the PDW Parameter Result, 2.6-2
2.6-3 Typical Platelet Distribution Curve, 2.6-3
2.6-4 Microcytic Interference with a Valley between 18 fL and 25 fL, 2.6-4
2.6-5 Microcytic Interference with a Valley below 18 fL, 2.6-4
2.6-6 Interference with no Distinct Valley, 2.6-5
2.7-1 Areas Used to Determine WBC and BASO Parameter Results, 2.7-1
2.7-3 Volume Thresholds, 2.7-5
2.7-4 Absorbance Thresholds / NL, NE and MN Alarms, 2.7-6
2.8-1 Valve 1 through Valve 16 Locations, 2.8-1
2.8-2 Valve 17 and 18 Location, 2.8-1
2.8-3 Valve 20 to Valve 31 Locations, 2.8-2
2.8-4 Hgb Lyse Reagent Circuit, 2.8-5
2.8-5 Fix Reagent Circuit, 2.8-6
2.8-6 WBC Lyse Reagent Circuit, 2.8-7
2.8-7 Rinse Reagent Supply Circuit, 2.8-8
2.8-8 Probe Rinse Reagent Circuit, 2.8-9
2.8-9 WBC/BASO Rinse Reagent Circuit, 2.8-10
2.8-10 Diluent Reagent Circuit, 2.8-11
2.8-11 Probe Diluent Reagent Circuit, 2.8-13
2.8-12 Bath Diluent Reagent Circuit, 2.8-14
2.8-13 Waste Circuit, 2.8-15
2.10-1 User Menu Tree, 2.10-2
2.10-2 Service Menu Tree, 2.10-3
TABLES
2.1-1 AC•T 5diff Hematology Analyzer Reagent Consumption, Software Version
1.03, 2.1-4
2.2-1 AC•T 5diff Analyzer Measurement Technologies, 2.2-1
2.4-1 Technical Characteristics for Obtaining RBC and Platelet Counts, 2.4-2
2.4-2 Technical Characteristics for the Measurement of the Hemoglobin, 2.4-3
2.4-3 Characteristics Required to Obtain WBC and BASO Results, 2.4-4
2.4-4 Technical Characteristics for Acquisition of the DiffPlot, 2.4-6
2.4-5 Summary of Dilutions, 2.4-7
2.8-1 Valves and their Functions, 2.8-3
2-iv PN 4237616B
2INSTRUMENT DESCRIPTION 2
2.1 INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER
Purpose
The purpose of the AC•T 5diff hematology analyzer is to identify normal patient results with
all normal system-generated parameters and to flag or identify patient results that require
additional studies.
Function
The AC•T 5diff analyzer is a quantitative, fully automated (microprocessor controlled)
hematology analyzer and leukocyte differential counter For In Vitro Diagnostic Use in
clinical laboratories. The AC•T 5diff hematology analyzer reports a complete blood count
(CBC) and white blood cell differential (DIFF) on open-vial, whole-blood specimens.
The CBC consists of white blood cell count (WBC), red blood cell count (RBC), hemoglobin
(Hgb), hematocrit (Hct), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell
hemoglobin concentration (MCHC), red cell distribution width (RDW), platelet count (Plt),
and mean platelet volume (MPV).
The DIFF (a 5-part leukocyte differential) consists of the percentage (%) and absolute
number (#) of the following WBC populations: neutrophils (NE% and NE#), lymphocytes
(LY% and LY#), monocytes (MO% and MO#), eosinophils (EO% and EO#), and basophils
(BA% and BA#).
Six parameters are qualitative and are For Research Use Only. Not For In Vitro Diagnostic
Procedures. These parameters include the plateletcrit (Pct), platelet distribution width
(PDW), percentage and absolute number of immature cells (IMM% and IMM#), and
percentage and absolute number of atypical lymphocytes (ATL% and ATL#).
Description
Components
The AC•T 5diff hematology analyzer is contained in one unit, with additional space needed
only for the external printer, the diluent reagent container, and a waste container (if used).
Interaction with the AC•T 5diff Hematology Analyzer

The AC•T 5diff analyzer uses an Open-Vial mode of operation. Pressing the aspirate switch
(Figure 2.1-1) initiates a cycle. When the sample probe is submerged in a whole-blood
specimen and the aspirate switch is pressed, sample is pulled from the specimen tube into the
sample probe. As the cycle continues, the instrument then dilutes and analyzes this sample.
When the analysis is complete, results appear on a LCD (Figure 2.1-1) and are available to
the printer.
PN 4237616B 2.1-1
INSTRUMENT DESCRIPTION
INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER
Figure 2.1-1 User Interfaces on the AC•T 5diff Hematology Analyzer
L C D
K e y p a d
A s p ir a te
s w itc h
7 6 1 6 0 9 4 A
You also interact with the instrument through the use of a menu system displayed on a 128 by
240 pixels LCD and a control panel keypad with buttons that are used to setup and operate
the instrument (Figure 2.1-1).
Since most input/output functions of the operating system software are controlled by the user,
the pushbutton keypad and LCD screen are particularly important because they provide the
physical user interface with the software.
See Heading 2.10, SOFTWARE STRUCTURE for more specific information as well as a
graphic representation of the available menus and menu items (or options).
2.1-2 PN 4237616B
INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER 2
Modes of Operation
The AC•T 5diff hematology analyzer has two operating modes: CBC and CBC/DIFF.
CBC Mode
Twelve parameters are generated in the CBC mode of operation - 10 parameters For In Vitro
Diagnostic Use and two qualitative parameters that are For Research Use Only. Not For In
Vitro Diagnostic Procedures:
Parameters Parameters
(For In Vitro Diagnostic Use) (For Research Use Only)
WBC Pct
RBC PDW
Hgb
Hct
MCV
MCH
MCHC
RDW
Plt
MPV
CBC/DIFF Mode
26 parameters are generated in the CBC/DIFF mode of operation - 20 parameters For In Vitro
Diagnostic Use and six qualitative parameters that are For Research Use Only. Not For In
Vitro Diagnostic Procedures:
Parameters Parameters
(For In Vitro Diagnostic Use) (For Research Use Only)
WBC Pct
RBC PDW
Hgb IMM% and IMM#
Hct ATL% and ATL#
MCV
MCH
MCHC
RDW
Plt
MPV
NE% and NE#
LY% and LY#
MO% and MO#
EO% and EO#
BA% and BA#
PN 4237616B 2.1-3
INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER
Reagent Consumption
Table 2.1-1 shows the instrument reagent consumption by cycle.
Table 2.1-1 AC•T 5diff Hematology Analyzer Reagent Consumption, Software Version 1.03
Cycle AC•T 5diff reagents with usage per cycle Approximate Duration
Diluent WBC Lyse Rinse Fix Hgb Lyse

CBC 20.5 mL 2.1 mL 0.9 mL Not used 0.4 mL 1 minute
CBC/DIFF 25.6 mL 2.1 mL 0.9 mL 1.0 mL 0.4 mL 1 minute
Startup† 62.0 mL 2.1 mL 3.7 mL 1.0 mL 1.4 mL 3 minutes 40 seconds
Shutdown 25.5 mL Not used 14.0 mL Not used 1.0 mL 2 minutes 45 seconds
Prime diluent 35.5 mL Not used Not used Not used Not used 2 minutes 30 seconds
Prime rinse Not used Not used 25.8 mL Not used Not used 1 minute 20 seconds
Prime fix Not used Not used Not used 25.8 mL Not used 1 minute 30 seconds
Prime WBC Lyse Not used 25.8 mL Not used Not used Not used 1 minute 20 seconds
Prime Hgb Lyse 2.5 mL Not used Not used Not used 4.2 mL 1 minute
Prime All Reagents 23.7 mL 16.0 mL 16.0 mL 16.0 mL 4.2 mL 3 minutes 20 seconds
Extended Cleaning 12.5 mL Not used 6.0 mL Not used Not used 1 minute 35 seconds
System Reset Cycle 24.0 mL Not used 1.4 mL Not used 1.0 mL 1 minute 25 seconds
† For one background count only. The maximum is three.
2.1-4 PN 4237616B
OPERATION PRINCIPLES 2
2.2 OPERATION PRINCIPLES
Overview
The AC•T 5diff analyzer is a fully automated hematology analyzer providing a complete WBC
five-part differential, which is determined simultaneously by the ACV Technology
(Absorbance Cytochemistry and Volume Technology) and the white blood cell / basophil
(WBC/BASO) methodologies.
The ACV Technology uses absorbance, cytochemistry, and focused flow impedance. The
WBC/BASO methodology uses differential lysis, impedance technology, and differential
thresholds. See Table 2.2-1.
me
Table 2.2-1 AC•T 5diff Analyzer Measurement Technologies
Fluid Dynamics Technology Measurements Output

Dual Focused Flow ACV Technology Light absorbance of Lymphocytes, monocytes,
cytochemically-stained neutrophils, eosinophils,
cells immature cells, and
atypical lymphocytes
Volume aperture Differential lysis using the Volume and count WBC count, basophil
Coulter Principle percentage, and basophil
count
Volume aperture Coulter Principle Volume and count RBC count, platelet count,
and hematocrit
Measurement Principles
Coulter Principle
In the AC•T 5diff analyzer, the Coulter Principle is used to analyze the final red blood cell and
platelet (RBC/Plt) dilution and the WBC/BASO dilution. This electronic method of counting
and sizing particles is based on the fact that cells, which are poor conductors of electricity,
will interrupt a current flow. The impedance variation generated by the passage of
nonconductive cells through a small, calibrated aperture is used to determine the count
(number of particles) and size (volume) of the particles passing through the aperture within a
given time period.
Aperture Sensor System

The RBC/Plt aperture sensor system determines the cell count and size of red blood cells and
platelets. The WBC/BASO aperture sensor system determines the cell count and size of white
blood cells. Additionally, the differentiation between basophils and other white blood cells is
related to the AC•T 5diff WBC Lyse-specific lytic action on the white blood cells in the
WBC/BASO bath.
PN 4237616B 2.2-1
OPERATION PRINCIPLES
To sense particles using the Coulter Principle (Figure 2.2-1), a current flow is established so
changes in that flow can be monitored. In this sensing system, an electrode is placed on each
side of the aperture (Figure 2.2-1). The most visible electrode is referred to as the counting
head. These electrodes are the conductive metallic housings attached to the front of the RBC
and WBC/BASO baths. The second electrode, referred to as the bath electrode, is not as
conspicuous. This electrode is located inside the bath. The aperture is located between the
counting head and the bath electrode.
Figure 2.2-1 Coulter Principle

Solution to be analyzed
Vacuum
constant
Constant
current
Volts
Analyzing
Electrodes electronic
circuit
Pulse Time 7616035A
When the count circuit is activated and an electronically conductive reagent is in the RBC or
WBC/BASO bath, an electric current continuously passes through the aperture. Current
moving between the two electrodes establishes the electronic flow through the aperture.
Once a sample is aspirated, an aliquot of that aspirated sample is diluted with reagent (an
electrolyte) and is delivered to the RBC or WBC/BASO bath using tangential flow, which
ensures proper mixing of the dilution. When the cells suspended in the conductive reagent
are pulled through a calibrated aperture, the electrical resistance between the two electrodes
increases proportionately with the cell volume (Figure 2.2-1).
The resistance creates a pulse that is sensed and counted as a particle by the instrument. The
amount of resistance (amplitude of each pulse) is directly related to the size of the particle
that produced it.
The generated pulses have a very low voltage, which the amplification circuit increases so
that the electronic system can better analyze the pulses and eliminate the background noise.
Applying the Coulter Principle

The AC•T 5diff analyzer makes several dilutions of an aspirated whole-blood sample. The
RBC/Plt dilution begins in the DIL1/HGB (first dilution/hemoglobin) bath but is actually
analyzed in the RBC bath. The final dilution in the RBC bath is used to determine the cell
count and size of red blood cells and platelets.
The WBC/BASO aperture sensor system is directly responsible for determining the cell count
and size of white blood cells. The differentiation between basophils and other white blood
cells is also related to the AC•T 5diff WBC Lyse-specific lytic action on these white blood
cells.
2.2-2 PN 4237616B
Thresholds, which are electronically set size limits, exclude unwanted particles, such as
debris, from the analysis. Particles above the threshold are analyzed, and particles below the
threshold are excluded.
ACV Technology
In the DIFF (differential) bath, 25 µL of whole blood is mixed with 1,000 µL of AC•T 5diff
Fix reagent for 12 seconds, then stabilized with 1,000 µL of AC•T 5diff Diluent for an
additional three seconds. This reaction lyses the red blood cells, preserves the leukocytes at
their original size, and differentially stains the lymphocytes, monocytes, neutrophils, and
eosinophils, with eosinophils staining most intensely. The instrument maintains the reagents
and reaction at a regulated temperature of 35°C (95°F).
Lymphocytes, monocytes, neutrophils, and eosinophils each have a unique nuclear and
morphology structure and staining intensity; therefore, each cell type absorbs light differently.
Each stained cell is individually focused by the Dual Focused Flow (DFF) system and
transported through the flow cell using sample pressure and diluent sheath flow.
Dual Focused Flow (DFF)

DFF fluid dynamics uses a hydrodynamic focusing process to focus individual cells or
particles in a stream of diluent (Figure 2.2-2). The focused sample stream of the AC•T 5diff
analyzer is about 40 µm in diameter.
Figure 2.2-2 Dual Focused Flow Process
DFF uses sheath fluid to surround and force cells suspended in diluent to pass one at a time
through the center of the flow cell. The first sheath flow focuses the sample through the
impedance aperture. The second sheath flow maintains the focused flow of cells as they exit
the aperture into the optical flow cell. Hydrodynamic focusing in the flow cell enables
accurate and rapid cell-by-cell measurements on a large number of individual cells.
Flow Cell
Sequential analyses for cell volume (impedance) and light absorbance are performed in the
flow cell. A total of 72 µL of sample is injected through the flow cell for 15 seconds. The flow
cell incorporates a 60 µm aperture for cellular volume analysis and about a 40 µm
measurement area for light absorbance.
PN 4237616B 2.2-3
Focused Flow Impedance

Focused flow impedance technology measures the electrical resistance of a cell as it passes
through the aperture in the flow cell. The change in resistance is directly proportional to the
volume of the cell.
Absorbance Cytochemistry
As a cell passes through the optical portion of the flow cell, light is scattered in all directions.
A sensor detects only forward scattered light. The optical measurement is derived as a
function of the amount of light lost due to diffraction and absorbance, as compared to full
transmission when no cell is present.
The collected signals are converted into voltage pulses and are processed. The magnitude of
the voltage pulses are proportional to the physical and chemical characteristics of the cells
being analyzed. Light absorbance is related to cellular contents (granularity, nuclear content,
and so forth) after cytochemical staining. These measurements provide the information for
lymphocytes, monocytes, neutrophils, and eosinophils, and their precursors.
Signal Processing
The signals from the flow cell aperture and from the optical measurement are correlated by a
window of time. The optical pulse must be detected within 100 to 300 microseconds of the
impedance pulse; otherwise, the signal is rejected.
The output signals from the focused flow impedance and the light absorbance measurements
are combined to define the WBC differential population clusters. See Figure 2.2-3.
Figure 2.2-3 Signal Processing
Thresholds
Most of the population partition thresholds are fixed and give the limits of the morphological
normality of leukocytes. Changes in the morphology of a population are expressed on the
DiffPlot by a shifting of the corresponding population. Volume and absorbance thresholds are
used to detect shifting populations.
2.2-4 PN 4237616B
WBC/BASO Methodology
In the WBC/BASO bath, 10 µL of whole blood is mixed with 2,000 µL of AC•T 5diff WBC
Lyse reagent. This reaction lyses the red blood cells and specifically differentiates between
basophils and other leukocytes by volume. The instrument maintains the reagents and
reaction at a regulated temperature of 35°C (95°F).
Using a constant vacuum, the instrument then pulls the sample through an 80 µm aperture.
As each cell passes through the aperture, a pulse is generated proportional to the cellular
volume. The total leukocyte count and basophil percentage are determined by specific
thresholds on the WBC/BASO histogram (Figure 2.2-4).
Figure 2.2-4 Basophil Thresholds
Sample Analysis Overview
Aspiration
When the sample probe is immersed in a whole-blood specimen and the aspirate switch is
pressed, sample is pulled from the tube into the sample probe. Depending on the selected
mode of operation, the AC•T 5diff analyzer aspirates either 30 µL (CBC mode) or 53 µL
(CBC/DIFF mode) of sample.
The volume of sample aspirated into the sample probe is sufficient to make all the dilutions
needed to develop parameter results in the selected mode of operation.
PN 4237616B 2.2-5
Dilution
Using the Sequential Dilution System (SDS) technique, the aspirated sample is partitioned as
it is distributed to make a series of dilutions in a series of baths (Figure 2.2-5).
Figure 2.2-5 Bath Assembly
Delivery
In the CBC and the CBC/DIFF modes, each aliquotted sample is delivered to its appropriate
bath using a tangential flow (Figure 2.2-6) of reagent, which mixes the diluted sample and
minimizes viscosity problems.
Figure 2.2-6 Sample Delivery Using Tangential Flow
Probe
Reagent
input Tangential flow
Bath 7616002A
2.2-6 PN 4237616B
Sample Partitioning
Figure 2.2-7 shows the sample partitioning that occurs in the CBC/DIFF mode. Notice there
are three aliquots of the aspirated whole-blood sample that will be used to make dilutions.
Figure 2.2-7 CBC/DIFF Mode - CBC/DIFF Mode

Sample Partitions inside the Probe
After aspiration in the CBC/DIFF mode, aliquots of
the whole-blood sample are distributed to the
Diluent
various baths as follows (Figure 2.2-5):
r The 3 µL sample aliquot at the tip of the probe

Air bubble is discarded into the rinse chamber as the
exterior of the sample probe is rinsed,
ensuring sample integrity.
Not used
r 10 µL of sample is delivered to the DIL1/HGB
bath for use in preparing the primary RBC/Plt
DIFF dilution
dilution and for measuring the Hgb value.
r 10 µL of sample is delivered to the WBC/BASO
WBC/BASO dilution bath for the WBC/BASO count.
r 25 µL of sample is delivered to the DIFF bath
RBC/PLT/HGB first dilution
for development of the DiffPlot.
Not used r 5 µL of remaining sample is discarded into the
7616001A rinse chamber.
Figure 2.2-8 shows the sample partitioning that occurs in the CBC mode. Notice there are
only two aliquots of the aspirated whole-blood sample that will be used to make dilutions in
this mode of operation. (The DIFF aliquot is not needed in the CBC mode.)
Figure 2.2-8 CBC Mode - CBC Mode

Sample Partitions inside the Probe
After aspiration in the CBC mode, aliquots of the
whole-blood sample are distributed to the various
baths as follows (Figure 2.2-5):
r The 3 µL sample aliquot at the tip of the probe

Diluent is discarded into the rinse chamber as the
exterior of the sample probe is rinsed,
ensuring sample integrity.
Air bubble
r 10 µL of sample is delivered to the DIL1/HGB
bath for use in preparing the primary RBC/Plt
Not used dilution and for measuring the Hgb value.
r 10 µL of sample is delivered to the WBC/BASO
WBC/BASO dilution bath for the WBC/BASO count.
r 7 µL of remaining sample is discarded into the
RBC/PLT/HGB first dilution rinse chamber.
Not used
7616056A
PN 4237616B 2.2-7
2.2-8 PN 4237616B
CYCLE DESCRIPTION 2
2.3 CYCLE DESCRIPTION
This cycle description focuses on the sequence of the sample probe movement among the
baths. It also focuses on the volume of sample and reagents being delivered to make the
dilutions needed for sample analysis.
Cycle Start Conditions
Figure 2.3-1 Sample Probe and LED at Start of a Cycle r The sample probe is in its home position.
r The green LED is glowing indicating the
instrument is ready.
Figure 2.3-2 Baths Assembly at Start of a Cycle r All the baths (except the rinse chamber) are
filled with clean diluent.
PN 4237616B 2.3-1
CYCLE DESCRIPTION
Sample Flow
r To initiate a cycle, submerge the sample probe in

a well-mixed whole-blood specimen and press
the aspirate switch to start the cycle.
r All the baths drain.
r A sample of the whole-blood specimen is
aspirated.
t 53 µL in the CBC/DIFF mode.
t 30 µL in the CBC mode.
Figure 2.3-3 Rinsing Probe Exterior After Aspiration r The horizontal traverse assembly positions the
sample probe over the rinse chamber.
r 3 µL sample aliquot at the tip of the sample
probe is discarded into the rinse chamber as the
exterior of the sample probe is rinsed.
Discarding this aliquot helps ensure sample
integrity.
Figure 2.3-4 Making the RBC/PLT First Dilution r The horizontal traverse assembly positions the
sample probe over the DIL1/HGB (first
dilution/Hgb) bath.
r The vertical traverse assembly moves the probe
downward into the bath. The probe tip is
positioned to produce a tangential flow when the
sample and diluent are simultaneously dispensed
into the bath. For a more detailed description of
tangential flow, see Delivery under Heading 2.2,
OPERATION PRINCIPLES.
r 10 µL of the whole-blood partitioned for making
the first dilution is delivered to the DIL1/HGB
bath using a tangential flow of 1.7 mL of diluent.
r The tangential flow of reagent mixes the sample
and the diluent. Mixing bubbles enter the bath to
make a uniform suspension of cells. This 1:170
dilution is commonly referred to as the first
dilution.
2.3-2 PN 4237616B
CYCLE DESCRIPTION 2
Figure 2.3-5 Making the WBC/BASO Dilution r The horizontal traverse assembly positions the
sample probe over the WBC/BASO bath
r The vertical traverse moves the probe downward
into the bath. The tip of the probe is positioned
so that a tangential flow occurs as the 10 µL of
the whole-blood sample and 2.0 mL of WBC
Lyse are simultaneously dispensed into the bath.
and reagent. Mixing bubbles enter the bath to
make a uniform suspension of cells. The WBC
Lyse destroys the red blood cells and the specific
lytic action on the white blood cells
differentiates the basophils from other WBCs.
WBC/BASO Bath Dilution

Whole-blood volume 10 µL
Volume of AC•T 5diff WBC Lyse reagent 2000 µL
Dilution ratio 1:200
Figure 2.3-6 Making the DIFF Bath Dilution r The horizontal traverse assembly moves the
sample probe over the DIFF bath.
downward into the bath.
r The tip of the probe is positioned so that a
tangential flow occurs as 25 µL of the
whole-blood sample and 1.0 mL of Fix reagent
are simultaneously dispensed into the bath.
and the Fix reagent. Mixing bubbles enter the
bath to make a uniform suspension of cells. The
Fix reagent lyses the red blood cells, stabilizes
the WBCs in their native form, and differentially
stains the lymphocytes, monocytes, neutrophils,
and eosinophils, with the eosinophils staining
most intensely.
r After 12 seconds of incubation, the staining
process inside the DIFF bath is completed by
adding another 1.0 mL of diluent which stops
the cytochemical reaction.
DIFF Bath Dilution

Whole-blood volume 25 µL
Volume of AC•T 5diff Fix reagent 1000 µL
Volume of AC•T 5diff Diluent 1000 µL
Final dilution ratio 1:80
PN 4237616B 2.3-3
CYCLE DESCRIPTION
Figure 2.3-7 Double Rinse of the Sample Probe r The horizontal traverse assembly moves the
sample probe over the rinse chamber.
r A double rinsing (interior and exterior) of the
probe removes residual whole-blood sample
from inside the probe.
t In the CBC/DIFF mode, 5 µL is discarded in
the rinse chamber.
t In the CBC mode, 7 µL is discarded in the
rinse chamber.
Figure 2.3-8 Aspirating from the First Dilution r The horizontal traverse assembly moves the
sample probe over the DIL1/HGB bath.
r 42.5 µL of the 1:170 first dilution is aspirated
into the sample probe.
2.3-4 PN 4237616B
CYCLE DESCRIPTION 2
Figure 2.3-9 Rinsing the Outside of the Probe r While still inside the DIL1/HGB bath, the
exterior of the sample probe is rinsed with
0.4 mL of diluent.
up out of the bath.
r 0.4 mL of Hgb Lyse is added to the bath. The
Hgb Lyse reagent rapidly destroys the red blood
cells and converts a substantial proportion of the
hemoglobin to a stable pigment so a hemoglobin
value can be determined.
r Mixing bubbles enter the bath to ensure a
uniform dilution.
DIL1/HGB Bath Dilution

First dilution 1:170
Volume of first dilution removed 42.5 µL
Volume of AC•T 5diff Hgb Lyse reagent 400 µL
Volume of AC•T 5diff Diluent reagent 400 µL
Figure 2.3-10 Making the RBC/Plt Dilution r The horizontal traverse assembly moves the
sample probe over the RBC bath.
r The tip of the probe is positioned so that a
tangential flow occurs as the 42.5 µL of 1:170
dilution obtained from the first dilution in the
DIL1/HGB bath and 2.0 mL of diluent are
simultaneously dispensed into the bath
r An additional 0.5 mL of diluent is dispensed
through the probe at the end of the second
dilution.
RBC Bath Dilution

Volume 1:170 dilution from DIL1/HGB bath 42.5 µL
Volume of AC•T 5diff Diluent reagent 2500 µL
Final dilution ratio 1:10,000
PN 4237616B 2.3-5
CYCLE DESCRIPTION
2.3-6 PN 4237616B
SAMPLE ANALYSIS 2
2.4 SAMPLE ANALYSIS
RBC and Platelet Analysis
The RBC/Plt dilution analyzes red blood cells and platelets. This dilution is prepared in two
stages – the primary (first) dilution and the secondary (last) dilution.
The primary dilution is made in the DIL1/HGB bath, and the secondary dilution is made in
the RBC bath (Figure 2.4-1).
Table 2.4-1 summarizes the technical characteristics required to obtain RBC and Platelet
results.
PN 4237616B 2.4-1
SAMPLE ANALYSIS
Table 2.4-1 Technical Characteristics for Obtaining RBC and Platelet Counts
Dilution Characteristics
Primary Dilution for RBC and Plt:
Initial volume of whole-blood 10 µL
Volume AC•T 5diff diluent 1700 µL
Primary dilution ratio 1:170
Secondary Dilution for RBC and Plt:
Volume of primary dilution 42.5 µL
Secondary dilution ratio 1:58.8
Final dilution for RBC and Plt results 1:170 x 1:58.8 = 1:10,000
Reaction temperature 35°C (95°F)
Measurement Characteristics
Method of analysis Coulter Principle
Aperture diameter 50 µm
Count vacuum 200 mb (5.9 in. Hg)
Count period 2 x 5 seconds
Parameter Results Obtained from the RBC/Plt Dilution

This final 1:10,000 RBC/Plt dilution is used to:
r Determine the RBC count.
r Develop the RBC histogram, which is needed to obtain the Hct, MCV, and RDW results.
r Determine the Plt count.
r Develop the Plt histogram, which is needed to obtain the MPV, Pct, and PDW results.
2.4-2 PN 4237616B
SAMPLE ANALYSIS 2
Hgb Measurement
Hemoglobin is determined from the dilution in the DIL1/HGB bath (Figure 2.4-1). This
dilution is prepared in two stages – the primary (first) dilution and the secondary (last)
dilution.
The primary dilution is made and 42.5 µL of that dilution is removed for making the RBC/Plt
dilution. AC•T 5diff Hgb Lyse and additional diluent are added to make the final 1:250
dilution.
The Hgb concentration is based on the transmittance of light through the optical part of the
DIL1/HGB bath using a spectrophotometric technique at a wavelength of 550 nm. The
transmittance of the sample dilution is compared to the transmittance of a reagent blank. The
system calculates the Hgb using the blank and sample readings.
Table 2.4-2 summarizes the technical characteristics required for measuring hemoglobin.
.
Table 2.4-2 Technical Characteristics for the Measurement of the Hemoglobin
Volume of whole-blood 10 µL
Preliminary dilution ratio 1:170
Volume of the 1:170 dilution removed 42.5 µL
(for making the RBC/Plt dilution)
Additional volume of AC•T 5diff diluent 400 µL
Volume of AC•T 5diff Hgb Lyse 400 µL
Final dilution for Hgb determination 1:250
Method of analysis Spectrophotometry
Wavelength 550 nm
PN 4237616B 2.4-3
SAMPLE ANALYSIS
WBC Count and Differential

The WBC count is determined twice using two different methodologies:
r The reference WBC count is the count obtained in the WBC/BASO bath (Figure 2.4-2).
The WBC count and the BASO count are determined simultaneously.
r A second WBC count is determined in the flow cell during acquisition of the DiffPlot.
The dilution analyzed in the flow cell is prepared in the DIFF bath (Figure 2.4-2).
The WBC counts from the two methodologies are compared and if the results exceed the
predefined limits, they will be flagged.
Table 2.4-3 summarizes the technical characteristics required to obtain WBC and BASO
results.
Table 2.4-3 Characteristics Required to Obtain WBC and BASO Results
Volume AC•T 5diff WBC Lyse 2,000 µL
Dilution ratio 1:200
Method of analysis Coulter Principle
Count vacuum 200 mb (5.9 in. Hg)
Count period 2 x 6 seconds
2.4-4 PN 4237616B
SAMPLE ANALYSIS 2
Parameter Results Obtained from the WBC/BASO Dilution
The final 1:200 dilution is used to:
r Determine the WBC count, and
r Develop the WBC/BASO histogram, which is needed to obtain the BASO count.
Differential
Twenty-five microliters (25 µL) of whole blood is delivered to the DIFF bath in a flow of
AC•T 5diff Fix reagent, which lyses the red blood cells, stabilizes the WBC in their native
forms, and differentially stains the lymphocytes, monocytes, neutrophils, and eosinophils,
with the eosinophils staining most intensely.
The solution is then stabilized with diluent for three seconds and transferred to the
measuring bath. See Figure 2.4-3. Each cell is measured in absorbance (cytochemistry) and
resistivity (volume).
Figure 2.4-3 Flow Cell Operation

2) Second focused flow for optical detection
1) Primary focused flow for impedance
Table 2.4-4 summarizes the technical characteristics required for acquisition of the DiffPlot.
PN 4237616B 2.4-5
SAMPLE ANALYSIS
Table 2.4-4 Technical Characteristics for Acquisition of the DiffPlot
Volume AC•T 5diff Fix 1000 µL
Volume AC•T 5diff Diluent 1000 µL
Incubation duration 12 seconds
Method of analysis Impedance with hydrofocus
Diameter of the flow 42 µm
Volume injected 72 µL
Injection duration 15 seconds
Data accumulation 12 seconds
Parameter Results Obtained from the DIFF Dilution

From these measurements, a DiffPlot is developed with optical transmission (absorbance) on
the X-axis and volume on the Y-axis. Figure 2.4-4 shows the DiffPlot regions.
From the DiffPlot, four out of five leukocyte (white blood cell) populations are determined:
lymphocytes, monocytes, neutrophils, and eosinophils.
In a typical whole-blood sample, the basophil population (determined in the WBC/BASO

bath) is very small compared to the other four white blood cell populations.
Figure 2.4-4 DiffPlot Regions
2.4-6 PN 4237616B
SAMPLE ANALYSIS 2
Dilution Summary
Table 2.4-5 summarizes the dilution characteristics required to obtain CBC and CBC/DIFF
parameter results.
Table 2.4-5 Summary of Dilutions
Technical Characteristics Whole-Blood Reagent Reaction

for Volume Reagent(s) Volume Dilution Ratio Temperature
WBC Count and BASO count 10 µL AC•T 5diff WBC Lyse 2000 µL Final 35°C (95°F)
(in the WBC/BASO bath) 1:200
Differential Acquisition with 25 µL AC•T 5diff Fix 1000 µL Final 35°C (95°F)
Differential WBC Count 1000 µL 1:80
AC•T 5diff Diluent
(in the DIFF bath)
Hemoglobin Measurement 10 µL AC•T 5diff Diluent 1700 µL Preliminary 35°C (95°F)
(in the DIL1/HGB bath) 1:170
After removing 42.5 µL
of the 1:170 dilution:
AC•T 5diff Diluent 400 µL Final
400 µL 1:250
AC•T 5diff Hgb Lyse
RBC and PLT Count 42.5 µL of the AC•T 5diff Diluent 2500 µL Secondary 35°C (95°F)
(in the RBC bath) 1:170 dilution 1:58.8
Note: The primary dilution (from the 1:170 x 1:58.8 =
(1:170) is made in the DIL1/HGB bath)
Final
DIL1/HGB bath. 1:10,000
PN 4237616B 2.4-7
SAMPLE ANALYSIS
2.4-8 PN 4237616B
RBC PARAMETER DEVELOPMENT 2
2.5 RBC PARAMETER DEVELOPMENT
RBC/Plt Dilution
The final 1:10,000 dilution in the RBC bath contains red blood cells, white blood cells, and
platelets. Thresholds are used to separate the platelet pulses, which are much smaller, from
the red and white blood cell pulses. Since white blood cells fall in the red blood cell size
range, they are counted and sized as RBCs. The WBCs are not sorted out because any
interference is usually insignificant; there are normally very few WBCs (thousands) in
comparison to the number of RBCs (millions). Only when the white count is markedly
elevated is the red cell count or histogram influenced.
RBC Count
The AC•T 5diff hematology analyzer uses duplicate counting criteria, voting criteria, and
proprietary flagging information to confirm the parameter result prior to reporting it. To
obtain an RBC count result, the instrument compares the data from the two 5-second count
periods then votes and rejects any questionable data.
RBC count = Number of cells counted per volume unit x Calibration factor
The RBC count is displayed and printed as: RBC = N x 106 cells /µL.
Note: Cells per microliter (cells/µL) is the US reporting unit format. See Heading A.7 in the
Operator’s Guide for the other available formats.
RBC Histogram
In addition to being counted, red blood cells are categorized according to size (from 30 fL to
300 fL) by a 256-channel pulse-height analyzer. The pulse-height analyzer uses a number of
thresholds to sort the particles into several size (volume) categories and to develop a size
distribution curve of the particles. The RBC distribution curve shows cells in their native size.
Figure 2.5-1 is an example of an RBC histogram with a normal RBC size distribution.
Figure 2.5-1 Typical RBC Histogram
30 300
7616036A
PN 4237616B 2.5-1
RBC PARAMETER DEVELOPMENT
Parameter Results Obtained Using the RBC Histogram
Hct Measurement
The height of the pulse generated by the passage of a cell through the aperture is directly
proportional to the volume of the analyzed red blood cell. The hematocrit (Hct) is the sum of
all the digitized pulses. The Hct is displayed and printed as a percentage (%).
Note: Percentage (%) is the US reporting unit format. See Heading A.7 in the Operator’s
Guide for the other available formats.
MCV Calculation
The MCV (Mean Cell Volume) is calculated using the Hct and the RBC count. The MCV is
displayed and printed in femtoliters (fL).
Note: Femtoliters (fL) is the US reporting unit format. See Heading A.7 in the Operator’s
RDW Calculation
The RDW (Red cell Distribution Width) is an index of the variation or spread in the size of
the red blood cells. The study of the RBC distribution detects erythrocyte anomalies linked to
anisocytosis and enables the clinician to follow the evolution of the width of the curve
relative to the cell number and average volume. Displayed and printed as a percentage, RDW
is calculated using the standard deviation (SD) of the RBC population and the MCV.
K SD-
-------------- = RDW (%)
MCV
where:
K = System constant
SD = Calculated standard deviation based on the red cell distribution
MCV = Mean Cell Volume of the red cells
RBC Distribution Flags

Once the RBC distribution curve is developed, two positions on the distribution curve are
located (Figure 2.5-2):
Figure 2.5-2 RBC1 and RBC2 Positions - RBC Histogram

RBC1 RBC2
30 300
7616057A
2.5-2 PN 4237616B
RBC PARAMETER DEVELOPMENT 2
RBC1 and RBC2 Thresholds
Thresholds RBC1 and RBC2 define the MICRO and MACRO regions and are calculated based
on standard deviation (SD) of the RBC population.
The RBC1 threshold (monitoring area for microcytes) and the RBC2 threshold (monitoring
area for macrocytes) identify the points on the curve that are ±2 SD from the mean
(Figure 2.5-2).
Flags
Note: MICRO and MACRO flags will be activated in software version 1.0 and higher.
The MICRO flag is generated when the percentage of cells in the microcytic region compared
to the total number of RBCs exceeds the preset default limit of 5%. The MACRO flag is
generated when the percentage of cells in the macrocytic region compared to the total number
of RBCs exceeds the preset default limit of 7.5%. A laboratory may establish its own limits to
replace the preset default values.
Note: The MICRO and MACRO flags are independent of the Microcytosis and Macrocytosis
flags that are generated from the Low and High patient limits.
Hgb Determination
The hemoglobin (Hgb) released by the lysis of the red blood cells combines with the
potassium cyanide to form a stable cyanmethemoglobin compound.
This compound is measured through the optical part of the DIL1/HGB bath using a
spectrophotometric technique at a wavelength of 550 nm. Transmittance of the sample
dilution is compared with the transmittance of a reagent blank. The system calculates the Hgb
using both the blank and sample readings.
The final Hgb result in g/dL represents: absorbance value obtained x Calibration factor.
Hgb is displayed and printed as: Hgb = N g/dL.
Note: Grams per deciliter (g/dL) is the US reporting unit format. See Heading A.7 in the
Hgb Blank Reading

The Hgb blank value measured during the first patient cycle after a Startup cycle is stored as a
reference blank. This blank must be greater than 2.5 Vdc. During each analysis cycle, the
instrument checks the measured Hgb blank against the stored Hgb blank reference value
using the following formula:
(BlankRef x 1/3) + (BlankS x 2/3) = BlankNR

where:
BlankRef = Hgb blank reference value
BlankS = Hgb blank value from the current cycle
BlankNR = New Hgb blank reference value for comparison
Note: If the new Hgb blank reference value is within 3% of the old reference value,
the Hgb blank reference value is changed to this new value.
PN 4237616B 2.5-3
RBC PARAMETER DEVELOPMENT
Sample Reading
This value is based on the sample, diluent, and Hgb Lyse reagent mixture in the DIL1/HGB
bath during sample measurement.
Hgb Specific Flags

If the Hgb blank value is less than 2.5 Vdc, a reject (R) flag occurs on the Hgb value.
If the difference between the new Hgb blank reference value and the original Hgb blank
reference value is greater than 3%, a review (R) flag is generated. If three consecutive review
(R) flags occur on the Hgb blank reference value, the (. . . . .) code replaces the Hgb result.
For each Hgb sample read value, the instrument takes three readings. If the difference
between these readings exceeds the predefined limits (default setting is 60 A to D units), a
voteout (V) flag is generated.
MCH and MCHC Calculations
MCH Calculation
The MCH (Mean Cell Hemoglobin) is calculated from the Hgb value and the RBC count and
describes the average weight of hemoglobin in a red cell. The calculation for MCH is:
Hgb-
----------- × 10 = MCH (pg)
RBC
Note: Picograms (pg) is the US reporting unit format. See Heading A.7 in the Operator’s
MCHC Calculation
The MCHC (Mean Cell Hemoglobin Concentration) is calculated using the Hgb and Hct
values and describes the average concentration of hemoglobin in the red blood cells. The
calculation for MCHC is:
Hgb
---------- × 100 = MCHC (g/dL)
Hct
Note: Grams per deciliter (g/dL) is the US reporting unit format. See Heading A.7 in the
2.5-4 PN 4237616B
PLATELET PARAMETER DEVELOPMENT 2
2.6 PLATELET PARAMETER DEVELOPMENT
RBC/Plt Dilution
Platelet counting and sizing is also done in the RBC bath. Thresholds separate the platelet
pulses, which are much smaller, from the red and white blood cell pulses.
Plt Count
obtain a Plt count result, the instrument compares the data from the two 5-second count
periods then votes and rejects any questionable data.
Plt count = Number of cells counted per volume unit x Calibration factor.
The Plt count is displayed and printed as: Plt = N x 103 cells /µL.
Platelet Distribution Curve

Platelets are categorized according to size by a 256-channel pulse-height analyzer. A
pulse-height analyzer uses a number of thresholds to sort the particles into several size
(volume) categories and to develop a size distribution curve of the particles between 2 fL and
30 fL. The Plt distribution curve shows cells in their native size. Figure 2.6-1 is an example of
a Plt histogram with a normal Plt size distribution.
Figure 2.6-1 Typical Plt Histogram
3 25fL 30
PN 4237616B 2.6-1
PLATELET PARAMETER DEVELOPMENT
Parameter Results Obtained Using the Plt Histogram
MPV Measurement
The MPV (Mean Platelet Volume) is measured directly from analysis of the platelet
distribution curve. The MPV is displayed and printed in femtoliters (fL).
Note: Femtoliters (fL) is the US reporting unit format. See Heading A.7 in the Operator’s
Guide for the other available formats
Pct Calculation
The Pct (plateletcrit or thrombocrit) is calculated according to the formula:
3
Plt ( 10 /µL ) × MPV (fL)
--------------------------------------------------------------- = Pct%
10, 000
The Pct parameter result is displayed and printed as a percentage (%).
PDW Calculation
PDW (Platelet Distribution Width) is calculated from the Plt histogram as the width of the
curve between S1 and S2.
As shown in Figure 2.6-2, S1 and S2 are placed so that:
r 15% of the platelets occur between 2 fL and S1.

r 15% of the platelets occur between S2 and the variable upper threshold.
Note: This threshold is explained under the Detecting Abnormal Platelet Distributions
heading that follows.
r The PDW result is determined on the platelets between S1 and S2.
Figure 2.6-2 Area of the Plt Histogram Used to Determine the PDW Parameter Result
15%
15%
PDW
S1 S2
7615002A
The PDW parameter result is displayed and printed as a percentage (%).
2.6-2 PN 4237616B
Detecting Abnormal Platelet Distributions
Particles of approximately platelet size can interfere with the platelet histogram and count.
Small particles, such as microbubbles or dust, can overlap the low end. Microcytic red cells
can intrude at the upper end.
Identifying a Normal Distribution

When a platelet histogram is being evaluated, a mobile threshold can move from its starting
position at 25 fL to 18 fL (Figure 2.6-3). The computer searches for a valley between the
platelet and red cell populations. If no valley is detected between 18 fL and 25 fL, the
threshold remains at 25 fL and no flag is generated.
Figure 2.6-3 Typical Platelet Distribution Curve
3 25fL 30
Interference on the Lower End of the Platelet Distribution Curve

Particles that are approximately platelet size can interfere with the platelet histogram and
count. Small particles, such as microbubbles or dust, can interfere at the low end. If the
number of pulses in the 2 to 3 fL region is higher than the predefined limits, an SCL flag
appears to alert the operator that a significant number of small cells or interference, such as
microbubbles, are present.
Microcytic Interferences on the Upper End of the Platelet Distribution Curve

Microcytic red cells can intrude at the upper end of the platelet distribution curve. If the
specimen contains microcytes, the AC•T 5diff analyzer may be able to successfully eliminate
the influence of this interference by repositioning the variable threshold (25 fL threshold)
and excluding the microcytes.
PN 4237616B 2.6-3
Microcytic Interference with a Distinct Valley between 18 fL and 25 fL

If the intrusion of microcytes creates a valley between the 25 fL and the 18 fL thresholds
(Figure 2.6-4). The 25 fL threshold is repositioned at the valley to minimize interference to
the platelet parameter results. Therefore, the reported platelet results are acceptable. The MIC
(microcytes) flag appears to alert the operator that microcytes are present.
Figure 2.6-4 Microcytic Interference with a Valley between 18 fL and 25 fL
2 18 25fL 30
Microcytic Interference with a Valley below 18 fL

If the microcytes are extremely small so that the valley between the platelet population and
the microcyte population falls below the 18 fL limit, the threshold is placed at the 18 fL limit
(Figure 2.6-5). The MIC flag appears and the platelet count is flagged to alert the operator that
the extremely small microcytes present in this sample could not be eliminated. The platelet
count and associated parameters are not reliable and should be verified by an alternative
method. To effectively eliminate the microcytes, the Operator’s Guide suggests the customer
use platelet rich plasma (PRP) or a manual count to verify the results.
Figure 2.6-5 Microcytic Interference with a Valley below 18 fL
3 18 25fL 30
2.6-4 PN 4237616B
Interference with No Distinct Valley
Interference present in the upper area of the platelet distribution curve that blends with the
platelet population so that there is no clear distinction between the platelets and the
interference suggest the presence of schistocytes (fragmented red cells) or platelet aggregates
(platelet clumps).
If the threshold cannot be positioned in the 25 fL to 18 fL region, the threshold defaults to the
18 fL position (Figure 2.6-6). The SCH (schistocytes) flag appears and the platelet count is
flagged to alert the operator that the interference (which is most likely either schistocytes or
platelet clumps) could not be eliminated. The platelet count and associated parameters are
not reliable and must be verified using an alternative method.
Figure 2.6-6 Interference with no Distinct Valley
2 18 25fL 30
PN 4237616B 2.6-5
2.6-6 PN 4237616B
WBC PARAMETER DEVELOPMENT 2
2.7 WBC PARAMETER DEVELOPMENT
Overview
WBC parameter results are generated from two different dilutions: the 1:200 WBC/BASO
dilution which is made and analyzed in the WBC/BASO bath and the 1:80 DIFF dilution
which is made in the DIFF bath but analyzed in the flow cell.
WBC/BASO Dilution
The WBC and basophil counts are determined from the 1:200 dilution made in the
WBC/BASO bath. To make this dilution, 10 µL of whole blood is mixed with 2,000 µL of
AC•T 5diff WBC Lyse reagent. The reaction that occurs lyses the red blood cells and
specifically differentiates between basophils and other leukocytes by volume.
WBC Count
obtain an WBC count result, the instrument compares the data from the two 5-second count
periods then votes and rejects any questionable data. This is the reference WBC count, which
is also the count reported.
A second WBC count is determined in the flow cell during acquisition of the DiffPlot. The
two counts are compared and if they differ more than the predefined limit, a flag occurs.
WBC count: Number of cells per volume x calibration factor.
The WBC count is displayed and printed as: WBC = N x 103 cells /µL.
BASO Count
Differentiation between basophils and other leukocytes is obtained by means of the
AC•T 5diff WBC Lyse-specific lytic action. See Figure 2.7-1.
Figure 2.7-1 Areas Used to Determine WBC and BASO Parameter Results
b c d
WBC basophils
7615003A
In Figure 2.7-1, basophils are located in the area between the thresholds labeled c and d.
One hundred percent (100%) of the leukocytes is represented by the total number of
nucleated particles plus the basophils within the area between the thresholds labeled
b and d.
PN 4237616B 2.7-1
WBC PARAMETER DEVELOPMENT
The basophil percentage is calculated from the number of particles existing in the area
between the thresholds labeled c and d (Figure 2.7-1)
BASO count = Number of cells per volume x calibration factor in a percentage relative to the
number of counted cells (basophils plus other WBC nuclei).
BASO%
BASO count = ---------------------- × WBC count
WBC%
DIFF Dilution
The data for the DiffPlot is accumulated as the dilution made in the DIFF bath is injected into
the flow cell. To make the 1:80 DIFF dilution, 25 µL of the whole-blood sample is mixed with
1,000 µL of AC•T 5diff Fix reagent. The Fix reagent lyses the red blood cells, stabilizes the
the white blood cells, and differentially stains the lymphocytes, monocytes, neutrophils, and
eosinophils, with the eosinophils staining most intensely. After 12 seconds of incubation,
1,000 µL of AC•T 5diff Diluent reagent is added to stop the cytochemical reaction. This
dilution is injected through the flow cell 15 seconds. For 12 of these 15 seconds, data for
developing the DiffPlot is accumulated.
DiffPlot Development
The DiffPlot analysis on the AC•T 5diff hematology analyzer is based on three essential
principles:
r Dual Focused Flow (DFF) fluid dynamics, which is a process by which individual cells
or particles are focused in a stream of diluent (hydrodynamic focusing).
r The volume measurement (Coulter Principle).
r The measurement of transmitted light with zero degree (0°) angle, which permits a
response proportional to the internal structure of each cell and its absorbance.
From these measurements, a DiffPlot is developed with optical transmission (absorbance) on
the X-axis and volume on the Y-axis. Figure 2.7-2 shows the DiffPlot regions.
2.7-2 PN 4237616B
DiffPlot Regions Defined
The study of the DiffPlot permits the clear differentiation of four out of five leukocyte
populations. In a typical whole-blood sample, the basophil population is very small when
compared with the other four white cell populations.
Neutrophil (Neut)
Neutrophils, with their cytoplasmic granules and segmented nuclei, scatter light according to
their morphological complexity. A hypersegmented neutrophil gives an increased optical
response when compared to a young neutrophil population. The higher the complexity of the
cell, the further to the right they appear in the DiffPlot (Figure 2.7-2).
Lymphocyte (Lymph)
Lymphocytes, typically being small with regular shape are smaller in volume and lower in
absorbance than the other cells, and are positioned in the lower region of the DiffPlot
(Figure 2.7-2). Normal lymphocyte populations typically have a homogeneous volume with a
Gaussian (bell-shaped) distribution.
Large lymphocytes, reactive lymphoid forms, stimulated lymphocytes and plasma cells are
found in the upper portion of the lymphocyte region (Figure 2.7-2).
The lower area of the lymphocyte zone is normally empty; however, when small lymphocytes
are present, a population may exist in this area (Figure 2.7-2).
The presence of platelet aggregates is indicated by a distribution pattern that moves from the
DiffPlot origin into the lymphocyte region (Figure 2.7-2).
NRBC cytoplasmic membranes lyse like those of mature erythrocytes. The small nuclei that
remain appear in the debris and small lymphocyte regions (Figure 2.7-2).
Monocyte (Mono)
Monocytes are typically large cells with a kidney-shaped nucleus and agranular (granule-free)
cytoplasm. These cells neither scatter nor absorb large amounts of light and, therefore, are
positioned in the lower end of the absorbance axis. Due to their size, the monocytes are
clearly positioned high on the volume axis (Figure 2.7-2).
Very large monocytes may be found in the IMM (immature cell) region.
Eosinophil (Eos)
With the reagent action, eosinophils are the most intensely stained cells for optical
separation. Due to the staining and their size, the eosinophils will show higher absorbance
than the neutrophils, but will be of similar volume (Figure 2.7-2).
Debris
Platelets and debris from erythrocyte lysis represent the background debris population
located in the lower region of the DiffPlot.
PN 4237616B 2.7-3
Immature White Blood Cells
Immature Granulocytes
Immature granulocytes are detected by their larger volume and by the presence of granules
that increase the intensity of the scattered light.
Due to their increased volume and similar absorbance, promyelocytes, myelocytes, and
metamyelocytes are located above the neutrophil population and are typically counted as
IMM cells. IMM cells are included in the reported neutrophil value.
Band Cells
Band cells are typically larger or of similar size to the neutrophils; however, due to their low
level of cellular complexity, they absorb less light. As a result, band cells tend to appear in the
region between the neutrophils and the monocytes.
Blast Cells
Blast cells are generally larger than monocytes and have similar absorbance. When blast cells
are present, they are generally located above the monocytes, which means they will be
included in the IMM cell count.
Small blasts will be located between the normal lymphocyte and monocyte populations.
DiffPlot Thresholds
Most of the population partition thresholds are fixed and give the limits of the morphological
normality of leukocytes. Changes in the morphology of a population are expressed on the
DiffPlot by a shifting of the corresponding population. Volume and absorbance thresholds are
used to detect shifting populations. Volume thresholds and definitions are shown in
Figure 2.7-3. Absorbance thresholds and definitions are shown in Figure 2.7-4. The NL, NE
and MN alarms are also included in Figure 2.7-4.
2.7-4 PN 4237616B
Figure 2.7-3 Volume Thresholds
DL Threshold - Separates debris and small

lymphocytes.
DN Threshold - Separates debris and lower
neutrophils.
SL Threshold - Separates small lymphocytes and
lymphocytes.
LN Threshold - Separates neutrophils and lower
neutrophils.
DE Threshold - Separates debris and eosinophils.
LMN Threshold - Intersection point between the
lymphocyte, monocyte, and neutrophil
thresholds.
AL Threshold - Separates lymphocytes and
atypical lymphocytes.
LMU Threshold - Lower point on the separation
slope between atypical lymphocytes and
monocytes.
LMD Threshold - Upper point on the separation
slope between atypical lymphocytes and
monocytes.
MN Threshold - Upper point on the separation
slope between monocytes and neutrophils.
UM Threshold - Separates monocytes and upper
monocytes.
UN Threshold - Separates neutrophils and upper
neutrophils.
PN 4237616B 2.7-5
Figure 2.7-4 Absorbance Thresholds / NL, NE and MN Alarms
NL Threshold - Separates lymphocytes and

neutrophils.
RMN Threshold - Separates upper monocytes and
upper neutrophils.
NE Threshold - Separates neutrophils and
eosinophils.
NL, NE and MN Alarms
FNL - # of channels for NL alarm area.

FNE - # of channels for NE alarm area.
FMN - # of channels for MN alarm area.
2.7-6 PN 4237616B
PNEUMATIC/HYDRAULIC SYSTEM 2
2.8 PNEUMATIC/HYDRAULIC SYSTEM
Functions of Valves
Valve blocks are located close to the elements concerned. Five different blocks:
r Valves 1 to 11 (Figure 2.8-1):

t In the left side compartment behind the Main card,
t Horizontal block,
t Above the 5diff syringe and reagent syringes assembly.
t In the left side compartment behind the Main card,
t Vertical block,
t Beside the count syringe.
Figure 2.8-1 Valve 1 through Valve 16 Locations

t In the right side compartment,
t Horizontal block,
t At the top of the vertical traverse (above the sample syringe assembly).
Figure 2.8-2 Valve 17 and 18 Location
PN 4237616B 2.8-1
PNEUMATIC/HYDRAULIC SYSTEM
r Valve 19 is not used on the AC•T 5diff hematology analyzer.

t In the right side compartment,
t Vertical block,
t Beside the waste syringe.
t In the right side compartment (bath enclosure area),
t Horizontal block,
t Below the baths assembly.
Figure 2.8-3 Valve 20 to Valve 31 Locations
See Table 2.8-1 for a description of the functions for each valve in the AC•T 5diff hematology
analyzer.
2.8-2 PN 4237616B
Table 2.8-1 Valves and their Functions
Valve Function Action

1 Differential diluent Select flow cell sheath 2 / DIFF bath
2 Differential diluent Select flow cell sheath 1 / sheath 2
3 Differential diluent Select input/output for flow cell Diluent syringe
4 Flow cell sample supply Opens pathway from the DIFF bath to the flow cell
5 Flow cell sample injector Opens waste path for sample injector syringe
6 Hgb Lyse syringe flow Selects input/output of Hgb Lyse syringe
7 Rinse syringe flow Selects input/output of Rinse syringe
8 Fix syringe flow Selects input/output of Fix syringe
9 Diluent syringe flow Selects input/output of Diluent syringe
10 Diluent output control Routes diluent to probe rinse block or heating coil
11 WBC Lyse syringe flow Selects input/output of WBC Lyse syringe
12 Rinse output control Selects rinse to probe rinse block or WBC/BASO bath
13 Count syringe vent Opens vent line of count syringe
14 RBC/PLT count valve Opens vacuum count line for RBC bath
15 Diluent reservoir vent Selects between vacuum and vent for diluent reservoir
16 Count syringe drain Opens count syringe drain path
17 Probe rinse drain Opens drain line for probe rinse block
18 Probe diluent Routes diluent/rinse to sample syringe or rinse block
19 Spare Not used
20 Waste syringe vent Opens waste vent (through the rinse chamber)
21 Sweep flow diluent Routes diluent to heating coil or sweep flow
22 Diluent bath select Route diluent (via heating coil) to Hgb or RBC bath
23 WBC/BASO count vacuum Routes vacuum direct or through RBC/PLT count head
24 Flow cell drain Opens path from flow cell output to DIFF bath for drain
25 Diluent reservoir input Opens diluent source to diluent reservoir
26 Waste syringe control Selects waste to syringe / syringe waste out
27 Rinse chamber drain Opens drain path from rinse chamber
28 HGB bath drain Opens drain path from Hgb bath
29 DIFF bath drain Opens drain path from the DIFF bath
30 RBC bath drain Opens drain path from the RBC bath
31 WBC/BASO bath drain Opens drain path from WBC/BASO bath drain
PN 4237616B 2.8-3
Pneumatic Diagrams
To locate the pneumatic diagram for a desired reagent or the waste circuit, see the designated
figure:
r For the Hgb Lyse reagent circuit, see Figure 2.8-4.

r For the Fix reagent circuit, see Figure 2.8-5.
r For the WBC Lyse reagent circuit, see Figure 2.8-6.
r For the Rinse reagent supply circuit, see Figure 2.8-7.
r For the Probe Rinse reagent circuit, see Figure 2.8-8.
r For the WBC/BASO Rinse reagent circuit, see Figure 2.8-9.
r For the Diluent reagent circuit, see Figure 2.8-10.
r For the Probe Diluent reagent circuit, see Figure 2.8-11.
r For the Bath Diluent reagent circuit, see Figure 2.8-12.
r For the waste circuit, see Figure 2.8-13.
2.8-4 PN 4237616B
Figure 2.8-4 Hgb Lyse Reagent Circuit
PN 4237616B 2.8-5
Figure 2.8-5 Fix Reagent Circuit
2.8-6 PN 4237616B
Figure 2.8-6 WBC Lyse Reagent Circuit
PN 4237616B 2.8-7
Figure 2.8-7 Rinse Reagent Supply Circuit
2.8-8 PN 4237616B
Figure 2.8-8 Probe Rinse Reagent Circuit
PN 4237616B 2.8-9
Figure 2.8-9 WBC/BASO Rinse Reagent Circuit
2.8-10 PN 4237616B
Diluter System
Diluent Input (Figure 2.8-10)

Diluent for the AC•T 5diff hemtology analyzer enters a fitting at the rear of the instrument
from a diluent container and is stored in the diluent reservoir. The input tubing from the
diluent container should be no longer than 2 meters (78.7 in.) and the top of the container
cannot be lower than 80 cm (31.5 in.) from the input fitting on the instrument. Vacuum,
produced by the count syringe, is used to draw diluent into the diluent reservoir from the
diluent container. This vacuum is applied to port 2 of the diluent reservoir. Solenoid valve 15,
Diluent Reservoir Vent, is activated to connect the diluent reservoir to the count syringe.
When in the normal inactive state, this valve vents the diluent reservoir to atmosphere. The
vent tubing from port 1 of valve 15 is notched to ensure it does not seal against any surface
and is routed to the left side drip tray. A float sensor located in the reservoir is used to
determine when the reservoir is full, or needs more diluent.
Figure 2.8-10 Diluent Reagent Circuit
PN 4237616B 2.8-11
Two ports distribute diluent from the diluent reservoir. Port 1 supplies diluent to be used by
the flow cell while port 3 supplies diluent for the baths and aspirate probe. Solenoid valve 3
normally connects port 1 of the diluent reservoir to the center syringe piston in the 5diff
syringe assembly, allowing the syringe to fill with diluent. For diluent output from the 5diff
syringe see 5diff Syringe and Flow Cell.
Solenoid valve 9 connects the diluent reagent syringe to port 3 of the diluent reservoir, and
when it is in a normal inactive state, this path is open, allowing the syringe to be filled. For
output from the main diluent syringe, see Diluent to Baths and Probe and Probe Rinse.
5diff Syringe and Flow Cell

Diluent for several flow cell requirements originates at the center syringe piston of the 5diff
syringe assembly. When solenoid valve 3 is active, valves 1 and 2 have control of the diluent
path. If valve 2 is energized, diluent is sent from the large center syringe piston to (and
through) the left injector piston. A short upward stroke is used to fill the small left injector
piston with clean diluent. Since the center syringe piston is much larger, even a short stroke
will produce excess diluent, which exits out the top of the left injector piston and through the
flow cell.
When valve 2 is in the normal state, valve 1 determines where diluent is routed. An energized
valve 1 allows diluent to be sent through the heater assembly to port 2 of the DIFF bath. This
is used for the second part of the DIFF dilution when 1 mL of diluent is added to the sample
12 seconds after the original dilution with Fix. This stops the staining action of the Fix.
Solenoid valve 1 in its normal inactive state creates a diluent path for the flow cell outer
sheath. During normal flow cell operation, the center syringe piston (solenoid valve 3
energized and solenoid valves 1 and 2 in a normal inactive state) sends diluent through an
electrical isolator and T-fitting to ports 2 and 4 of the flow cell. This creates an outer sheath or
fluid pipe around the stream of fluid exiting the flow cell aperture. This sheath is
approximately 140 µm in diameter.
During the upward stroke of normal flow cell operation, the 5diff syringe assembly creates the
inner sheath flow. Diluent exits from the top of the left injector piston and enters the flow cell
at port 5. It then forms the inner sheath, creating a fluid pipe around the injected sample. The
sample injector creates sample flow of 40 µm in diameter, while the sample and inner sheath
is forced through the aperture, which is 60 µm in diameter. On exiting the aperture, an outer
sheath is created and this double sheath around the sample is called Dual Focused Flow, or
DFF.
Sample is sent through the flow cell with the right injector piston (see Figure 2.8-13), but it
first must be positioned. The sample dilution, created in the DIFF bath, is drained with the
waste syringe. The vacuum path to the waste syringe, starting at the bath, is through fluid
sensor M1, an energized solenoid valve 4, through the right injector piston (note that the
piston is not being used at this time), through an energized solenoid valve 5, T-fitting T6,
waste fluid sensor M2, a fluidic/electrical isolator, and solenoid valve 26 in its normal inactive
state. Fluid is drained from the bath until sensor M1 detects air. The length and size of the
tubing ensures that the sample does not actually reach or enter the right injector piston when
sensor M1 detects air and stops flow. This is critical. When the injector piston pushes up,
sample in the tubing between T-fitting T2 and port 5 of the right injector piston is sent
through the flow cell.
2.8-12 PN 4237616B
Probe and Probe Rinse
When valves 9 and 10 are both energized (Figure 2.8-11), diluent output from the diluent
syringe is sent to the probe and probe rinse block by way of solenoid valve 18. An energized
solenoid 18 routes diluent through the sample syringe and out the probe for backwash and
sometimes dilution. Note that the sample syringe is not used at this time. Since the piston seal
is an O-ring at the base of the piston, rather than a seal at the top of the piston, the bottom
fitting and top fitting on the syringe assembly have an open fluid path. Diluent is sent
through an inactive sample syringe by the diluent syringe. The sample syringe is only used
when aspirating or dispensing sample.
Figure 2.8-11 Probe Diluent Reagent Circuit
With solenoid valve 18 in its normal inactive state, the diluent is sent to fitting 1 on the probe
rinse block. This fluid exits the rinse block from fitting 2 and passes through solenoid valve
17 to the waste system. Usually, this occurs while the probe is moving up through the rinse
block to clean blood off the exterior of the probe. However, this is not always the case. On
occasion, solenoid 17 is not opened, and the probe does not move. Diluent is forced to flow
down the outside of the probe. This is done once to clean the exterior of the probe without
moving it and another time to add a small amount of diluent to a dilution.
Diluent to Baths
Energizing solenoid valve 9 routes the diluent syringe output through solenoid valve 10
(Figure 2.8-12). In the normal state of valve 10, diluent is routed for use at the sample baths.
There are 3 uses of diluent at the baths, diluent for dilution in the Hgb bath, diluent for
dilution in the RBC bath, and rinse for the WBC/BASO counting head.
PN 4237616B 2.8-13
Solenoid valve 21 routes diluent for dilutions through valve 22 when in a normal inactive
state. Valve 22 selects the RBC bath (normal, inactive state) or the Hgb bath (energized state).
The fluid paths to the baths both go through a heater block. Each path has a 1 mL coil of fluid
in the heater block which allows 1 mL of reagent to be heated to 35°C prior to being delivered
to the bath.
Figure 2.8-12 Bath Diluent Reagent Circuit
When solenoid valve 21 is energized, fluid is sent to the WBC/BASO counting head to rinse
the pathway and counting head. This is necessary since fluid in this area is used to flush cells
from the rear of the RBC/Plt aperture, a technique called the Rinse Flow System (RFS).
During the RBC/Plt count, the WBC/BASO count head is connected to the RBC/Plt count
head through an inactive (normal state) valve 23. The vacuum applied to the RBC/Plt
aperture actually draws fluid (rinse solution, not diluent) from the WBC/BASO bath, through
the WBC/BASO aperture, and past the rear of the RBC/Plt aperture, sweeping away any RBC
cells from the rear of the aperture. When solenoid valve 23 is energized, the WBC/BASO
counting head is connected directly to the counting syringe, providing vacuum for the
WBC/BASO count.
2.8-14 PN 4237616B
Waste System
The waste and drain system (Figure 2.8-13) comprises many components. The waste syringe
itself is used to drain the baths, and to expel waste from the baths. The count syringe expels
any waste that it accumulates during count directly into the waste system through normally
closed solenoid valve 16. Waste from the probe rinse block is pushed out by the diluent
syringe as well as being evacuated with the waste syringe.
The bath drain system connects each bath, including the rinse chamber, through a normally
closed solenoid valve to the waste system with a series of T-fittings. There is a fluid isolator
chamber between the baths and the waste syringe and container. This electrically isolates the
baths (and flow cell and aspirate probe) from any interference that can be picked up by the
external waste system. Draining waste from any bath involves opening the associated
solenoid valve while the drain syringe is filling. Solenoid valve 26 is then energized, which
connects the waste syringe to the external waste system, and waste is expelled.
There is also a fluid sensor, M2, in the waste system, just before the isolator. It is used to
detect whether the waste system has fluid or air at the appropriate times. It is not used to stop
a drain action, like M1 does when detecting that sample has been drained from the DIFF
bath.
Figure 2.8-13 Waste Circuit
PN 4237616B 2.8-15
2.8-16 PN 4237616B
ELECTRONIC SYSTEM 2
2.9 ELECTRONIC SYSTEM
Plug/Jack Labels
In some circumstances, connectors may not have matching plug/jack (P/J) labels. For
example, cable connector J5 plugs into board connector J5 on the traverse interconnect card.
Optical Preamplifier Card

This card:
r Performs an absorbance measurement.
r Is a voltage amplifier.
r Contains a photodiode that measures absorbance of light through the cell. The Optical
Preamplifier card returns an intensity proportional to the light signal. (The light signal is
proportional to the cell size, complexity and staining.)
LCD and Keypad Card

This card includes the keypad and LCD screen is connected to the Main card using one flat
cable through connector J1. The LED card is connected to this card through connector J4.
LED Card
This card supports the LEDs that are used to quickly identify instrument status. These LEDs
are located on the front panel.
r A red glowing LED indicates the instrument is busy.
r A green glowing LED indicates the instrument is ready for operation.
Motor Interconnect Card

All the different motors and end-of-run sensors are connected to this card. The Motor
Interconnect card is connected to the Main card using one flat cable through connector J2.
Traverse Interconnect Card

All the Traverse module electrical functions are connected to this card. The Traverse
Interconnect card is connected to the Main card using one flat cable through connector J5.
PN 4237616B 2.9-1
ELECTRONIC SYSTEM
2.9-2 PN 4237616B
SOFTWARE STRUCTURE 2
2.10 SOFTWARE STRUCTURE
Overview
The operating system software provides instrument I/O, system error checks, data analysis,
and individual diluter subroutines or functions. The operating system software also provides
diluter functions that energize solenoids, drive motors, and check sensors in the diluter.
Menu Trees
Most I/O functions of the operating system software are controlled by the user. This
interaction between the user and the instrument is called the user interface. A pushbutton
keypad and an LCD screen provide the physical user interface, while menu items provide the
software user interface. These menu items have associated arabic numerals.
The Main Menu consists of five options:
1. RUN SAMPLES
2. CALIBRATION
3. REAGENTS
4. DIAGNOSTICS
5. SETUP
How to Select a Menu Item
An operator may select a menu item two different ways,
1. Use the or arrow keys to highlight the desired menu item then press (ENTER
pushbutton on the instrument keypad) to select the highlighted option.
or
2. Simply press the numeric pushbutton (on the instrument keypad) that correlates with
the desired option. This is the faster way to select a menu item.
For example, to select the 3. REAGENTS menu item from the Main Menu, an operator may:
r Use the down arrow to highlight 3. REAGENTS then press ENTER .

or
r Press the pushbutton labeled 3 on the instrument keypad.
With the exception of Main Menu item 1. RUN SAMPLES, selecting a menu option produces a
submenu with a listing of additional options that may be selected.
See Figure 2.10-1 and Figure 2.10-2 for a graphic representation of the menus and menu
items (or options) available for the user and service personnel.
PN 4237616B 2.10-1
SOFTWARE STRUCTURE
Figure 2.10-1 User Menu Tree

MAIN MENU
CALIBRATION
1 - RUN SAMPLES
2 - CALIBRATION 1 - AUTOCALIBRATION
3 - REAGENTS 2 - CAL FACTORS
4 - DIAGNOSTICS 3 - PRINT CAL FACTORS
5 - SETUP 4 - REPRODUCIBILITY
PRIME
REAGENTS
1- DILUENT
1 - LEVEL-CHANGE 2 - FIX
2 - DAILY WORKLOAD 3 - WBC LYSE
3 - PRIME 4 - HGB LYSE RINSE
5 - RINSE
6 - ALL REAGENTS
7 - UNPRIME ALL 1 - BATHS
2 - FLOWCELL
DRAIN BATHS
DIAGNOSTICS DILUTER SYSTEMS
1 - RINSE
1-SYSTEM RESET CYCLE 1-BACKFLUSH 2 - FIRST DILUTION
2-MINI-PRIME 2-RINSE 3 - DIFF
3-DILUTER SYSTEM 3-DRAIN BATHS 4 - WBC / BASO
4-HARDWARE SYSTEM 4-EXTENDED CLEANING 5 - RBC / PLT
5-SERVICE 6 - ALL BATHS
7 - DILLUENT RESERVOIR
HARDWARE SYSTEMS
MOTORS
1-HARDWARE RESET
2-MOTORS * 1 - SAMPLING PROBE
3-VALVES * 2 - TRAVERSE
4-TRAVERSE SERVICE POSITION 3 - SAMPLING SYRINGE
4 - DRAINING SYRINGE
5 - COUNTING SYRINGE
6 - FLOWCELL SYRINGES
7 - DILUTION SYRINGES
VALVES
SERVICE
PASSWORD 1 - 1 TO 11
REQUIRED 2 - 12 TO 16
3 - 17 AND 18
4 - 20 TO 26
5 - 27 TO 31
SET DATE / TIME
SETUP 1 - TIME FORMAT
2 - DATE FORMAT
1 - DATE / TIME 3 - SET DATE & TIME
2 - UNITS
3 - LAB. LIMITS PATIENT RANGES
4 - HOST SETUP LAB. LIMITS
5 - PRINTER 1 - CBC
6 - OTHERS 1 - PATIENT RANGES 2 - DIFF
2 - ACTION RANGES
3 - FLAGS SENSITIVITY
4 - THRESHOLDS ACTION RANGES
HOST SETUP 1 - CBC

2 - DIFF
1 - HOST SETUP CONFIGURATION
2 - SENDING CONFIGURATION
3 - SENDING OPTIONS
4 - VARIABLE FORMAT SETUP VARIABLE FORMAT SETUP
5 - SEND LATEST RESULT
1 - NUMERICAL RESULTS
2 - FLAGS AND MESSAGES
PRINTER 3 - HISTOGRAMS AND THRESHOLDS
4 - PATIENT FILE
1 - PRINTER CONFIGURATION
* Consult a Beckman Coulter 2 - INSTITUTIONNAL HEADER
representative before selecting this 3 - PRINT LATEST RESULT
option.
Service password required CALIBRATION
OTHERS
1 - CV% L I M I TS
1 - CALIBRATION 2 - DEFINE OPERATOR
2 - IDENTIFICATION MODE
3 - AUTOCLEAN FREQUENCY LANGUAGE
4 - CHANGE PASSWORD
5 - LANGUAGE 1 - ENGLISH
6 - REAGENT VOLUMES 2 - FRENCH
7616071B 7 - CYCLE COUNTS 3 - GERMAN
8 - PRINT SYSTEM SETUP 4 - SPANISH
9 - SEND SETUP 5 - ITALIAN
2.10-2 PN 4237616B
SOFTWARE STRUCTURE 2
Figure 2.10-2 Service Menu Tree
MAIN MENU
1 - RUN SAMPLES
2 - CALIBRATION
3 - REAGENTS
4 - DIAGNOSTICS
5 - SETUP
DIAGNOSTICS
1-SYSTEM RESET CYCLE
2-MINI-PRIME
3-DILUTER SYSTEM
4-HARDWARE SYSTEM
5-SERVICE
SERVICE
PASSWORD
REQUIRED
MEASURMENT
SERVICE
1 - HGB BLANK ADJUSTMENT
1 - DILUTION 2 - APERTURE CURRENT
2 - MEASUREMENT 3 - RBC / PLT GAIN
3 - HEATING SYSTEMS 4 - WBC / BASO GAIN
4 - MIXING 5 - DIFF ADJUSTMENT
5 - SENSOR CHECK 6 - PULSE ADJUSTMENT
6 - VACUUM CHECK
7 - BURN-IN
8 - FLOWCELL WBC CALIBRATION HEATING SYSTEMS
9 - OTHERS
1 - HEATING COIL
2 - BATH ENCLOSURE
SENSOR CHECK
1 - DRAINING
2 - DIFF TRANSFER HEATING COIL
1 - ADJUSTMENT
2 - REFERENCE
VACUUM CHECK
1 - COUNTING BATH ENCLOSURE
2 - DRAINING
1 - ADJUSTMENT
2 - REFERENCE
BURN - IN
1 - BURN - IN CYCLES
2 - ANALYSIS CYCLES
FLOWCELL CALIBRATION
1 - AUTOCALIBRATION
2 - CAL FACTORS
OTHERS
7616070B 1 - USER MODE
2 - CYCLE COUNTS
3 - PARK SYRINGES
4 - RESET TO DEFAULT VALUES
PN 4237616B 2.10-3
SOFTWARE STRUCTURE
2.10-4 PN 4237616B
CONTENTS
3 INSTALLATION PROCEDURES, 3.1-1
3.1 PREINSTALLATION CHECKS, 3.1-1

Environment, 3.1-1
Altitude Range, 3.1-1
Ambient Temperature, 3.1-1
Space and Accessibility Requirements, 3.1-1
Electrical Input, 3.1-1
Power Requirements, 3.1-2
Grounding, 3.1-2
Installation Category, 3.1-2
Electromagnetic Environment Check, 3.1-2
Inspection Report, 3.1-2
3.2 INITIAL SETUP, 3.2-1

Preinstallation Checks, 3.2-1
Supplies, 3.2-1
Unpacking, 3.2-1
Inspection, 3.2-1
Unpack the Analyzer, 3.2-1
Unpack the Installation Kit, PN XEA484A, 3.2-1
Unpack the Waste Alarm Kit, PN 6912680, 3.2-1
Verify All Caution and Compliance Labels are in Place, 3.2-1
Connect the Waste System, 3.2-3
Connect the Waste Tubing, 3.2-3
Install the Waste Alarm, 3.2-3
Connect the Reagents, 3.2-5
Connect the Diluent Tubing, 3.2-5
Install the Reagent Bottles, 3.2-6
Install the Printer, 3.2-7
Power On the Instrument, 3.2-7
Enter Reagent Lot Numbers, 3.2-8
Prime the Instrument, 3.2-8
Configure the Instrument Printer Settings, 3.2-9
Set the User Mode, 3.2-9
3.3 PRINTER INSTALLATION, 3.3-1

EPSON® LX™- 300 and LX™- 300+ Printer Connection, 3.3-1
Unpack the Printer, 3.3-1
Install the Knob, 3.3-1
Install the Ribbon Cartridge, 3.3-2
Connect the Printer, 3.3-3
Paper Feed Options, 3.3-4
Single Sheet Paper Feed Setup, 3.3-4
Loading Continuous Feed Paper Feed, 3.3-5
Configure the Printer, 3.3-7
LX300 Printer, 3.3-7
LX300+ Printer, 3.3-7
Complete the Instrument Installation, 3.3-9
PN 4237616B 3-i
CONTENTS
ILLUSTRATIONS
3.3-1 Carefully Remove All Packing Materials, 3.3-1
3.3-2 Paper-Feed Knob Installation, 3.3-1
3.3-3 Insert the Ribbon Cartridge, 3.3-2
3.3-4 Threading the Ribbon, 3.3-2
3.3-5 Proper Ribbon Placement, 3.3-3
3.3-6 Cable Connection at the Instrument, 3.3-3
3.3-7 Cable Connections, 3.3-4
3.3-8 Paper Support for Printing Single Sheets of Paper, 3.3-5
3.3-9 Printer Sprockets, 3.3-5
3.3-10 Replace Paper Guide, 3.3-6
3.3-11 Printer Ready for Continuous Feed Printing, 3.3-6
3.3-12 LX300 Printer Control Panel, 3.3-7
3.3-13 LX300+ Printer Control Panel, 3.3-7
TABLES
3.1-1 Space Requirements, 3.1-1
3.2-2 Calibration Factors - Acceptable Range, 3.2-11
3.3-1 LX300+ Printer Controls and Indicators, 3.3-7
3.3-2 LX300+ Printer Default Settings, 3.3-8
3-ii PN 4237616B
3INSTALLATION PROCEDURES 3
3.1 PREINSTALLATION CHECKS
Prior to installing the AC•T 5diff hematology analyzer, a pre-site inspection is required to
verify the following conditions:
Environment
The AC•T 5diff analyzer should be operated in an indoor location only.
Altitude Range
The AC•T 5diff hematology analyzer may be operated at any altitude up to 3,000 meters
(9,843 feet). Operation at an altitude over 3,000 meters (9,843 feet) is not recommended.
Ambient Temperature
The ambient operating temperature is 16 to 34°C (61 to 93°F). If the AC•T 5diff analyzer is
kept at a temperature less than 10°C (50°F), the instrument should be allowed to set at a
proper room temperature for one hour before use.
Space and Accessibility Requirements

The AC•T 5diff hematology analyzer should be placed on a clean and level table or work
station. Please note that the instrument, printer, and reagents weigh approximately 37 kg
(81 lbs).
WARNING Risk of operator injury if only one person lifts the instrument. The instrument weighs more than
18 Kg (40 lbs.) and has no lifting handles. To prevent injury, at least two people following necessary safety
precautions should lift the instrument together.
The diluent container may be placed on the same level as the instrument or below. If placed
on the floor, the top of the diluent container cannot be lower than 80 cm (31.5 inches) from
the table level of the instrument. The diluent and waste tubings are limited in length to a
maximum of 2 meters (78 inches).
Check the site for proper space allocation (Table 3.1-1).
Table 3.1-1 Space Requirements
Linear Dimensions Required by Instrument

Height 58.0 cm (23.0 inches)
Width 44.4 cm (17.5 inches)
Depth 50.1 cm (19.8 inches) plus an additional 20.0 cm (8.0 inches) for proper ventilation
Electrical Input
IMPORTANT Risk of erroneous results. If an extension cord is used, electrical interference could affect the
instrument’s operation and results. Locate the instrument close enough to a power outlet that an extension
cord is not necessary.
Check for the availability of a power connector. Make sure the instrument is close enough to
a power outlet that the ac power cable safely reaches it. The ac power cable is 1.8 meters
(6 feet) long and is attached to the back of the instrument, in the lower right corner.
PN 4237616B 3.1-1
INSTALLATION PROCEDURES
PREINSTALLATION CHECKS
Power Requirements
Verify the wall socket is an outlet capable of supplying 100 Vac to 240 Vac, from 50 Hz to
60 Hz.
Grounding
Proper grounding is required. Verify the ground (earth) for the wall plug is correctly
connected to the laboratory grounding electricity installation. If there is no ground, use a
ground stake. Current electricity standards must be applied.
Installation Category
This instrument is designed to be safe for transient voltages according to Installation
Category II and Pollution Degree 2.
Electromagnetic Environment Check

The AC•T 5diff analyzer produces less than the acceptable level of electromagnetic
interference when properly placed. Electromagnetic interferences are limited to levels that
allow the correct operation of other instruments conforming to their placement.
To avoid problems, make sure the instrument is not placed near electromagnetic fields or
shortwave emissions (such as radar, X-ray machines, scanners, and so forth).
Inspection Report
Review the findings with your contact person. If deficiencies are present, make sure the
customer understands what actions are necessary to meet the specifications for the system.
Establish a time frame for completion. Notify your manager if the installation must be
rescheduled.
3.1-2 PN 4237616B
INITIAL SETUP 3
3.2 INITIAL SETUP
Preinstallation Checks
If any deficiencies were noted during the preinstallation check, verify they are resolved before
installing the instrument.
Supplies
Make sure an adequate supply of reagents, controls, and calibrator are available at the site.
For details, see Chapter 1 of the Operator’s Guide.
Unpacking
Inspection
Inspect all boxes for damage. Notify shipping of external damage.
Unpack the Analyzer

1. Unpack the analyzer and place it on the table or bench as determined in the
preinstallation site inspection.
2. Check the instrument for damage.
Unpack the Installation Kit, PN XEA484A

1. Unpack the Installation kit.
2. Using the parts list in Table 8.1-8, ensure that no parts are missing.
Unpack the Waste Alarm Kit, PN 6912680

1. Unpack the Waste Alarm kit.
2. Using the packing slip, ensure that no parts are missing.
3. Make sure the additional parts needed to complete the installation are available:
r Two sets of Velcro strips, precut (2" x 1")
t Hook strip, PN 1017414 (need 2)
t Loop strip, PN 1017413 (need 2)
r Tubing, 035 clear polyurethane, 2-inch piece, PN 3202035
r Feed-through fitting, PN 6216308
Verify All Caution and Compliance Labels are in Place

1. Verify the caution label on the back of the instrument is in place (Figure 3.2-1).
PN 4237616B 3.2-1
INITIAL SETUP
Figure 3.2-1 Warning and Caution Label Locations on the Instrument
M O D c
M O D
N O .
A S S Y
N O . x x x x x x S /N
x x x x x x
N O . A •T 5 d i f f
1 0 0 -2 4 0 5 0 /6 0
MANUFACTURED BY COULTER CORPOR ATION
BECKMAN 11800 A BECKM AN COULTER COMPANY
COULTER SW 147 AVEN UE, MIAMI, FLOR IDA 33196-2500 U.S.A.
PATTEN TS ISSUED AND/OR PEN DING
A S S Y
x x x x x x x x x x x x
C A U T IO N :
S /N
N O .
O R B A C K .
R E F E R S E R V I C IN G T O Q U A L I F I E D S E R V IC E P E R S O N N E L .
E L E C T R IC S H O C K H A Z A R D . D I S C O N N E C T U N IT F R O M P O W E R S O U R C E
P R IO R T O S E R V I C I N G .
F O R C O N T I N U E D P R O T E C T I O N A G A I N S T R F IR E H A Z A R D , R E P L A C E O N L Y
W I T H S A M E T Y P E A N D R A T IN G O F F U S E .
1 0 0 -2 4 0 5 0 -6 0 0 .9 -2 .0 2 0 0
MANUFACTURED FOR BECKMAN COULTER INC.
BECKMAN 11800 SW 147 AVENUE, MIAMI, FLORIDA 33196-2500 U.S.A.
PATENTS ISSUED AND/OR PENDING
COULTER MADE IN FRANCE
C A U T IO N :
O R B A C K .
F O R S A F E T Y R E A S O N S , E Q U IP M E N T R E Q U IR E S C O N N E C T IO N T O
T H IS A R E A M A Y C O N T A IN
B IO H A Z A R D O U S M A T E R IA L
R E F E R T O P R O D U IC T R E F E R E N C E
M A N U A L F O R P R O P E R H A N D L IN G
C A U T IO N
A L L C O V E R S /P A N E L S M U S T B E
S E C U R E D IN P L A C E P R IO R T O
IN S T R U M E N T O P E R A T IO N .
R E F E R T O P R O D U C T R E F E R E N C E
M A N U A L F O R P R O P E R IN S T A L L A T IO N . 7615046B
2 4 2 9 5 5 5
2. Open the right side door. This door is fastened with two partial-turn, captive-slotted
screws.
3. Remove the two plastic blockers from the traverse assembly, one from the horizontal and
a second from the vertical traverse guide rod. See Figure 3.2-2.
Figure 3.2-2 Plastic Blocker Locations, View with Right Door Open
3.2-2 PN 4237616B
INITIAL SETUP 3
4. Manually push the sample probe assembly towards the front of the instrument and verify
the warning label is in place (Figure 3.2-1).
5. Remove the four hex screws securing the left side panel to the instrument frame and
verify the caution label is in place (Figure 3.2-1).
6. Replace the left side panel.
Connect the Waste System
Connect the Waste Tubing

ATTENTION: The waste tubing is limited in length to a maximum of 2 meters (78 inches).
1. Attach one end of waste tubing (4x6 mm / PN - EAE028A) to Luer fitting

(PN - EAC019A).
2. Connect the Luer fitting to the lower waste fitting on the back of the instrument
(Figure 3.2-3).
Figure 3.2-3 Rear Panel Connections
M O D
N O .
A S S Y
N O . x x x x x x S /N x x x x x x
1 0 0 -2 4 0 5 0 /6 0
MANUFACTURED BY COULTER CORPORATION
BECKMAN A BECKMAN COULTER COMPANY
11800 SW 147 AVENUE, MIAMI, FLORIDA 33196-2500 U.S.A.
COULTER PATTENTS ISSUED AND/OR PENDING
C A U T IO N :
O R B A C K .
Luer fitting
B A R C O D E
P R IN T E R
D IL U E N T
W A S T E
Waste fitting
Waste tubing
(< 78 in.)
Install the Waste Alarm

If a 20 L container is used for waste, connect the waste alarm as follows:
1. Remove the battery cover and install the battery in the alarm casing. Replace the cover.
2. One at a time, remove the adhesive backing from a loop velcro strip and attach it to the
back of alarm casing. The placement must be opposite the battery cover, in the left and
right corners (Figure 3.2-4).
PN 4237616B 3.2-3
INITIAL SETUP
Figure 3.2-4 Loop-Side Velcro Strip Attachment
Battery Velco strip-

cover hook side
Velco strip-
loop side
7615054A
3. Without removing the adhesive backing, attach the hook fastener of the velcro strips to
the loop strips.
4. Plug the float sensor connector P101 into the alarm connector J101.
5. Invert the float to activate a loud, repetitive beep.
r If the alarm is working properly, separate the connectors.
r If a low chirp sound occurs instead, the battery is low. Replace the battery.
6. Install the 2-inch piece of 035 tubing (Figure 3.2-5) on the large feed-through fitting
(threaded side). Firmly seat.
Figure 3.2-5 Waste Alarm and Float Sensor Setup
J101
P101
Feed-through
fitting
Waste
2-inch piece
alarm
035 tubing
Float
sensor
20 L Waste
container
7616081A
7. Install the other end of tubing on the top of float sensor port. Firmly seat.
8. Insert the other end of fitting (unthreaded side) into waste tubing.
Note: Make sure that tubing is installed over both barbs.
9. Place the float sensor in a 20-liter waste container. Screw the cap on tightly.
3.2-4 PN 4237616B
INITIAL SETUP 3
10. Install the waste alarm on the back of the instrument as follows:
a. While holding the alarm casing with its connector hanging freely downward,
remove the adhesive backing from the hook side of the velcro strips.
b. Attach the alarm to the rear access panel (Figure 3.2-6).
Figure 3.2-6 Position the Waste Alarm on the Rear Access Panel
M O D
N O .
A S S Y
1 0 0 -2 4 0 5 0 /6 0
C A U T IO N :
O R B A C K .
C O U L T E R
11. Plug the float sensor connector P101 into the alarm connector J101.
Connect the Reagents
IMPORTANT Risk of misleading results. If a reagent pickup tube is contaminated, bacterial and/or fungal
growth may occur inside the reagent container. This growth may cause unacceptable background results
especially for Plts. When connecting the reagent pickup tubes, the straw portion of the pickup tube should
not be touched or laid on an uncovered tabletop. Ensure the reagent pickup tubes remain clean and free of
contamination.
Connect the Diluent Tubing

ATTENTION: The diluent tubing is limited in length to a maximum of 2 meters (78 inches).
The diluent container may be placed on the same level as the instrument or below. If placed
on the floor, the top of the diluent container cannot be lower than 80 cm (31.5 inches) from
the table level of the instrument.
1. Attach one end of diluent tubing (3x6 mm / PN - EAE011A) to Luer fitting
(PN - EAC019A) and the other to pickup tube (PN - XEA018A).
2. Connect the Luer fitting end to the upper fitting on the rear panel of the instrument
(Figure 3.2-3, item 2).
3. Insert stopper FBL001A into the container and insert the diluent pickup tube through
the stopper.
PN 4237616B 3.2-5
INITIAL SETUP
Install the Reagent Bottles

1. Open the reagent compartment door (on the front of the instrument).
a. Note the color-coded label on the back panel. The color coding on the instrument
label correlates with the color coding on reagent labels (Figure 3.2-7).
b. Note the four tubings hanging inside the compartment. Once a bottle stopper is
assembled, the assembly is attached to the tubing hanging in its color-coded area
and inserted into the reagent bottle. The bottle is then positioned inside the reagent
compartment as shown in Figure 3.2-7.
Figure 3.2-7 Reagent Bottle Locations
Rinse Fix Hgb WBC Lyse

Lyse
Rinse Fix Hgb WBC Lyse

Lyse
2. Install the WBC Lyse reagent bottle (Figure 3.2-7):

a. Loosen the cap of a new WBC Lyse reagent container (yellow label).
b. Assemble pickup PN - GBG144A with cap PN - GAK302A.
c. Connect the stopper assembly to the tubing labeled WBC Lyse hanging inside the
reagent compartment.
d. Without lying the assembly down, remove the cap and ring from the new reagent
container and insert the stopper assembly into the WBC Lyse reagent bottle.
e. Place the reagent bottle inside the compartment. Position it in front of the yellow
WBC Lyse portion of the compartment label.
3.2-6 PN 4237616B
INITIAL SETUP 3
3. Install the Hgb Lyse reagent bottle (Figure 3.2-7):
a. Loosen the cap of a new Hgb Lyse reagent container (orange label).
b. Assemble pickup PN - GBG145A with cap PN - GBG155A.
c. Connect the stopper assembly to the tubing labeled Hgb Lyse hanging inside the
container and insert the stopper assembly into the Hgb Lyse reagent bottle.
e. Place the reagent bottle inside the compartment. Position it in front of the orange
Hgb Lyse portion of the compartment label.
4. Install the Fix reagent bottle (Figure 3.2-7):
a. Loosen the cap of a new Fix reagent container (green label).
b. Assemble pickup PN - GBG144A with cap PN - GAK302A.
c. Connect the stopper assembly to the tubing labeled Fix hanging inside the reagent
compartment.
container and insert the stopper assembly into the Fix reagent bottle.
e. Place the reagent bottle inside the compartment. Position it in front of the green Fix
portion of the compartment label.
5. Install the Rinse reagent bottle (Figure 3.2-7):
a. Loosen the cap of a new Rinse reagent container (blue label)
a. Assemble pickup PN - GBG144A with cap PN - GAK302A.
b. Connect the stopper assembly to the tubing labeled Rinse hanging inside the
c. Without lying the assembly down, remove the cap and ring from the new reagent
container and insert the stopper assembly into the Rinse reagent bottle.
d. Place the reagent bottle inside the compartment. Position it in front of the blue
Rinse portion of the compartment label.
Install the Printer

If you have not already done so, install the printer using the procedure under Heading 3.3,
PRINTER INSTALLATION. Once the printer is installed, return to this procedure to complete
the instrument installation and verification.
Power On the Instrument

1. Connect the power cord to the back of the instrument (Figure 3.2-3, item 7).
2. Plug the instrument into the ac power source.
3. Turn the instrument on.
Note: At the factory, a new instrument is left in the Manual Startup mode so the
automatic startup is bypassed when the instrument is powered on.
PN 4237616B 3.2-7
INITIAL SETUP
Enter Reagent Lot Numbers

ATTENTION: A reagent lot number contains 11 alpha/numeric characters consisting of five
numeric digits, an alphabet letter, and five more numeric digits. For example, the lot number
for Diluent might be 00102D00002.
r The digits in a lot number are entered using the numeric keypad on the front of the
instrument.
r The letter is added using the up arrow key. The first time the up arrow key is pressed, the
letter A appears on the screen, the next time the up arrow key is pressed, the letter B
appears, and so forth. In the example above, the up arrow key needs to be pressed four
times to display the letter D.
r Pressing the right arrow key saves the letter and moves the cursor to the next entry
position.
1. From the Main Menu, select 3. REAGENTS tt 1. LEVEL - CHANGE.
2. Press the down arrow key as many times as necessary to highlight the CHANGE ALL bar
then press ENTER. The lot number prompt appears for the reagent.
3. Enter the reagent lot number using the instrument keypad as follows:
a. Press the numeric keys that correspond with the first five digits.
b. Press the up arrow key as many times as necessary to display the required letter.
c. Press the right arrow key to save the letter and move to the next entry position.
d. Press the numeric keys that correspond with the last five digits.
e. Press ENTER to continue to the next reagent.
f. Repeat steps a through e for each reagent. When the last lot number is entered, the
instrument automatically initiates a prime reagent routine.
4. When the prime reagent routine is done,
a. Verify all the reagents levels are near 100%.
b. Press ESC to return to the Reagents menu.
Prime the Instrument

1. From the Reagents menu, select 3. PRIME tt 6. ALL REAGENTS to prime the reagents again.
Note: This second prime is necessary because this an installation. After installation, a
reagent line is thoroughly primed and the prime routine done at the end of the lot
number change is sufficient.
2. Visually inspect the reagent lines and pumps for air bubbles and repeat the priming if air
bubbles are still present.
3.2-8 PN 4237616B
INITIAL SETUP 3
Configure the Instrument Printer Settings
Configure the printer settings, as needed, including.
r Paper length (inches): 5.5 inches, 6 inches, 11 inches, or 12 inches. The paper in the
installation kit is 12 inches long.
r Area printing: Options 1 through 3.
r Patient range printout: Prints normal ranges.
r Messages printout: Prints interpretive messages.
r Print Raw Values: Prints raw data. Select this option only for troubleshooting purposes,
not for routine operation.
r Zoomed Print Screen: Allows large printout of screen display.
r Disable printer: Does not print the results and does not sound a printer alarm.
1. From the Main Menu, select SETUP tt PRINTER tt PRINTER CONFIGURATION (Figure 3.2-8).
Figure 3.2-8 Printer Configuration Menu
PRINTER CONFIGURATION 02/27/00 16:05

PAPER LENGTH (INCHES) AREA PRINTING
5.5" OPTION 1
6" OPTION 2
11" OPTION 3
12"
PATIENT RANGE PRINTOUT
MESSAGES PRINTOUT
DIFFPLOT & HISTOGRAM FLAGS
HISTOGRAM THRESHOLDS
PRINT RAW VALUES
ZOOMED PRINT SCREEN
DISABLE PRINTER
2. At the instrument keypad, use the arrow keys to move the blinking cursor into the box
next to the desired option.
Note: If you press the decimal point key too long, the dot may appear then disappear.
The dot inside the box indicates the option is selected.
a. If the box is empty, pressing the decimal point key places a dot inside the box. The
dot indicates the option is selected.
b. If the box has a dot, the option is already selected. Pressing the decimal point key
removes the dot which de-selects the option.
3. When all desired selections are made, press ESC to save and exit.
4. Press ESC as many times as necessary to return to the desired menu.
5. Printer configuration is detailed in Appendix A of the Operator’s Guide.
Set the User Mode

At the factory, a new instrument is left in the Manual Startup mode so the automatic startup is
bypassed when the instrument is powered on. To make sure the system is primed and
operational after the Customer has turned the power off then back on, the Manual Startup
mode should be disabled.
PN 4237616B 3.2-9
INITIAL SETUP
Disable the Manual Startup mode as follows:
1. From the Service menu, select 9. OTHERS tt 1. USER MODE. The blinking cursor is inside
the MANUAL STARTUP box. If you look closely, you should also be able to distinguish a
dot inside the box.
2. At the instrument keypad, press the decimal point key. Make sure the dot no longer
appears inside the MANUAL STARTUP box. The automatic startup is reactivated.
Note: If you press the decimal point too long, the dot may reappear. The dot inside the
MANUAL STARTUP box indicates the Manual Startup mode is selected.
3. Press ESC to save the selection.
IMPORTANT Risk of erroneous Hgb results. The right side door must be closed during Startup. Bypassing
the right side door interlock and running the Startup with the right side door open may generate a (. . . . )
code for the Hgb blank. Make sure the right side door is closed at least 5 minutes before running a Startup.
4. Turn the instrument OFF for about five seconds, then turn the instrument back ON
again. The power ON sequence should now perform a Startup and background cycle.
This sequence also establishes a Hgb blank reference which is used as a Hgb blank check
during normal sample analysis.
Verification
1. Verify the Startup results passed. If the results do not pass, press STARTUP on the
instrument keypad to repeat the Startup and background check.
2. Use a fresh normal whole-blood specimen to check instrument reproducibility.
a. From the Main Menu, select 2. CALIBRATION tt 4. REPRODUCIBILITY.
b. Run eleven samples and then delete the first one for a ten-shot reproducibility.
c. Verify the reproducibility results are within acceptable limits. See Table 3.2-1.
.
Table 3.2-1 Whole-Blood Reproducibility CV Limits for 20 Cycles
Parameter %CV Test Level

WBC < 2.0% at 10.0 x 103/µL
RBC < 2.0% at 5.00 x 106/µL
Hgb < 1.0% at 15.0 g/dL
Hct < 2.0% at 45.0%
MCV < 1.0% at 90.0 fL
Plt < 5.0% at 300 x 103/µL
3. Have the Customer perform the calibration procedure using the instructions in
Chapter 7 of the Operator’s Guide.
3.2-10 PN 4237616B
INITIAL SETUP 3
4. Verify the calibration factors are acceptable according to Table 3.2-2.
.
Table 3.2-2 Calibration Factors - Acceptable Range
Minimum Maximum
Parameter Acceptable Value Acceptable Value
WBC 90 200
RBC 160 290
Hgb 25.0 55.0
Hct 160 290
Plt 180 400
RDW 0.1 0.9
PN 4237616B 3.2-11
INITIAL SETUP
3.2-12 PN 4237616B
PRINTER INSTALLATION 3
3.3 PRINTER INSTALLATION
EPSON® LX™- 300 and LX™- 300+ Printer Connection
Unpack the Printer

1. Unpack the printer and set it on a flat, stable surface.
2. Carefully remove all packing materials including the tabs shown in Figure 3.3-1.
Figure 3.3-1 Carefully Remove All Packing Materials
Install the Knob

Install the paper-feed knob (Figure 3.3-2).
Figure 3.3-2 Paper-Feed Knob Installation
PN 4237616B 3.3-1
PRINTER INSTALLATION
Install the Ribbon Cartridge

1. Open the printer cover to the upright position, then pull it up to remove it.
CAUTION Risk of damage to the printer. Never move the print head while the printer is turned on. Do not
move the print head unless the power is turned OFF.
2. Slide the printer head to the middle of the printer.

3. Locate the ribbon cartridge and turn the ribbon-tightening know in the direction of the
arrow to remove any slack from the ribbon.
4. Insert the ribbon cartridge into the printer as shown in Figure 3.3-3. Press both sides of
the cartridge firmly to fit the plastic hooks into the printer slots.
Figure 3.3-3 Insert the Ribbon Cartridge
5. Guide the ribbon between the print head and ribbon guide (Figure 3.3-4).
Figure 3.3-4 Threading the Ribbon
3.3-2 PN 4237616B
6. Make sure the ribbon is positioned between the print head and the ribbon guide. You
may use a pointed object, such as a ball point pen to help guide it into place
(Figure 3.3-5). Once in place, turn the ribbon tightening knob to help position the
ribbon. It should not be twisted or creased.
Note: This ribbon placement can also verified later when the paper is installed. If the
ribbon is properly installed, the paper and ribbon do not make direct contact.
Figure 3.3-5 Proper Ribbon Placement
7. Manually slide the printer head from side to side to make sure it moves smoothly.
Connect the Printer
CAUTION Risk of damage to the instrument. Connecting the printer to the instrument with the power on
could damage the instrument. Ensure the instrument’s power is OFF before connecting the printer.
1. Make sure the instrument’s power is off.

2. Locate the printer cable delivered with the instrument.
3. Notice that one connector has two screws and the other connector has two clips.
4. At the back of the instrument, attach the connector with the two screws to the connector
located in the lower right corner of the instrument (Figure 3.3-6).
Figure 3.3-6 Cable Connection at the Instrument
PN 4237616B 3.3-3
5. Tighten the screws to secure the cable connection.

6. At the printer, lift the printer and set it on its side as shown in Figure 3.3-7.
Figure 3.3-7 Cable Connections
7. Attach the other end of the cable to the printer as shown in Figure 3.3-7. Lock the
connector in place with its two side clips.
8. If a 220 Vdc printer is being installed, insert the power cord in the printer (Figure 3.3-7).
Note: The power cord for the 120 Vdc printer is already attached.
9. If the laboratory will be using continuous feed printing, guide the interface cable and the
power cord through the cable slots on the left and right sides of the printer to keep the
cables from blocking the paper supply. Both cables can be routed to one side.
10. Set the printer flat on the table.
11. Plug the printer power cord into the ac power source.
Paper Feed Options

An operator may choose continuous feed printing or printing on single sheets of paper. Set up
the option your customer desires to use on a routine basis.
Single Sheet Paper Feed Setup

1. Install the paper supports as shown in Figure 3.3-8. Use this support only when printing
on single sheets of paper is desired.
2. Set the paper selection tab to single sheet Figure 3.3-8.
3.3-4 PN 4237616B
Figure 3.3-8 Paper Support for Printing Single Sheets of Paper
3. See the printer user manual for additional information.
Loading Continuous Feed Paper Feed

1. Place the paper selection tab to continuous feed Figure 3.3-8.
2. Make sure the printer is turned off.
3. Remove the paper guide that separates the incoming paper from the printed paper.
4. Release the sprocket units by pulling the sprocket locks forward (Figure 3.3-9).
Figure 3.3-9 Printer Sprockets
5. The printer prints to the right of the position marked 0. Slide the left sprocket unit to
position the paper then push the sprocket lock lever back to lock it in place. Slide the
right sprocket unit to match the width of the paper, but do not lock it. Move the paper
support so it is midway between the sprocket units.
6. Make sure the paper has a clean, straight leading edge. Open the sprocket covers. Fit the
first holes of the paper over the sprocket pins and then close the sprocket covers.
7. Slide the right sprocket unit to remove any slack in the paper and lock it in place. Now
the paper is in the paper-park position.
PN 4237616B 3.3-5
8. To separate the incoming paper from the printed paper, attach the paper guide by
holding it horizontally and fitting its notches over the printer mounting posts
(Figure 3.3-10). Slide the paper guide towards the front of the printer until you feel it
click.
Figure 3.3-10 Replace Paper Guide
9. Slide the left and right paper edge guides (used for single sheets) to the center.
10. Close the printer cover (Figure 3.3-11).
Figure 3.3-11 Printer Ready for Continuous Feed Printing
11. Turn the printer ON.
CAUTION Risk of damage to the printer. Use the knob on the right side of the printer only to clear paper
jams and only when the printer is off. Otherwise, you may damage the printer or cause it to lose the
top-of-form position. Do not turn the printer knob unless the power is turned OFF.
12. Press the LF/FF button to feed the paper to the loading position.
13. Verify that the paper and the ribbon are not touching each other. If they are in direct
contact, the ribbon is not installed correctly. Reposition the ribbon between the print
head and ribbon guide (Figures 3.3-4 and 3.3-5).
3.3-6 PN 4237616B
Configure the Printer
LX300 Printer
ATTENTION: The LX300 printer must be set to Draft mode to ensure printouts, such as patient
result reports, are formatted correctly.
At the printer control panel (Figure 3.3-12), verify the printer is set to the Draft mode (the
LED above the number 2 is glowing). If the LED is not glowing, press the FONT key until it
does glow.
Figure 3.3-12 LX300 Printer Control Panel
When the printer receives data, it will begin printing automatically. For addional information,
see the printer user manual.
LX300+ Printer
At the printer control panel (Figure 3.3-13), press and hold the TEAR OFF button while
switching the printer On to access the parameter setup meni. Follow the printed instructions
to setup the printer. The printer control functions and default settings are shown in
Table 3.3-1 and Table 3.3-2.
Figure 3.3-13 LX300+ Printer Control Panel
Tear Off Tear Off LF/FF Load/Eject Paper Out

Pause
, H= BJ
Draft Condenced
Roman
Roman Condenced
S a n s S e r if
S a n s S e r if C o n d e n c e d
Font Micro Adjust 3sec
7616122B
Table 3.3-1 LX300+ Printer Controls and Indicators
Control/Indicator Function
LF/FF button r Feeds paper line by line when pressed and released.
r Ejects a single sheet or advances continuous paper to the next
top-of-form position when held down.
Load/Eject button r Loads a single sheet of paper.
r Ejects a single sheet of paper if a sheet is loaded.
r Loads continuous paper backwards to the standby position.
r Feeds continuous paper backwards to the standby position.
PN 4237616B 3.3-7
Table 3.3-1 LX300+ Printer Controls and Indicators (Continued)
Control/Indicator Function
Paper Out light r On when no paper is loaded in the selected paper source or paper is
not loaded correctly.
r Flashes when paper has not been fully ejected or a paper jam has
occurred.
Pause light r On when printer is paused.
r Flashes when the printer is in the Micro Adjust mode.
r Flashes when the print head has overheated.
Pause button r Stops printing temporarily and resumes printing when pressed
again.
When pressed for 3 seconds, turns on the Micro Adjust mode*. To
turn off the mode, press again.
Tear Off button† r Advances continuous paper to the tear-off position.
r Feeds continuous paper backward from the tear-off position to the
top-of-form position.
Tear Off lights† r Lights when continuous paper is in the tear-off position, otherwise
the lamps indicate the selected font.
* In the Micro Adjust mode, press the LF/FF and Load/Eject buttons to adjust the top-of-form
position.
† In Micro Adjust mode, press the Tear Off button to select the font to use for printing. The Tear
Off lights turn on, off or flash to indicate the selected font.
Table 3.3-2 LX300+ Printer Default Settings
Page length for tractor 6 inch

Skip over perforation Off
Auto tear Off Off
Auto line feed Off
Print direction Bi-D
Software ESC/P
0 slash 0
High speed draft On
I/F mode Auto
Auto I/F wait time 10 seconds
Baud rate 19200BPS
Parity None
Parallel I/F bidirectional mode On
Packet mode Auto
3.3-8 PN 4237616B
Table 3.3-2 LX300+ Printer Default Settings (Continued)
Character table PC 437

International character set for Italic table Italic U.S.A.
Manual feed wait time 1.5 seconds
Buzzer On
Auto CR (IBM 2380 Plus) Off
IBM character table Table2
Complete the Instrument Installation

Return to Heading 3.2, INITIAL SETUP and continue the installation starting at the Power
On the Instrument heading.
PN 4237616B 3.3-9
3.3-10 PN 4237616B
CONTENTS
4 SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1 GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY

ANALYZER, 4.1-1
General Guidelines, 4.1-1
Safety Precautions, 4.1-1
Accessibility, 4.1-1
Electronic Precautions, 4.1-1
Environment Protection, 4.1-1
Procedures, 4.1-1
Tools and Supplies, 4.1-2
Instrument Performance Verification, 4.1-2
Service Password, 4.1-2
User Mode, 4.1-2
How to Disable the Right Side Door Interlock, 4.1-2
How to Reactivate the Right Side Door Interlock, 4.1-3
Power Down / Power Up the Instrument, 4.1-3
Purpose, 4.1-3
Power Down, 4.1-3
Power Up, 4.1-4
Reset the Instrument, 4.1-4
System Reset Cycle, 4.1-4
Hardware Reset, 4.1-4
4.2 OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND

COVERS, 4.2-1
Purpose, 4.2-1
Opening the Right Side Door, 4.2-1
Bypassing the Right Side Door interlock, 4.2-1
Removing the Left Side Panel, 4.2-2
Removal, 4.2-2
Opening the Main Card Door, 4.2-3
Installation, 4.2-3
Removing the Rear Access Panel, 4.2-3
Removal, 4.2-4
Installation, 4.2-4
Removing the Top Cover, 4.2-4
Removal, 4.2-4
Installation, 4.2-5
Removing the Front Cover, 4.2-5
Removal, 4.2-5
Installation, 4.2-7
4.3 PREPARATION TO SHIP THE INSTRUMENT, 4.3-1

Purpose, 4.3-1
Bleach the Baths (20 minutes), 4.3-1
Clean the External Surfaces (20 minutes), 4.3-2
PN 4237616B 4-i
CONTENTS
Clean the Tubing and Chambers (60 minutes), 4.3-2

Preparation, 4.3-2
Cycle Routine, 4.3-3
Drain and Rinse, 4.3-3
4.4 FLOW CELL CHECKS AND ADJUSTMENTS, 4.4-1

Purpose, 4.4-1
Preparation, 4.4-1
Flow Cell Checks, 4.4-1
DIFF Lamp Voltage Adjustment, 4.4-3
Preparation, 4.4-3
Adjustment, 4.4-3
Resistive Channel Adjustment, 4.4-5
Preparation, 4.4-5
Adjustment, 4.4-6
Absorbance Channel Adjustment, 4.4-7
Preparation, 4.4-7
Adjustment, 4.4-7
Final Verification, 4.4-9
4.5 BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT, 4.5-1

Purpose, 4.5-1
Preparation, 4.5-1
Alignment Check, 4.5-1
Alignment Adjustment, 4.5-3
Verification, 4.5-4
4.6 SAMPLE PROBE CHECKS AND ADJUSTMENTS, 4.6-1

Purpose, 4.6-1
Sample Probe Checks, 4.6-2
Home Position Check, 4.6-2
Inside Bath Position Check, 4.6-3
Sample Probe Adjustments, 4.6-4
Home Position Adjustment, 4.6-4
Inside Bath Position Adjustment, 4.6-5
4.7 HGB BLANK ADJUSTMENT, 4.7-1

Purpose, 4.7-1
Preparation, 4.7-1
Adjustment, 4.7-2
Verification, 4.7-2
4-ii PN 4237616B
CONTENTS
4.8 APERTURE CURRENT CHECK, 4.8-1

Purpose, 4.8-1
Procedure, 4.8-1
4.9 RBC/PLT GAIN ADJUSTMENT, 4.9-1

Purpose, 4.9-1
Procedure, 4.9-1
Preparation, 4.9-1
Adjustments, 4.9-2
4.10 WBC/BASO GAIN ADJUSTMENT, 4.10-1

Purpose, 4.10-1
Procedure, 4.10-1
Preparation, 4.10-1
Adjustment, 4.10-2
4.11 DRAIN SENSOR ADJUSTMENT, 4.11-1

Purpose, 4.11-1
Preparation, 4.11-1
Adjustment, 4.11-2
Wrap Up, 4.11-2
4.12 TRANSFER SENSOR ADJUSTMENT, 4.12-1

Purpose, 4.12-1
Preparation, 4.12-1
Adjustment, 4.12-2
Wrap Up, 4.12-2
4.13 MOTOR CURRENT ADJUSTMENT, 4.13-1

Purpose, 4.13-1
Procedure, 4.13-1
4.14 THRESHOLD ADJUSTMENTS, 4.14-1

Purpose, 4.14-1
Procedure, 4.14-1
4.15 REAGENT TEMPERATURE CHECK AND ADJUSTMENT, 4.15-1

Purpose, 4.15-1
Reagent Temperature Check, 4.15-1
Reagent Temperature Adjustment, 4.15-3
Preparation, 4.15-3
Adjustment, 4.15-4
PN 4237616B 4-iii
CONTENTS
4.16 BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT, 4.16-1

Purpose, 4.16-1
Bath Enclosure Temperature Check, 4.16-1
Bath Enclosure Temperature Adjustment, 4.16-2
Preparation, 4.16-2
Adjustment, 4.16-3
4.17 VACUUM CHECKS AND ADJUSTMENTS, 4.17-1

Purpose, 4.17-1
Waste Syringe Vacuum Check, 4.17-1
Count Syringe Vacuum Check and Adjustment, 4.17-2
Count Syringe Vacuum Check, 4.17-2
Count Syringe Vacuum Adjustment, 4.17-3
4.18 MIX BUBBLE ADJUSTMENT, 4.18-1

Purpose, 4.18-1
Tools/Supplies Required, 4.18-1
Procedure, 4.18-1
4.19 HEATER ASSEMBLY REPLACEMENT, 4.19-1

Purpose, 4.19-1
Removal, 4.19-1
4.20 POWER SUPPLY REPLACEMENT, 4.20-1

Purpose, 4.20-1
Removal, 4.20-1
4.21 START SWITCH REPLACEMENT, 4.21-1

Purpose, 4.21-1
Removal, 4.21-1
4.22 OPTICAL BENCH DISASSEMBLY AND REPLACEMENT, 4.22-1

Purpose, 4.22-1
Removal, 4.22-1
4-iv PN 4237616B
CONTENTS
4.23 BAR-CODE READER TESTING AND CONFIGURATION, 4.23-1

Purpose, 4.23-1
Read Test, 4.23-1
Default Settings, 4.23-2
Codabar - Start/Stop Equality Check/Output, 4.23-4
Interleaved 2-of-5 Options, 4.23-5
4.24 REAGENT SYRINGES ASSEMBLY REPLACEMENTS, 4.24-1

Purpose, 4.24-1
Preparation, 4.24-1
Removal, 4.24-2
O-Ring, Washer, and Piston Replacement, 4.24-2
4.25 COUNT SYRINGE COMPONENT REPLACEMENTS, 4.25-1

Purpose, 4.25-1
Preparation, 4.25-1
Removal, 4.25-2
O-ring, Washer, and Piston Replacement, 4.25-5
4.26 SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT

REPLACEMENTS, 4.26-1
Purpose, 4.26-1
Preparation, 4.26-1
Removal, 4.26-1
Sample Probe Replacement, 4.26-2
Rinse Block Assembly Component Replacement, 4.26-2
4.27 WASTE SYRINGE COMPONENT REPLACEMENTS, 4.27-1

Purpose, 4.27-1
Preparation, 4.27-1
Removal, 4.27-2
PN 4237616B 4-v
CONTENTS
4.28 CLEANING THE BATH ENCLOSURE, 4.28-1

Purpose, 4.28-1
Procedure, 4.28-1
4.29 5diff SYRINGE ASSEMBLY REPLACEMENTS, 4.29-1

Purpose, 4.29-1
Preparation, 4.29-1
Removal, 4.29-1
4.30 FLOW CELL COAXIAL CABLE REPLACEMENT, 4.30-1

Purpose, 4.30-1
Preparation, 4.30-1
Removal, 4.30-1
Replacement, 4.30-4
4.31 OPTICAL BENCH LAMP REPLACEMENT, 4.31-1

Purpose, 4.31-1
Preparation, 4.31-1
Removal, 4.31-3
Lamp Replacement, 4.31-4
4.32 DILUENT RESERVOIR REPLACEMENTS, 4.32-1

Purpose, 4.32-1
Preparation, 4.32-1
Removal, 4.32-1
O-ring and Washer Replacement, 4.32-2
4.33 SAMPLE SYRINGE ASSEMBLY REPLACEMENTS, 4.33-1

Purpose, 4.33-1
Preparation, 4.33-1
Removal, 4.33-1
4-vi PN 4237616B
CONTENTS
4.34 DRAINING BATH REPLACEMENTS, 4.34-1

Purpose, 4.34-1
Preparation, 4.34-1
4.35 O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and WBC/BASO

Baths), 4.35-1
Purpose, 4.35-1
Preparation, 4.35-1
Removal, 4.35-2
Replacing the Coaxial Cable O-ring on the Bath Electrode, 4.35-3
Replacing the Aperture O-rings in the Counting Head, 4.35-5
Align the Bath Assembly, 4.35-8
4.36 OPTICAL BENCH PRELIMINARY ADJUSTMENTS, 4.36-1

Purpose, 4.36-1
Preparation, 4.36-1
Verify the Flow Cell is Free of Bubbles, 4.36-2
Course Adjustments, 4.36-2
Y-Axis Adjustment, 4.36-2
X-Axis Adjustment, 4.36-3
Lamp Alignment, 4.36-5
4.37 FLOW CELL WBC BALANCE, 4.37-1

Purpose, 4.37-1
Procedure, 4.37-1
4.38 SETTING diff+/diff- THRESHOLDS, 4.38-1

Purpose, 4.38-1
Procedure, 4.38-1
4.39 MAIN CARD REPLACEMENT AND SOFTWARE TRANSFER, 4.39-1

Purpose, 4.39-1
Preliminary Setup, 4.39-1
Removing Main Card, 4.39-1
Transferring EPROMs to Replacement Card, 4.39-3
Installing Main Card, 4.39-4
Verifying System Configuration, 4.39-5
Patient Ranges, Action Ranges and Thresholds, 4.39-6
Verifying Main Card Settings, 4.39-7
PN 4237616B 4-vii
CONTENTS
ILLUSTRATIONS
4.1-1 Location of the Power On/Off Rocker Switch, 4.1-3
4.2-1 Opening the Right Side Door, 4.2-1
4.2-2 Removing the Left Side Panel, 4.2-3
4.2-4 Top Cover - Side Screw Locations, 4.2-5
4.2-5 Reagent Compartment Screw Locations, 4.2-6
4.2-6 Torx Screw Location Inside Front Panel, 4.2-6
4.2-7 Torx Screw Location on Right Frame, 4.2-6
4.2-8 Front Panel Screw Locations, 4.2-7
4.2-9 Connector for the Keypad and LCD Card, 4.2-7
4.4-1 Diff Adjustment Screen, 4.4-2
4.4-2 Potentiometer R11 - Location on the Optical Bench Assembly, 4.4-3
4.4-3 Potentiometer R11 Location - Top View from the Front of the Instrument, 4.4-3
4.4-4 Main Card Flow Cell Adjustments, 4.4-5
4.4-5 Front Adjustment Knob - Optical Bench, 4.4-7
4.4-6 Side Adjustment Screw - Optical Bench, 4.4-7
4.5-2 Close-up of Probe Position, 4.5-2
4.5-3 Sample Probe at the WBC/BASO Bath, 4.5-2
4.5-4 Close-up of Probe at the WBC/BASO Bath, 4.5-2
4.5-5 Location of Screws Securing the Baths Support Panel, 4.5-3
4.5-8 Sample Probe Position, Right Side, 4.5-4
4.6-1 Dilution Screen, 4.6-1
4.6-2 Acceptable Distance between the Sample Probe Tip and the Traverse, 4.6-2
4.6-3 Measuring the Distance between the Sample Probe Tip and the Traverse, 4.6-2
4.6-4 Correct View through the Port, 4.6-3
4.6-5 Incorrect View through the Port, 4.6-3
4.6-6 Properly Adjusted Sample Probe Tip, 4.6-4
4.6-7 Ideal Probe Position, 4.6-5
4.6-8 Improper Probe Tip Position - Too Forward, 4.6-5
4.6-9 Improper Probe Tip Position - Too Backward, 4.6-5
4.6-10 Improper Probe Tip Position - Too High, 4.6-6
4.6-11 Improper Probe Tip Position - Too Low, 4.6-6
4.7-1 Main Card Hgb Blank Adjustment, 4.7-1
4.8-1 LMNE CIS, GR (RBC), and GB (WBC) Coax Locations, 4.8-1
4.9-1 Main Card RBC/PLT Gain Adjustments, 4.9-1
4.10-1 Main Card WBC/BASO Gain Adjustment, 4.10-1
4.11-1 Main Card Drain Sensor Adjustment, 4.11-1
4.12-1 Main Card Transfer Sensor Adjustment, 4.12-1
4.13-1 Main Card Motor Current Adjustments, 4.13-2
4.14-1 Main Card Threshold Adjustments, 4.14-2
4.15-1 Thermometer Probe inside the DIFF Bath, 4.15-1
4.15-2 Main Card Heating Status LED Location, 4.15-2
4-viii PN 4237616B
CONTENTS
4.15-3 Location of the Label Containing the Temperature Value for the Heater
Assembly, 4.15-3
4.16-1 Temperature Sensor Location - View with the Right Side Door Open, 4.16-1
4.16-2 Location of the Label Containing the Temperature Value for the Temperature
Sensor, 4.16-2
4.17-1 Attach the Vacuum Meter to the Waste Syringe, 4.17-1
4.17-2 Attach the Vacuum Meter to the Count Syringe, 4.17-2
4.19-1 Captive Hex Screw Loactions - Reagent Syringes and 5diff Syringe, 4.19-1
4.19-2 CHC M3 x 6 Screw Locations, 4.19-2
4.19-3 Heater Assembly, 4.19-3
4.19-5 Main Card Heater Assembly Replacement, 4.19-4
4.19-6 Heater Assembly After Removal, 4.19-5
4.19-8 Heater Assembly - Orientation Inside the Instrument, 4.19-6
4.19-9 Heater Assembly - Port Locations, 4.19-6
4.19-10Baths Assembly Support Panel Nut Locations, 4.19-7
4.19-11Location of Openings in the Baths Assembly Support Panel, 4.19-7
4.19-12Heater Assembly Screw Locations, 4.19-8
4.20-3 Optical Module - Lamp Supply Cable Location, 4.20-2
4.20-4 Location of Screws Securing the Power Supply to the Rear Panel, 4.20-3
4.20-5 Screws Securing the Power Supply to the Instrument Frame, 4.20-3
4.20-6 Main Card Heater Assembly Replacement Adjustment, 4.20-4
4.21-1 Fan Removal - Right Side Compartment, 4.21-1
4.21-2 Start Switch Screw Locations - With Fan Removed, 4.21-2
4.21-3 Disconnected Start Switch - Front View with Front Panel Removed, 4.21-2
4.21-4 Start Switch Orientation, 4.21-3
4.22-3 Disconnection Sites for Named Components, 4.22-2
4.22-4 Captive Screw Locations - Optical Bench Assembly, 4.22-3
4.22-5 Connection Sites for Named Components, 4.22-4
4.24-2 O-rings and Washers - Reagent Syringes Assembly, 4.24-3
4.24-3 Bottom Plate Screw Locations and Tightening Patterns, 4.24-4
4.25-2 Motor Interconnect Card - Count Syringe Connector Locations, 4.25-2
4.25-3 Count Syringe - Ground Wire Location, 4.25-3
4.25-5 Count Syringe - Captive Screw Locations, 4.25-4
4.25-6 Count Syringe - Piston, O-ring, and Washer Replacement, 4.25-5
4.25-7 Count Syringe - O-ring and Washer Replacement, 4.25-6
4.26-1 Probe Rinse Block Screw Locations, 4.26-1
4.26-2 Lift the Sample Probe Lock-Lever, 4.26-2
PN 4237616B 4-ix
CONTENTS
4.26-3 Remove Probe and Rinse Block Assembly, 4.26-2

4.26-4 Reassemble the Rinse Block Assembly, 4.26-3
4.27-2 Motor Interconnect Card - Waste Syringe Connector Locations, 4.27-2
4.27-3 Waste Syringe - Tie Wrap and Ground Wire Location, 4.27-3
4.27-4 Waste Syringe - Captive Screw Locations, 4.27-4
4.27-5 Waste Syringe - Piston, O-ring, and Washer Replacement, 4.27-5
4.27-6 Waste Syringe - O-ring and Washer Replacement, 4.27-6
4.29-2 Valve and Tubing Locations - Left Side View, 4.29-2
4.29-3 Screw Locations - 5diff Syringe, 4.29-3
4.29-4 Bottom Plate - 5diff Syringe, 4.29-3
4.29-5 Bottom Plate and Attachments - Housing Removed, 4.29-4
4.29-6 Illustrated Parts - 5diff Syringe, 4.29-5
4.30-2 T-Connector Location, 4.30-2
4.30-3 Disconnecting the Isolator Chamber from the T-connector, 4.30-2
4.30-4 Coaxial Cable Connector - Top View, 4.30-3
4.30-5 Flow Cell Screw Locations - Top View, 4.30-3
4.30-6 Ground Screw Removal, 4.30-4
4.30-7 Connector Location - Top View, 4.30-5
4.31-1 Optical Bench Lamp Assembly, 4.31-2
4.31-2 Power Connector Location - Top View, 4.31-3
4.31-3 Screw Locations - Lamp Housing, 4.31-3
4.31-4 Winged Metal Bracket - Top View, 4.31-4
4.32-1 Diluent Reservoir Screw Locations, 4.32-1
4.32-2 Diluent Reservior O-ring and Washer Positioning, 4.32-2
4.33-1 Disconnect Tubing at Valve 18, Port 2, 4.33-1
4.33-2 Screw Locations - Sample Syringe Housing, 4.33-2
4.33-3 Sample Syringe Output Port, 4.33-2
4.33-4 Screw Locations on the Bottom of the Sample Syringe Housing, 4.33-3
4.33-5 Illustrated Parts - Sample Syringe, 4.33-4
4.34-1 Removing a Draining Bath, 4.34-1
4.34-2 Draining Bath O-ring Placement, 4.34-2
4.34-3 Draining Bath O-Ring Locations, 4.34-2
4.35-2 Close-up of Probe Positioning, 4.35-2
4.35-3 Baths Assembly Screw Locations, 4.35-3
4.35-4 Electrode Screw Locations, 4.35-3
4.35-5 Removing the Coaxial Cable O-ring from the Bath Electrode, 4.35-4
4.35-6 Making a Protective Cover from a Micropipette Tip, 4.35-4
4.35-7 Bath Electrode Locations, 4.35-5
4.35-8 Location of the Aperture and Its Two O-Rings, 4.35-6
4.35-11Sample Probe Position, Right Side, 4.35-9
4.36-2 Checking the Lens to Flow Cell Gap, 4.36-2
4-x PN 4237616B
CONTENTS
4.36-3 Front Knob for Y-Axis Adjustment, 4.36-3

4.36-5 How to Position the White Paper, 4.36-4
4.36-8 Lamp Adjustment Screw, 4.36-5
4.36-10Side Screw - X-Axis Adjustment, 4.36-6
4.37-1 WBC/Flow Cell Balance Screen, 4.37-1
4.38-1 diff Flag Sensitivity Screen, 4.38-1
4.39-1 Main Card - Connections and EPROMs, 4.39-2
4.39-2 Main Card - Potentiometers /Test Points, 4.39-9
TABLES
4.13-1 Motor Voltage Limits, 4.13-1
4.14-1 Threshold Voltage Limits, 4.14-1
4.18-1 Mixing Bubble Limits, 4.18-1
4.20-1 Power Supply Voltages, 4.20-4
4.23-1 Test Labels With Check Digit (Checksum), 4.23-1
4.23-2 Test Labels Without Check Digit, 4.23-2
4.23-3 Bar-code Labels for Default Configuration, 4.23-3
4.39-1 Main Card - Plug/Jack Connections, 4.39-3
4.39-2 AC•T 5diff Menu Paths - System Settings, 4.39-5
4.39-3 AcT 5diff - Main Card Settings, 4.39-7
PN 4237616B 4-xi
CONTENTS
4-xii PN 4237616B
4SERVICE AND REPAIR PROCEDURES 4
4.1 GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY ANALYZER
General Guidelines
Safety Precautions
Review and heed the general safety warnings and cautions listed under Heading 1.2, SAFETY
PRECAUTIONS.
Accessibility
Ensure there is adequate space to work and to access the instrument components safely.
Electronic Precautions
CAUTION Risk of damage to electronic components. If the power is ON while removing or replacing
electronic components, the instrument could be damaged. To prevent damage to electronic components,
always be sure power is OFF before removing or replacing printed circuit cards and components.
Before disconnecting or reconnecting any electronic component, turn the instrument off and
disconnect the power cord from the instrument or the wall outlet. See Power Down / Power
Up the Instrument in this section.
Environment Protection
If the AC•T 5diff analyzer is old and ready for disposal, the instrument and its accessories
must be collected by a company that specializes in the elimination or the recycling of
laboratory equipment according to the legislation.
Procedures
the instrument with the doors or panels open, you may become injured or contaminated. To prevent
possible injury or biological contamination, you must wear appropriate safety glasses, a lab coat, and
gloves when servicing this instrument with the doors or panels open.
Adjustment and maintenance procedures that need to be done on the AC•T 5diff hematology
analyzer are in this section, including:
r Hydraulic maintenance and adjustments.

r Pneumatic maintenance and adjustments.
r Power supply maintenance and adjustments.
r Electronic maintenance and adjustments.
Note: Read each procedure entirely before beginning the service or repair.
PN 4237616B 4.1-1
SERVICE AND REPAIR PROCEDURES
GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY ANALYZER
Tools and Supplies

You can do most procedures using the standard Service Tool Kit and a digital voltmeter
(DVM). Any special tools, supplies, or equipment required are identified under the
Tools/Supplies Needed heading at the beginning of the procedure.
Instrument Performance Verification

When a service/repair procedure requires some type of instrument performance verification
upon completion, a Verification heading is provided with the necessary steps that must be
completed.
Service Password
CAUTION Risk of instrument damage. Do not disclose the Service password to a Customer. An untrained
person with access to the Service menu may activate routines in an unauthorized order which may result in
damage to critical systems such as the sample probe.
When performing some of the service and maintenance procedures, it will be necessary to use
the Service menu. Access to this Service menu requires a password (which is actually a
number) 239. The sequence for accessing the Service menu is:
1. From the Main Menu, select 4. DIAGNOSTICS tt 5. SERVICE. The SERVICE PASSWORD
prompt appears.
2. At the numeric keypad, press [2] [3] [9] then ENTER. The Service menu appears.
3. From the service menu, select the desired option.
User Mode
How to Disable the Right Side Door Interlock
WARNING Risk of personal injury. When SERVICE is selected as the User mode, the right side door
interlock is bypassed allowing instrument operation with the right side door open. Avoid any unnecessary
contact with the sample probe. The probe is sharp and may contain biohazardous materials. When the
service call is complete, make sure the interlock is reactivated to ensure the Customer is not accidently
injured by the sample probe or its movement.
SERVICE must be the selected User mode to operate the instrument with the right side door
open. When the instrument is set to the Service mode, the interlock for the right side door is
bypassed. To set the User mode to service:
1. From the Service menu, select 9. OTHERS tt 1. USER MODE.

2. At the instrument keypad,
a. Press the down arrow key to move the blinking cursor to the SERVICE box.
b. Press the decimal point key. Make sure a dot appears inside the box.
Note: If you press the decimal point too long, the dot may disappear. The dot inside
the SERVICE box indicates the right side door interlock is bypassed.
c. Press ESC to exit the User Mode screen.
d. Press ESC as many times as necessary to return to the desired menu.
4.1-2 PN 4237616B
GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY ANALYZER 4
How to Reactivate the Right Side Door Interlock
When the service call is complete, make sure the right side door interlock is reactivated to
ensure the Customer is not accidently injured by the sample probe or its movement. To
reactivate the right side door interlock:

b. Press the decimal point key or the DEL key. Make sure the dot no longer appears
inside the SERVICE box. The right side door interlock is reactivated.
Note: If you press the decimal point too long, the dot may reappear. The dot inside
c. Press ESC as many times as necessary to return to the Main Menu.
Power Down / Power Up the Instrument
Purpose
Following the Power Down procedure ensures all power is removed from the instrument,
preventing personal injury from electronic shock.
Power Down
1. Switch the Power On/Off rocker switch from ON (-) to OFF (O). This rocker switch is
located at the base of the left side panel.
Figure 4.1-1 Location of the Power On/Off Rocker Switch
2. Unplug the ac power cord. Either remove the cord from the instrument (at the back
panel, in the lower right corner) or from the ac wall outlet.
PN 4237616B 4.1-3
GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY ANALYZER
Power Up
1. Plug the ac power cord into the instrument (at the back panel, in the lower right corner)
or the ac wall outlet, as applicable.
2. Switch the Power On/Off rocker switch from OFF (O) to ON (-). This rocker switch is
located at the base of the left side panel.
Note: An automatic Startup routine and background check is performed. If MANUAL
STARTUP is the selected User Mode, an automatic Startup and background check does
not occur. To initiate a Startup routine and background check, you must press the
STARTUP button on the instrument keypad.
Reset the Instrument

Two instrument resets are available: System Reset Cycle and Hardware Reset.
System Reset Cycle

From the Main Menu, selecting 4. DIAGNOSTICS tt 1. SYSTEM RESET CYCLE initiates the
following actions:
r Checks all stepper motor positions

r Checks all sensors
r Drains and rinses the baths
r Cleans the sample probe
r Checks reagent volumes
r Fills the diluent reservoir
r Checks all mechanical functions
Hardware Reset
From the Main Menu, selecting 4. DIAGNOSTICS tt 4. HARDWARE SYSTEMS tt
1. HARDWARE RESET moves all stepper motors to their home position.
4.1-4 PN 4237616B
OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND COVERS 4
4.2 OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND COVERS
Purpose
Use the procedures in this section for accessing instrument components. This access includes:
r Opening the Right Side Door
r Removing the Left Side Panel
r Removing the Rear Access Panel
r Removing the Top Cover
r Removing the Front Cover
Tools/Supplies Needed
B Allen wrenches, 2.5 mm and 3 mm
B Screwdriver, T10 torx
B Locker key (provided)
Opening the Right Side Door

The pneumatic access door on the right side of the instrument allows the operator to access
hydraulic assemblies for maintenance operations. This area is also called the bath enclosure.
It is mandatory to keep the door locked during a cycle to ensure proper heating of the
dilutions inside the baths. The door is secured using two captive screws that require a special
key.
To open the door, place the edge of the key inside the screw slot and turn the captive screw
counterclockwise (Figure 4.2-1).
Figure 4.2-1 Opening the Right Side Door
Bypassing the Right Side Door interlock
PN 4237616B 4.2-1
OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND COVERS
SERVICE must be the selected User mode to operate the instrument with the right side door
open. When the instrument is set to the Service mode, the interlock for the right side door is
bypassed. To set the User mode to service:

Note: If you press the decimal point too long, the dot may disappear. The dot inside
c. Press ESC to exit the User Mode screen.
d. Press ESC as many times as necessary to return to the desired menu.
3. When the service call is complete, make sure the right side door interlock is reactivated
to ensure the Customer is not accidently injured by the sample probe or its movement.
To reactivate the right side door interlock:
a. From the Service menu, select 9. OTHERS tt 1. USER MODE.
b. At the instrument keypad,
1) Press the down arrow key to move the blinking cursor to the SERVICE box.
2) Press the decimal point key or the DEL key. Make sure the dot no longer appears
inside the SERVICE box. The right side door interlock is reactivated.
Note: If you press the decimal point too long, the dot may reappear. The dot
inside the SERVICE box indicates the right side door interlock is bypassed.
3) Press ESC as many times as necessary to return to the Main Menu.
Removing the Left Side Panel
Removal
Remove the left side panel to gain access to the Main card:
1. Turn the instrument off and unplug the power cord from the instrument or the wall.
2. Remove the left side panel (Figure 4.2-2). Use a 3 mm hex key to remove the four hex
screws securing the panel to the instrument frame.
3. Open the Main card door and carefully anchor the door behind the white plastic catch to
keep it open.
4.2-2 PN 4237616B
Figure 4.2-2 Removing the Left Side Panel
Opening the Main Card Door

Opening the Main card door allows the operator to access hydraulic assemblies for
maintenance operations.
1. To the right of the Main card, turn the two captive knobs counterclockwise to release the
Main card door.
CAUTION A flat cable is connected to the rear of the Main card. Be careful when you open the door (where
the Main card is attached) that you do not disconnect or damage this cable.
2. When opening the Main card door, carefully anchor the door behind the white plastic
catch to keep it open.
Installation
1. Lift the white plastic catch to release the Main card.
2. Close the Main card door and turn the two captive knobs clockwise to secure it.
3. Replace the left side panel and install the four hex screws removed earlier.
4. Reconnect the power cord.
Removing the Rear Access Panel

The back panel provides access to the motors and sensor connectors on the Motor
Interconnect card.
PN 4237616B 4.2-3
Removal
Use a 3.0 mm Allen key to remove the six hex screws securing the panel to the back of the
instrument (Figure 4.2-3). Set the panel aside.
Figure 4.2-3 Rear Access Panel Screw Locations
Alignment
holes
Hex
7653001A
screws (6)
Installation
This panel is not reversible, When replacing this panel, make sure the center opening at the
top of the panel and the opening at the bottom of the panel are aligned as shown in
Figure 4.2-3.
Removing the Top Cover

The top cover is secured to the instrument frame with five hex screws; three screws at the
back of the instrument and one hex screw on each side of the instrument. The right door
must be opened and the left panel must be removed before removing the top cover.
Removal
2. Remove the left side panel. See Removing the Left Side Panel in this section.
3. In the left side compartment, remove the hex screw in the front upper corner
(Figure 4.2-4).
4.2-4 PN 4237616B
Figure 4.2-4 Top Cover - Side Screw Locations
4. Open the right side door. See Opening the Right Side Door in this section.
5. In the right side compartment, remove the hex screw in the front upper corner
(Figure 4.2-4).
6. At the rear of the instrument, remove the three hex screws securing the top cover to the
instrument frame (Figure 4.2-4).
7. Carefully slide the cover back and off the instrument.
8. Set the cover aside.
Installation
1. Carefully position the top cover back on the instrument.
2. In the left side compartment, replace the hex screw in the front upper corner.
3. Open the right side door and replace the hex screw in the front upper corner.
4. At the rear of the instrument, replace the three hex screws that secure the top cover to
the instrument frame.
6. Close the right side door.
Removing the Front Cover

The front cover requires that the right side door be opened, and the left side panel and top
cover be removed.
Removal
1. Turn the instrument off and disconnect the power cord from the instrument or the wall.
2. Remove the top cover from the instrument. See Removing the Front Cover in this
section.
PN 4237616B 4.2-5
3. Open the reagent door and remove the four bottles.

4. Disconnect the tubing from the stopper.
CAUTION Risk of damage to the reagent compartment door. The two left screws inside the reagent
compartment not only secure the compartment to the instrument frame but also secure the reagent
compartment door. When these screws are removed, the door may fall. If the door becomes bent, it may
not close properly. When removing the two left screws, hold the reagent compartment door securely to
prevent it from falling when it detaches.
5. Unscrew the four hex screws shown in Figure 4.2-5. When removing the two left screws,
hold the reagent compartment door securely to prevent it from falling when it detaches
from the instrument.
Figure 4.2-5 Reagent Compartment Screw Locations
6. Make sure the sample probe is inside its housing then push the sample probe housing
towards the back of the instrument.
7. Unscrew several turns the two torx screws shown in Figures 4.2-6 and 4.2-7.
Figure 4.2-6 Torx Screw Location Inside Front Panel Figure 4.2-7 Torx Screw Location on Right Frame
4.2-6 PN 4237616B
8. Unscrew the two hex screws shown in Figure 4.2-8.
Figure 4.2-8 Front Panel Screw Locations
9. Remove the flat connector attached to Keypad and LCD card (Figure 4.2-9).
Figure 4.2-9 Connector for the Keypad and LCD Card
10. Lift the front cover up and away slightly until the torx screws clear their holes then lift
the cover off the instrument.
11. Set the cover aside in a safe place where it will not get damaged.
Installation
1. Position the front cover back on the instrument. Make sure the holes for the two torx
screws are aligned with the screws that remained in the instrument.
2. Reattach the flat connector attached to Keypad and LCD card (Figure 4.2-9).
3. Replace the two hex screws located at the top inside the instrument (Figure 4.2-8).
4. Tighten the two torx screws shown in Figures 4.2-6 and 4.2-7.
5. Open the reagent door replace the four hex screws (Figure 4.2-5).
6. Reconnect the reagent tubing to its stopper and place the bottle back inside the reagent
compartment (match the color coding on the bottle label with the label on the panel).
PN 4237616B 4.2-7
7. Replace the top cover. Under Removing the Front Cover, see the installation
instructions.
4.2-8 PN 4237616B
PREPARATION TO SHIP THE INSTRUMENT 4
4.3 PREPARATION TO SHIP THE INSTRUMENT
Purpose
Use this procedure to clean and properly prepare the instrument for shipping.
B Fungicidal, bactericidal, virus killing detergent spray, non-corrosive for metals,
non-plastic altering
B High quality, fragrance-free bleach (10-12% sodium hypochlorite - available chloride)
B Distilled water
B Absorbent paper
B Two 500 mL glass beakers or flasks
Bleach the Baths (20 minutes)

1. If OFF, turn the instrument ON.
2. From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEMS tt 4. EXTENDED
CLEANING. The Extended Cleaning prompt appears.
3. Press ENTER and the message CYCLE IN PROGRESS. PLEASE WAIT. . . appears.
WARNING Risk of contamination. If you do not properly shield yourself while decontaminating the
instrument, you may become contaminated. To prevent possible biological contamination, you must wear
appropriate safety glasses, a lab coat, and gloves when performing this procedure.
4. When the message POUR 3 mL OF EXTENDED CLEANING REAGENTS INTO BATHS

appears,
a. Open the right side door.
b. Pour 3 mL of high quality, fragrance-free bleach (10-12% sodium hypochlorite -
available chloride) into each bath.
c. Close the right side door.
d. Press ENTER to continue. Allow the instrument to complete the cleaning procedure.
It takes about five minutes for the cycle to complete. The system will automatically
flush to remove the bleach poured in the baths earlier.
5. When the cycle is finished, turn the instrument OFF and unplug the power cord from
the instrument or the wall.
6. Open the right side door.
7. Spray the bactericidal cleaner on all biohazard areas and wait for 10 minutes (assemblies
in contact with the biohazard materials such as instrument cover, tube holder, keypad,
start key, assemblies close to the sample probe, and so forth).
PN 4237616B 4.3-1
PREPARATION TO SHIP THE INSTRUMENT
Clean the External Surfaces (20 minutes)

1. Make a 1:5 bleach solution: 4 parts distilled water to 1 part high quality, fragrance-free
bleach (10-12% sodium hypochlorite - available chloride).
2. Clean the work area around the instrument.
ATTENTION: Do not use a sponge or cloth to clean instrument assemblies. Use absorbent paper
towels that can be discarded in a biohazard container. For small or sensitive assemblies, use
lint-free tissues.
3. Use a spray detergent to remove blood stains and salt marks from the following
assemblies:
r Outer surfaces of the instrument (covers, LCD, reagent locations)
r Keypad
r Waste connector plug
r Liquid valve push button
r Assemblies close to the sample probe
r Tube holder assembly
r Overflow trays
ATTENTION: Do not bleach stainless steel components if the ambient room temperature is more
than 30° C (86° F).
4. Disinfect, with the diluted bleach solution, all assemblies that have been in contact with
biohazard materials.
5. Reinstall all the assemblies and set the instrument back to its initial configuration.
Clean the Tubing and Chambers (60 minutes)
Preparation
1. Locate two containers such as a glass beaker or flask that will hold a little more than
500 mL of liquid. Select containers that can be placed in front of the reagent
compartment when the front door is open.
a. In one container, prepare approximately 500 mL of a 1:10 bleach solution: 9 parts
distilled water to 1 part high quality, fragrance-free bleach (10-12% sodium
hypochlorite - available chloride).
b. Pour 500 mL of distilled water into the second container.
2. Turn the instrument ON.
3. Remove each pickup tube from its reagent container and place the tube in the diluted
bleach. Don’t forget the diluent pickup tube.
4. At the Main Menu, select 3. REAGENTS tt 3. PRIME tt 6. ALL REAGENTS to pull the diluted
bleach into the instrument.
5. Fill a glass or plastic tube with a 1:5 bleach solution: 4 parts distilled water to 1 part high
quality, fragrance-free bleach (10-12% sodium hypochlorite - available chloride).
4.3-2 PN 4237616B
PREPARATION TO SHIP THE INSTRUMENT 4
Cycle Routine
1. From the Service menu, select 7. BURN-IN.
2. Set the number of burn-in cycles to 15.
3. Cycle the 1:5 bleach solution to initiate the burn-in function. The instrument will cycle
15 times. Do not attempt to stop the cycles. Let the instrument operate until it stops.
Drain and Rinse

1. Remove the reagent pickup tube assemblies from the diluted bleach and wrap the tubes
in absorbent paper.
2. From the Main Menu, select 3. REAGENTS tt 3. PRIME tt 6. ALL REAGENTS to drain the
diluted bleach from the system.
3. When the prime cycles are complete, select 6. ALL REAGENTS again to ensure the bleach is
removed from the system.
4. Exchange the diluted bleach container with the vessel containing distilled water and
place the pickup tubes in the distilled water.
5. From the Prime menu, select 6. ALL REAGENTS to rinse the system with distilled water.
6. Remove the reagent pickup tubes from the distilled water and wrap the tubes in
absorbent paper.
7. From the Prime menu, select 6. ALL REAGENTS to drain the distilled water from the
system.
8. When the prime cycles are complete, select 6. ALL REAGENTS again to ensure the distilled
water is removed from the system.
WARNING When this process is complete, open the right side door and verify the DIFF bath is empty.
9. Turn the instrument OFF.

10. Remove the diluent input tubing (Figure 4.3-1, item 2).
Figure 4.3-1 Rear Panel Connections
PN 4237616B 4.3-3
PREPARATION TO SHIP THE INSTRUMENT
WARNING Risk of contamination. If you do not properly shield yourself while removing the waste tubing,
you may become contaminated. To prevent possible biological contamination, you must wear appropriate
safety glasses, a lab coat, and gloves when removing this tubing.
11. Remove the waste output tubing (Figure 4.3-1, item 1).
12. Disconnect any cables attached to the back of the instrument including the:
a. Bar-code reader connector, if attached (Figure 4.3-1, item 4).
b. Printer connector (Figure 4.3-1, item 5).
c. RS232C output, if attached (Figure 4.3-1, item 6).
d. Power supply cable (Figure 4.3-1, item 7).
13. Verify that all four reagent bottles are removed from the reagent compartment.
14. Close all the instrument doors.
15. Pack the instrument in its original box, if available.
4.3-4 PN 4237616B
FLOW CELL CHECKS AND ADJUSTMENTS 4
4.4 FLOW CELL CHECKS AND ADJUSTMENTS
Purpose
Use this adjustment procedure when the flow cell is moved or replaced, the DIFF tubing is
changed, or the DiffPlot does not look correct. No adjustment should be required when the
DIFF lamp is replaced.
To adjust the optical bench assembly, 5 µL of RBC/PLT latex is aspirated and diluted in the
DIFF bath with 2 mL of diluent. This dilution is then injected into the flow cell. Once the Diff
Adjustment screen appears, measurements are continuously displayed for 27 seconds with
updates occurring every 700 microseconds. Adjustments may be made during this 27 seconds
as needed. Three audible beeps indicate the end of the adjustment period. Do not make
adjustments once these beeps are heard. The screen is no longer being updated.
B Allen wrench, 3 mm
B Plastic potentiometer adjustment tool, PN 5415364
B RBC/PLT latex, PN - LAD002AS
B Several fresh whole-blood specimens
Preparation
1. If off, turn the instrument on.
2. From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEM tt 2. RINSE tt
2. FLOWCELL to remove air bubbles clinging to the inner optical surfaces.
3. Press ESC twice to return to the Diagnostics menu then select 5. SERVICE.
Flow Cell Checks

1. From the Service menu, select 2. MEASUREMENT tt 5. DIFF ADJUSTMENT.
2. When the DIFF ADJUSTMENT prompt appears, press ENTER to continue.
IMPORTANT Risk of misleading results. The RBC/PLT latex particles tend to clump as they settle out of
solution. Clumped latex particles will affect adjustment results. Mix the RBC/PLT latex vigorously before
use. A vortex may be used. Remix the latex thoroughly before each sampling.
3. Mix the RBC/PLT latex vigorously. Use a vortex, if available.

4. When the PLEASE SAMPLE LATEX prompt appears, present the vial of well-mixed latex
particles to the probe and press the aspirate switch to initiate aspiration and the routine.
5. When the Diff Adjustment screen appears (Figure 4.4-1), verify each result is acceptable
according to the following criteria:
PN 4237616B 4.4-1
FLOW CELL CHECKS AND ADJUSTMENTS
Figure 4.4-1 Diff Adjustment Screen
DIFF ADJUSTMENT 1/ 27 / 00 16:05 PM

DIFF LAMP
6.20 VOLTS
4.00 8.00
TRANSFER TIME
212
100 300
RESISTIVE CHANNEL
50
5 70
ABSORBANCE CHANNEL
180
10 240
CYCLE IN PROGRESS...
56%
6. Verify the DIFF LAMP value displayed on the screen is between 5.50 and 6.50.
r If the value on the screen is within the acceptable range, go to step 7.
r If the value on the screen is outside this acceptable range or or if you desire a value
closer to the target value (6.0), go to the DIFF Lamp Voltage Adjustment heading.
7. Verify the TRANSFER TIME value is between 150 and 250.
r If the TRANSFER TIME value is outside the acceptable range, there is a problem.
Complete the instructions under Heading 4.36, OPTICAL BENCH PRELIMINARY
ADJUSTMENTS before proceeding.
Note: When cells are not optically detected, the TRANSFER TIME value displayed
on the screen is 100 and backlighted. The backlighted number indicates the value is
less than 100, and may actually be zero. If the flow cell is so far out of alignment
that no cells are detected, the procedure under Heading 4.36 provides a series of
preliminary adjustments for the flow cell and optics lamp.
When the TRANSFER TIME value is greater than 100 but less than 149, the timing is
outside the acceptable range but the number greater than 100 indicates that cells are
definitely being detected. The TRANSFER TIME is controlled by the height of the
flow cell relative to the level of the light beam. Since the light beam is at a fixed
height with no vertical adjustment knob for the flow cell, this is a difficult
adjustment. The height of the flow cell is controlled by the spacers or shims placed
under the flow cell mounting block. Each shim moves the flow cell up a distance
that equates to a 30 microsecond drop in the TRANSFER TIME value. A new flow
cell is shipped with matching shims that should set it to the proper height. As a
result, it is critical that these shims are always replaced anytime the flow cell is
removed and replaced.
8. Verify the RESISTIVE CHANNEL value is between 45 and 55.
r If the value on the screen is outside this acceptable range or if you desire a value
closer to the target value (50), go to the Resistive Channel Adjustment heading.
9. Verify the ABSORBANCE CHANNEL value is greater than 170.
r If the value on the screen is acceptable, go to the Final Verification heading at the
end of this section.
r If the value on the screen is less than 170 or if you desire a higher value, go to the
Absorbance Channel Adjustment heading.
4.4-2 PN 4237616B
DIFF Lamp Voltage Adjustment
Note: If you are only interested in the DIFF LAMP voltage, it it not necessary to aspirate latex.
However, if you change the lamp voltage, the Transfer Time, Resistive Channel, and
Absorbance Channel values must be checked using latex particles.
Preparation
1. Remove the left side panel and the top cover from the instrument. For details, see
Heading 4.2. Set the left side panel and top cover aside.
2. Locate potentiometer R11 on the optical bench assembly (Figures 4.4-2 and 4.4-3). This
is the potentiometer you will use to adjust the DIFF lamp voltage.
Figure 4.4-2 Potentiometer R11 - Location on the Optical Bench Assembly
Figure 4.4-3 Potentiometer R11 Location - Top View from the Front of the Instrument
R11
7616032A
Adjustment
Note: If the Diff Adjustment screen is currently displayed, press ESC to return to the
Measurement Menu then select 5. DIFF ADJUSTMENT.
PN 4237616B 4.4-3

particles to the probe and press the aspirate switch to initiate aspiration and the routine
5. When the Diff Adjustment screen appears, monitor the DIFF LAMP value on the screen.
6.00 Vdc is the target value. The acceptable range is 5.50 to 6.50 Vdc.
6. If a change is desired, adjust potentiometer R11 (Figure 4.4-3) to produce a readout of
6.00 Vdc.
Note: Measurements are continuously displayed for 27 seconds. The readout updates
every 700 microseconds. Do not continue to make adjustments after the three audible
beeps.
7. If more time is needed to make the adjustment,
a. When the CYCLE IN PROGRESS timing bar disappears, press ESC to return to the
Measurement menu.
b. At the Measurement menu, select 5. DIFF ADJUSTMENT.
c. When the DIFF ADJUSTMENT prompt appears, press ENTER to continue.
d. Mix the RBC/PLT latex vigorously. Use a vortex, if available.
e. When the PLEASE SAMPLE LATEX prompt appears, present the vial of well-mixed
latex particles to the probe and press the aspirate switch to initiate aspiration and
the routine.
f. When the Diff Adjustment screen appears, the monitoring and adjustment process
can be continued for another 27 seconds until the three beeps sound.
Interim Verification
When the DIFF LAMP value is within the acceptable range of 5.50 to 6.50 Vdc, return to the
Flow Cell Checks heading to verify this new setting and make sure all the other values are
acceptable.
4.4-4 PN 4237616B
Resistive Channel Adjustment
Preparation
1. If you have not already done so, remove the left side panel from the instrument. For
details, see Heading 4.2. Set the panel aside.
2. Locate potentiometer R136 on the Main card. See Figure 4.4-4.
Figure 4.4-4 Main Card Flow Cell Adjustments
R148 R136
7616007A
PN 4237616B 4.4-5
Adjustment
3. Remix the RBC/PLT latex vigorously. Use a vortex, if available.

4. Present the vial of well-mixed latex particles to the probe and press the aspirate switch to
initiate aspiration and the adjustment routine.
5. When the Diff Adjustment screen appears, monitor the RESISTIVE CHANNEL value on
the screen. 50 is the target value. The acceptable range is 45 to 55.
Note: If the values are fluctuating, bubbles may be entering the flow cell. Purge the flow
cell as follows:
From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEM tt 2. RINSE tt
6. If a change is desired, adjust potentiometer R136 until the channel is set at 50.
beeps.
Measurement menu.
the routine.
When the RESISTIVE CHANNEL value is within the acceptable range of 45 to 55, return to
the Flow Cell Checks heading to verify this new setting and make sure all the other values are
acceptable.
4.4-6 PN 4237616B
Absorbance Channel Adjustment
During this adjustment, the flow cell is repositioned so that the focal point of the light beam
(which is fixed) is properly positioned inside the flow cell. The goal is to maximize the
absorbance using a two-positional adjustment.
Preparation
1. If you have not already done so, remove the left side panel and the top cover from the
instrument. For details, see Heading 4.2. Set the left side panel and top cover aside.
2. At the optical bench assembly, locate the front adjustment knob (Figure 4.4-5) and the
side adjustment screw (Figure 4.4-6).
r The Absorbance Channel adjustment is made using the front knob (Figure 4.4-5) or
side screw (Figure 4.4-6) to move the flow cell.
r An adjustment to move the flow cell along the Y-axis (front or back) is made using
the front knob.
r An adjustment to move the flow cell along the X-axis (right or left) is made using
the side screw.
Figure 4.4-5 Front Adjustment Knob - Optical Bench Figure 4.4-6 Side Adjustment Screw - Optical Bench
Adjustment
3. Remix the RBC/PLT latex vigorously. Use a vortex, if available.

4. Present the vial of well-mixed latex particles to the probe and press the aspirate switch to
initiate aspiration and the adjustment routine.
PN 4237616B 4.4-7
5. When the Diff Adjustment screen appears, verify the ABSORBANCE CHANNEL value is
greater than 170.
6. If a change is desired, use the front knob (Figure 4.4-5) and the side screw (Figure 4.4-6)
to adjust the channel higher than 170.
beeps. If you have difficulties adjusting the absorbance high enough and the DIFF LAMP
voltage value is acceptable, use potentiometer R148 on the Main card (Figure 4.4-4) to
increase the gain. Final adjustment of potentiometer R148 is done during verification
with fresh, normal whole-blood specimens.
Measurement menu.
the routine.
When the ABSORBANCE CHANNEL value is greater than 170, return to the Flow Cell Checks
heading to verify this new setting and make sure all the other values are acceptable.
4.4-8 PN 4237616B
Final Verification
When all adjustments are complete,
1. If the optical bench cover was removed, replace the cover and secure it with the four
screws removed earlier.
2. Make sure the right side door is closed.
3. Cycle several fresh normal whole-blood specimens (five different specimens, if possible)
and verify the DiffPlot looks acceptable. It is particularly important that the lymphocyte
and neutrophil population positions (Figure 4.4-7) meet the following criteria:
r The lymphocyte population must fall between the dotted lines representing normal
lymphocytes (area labeled Lymph). Very few, if any, cells should be located in the
small lymphocyte area (unlabeled area under the lower dotted line) or in the
atypical lymphocyte area (area labeled ATL above the upper dotted line). See
Figure 4.4-7.
Move the lymphocyte population up or down by adjusting potentiometer R136 as
needed (Figure 4.4-4). Base this resistive channel adjustment on several bloods, just
in case the population of a specific blood is not as normal as you think.
r The vertical line separating the lymphocyte (area labeled Lymph) and neutrophil
(Neut) populations must bisect those two populations. See Figure 4.4-7.
If needed, use potentiometer R148 (Figure 4.4-4) to adjust the absorbance channel
until the two populations are distinct and separated by the line.
4. If it was necessary to adjust either the resistive channel or the absorbance channel,
recheck the diff adjustments as follows:
a. From the Service menu, select 2. MEASUREMENT tt 5. DIFF ADJUSTMENT.
b. When the DIFF ADJUSTMENT prompt appears, press ENTER to continue.
PN 4237616B 4.4-9
IMPORTANT Risk of misleading results. The RBC/PLT latex particles tend to clump as they settle out
of solution. Clumped latex particles will affect adjustment results. Mix the RBC/PLT latex vigorously
before use. A vortex may be used. Remix the latex thoroughly before each sampling.
c. Mix the RBC/PLT latex vigorously. Use a vortex, if available.

d. When the PLEASE SAMPLE LATEX prompt appears, present the vial of well-mixed
the routine.
e. Verify the channel results appearing on the Diff Adjustment screen are acceptable:
r RESISTIVE CHANNEL value is between 45 and 55.
r ABSORBANCE CHANNEL value is greater than 170.
Note: If any value falls outside the acceptable range, the whole-blood specimens
may not be normal or there may be an instrument problem affecting the DiffPlot.
Timing, dilutions, noise, bubbles, or reagent can affect performance. Troubleshoot
as needed.
5. When system performance is acceptable,
a. Carefully close the Main card door. Turn the two captive knobs clockwise to secure
the door.
b. Replace the top cover if it was removed. See Heading 4.2 as needed.
ATTENTION: When replacing the left side panel with the instrument powered on, avoid
accidently turning the instrument off again by carefully positioning the opening for the
power on/off rocker switch over the switch as you position the panel on the instrument
frame.
c. Replace the left side panel and install the four hex screws that secure it to the
instrument frame.
6. Perform a system verification. See Heading 5.3, SYSTEM VERIFICATION
PROCEDURES.
4.4-10 PN 4237616B
BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT 4
4.5 BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT
Purpose
Use this procedure to properly position the baths assembly anytime the assembly is either
moved or replaced. Avoid any unnecessary contact with the sample probe. The probe is sharp
and may contain biohazardous materials.
B Feeler gauges, optional
B Flashlight, optional
Preparation
2. From the Main Menu, select 4. DIAGNOSTICS tt 4. HARDWARE SYSTEMS tt
4. TRAVERSE SERVICE POSITION.
3. When the TRAVERSE SERVICE POSITION prompt appears, press ENTER to retract the
probe and move the housing over the bath assembly.
5. Open the pneumatic access door (right side of the instrument).
Alignment Check
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the baths
assembly, you may become contaminated. To prevent possible biological contamination, you must wear
appropriate safety glasses, a lab coat, and gloves when servicing the baths assembly.
1. Using the horizontal traverse belt, manually position the sample probe housing over the
inside rim of the rinse bath.
PN 4237616B 4.5-1
BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT
2. Gently push down on the top of the sample probe until the tip of the probe rests on the
inside rim of the rinse bath (Figure 4.5-1 and Figure 4.5-2).
Figure 4.5-1 Sample Probe Position at the Rinse Bath Figure 4.5-2 Close-up of Probe Position
CAUTION Risk of damage to the sample probe. If power is restored to the instrument and the baths
assembly is mounted too high, the sample probe may become bent when it hits the bath edges as it moves
from bath to bath. Do not restore instrument power until the baths assembly is aligned.
3. Verify the tip of the sample probe evenly clears the top of each bath:
ATTENTION: If you have removed and replaced the baths assembly, be careful that you do
not bend the probe as you move it towards the WBC/BASO bath.
a. Without lifting the probe, gently push the probe housing towards the inside edge of
the last bath, the WBC/BASO bath.
b. Note the distance between the tip of the probe and the top of each bath as you move
towards the WBC/BASO bath (Figures 4.5-3 and 4.5-4).
Figure 4.5-3 Sample Probe at the WBC/BASO Bath Figure 4.5-4 Close-up of Probe at the WBC/BASO Bath
4. If the distance differs, do the Alignment Adjustment that follows.
4.5-2 PN 4237616B
BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT 4
Alignment Adjustment
1. Loosen, but do not remove, the three hex screws securing the baths assembly to the
support panel (Figure 4.5-5). Use a 3 mm Allen wrench.
Figure 4.5-5 Location of Screws Securing the Baths Support Panel
2. Manually push the sample probe housing to the outside edge of the rinse bath. Move the
baths assembly up or down as necessary until the tip rests gently on the outside rim of
the rinse bath.
3. While continuing to push the sample probe housing over the rinse bath to the inside
edge of the bath, adjust the baths assembly up or down as needed so that the sample
probe tip comes to rest gently on the inside rim of the rinse bath as shown in
(Figures 4.5-6 and 4.5-7).
.
Figure 4.5-6 Sample Probe Position at the Rinse Bath Figure 4.5-7 Close-up of Acceptable Probe Position
4. Gently tighten the center and left screws (See Figure 4.5-5).
5. Without lifting the probe, gently push the probe housing backwards towards the inside
edge of the last bath, the WBC/BASO bath. Reposition the baths assembly as needed to
clear the edge of each bath.
PN 4237616B 4.5-3
BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT
6. Verify the sample probe is positioned at the WBC/BASO bath as shown in Figure 4.5-8
and Figure 4.5-9.
Figure 4.5-8 Sample Probe Position, Right Side Figure 4.5-9 Close-up of Acceptable Probe Position
7. Gently tighten the right screw on the baths assembly (See Figure 4.5-5).
8. Make sure all three screws are tight so that the baths assembly is secure on the
instrument’s frame.
9. Carefully move the sample probe housing over the outer edge of the rinse bath. The
probe should equally clear all baths. If not, loosen the nearest screw and reposition the
baths assembly until the distance between the tip of the probe and the top of each bath is
the same.
Verification
1. Go back to the Alignment Check heading and perform this check to verify proper
alignment.
CAUTION It is mandatory to perform the probe adjustment procedure after this adjustment.
2. When the adjustment is correct

a. Reconnect the power cord.
b. Turn the instrument on. An automatic startup and background check are performed.
Note: When the startup routine and background check are done, the sample probe
will be back in its home position.
c. Go to Heading 4.6, SAMPLE PROBE CHECKS AND ADJUSTMENTS and perform
the probe adjustment procedure.
4.5-4 PN 4237616B
SAMPLE PROBE CHECKS AND ADJUSTMENTS 4
4.6 SAMPLE PROBE CHECKS AND ADJUSTMENTS
Purpose
IMPORTANT Check the baths assembly alignment before checking or adjusting the sample probe position.
See Heading 4.5, BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT.
Use this procedure to check the sample probe home position and the sample probe position
inside each bath. An adjustment procedure is provided after the check procedures. The check
procedures may be done at any time, but the adjustment procedures should be performed
only if the check fails.
From the Service menu, selecting 1. DILUTION provides the options needed to adjust the
traverse and sample probe (Figure 4.6-1.)
Figure 4.6-1 Dilution Screen r RUN: Lower prompt positions the probe
to determine if the tip of the sample
DILUTION 1/27/00 16:05 PM
probe is properly aligned with the DIFF
PROBE HOME 35 RUN bath at port 3.
TRAVERSE HOME 20
PROBE POS. 476 RUN r RUN CYCLE: Selection initiates a routine
TRAVERSE POS. 1132
that provides a way to determine if the
WBC / BASO TRAVERSE POS. 1060
FLOWCELL TRAVERSE POS. 737 tip of the sample probe is properly
RBC TRAVERSE POS. 734
RUN CYCLE aligned inside each of the baths. This
routine is similar to an actual sample
dilution.eps cycle.
r WBC/BASO TRAVERSE POS.: Probe
r PROBE HOME: Allow adjustment to get left/right position in the WBC/BASO
the best probe extremity cleaning. bath relative to the DIL1/HGB bath
position. Adjusts distance from
r TRAVERSE HOME: Default value is 20 and
DIL1/HGB bath to WBC/BASO bath.
does not need to be changed.
r FLOWCELL TRAVERSE POS.: Probe
r PROBE POS.: Probe up/down
left/right position in the DIFF bath
adjustment.
relative to the WBC/BASO bath. Adjusts
r TRAVERSE POS.: Probe left/right distance from WBC/BASO bath to DIFF
position based on Traverse Home. bath.
r RUN: Upper prompt positions the probe r RBC TRAVERSE POS.: Probe left/right
to determine if the tip of the sample position in the RBC bath relative to the
probe is properly positioned 9.4 mm DIFF bath. Adjusts distance from DIFF
from its guide. bath to RBC bath.
B Feeler adjustment gauge, 9.4 mm, or a sturdy piece of paper cut exactly 9.4 mm
(0.37 in.) wide and approximately 76.2 mm (3.0 in.) long
B Plastic transfer pipet
B Flashlight, optional
B Jeweler’s loop (x5 power magnifier with 2-inch focal length), optional
PN 4237616B 4.6-1
SAMPLE PROBE CHECKS AND ADJUSTMENTS
Sample Probe Checks

Use this procedure to check the home position of the sample probe and the probe’s position
inside each bath.
Home Position Check

1. From the Service menu, select 1. DILUTION.
2. At the Dilution screen, select the upper RUN option and press ENTER.
3. Open the right side door
4. Verify the distance between the tip of the sample probe and the traverse is about 9.4 mm.
Use either the feeler gauge or the 9.4 mm side of the paper you may have prepared. See
Figures 4.6-2 and 4.6-3.
Figure 4.6-2 Acceptable Distance between the Sample Figure 4.6-3 Measuring the Distance between the
Probe Tip and the Traverse Sample Probe Tip and the Traverse
r If the distance is approximately 9.4 mm, press ESC then proceed to the heading
Inside Bath Position Check.
r If the distance is unacceptable, continue to the Home Position Adjustment heading
to perform the adjustment procedure.
4.6-2 PN 4237616B
Inside Bath Position Check
1. At the Dilution screen, select the RUN CYCLE option at the bottom of the screen.
2. Remove the tubing connected to port 1 of the DIL1/HGB bath, port 3 of the DIFF bath,
port 1 of the RBC bath and port 2 of the WBC/BASO bath. These ports are located on the
right side of each bath about 1cm (1/2 inch) from the top.
3. Clean the ports of any fluid that may affect your line of sight through the port. A plastic
transfer pipet may be used to clear the ports.
4. With the following considerations in mind, evaluate the position of the probe tip in
relation to the bath port.
r Consider using a jeweler’s loop (x5 power magnifier with 2-inch focal length) and a
flashlight. These tools greatly enhance the image.
r To make a proper judgement, your eyes must be correctly positioned.
r The correct position for your eyes is when the port looks like Figure 4.6-4. If the
circles are not centered (as in Figure 4.6-5), reposition your gaze until the circles are
centered (as in Figure 4.6-4).
Figure 4.6-4 Correct View through the Port Figure 4.6-5 Incorrect View through the Port
r As you gaze through the port, the tip of the sample probe should mimic the position
of the tip shown in Figure 4.6-6, in each bath. The first bath is the DIL1/HGB bath.
Press ENTER to advance to the WBC/BASO bath and then again for the DIFF bath
and the RBC bath. Check that the probe position is correct in each bath.
PN 4237616B 4.6-3
Figure 4.6-6 Properly Adjusted Sample Probe Tip
5. If the probe is properly adjusted for all baths, reconnect the tubing to the bath ports. If
the probe is out of adjustment, continue on to the heading Inside Bath Position
Adjustment and follow the instructions to properly adjust the position of the probe.
Sample Probe Adjustments

Use this procedure to adjust the home position of the sample probe and the probe’s position
inside each bath.
Home Position Adjustment
CAUTION Check the probe position using the RUN functions before making an adjustment.
1. Thoroughly clean the exterior of the probe before making any adjustments.
2. At the Dilution screen, select the upper RUN option and press ENTER.
3. Use the 9.4 mm side of the paper to recheck the distance between the tip of the sample
probe and the traverse to confirm the gap is incorrect. See Figures 4.6-2 and 4.6-3.
4. Press ESC to cancel the previous function.
5. At the Dilution screen,
a. Adjust the PROBE HOME value as needed.
r If probe tip is too low (gap >9.4 mm), increase the PROBE HOME value.
r If probe tip is too high (gap <9.4 mm), decrease the PROBE HOME value.
b. Select the upper RUN option and press ENTER to rerun the routine.
6. Use the feeler gauge or the 9.4 mm side of the paper to recheck the distance between the
tip of the sample probe and the traverse.
r If the gap is now correct, press ESC then proceed to the heading Inside Bath Position
Adjustment.
r If the gap is still incorrect, repeat steps 4 through 6 until the gap is approximately
9.4 mm.
4.6-4 PN 4237616B
Inside Bath Position Adjustment
IMPORTANT Do not perform this probe adjustment procedure unless it is absolutely necessary.
1. At the Dilution screen, highlight the RUN CYCLE option and press ENTER.
ATTENTION: As you complete this procedure, it is imperative that as you gaze through the port
the circles are centered. When the sample probe is properly adjusted, the tip of the probe
should mimic the view shown in Figure 4.6-7.
Figure 4.6-7 Ideal Probe Position
2. The first bath that the probe stops at is the DIL1/HGB bath. Evaluate the position of the
probe tip in relation to the bath port.
r Is it a horizontal positioning problem? Is the probe is too forward (Figure 4.6-8) or
too backward (Figure 4.6-9)?
Figure 4.6-8 Improper Probe Tip Position - Figure 4.6-9 Improper Probe Tip Position -
Too Forward Too Backward
Note: The TRAVERSE POS. value moves the sample probe to the left or right.
r Is it a vertical positioning problem? Is the probe too high (Figure 4.6-10) or too low
(Figure 4.6-11)?
PN 4237616B 4.6-5
Figure 4.6-10 Improper Probe Tip Position - Figure 4.6-11 Improper Probe Tip Position -
Too High Too Low
Note: The PROBE POS. value moves the sample probe up or down.
3. Determine if changes to the position are required. If they are, press ENTER several times to
advance through the cycle. At the Dilution screen, adjust the PROBE POS. or the
TRAVERSE POS. values as needed.
r The TRAVERSE POS. value moves the sample probe to the left or right (horizontal
positioning).
t If the probe is too forward (Figure 4.6-8), increase TRAVERSE POS. value.
t If the probe is too backward (Figure 4.6-9), decrease TRAVERSE POS. value.
r The PROBE POS. value moves the sample probe up or down (vertical positioning).
t If the probe is too high (Figure 4.6-10), increase the PROBE POS. value.
t If the probe is too low (Figure 4.6-11), decrease the PROBE POS. value.
4. Highlight and ENTER the RUN CYCLE function again.
5. If the probe position in the DIL1/HGB bath is acceptable, press the ENTER key once to
advance to the WBC/BASO bath.
6. Check the probe position in the WBC/BASO bath. At this point, there should be no
problem with height. If there is, the bath assembly is not level and proceed to the
heading BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT. When level,
start this procedure again at step 1.
r If the lateral probe position is not correct, adjust the WBC/BASO Traverse Position
value. You must press ENTER several times and advance through the cycle to make
any changes. Increase the number to move the probe farther back, or to the right,
decrease to move frontward of to the left. Note that it is rare to change from the
default value of 1060.
r Any changes to this value will almost certainly require changes to the flow cell
traverse position and the RBC traverse position, since these are relative moves from
the previous bath position.
8. Advance to the WBC/BASO bath, if not already there, and check the probe position in
each bath. If no changes are required, press ENTER to advance to the DIFF bath.
4.6-6 PN 4237616B
9. Check the position in the DIFF bath. The default value is 737 and is usually acceptable.
If changes are required, press ENTER several times to advance through the cycle. Since the
probe was moving back to front, increasing this number moves the probe towards the
front or to the left, decreasing the number moves the probe towards the back or to the
right. If there were no changes, go to step 11.
11. Advance to the DIFF bath, if not already there, checking the probe position in each bath.
If the positions are acceptable, and no further changes are made, press ENTER and
advance to the RBC bath.
12. Check the position in the RBC bath. The default value of 734 is usually good. If a change
is required, press ENTER several times to exit the cycle and adjust the RBC Traverse
Position value as required. Highlight and ENTER the RUN CYCLE function again. Check
the probe position in each bath.
WARNING Risk of contamination. If one or more tubings are left unattached to their respective bath ports
and you run a cycle, fluid will extrude out the ports onto the exterior of the baths, the counting heads, and
valves. It may result in the need for extensive cleanup inside the bath enclosure. To prevent possible
biological contamination, you must wear appropriate safety glasses, a lab coat, and gloves.
CAUTION Risk of component damage to electronic components. If one or more tubings are left unattached
to their respective bath ports and you run a cycle, fluid will extrude out the ports into the bath enclosure.
Excessive moisture may damage the Hgb circuitry and solenoid valves. Thoroughly wipe dry electronic
components as quickly as possible.
IMPORTANT Risk of misleading results. If one or more tubings are left unattached to their respective bath
ports and you run a cycle, fluid will extrude out the ports onto the exterior of the baths, the counting heads,
and valves. Moisture on these components affects parameter results. Thoroughly wipe components dry
before reporting patient results.
13. When no changes are required, replace each tubing back on its designated bath port.
PROCEDURES.
PN 4237616B 4.6-7
4.6-8 PN 4237616B
HGB BLANK ADJUSTMENT 4
4.7 HGB BLANK ADJUSTMENT
Purpose
Use this procedure to adjust the Hgb blank voltage. This adjustment must be made under
stabilized thermal conditions.
Preparation
IMPORTANT The Hgb blank voltage adjustment must be made under stabilized thermal conditions. Make
sure the right side door is closed before starting this adjustment. After completing a Startup, wait at least
5 minutes before performing this adjustment procedure.
1. Close the right side door to stabilize the temperature inside the bath enclosure.
2. Remove the left side panel to access the Main card. For details, see Heading 4.2.
3. Locate potentiometer R248 in the upper right quadrant of the Main card (Figure 4.7-1).
This is the adjustment potentiometer.
Figure 4.7-1 Main Card Hgb Blank Adjustment
R248
7616006A
PN 4237616B 4.7-1
HGB BLANK ADJUSTMENT
Adjustment
1. From the Service menu, select 2. MEASUREMENT tt 1. HGB BLANK ADJUSTMENT.
2. When the HGB BLANK ADJUSTMENT prompt appears, press ENTER to continue.
Note: Selecting this option initiates a routine that drains and rinses the DIL1/HGB bath
and continuously displays (for 20 seconds) the Hgb blank voltage used by the converter.
Three audible beeps signal the end of the 20 seconds.
3. While monitoring the Hgb Adjustment screen, adjust potentiometer R248 until the
voltage reads 4.7 Vdc ±0 Vdc.
Verification
1. Run a Startup and verify the Hgb blank result is acceptable.
2. Run a whole-blood sample to establish a new Hgb reference blank.
4.7-2 PN 4237616B
APERTURE CURRENT CHECK 4
4.8 APERTURE CURRENT CHECK
Purpose
Use this procedure to verify the voltages needed to generate aperture current are present and
at sufficient levels. These voltages are not adjustable.
B Digital voltmeter
B Old coax
Procedure
1. Open the left side panel to access the Main card. For details, see Heading 4.2.
2. On the Main card, disconnect the LMNE CIS (for DIFF analysis), GR RBC, and GB WBC
coaxes (Figure 4.8-1).
Figure 4.8-1 LMNE CIS, GR (RBC), and GB (WBC) Coax Locations
3. From the Service menu, select 2. MEASUREMENT tt 2. APERTURE CURRENT.

4. When the Aperture Current prompt appears, press ENTER.
CAUTION Do not damage connectors while measuring.
IMPORTANT Use an old coax that you have previously cut to check these voltages with the voltmeter. A
flow cell coaxial cable with the T- connector removed is ideal.
5. When the CHECK APERTURE CURRENT prompt appears, use a DVM to verify the
voltages at J22, J23 and J24 (Figure 4.8-1) are approximately 60 Vdc. These voltages are
not adjustable.
6. Press ENTER to exit the routine.
7. Reconnect the LMNE CIS, GR RBC and GB WBC coaxes (Figure 4.8-1).
PN 4237616B 4.8-1
APERTURE CURRENT CHECK
accidently turning the instrument off by carefully positioning the opening for the power
on/off rocker switch over the switch as you position the panel on the instrument frame.
8. Replace the left side panel and install the four hex screws that secure it to the instrument
frame.
4.8-2 PN 4237616B
RBC/PLT GAIN ADJUSTMENT 4
4.9 RBC/PLT GAIN ADJUSTMENT
Purpose
Use this procedure to adjust the RBC and Plt gains. A special cycle automatically makes a
1:220 dilution of the RBC/PLT latex (10 µL of latex to 2.2 mL of diluent). A special count
program carries out the calculations of the mean volume in the predefined zones and displays
them every 700 ms. The duration of the measurement cycle is 21 seconds.
B Allen wrench, 3.0 mm
Procedure
Preparation
2. Locate potentiometers R133 for the RBC adjustment and R135 for the PLT adjustment in
the upper right quadrant of the Main card (Figure 4.9-1).
Figure 4.9-1 Main Card RBC/PLT Gain Adjustments
LMNE LMNE GR GB
OD CIS RBC WBC
R135 R133
LMNE LMNE GR GB
OD CIS RBC WBC
7616008B
PN 4237616B 4.9-1
RBC/PLT GAIN ADJUSTMENT
3. From the Service menu, select 2. MEASUREMENT tt 3. RBC / PLT GAIN.

4. When the RBC/PLT GAIN prompt appears, press ENTER to continue.
solution. Clumped latex particles will affect adjustment results. Mix RBC/PLT latex vigorously before use. A
vortex may be used. Remix the latex thoroughly before each sampling.

Adjustments
1. While monitoring the RBC bar graph on the RBC/PLT Gain screen, adjust potentiometer
R133 to read 78 (target value for RBC).
2. While monitoring the PLT bar graph on the RBC/PLT Gain screen, adjust potentiometer
R135 to read 112 (target value for PLT).
4.9-2 PN 4237616B
WBC/BASO GAIN ADJUSTMENT 4
4.10 WBC/BASO GAIN ADJUSTMENT
Purpose
Use this procedure to adjust the WBC and BASO gains. A special cycle automatically makes a
1:733 dilution of the RBC/PLT latex (3 µL of latex to 2.2 mL of diluent). A special count
program carries out the calculations of the mean volume in the predefined zones and displays
them every 700 ms. The duration of the measurement cycle is 21 seconds.
Procedure
Preparation
2. Locate potentiometer R134 in the upper right quadrant of the Main card (Figure 4.10-1).
This is the adjustment potentiometer.
Figure 4.10-1 Main Card WBC/BASO Gain Adjustment
LMNE LMNE GR GB
OD CIS RBC WBC
R134
LMNE LMNE GR GB
OD CIS RBC WBC
7616009B
PN 4237616B 4.10-1
WBC/BASO GAIN ADJUSTMENT
3. From the Service menu, select 2. MEASUREMENT tt 4. WBC / BASO GAIN.

4. When the WBC/BASO GAIN prompt appears, Press ENTER to continue.
solution. Clumped latex particles will affect adjustment results. Mix RBC/PLT latex vigorously before use. A
vortex may be used. Remix the latex thoroughly before each sampling.

Adjustment
While monitoring the WBC/BASO bar graph on the WBC/BASO Gain screen, adjust
potentiometer R134 to read 102 (target value).
4.10-2 PN 4237616B
DRAIN SENSOR ADJUSTMENT 4
4.11 DRAIN SENSOR ADJUSTMENT
Purpose
Use this procedure to adjust the drain sensor. As preparation for this adjustment, this routine
automatically empties and fills the drain cell.
B Digital voltmeter (DVM)
Preparation
1. Remove the four hex screws securing the left side panel to the instrument frame. Set the
door aside.
2. Locate potentiometer R287 and test point TP52 in the lower left quadrant of the Main
card (Figure 4.11-1).
Figure 4.11-1 Main Card Drain Sensor Adjustment
LMNE LMNE GR GB
OD CIS RBC WBC
7616010B
R287 TP52
PN 4237616B 4.11-1
DRAIN SENSOR ADJUSTMENT
Adjustment
1. From the Service menu, select 5. SENSOR CHECK tt 1. DRAINING. The Draining prompt
appears on the screen.
2. Press ENTER to continue. The CYCLE IN PROGRESS bar graph appears and advances to
approximately 48% while draining the sensor.
3. When the ADJUST SENSOR VOLTAGE prompt appears,
a. Use a DVM to verify the voltage at TP52 is 4.5 ±0.3.
b. Adjust the voltage at potentiometer R287 as needed.
c. Press ENTER to continue. The CYCLE IN PROGRESS bar graph advances to
approximately 53% as the sensor is filled with diluent.
4. When the CHECK SENSOR VOLTAGE prompt appears,
a. Make sure the voltage at TP52 is <1.0 Vdc.
b. Press ENTER to continue. The CYCLE IN PROGRESS bar graph advances to 100% as
the routine is completed.
Wrap Up
1. Press ESC as many times as necessary to return to the Main Menu.
frame.
4.11-2 PN 4237616B
TRANSFER SENSOR ADJUSTMENT 4
4.12 TRANSFER SENSOR ADJUSTMENT
Purpose
Use this procedure to adjust the transfer sensor. As preparation for this adjustment, this
routine automatically empties then fills the drain sensor. This sensor controls the transfer of
the diluted sample from the Diff bath to the flow cell.
Preparation
1. Remove the four hex screws securing the left side panel to the instrument frame. Set the
door aside.
2. Locate potentiometer R286 and test point TP48 in the lower left quadrant of the Main
card (Figure 4.12-1).
Figure 4.12-1 Main Card Transfer Sensor Adjustment
LMNE LMNE GR GB
OD CIS RBC WBC
7616003B
R286 TP48
PN 4237616B 4.12-1
TRANSFER SENSOR ADJUSTMENT
Adjustment
1. From the Service menu, select 5. SENSOR CHECK tt 2. DIFF TRANSFER. The Diff Transfer
prompt appears on the screen.
2. Press ENTER to continue. The CYCLE IN PROGRESS bar graph appears and advances to
approximately 48% while draining the sensor.
3. When the ADJUST SENSOR VOLTAGE prompt appears,
a. Use a DVM to verify the voltage at TP48 is 4.5 ±0.3.
b. Adjust the voltage at potentiometer R286 as needed.
c. Press ENTER to continue. The CYCLE IN PROGRESS bar graph advances to
approximately 53% as the sensor is filled with diluent.
4. When the CHECK SENSOR VOLTAGE prompt appears,
a. Make sure the voltage at TP48 is <1.0 Vdc.
b. Press ENTER to continue. The CYCLE IN PROGRESS bar graph advances to 100% as
the routine is completed.
Wrap Up
frame.
4.12-2 PN 4237616B
MOTOR CURRENT ADJUSTMENT 4
4.13 MOTOR CURRENT ADJUSTMENT
Purpose
Use this procedure to check or adjust the voltages for the motors that supply power for the
various syringe assemblies and the sampling carriage on the Traverse module.
Procedure
1. Open the left side door to access the Main card.
2. Check and adjust, when necessary, the voltages in Table 4.13-1 using the designated
potentiometers and test points located in the upper left quadrant of the Main card
(Figure 4.13-1).
Table 4.13-1 Motor Voltage Limits
Motors Test Point Voltage Potentiometer

Drain syringe TP5 4 V ±0.05 V R149
Count syringe TP6 4 V ±0.05 V R150
Reagent syringes TP7 4 V ±0.05 V R151
Optical bench injector syringe TP8 3 V ±0.05 V R152
Horizontal traverse TP10 3 V ±0.05 V R154
Sample syringe TP11 2 V ± 0.05 V R155
Vertical traverse TP12 4.5 V ±0.05 V R156
PN 4237616B 4.13-1
MOTOR CURRENT ADJUSTMENT
Figure 4.13-1 Main Card Motor Current Adjustments
LMNE LMNE GR GB
TP5/R149
OD CIS RBC WBC
waste syringe
T P 5
TP6/R150
T P 6
count syringe
T P 7 TP7/R151
reagent syringes
T P 8 TP8/R152
optical bench
injector syringe
T P 9
T P 1 0 TP10/R154
horizontal
traverse
T P 1 1
TP11/R155
T P 1 2
sample syringe
TP12/R156
vertical traverse
7616004B
4.13-2 PN 4237616B
THRESHOLD ADJUSTMENTS 4
4.14 THRESHOLD ADJUSTMENTS
Purpose
Use this procedure to check or adjust the threshold voltages as needed.
B Digital voltmeter
Procedure
1. Open the left side door to access the Main card.
2. Check and adjust, when necessary, the voltages in Table 4.14-1 using the designated
potentiometers and test points in the upper area of the Main card (Figure 4.14-1).
Table 4.14-1 Threshold Voltage Limits
Threshold Test Point Voltage Potentiometer

BASO TP14 300 mV ±5 R157
RBC TP13 300 mV ±5 R158
PLT TP2 300 mV ±5 R159
LMNE CIS (for DIFF) TP3 650 mV ±5 R160
LMNE OD (for DIFF) TP4 350 mV ±5 R161
PN 4237616B 4.14-1
THRESHOLD ADJUSTMENTS
Figure 4.14-1 Main Card Threshold Adjustments
LMNE LMNE GR GB
OD CIS RBC WBC
LMNE LMNE GR GB
OD CIS RBC WBC
7616005B
LMNE (DIFF) OD LMNE (DIFF) CIS PLT RBC BASO
(TP4/R161) (TP3/R160) (TP/R159) (TP13/R158) (TP14/R157)
4.14-2 PN 4237616B
REAGENT TEMPERATURE CHECK AND ADJUSTMENT 4
4.15 REAGENT TEMPERATURE CHECK AND ADJUSTMENT
Purpose
Use this procedure to check proper operation of the heating coil and associated system
components. This check and adjustment must be made under stabilized thermal conditions.
B Thermometer probe
B Digital voltmeter
Reagent Temperature Check

2. Place the thermometer probe into the upper portion of the fluid in the DIFF bath. Avoid
touching the sides and bath electrodes (Figure 4.15-1).
If using a wire bead, bend and insert the bead through the top of the unused fitting of the
bath.
Figure 4.15-1 Thermometer Probe inside the DIFF Bath
3. Insert the thermometer probe leads in the DVM.
IMPORTANT The reagent temperature check must be done under stabilized thermal conditions. Make sure
the right side door is closed before starting this check. After completing a startup, wait at least 5 minutes
before performing this temperature check.
PN 4237616B 4.15-1
REAGENT TEMPERATURE CHECK AND ADJUSTMENT
4. Carefully close the right side door to ensure the thermometer probe remains submerged
in the reagent.
5. Open the left side door.
6. In the lower left quadrant of the Main card, locate the LED near connector J31
(Figure 4.15-2) and verify the LED is flashing (rapidly).
Note: This LED indicates heating status:
r When the LED is off, the heating coil is off. When the LED is on, the heating coil is
on.
r A slow blinking LED indicates the heating coil is in the process of increasing or
decreasing the temperature.
r When the LED is flashing rapidly, the heating coil is maintaining the target
temperature.
Figure 4.15-2 Main Card Heating Status LED Location
7. From the Service menu, select 3. HEATING SYSTEMS tt 1. HEATING COIL tt 2. REFERENCE.
8. At the Reference screen,
a. Set the RUN CYCLE NUMBER to 5.
IMPORTANT Make sure the thermometer probe is submerged in the liquid before starting this routine.
b. Select RUN then press ENTER.

9. Each time the instrument beeps, compare the REFERENCE TEMPERATURE with the
RUNNING TEMPERATURE LIMITS displayed on the screen. Monitor the temperature
reading through at least 10 beeps.
r If the temperature remains within the limits all 10 times, the heating system is
working properly. Remove the temperature probe and close the right and left side
doors.
r If the temperature falls outside the displayed limits, perform the Reagent
Temperature Adjustment procedure.
4.15-2 PN 4237616B
REAGENT TEMPERATURE CHECK AND ADJUSTMENT 4
Reagent Temperature Adjustment
ATTENTION: Always perform the Reagent Temperature Check before making this adjustment.
Preparation
1. Perform a Reagent Temperature Check if you have not already so.
IMPORTANT The reagent temperature adjustment must be done under stabilized thermal conditions. Make
sure the right side door is closed before starting this adjustment. After completing a startup, wait at least 5
minutes before performing this temperature adjustment.
2. From the Service Menu, select 3. HEATING SYSTEMS tt 1. HEATING COIL tt 1.ADJUSTMENT.
The prompt TEMP. ADJ VALUE XXXX appears.
3. In the lower left quadrant of the Main card, locate connector J31 and find the temperture
value written on the cable (Figure 4.15-3).
Note: Make sure you read the correct value, the sticker may be oriented up or down.
Figure 4.15-3 Location of the Label Containing the Temperature Value for the Heater Assembly
7616034A
4. Verify the temperature value on the screen agrees with the temperature value on the
cable.
r If the temperature values agree, press ESC to return to Heating Coil menu then
proceed to the Adjustment procedure that follows.
r If the temperature values do not agree, change the screen value to match the value
written on the cable, press ESC to return to the Heating Coil menu, then continue on
to the Adjustment procedure that follows.
PN 4237616B 4.15-3
REAGENT TEMPERATURE CHECK AND ADJUSTMENT
Adjustment
1. From the Heating Coil menu, select 2. REFERENCE.
IMPORTANT Make sure the thermometer probe is submerged in the liquid before starting this routine.

working properly. Remove the temperature probe and close the left side door.
r If the temperature falls outside the displayed limits, go to step 4.
4. Adjust the TEMPERATURE REFERENCE value as needed.
Note: When the temperature reference is increased, the reagent preheating temperature
increases; when decreased, the reagent preheating temperature decreases. As the
requested temperature change occurs, the heating status LED is blinking slowly.
5. In the lower left quadrant of the Main card, monitor the heating status LED
(Figure 4.15-2).
6. When the heating status LED is flashing rapidly (indicating the target temperature is
achieved), repeat steps 2 through 5 until you obtain an acceptable temperature. If it is
not possible to properly adjust the temperature, change the heating coil using the
instructions under Heading 4.19, HEATER ASSEMBLY REPLACEMENT.
4.15-4 PN 4237616B
BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT 4
4.16 BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT
Purpose
Use this procedure to check proper operation of the bath enclosure fan and associated system
components. These checks and adjustments must be made under stabilized thermal
conditions.
B Thermometer probe
B Digital voltmeter
Bath Enclosure Temperature Check

2. Place the thermometer probe close to the temperature sensor (Figure 4.16-1).
Figure 4.16-1 Temperature Sensor Location - View with the Right Side Door Open
3. Insert the thermometer probe leads in the DVM.
IMPORTANT The bath enclosure temperature check must be done under stabilized thermal conditions.
Make sure the right side door is closed before starting this check. After completing a startup, wait at least 5
minutes before performing this temperature check.
4. Carefully close the right side door to ensure the thermometer probe remains close to the
temperature sensor.
5. From the Service menu, select 3. HEATING SYSTEMS tt 2. BATH ENCLOSURE tt 2. REFERENCE.
IMPORTANT Make sure the thermometer probe is close to the temperature sensor before starting this
routine.
PN 4237616B 4.16-1
BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT
working properly. Remove the temperature probe.
r If the temperature falls outside the displayed limits, perform the Bath Enclosure
Temperature Adjustment procedure.
Bath Enclosure Temperature Adjustment

ATTENTION: Always perform the Bath Enclosure Temperature Check before making this
adjustment.
Preparation
1. Perform a Bath Enclosure Temperature Check if you have not already so.
IMPORTANT The bath enclosure temperature adjustment must be done under stabilized thermal
conditions. Make sure the right side door is closed before starting this adjustment. After completing a
startup, wait at least 5 minutes before performing this temperature adjustment.
2. From the Service menu, select 3. HEATING SYSTEMS tt 2. BATH ENCLOSURE tt

1.ADJUSTMENT. The prompt TEMP. ADJ VALUE XXXX appears.
3. In the lower left quadrant of the Main card, locate connector J33 and find the
temperature value written on the cable (Figure 4.16-2).
Note: Make sure you read the correct value, the sticker may be oriented up or down.
Figure 4.16-2 Location of the Label Containing the Temperature Value for the Temperature Sensor
7616034A
4.16-2 PN 4237616B
BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT 4
4. Verify the temperature value on the screen agrees with the temperature value on the
cable.
r If the temperature values agree, press ESC to return to Bath Enclosure menu then
proceed to the Adjustment procedure that follows.
r If the temperature values do not agree, change the screen value to match the value
written on the cable, press ESC to return to the Bath Enclosure menu, then continue
on to the Adjustment procedure that follows.
Adjustment
1. From the Bath Enclosure menu, select 2. REFERENCE.
IMPORTANT Make sure the thermometer probe is close to the temperature sensor before starting this
routine.

r If the temperature remains within the limits all 10 times, the bath enclosure
temperature monitoring system is working properly. Remove the temperature probe
and close the right side door.
r If the temperature falls outside the displayed limits, go to step 4.
4. Adjust the REFERENCE TEMPERATURE value as needed.
r This adjustment controls the operation of a bath enclosure fan and heater.
r If the reference temperature is increased, the fan and a heater mechanism inside the
fan is turned on until the ambient bath enclosure temperature reaches the desired
temperature.
r If the reference temperature is decreased, the fan is turned on to pull atmospheric
air into the bath enclosure until the ambient temperature inside the enclosure
reaches the desired temperature.
r If it is not possible to properly adjust the temperature, check fan operation.
PN 4237616B 4.16-3
BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT
4.16-4 PN 4237616B
VACUUM CHECKS AND ADJUSTMENTS 4
4.17 VACUUM CHECKS AND ADJUSTMENTS
Purpose
Use this procedure to check waste or count syringe vacuum and to adjust the count syringe
vacuum, as needed. Since altitude affects vacuum, these vacuum checks should be performed
anytime an instrument is being installed in a location that is either above or below sea level.
B External digital pressure/vacuum gauge; hereafter, referred to as a vacuum meter.
Waste Syringe Vacuum Check

2. Remove the tubing attached to the side of the waste syringe and replace it with a tubing
attached to the vacuum meter, as shown in Figure 4.17-1.
Figure 4.17-1 Attach the Vacuum Meter to the Waste Syringe
IMPORTANT Risk of misleading results. The syringe creates a finite vacuum. It does not have continuous
flow and evacuation like a pump. If the tubing attached to the vacuum meter is too large, the volume of the
tubing adds to the volume in the syringe and may produce falsely low readings.
3. From the Service menu, select 6. VACUUM CHECK tt 2. DRAINING.

4. When the CHECK VACUUM prompt appears on the screen, check the vacuum reading
on the vacuum meter. The vacuum must be approximately 260 mb (7.7-inches Hg) and
must be stable.
Note: No vacuum adjustment is available for the waste syringe.
PN 4237616B 4.17-1
VACUUM CHECKS AND ADJUSTMENTS
5. Disconnect the vacuum meter and reattach the tubing on the waste syringe.
Count Syringe Vacuum Check and Adjustment
Count Syringe Vacuum Check

1. Remove the left side panel. For details, see Heading 4.2.
CAUTION A flat cable is connected to the rear of the Main card. Be careful when you open the door
(where the Main card is attached) that you do not disconnect or damage this cable.
2. In the left side compartment,

a. Turn the two captive knobs counterclockwise to release the Main card door. These
knobs are located to the right of the Main card.
b. Open the Main card door and carefully anchor the door behind the white plastic
catch to keep it open. The optical bench is exposed.
IMPORTANT Risk of misleading results. The syringe creates a finite vacuum. It does not have continuous
flow and evacuation like a pump. If the tubing attached to the vacuum meter is too large, the volume of the
tubing adds to the volume in the syringe and may produce falsely low readings.
3. At the count syringe, disconnect the side tubing nearest the bottom of the syringe and
replace it with a tubing attached to the vacuum meter, as shown in Figure 4.17-2.
Figure 4.17-2 Attach the Vacuum Meter to the Count Syringe
4.17-2 PN 4237616B
VACUUM CHECKS AND ADJUSTMENTS 4
4. From the Service menu, select 6. VACUUM CHECK tt 1. COUNTING.
5. When VACUUM XXX prompt appears on the screen, the down arrow key select the RUN
prompt.
6. When the CHECK VACUUM (XXX MB) prompt appears, check the vacuum reading on
the vacuum meter. The vacuum must be approximately 220 mb (6.5-inches Hg) and
stable.
r If the vacuum level is correct and stable, go to step 7.
r If the vacuum level is too low or unstable, press ESC to erase the CHECK VACUUM
(XXX MB) prompt then go to the Count Syringe Vacuum Adjustment heading to
make the appropriate adjustment.
7. Disconnect the vacuum meter and reattach the tubing on the count syringe.
8. Close the Main card door. Turn the two captive knobs clockwise to secure the door.
frame.
11. Continue operation.
Count Syringe Vacuum Adjustment

1. At the Counting screen, use the up arrow to move the cursor to the VACUUM XXX
prompt then change the step value for vacuum.
r If the vacuum is too low, increase the step value.
r If the vacuum is too high, decrease the step value.
2. Select the RUN prompt.
3. When the prompt CHECK VACUUM (XXX MB) appears, check the vacuum reading on
the vacuum meter. The vacuum must be approximately 220 mb (6.5-inches Hg) and
stable.
r If the vacuum level is correct and stable, go to step 4.
r If the vacuum level is still unacceptable, press ESC and repeat steps 1 through 3 until
the vacuum is stable at approximately 220 mb (6.5-inches Hg).
4. Disconnect the vacuum meter and reattach the tubing on the count syringe.
5. Close the Main card door. Turn the two captive knobs clockwise to secure the door.
frame.
8. Continue operation.
PN 4237616B 4.17-3
VACUUM CHECKS AND ADJUSTMENTS
4.17-4 PN 4237616B
MIX BUBBLE ADJUSTMENT 4
4.18 MIX BUBBLE ADJUSTMENT
Purpose
Mixing bubbles are factory adjusted and normally do not require further adjustments;
however, if an adjustment is necessary follow this procedure.
Tools/Supplies Required
B None
Procedure
1. From the Service menu, select 4. MIXING.
2. At the Mixing screen, enter a new step value to increase or decrease the mixing, as
needed. Use the values in Table 4.18-1 as a guide.
r To increase bubbling, increase the number of steps.
r To decrease bubbling, decrease the number of steps.
Table 4.18-1 Mixing Bubble Limits
Mixing Bath Low Limit Normal High Limit

FIRST DILUTION 100 300 400
DIFF 100 300 400
WBC/BASO 100 300 400
HGB LYSE 300 400 500
3. Verify the final step values are within limits (Table 4.18-1).
PN 4237616B 4.18-1
MIX BUBBLE ADJUSTMENT
4.18-2 PN 4237616B
HEATER ASSEMBLY REPLACEMENT 4
4.19 HEATER ASSEMBLY REPLACEMENT
Purpose
Use this procedure to replace the heater assembly.
B Flat-blade screwdriver
B Torx keys
B Heater reagent coil assembly, PN - XDA625AS
Removal
1. To access the heater assembly:
a. Remove the front panel. Follow cover removal under Heading 4.2.
b. Pull the reagent syringes and the 5 diff syringe to free space behind (no need to
disconnect tubes).
ATTENTION: The syringe assemblies uses captive hex screws mounted inside rubber shock
mounts. It is recommended that you use only four turns to either loosen or tighten these
screws. Turning the hex screw too many counterclockwise rotations may separate the
screw from the rubber shock mount. If all Service Representatives consistently use four
turns to remove or install these hex screws, it is unlikely that a rubber shock mount will
separate from its hex screw and fall inside the instrument.
c. Locate the six hex screws (CHC M4x16) shown in Figure 4.19-1. These are captive
screws anchored inside rubber shock mounts.
Figure 4.19-1 Captive Hex Screw Locations - Reagent Syringes and 5diff Syringe
PN 4237616B 4.19-1
HEATER ASSEMBLY REPLACEMENT
d. Loosen each hex screw with four counterclockwise rotations.

Note: If a screw is not released in four rotations, the screw was overtightened the
last time the syringe assembly was serviced. From this point, make a single rotation
then check to see if the mount is free. Repeat this sequence of making a single
rotation and checking until the mount is free.
3. Disconnect the following tubings:
r At the WBC/BASO bath, disconnect the tubing attached to port 2.
r At the RBC bath, disconnect the tubing attached to port 1.
r At the DIFF bath, disconnect the tubing attached to ports 2 and 3.
r At the DIL1/HGB bath, disconnect the tubing attached to port 1.
4. Unscrew the two CHC M3x6 screws shown in Figure 4.19-2.
Figure 4.19-2 CHC M3 x 6 Screw Locations
4.19-2 PN 4237616B
5. The heater assembly is free. Lay the assembly on the instrument frame (Figure 4.19-3).
Figure 4.19-3 Heater Assembly
6. Move the heater assembly as close as possible to the opening in the instrument’s frame.
7. Using Figure 4.19-4 as a guide, disconnect the tubings attached to ports 1, 3, 5, 7, and 10
on the heater assembly.
Note: The ports are associated with the following components:
r The tubing disconnected from port 1 is from valve 22, port 2.
r The tubing disconnected from port 5 is from T4.
Figure 4.19-4 Heater Assembly - Tubing Port Locations
PN 4237616B 4.19-3
8. Locate connector P33 in the lower left quadrant of the Main card (Figure 4.19-5).
Figure 4.19-5 Main Card Heater Assembly Replacement
J33 7616015A
9. Remove the heater assembly cable attached to J33.
4.19-4 PN 4237616B
10. Pull the disconnected cable (and tubings) through the instrument and remove the heater
assembly (Figure 4.19-6).
Figure 4.19-6 Heater Assembly After Removal
11. Place the heater assembly on a stack of absorbent paper towels to drain it.
Installation
1. Place the new heater assembly next to the old heater assembly. Make sure they are
oriented in the same direction (cables on the same side, ports facing the same direction).
2. One by one, disconnect a tubing from the old heater and attach it in the same location on
the new heater. Use Figure 4.19-7 as a guide if needed.
Figure 4.19-7 Heater Assembly - Tubing Port Locations
Note: The tubings being removed from the ports on the old heater and attached to the
ports on new heater are associated with the following components:
r The tubing attached to port 2 will later be attached to the diluent bath, DIL1/HGB.
r The tubing attached to port 4 will later be attached to the DIFF bath, DIFF 3.
r The tubing attached to port 6 will later be attached to the DIFF bath, DIFF 2.
r The tubing attached to port 8 will later be attached to the RBC bath, RBC 1.
r The tubing attached to port 9 loops to port 11 on the heater assembly.
r The tubing attached to port 12 will later be attached to the WBC/BASO bath,
WBC/BASO 2.
PN 4237616B 4.19-5
3. Discard the old heater assembly.

4. Position the new heater assembly (with the old tubing attached) inside the instrument
frame (Figure 4.19-8).
Figure 4.19-8 Heater Assembly - Orientation Inside the Instrument
5. Using Figure 4.19-9 as a guide, one by one, locate each tubing removed from the old
heater assembly and attach the tubing to its designated port on the new heater assembly:
a. Locate the tubing attached to valve 11, port 2. Attach this tubing to port 10 of the
heater assembly.
b. Locate the tubing attached to valve 22, port 1. Attach this tubing to port 7 of the
heater assembly.
c. Locate the tubing attached to T4. Attach this tubing to port 5 of the heater assembly.
d. Locate the tubing attached to valve 8, port 2. Attach this tubing to port 3 of the
heater assembly.
Figure 4.19-9 Heater Assembly - Port Locations
4.19-6 PN 4237616B
e. Locate the tubing attached to valve 22, port 2. Attach this tubing to port 1 of the
heater assembly.
6. Remove the three nuts shown in Figure 4.19-10. Removing these nuts dismantles the
bath assembly from the instrument frame so that tubings attached to the new heater
assembly can be routed through the panel openings for attachment to the designated
bath.
Figure 4.19-10 Baths Assembly Support Panel Nut Locations
7. Lift and position the heater assembly so that each tubing disconnected during the
removal procedure can be routed through the support panel opening closest to the bath
where the tubing must be attached (Figure 4.19-11).
Figure 4.19-11 Location of Openings in the Baths Assembly Support Panel
8. From left to right, locate the tubing on the heater assembly and route the tubing through
the panel opening closest to the bath:
r Route the tubing attached to heater assembly port 2 through the first opening for
attachment to the DIL1/HGB bath.
r Route the tubing attached to heater assembly port 4 through the second opening for
attachment to the DIFF bath, DIFF 3.
r Route the tubing attached to heater assembly port 6 through the third opening for
attachment to the DIFF bath, DIFF 2.
PN 4237616B 4.19-7
r Route the tubing attached to heater assembly port 8 through the fourth opening for
attachment to the RBC bath, RBC 1.
r Route the tubing attached to heater assembly port 12 through the fifth opening for
attachment to the BASO bath, BASO 2.
9. Secure the two heater assembly screws (Figure 4.19-12).
Figure 4.19-12 Heater Assembly Screw Locations
10. At the Main card, reconnect the cable attached to the heater assembly to connector J33
(Figure 4.19-5).
11. Secure the reagent syringes and the 5diff syringe back inside the instrument:
a. Locate the six captive hex screws (Figure 4.19-1). The syringe assemblies should be
flush against the instrument frame.
ATTENTION: It is recommended that you use only four clockwise turns to tighten these
captive hex screws. As each hex screw is tightened, its rubber shock mount expands to
secure the syringe assembly to the instrument frame. If all Service Representatives
consistently use four turns to install these hex screws, it is unlikely that a rubber shock
mount will separate from its hex screw and fall inside the instrument the next time the
assembly is removed.
b. Tighten the six hex screws using four clockwise rotations.
12. Replace the front panel. Follow Heading 4.2, OPENING OR REMOVING INSTRUMENT
DOORS, PANELS, AND COVERS.
13. Do a probe to bath alignment check. Go to Heading 4.5, BATHS ASSEMBLY
ALIGNMENT CHECK AND ADJUSTMENT.
14. When startup and prime cycles are done, verify there are no leaks.
4.19-8 PN 4237616B
POWER SUPPLY REPLACEMENT 4
4.20 POWER SUPPLY REPLACEMENT
Purpose
Use this procedure to remove and replace the power supply as needed.
B Power Supply, PN 1xDBN 004 A
Removal
1. Turn the instrument off and disconnect the power cord.
2. Open the cover.
3. Open the left side door.
4. At the bottom of the Main card,
a. Disconnect the printer RS flat cable attached to connector J2 (Figure 4.20-1).
Figure 4.20-1 Main Card - J2 Location
b. With a flat-blade screwdriver, disconnect the power supply cable attached to

connector J37 (Figure 4.20-2).
PN 4237616B 4.20-1
POWER SUPPLY REPLACEMENT
Figure 4.20-2 Main Card - J37 Location
5. Open the Main card door (block it open).

6. Disconnect the optical bench lamp supply cable (Figure 4.20-3).
Figure 4.20-3 Optical Module - Lamp Supply Cable Location
7. At the rear panel of the instrument, remove the two CHC M3x6 screws securing the
power supply to the rear panel (Figure 4.20-4).
4.20-2 PN 4237616B
POWER SUPPLY REPLACEMENT 4
Figure 4.20-4 Location of Screws Securing the Power Supply to the Rear Panel
8. Remove the rear panel and set it aside.

9. Remove the two CHC M3x6 front screws securing the power supply to the instrument
frame (Figure 4.20-5).
Figure 4.20-5 Screws Securing the Power Supply to the Instrument Frame
CAUTION Make sure the lamp power supply cable is disconnected before removing the power
supply.
10. Remove the power supply.
Installation
1. Position the new power supply inside the instrument.
2. Secure the power supply to the instrument frame using the two CHC M3x6 front screws
removed earlier (Figure 4.20-5).
PN 4237616B 4.20-3
POWER SUPPLY REPLACEMENT
3. Make sure the lamp supply cable is routed towards the optical bench then replace the
rear panel.
4. Secure the power supply to the rear panel using the two CHC M3x6 screws removed
earlier (Figure 4.20-4).
5. Connect the optical bench lamp supply cable (Figure 4.20-3).
6. Close the Main card door.
7. At the bottom of the Main card,
a. Connect the power supply cable to connector J37 (Figure 4.20-2).
b. Connect the printer RS flat cable to connector J2 (Figure 4.20-1).
8. Insert the power supply connector into the socket on the rear instrument panel. Make
sure the plug end is in the wall socket.
Verification
At the Main card, check following voltages (Figure 4.20-6):
Table 4.20-1 Power Supply Voltages
Test Point Designation Target Voltage

TP40 5V Power supply +5 V
TP43 -12V Power supply -12 V
Figure 4.20-6 Main Card Heater Assembly Replacement Adjustment

T P 5
LMNE LMNE GR GB
T P 6 OD CIS RBC WBC
T P 7
T P 8
T P 9
T P 1 0
T P 1 1
T P 1 2
T P 4 0 T P 4 0 T P 4 1 T P 4 3
T P 4 0 T P 4 0 T P 4 1 T P 4 3
7616016B TP42 TP40 TP41 TP43
4.20-4 PN 4237616B
START SWITCH REPLACEMENT 4
4.21 START SWITCH REPLACEMENT
Purpose
Use this procedure to remove then replace the Start switch anytime it needs to be changed or
dismantled.
B Allen wrenches
B Torx keys
Removal
1. To access the start switch, remove the front panel. See Heading 4.2, OPENING OR
REMOVING INSTRUMENT DOORS, PANELS, AND COVERS, if needed.
3. Remove the enclosure fan that contains a thermostat (Figure 4.21-1).
a. Using a flat-blade screwdriver, remove the ground from the fan assembly.
b. Remove the four screws securing the fan to the front panel.
Figure 4.21-1 Fan Removal - Right Side Compartment
PN 4237616B 4.21-1
START SWITCH REPLACEMENT
4. Remove the two screws securing the start switch to the instrument’s frame
(Figure 4.21-2).
Figure 4.21-2 Start Switch Screw Locations - With Fan Removed
5. Disconnect the start switch (Figure 4.21-3).
Figure 4.21-3 Disconnected Start Switch - Front View with Front Panel Removed
4.21-2 PN 4237616B
START SWITCH REPLACEMENT 4
6. Remove the start switch.
Installation
IMPORTANT The Start switch is connected in the normally open position.
1. Connect the new start switch to J34 on the Main card.

2. Position the start switch (with the wheel at the top) in the two slots (Figure 4.21-4).
Figure 4.21-4 Start Switch Orientation
3. Install the two screws that secure the switch to the instrument frame.
4. With the front panel in position, verify that the start cycle key is operating normally.
r When the Start switch is installed correctly, you will hear a click when the sample
bar is pressed and another click when the sample bar is released.
r If you do not hear the two clicks, move the switch forward or backward until the
location is correct.
WARNING Use the washer to connect the ground wiring to the fan.
5. Reinstall the fan.

6. Reassemble the front panel. See Heading 4.2, OPENING OR REMOVING INSTRUMENT
DOORS, PANELS, AND COVERS, if needed.
PN 4237616B 4.21-3
START SWITCH REPLACEMENT
4.21-4 PN 4237616B
OPTICAL BENCH DISASSEMBLY AND REPLACEMENT 4
4.22 OPTICAL BENCH DISASSEMBLY AND REPLACEMENT
Purpose
Use this procedure to remove, replace, and control the optical bench assembly.
B Allen wrenches, 3.0 mm and 5.0 mm
Removal

ATTENTION: The two tubings disconnected from the 5diff syringe are attached to the flow cell.
To ensure proper reconnection, you may want to label each tubing with its port number
before disconnecting the tubing from the 5diff syringe assembly.
4. At the 5diff syringe, disconnect the tubings attached to ports 1 and 5.
Figure 4.22-1 5diff Syringe Port Locations

3
2
7616086A
4
PN 4237616B 4.22-1
OPTICAL BENCH DISASSEMBLY AND REPLACEMENT
5. At the 11-valve assembly,

a. Disconnect the tubing attached to solenoid valve 1, port 1.
b. Disconnect the tubing attached to solenoid valve 4, port 2.
6. Disconnect the tubing attached to the ground fitting (Figure 4.22-2).
Figure 4.22-2 Optical Bench - Ground Fitting Location
7. Disconnect the following components. See Figure 4.22-3 for disconnection locations
(from left to right):
r Lamp supply connector
r Optical Preamplifier card supply
r LMNE CIS and LMNE OD coaxes
r Optical bench grounding wire
Figure 4.22-3 Disconnection Sites for Named Components
4.22-2 PN 4237616B
ATTENTION: The optical bench assembly uses captive hex screws mounted inside rubber shock
screws. Turning the hex screw more than four counterclockwise rotations may separate the
screw from the rubber shock mount. If all Service Representatives consistently use four turns
to remove or install these hex screws, it is unlikely that a rubber shock mount will separate
from its hex screw and fall inside the instrument.
8. Locate the four hex screws circled in Figure 4.22-4. These are captive screws anchored
inside rubber shock mounts.
Figure 4.22-4 Captive Screw Locations - Optical Bench Assembly
9. Loosen each hex screw with four counterclockwise rotations (Figure 4.22-4). Use a
5.0 mm Allen wrench.
Note: If a screw is not released in four rotations, the screw was overtightened the last
time the optical bench assembly was serviced. From this point, make a single rotation
then check to see if the mount is free. Repeat this sequence of making a single rotation
and checking until the mount is free.
10. Gently remove the optical bench from the instrument.
Installation
1. Locate the new optical bench assembly and position it inside the instrument.
Note: Make sure the rubber shock mounts are positioned behind the panel. The optical
bench assembly should be flush against the instrument.
ATTENTION: It is recommended that you use only four clockwise turns to tighten the captive
hex screws. As each hex screw is tighten, its rubber shock mount expands to secure the
optical bench assembly to the instrument. If all Service Representatives consistently use four
turns to install these hex screws, it is unlikely that a rubber shock mount will separate from
its hex screw and fall inside the instrument the next time the assembly is removed.
Although you must be careful to not overtighten the rubber shock mounts, make sure the
optical bench is mounted securely. The assembly should not move if you try and lift it.
2. Tighten the four captive hex screws using four clockwise rotations (Figure 4.22-4). Use a
PN 4237616B 4.22-3
3. Reconnect the following components. See Figure 4.22-5 for connection locations
(from left to right):
r Lamp supply connector
r Optical Preamplifier card supply
r LMNE CIS and LMNE OD coaxes
r Optical bench grounding wire
Figure 4.22-5 Connection Sites for Named Components
4. Reconnect the tubing attached to the ground fitting (Figure 4.22-6).
Figure 4.22-6 Optical Bench - Ground Fitting Location
5. Reconnect the two hydraulic tubings as follows:

a. Attach the tubing connected to the isolator chamber to solenoid valve 1, port 1.
b. Attach the tubing connected to the flow cell (T-connector) to solenoid valve 4,
port 2.
4.22-4 PN 4237616B
6. At the 5diff syringe, reattach the two flow cell tubings to their proper ports, port 1 and
port 5.

3
2
7616086A
4
7. When the installation is completed, go to Heading 4.4, FLOW CELL CHECKS AND
ADJUSTMENTS to ensure proper flow cell operation.
PN 4237616B 4.22-5
4.22-6 PN 4237616B
BAR-CODE READER TESTING AND CONFIGURATION 4
4.23 BAR-CODE READER TESTING AND CONFIGURATION
Purpose
Use this procedure to test, troubleshoot, and reconfigure the bar-code reader.
Read Test
Verify the bar-code reader is working properly by successfully reading the test labels. That is,
when the bar-code reader is programmed to the default configuration, the reader successfully
reads all the labels in Table 4.23-1 and the Codabar label in Table 4.23-2.
If the bar-code reader fails to read the bar-code labels as specified, restore the reader to the
default settings, perform the Read Test again, then program each bar-code option per lab
requirements.
IMPORTANT There is a risk of sample misidentification if the entire bar code is not captured with the
bar-code reader, especially with the Interleaved 2-of-5 bar-code format. Position the bar-code reader over
the label to capture the entire bar-coded identification. Otherwise, part of the identification may not be
scanned, resulting in misidentification. Just as an operator must pass the bar-code reader over the bar-code
label on the specimen tube to capture the entire bar-coded sample ID, make sure the entire label is read to
avoid possible misidentification.
Table 4.23-1 Test Labels With Check Digit (Checksum)
Code 128 EAN 8

Reads 123456770
Code 39 EAN13
If this label is read with Check Digit disabled, Reads 12345678901228
the last character "$" is also displayed.
Interleaved 2-of-5
Reads 11 characters with Check Digit
or reads 12 characters without Check Digit.
PN 4237616B 4.23-1
BAR-CODE READER TESTING AND CONFIGURATION
Table 4.23-2 Test Labels Without Check Digit
Code 39 Codabar
Label will not read if scanner is programmed to
default condition
Default Settings
Do this procedure when the Read Test fails or you want to restore the default settings to the
bar-code reader. The default values are shown below.
Setting Code 128b Code 39 Codabar I 2-of-5 EAN 8 EAN 13

Character Length 1 to 16 1 to 16 3 to 16 11d 7 12
Check Digit (Checksum)c Always Enabled Not Enabled Always Always

Enabled Available Enabled Enabled
Start/Stop Equality Check Not Not Enabled Not Not Not
Available Available Available Available Available
Start/Stop Equality Output Not Not Disabled Not Not Not
Available Available Available Available Available
bCode 128 provides excellent density, alphanumeric characters, and good security. Recommend using this symbology if using
barcodes for the first time, and if compatible with other bar code systems used in your lab.
cFor increased sample identification integrity, always use Check Digit (Checksum).
dNumber of characters for I 2-of-5 can be programmed for other lengths, including variable length. However, the variable length is
NOT recommended for I 2-of-5 due to the possibility of capturing a partial read of the bar code label.
1. If the instrument is on, turn the instrument off then back on again before starting
programming.
2. Read in the bar-code labels in Table 4.23-3 from top to bottom and left to right. Bar codes
with $+ and $- will sound multiple beeps when read. Other codes will only sound a
single beep.
3. When the last label is read, do the Read Test again.
4.23-2 PN 4237616B
Table 4.23-3 Bar-code Labels for Default Configuration
PN 4237616B 4.23-3
Read in one of the Code 39 labels below to change the Check Digit option.
Code 39 Code 39
No Check Digit control Check Digit control
Codabar - Start/Stop Equality Check/Output

If you want to change the Start/Stop Equality Check/Output default setting (start/stop equality
check but no transmissions), read in one of the labels below.
No Start/Stop Equality Check No Start/Stop Equality Check

Nor Transmission But Transmission
Start/Stop Equality Check Start/Stop Equality Check

But No Transmission And Transmission
4.23-4 PN 4237616B
Interleaved 2-of-5 Options
If you want to change the Interleaved 2-of-5 default setting (check digit with 11 digits), read in
one of the labels for check digit control and select one of the labels to set the number of
digits.
Note: To increase sample identification integrity, use fixed length digits with Check Digit.
When check digit is enabled, the available digits are: 3, 5, 7, 9, 11, 13, 15, and variable
(3 to 15).
When check digit is disabled, the available digits are: 4, 6, 8, 10, 12, 14, 16 and variable
(4 to 16).
Variable length digits are NOT recommended for Interleaved 2-of-5 bar codes. If the test label
fails to read, reset the scanner by turning the instrument off then on and repeating the
programming sequence.
Number of
Digits With Check Digit Control No Check Digit Control Fixed Digit Test Labels
Read this label first, then ONE of Read this label first, then ONE of
the other labels below the other labels below
3* or 4†
5* or 6†
7* or 8†
PN 4237616B 4.23-5
Number of
Digits With Check Digit Control No Check Digit Control Fixed Digit Test Labels
9* or 10†
11* or 12†
13* or 14†
15* or 16†
[3 to 15]*
or
[4 to 15]†
*= With Check Digit Control

†= No Check Digit Control
4.23-6 PN 4237616B
REAGENT SYRINGES ASSEMBLY REPLACEMENTS 4
4.24 REAGENT SYRINGES ASSEMBLY REPLACEMENTS
Purpose
Use this procedure to remove and replace the reagent syringe pistons, O-rings, or washers.
The reagent syringes assembly consists of five reagent syringes. With the exception of the Hgb
Lyse syringe (the syringe with the smallest diameter), each syringe piston has both a washer
and an O-ring. The Hgb Lyse syringe has an O-ring but no washer.
When performing this procedure to replace an O-ring or a washer, replace both. When
replacing a syringe piston, replace its O-ring and washer as well.
B Torque screwdriver, 2.5 mm and T10 torx
Note: 400 mN.m (56.8 ozf.in) torque is required in this procedure.
B Absorbent paper towels
B Replacement parts, available as needed
r Washer and silicone O-ring, PN - XDA622A
Note: Four sets are needed when performing 6-months, 1-year, or every 2-years
maintenance.
r Reagent syringe piston (except Hgb Lyse), PN - GBC030A
Note: Four syringe pistons are needed when performing two-year maintenance.
r Hgb Lyse reagent syringe piston, PN - GBC031A
r Silicone O-ring for the Hgb Lyse reagent syringe piston, PN - FAA065A
r Silicone grease, PN - XEA019A
Preparation
2. From the Service menu, select 9. OTHERS tt 3. PARK SYRINGES.
4. Remove the left side door.
5. Open the Main card door and anchor the door so that it remains open.
PN 4237616B 4.24-1
REAGENT SYRINGES ASSEMBLY REPLACEMENTS
Removal
1. Disconnect the tubing attached to port 3 at valves 6, 7, 8, 9, and 11 (Figure 4.24-1):
Figure 4.24-1 Valve and Screw Locations - Left Side View
2. Remove the two hex screws securing the reagent syringes assembly (Figure 4.24-1). Use
a 3 mm Allen wrench.
3. Gently remove the reagent syringes assembly from the instrument.
4. Hold the reagent syringes assembly over a waste container and drain the syringes by
manually pushing the pistons up and down inside the syringe barrels.
Note: Selecting the PARK SYRINGES option at the beginning of this procedure, activated
an upward movement of the syringe pistons dispensing most of the reagent contained
inside the syringe. This manual up and down movement of the pistons is done to drain
residual reagent so that all syringes are relatively dry before proceeding.
5. Place the drained assembly on absorbent paper.
6. Wipe the solenoid valves with a lint-free tissue to remove any reagent that dripped on the
valves.
O-Ring, Washer, and Piston Replacement

If you are replacing an O-ring or washer, replace both. When replacing a syringe piston,
replace the O-ring and washer too. Complete needed replacements before proceeding to the
Installation instructions.
CAUTION With the exception of the Hgb Lyse reagent piston, each syringe piston has both a washer and an
O-ring. Compression of the O-ring is critical for a good piston seal. For this reason, the thickness of the
washer is matched to the O-ring thickness. As a result, it is important to keep the O-ring and its matching
washer together. The Hgb Lyse reagent syringe uses an O-ring but does not use a washer on its piston. See
Figure 4.24-2.
4.24-2 PN 4237616B
Figure 4.24-2 O-rings and Washers - Reagent Syringes Assembly
1. Remove the nine hex screws (CHC M3x12) and the two torx screws (FX M3x12) that
secure the bottom plate.
ATTENTION: When replacing parts, remove syringes one at a time and in order. Run
fingers along piston to check for scratches or scores. The Fix syringe may be slightly
discolored.
2. If you only need to replace an O-ring or washer, go to step 3. If you need to replace a
syringe piston, go to step 4.
3. One at a time, replace an old O-ring and washer as follows:
a. Remove and discard the old washer and O-ring.
Note: The Hgb Lyse reagent piston has an O-ring but does not have a washer.
b. Locate the replacement O-ring and washer.
c. Use a small amount of silicone grease between two fingers to lubricate the O-ring.
d. Place the O-ring on the syringe piston, followed by the washer (Figure 4.24-2).
Note: The Hgb Lyse reagent piston does not have a washer.
e. Repeat step 3 as many times as necessary then proceed to the IMPORTANT message
just before step 5.
4. One at a time, replace the old piston, O-ring, and washer as follows:
a. Remove and discard the old washer and O-ring.
Note: The Hgb Lyse reagent piston has an O-ring but does not have a washer.
b. Remove and discard the old syringe piston.
c. Clean the barrel inside the assembly with lint-free tissues.
d. Locate the replacement piston, O-ring, and washer.
e. Use a small amount of silicone grease between two fingers to lubricate the long
section of the piston.
f. Insert the lubricated piston into its barrel.
g. Use a small amount of silicone grease between two fingers to lubricate the new
O-ring.
PN 4237616B 4.24-3
h. Place the O-ring on the syringe piston, followed by the washer (Figure 4.24-2).
Note: The Hgb Lyse reagent piston does not have a washer.
i. Repeat step 4 as many times as necessary.
IMPORTANT Risk of misleading results. Restricted piston movement affects dilution ratios which may
affect final parameter results. The bottom plate on the reagent syringes assembly must be properly aligned
to ensure unrestricted movement of the syringe pistons. To ensure alignment, the bottom plate must be
installed in two stages with the two torx screws being installed first followed by installation of the nine hex
screws. These nine hex screws must be tightened in a zigzag, crisscross pattern to prevent skewing
(Figure 4.24-3).
5. When all reagent syringes assembly replacements are complete, replace the bottom plate
as follows:
a. Position the reagent syringes assembly so that the smallest piston is to your right, as
shown in Figure 4.24-2.
b. Carefully replace the bottom plate. Make sure all O-rings and washers are properly
seated inside their respective slots.
c. Loosely install the two torx screws - one screw in the center opening to the right of
the small piston; the second, in the upper left corner (Figure 4.24-3).
d. Loosely install the nine hex screws.
Figure 4.24-3 Bottom Plate Screw Locations and Tightening Patterns
Torx
screw
Torx
screw
7616052A
Start
ATTENTION: Torque needed for the two torx screws is 400 mN.m (56.8 ozf.in).
e. Tighten the two torx screws (Figure 4.24-3). Use a torque driver with a T10 torx bit
to tighten the screws to 400 mN.m (56.8 ozf.in).
4.24-4 PN 4237616B
ATTENTION: Torque needed for the nine hex screws is 400 mN.m (56.8 ozf.in). These hex
screws must be tighten in a zigzag, crisscross pattern to prevent skewing and ensure
proper alignment of the bottom plate (Figure 4.24-3).
f. Tighten the nine hex screws in a zigzag, crisscross pattern (Figure 4.24-3).
r Use a 2.5 mm torque screwdriver to tighten each screw to 400 mN.m
(56.8 ozf.in).
r Start in the lower left corner and use a zigzag pattern (from left to right) to
tighten every other screw. Then reverse the zigzag pattern. Start in the upper
right corner and use a right-to-left zigzag pattern to tighten the remaining loose
screws (as you crisscross the previous zigzag pattern).
Installation
1. Align the reagent syringes assembly back inside the instrument. Make sure the channel
on the back of the assembly housing fits securely on the motor housing guide.
2. Replace the two hex screws that secure the reagent syringes assembly to the instrument
(Figure 4.24-4). Use a 3 mm Allen wrench.
Figure 4.24-4 Valve and Screw Locations - Left Side View
3. Thread each tubing up through the hole and reconnect at port 3 of valves 6, 7, 8, 9 and
11 (Figure 4.24-4).
At the reagent syringes assembly, from left to right:
r Attach the first tubing (from the Hgb Lyse reagent syringe) to LV6, port 3.
r Attach the second tubing (from the Rinse reagent syringe) to LV7, port 3.
r Attach the third tubing (from the Fix reagent syringe) to LV8, port 3.
r Attach the fourth tubing (from the Diluent reagent syringe) to LV9, port 3.
r Attach the fifth tubing (from the WBC Lyse reagent syringe) to LV11, port 3.
Verification
PN 4237616B 4.24-5
3. When the startup routine and background check are done, verify the reagent syringes
assembly is not leaking.
4. If no leakage is seen, prime the reagents.
From the Main Menu, select 3. REAGENTS tt 3. PRIME tt 6. ALL REAGENTS.
5. When the prime cycles are done, check the reagent syringes assembly for leaks.
6. If no leaks are detected, close the Main card door and reattach the left side door.
PROCEDURES.
4.24-6 PN 4237616B
MAINTENANCE PROCEDURES
COUNT SYRINGE COMPONENT REPLACEMENTS 4
4.25 COUNT SYRINGE COMPONENT REPLACEMENTS
Purpose
Use this procedure to remove and replace the count syringe piston, O-ring, or washer. When
performing this procedure to replace an O-ring or a washer, replace both. When replacing the
count syringe piston, replace its O-ring and washer too.
B Allen wrenches, 2.5 mm, 3 mm
B T10 torx driver
B Replacement components, available as needed
r Washer and O-ring, PN - XDA621A
r Count syringe piston, PN - GBG052A
Preparation
3. Turn the instrument off.
6. At the rear of the instrument,
a. Disconnect the power cord.
b. Remove the six hex screws securing the rear access panel (Figure 4.25-1). Use a
3 mm Allen wrench.
Alignment
holes
Hex
7653001A
screws (6)
PN 4237616B 4.25-1
COUNT SYRINGE COMPONENT REPLACEMENTS
7. Remove the rear access panel and set it aside.
Removal
1. At the Motor Interconnect card (Figure 4.25-2):
a. Disconnect the large (unlabeled) cable connector from the J2 card connector on the
card. This allows better access to the individual motor and sensor connectors.
b. Disconnect sensor connector P7. In Figure 4.25-2, the dark arrow is pointing to this
connector. (The J7 connector on the card is oriented in a horizontal position behind
the large connector on the Motor Interconnect card. It is the second horizontal
connector from the top of the Motor Interconnect card.)
c. Disconnect motor connector P12. In Figure 4.25-2, the light arrow is pointing to
this connector. (The J12 connector on the card is oriented in a vertical position, the
second vertical connector from the top of the Motor Interconnect card.)
Figure 4.25-2 Motor Interconnect Card - Count Syringe Connector Locations

a. Locate the count syringe assembly.
b. Remove the ground wire from the instrument’s frame (Figure 4.25-3).
4.25-2 PN 4237616B
Figure 4.25-3 Count Syringe - Ground Wire Location
3. Place absorbent paper under the count syringe assembly and the 5-valve assembly to the
right of the syringe assembly.
4. Remove the four tubings attached to the fittings on the right side of the syringe housing
(Figure 4.25-4).
Figure 4.25-4 Count Syringe Housing - Tubing Locations
5. Locate the tubing attached to the bottom fitting. Trace this tubing to its connection point
on solenoid valve 16. Remove the tubing from LV16, port 1.
PN 4237616B 4.25-3
ATTENTION: The count syringe assembly uses captive hex screws mounted inside rubber shock
screws. Turning the hex screw too many counterclockwise rotations may separate the screw
from the rubber shock mount. If all Service Representatives consistently use four turns to
remove or install these hex screws, it is unlikely that a rubber shock mount will separate from
its hex screw and fall inside the instrument.
6. Locate the four hex screws (CHC M4x16) shown in Figure 4.25-5. These are captive
screws anchored inside rubber shock mounts. Use a 3 mm Allen wrench.
Figure 4.25-5 Count Syringe - Captive Screw Locations
7. Loosen each hex screw with four counterclockwise rotations (Figure 4.25-5).
time the count syringe assembly was serviced. From this point, make a single rotation
ATTENTION: Ensure the electronic cables are free and unrestricted as you remove the count
syringe assembly from the instrument. Do not continue to pull on the count syringe assembly
if these cables become restricted. Locate and free the restriction before proceeding.
8. Gently remove the count syringe from the instrument. Make sure the electronic cables
remain unrestricted.
9. Hold the count syringe assembly over a waste container and push the piston up and
down several times to drain the syringe barrel.
Note: Selecting the PARK SYRINGES option at the beginning of this procedure, activated
an upward movement of the syringe piston which expelled most of the reagent inside
this syringe. This manual up and down movement of the piston is done to drain residual
liquid so that the syringe is relatively dry before proceeding.
4.25-4 PN 4237616B
O-ring, Washer, and Piston Replacement
r If you only need to replace an O-ring on a piston or a washer on a housing, replace both
using the instructions under the O-ring and Washer Replacement Only heading.
r If you need to replace the syringe piston, replace the O-ring and washer too using the
instructions under the Piston Replacement heading.
Piston Replacement
1. Remove the four torx screws securing the syringe guide plate (Figure 4.25-6). Use a T10
torx driver.
Figure 4.25-6 Count Syringe - Piston, O-ring, and Washer Replacement
Torx Piston screws (3)

screws (4)
Ground
Shaft housing wire
Syringe
guide plate
O-ring
Syringe
Anti-extrusion
piston
ring (new style
assemblies only)
Washer
Syringe
cover
Syringe cover
screws (4) 7616113B
2. Remove the syringe guide plate.

3. Remove the four hex screws (CHC M4x16) located around the perimeter of the syringe
cover. Use a 3 mm Allen wrench.
4. Remove the cover to expose the syringe piston, O-ring, washer and anti-extrusion ring.
5. Remove and discard the O-ring and washer but retain the anti-extrusion ring.
ATTENTION: Be careful to keep the syringe drive nut in place on the motor lead screw.
6. Manually move piston up to expose the three piston screws.

7. Remove the three piston screws (CHC M3x16). Use a 2.5 mm Allen wrench (one screw
has the ground wire). Make sure the shaft housing remains stable.
8. Pull the piston off the shaft housing and remove it by passing it through the end of the
frame.
PN 4237616B 4.25-5
9. Replace the old piston, O-ring, and washer as follows (Figure 4.25-6):
a. Clean the barrel inside the syringe assembly with lint-free tissues.
b. Locate the replacement piston, O-ring, and washer.
c. Position the new piston in the housing and replace the three piston screws (with the
ground wire attached). Use a 2.5 mm Allen wrench.
d. Use a small amount of silicone grease between two fingers to lubricate the long
section of the piston and the new O-ring.
e. Place the O-ring on the syringe piston.
IMPORTANT Risk of erroneous results. The washer must be properly seated in the rim of the syringe
housing before reassembling the piston and housing.
f. Seat washer on rim of syringe housing followed by the anti-extrusion ring.

10. Replace the syringe cover. Make sure the notches on the cover and syringe are aligned.
Reinstall the four hex screws around the perimeter of the syringe cover. Use a 3 mm
Allen wrench. Make sure washer is not pinched.
11. Position the guide plate on the syringe assembly (notched corners toward the bottom)
then secure the plate to the assembly with the four torx screws. Use a T10 torx driver.
12. Go to the Installation heading.
O-ring and Washer Replacement Only

cover (Figure 4.25-7).
Figure 4.25-7 Count Syringe - O-ring and Washer Replacement
Anti-extrusion ring
(new style assemblies only)
Washer
O-ring
Syringe
piston
Syringe cover
screws (4)
Syringe
7616112B cover
4.25-6 PN 4237616B
4. Replace the old O-ring and washer as follows (Figure 4.25-7):
a. Locate the replacement O-ring and washer.
b. Use a small amount of silicone grease between two fingers to lubricate the O-ring.
c. Place the O-ring on the syringe piston.
d. Seat washer on rim of syringe housing followed by the anti-extrusion ring.

Installation
1. Position the count syringe assembly back inside the instrument.
Note: Make sure the rubber shock mounts are positioned behind the panel. The syringe
assembly should be flush against the instrument frame.
ATTENTION: It is recommended that you use only four clockwise turns to tighten these captive
syringe assembly to the instrument frame. If all Service Representatives consistently use four
3 mm Allen wrench.
3. Reattach the four tubings to the fittings on the right side of the syringe housing
(Figure 4.25-8).
From the top fitting to the bottom fitting, attach the tubings as follows:
r Attach the tubing connected to solenoid valve 13, port 1 to the top fitting on the
count syringe housing.
r Attach the tubing connected to solenoid valve 14, port 1 to the second fitting on the
r Attach the tubing connected to solenoid valve 23, port 2 to the third fitting on the
r Attach the tubing connected to solenoid valve 15, port 2 to the bottom fitting on the
4. Route the tubing attached to the bottom fitting of the syringe housing to solenoid
valve 16. Attach the tubing to port 1.
5. Reconnect the ground wire to the instrument frame (Figure 4.25-3).
6. Route the motor and sensor cables through the opening to the left of the count syringe
assembly. Route the connectors over to the Motor Interconnect card.
PN 4237616B 4.25-7
Figure 4.25-8 Count Syringe Housing - Tubing Locations
7. At the Motor Interconnect card (Figure 4.25-2):

a. Reattach motor connector P12. In Figure 4.25-2, the light arrow is pointing to this
connector. (The J12 connector on the card is oriented in a vertical position near the
top of the Motor Interconnect card).
b. Reattach sensor connector P7. In Figure 4.25-2, the dark arrow is pointing to this
the large connector on the Motor Interconnect card).
c. Reattach the large, unlabeled cable connector to the J2 card connector.
8. Replace the rear access panel using the six hex screws removed earlier (Figure 4.25-1).
Verification
1. Turn the instrument on. An automatic startup and background check are performed.
2. When the startup routine and background check are done, verify the count syringe is not
leaking.
PROCEDURES.
4.25-8 PN 4237616B
SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT REPLACEMENTS 4
4.26 SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT REPLACEMENTS
Purpose
Use this procedure to remove and replace the sample probe or any part of the rinse block
assembly including the probe guide, O-ring, or rinse block.
B Torque screwdriver, 2.5 mm, hex-ball
B Replacement parts, available as needed
r Sample probe, PN - XDA619AS
r Sample probe guide, PN - GBG091A
r O-ring, flurocarbon, PN - FAA053A
r Rinse block, PN - GBG090A
Preparation
Removal
ATTENTION: Note how the existing sample probe and rinse block assembly are seated.
1. Loosen the two hex screws (CHC M3x25) securing the sample probe rinse block
assembly on the traverse (Figure 4.26-1). Use a 2.5 mm hex-ball Allen wrench.
Figure 4.26-1 Probe Rinse Block Screw Locations
PN 4237616B 4.26-1
SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT REPLACEMENTS
2. Lift the sample probe lock-lever to free the probe then remove both the probe and rinse
block assembly at the same time (Figures 4.26-2 and 4.26-3).
Figure 4.26-2 Lift the Sample Probe Lock-Lever Figure 4.26-3 Remove Probe and Rinse Block Assembly
3. Separate the sample probe and the rinse block assembly.

r If the sample probe needs replaced, go to the Sample Probe Replacement heading.
r If one or more rinse block assembly components need replaced,
t Place the probe back on its support and close the lock-lever.
t Go to the Rinse Block Assembly Component Replacement heading.
Sample Probe Replacement

1. Remove the tubing attached to the top of the old probe.
2. Attach the new probe.
r If replacements in the rinse block assembly are required,
t Place the probe back on its support and close the lock.
t Go to the Rinse Block Assembly Component Replacement heading.
r If no other replacements are needed, go to the Installation heading.
Rinse Block Assembly Component Replacement

The rinse block assembly consists of three components: (from top to bottom) the probe
guide, a fluorocarbon O-ring, and the rinse block. Complete needed replacements before
proceeding to the Installation instructions.
4.26-2 PN 4237616B
SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT REPLACEMENTS 4
1. Remove the two hex screws.
2. Separate the rinse block assembly and replace components as needed. See step 3 to
replace the rinse block and step 4 to replace the O-ring or probe guide.
IMPORTANT Risk of erroneous results due to pinching of the tubing. When reattaching tubing from Valves
17 and 18 to new rinse block, route tubing from Valve 17 on inside of guide post, and tubing from Valve 18
around outside of guide post.
3. To replace the rinse block:

a. Place the new rinse block next to the old rinse block. Make sure the new rinse block
is oriented the same as the old with the top up and the ports towards the
instrument.
b. Remove tubing from the old rinse block. Route tubing from Valve 18 around outside
of guide post and attach to port 1 on the new rinse block. Route tubing from Valve
17 on inside of guide post and attach to port 2 on the new rinse block.
4. To replace the O-ring:
a. Remove and discard the old O-ring.
b. Clean then dry the two white blocks. Use a twisted lint free tissue to clean the
center opening.
c. Locate the replacement O-ring.
d. Use a small amount of silicone grease between two fingers to lubricate the O-ring.
e. Place the new O-ring inside the probe guide (top block).
Note: Use a new probe guide if needed.
5. When all rinse block component replacements are complete,
a. Reassemble the rinse block (Figure 4.26-4).
Figure 4.26-4 Reassemble the Rinse Block Assembly
b. Route the two hex screws through the openings at the top of the assembly.
c. Proceed to the Installation heading to complete this procedure.
PN 4237616B 4.26-3
SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT REPLACEMENTS
Installation
1. Lift the probe lock-lever and remove the probe from its support (Figure 4.26-2).
2. Position the probe inside the probe guide, using care not to bend the probe.
3. Lift the probe lock and return the probe and the rinse block assembly back in its original
position on the traverse. Position the notch and tubing towards the front of the
instrument.
4. Seat the collar of the sample probe by gently pushing it into place then close the
lock-lever.
ATTENTION: Torque needed for the rinse block assembly screws is 100 mN.m (14.2 ozf.in).
5. Tighten the two hex screws to secure the rinse block assembly to the traverse carriage
(Figure 4.26-1). Use a 2.5 mm, hex-ball torque screwdriver to tighten the screws to
100 mN.m (14.2 ozf.in).
Verification
2. When the startup routine and background check are done, verify the sample probe and
the rinse block assembly are not leaking.
PROCEDURES.
4.26-4 PN 4237616B
WASTE SYRINGE COMPONENT REPLACEMENTS 4
4.27 WASTE SYRINGE COMPONENT REPLACEMENTS
Purpose
Use this procedure to remove and replace the waste syringe piston, O-ring, or washer. When
performing this procedure to replace an O-ring or a washer, replace both. When replacing the
waste syringe piston, replace its O-ring and washer as well.
B Cutting pliers
B T10 torx driver
B Replacement components, available as needed
r Washer and O-ring, PN - XDA621A
r Waste syringe piston, PN - GBG052A
Preparation
b. Remove the six hex screws securing the rear access panel (Figure 4.27-1). Use a
3 mm Allen wrench.
Alignment
holes
Hex
3001A
screws (6)
PN 4237616B 4.27-1
WASTE SYRINGE COMPONENT REPLACEMENTS
5. Remove the rear access panel and set it aside.
Removal
1. At the Motor Interconnect card (Figure 4.27-2),
a. Disconnect the large, unlabeled cable connector from the J2 card connector. This
allows better access to the individual motor and sensor connectors.
b. Disconnect sensor connector P8. In Figure 4.27-2, the dark arrow is pointing to this
the large connector on the Motor Interconnect card.)
c. Disconnect motor connector P13. In Figure 4.27-2, the light arrow is pointing to
this connector. (The J13 connector on the card is oriented in a vertical position near
the top of the Motor Interconnect card.)
Figure 4.27-2 Motor Interconnect Card - Waste Syringe Connector Locations

a. Locate the waste syringe assembly.
b. Cut the tie wrap (Figure 4.27-3).
a. Remove the ground wire from the instrument’s frame.
4.27-2 PN 4237616B
Figure 4.27-3 Waste Syringe - Tie Wrap and Ground Wire Location
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the waste
syringe assembly, you may become contaminated. To prevent possible biological contamination, you must
wear appropriate safety glasses, a lab coat, and gloves when servicing the waste syringe assembly.
3. Place absorbent paper under the waste syringe and the 7-valve assembly to its left.
4. At the waste syringe, disconnect the two tubings as follows:
a. Remove the tubing attached to the left side fitting (port 1).
b. Locate the tubing attached to the bottom fitting (port 2). Trace this tubing to its
connection point on LV26. Remove the tubing from LV26, port 3.
Note: To minimize waste exposure, use a hemostat to clamp this tubing between the
7-valve assembly and the waste syringe before removing the tubing from LV26.
ATTENTION: The waste syringe assembly uses captive hex screws mounted inside rubber shock
screws. Turning the hex screw more than four counterclockwise rotations may separate the
screw from the rubber shock mount. If all Service Representatives consistently use four turns
to remove or install these hex screws, it is unlikely that a rubber shock mount will separate
from its hex screw and fall inside the instrument.
5. Locate the four hex screws (CHC M4x16) circled in Figure 4.27-4. These are captive
screws anchored inside rubber shock mounts. Use a 3 mm Allen wrench.
PN 4237616B 4.27-3
Figure 4.27-4 Waste Syringe - Captive Screw Locations
6. Loosen each hex screw with four counterclockwise rotations (Figure 4.27-4).
time the waste syringe assembly was serviced. From this point, make a single rotation
ATTENTION: Ensure the electronic cables are free and unrestricted as you remove the waste
syringe assembly from the instrument. Do not continue to pull on the waste syringe assembly
if these cables become restricted. Locate and free the restriction before proceeding.
7. Gently remove the waste syringe from the instrument. Make sure the electronic cables
remain unrestricted.
4.27-4 PN 4237616B
8. Hold the waste syringe assembly over a biohazardous waste container and push the
piston up and down several times to drain the syringe barrel.
Piston Replacement
1. Remove the four torx screws securing the syringe guide plate (Figure 4.27-5). Use a T10
torx driver.
Figure 4.27-5 Waste Syringe - Piston, O-ring, and Washer Replacement

Torx Piston screws (3)
screws (4)
Ground
Shaft housing wire
Syringe
guide plate
O-ring
Syringe
Anti-extrusion
piston
ring (new style
assemblies only)
Washer
Syringe
cover
Syringe cover
screws (4) 7616113B
2. Remove the syringe guide plate.

cover. Use a 3 mm Allen wrench.
ATTENTION: Be careful to keep the syringe drive nut in place on the motor lead screw.
6. Manually move piston up to expose the three piston screws.

7. Remove the three piston screws (CHC M3x16). Use a 2.5 mm Allen wrench (one screw
has the ground wire). Make sure the shaft housing remains stable.
8. Pull the piston off the shaft housing and remove it by passing it through the end of the
frame.
PN 4237616B 4.27-5
9. Replace the old piston, O-ring, and washer as follows (Figure 4.27-5):
a. Clean the barrel inside the syringe assembly with lint-free tissues.
b. Locate the replacement piston, O-ring, and washer.
c. Position the new piston in the housing and replace the three piston screws (with the
ground wire attached). Use a 2.5 mm Allen wrench.
d. Use a small amount of silicone grease between two fingers to lubricate the long
section of the piston and the new O-ring.
e. Place the O-ring on the syringe piston.
f. Seat washer on rim of syringe housing followed by the anti-extrusion ring.

11. Position the guide plate on the syringe assembly (notched corners toward the bottom)
then secure the plate to the assembly with the four torx screws. Use a T10 torx driver.
12. Go to the Installation heading.
O-ring and Washer Replacement Only

cover (Figure 4.27-6).
Figure 4.27-6 Waste Syringe - O-ring and Washer Replacement
Anti-extrusion ring
(new style assemblies only)
Washer
O-ring
Syringe
piston
Syringe cover
screws (4)
Syringe
7616112B cover
4.27-6 PN 4237616B
4. Replace the old O-ring and washer as follows (Figure 4.27-6):
a. Locate the replacement O-ring and washer.
b. Use a small amount of silicone grease between two fingers to lubricate the O-ring.
c. Place the O-ring on the syringe piston.
d. Seat washer on rim of syringe housing followed by the anti-extrusion ring.

Installation
1. Position the waste syringe assembly back inside the instrument.
Note: Make sure the rubber shock mounts are positioned behind the panel. The syringe
assembly should be flush against the instrument frame.
ATTENTION: It is recommended that you use only four clockwise turns to tighten these captive
syringe assembly to the instrument frame. If all Service Representatives consistently use four
3 mm Allen wrench.
3. Reconnect the two hydraulic tubings as follows:
a. Attach the tubing connected to S20 (port 1) to the left side fitting (port 1) on the
waste syringe assembly.
b. Route the tubing attached to the bottom fitting (port 2) to S26. Attach the tubing
(with the collar) to port 3.
4. Reconnect the grounding wire to the instrument frame (Figure 4.27-3).
5. Route the motor and sensor cables through the opening to the left of the waste syringe
assembly (Figure 4.27-3). Route the connectors over to the Motor Interconnect card.
PN 4237616B 4.27-7
6. At the Motor Interconnect card (Figure 4.27-2),

a. Reattach motor connector P13. In Figure 4.27-2, the light arrow is pointing to this
connector. (The J13 connector on the card is oriented in a vertical position near the
top of the Motor Interconnect card).
b. Reattach sensor connector P8. In Figure 4.27-2, the dark arrow is pointing to this
the large J2 connector on the Motor Interconnect card).
c. Reattach the large unlabeled cable connector to the J2 card connector.
7. Replace the rear access panel using the six hex screws removed earlier (Figure 4.27-1).
Verification
1. Turn the instrument on. An automatic startup and background check are performed.
2. When the startup routine and background check are done, open the right side door and
verify the waste syringe is not leaking.
3. If no leaks are detected, perform a system verification. See Heading 5.3, SYSTEM
VERIFICATION PROCEDURES.
4.27-8 PN 4237616B
CLEANING THE BATH ENCLOSURE 4
4.28 CLEANING THE BATH ENCLOSURE
Purpose
Use this procedure to clean bath enclosure parts with diluted bleach. This procedure provides
instructions for cleaning the:
r Black plastic that covers the baths (attached to the right side door).
r White plastic under the baths.
r Top of the hemoglobin bath.
B Deionized water
B Lint-free tissues
Procedure
1. Prepare a 1:5 bleach solution: 4 parts deionized water to 1 part high quality,
fragrance-free bleach (10-12% sodium hypochlorite - available chloride).
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the waste
wear appropriate safety glasses, a lab coat, and gloves when servicing the waste syringe assembly.
ATTENTION: Do not use a sponge or cloth to clean instrument assemblies. Use absorbent paper
towels that can be discarded in a biohazardous container. For small or sensitive assemblies
such as the top of the hemoglobin bath, use lint-free tissues.
4. Pour the diluted bleach on a soft paper towel and thoroughly clean the black plastic that
covers the baths (attached to the right side door) and the white plastic under the baths.
5. Wipe the plastic dry with a clean paper towel.
6. Discard the towels in a biohazardous container.
7. Wet a lint-free tissue with the diluted bleach and clean the top of the hemoglobin bath.
8. Wipe the top of the bath dry with a clean lint-free tissue.
9. Discard the tissues in a biohazardous container.
10. Close the pneumatic access door (right side of the instrument).
11. Turn the instrument on. An automatic startup and background check is performed.
12. When the startup routine and background check are done, resume normal operation.
PN 4237616B 4.28-1
CLEANING THE BATH ENCLOSURE
4.28-2 PN 4237616B
5diff SYRINGE ASSEMBLY REPLACEMENTS 4
4.29 5diff SYRINGE ASSEMBLY REPLACEMENTS
Purpose
The 5diff syringe consists of one large center piston with a smaller injector piston on each
side. Use this procedure to remove and replace the 5diff syringe O-rings associated with this
center piston and the two injector pistons. Two types of O-rings are used in the 5diff syringe.
The large silicone O-ring is used for the center piston and two smaller fluorocarbon O-rings
are used on each injector piston. Although this procedure focuses on O-ring replacement, it
could be used as a guide in replacing any component inside the 5diff syringe housing or for
replacing the entire 5diff syringe assembly.
B Torque screwdriver, 2.5 mm and T10 torx
B Replacement components
r Silicone O-ring, PN - FAA040A
r Four fluorocarbon O-rings, PN - FAA067A
Preparation
4. Remove the left side panel from the instrument. For details, see Heading 4.2. Set the
panel aside.

Removal
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the 5diff
wear appropriate safety glasses, a lab coat, and gloves when servicing the 5diff syringe assembly.
1. At the 5diff syringe assembly, disconnect the two tubings associated with the flow cell:
a. Disconnect the tubing attached to port 1 (Figure 4.29-1).
b. Disconnect the tubing attached to port 5 (Figure 4.29-1).
PN 4237616B 4.29-1
5diff SYRINGE ASSEMBLY REPLACEMENTS

3
2
7616086A
4
2. Disconnect the other three tubings at the 11-valve assembly (Figure 4.29-2):
a. Disconnect the tubing attached to valve 2, port 2.
(The other end of this tubing is attached to 5diff syringe port 4, the lower left port.)
b. Disconnect the tubing attached to valve 3, port 3.
(The other end of this tubing is attached to 5diff syringe port 2, upper middle port.)
c. Disconnect the tubing attached to valve 5, port 1.
(The other end of this tubing is attached to 5diff syringe port 3, upper right port.)
Figure 4.29-2 Valve and Tubing Locations - Left Side View
4.29-2 PN 4237616B
3. Remove the two screws on the front of the 5diff syringe housing (Figure 4.29-3). Use a
Figure 4.29-3 Screw Locations - 5diff Syringe
4. Remove the 5diff syringe assembly.

5. Place the syringe assembly on absorbent paper.
O-ring Replacements
1. At the bottom plate (Figure 4.29-4),
a. Remove the two torx screws (FX M3x12) that are flush with the bottom plate. Use a
T10 torx driver.
b. Remove the four hex screws (CHC M3x12) using a 2.5 mm Allen wrench.
Figure 4.29-4 Bottom Plate - 5diff Syringe
PN 4237616B 4.29-3
2. Separate the bottom plate (and attachments) from the 5diff syringe housing
(Figure 4.29-5).
Figure 4.29-5 Bottom Plate and Attachments - Housing Removed
3. Clean then dry the outside of the 5diff syringe housing.

4. Replace the O-rings on the 190 µL injector piston located on each side of the center
syringe piston (Figure 4.29-6):
a. Remove the two O-rings and the collar from each injector piston.
b. Discard the four old O-rings. Save the two collars.
c. Clean the outside of the injector pistons, the center piston, and the bottom plate.
d. Locate the four replacement fluorocarbon O-rings.
e. Use a small amount of silicone grease between two fingers to lubricate an O-ring
then place it on the injector piston.
f. Place the collar on the injector piston.
g. Use a small amount of silicone grease between two fingers to lubricate another
O-ring then place it on the injector piston.
h. Repeat steps e through g to replace the O-rings on the other injector piston.
4.29-4 PN 4237616B
Figure 4.29-6 Illustrated Parts - 5diff Syringe
Housing
Silicone O-ring
Center piston Flurocarbon O-ring

(outer sheath)
Collar
Flurocarbon O-ring
Bottom Torx screw

plate
Hex screw
190 µL Injector piston

190 µL Injector piston
(flow cell sample)
(inner sheath) 7616055B
5. Use a small amount of silicone grease between two fingers to lubricate the silicone
O-ring before installing this O-ring on the syringe piston.
6. Once all replacements are complete, replace the bottom plate as follows (Figure 4.29-6):
a. Carefully replace the bottom plate. Make sure the piston O-ring is properly seated
inside the slotted opening on the bottom plate.
b. Loosely install the two torx screws in the two outside openings.
c. Loosely install the four hex screws.
ATTENTION: Torque needed for the two torx screws is 400 mN.m (56.8 ozf.in).
d. Tighten the two torx screws. Use a torx driver with a T10 torx bit to tighten the
screws to 400 mN.m (56.8 ozf.in).
ATTENTION: Torque needed for the four hex screws is 400 mN.m (56.8 Ozf.in). These hex
screws must be tightened in a crisscross pattern to prevent skewing and ensure
alignment of the bottom plate.
e. Tighten the four hex screws in a crisscross pattern.
r Use a 2.5 mm torque screwdriver to tighten each screw to 400 mN.m
(56.8 ozf.in).
r Start by tightening the lower left hex screw then move to the upper right.
Continue the process by tightening the upper left hex screw then move to the
lower right.
PN 4237616B 4.29-5
Installation
1. Position the 5diff syringe assembly back inside the instrument. Make sure the channel
on the back of the assembly housing fits securely on the motor housing guide. Use a
3 mm Allen wrench.
2. Replace the two hex screws that secure the 5diff syringe assembly to the instrument.
3. At the 5diff syringe assembly, attach the two tubings associated with the flow cell
(Figure 4.29-1):
a. Connect the loose tubing near the top of assembly to port 1 (upper left port).
b. Connect the loose tubing near the bottom of the assembly to port 5 (lower right
port).
4. Attach the other three tubings at the 11-valve assembly (Figure 4.29-2):
a. Connect the tubing (with a collar) that is attached to the upper middle port (port 2)
on the 5diff syringe assembly to valve 3, port 3.
b. Connect the tubing that is attached to the upper right port (port 3) on the 5diff
syringe assembly to valve 5, port 1.
c. Connect the tubing that is attached to the lower left port (port 4) on the 5diff
syringe assembly to valve 2, port 2.
Verification
3. When the startup routine and background check are done, verify the 5diff syringe is not
leaking.
PROCEDURES.
4.29-6 PN 4237616B
FLOW CELL COAXIAL CABLE REPLACEMENT 4
4.30 FLOW CELL COAXIAL CABLE REPLACEMENT
Purpose
Use this procedure to replace the flow cell coaxial cable located inside the optical bench.
B Small Phillips-head screwdriver
B Lint-free tissues
B DIFF flow cell coaxial cable, PN - XBA399A
Preparation
2. Remove the left side panel and top cover from the instrument. For details, see
Heading 4.2. Set the top cover and left side panel aside.

Removal
1. Remove the four screws securing the optical bench cover (Figure 4.30-1).
Figure 4.30-1 Optical Bench Cover Screw Locations
PN 4237616B 4.30-1
FLOW CELL COAXIAL CABLE REPLACEMENT
2. Locate the T-connector inside the optical bench (Figure 4.30-2).

Note: Take time to examine the T-connector and its attachments. Notice a cable is
inserted in the base of the T-connector. The top of the T-connector links two liquid
tubings - the tubing from the isolator chamber and the tubing to the flow cell.
Figure 4.30-2 T-Connector Location
IMPORTANT Risk of compromising results if liquid splashes on the surface of the flow cell. Wet or dried
liquid on the flow cell can affect output. Do not allow liquid to splash on the surface of the flow cell.
3. While holding a few lint-free tissues under the T-connector, disconnect the tubing
coming from the isolator chamber (Figure 4.30-3). A few diluent drops will leak out.
4. Verify the sleeving is still attached to the tubing. If not, remove the sleeving from the
T-connector and place it back on the tubing. This sleeve is needed to prevent leakage.
Figure 4.30-3 Disconnecting the Isolator Chamber from the T-connector
4.30-2 PN 4237616B
ATTENTION: Do not remove the tubing directly attached to the flow cell.
5. On the other side of the T-connector (at the T-connector, not the flow cell), disconnect
the tubing going to the flow cell.
6. Verify the sleeving is still attached to the tubing. If not, remove the sleeving from the
T-connector and place it back on the tubing. This sleeve is needed to prevent leakage.
7. Disconnect the coaxial cable from the coaxial connector on the optical bench housing.
Figure 4.30-4 Coaxial Cable Connector - Top View
IMPORTANT Risk of compromising results if the flow cell surface is smudged. Fingerprints or other
smudges on the flow cell can affect output. Do not touch the surface of the flow cell. Handle the black
mount only when moving the flow cell.
8. Disconnect the ground fitting under the flow cell as follows:

a. Locate and remove the two screws shown in Figure 4.30-5. Use a 3 mm Allen
wrench.
Figure 4.30-5 Flow Cell Screw Locations - Top View
b. Using care not to touch the surface of the flow cell, slide the flow cell forward to
separate it from its mount.
c. Turn the flow cell slowly. Make sure the plastic shims remain in the instrument.
PN 4237616B 4.30-3
d. Remove the ground screw using a small Phillips-head screwdriver (Figure 4.30-6).
Figure 4.30-6 Ground Screw Removal
Replacement
1. Connect the ground ring on the new DIFF coaxial cable to the flow cell as follows:
a. Position the ground ring on the bottom of the flow cell assembly and secure with
the screw removed earlier. Use a small Phillips-head screwdriver (Figure 4.30-6).
b. Turn the flow cell slowly and slide it inside its housing.
c. Install the two screws that secure the flow cell to the optical bench (Figure 4.30-5).
Use a 3 mm Allen wrench.
2. Attach the coaxial cable to the coaxial connector on the optical bench housing
(Figure 4.30-7).
3. Attach the new DIFF coaxial cable, at the T-connector (Figure 4.30-7):
Note: These tubings are sleeved to prevent leakage.
a. Insert the tubing and sleeve from the isolator chamber into one leg of the
T-connector.
b. Insert the tubing and sleeve from the flow cell into the other leg of the T-connector.
4.30-4 PN 4237616B
Figure 4.30-7 Connector Location - Top View

5. Turn the instrument on. An automatic Startup and background check is performed.
6. When the Startup routine and background check are done, press ESC to display the Main
Menu.
7. From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEMS tt 2. RINSE tt
2. FLOWCELL to get rid of the air bubbles stuck to the inner optical surfaces.
8. When the flow cell routine is done, verify there are no leaks and that the flow cell
contains no (or just a very few) air bubbles.
9. Replace the optical bench cover (Figure 4.30-1).
Verification
Go to Heading 4.4 and do a flow cell adjustment check. If an adjustment value is outside the
acceptable range, do the corresponding adjustment.
PN 4237616B 4.30-5
4.30-6 PN 4237616B
OPTICAL BENCH LAMP REPLACEMENT 4
4.31 OPTICAL BENCH LAMP REPLACEMENT
Purpose
Use this procedure to replace the optical bench lamp when the flow cell lamp fails.
B Allen wrenches, 3 mm, 2.5 mm, 2 mm
B Optical bench lamp. PN - DAJ007A
Preparation
2. Remove the left side panel and top cover from the instrument. For details, see

WARNING Risk of personal injury due to hot surfaces within the instrument. Use care when working in this
area. The lamp and some of the surfaces may be very hot and can burn you.
4. At the rear of the optical bench assembly (as view from the front of the instrument),
locate the existing lamp and examine how it is seated. Figure 4.31-1 shows an exploded
view of the assembly.
PN 4237616B 4.31-1
OPTICAL BENCH LAMP REPLACEMENT
Figure 4.31-1 Optical Bench Lamp Assembly
Winged metal
bracket
Keyed metal
bracket
Lamp
Squared
lock
Rounded
lock
7616097A
5. Notice the positioning of the two metal brackets (Figure 4.31-1):

r The top metal “winged” bracket is secured underneath a screw near each end.
r The bottom metal bracket, which is part of the lamp, is keyed to ensure proper
positioning of the lamp. Note the two different notches:
t One notch is a semicircle that matches the rounded raised area (lock) on the
housing.
t On the opposite side is a squared notch that matches a squared raised area
(lock) on the housing.
4.31-2 PN 4237616B
Removal
WARNING Risk of personal injury due to hot surfaces within the instrument. Use care when working in this
area. The lamp and some of the surfaces may be very hot and can burn you.
1. At the power connector, disconnect the lamp from the power supply (Figure 4.31-2).
Figure 4.31-2 Power Connector Location - Top View
2. At the top of the lamp housing, loosen the two screws a few turns (Figure 4.31-3). It is
not necessary to remove the screws. Use a 2 mm hex key.
Figure 4.31-3 Screw Locations - Lamp Housing
PN 4237616B 4.31-3
3. Turn the winged metal bracket counterclockwise to unlock the lamp (Figure 4.31-4).
Figure 4.31-4 Winged Metal Bracket - Top View
4. Lift the lamp and the bracket out of the housing.

5. Separate the winged metal bracket from the lamp and cable assembly.
6. Save the winged metal bracket. Discard the old lamp assembly.
Lamp Replacement
IMPORTANT Risk of compromising output of the new lamp if the surface is smudged. Fingerprints or other
smudges on the lamp can affect output. Do not touch the surface of the lamp.
1. Using care not to touch the surface of the new lamp, place the old winged metal bracket
over the new lamp.
2. Using the notches on the new lamp as a guide, position the new lamp assembly inside
the housing. When the positioning is correct, each notch fits snugly against its raised
area - like a lock and key.
3. Turn the winged metal bracket clockwise until it is seated under the two screws.
4. Tighten the two screws (Figure 4.31-3).
5. Reconnect the lamp to the Power Supply (Figure 4.31-2).
4.31-4 PN 4237616B
Verification
4. While the instrument is performing a Startup and background check, verify the new
lamp is lighted.
5. When the Startup routine and background check are done, replace all covers and close
all doors.
a. Gently close the Main card door. Turn the two captive knobs clockwise to secure the
door.
b. Replace the top cover and install the five hex screws that secure it to the instrument
frame. See Heading 4.2 as needed.
frame.
c. Replace the left side panel and install the four hex screws that secure it to the
instrument frame.
d. Close the right side door.
PROCEDURES
PN 4237616B 4.31-5
4.31-6 PN 4237616B
DILUENT RESERVOIR REPLACEMENTS 4
4.32 DILUENT RESERVOIR REPLACEMENTS
Purpose
Use this procedure to replace the O-ring and washer in the diluent reservoir. When
performing this procedure to replace an O-ring or washer, replace both.
B Torque screwdriver, 2.5 mm hex-ball
B O-ring and washer, PN - XEA286AS
Preparation
2. From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEMS tt 3. DRAIN BATHS tt
7. DILUENT RESERVOIR.
3. When the cycle is finished, open the right side door and verify the diluent reservoir is
empty.
Removal
1. Locate the tubing attached to the bottom fitting under the reservoir tank. Trace this
tubing to its connection point on solenoid valve 25. Remove the tubing from LV25,
port 1.
2. Loosen the two screws under the diluent reservoir (Figure 4.32-1). It only takes a few
turns to release the diluent reservoir from its support. Use a 3.0 mm Allen key.
Figure 4.32-1 Diluent Reservoir Screw Locations
PN 4237616B 4.32-1
DILUENT RESERVOIR REPLACEMENTS
3. Carefully remove the reservoir and bottom bracket. Be careful that no other tubings
disconnect from the reservoir.
O-ring and Washer Replacement

1. Remove the four screws on top of the cap using a 2.5 mm Allen wrench then remove the
cap.
2. Remove and discard the old O-ring and washer.
3. Clean then dry the cap and the reservior.
4. Locate the replacement O-ring and washer.
5. Use a small amount of silicone grease between two fingers to lubricate the O-ring.
6. Place the new washer and O-ring securely inside the reservior cap (Figure 4.32-2).
Figure 4.32-2 Diluent Reservoir O-ring and Washer Positioning
7. Position the cap back on the reservoir body with the interior support connection turned
towards the inside of the instrument and the extended tubings in the front
(Figure 4.32-2).
8. When the cap is in position, place the four reservoir screws back in the cap then loosely
tighten the four screws.
ATTENTION: Torque needed for the four hex screws (CHC M3x20) is 120 mN.m (17.0 ozf.in).
9. Tighten the four hex screws to secure the cap to the reservoir. Use a 2.5 mm hex-ball
torque screwdriver to tighten the screws to 120 mN.m (17.0 ozf.in).
4.32-2 PN 4237616B
DILUENT RESERVOIR REPLACEMENTS 4
Installation
1. Position the diluent reservoir and bracket back in its original location.
2. Tighten the two hex screws that attach the reservior to its support (Figure 4.32-1).
3. Reattach the drain tubing under the diluent reservoir to solenoid valve 25, port 1.
Verification
4. When the startup routine and background check are done, verify the diluent reservoir is
not leaking.
PN 4237616B 4.32-3
DILUENT RESERVOIR REPLACEMENTS
4.32-4 PN 4237616B
SAMPLE SYRINGE ASSEMBLY REPLACEMENTS 4
4.33 SAMPLE SYRINGE ASSEMBLY REPLACEMENTS
Purpose
Use this procedure to remove and replace the two O-rings on the injector piston of the sample
syringe.
B Torque screwdriver, T10 torx
B Replacement parts: O-ring, FAA064A (need 2)
Preparation
Removal
1. Manually push the sample probe housing towards the rear of the instrument until the
sample probe is positioned over the rinse chamber.
2. Disconnect the tubing at solenoid valve 18, port 2 (Figure 4.33-1). The diluent drains
through the sample syringe into the rinse chamber.
Figure 4.33-1 Disconnect Tubing at Valve 18, Port 2
PN 4237616B 4.33-1
SAMPLE SYRINGE ASSEMBLY REPLACEMENTS
3. Remove the two hex screws securing the sample syringe housing to the instrument frame
Figure 4.33-2 Screw Locations - Sample Syringe Housing
4. Disconnect the tubing attached to the syringe output port (Figure 4.33-3). The other end
of this tubing is connected to the top port of the sample probe.
Figure 4.33-3 Sample Syringe Output Port
5. Remove the housing from the instrument and place it on absorbent paper towels.
4.33-2 PN 4237616B
O-ring Replacements
1. At the bottom plate, remove the three torx screws (FX M3x12) using a T10 Torx
screwdriver (Figure 4.33-4).
Figure 4.33-4 Screw Locations on the Bottom of the Sample Syringe Housing
2. Separate the bottom plate (and attachments) from the sample syringe housing.
3. Clean then dry the outside of the sample syringe.
4. Replace the O-rings on the injector piston (Figure 4.33-5):
a. Remove the two O-rings and the collar from the injector piston.
b. Discard the old O-rings. Save the collar.
c. Clean the outside of the injector piston and the bottom plate.
d. Locate the two replacement O-rings.
e. Use a small amount of silicone grease between two fingers to lubricate an O-ring
then place it on the injector piston.
f. Place the collar on the injector piston.
g. Use a small amount of silicone grease between two fingers to lubricate another
O-ring then place it on the injector piston.
PN 4237616B 4.33-3
Figure 4.33-5 Illustrated Parts - Sample Syringe
Housing
O-ring
Collar
O-ring
Bottom plate
Torx screw
Injector piston
7616098A
5. Once the replacements are complete, replace the bottom plate as follows:
a. Carefully replace the bottom plate.
b. Loosely install the three torx screws (Figure 4.33-4).
ATTENTION: Torque needed for the three torx screws is 400 mN.m (56.8 ozf.in).
c. Tighten the three torx screws. Use a torx driver with a T10 torx bit to tighten the
screws to 400 mN.m (56.8 ozf.in).
Installation
1. Position the sample syringe back inside the instrument.
2. Replace the two hex screws that secure the sample syringe to the instrument
(Figure 4.33-2).
3. Reattach the tubing connected at the top of the sample probe to the output port on the
top of the syringe housing.
4. Reattach the tubing connected at the side of the sample syringe to solenoid valve 18,
port 2.
4.33-4 PN 4237616B
Verification
4. When the startup routine and background check are done, verify the sample syringe is
not leaking.
PN 4237616B 4.33-5
4.33-6 PN 4237616B
DRAINING BATH REPLACEMENTS 4
4.34 DRAINING BATH REPLACEMENTS
Purpose
Use this procedure to replace the O-rings on the draining baths.
B O-ring, FAA066A (one for each bath)
Preparation
2. From the Main Menu, select 4. DIAGNOSTICS tt DILUTER SYSTEMS tt 3. DRAIN BATHS tt
6. ALL BATHS.
4. Verify all the draining baths are empty.
O-ring Replacements
1. Locate the replacement O-rings.
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the drains
baths, you may become contaminated. To prevent possible biological contamination, you must wear
appropriate safety glasses, a lab coat, and gloves when servicing the drain baths.
ATTENTION: When removing a draining bath, the diffuser (which resembles a three-leg stool)
may pop out of the bath. If this happens, the diffuser must be retrieved, rinsed with distilled
water, and placed back inside the draining bath.
2. Grasp the bottom of the drain bath between two fingers and gently turn the bath to
loosen then remove it from its support (Figure 4.34-1).
Figure 4.34-1 Removing a Draining Bath
PN 4237616B 4.34-1
DRAINING BATH REPLACEMENTS
3. Remove and discard the old O-ring (Figure 4.34-2).

4. Clean then dry the top of the bath.
5. Make sure the diffuser is seated properly inside the draining bath. The circular part of the
diffuser must rest on the bottom of the draining bath with the tabs extended upward.
(Proper placement of diffuser resembles a three-legged stool turned upside down.)
6. Replace the O-ring (Figure 4.34-2)
Figure 4.34-2 Draining Bath O-ring Placement
7. Position the draining bath back on its support then gently rotate the bath to tighten it.
8. Repeat steps 2 through 7 at each draining bath (Figure 4.34-3).
Figure 4.34-3 Draining Bath O-Ring Locations
Verification
3. When the startup routine and background check are done, verify the draining baths are
not leaking.
4.34-2 PN 4237616B
O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and WBC/BASO Baths) 4
4.35 O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and WBC/BASO Baths)
Purpose
Use this procedure to replace the coaxial cable O-ring and the two counting head aperture
O-rings. This procedure instructs you to bypass the right side door interlock which allows
you to observe instrument operation with the right side door open. Avoid any unnecessary
contact with the sample probe. The probe is sharp and may contain biohazardous materials.
When the procedure is complete, make sure the interlock is reactivated to ensure the
customer is not injured by the sample probe or its movement.
B Allen wrenches, 3 mm, 2.5 mm, 2 mm
B Torque screwdriver
Note: 100 mN.m (14.2 Ozf.in) torque is required in this procedure.
B Hemostat or tweezers with a pointed end
B Small side cutters
B Protective cover for the bath electrode
Note: If you do not already have this special tool, you will need a micropipette tip and a
scalpel or sharp knife.
B White paper towel or cloth
B Cotton-tip applicator stick
B Distilled water
B Replacement parts
r Coaxial cable O-ring, PN - FAA046A (need 1 for each bath)
r Aperture O-ring, PN - GBG156A (need 2 for each bath)
Preparation
1. Locate the replacement coaxial cable O-rings (one needed for each bath) and the
aperture O-rings (two needed for each bath).
3. From the Service menu, bypass the right side door interlock:
a. Select 9. OTHERS tt 1. USER MODE.
b. Press the down arrow key to highlight the SERVICE option.
Note: This option bypasses the right side door interlock allowing you to observe
instrument operation with the right side door open.
c. Press the decimal point key then ESC to select the Service option.
d. Press ESC two more times to return to the Diagnostics menu.
4. From the Diagnostics menu, select 3. DILUTER SYSTEMS tt 3. DRAIN BATHS tt 6. ALL BATHS.
5. Open the right side door and verify all the baths are empty.
PN 4237616B 4.35-1
O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and WBC/BASO Baths)
7. Remove the left side panel and set it aside.
Removal
WARNING Risk of personal contamination. If you do not properly shield yourself while servicing the bath
assembly, you may become contaminated. To prevent possible biological contamination, you must wear
appropriate safety glasses, a lab coat, and gloves when servicing the bath assembly.
1. Before removing the baths assembly, position the sample probe to provide a rough
alignment guide when the baths assembly is replaced at the end of this procedure.
a. Manually push the sample probe housing over the rinse bath.
b. Gently push down on the top of the sample probe until the tip of the probe rests on
the inside rim of the rinse bath (Figures 4.35-1 and 4.35-2).
Figure 4.35-1 Sample Probe Position at the Rinse Bath Figure 4.35-2 Close-up of Probe Positioning
c. Without moving the probe position, manually push the sample probe as far forward
as possible to the front of the instrument. At the end of this procedure, the
positioning of this probe will provide a guide for aligning the baths assembly.
2. Place a piece of white paper or cloth under the baths assembly.
3. Record the tubing positions before dismantling the baths assembly.
4. Disconnect all bath tubings except the waste tubings.
5. Remove the three screws securing the baths assembly to the instrument panel
4.35-2 PN 4237616B
Figure 4.35-3 Baths Assembly Screw Locations
O-ring Replacements
Replacing the Coaxial Cable O-ring on the Bath Electrode

1. Loosen the two hex screws on the back of the RBC bath (Figure 4.35-4). Use a 2 mm
Allen wrench.
Figure 4.35-4 Electrode Screw Locations
2. Carefully remove the electrode from the bath.
CAUTION Risk of damage to the bath electrode. When removing the coaxial cable O-ring, you can
accidently cut the bath electrode. Make sure the O-ring is sufficiently clear of the electrode before cutting
the O-ring.
PN 4237616B 4.35-3
3. Remove the coaxial cable O-ring from the bath electrode as follows (Figure 4.35-5):
a. Use a small, pointed-tip tweezers to grasp the O-ring (Figure 4.35-5).
b. While gently lifting the O-ring to separate it from the electrode, carefully cut the
O-ring with a pair of small side cutters (Figure 4.35-5). Do not clip the electrode.
Figure 4.35-5 Removing the Coaxial Cable O-ring from the Bath Electrode
CAUTION Risk of damage to the bath electrode. When replacing the coaxial cable O-ring, you can
accidently break off the bath electrode. Use a plastic micropipette tip (cut to fit over the electrode) to protect
the bath electrode as you seat the O-ring.
4. If you have not already done so, use a plastic micropipette tip to make a protective cover
for the bath electrode (Figure 4.35-6).
r Use a scalpel or other sharp knife to cut the plastic micropipette tip.
r Shorten the tip until it fits securely over the bath electrode.
Figure 4.35-6 Making a Protective Cover from a Micropipette Tip
5. Position the new O-ring over the bath electrode as follows:

a. Place the coaxial cable O-ring on the protective cover (Figure 4.35-6).
b. Carefully position the protective cover over the bath electrode.
c. Push the O-ring from the plastic cover onto the bath electrode.
d. .Carefully remove the plastic cover from the electrode. Make sure the O-ring
remains on the bath electrode.
e. Verify the O-ring is properly seated.
4.35-4 PN 4237616B
6. Set the bath electrode aside. While the bath is disassembled, replace the aperture O-rings
in the counting head.
Replacing the Aperture O-rings in the Counting Head
IMPORTANT Risk of misleading results. The 50 µm RBC/PLT aperture and the 80 µm WBC/BASO aperture
are not interchangeable. Disassemble the RBC/PLT counting head, replace the aperture O-ring, and
reassemble the counting head before disassembling the WBC/BASO counting head.
ATTENTION: Be careful when handling an aperture, it is very small so it is easy to drop and easy
to lose. It is recommended that you work over a white surface so the aperture can be easily
seen if it is dropped.
1. If you have not already done so, place a piece of white paper or cloth under the baths
assembly.
2. Carefully dismantle the RBC/PLT counting head (Figure 4.35-7). Use a 2.5 mm Allen
key.
Note: In Figure 4.35-7, the RBC/PLT counting head is on the left and the WBC/BASO
counting head is on the right.
Figure 4.35-7 Bath Electrode Locations
3. When the counting head is separated from the bath, one of the O-rings may remain
inside the counting head. If this happens, remove and discard the O-ring and clean the
counting head.
PN 4237616B 4.35-5
4. Figure 4.35-8 shows the aperture and O-ring location on the bath when the counting
head is removed.
Figure 4.35-8 Location of the Aperture and Its Two O-Rings
CAUTION Risk of damage to the bath or aperture. Using a sharp instrument inside the bath may damage
the inside of the bath and the aperture. Never use a sharp instrument inside the bath.
5. Remove the aperture and the O-ring from the bath as follows:
a. Locate a cotton-tip applicator stick.
b. Hold the bath close to the surface of the white paper towel.
c. Insert the cotton tip of the applicator stick through the opening created when the
bath electrode was removed (at the rear of the baths assembly).
d. Gently push the aperture and O-ring onto the white paper surface.
6. Place the aperture in a small amount of distilled water to keep it moist and discard the
old O-ring(s).
7. Clean the bath and counting head as follows:
a. Pour diluted bleach on a soft, clean paper towel.
b. Clean the bath and the counting head using the wet paper towel.
c. Rinse the surfaces thoroughly with distilled water.
d. Dry the counting head and the exterior of the bath with a soft paper towel.
4.35-6 PN 4237616B
8. Install the new O-rings and replace old aperture as follows:
Note: When completed, the aperture should be sandwiched between the two aperture
O-rings.
a. Moisten the tip of your finger with distilled water then pick up one of the aperture
O-rings using your moistened finger.
b. Pass your finger (with the clinging O-ring) across the bath opening to seat the
O-ring in the groove.
CAUTION Risk of damage to the aperture. Do not handle the aperture using a hard instrument or tool.
Handle the aperture with your fingers. Clean the aperture gently between your two fingers.
c. Pick up the aperture with your finger tip then gently rub it between two fingers to
clean it. Rinse the aperture with distilled water.
d. Position the aperture on the tip of your moistened finger.
e. Pass your finger (with the clinging aperture) across the bath opening to seat the
aperture on the aperture O-ring already seated in the bath opening.
f. Moisten the second aperture O-ring and seat it inside the counting head.
CAUTION Risk of damage to the aperture. If too much pressure is applied as the bath components are
being reassembled, the aperture may break. To avoid applying too much pressure on the bath (and the
aperture), reconnect the counting head tubing before attaching the counting head back on the bath.
9. Reconnect the counting head tubings.

10. Carefully position the counting head back on the bath. Make sure the O-rings and
aperture are properly seated and do not become crimped as you position the counting
head on the bath.
11. Loosely install the two hex screws in the counting head.
CAUTION Risk of damage to the aperture. Overtightening the counting head hex screws can break the
aperture. Tighten these screws to a torque of 100mN.m (14.2 ozf.in) using a torque screwdriver with a
2.5 mm bit.
ATTENTION: Torque needed for the RBC (and WBC/BASO) bath hex screws is 100 mN.m
(14.2 ozf.in).
12. Tighten the two counting head hex screws. Use a 2.5 mm torque screwdriver to tighten
each screw to 100 nN.m (14.2 ozf.in).
13. Install the coaxial cable electrode that was set aside earlier:
a. Carefully insert the bath electrode inside the opening at the rear of the bath.
b. Loosely install the two hex screws.
c. Tighten the two hex screws. Use a 2 mm torque screwdriver to tighten each screw to
100 nN.m (14.2 ozf.in).
14. Repeat steps 2 through 13 on the WBC/BASO counting head. See Figure 4.35-7. (The
WBC/BASO counting head is the one on the right.)
PN 4237616B 4.35-7
Installation
1. Using your recorded tubing positions, reattach the bath tubings.
2. Being careful to not crimp any tubings, position the baths assembly against its support
panel.
3. Replace and loosely tighten the three screws removed earlier (Figure 4.35-3).
4. Make sure any liquid inside the bath compartment is wiped dry, especially any liquid on
the solenoid valves.
Align the Bath Assembly
CAUTION Risk of damage to the sample probe. If power is restored to the instrument and the baths
assembly is mounted too high, the sample probe may become bent when it hits the bath edges as it moves
from bath to bath. Do not restore instrument power until the baths assembly is aligned.
1. Manually push the sample probe housing to the outside edge of the rinse bath. Move the
baths assembly up or down as necessary until the tip rests gently on the outside rim of
the rinse bath.
2. While continuing to push the sample probe housing over the rinse bath to the inside
edge of the bath, adjust the bath assembly up or down as needed so that the sample
probe tip comes to rest gently on the inside rim of the rinse bath as shown in
(Figures 4.35-9 and 4.35-10).
.
Figure 4.35-9 Sample Probe Position at the Rinse Bath Figure 4.35-10 Close-up of Acceptable Probe Position
3. Gently tighten the center and left screws.

4. Without lifting the probe, gently push the probe housing towards the inside edge of the
last bath, the WBC/BASO bath. Reposition the baths assembly as needed to clear the edge
of each bath.
4.35-8 PN 4237616B
5. Verify the sample probe is positioned at the WBC/BASO bath as shown in
Figures 4.35-11 and 4.35-12.
Figure 4.35-11 Sample Probe Position, Right Side Figure 4.35-12 Close-up of Acceptable Probe Position
6. Gently tighten the right screw on the baths assembly.

7. Make sure all three screws are tight so that the baths assembly is secure on the
instrument’s frame.
8. Carefully move the sample probe housing over the outer edge of the rinse bath. The
probe should equally clear all baths. If not, loosen the nearest screw and reposition the
baths assembly until the distance between the tip of the probe and the top of each bath is
the same.
Verification
WARNING Risk of personal injury. The right side door interlock is bypassed which allows you to observe
instrument operation with the right side door open. Avoid any unnecessary contact with the sample probe.
The probe is sharp and may contain biohazardous materials. When the procedure is complete, make sure
the interlock is reactivated to ensure the Customer is not accidently injured by the sample probe or its
movement.
3. On the instrument keypad, press the STARTUP button to initiate the startup cycles and
background check.
4. As the startup routine and background check are being done,
a. Observe operation inside the right side compartment to verify there are no leaks.
Look at the entire compartment not just the baths assembly. If a tubing is connected
to a wrong port, the liquid in that tubing will be sent to an area that is not meant to
receive it.
b. Check for leaks inside the left compartment.
PN 4237616B 4.35-9
5. From the Service menu, select 9. OTHERS tt 1. USER MODE and reconnect the right side
door interlock.
a. Press the up arrow key to highlight the MANUAL option.
b. Press the decimal point key then ESC to select the Manual option.
c. Press ESC again to return to the Service menu.
CAUTION It is mandatory to perform the probe adjustment procedure after this adjustment.
6. Check the sample probe position inside each bath.

r Under Heading 4.6, SAMPLE PROBE CHECKS AND ADJUSTMENTS, do the Inside
Bath Position Check.
r Perform the adjustment if needed.
7. When the probe position inside each bath is correct, perform a system verification. See
Heading 5.3, SYSTEM VERIFICATION PROCEDURES.
4.35-10 PN 4237616B
OPTICAL BENCH PRELIMINARY ADJUSTMENTS 4
4.36 OPTICAL BENCH PRELIMINARY ADJUSTMENTS
Purpose
Use this procedure to make preliminary adjustments to the flow cell and optics in the optical
bench assembly. These adjustments may be necessary when the:
r Optical bench assembly is replaced.
r The flow cell is replaced.
r Transfer Time value is outside the acceptable range.
r Absorbance Channel value is outside the acceptable range.
r Optical lamp is replaced.
B Allen wrenches, 3 mm, 2 mm
Preparation

4. At the optical bench,
a. Remove the four hex screws shown in Figure 4.36-1.
b. Carefully remove the optical bench cover and set it aside.
Figure 4.36-1 Optical Bench Cover Screw Locations
PN 4237616B 4.36-1
OPTICAL BENCH PRELIMINARY ADJUSTMENTS
Verify the Flow Cell is Free of Bubbles

2. From the Main Menu, select 4. DIAGNOSTICS tt 3. DILUTER SYSTEM tt 2. RINSE tt
3. When the routine is complete, verify the flow cell is bubble-free. (An occasional air
bubble is acceptable.)
Course Adjustments
ATTENTION: Course adjustment of the flow cell positions the lens and flow cell system for final
alignment with the Absorbance Channel adjustment. Course adjustments may not be
necessary. If the Absorbance Channel value and the Transfer Time value are acceptable,
course adjustments are not needed.
Y-Axis Adjustment
CAUTION Never dismantle the lens assembly or even unlock it. The adjustment is factory made and cannot
be performed in the field.
CAUTION If you use a 3 mm Allen wrench to check the gap, be careful that you do not damage or scratch
the flow cell or lens assembly.
Use a 3 mm shim or Allen wrench to verify the gap between the lens assembly and the flow
cell is approximately 3 mm (Figure 4.36-2). The final adjustment will be close to this
position.
Figure 4.36-2 Checking the Lens to Flow Cell Gap
4.36-2 PN 4237616B
r If the gap is approximately 3 mm, go to the X-Axis Adjustment heading.
r If the gap is not close to 3 mm, use the front knob (Figure 4.36-3) to move the flow cell
forward or backwards (along the Y-axis) as needed.
Figure 4.36-3 Front Knob for Y-Axis Adjustment
X-Axis Adjustment
The lens assembly is fixed. During this course adjustment, the flow cell is repositioned as
needed along its X-axis so that the focal point of the light beam coming from the lens
assembly is properly positioned inside the flow cell.
1. From the rear of the instrument, look down the lens assembly and evaluate the position
of the flow cell in relation to the lens assembly. The flow cell must be centered with the
lens.
2. If the flow cell is located to the left or to the right of the lens, use the side screw
(Figure 4.36-4) to move the flow cell left or right (along the X-axis) so that the flow cell
is roughly centered with the lens.
Figure 4.36-4 Side Screw - X-Axis Adjustment
PN 4237616B 4.36-3
3. Place a piece of white paper between the flow cell and the reception diode as shown in
Figure 4.36-5.
Figure 4.36-5 How to Position the White Paper
4. From the rear of the instrument, look for a light spot on the white paper.
r If the lamp filament image on the white paper matches the ideal image seen in
Figure 4.36-6, go to step 5.
r If the image does not match the ideal projection seen in Figure 4.36-6, go to the
Lamp Alignment heading that follows before proceeding.
Figure 4.36-6 Ideal Lamp Filament Projection Image
4.36-4 PN 4237616B
5. Use the side screw (Figure 4.36-7) to position the flow cell in the center of the light spot.
6. Go to Heading 4.4, FLOW CELL CHECKS AND ADJUSTMENTS to ensure the diff
adjustment values are within acceptable limits.
Lamp Alignment
CAUTION A small 2 mm setscrew in the lamp housing allows adjustment of the optics lamp. This
adjustment is factory made and should not be changed unless absolutely nececessary.
The optics lamp can be aligned using a 2 mm setscrew in the lamp housing (Figure 4.36-8).
When this setscrew is loosened, the entire lamp, with its base, can be turned or moved up and
down. Make this adjustment only if the previous flow cell adjustments did not produce a
proper filament projection.
Figure 4.36-8 Lamp Adjustment Screw
1. Use a 2 mm Allen wrench to loosen the setscrew holding the lamp base in place
(Figure 4.36-8). Do not remove the setscrew.
PN 4237616B 4.36-5
2. While rotating the entire lamp with its base, move the lamp up or down as needed until
the lamp filament projection image matches the ideal projection image seen in
Figure 4.36-9.
Figure 4.36-9 Ideal Lamp Filament Projection Image
3. When the light spot matches the image seen in Figure 4.36-9, tighten the setscrew.
4. Use the side screw (Figure 4.36-10) to position the flow cell in the center of the light
spot.
5. Go to Heading 4.4, FLOW CELL CHECKS AND ADJUSTMENTS to ensure the diff
adjustment values are within acceptable limits.
4.36-6 PN 4237616B
FLOW CELL WBC BALANCE 4
4.37 FLOW CELL WBC BALANCE
Purpose
Use this procedure to balance the WBC count generated in the flow cell with the WBC count
generated and reported from the WBC/Baso bath. A comparison of these two counts is made
to ensure that there are no problems with either system. When they are too far apart, the
diff+/diff- flags are generated. These adjustments may be necessary when:
r the optical bench assembly is replaced.

r the flow cell is replaced.
r the flow cell aperture gain is adjusted.
r too many diff+ or diff- flags.
r WBC aperture and calibration have changed.
B Fresh blood samples
Procedure
1. From the Main Menu, select
4. Diagnostics tt 5. Service tt 8. Flowcell WBC Calibration tt 1. Autocalibration
The WBC/Flow Cell Balance screen is displayed (Figure 4.37-1).
Figure 4.37-1 WBC/Flow Cell Balance Screen
AUTOCALIBRATION 02/27/00 16:05

WBC FLOWCELL WBC
RUN AT LEAST 5 SAMPLES

AND PRESS ENTER TO CALIBRATE
STAT WBC FLOWCELL WBC
CURR 1.00 135
NEW ---- ----
MEAN ---- ----
2. Run a fresh normal blood sample at least 5 times. A mean will be established after 3 runs
and a new flow cell WBC count calibration factor will be calculated. The raw flow cell
WBC count is multiplied by this factor before being matched with the printed WBC
count from the WBC bath.
3. Press ENTER when the runs are complete. You will then be given the option of accepting
(ENTER) or rejecting (ESC) the new factor. There should be no flagging for the WBC
results. If there is, do not accept the new factor. Repeat the procedure, possibly with a
different fresh normal blood.
PN 4237616B 4.37-1
FLOW CELL WBC BALANCE
4.37-2 PN 4237616B
SETTING diff+/diff- THRESHOLDS 4
4.38 SETTING diff+/diff- THRESHOLDS
Purpose
Use this procedure to check or set the sensitivity for the diff+ and diff- flags. These flags
indicate a difference between the WBC count arrived at in the flow cell and the WBC count
derived in the bath.
B None
Procedure
1. From the Main Menu, select
4. Diagnostics tt 5. Service tt 8. FlowCell WBC Calibration tt 2. Cal Factors
The diff Flag Sensitivity screen is displayed (Figure 4.38-1):
Figure 4.38-1 diff Flag Sensitivity Screen
CAL FACTORS 02 / 27 / 00 16:05
%WBC BAL. 1 50.0

%WBC BAL. 2 20.0
%WBC BAL. 3 15.0
FLOW CELL WBC 0.95
WBC FLOW CELL BALANCE
7616104B
2. WBC FLOWCELL BALANCE LIMIT (%) XX.X lines set the sensitivity threshold for the
diff+/diff- flag, as a percentage. The default values are 50%, 20%, and 15% for BAL 1,
BAL 2, and BAL 3 respectively.
For example, for WBC BAL 2, the diff+/diff- flag will be triggered when enabled, if the
(calibration factor corrected)WBC count from the flow cell is more than 20% different
from the (calibration factor corrected) WBC BAL 2 count at the WBC/Baso bath.
The FLOWCELL WBC XXX line indicates the flow cell WBC calibration factor that was
determined by Heading 4.37, FLOW CELL WBC BALANCE. The flow cell WBC
calibration factor can be altered or manually entered on this screen.
The last line, WBC FLOWCELL BALANCE allows the diff+/diff- flagging to be disabled by
clearing the enable/disable button.
PN 4237616B 4.38-1
SETTING diff+/diff- THRESHOLDS
4.38-2 PN 4237616B
MAIN CARD REPLACEMENT AND SOFTWARE TRANSFER 4
4.39 MAIN CARD REPLACEMENT AND SOFTWARE TRANSFER
Purpose
Use this procedure to replace the Main Card and transfer the software EPROMs to the new
card. The new card is then checked to ensure the settings are correct.
If a software upgrade is also being performed, refer to the appropriate service memo.
B Allen wrench
B IC puller, PN 5450537
B External digital pressure/vacuum gauge (referred to as vacuum meter)
B Voltmeter
Preliminary Setup
1. Set the instrument to the Manual Startup mode.
From the Main Menu, select
4. Diagnostics tt 5. Service tt 9. Others tt 1. User Mode then Manual Startup.
2. Print out the system settings before removing the Main card.
From the Main Menu, select
5. Setup tt 6. Others tt 8. Print System Setup.
Note: After the new card is installed, the new settings are compared to these settings and
adjustments made as necessary.
Removing Main Card

1. Turn off the instrument and unplug the power cord from the wall outlet.
2. Remove the left side panel (Heading 4.2).
3. Starting at the top left corner of the Main card (Figure 4.39-1), remove the cable plugs
from the connections shown in Table 4.39-1.
4. Using a 3-mm Allen wrench, remove the nine screws from the Main card in accordance
with the sequence shown below.
ATTENTION: At last position (9), do not allow the screw to drop into shield. Rather, hold
the wrench against the screw while removing the card. Turn the card down to allow the
screw to drop through the hole in shield.
8 9 1
7 6 2
5 4 3
PN 4237616B 4.39-1
MAIN CARD REPLACEMENT AND SOFTWARE TRANSFER
5. Remove the Main card.
Figure 4.39-1 Main Card - Connections and EPROMs

J35 J25 J22 J23 J24 J26
LMNE LMNE GR GB
OD CIS RBC WBC
J20
J1
J16
J17
J36
J18
J14
J15
U43
J31 J30 J33 J27 J29 U42 J37 J2 J34 J9 J5 J4 7616120B
4.39-2 PN 4237616B
Table 4.39-1 Main Card - Plug/Jack Connections
Number of Wires
Cable Plug Color (type) Label (on board)
P35 red, blk 2 J35 (3-pin connector)
LMNE OD (P25) blk (coax) LMNE OD
LMNE CIS (P22) LMNE CIS
GR RBC (P23) GR RBC
GB WBC (P24) GB WBC
P26 wht, blu, yel, brn 4 J26
P36 wht 4 J36
P4 gry (10-wire ribbon) J4
P5 wht 4 J5 (10-pin connector)
P9 gry (ribbon) J9 (40-pin connector)
P2 gry (ribbon) J2 (26-pin connector)
P37* (pwr supply) red 12 J37
P29 red, grn, blk, wht 4 J29
P30 red, grn, blk, wht 4 J30
P33† red (3), wht (2), blk 6 J33
P31† red (2), wht (2) 4 J31
P15, P14 gry (ribbon) J15, J14
P18, P17, P16 J18, J17, J16
(14-pin connectors)
P1 blk ribbon J1 (26-pin connector)
P20 gry ribbon J20 (52-pin connector)
* P37 plug holes are offset to the top. DO NOT use excessive force when installing plug.
† Plug label shows thermistor values in ohms.
Transferring EPROMs to Replacement Card

Note: Software EPROMs are not provided with the replacement card.
CAUTION DO NOT pry up EPROMs with screwdriver. Prying up the EPROMs can damage the lands on the
card. Remove the EPROMs with an IC puller.
1. Using the IC puller, remove the EPROMs U42 (right) and U43 (left) from the old Main
card. See Figure 4.39-1 for location. Set the puller over the EPROM and pull straight out.
2. Check the EPROM labels for proper identification, software version and addresses (U42
is labeled "odd" and U43 labeled "even").
3. Insert U42 (right) and U43 (left) into their plug-ins on the card, NOTCH UP.
PN 4237616B 4.39-3
Installing Main Card

1. Using a 3-mm Allen wrench, install the nine screws to secure the Main card to the
instrument in accordance with the sequence shown below.
ATTENTION: At position 1, start threading the screw through the shield into the card first,
then hold the wrench against the screw and mount the card on the instrument. Complete
threading the screw but leave it loose for aligning other screws.
2 1 9
3 4 8 Note: Secure the ground strap at position 2.
5 6 7
2. Install all connections listed in Table 4.39-1.
CAUTION If, following the Beckman Coulter logo the instrument begins a rinse cycle, immediately TURN
OFF the instrument. Damage can occur to the probe if the instrument was not set for Manual Startup mode.
3. Connect power cord to ac wall outlet and turn on the instrument. The Beckman Coulter
logo, along with the revision level of the software (x.xx) is displayed on the screen in
approximately 7 seconds. Continue to check the instrument settings as outlined in
"Verifying System Configuration" and "Verifying Main Card Settings" below.
a. If only a single line appears on the screen, turn off the instrument and check the
positions of the EPROMs (U43 and U42); they may be reversed.
b. If the instrument begins a rinse cycle:
1) Turn off the instrument.
2) Open the right side door and turn on the instrument. One or both of the
folowing messages is displayed:
SYSTEM ERROR RUN SYSTEM RESET CYCLE
BATH ENCLOSURE DOOR OPEN
ESC
3) Press to access the Main Menu and select
4. Diagnostics tt 5. Service tt 9. Others tt 1. User Mode then Manual Startup.
4. Power Off, close the right side door and power On again. The instrument should not
attempt to do a rinse cycle.
4.39-4 PN 4237616B
Verifying System Configuration
Use this procedure when replacing a Main card and the configuration of the replacement card
is unknown.
1. Reset values to default. From the Main Menu, select

4. Diagnostics tt 5. Service tt 9. Others tt 4. Setting to Default Values.
The instrument prints the current values and then resets to the defaults. Enter the
settings as listed in Table 4.39-2 and compare them to the original system setup (printed
in step 2 of "Preliminary Setup").
:
Table 4.39-2 AC•T 5diff Menu Paths - System Settings
Parameter From Main Menu...

Date Format 5. Setup tt 1. Date/Time tt 2. Date Format
Time Format 5. Setup tt 1. Date/Time tt 1.Time Format
Units 5. Setup tt 2. Units
Language 5. Setup tt 6. Others tt 5.Language.
If American English, set "USA" to selected or (enable).
Manual Startup 4. Diagnostics tt 5. Service tt 9. Others tt 1. User Mode then Manual Startup
Service 4. Diagnostics tt 5. Service tt 9. Others tt 1. User Mode
Identification Mode 5. Setup tt 6. Others tt 2. Identification Mode
Bar Code with Checksum 5. Setup tt 6. Others tt 2. Identification Mode
Display DIFF # 5. Setup tt 6. Others tt 2. Identification Mode
Enable ATL, IMM, PCT, 5. Setup tt 6. Others tt 2. Identification Mode
PDW
Sequence Number 5. Setup tt 6. Others tt 2. Identification Mode.
Resets to "1".
Identification Number 5. Setup tt 6. Others tt 2. Identification Mode.
Resets to "1".
Reset Time 5. Setup tt 6. Others tt 2. Identification Mode
Operators 5. Setup tt 6. Others tt 1. Calibration tt 2. Define Operators
Calibration Factors 2. Calibration tt 2. Cal Factors
CV% Limits 5. Setup tt 6. Others tt 1. Calibration tt 1. CV% Limits
Flow Cell WBC Calibration 4. Diagnostics tt 5. Service tt 8. Flowcell WBC Calibrationn tt 2. Cal Factors
%WBC BA1 50.0
%WBC BA2 20.0
%WBC BA3 15.0
FLOW CELL WBC X.XX
WBC FLOW CELL BALANCE (enable)
Daily Workload 3. Reagents tt 2. Daily Workload
PN 4237616B 4.39-5
Table 4.39-2 AC•T 5diff Menu Paths - System Settings (Continued)
Parameter From Main Menu...

Cycle Counts 5. Setup tt 6. Others tt 7. Cycle Counts
Resets to "0".
Autoclean Frequency 5. Setup tt 6. Others tt 3. Autoclean Frequency
Burn In 4. Diagnostics tt 5. Service tt 7. Burn-In tt 1. Burn-In Cycles
Heating Coil Thermistor 4. Diagnostics tt 5. Service tt 3. Heating Systems tt 1. Heating Coil tt 1. Adjustment.
Value Press ENTER for calculation after updating the value.
Heating Coil Reference 4. Diagnostics tt 5. Service tt 3. Heating Systems tt 1. Heating Coil tt 2. Reference.
Temperature Adjust reference temperature setting.
Bath Enclosure 4. Diagnostics tt 5. Service tt 3. Heating Systems tt 2. Bath Enclosure tt 1. Adjustment
Thermistor Value Press ENTER for calculation after updating the value.
Bath Enclosure Reference 4. Diagnostics tt 5. Service tt 3. Heating Systems tt 2. Bath Enclosure tt 2. Reference
Temperature Adjust reference temperature setting.
Dilution 4. Diagnostics tt 5. Service tt 1. Dilution
Mixing 4. Diagnostics tt 5. Service tt 4. Mixing
Vacuum 4. Diagnostics tt 5. Service tt 6. Vacuum Check tt 1. Counting
Pulse Adjustment 4. Diagnostics tt 5. Service tt 2. Measurement tt 6. Pulse Adjustment
Reagent Volumes 5. Setup tt 6. Others tt 6. Reagent Volumes
Reagent Lot Numbers 3. Reagents tt 1. Level Change then select Change All.
Enter the lot numbers from the old system settings. After the last number is entered, the
instrument primes all the reagents.
Note: If new or full reagent containers are not placed in the system, the cycle count will not
represent the actual volume of reagent in the bottles.
Printer Configuration 5. Setup tt 5. Printer tt 1. Printer Configuration.
In addition, Rev 1.03 or later has Histograms Thresholds as an option.
Institutional Header 5. Setup tt 5. Printer tt 2. Institutional Header
Sending Options 5. Setup tt 4. Host Setup tt 3. Sending Options
Host Setup Configuration 5. Setup tt 4. Host Setup tt 1. Host Setup Configuration
Format 5. Setup tt 4. Host Setup tt 2. Sending Configuration
Variable Format Setup 5. Setup tt 4. Host Setup tt 4. Variable Format Setup
Patient Ranges, Action Ranges and Thresholds

If the instrument was using the default values for Patient Ranges, Action Ranges and Thresholds
(on all 9 ranges), then the software retains these values. If the values were not set to default,
then re-enter the values from the old system setup.
Note: Rev 1.03 Software defaults to "Simple Flagging" and no "Threshold Flags." To set the
DIFF Plot and Histogram Flags to On, from the Main Menu, select
5. Setup tt 5. Printer tt 1. Printer Configuration then enable DIFF Plot and Histogram Flags.
4.39-6 PN 4237616B
Verifying Main Card Settings
Use this procedure to verify the Main card settings after the replacement of the card.
1. Compare the settings listed in Table 4.39-3 to the readings taken from the Main card and
adjust as required. Use Figure 4.39-2 to locate the potentiometer and corresponding test
point.
:
Table 4.39-3 AcT 5diff - Main Card Settings
Function (Heading) Adjustment Target Range Test Point

Motor Current Adjustment Draining Syringe R149 4.0 V ±0.05 V TP5
(4.13) Counting Syringe R150 4.0 V ±0.05 V TP6
Diluter Syringe R151 4.0 V ±0.05 V TP7
Injector Syringe R152 3.0 V ±0.05 V TP8
Horizontial Carriage R154 3.0 V ±0.05 V TP10
Sample Syringe R155 2.0 V ±0.05 V TP11
Probe Carriage R156 4.5 V ±0.05 V TP12
Threshold Adjustments BASO R157 300 mV ±5 mV TP14
(4.14) RBC R158 300 mV ±5 mV TP13
PLT R159 300 mV ±5 mV TP2
FLOW CIS R160 650 mV ±5 mV TP3
FLOW OD R161 350 mV ±5 mV TP4
Drain Sensor Adjustment Drain Sensor R287 4.5 V (empty) ±0.3 V TP52
(4.11) <1.0 V (full) (under P30)
DIFF Bath Drain Sensor Diff Bath Drain R286 4.5 V (empty) ±0.3 V TP48
Adjustment (4.12) Sensor <1.0 V (full) (under P29)
Vacuum Checks and Counting Syringe Step value 6.5 inches Hg stable test box attached
Adjustments (4.17) to bottom port
on side of
syringe
Vacuum Checks and Draining Syringe Non-adjustable 7.7 inches Hg stable test box attached
Adjustments (4.17) to top port on
side of syringe
HGB Blank Adjustment (4.7) R248 4.7 V ±0.3 V screen
Aperture Current Check Non-adjustable 60 V J22, J23, J24

(4.8)
RBC/PLT Gain Adjustment RBC Gain R133 Channel 78 screen
(4.9) PLT Gain R135 Channel 112 screen
WBC/BASO Gain R134 Channel 102 screen
Adjustment (4.10)
PN 4237616B 4.39-7
Table 4.39-3 AcT 5diff - Main Card Settings
Function (Heading) Adjustment Target Range Test Point

DIFF Lamp Voltage R11 6.0v 5.50 - 6.50 V screen
Adjustment (4.4)
Transfer Time (4.4) shim 200 150 - 250 screen
Resistive Channel R136 50 45 - 55 screen
Adjustment (4.4) Final Gain Using R136 good scatterplot
Fresh Whole Blood scatterplot
distribution
Absorbance Channel R148 >170 adjust to max screen
Adjustment (4.4) 170 - 190
Final Gain Using R148 good scatterplot
Fresh Whole Blood scatterplot
distribution
2. Use a fresh normal whole-blood specimen to check instrument reproducibility.

a. From the Main Menu, select 2. CALIBRATION tt 4. REPRODUCIBILITY.
b. Run eleven samples and then delete the first one for a ten-shot reproducibility.
c. Verify the reproducibility results are within acceptable limits. See Table 4.39-4.
.
Table 4.39-4 Whole-Blood Reproducibility CV Limits for 20 Cycles
Parameter %CV Test Level

WBC < 2.0% at 10.0 x 103/µL
RBC < 2.0% at 5.00 x 106/µL
Hgb < 1.0% at 15.0 g/dL
Hct < 2.0% at 45.0%
MCV < 1.0% at 90.0 fL
Plt < 5.0% at 300 x 103/µL
3. Run controls and confirm results fall within expected ranges.
4.39-8 PN 4237616B
Figure 4.39-2 Main Card - Potentiometers /Test Points
R151/ R150/ R149/ R161/ R148 R160/ R136 R135 R159/ R158/ R248 R133
TP7 TP6 TP5 TP4 TP3 TP2 TP13
LMNE LMNE GR GB
OD CIS RBC WBC
R152/
TP8
R134
R157/
TP14
R154/
TP10
R155/
TP11
R156/
TP12
R287/ R286/
TP52 TP48 7616121B
PN 4237616B 4.39-9
4.39-10 PN 4237616B
CONTENTS
5 MAINTENANCE PROCEDURES, 5.1-1
5.1 RECOMMENDED MAINTENANCE SCHEDULE, 5.1-1
5.2 MAINTENANCE WORKLIST, 5.2-1

Purpose, 5.2-1
Additional Tools or Supplies Needed for 1-Year Maintenance, 5.2-1
Additional Tools or Supplies Needed for the Every 2-Years Maintenance, 5.2-1
User Mode, 5.2-1
Worklist Instructions, 5.2-2
Overview, 5.2-2
Maintenance Category Identifier, 5.2-2
Basic Instructions, 5.2-2
Preparation, 5.2-3
Reagent Syringes Assembly, 5.2-4
Procedure, 5.2-4
5diff Syringe Assembly, 5.2-5
Procedure, 5.2-5
Count Syringe Assembly, 5.2-5
Procedure, 5.2-5
Replace the Optical Bench Lamp, 5.2-6
Procedure, 5.2-6
Replace the Flow Cell Coaxial Cable, 5.2-7
Procedure, 5.2-7
Preparation, 5.2-7
Sample Probe and Rinse Block Assembly, 5.2-8
Procedure, 5.2-8
Sample Syringe Assembly, 5.2-8
Procedure, 5.2-8
Waste Syringe Assembly, 5.2-9
Procedure, 5.2-9
Diluent Reservoir, 5.2-9
Procedure, 5.2-9
Preparation, 5.2-10
Draining Baths, 5.2-11
Procedure, 5.2-11
PN 4237616B 5-i
CONTENTS
Counting Heads, 5.2-11

Procedure, 5.2-11
Clean the Bath Enclosure, 5.2-11
Wrap Up, 5.2-12
5.3 SYSTEM VERIFICATION PROCEDURES, 5.3-1

Purpose, 5.3-1
Preparation, 5.3-1
Startup, 5.3-1
Reproducibility, 5.3-1
Calibration, 5.3-2
Autocalibration, 5.3-2
Run Calibration, 5.3-2
Calibration Passed, 5.3-2
Step-By-Step Cycle Check, 5.3-3
Preparation, 5.3-3
Instrument At Rest, 5.3-3
Sample Preparation (Making the Dilutions), 5.3-3
Count and Measurement of the WBC Group, 5.3-5
RBC/PLT Group Count, 5.3-6
Permanent Rinse Flow (PRF), 5.3-6
Filling of Diluent Reservoir, 5.3-6
Completing the Cycle, 5.3-6
Wrap Up, 5.3-6
TABLES
5.1-1 Maintenance Schedule, 5.1-1
5-ii PN 4237616B
5MAINTENANCE PROCEDURES 5
5.1 RECOMMENDED MAINTENANCE SCHEDULE
Table 5.1-1 lists the maintenance procedures for the AC•T 5diff hematology analyzer and
when they should be performed. Maintenance is based on the number of cycles; therefore, the
frequency a maintenance procedure needs to be done is driven by the customer workload.
The maintenance schedule in Table 5.1-1 is the customer workload translated into a time
schedule.
Table 5.1-1 Maintenance Schedule
Workload (Cycles Per Day) <30 30 to 120 >120

PMI Kit
Maintenance Frequency 1Y 2Y 6M 1Y 18M 2Y 6M 1Y
Replace rinse block O-ring and washer X X X X X X X X 6 months
Replace reagent syringe O-rings and washers X X X X X X X X Maintenance Kit,
PN - XEA485AS
Replace waste syringe O-ring and washer X X X X X X X X
Replace count syringe O-ring and washer X X X X X X X X
Clean the bath enclosure X X X X X X X X
Replace sample syringe O-rings X X X X X X 1 year
Replace 5diff syringe O-rings X X X X X X Maintenance Kit,
PN - XEA486AS
Replace draining chamber O-rings X X X X X X
Replace optical bench lamp X X X X X X
Replace flow cell coaxial cable X X X X X X
Replace diluent reservoir O-ring and washer X X X X X X
Replace counting head coax and aperture O-rings X X X X X X
Replace sample probe and rinse block X X X Every 2 years
Maintenance Kit,
Replace reagent syringe pistons X X X
PN - XEA581AS
Replace waste syringe piston X X X
Replace count syringe piston X X X
PN 4237616B 5.1-1
RECOMMENDED MAINTENANCE SCHEDULE
5.1-2 PN 4237616B
MAINTENANCE WORKLIST 5
5.2 MAINTENANCE WORKLIST
Purpose
Use this procedure as a guide when the 6-months, 1-year, or every 2-years maintenance is
needed. 6-months maintenance mainly consists of replacing O-rings and washers. The 1-year
maintenance is more extensive and includes all the procedures that would typically be done
during the 6-months maintenance. The every 2-years maintenance is the most extensive
because it also includes all the 6-months and 1-year maintenance procedures.
A worklist is provided to guide you through the various procedures and checks that need to
be done to complete a scheduled maintenance. Make a copy of this worklist before starting
the maintenance procedures and use it as a checklist to help you keep track of your progress.
You may also use this worklist as a record if you sign and date it.
B 6 months maintenance kit, PN - XEA485AS
B Allen wrench, 2.5 mm and 3.0 mm
B Torque screwdriver, 2.5 mm hex-ball and T10 torx
Note: 100 mN.m (14.2 ozf.in) and 400 mN.m (56.8 ozf.in) torques are required.
B Cutting pliers
B Distilled water
B Lint-free tissues
Additional Tools or Supplies Needed for 1-Year Maintenance

B 1 year maintenance kit, PN - XEA486AS
B Small Phillips-head screwdriver
B Hemostat or tweezers with a pointed end
B Small side cutters
B White paper towel or cloth
B Cotton-tip applicator swab
Additional Tools or Supplies Needed for the Every 2-Years Maintenance

B Every 2 years maintenance kit, PN - XEA581AS
User Mode
While performing these maintenance procedures, you are instructed to set the User mode to
Service. In this mode, the right side door interlock is bypassed. When the instrument is
operating with the right side door open, it is important to avoid any unnecessary contact with
the sample probe. The probe is sharp and may contain biohazard materials. When the service
call is complete, make sure the interlock is reactivated to ensure the Customer is not
accidently injured by the sample probe or its movement.
PN 4237616B 5.2-1
MAINTENANCE WORKLIST
Worklist Instructions
Overview
The maintenance worklist is a composite guide for the 6-months, 1-year, and every 2-years
maintenance procedures. After providing you with the steps required to prepare the
instrument for maintenance, the worklist directs you to the individual service and repair
procedures needed to complete maintenance in the left compartment. When these
replacements are complete, an interim verification check is done to ensure there is no leakage
before moving to the right compartment. After performing replacements inside the right
compartment, another verification is done to again ensure there is no leakage. When all the
required maintenance procedures are completed, an overall system verification is done to
ensure proper operation.
Maintenance Category Identifier

Since this worklist is a composite of the three possible maintenance schedules, a block
containing the maintenance category appears before each procedure:
[6 months / 1 year / 2 years] indicates a procedure that must be completed each time
maintenance is done on an AC•T 5diff hematology analyzer.
[1 year / 2 years] indicates a procedure that is completed only when the 1 year or every
2 years maintenance is done. When doing a 6 months maintenance, this procedure is not
required.
[6 months] appears only once to direct you to perform the System Verification Procedures
when you are doing a 6 months maintenance.
If you are doing a 6 months maintenance, follow the instructions in the rows marked
[6 months / 1 year / 2 years] or [6 months]. If you are doing a 1 year or every 2 years maintenance,
follow the instructions in the rows marked [6 months / 1 year / 2 years] or [1 year / 2 years].
Basic Instructions
Preparation instructions for performing the required maintenance are provided at the
beginning of the worklist. These instructions supersede the preparation instructions in the
individual service and repair procedures. As a result, when you are directed to a particular
service and repair procedure, you are usually directed to begin at the Removal heading and
further directed as to what subsequent headings need to be completed. In most cases, you will
not complete the Verification in the individual procedures. When all the required
maintenance procedures are completed, you will be directed to do an overall system
verification to ensure the instrument is operating properly before you close out the service
call. It is important for you understand that by carefully reading and following the directions
associated with each procedure you will avoid doing unnecessary work.
Before starting a maintenance call, make a copy of the complete worklist that begins on the
next page. Since this worklist consolidates all the individual procedures, it is important to
progress through the procedures in their designated order. Use this worklist as a guide to
ensure all needed maintenance procedures are completed. As you finish each task, place a
checkmark (c) next to the completion statement to keep track of your progress as you work
through the various procedures.
5.2-2 PN 4237616B
Copy this worklist and use it as a guide to ensure all needed maintenance procedures are
completed. In the electronic version of this manual, a file (worklist.pdf) is provided for
printing. As you finish each task, place a checkmark (c) next to the completion statement to
keep track of your progress as you work through the various procedures.
Replacements in the Left Side Compartment

[6 months / 1 year / 2 years]
Preparation
1. Use the maintenance kit part listings to ensure no parts are missing from the kit.
r If performing a 6-months maintenance, use Table 8.1-10.
r If performing the 1-year maintenance, use Table 8.1-10 and 8.1-11.
r If performing every 2-years maintenance, use Table 8.1-10, 8.1-11, and 8.1-12.
Note: If you press the decimal point too long, the dot may disappear. The dot
indicates the right side door interlock is bypassed.
c. Press ESC to return to the Others menu.
5. From the Others menu, select 3. PARK SYRINGES. When the park syringes prompt
appears, press ENTER.
6. When the park syringes routine is complete, turn the instrument off.
b. Remove the six hex screws securing the rear access panel.
Note: Use a 3 mm hex key to remove any hex screws securing an instrument
cover. For details, see Heading 4.2.
M O D
N O .
A S S Y
1 0 0 -2 4 0 5 0 /6 0
C A U T IO N :
O R B A C K .
c. Remove the rear access panel and set it aside.
PN 4237616B 5.2-3
8. Remove the four hex screws securing the left side panel to the instrument frame and
set the door aside.

a. Turn the two captive knobs counterclockwise to release the Main card door.
These knobs are located to the right of the Main card.
[1 year / 2 years]
10. Remove the top cover:
a. In the left side compartment, remove the hex screw in the front upper corner.
b. Open the right side door and remove the hex screw in the front upper corner.
c. At the rear of the instrument, remove the three hex screws securing the top cover
to the instrument frame.
d. Carefully remove the top cover and set it aside.
_________ Preparation complete.

Reagent Syringes Assembly
Replacement Parts
From the 6 months maintenance kit: From the every 2 years maintenance kit:
B Washer and silicone O-ring, B Reagent syringe piston,
PN - XDA622A (4 sets) PN - GBC030A (need 4)
B Silicone O-ring for the Hgb Lyse B Hgb Lyse reagent syringe piston,
reagent syringe piston, PN - FAA065A PN - GBC031A
B Silicone grease, PN - XEA019A
Procedure
Under Heading 4.24, REAGENT SYRINGES ASSEMBLY REPLACEMENTS, complete the
following sections:
_______ Removal
_______ O-Ring, Washer, and Piston Replacement

r If performing the 6 months or 1 year maintenance, replace the O-rings and
washers only. Do not replace the pistons.
r If performing the every 2 years maintenance, replace all pistons, O-rings,
and washers.
_______ Installation
_________ All reagent syringes assembly replacements are complete.
5.2-4 PN 4237616B
[1 year / 2 years]
5diff Syringe Assembly
Replacement Parts
From the 6 month maintenance kit: From the 1 year maintenance kit:
B Silicone grease, PN - XEA019A B Silicone O-ring, PN - FAA040A
B Fluorocarbon O-rings, PN - FAA067A
(need 4)
Procedure
Under Heading 4.29, 5diff SYRINGE ASSEMBLY REPLACEMENTS, complete the
following sections:
_______ Removal
_______ O-ring Replacements

Note: Replace five O-rings, one silicone and four fluorocarbon.
_________ All five O-rings are replaced.

Count Syringe Assembly
Replacement Parts
B Washer and O-ring, PN - XDA621A B Count syringe piston, PN - GBG052A
Procedure
Under Heading 4.25, COUNT SYRINGE COMPONENT REPLACEMENTS, complete the
following sections:
_______ Removal
_______ O-ring, Washer, and Piston Replacement

r If performing the 6 months or 1 year maintenance, replace the O-rings and
washers only. Do not replace the pistons.
r If performing the every 2 years maintenance, replace all pistons, O-rings,
and washers.
_________ All count syringe replacements are complete.
PN 4237616B 5.2-5

Interim Verification Check
3. Press the STARTUP button on the instrument keypad.
4. When the Startup routine and background check are done,
r If performing the 6 months maintenance, verify the reagents syringe assembly
and the count syringe assembly are not leaking.
r If performing the 1 year or every two years maintenance, verify the reagents
syringe assembly, the 5diff syringe assembly, and the count syringe assembly are
not leaking.
5. Open the right side door and check the right compartment for leaks. A tubing
connected on the wrong port inside the left compartment may cause leaking inside the
right compartment.
6. If no leakage is seen, prime the reagents.
From the Main Menu, select 3. REAGENTS tt 3. PRIME tt 6. ALL REAGENTS.
7. When the prime cycles are done,
a. Check the reagents syringe assembly, the 5diff syringe assembly (if applicable),
and the count syringe assembly for leaks.
b. Check the right side compartment for leaks.
8. If no leaks are detected,
a. Turn the instrument off.
b. Disconnect the power cord.
c. Do the electronic replacements that follow.
_________ Syringe assemblies inside the left compartment are not leaking.
[1 year / 2 years]
Replace the Optical Bench Lamp
Replacement Part
From the 1 year maintenance kit:
B Optical bench lamp, PN - DAJ007A
Procedure
Under Heading 4.31, OPTICAL BENCH LAMP REPLACEMENT, complete the following
sections:
_______ Removal
_______ Lamp Replacement

_________ Optical bench lamp is replaced.
5.2-6 PN 4237616B
[1 year / 2 years]
Replace the Flow Cell Coaxial Cable
Replacement Part
B DIFF flow cell coaxial cable, PN - XBA399A
Procedure
Under Heading 4.30, FLOW CELL COAXIAL CABLE REPLACEMENT, complete the
following sections:
_______ Removal
_______ Replacement
_______ Under Heading 4.4, do the Flow Cell Checks.

Note: If a value is outside acceptable limits, do the appropriate adjustment.
_________ Flow cell coaxial cable is replaced and the flow cell checks are within acceptable limits.
Replacements in the Right Side Compartment

Preparation
1. From the Service menu, select 9. OTHERS tt 3. PARK SYRINGES. When the park syringes
prompt appears, press ENTER.
2. When the park syringes routine is complete, turn the instrument off.
3. Disconnect the power cord.
5. Manually push down on the waste syringe piston until it reaches its lowest position.
PN 4237616B 5.2-7

Sample Probe and Rinse Block Assembly
Replacement Parts
From the 6 month maintenance kit: From the 2 year maintenance kit:
B O-ring, fluorocarbon, PN - FAA053A B Sample probe, PN - XDA619AS
B Silicone grease, PN - XEA019A B Sample probe guide, PN - GBG091A
Procedure
In the right side compartment, manually push the sample probe housing towards the rear
of the instrument then under Heading 4.26, SAMPLE PROBE AND RINSE BLOCK
ASSEMBLY COMPONENT REPLACEMENTS, complete the following sections:
_______ Removal
_______ Rinse Block Assembly Component Replacement

r If performing the 6 months or 1 year maintenance, replace the O-ring only.
r If performing the every 2 years maintenance, replace the sample probe, the
probe guide, and its O-ring.
_________ All needed components are replaced.
[1 year / 2 years]
Sample Syringe Assembly
Replacement Part
B Fluorocarbon O-ring, PN - FAA064A (need 2)
Procedure
Under Heading 4.33, SAMPLE SYRINGE ASSEMBLY REPLACEMENTS, complete the
following sections:
_______ Removal
_________ O-rings replaced.
5.2-8 PN 4237616B
Waste Syringe Assembly
Replacement Parts
B Washer and O-ring, PN - XDA621A B Waste syringe piston, PN - GBG052A
Procedure
Under Heading 4.27, WASTE SYRINGE COMPONENT REPLACEMENTS, complete the
following sections:
_______ Removal
_______ O-ring, Washer, and Piston Replacement

r If performing the 6 months or 1 year maintenance, replace the O-ring and
washer only. Do not replace the piston.
r If performing the every 2 years maintenance, replace the syringe piston,
O-ring, and washer.
_________ All waste syringe replacements are complete.
[1 year / 2 years]
Diluent Reservoir
Replacement Parts
B O-ring and washer, PN - XEA286AS
Procedure
Under Heading 4.32, DILUENT RESERVOIR REPLACEMENTS, complete the following
sections:
_______ Removal
_______ O-ring and Washer Replacement
_________ O-ring and washer replaced.
PN 4237616B 5.2-9

Interim Verification Check
instrument operation with the right side door open. Avoid any unnecessary contact with the sample
probe. The probe is sharp and may contain biohazardous materials.
3. Press the STARTUP button on the instrument keypad.

4. When the Startup routine and background check are done,
r If performing the 6 months maintenance, verify the waste syringe is not leaking.
r If performing the 1 year or every two years maintenance, verify the waste syringe,
the diluent reservoir, and the sample syringe are not leaking.
5. Check the left side compartment for leaks. A tubing connected on the wrong port
inside the right compartment may cause leaking inside the left side compartment.
a. When the traverse service position prompt appears, press ENTER.
b. Verify the sample probe rinse block is not leaking.
c. Press ESC twice to return to the Diagnostics menu.
_________ Assemblies inside the right and left compartments are not leaking.
[1 year / 2 years]
Preparation
1. From the Diagnostics menu, select 3. DILUTER SYSTEMS tt 3. DRAIN BATHS tt
6. ALL BATHS.
2. Verify all baths (including the draining baths and rinse chamber) are empty.
4. Disconnect the power cord.
5. Do the draining bath and counting head replacements that follow.
5.2-10 PN 4237616B
[1 year / 2 years]
Draining Baths
Replacement Parts
B O-ring, PN - FAA066A (one for each bath)
Procedure
Under Heading 4.34, DRAINING BATH REPLACEMENTS, complete:

_________ O-rings are replaced.
[1 year / 2 years]
Counting Heads
Replacement Parts
B Coaxial cable O-ring, PN - FAA046A (need 1 for each bath)
B Aperture O-ring, PN - GBG156A (need 2 for each bath)
Procedure
Under Heading 4.35, O-RING REPLACEMENTS IN THE COUNTING BATHS (RBC and
WBC/BASO Baths), complete the following sections:
_______ Removal
_______ Align the Bath Assembly
_______ Verification which also includes the SYSTEM VERIFICATION PROCEDURES.

_________ Coaxial cable and aperture O-rings are replaced. None of the baths are leaking.
_________ All system verification checks are acceptable.

Clean the Bath Enclosure
Under Heading 4.28, CLEANING THE BATH ENCLOSURE, complete the procedure as
written.
_________ Bath enclosure cleaned as directed.
PN 4237616B 5.2-11

Wrap Up
[6 months]
instrument operation with the right side door open. Avoid any unnecessary contact with the sample
probe. The probe is sharp and may contain biohazardous materials.
Perform a system verification as instructed under Heading 5.3, SYSTEM VERIFICATION

PROCEDURES.
_________ All system verification checks are acceptable.
[1 year / 2 years]
1. Replace the top cover:
a. Carefully position the top cover on the instrument.
b. In the left side compartment, replace the hex screw in the front upper corner.
c. Open the right side door and replace the hex screw in the front upper corner.
d. At the rear of the instrument, replace the three hex screws that secure the top
cover to the instrument frame.
2. Replace the rear access panel using the six hex screws removed earlier.
a. Lift the white plastic catch to release the Main card.
b. Close the Main card door and turn the two captive knobs clockwise to secure it.
WARNING Risk of personal injury. The right side door interlock is bypassed allowing operation with the
right side door open. The probe is sharp and may contain biohazardous materials. When all the
maintenance procedures are complete, make sure the interlock is reactivated to ensure the Customer is
not accidently injured by the sample probe or its movement.

b. Press the decimal point or DEL key. Make sure the dot no longer appears inside
the SERVICE box. The right side door interlock is reactivated.
Note: If you press the decimal point too long, the dot may reappear. The dot
indicates the right side door interlock is bypassed.
c. Press ESC as many times as necessary to return to the Main Menu.
_________ All panels and covers are replaced. The right side door interlock is reactivated.
5.2-12 PN 4237616B
_________ 6 months maintenance procedures are completed.
_________ 1 year maintenance procedures are completed.
_________ Every two years maintenance procedures are completed.
Date of Completion _________________________________________________________
Institution _________________________________________________________________
Completed by ______________________________________________________________
PN 4237616B 5.2-13
5.2-14 PN 4237616B
SYSTEM VERIFICATION PROCEDURES 5
5.3 SYSTEM VERIFICATION PROCEDURES
Purpose
Use this procedure at the end of each service call to verify the AC•T 5diff hematology analyzer
is operating correctly.
B Fresh, normal whole- blood specimens
B Calibration blood specimens
Preparation
the instrument, you may become injured or contaminated. To prevent possible injury or biological
contamination, you must wear appropriate safety glasses, a lab coat, and gloves when servicing the
instrument.
IMPORTANT Risk of misleading results. This procedure must be performed on a clean instrument. If it is
suspected that the instrument is not perfectly clean, perform the PREPARATION TO SHIP THE
INSTRUMENT procedure under Heading 4.3.
Startup
Run a Startup cycle and background check.
Reproducibility
Use one fresh, normal whole-blood specimen to check instrument reproducibility.
1. From the Main Menu, select 2. CALIBRATION tt 4. REPRODUCIBILITY.

2. Do a 20 sample reproducibility study in the CBC mode of operation. Verify the results
from these runs are not flagged.
3. Verify the reproducibility results are within acceptable limits. See Table A.1-6.
4. Run a fresh, normal whole-blood specimen in the CBC/DIFF to verify normal specimens
are not flagging in the CBC/DIFF mode.
PN 4237616B 5.3-1
SYSTEM VERIFICATION PROCEDURES
Calibration
Autocalibration
1. From the Main Menu, select 2. CALIBRATION tt 1. AUTOCALIBRATION.
2. At the Autocalibration screen, change the lot number, expiration date, or target values as
necessary.
Run Calibration
1. Prepare the calibrator according to the specific instructions (temperature, mixing, and so
forth).
2. Open the vial and submerge the sample probe inside the vial.
3. Press the aspirate switch located behind the probe.
4. When the cycle LED stops flashing, remove the vial and replace the cap on the calibrator.
5. When the analysis cycle ends, the result is displayed.
IMPORTANT The calibration of the AC•T 5diff hematology analyzer can be performed on 3 to 11 analyses.
To obtain the best calibration possible, it is recommended to run the calibration blood a minimum of five
times. The autocalculation module performs statistics on these results to obtain the best calibration factors.
6. Remix the calibrator and repeat steps 2 through 5 until at least three, but no more than
11 calibrator samples are analyzed. It is recommended that you run the calibrator at least
five times to achieve the best calibration.
Note: After three runs, the instrument calculates calibration statistics.
Calibration Passed
Calibration passes if the statistics are within the acceptable limits:
r Coefficient of variation (CV) is within the limits setup as described.
r The percentage difference between the target and the mean value is less than 20.
a. Press Enter to save the new calibration factors.
b. Press Enter again to print the new calibration factors.
2. Check that the calibration factors are within the acceptable ranges listed in Table A.1-7.
5.3-2 PN 4237616B
Step-By-Step Cycle Check
Use this step-by-step description of the cycle as a guide to help you verify proper instrument
operation at the end of a service call. This check covers the four principal phases of a cycle:
1. Sample preparation (making the dilutions)

2. Count and measurement of the WBC group
3. RBC/PLT group count
4. Filling the diluent reservoir
A special cycle is required when no other cycle is started during RBC/PLT count or during the
filling of the diluent reservoir: rinsing of the RBC bath.
All phases are described in this procedure. Use Figures A.3-1 and A.3-2 If you need help
locating an assembly.
Preparation
1. Remove the four hex screws securing the left side panel to the instrument frame and set
the door aside.
2. If you have not already done so, change the User mode to Service. This bypasses the right
side door interlock so you can cycle with the right side door open.
From the Service menu, select 9. OTHERS tt 1. USER MODE. For details, see User Mode
under Heading 4.1.
Instrument At Rest
When the AC•T 5diff analyzer is at rest (power is ON but the instrument is not cycling),
verify:
1. The sample probe is in its home position and the green LED is glowing.
2. In the right compartment, verify all the baths (except the rinse chamber) are filled with
diluent and the piston inside the waste syringe is up.
3. Inside the left compartment, verify the pistons inside the count syringe, 5diff syringe,
and reagent syringes assemblies are down.
Note: It will be necessary to cycle a whole-blood specimen several times to see the following
actions.
Sample Preparation (Making the Dilutions)
PN 4237616B 5.3-3
Verify sample probe movement and dilution preparation:
B Press the aspirate switch. All the baths are drained (waste syringe).
B 53 µL whole-blood sample is aspirated (sample syringe).
B Sample probe moves over the rinse chamber (sample probe, probe motor, traverse
motor).
B 3 µL sample aliquot at the tip of the sample probe is discarded into the rinse chamber as
the exterior of the sample probe is rinsed (sample syringe, diluent reagent syringe).
B Sample probe moves from the rinse chamber to the first dilution (DIL1/HGB) bath
(sample probe motor, traverse motor).
B 10 µL of whole-blood partitioned for making the first dilution is delivered to the
DIL1/HGB bath using a tangential flow of 1.7 mL of diluent (sample syringe, diluent
reagent syringe). Mixing bubbles enter the bath. This is the primary 1:170 dilution (or
first dilution).
B Sample probe moves from the first dilution (DIL1/HGB) bath to the WBC/BASO bath
(sample probe motor, traverse motor).
B 10 µL of whole-blood partitioned for making the WBC/BASO dilution is delivered to the
WBC/BASO bath using a tangential flow of 2.0 mL of WBC Lyse (sample syringe, WBC
Lyse reagent syringe). Mixing bubbles enter the bath. This is a 1:200 dilution.
B Sample probe moves from the WBC/BASO bath to the DIFF bath (sample probe motor,
traverse motor).
B 25 µL of whole-blood partitioned for making the DIFF dilution is delivered to the DIFF
bath using a tangential flow of 1.0 mL of Fix reagent (sample syringe, Fix reagent
syringe). Mixing bubbles enter the bath.
B Sample probe moves from the DIFF bath to the rinse chamber (sample probe motor,
traverse motor).
B 5 µL of residual blood is expelled during the double rinsing (interior and exterior) of the
sample probe (sample syringe, diluent reagent syringe).
B After 12 seconds of incubation, the dilution inside the DIFF bath is completed by adding
another 1.0 mL of diluent which stops the cytochemical reaction (5diff syringe).
Making the DIFF dilution detailed:
r Sample probe is positioned opposite the entry port for the Fix reagent.
r Solenoid valve 8 is energized which also sets up the reagent syringes assembly
movement to dispense 1.0 mL of Fix reagent.
r 25 µL of whole-blood sample is simultaneous delivered to the DIFF bath.
r Solenoid valve 8 de-energizes.
r DIFF dilution incubates for 12 seconds.
r Solenoid valves 1 and 3 are energized which sets up the 5diff syringe to dispense
1.0 mL of diluent is added to the DIFF bath to stop the cytochemical reaction.
r Solenoid valves 1 and 3 are de-energized.
5.3-4 PN 4237616B
Making the second dilution for RBC and PLT analysis:
B Sample probe moves from the rinse chamber the first dilution (DIL1/HGB) bath (sample
probe motor, traverse motor).
B 42.5 µL of the primary 1:170 dilution is aspirated (sample syringe).
B Sample probe exterior is rinsed (in the first dilution bath) with 0.4 mL of diluent
(diluent reagent syringe).
B Sample probe moves from the first dilution (DIL1/HGB) bath to the RBC bath (sample
probe motor, traverse motor).
B 42.5 µL of the first 1:170 dilution is delivered to the RBC bath using a tangential flow of
2.0 mL of diluent (sample syringe, diluent reagent syringe). The tangential flow of
reagent mixes the 1:170 dilution sample and the diluent to make a uniform suspension
of cells. Mixing bubbles also enter the bath. This is the final RBC/Plt dilution.
B An additional 0.5 mL of diluent is dispensed through the probe at the end of the second
dilution.
Hemoglobin measurement:
B Once the 42.5 µL of dilution is removed, the exterior of the sample probe is rinsed with
0.4 mL of diluent. 0.4 mL of Hgb Lyse reagent is then dispensed into the first dilution
(DIL1/HGB) bath. Mixing bubbles enter the bath. The Hgb Lyse reagent destroys the red
blood cells and converts a substantial proportion of the hemoglobin to a stable pigment
so a hemoglobin value can be determined on the final 1:250 dilution.
Count and Measurement of the WBC Group

DiffPlot acquisition and WBC count:
B Loading of the vacuum injection circuit of the optical bench (waste syringe, energized
solenoid valves 4 and 5).
B Start flow in the flow cell (approximately 2 seconds).
B Injection into the flow cell is 15 seconds but acquisition of data for the DiffPlot and WBC
count occurs only 12 seconds.
Simultaneously, the WBC and BASO counts are gathered.

B Rinse of the counting head (the dilution pushed into position, solenoid valves 9, 10, 21,
23 and 13 are energized).
B Start the vacuum and first part of count (duration of 6 seconds, solenoid valve 23
energized).
B Rinsing of counting head and adjustment of the vacuum count.
B Second part of count (duration of 6 seconds).
After this first series of measurements:

B Measurement of the hemoglobin.
B Rinsing of the WBC/BASO bath with a mixture of 1.06 mL of Rinse reagent and 1.44 mL
of diluent.
B Rinsing of the WBC/BASO counting head.
PN 4237616B 5.3-5
RBC/PLT Group Count

B Rinsing the RBC/PLT counting head.
B Start the vacuum for the RBC/PLT counting head.
B First series of counts (duration of 5 seconds, solenoid valve 14 energized).
B Rinsing of the RBC/PLT counting head and adjustment of the vacuum.
B Second series of counts (duration of 5 seconds).
Permanent Rinse Flow (PRF)

B The WBC/BASO and the RBC/PLT counting heads are in series during the RBC/PLT
count. The count flow from the WBC bath circulates in the RBC/PLT counting head and
limits the possibility of the circulation of the RBC behind the count orifice.
B The PRS functions similar to the autocleaning function in that it acts like a cleaning
solution being drawn through the WBC/BASO aperture during the entire duration of the
RBC and PLT counts.
Filling of Diluent Reservoir

B At the end of the RBC/PLT count, the RBC count vacuum is used for filling the diluent
reservoir. Solenoid valves 15 and 25 are energized.
B To improve the filling speed, the syringe is initialized for a few seconds then drawn again
to complete the filling.
B A float located in the tank controls the level of liquid.
Completing the Cycle

B To maximize mechanical resources, rinsing the RBC bath is normally programmed for
the beginning of the succeeding cycle (which can be started during the RBC and PLT
count).
B If no other cycle is started during the RBC/PLT count or during the filling of the diluent
reservoir, a special rinsing sequence is initiated:
r The sample signal light changes to red and the instrument is unavailable for a few
seconds.
r This sequence also involves draining of the RBC bath followed with a refilling with
the diluent.
Wrap Up
1. Replace the left side panel. Secure the panel using the four hex screws removed earlier.
3. Make sure the right side door interlock is reactivated. See User Mode under Heading 4.1,
as needed.
4. Turn the instrument off for 5 seconds then turn the instrument back on. An automatic
Startup routine and background check is performed.
5. When the Startup and background checks are complete, verify the results are acceptable.
5.3-6 PN 4237616B
CONTENTS
6 SCHEMATICS, 6.1-1
6.1 ENGINEERING SCHEMATIC DRAWINGS, 6.1-1

Engineering Schematics, 6.1-1
Additional Pneumatic/Hydraulic Information, 6.1-1
Tubings and Connectors, 6.1-1
Additional Interconnect Information, 6.1-1
6.2 PNEUMATIC / HYDRAULIC SCHEMATIC, 6.2-1

Layout, 6.2-1
Color Coding, 6.2-1
Tubing Designations, 6.2-1
Solenoid Valves, 6.2-1
6.3 PNEUMATIC/HYDRAULIC TUBINGS AND CONNECTIONS, 6.3-1

Tubings and Connectors List, 6.3-1
6.4 INTERCONNECT DIAGRAM, 6.4-1
6.5 MOTORS AND CABLES, 6.5-1
ILLUSTRATIONS
6.5-1 Horizontal Traverse Motor (PN - XBA391A), 6.5-1
6.5-2 Upper Fan (PN - XBA393A), 6.5-2
6.5-3 Horizontal Traverse Sensor (IR Sensor, PN - XBA394AS), 6.5-3
6.5-4 Bath Drain and DIFF Transfer Sensor (IR Sensor, PN - XBA395AS), 6.5-4
6.5-5 Vertical Traverse Sensor (IR Sensor, PN - XBA396AS), 6.5-5
6.5-6 IR Sensor (PN - XBA397AS), 6.5-6
6.5-7 RBC/WBC Coaxial Cable (PN - XBA398A), 6.5-7
6.5-8 DIFF Flow Cell Coaxial Cable (PN - XBA399AS), 6.5-8
6.5-9 Bar-Code Reader Cable (PN - XBA402AS), 6.5-9
6.5-10 Diluent Level Sensor (PN - XDA605AS), 6.5-10
TABLES
6.3-1 Instrument Tubing and Connectors , 6.3-1
6.3-2 Circuit Connections , 6.3-3
PN 4237616B 6-i
CONTENTS
6-ii PN 4237616B
6SCHEMATICS 6
6.1 ENGINEERING SCHEMATIC DRAWINGS
This chapter contains a variety of troubleshooting aids including fold-out engineering
schematics and interconnect diagrams. Details concerning the instrument’s
pneumatic/hydraulic tubing and connections are also provided.
Because the engineering schematics are insserted into the document and are not assigned
page numbers or figure numbers, they are not included in the table of contents or the index.
In the text, they are referenced by their name.
Engineering Schematics
This chapter contains two schematics:
r Pneumatic/hydraulic schematic
r Interconnect schematic
The schematic revision levels were current on the date this revision of the manual was
released. They will be updated again to the latest revision level whenever this manual is
revised.
Additional Pneumatic/Hydraulic Information
Tubings and Connectors

In addition to the pneumatic/hydraulic schematic, a listing of the tubings and connectors
used in the instrument are detailed in Table 6.3-1. This table also includes part numbers.
Pneumatic/Hydraulic Circuit Connections

Table 6.3-2 details the connections within each circuit including the diameter and length of
tubing needed to make the connection. Tubing part numbers can be located on Table 6.3-1.
Additional Interconnect Information

Some components on the interconnect engineering schematic are detailed in diagrams. These
interconnect diagrams are located under Heading 6.5.
PN 4237616B 6.1-1
SCHEMATICS
ENGINEERING SCHEMATIC DRAWINGS
6.1-2 PN 4237616B
SCHEMATICS
PNEUMATIC / HYDRAULIC SCHEMATIC 6
6.2 PNEUMATIC / HYDRAULIC SCHEMATIC
Layout
The pneumatic/hydraulic schematic for the AC•T 5diff analyzer is laid out like the
instrument. On the left side of the schematic are the assemblies you find in the left side
compartment, including the count syringe, the 5diff syringe, the reagent syringes assembly,
the flow cell, and the associated solenoid valves. The right side of the schematic shows the
assemblies located behind the right side door, including the baths assembly, the diluent
reservoir, the waste syringe, the sample syringe, and the sample probe and rinse block
assembly.
Color Coding
The pneumatic/hydraulic schematic is color coded to correlate with the color used on the
reagent labels. The arrangement of reagents bottles correlates with their arrangement inside
the reagent compartment.
r AC•T 5diff Rinse lines are blue.
r AC•T 5diff Fix reagent lines are yellow.
r AC•T 5diff Hgb Lyse reagent lines are orange.
r AC•T 5diff WBC Lyse reagent lines are green.
r The AC•T 5diff Diluent reagent lines are magenta.
r All waste lines are color-coded red.
Tubing Designations
All tubing on this instrument is considered critical length. Notice there are two numbers
associated with each tubing. The first number is the internal diameter of the tubing in
millimeters; the second, is the length of the tubing in millimeters. For example, locate the
count syringe. Notice the tubing attached to the bottom fitting also attaches to port 1 of
solenoid valve 16. The number 2.05 125 is displayed above that line. This number indicates
the tubing has a 2.05 mm diameter and is 125 mm long. A box containing a translation of
inches to millimeter is located In the upper right corner. Although you may find this helpful,
it does not contain all tubing measurements. The numbers will be close but, as in our
example, not exact. Since the tubing is critical length, you may want to have a metric ruler
available.
Most of the tubing used on this instrument is Tygon tubing. The legend in the upper right
corner lists a second tubing, Cristal tubing which is used in limited quantities. In the lower
right corner of the schematic, notice the waste container and the diluent container both use
Cristal tubing. The Cristal tubing is designated by the letter C. The tubing used for the waste
line has a larger diameter (4 mm) than the tubing used for the diluent (3 mm). These tubings
are not interchangeable.
Solenoid Valves
Two types of solenoid valves are used on this instrument, a normally-closed two-way valve
and a three-way valve. Inside the box to the right of the reagent bottle, LV designates the
electronic valve or solenoid valve. Valve numbers may also be preceded by a V, an S, and an SL
designation.
PN 4237616B 6.2-1
SCHEMATICS
PNEUMATIC / HYDRAULIC SCHEMATIC
6.2-2 PN 4237616B
TRANSLATION Inch to mm (i.d.)
SAMPLE SYRINGE
+ DRAIN
+ BUBBLING
+ DIFF TRANSFER
+ PROBE WIPE
1.85 10
WASTE
SYRINGE
(RESERVOIR FILLING)
VENT
NOTCHED TUBING
DILUENT RESERVOIR
HGB LYSE
WBC LYSE
DILUENT
RINSE
FIX
WBC NOTCHED
RINSE DIL 1 DIFF RBC TUBING
BASO
VENT HGB 2.05 15
NOTCHED TUBING
2.05 50
5diff SYRINGE REAGENT SYRINGES
2.54 140
1.52 350
LV1: Differential diluent - Selects flow cell sheath 2 or DIFF bath

WBC LYSE
HGB LYSE
LV2: Differential diluent - Selects flow cell sheath 1 or sheath 2

LV4: Flow cell sample supply - Opens pathway from the DIFF bath to the flow cell
RINSE
FIX
LV5: Flow cell sample injector - Opens waste path for sample injector syringe
LV10: Diluent output control - Routes diluent to probe rinse block or heating coil
LV12: Rinse output control - Selects rinse to probe rinse block or WBC/BASO bath SLEEVING
LV14: RBC/PLT count valve - Opens vacuum count line for RBC bath
LV20: Waste syringe vent - Opens waste vent (through the rinse chamber) EXTERNAL TUBING
LV21: Sweep flow diluent - Routes diluent to heating coil or sweep flow
LV22: Diluent bath select - Routes diluent (via heating coil) to Hgb or RBC bath
LV23: WBC/BASO count vacuum - Routes vacuum direct or through RBC/PLT counting head
LV24: Flow cell drain: Opens path from flow cell output to DIFF bath for drain
SCHEMATIC DIAGRAM ACT 5diff: PNEUMATIC / HYDRAULIC
7616069B
SCHEMATICS
PNEUMATIC/HYDRAULIC TUBINGS AND CONNECTIONS 6
6.3 PNEUMATIC/HYDRAULIC TUBINGS AND CONNECTIONS
Tubings and Connectors List
The tubings and connectors used in the AC•T 5diff hematology analyzer are detailed in
Table 6.3-1. This table also includes part numbers.
Table 6.3-1 Instrument Tubing and Connectors
Designation Part Number Diameter Length Quantity

Y-connector EAB021A 3 1
Y-connector EAB026A 2.5 1
T410 connector EAB033A 1.6 1
T220 connector EAB035A 2.3 8
0.040" Tygon® tubing EAE005A 1.02 10 1
0.040" Tygon tubing EAE005A 1.02 95 1
PN 4237616B 6.3-1
SCHEMATICS
PNEUMATIC/HYDRAULIC TUBINGS AND CONNECTIONS
Table 6.3-1 Instrument Tubing and Connectors (Continued)

6.3-2 PN 4237616B
SCHEMATICS
Table 6.3-1 Instrument Tubing and Connectors (Continued)

Sleeving GAL098A 27
Tube shielding XBA403A 1
Pneumatic/Hydraulic Circuit Connections

In this table, each circuit connection has several descriptions. As you review the table, you
will notice that many of the descriptions have a name or number, followed by a line, followed
by a number. LV13_1 is a typical example of this format. LV13_1 is read as port 1 of valve 13
or valve 13, port 1.
Table 6.3-2 Circuit Connections
Circuit From F.S. Diameter Length T.S. To

AIR Atmosphere 2.05 140 LV13_2
LV13_1 1.52 70 Count syringe_1
Atmosphere 2.05 100 LV15_1
LV15_3 1.52 400 Diluent reservoir_2
Rinse_2 chamber 2.05 300 LV20_2
(Atmosphere)
LV20_1 1.52 90 Waste syringe_1
PN 4237616B 6.3-3
SCHEMATICS
Table 6.3-2 Circuit Connections (Continued)

DILUENT REAGENT Diluent Input 2.05 170 LV25_2
LV25_1 S 2.54 140 S Diluent reservoir_4
2.05 65 Diluent reservoir_1
Diluent reservoir_1 2.05 350 LV3_1
LV3_3 S 2.05 120 Injector syringe_2
LV3_2 S 1.52 50 S LV2_3
LV2_1 S 1.52 50 S LV1_3
LV1_1 S 1.52 200 S Isolator_1
Isolator_2 S 1.52 100 T1_1
T1_2 1.14 35 DIFF flow cell_4
T1_2 xba403a DIFF flow cell_4
T1_3 1.14 35 DIFF flow cell_2
T1_3 xba403a DIFF flow cell_2
LV1_2 1.52 250 T4_3
LV2_2 1.02 175 Injector syringe_4
Injector syringe_1 1.02 205 DIFF flow cell_5
DIFF flow cell_7 1.02 10 (Cap)
2.05 65 Diluent reservoir_3
Diluent reservoir_3 2.05 480 LV9_1
LV9_3 2.05 120 Reagent syringe_4
LV9_2 1.52 50 LV10_3
LV10_1 1.52 15 T3_2
T3_1 1.52 640 LV18_3
LV18_1 1.52 330 Probe rinse block_1
Probe rinse block_2 1.52 300 LV17_1
LV17_2 1.52 820 T5_2
LV18_2 1.02 110 Sample syringe_2
LV10_2 1.52 380 LV21_3
LV21_1 1.52 50
LV22_1 1.52 200 S Reagent heater_7
Reagent heater_8 S 1.52 120 RBC bath_1
Reagent heater_2 S 1.52 120 DIL1/HGB bath_1
LV21_2 1.52 220 WBC/BASO bath_4
6.3-4 PN 4237616B
SCHEMATICS

RINSE REAGENT Rinse reagent bottle 2.05 400 LV7_1
LV7_2 1.52 230 LV12_3
LV12_1 1.52 350 WBC/BASO bath_1
LV12_2 1.52 280 T3_3
FIX REAGENT Fix reagent bottle 2.05 400 LV8_1
Reagent heater_4 S 1.52 120 DIFF bath_3
WBC LYSE REAGENT WBC Lyse reagent bottle 2.05 400 LV11_1
Reagent heater_9 S 1.52 60 S Reagent heater_11
Reagent heater_12 S 1.52 120 WBC/BASO bath_2
HGB LYSE REAGENT Hgb Lyse reagent bottle 1.52 350 LV6_1
LV6_2 1.52 480 DIL1/HGB bath_2
SAMPLING Probe_1 1.02 205 Sample syringe_1
DIFF COUNTING DIFF bath_4 1.30 20 M1_2 photocell
M1_1 photocell 1.30 320 LV4_1
LV4_2 1.02 130 T2_2
T2_3 1.02 240 Injector syringe_5
Injector syringe_3 1.02 95 LV5_1
LV5_2 2.05 420 T6_2
T2_1 S 1.85 10 DIFF flow cell_6
0.19 4 DIFF flow cell_6
DIFF flow cell_output 1.52 20 E1_1 anode fitting
E1_2 anode fitting 1.52 70 Isolator_1
Isolator_2 1.52 80 E2_1 ground fitting
E2_2 ground fitting 1.52 335 LV24_2
LV24_1 1.52 120 T4_2
T4_1 1.52 160 S Reagent heater_5
Reagent heater_6 S 1.52 120 DIFF bath_2
PN 4237616B 6.3-5
SCHEMATICS

WBC/RBC COUNTING WBC/BASO bath_3 1.52 200 LV23_3
LV23_1 1.52 200 RBC bath_3
RBC bath_2 1.52 430 LV14_2
WASTE Rinse chamber_3 2.05 100 LV27_2
LV27_1 2.05 30 T7_3
DIL1/HGB bath_3 2.05 70 LV28_2
LV28_1 2.05 20 T7_2
T7_1 2.05 20 T8_3
DIFF bath_5 2.05 70 LV29_2
LV29_1 2.05 20 T8_2
T8_1 2.05 20 T9_3
RBC bath_4 2.05 60 LV30_2
LV30_1 2.05 20 T9_2
T9_1 2.05 20 T10_2
WBC/BASO bath_5 2.05 55 LV31_2
LV31_1 2.05 20 T10_1
T10_3 2.05 30 T6_3
T6_1 2.05 20 T5_3
T5_1 2.05 50 M2_1 photocell
M2_2 photocell 2.05 15 Isolator_1
Isolator_2 2.05 280 LV26_1
LV26_3 S 2.54 150 S Waste syringe_2
LV26_2 S 2.54 130 T11_3
Count syringe_5 2.05 125 LV16_1
LV16_2 S 2.54 340 T11_2
T11_1 2.54 20 Waste output
6.3-6 PN 4237616B
SCHEMATICS
INTERCONNECT DIAGRAM 6
6.4 INTERCONNECT DIAGRAM
The fold-out interconnect diagram for the AC•T 5diff analyzer is divided into five sections
using a dash/dot line. These five sections include:
r Power Supply module in the upper left corner.
r Measurement components on the left, under the Power Supply module.
r Main card in the center.
r Input/output interfaces to the right of the Main card.
r System inputs and outputs.
This interconnect diagram is a helpful troubleshooting tool, especially when troubleshooting
an electronic problem.
PN 4237616B 6.4-1
SCHEMATICS
INTERCONNECT DIAGRAM
6.4-2 PN 4237616B
SCHEMATICS
MOTORS AND CABLES 6
6.5 MOTORS AND CABLES
Figure 6.5-1 Horizontal Traverse Motor (PN - XBA391A)
PN 4237616B 6.5-1
SCHEMATICS
MOTORS AND CABLES
Figure 6.5-2 Upper Fan (PN - XBA393A)
6.5-2 PN 4237616B
SCHEMATICS
MOTORS AND CABLES 6
Figure 6.5-3 Horizontal Traverse Sensor (IR Sensor, PN - XBA394AS)
PN 4237616B 6.5-3
SCHEMATICS
MOTORS AND CABLES
Figure 6.5-4 Bath Drain and DIFF Transfer Sensor (IR Sensor, PN - XBA395AS)
6.5-4 PN 4237616B
SCHEMATICS
MOTORS AND CABLES 6
Figure 6.5-5 Vertical Traverse Sensor (IR Sensor, PN - XBA396AS)
PN 4237616B 6.5-5
SCHEMATICS
MOTORS AND CABLES
Figure 6.5-6 IR Sensor (PN - XBA397AS)
6.5-6 PN 4237616B
SCHEMATICS
MOTORS AND CABLES 6
Figure 6.5-7 RBC/WBC Coaxial Cable (PN - XBA398A)
PN 4237616B 6.5-7
SCHEMATICS
MOTORS AND CABLES
Figure 6.5-8 DIFF Flow Cell Coaxial Cable (PN - XBA399AS)
6.5-8 PN 4237616B
SCHEMATICS
MOTORS AND CABLES 6
Figure 6.5-9 Bar-Code Reader Cable (PN - XBA402AS)
PN 4237616B 6.5-9
SCHEMATICS
MOTORS AND CABLES
Figure 6.5-10 Diluent Level Sensor (PN - XDA605AS)
6.5-10 PN 4237616B
CONTENTS
7 TROUBLESHOOTING, 7.1-1
7.1 ERROR MESSAGES, 7.1-1
7.2 CHECKING THE MOTORS, 7.2-1

Purpose, 7.2-1
Preparation, 7.2-2
To Check All Motors, 7.2-2
To Only Check Motors in the Right Side Compartment, 7.2-2
To Only Check Motors in the Left Side Compartment, 7.2-2
Motors Check, 7.2-3
Wrap Up, 7.2-3
7.3 CHECKING THE VALVES, 7.3-1

Purpose, 7.3-1
Preparation, 7.3-1
To Only Check Valves in the Left Side Compartment, 7.3-1
To Only Check Valves in the Right Side Compartment, 7.3-1
Valves Check, 7.3-2
Wrap Up, 7.3-3
ILLUSTRATIONS
7.3-1 Valve 1 through 16 Locations, 7.3-2
7.3-2 Valves 17 and 18 Location, 7.3-2
7.3-3 Valves 20 through 31 Locations, 7.3-2
TABLES
7.1-1 Error Messages, 7.1-1
PN 4237616B 7-i
CONTENTS
7-ii PN 4237616B
7TROUBLESHOOTING 7
7.1 ERROR MESSAGES
Table 7.1-1 Error Messages
Displayed Message Problem Corrective Action

AT LEAST 3 TAGGED RESULTS At least 3 results are required for Run at least three results for
REQUIRED calibration calculations and less than 3 calculation results to be generated.
have been run.
BATH ENCLOSURE DOOR OPENED If a cycle is attempted while the right Close door to continue.
side door is open, this message is
generated.
DATA NOT SAVED, VALUE OUT OF The value typed in is not an acceptable Re-enter the data
RANGE value. It may be out of an expected
range or an incorrect data type.
DRAIN TIMEOUT Draining problems. 1. Run a System Reset cycle.
2. If the problem persists, call your
Beckman Coulter Representative.
ENTER AN IDENTIFICATION An ID is required to run an analysis in Enter the specimen ID.
the Manual ID mode.
INCORRECT DATE ENTRY The value entered is not a valid date. Enter a valid date.
INCORRECT TIME ENTRY The time entered is not a valid time. Enter a valid time.
NO ACK CHARACTER RECEIVED ON There is a problem with the Check that the protocol that has been
RS232 communication or handshaking to the set up in the host transmission screen
host computer. matches the protocol expected by the
host computer.
NO DILUENT, CHECK LEVEL The diluent reservoir is not able to fill. Check the diluent level and replace
diluent if necessary.
NO ENQ CHARACTER RECEIVED ON There is a problem with the Check that the protocol that has been
RS232 communication or handshaking to the setup in the host transmission screen
host computer.
PRINTER ERROR, CHECK PAPER An error indication has been sent from 1. Ensure there is paper in the Printer.
the Printer to the instrument, usually a 2. Check the Printer user’s manual for
paper out message. other Printer errors.
REAGENT LOW LEVEL [REAGENT The calculated reagent level for the 1. Check the reagent level and replace
NAME] specified reagent indicates no reagent. the reagent if necessary.
2. Update the reagent level.
REAGENTS LOW LEVEL This message is given at the end of Monitor the reagent levels, or change
startup if there is not enough reagent the reagents and update the levels.
left to complete the daily workload that
has been set up.
PN 4237616B 7.1-1
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-1 Error Messages (Continued)
Displayed Message Problem Corrective Action

SYSTEM ERROR, RUN SYSTEM During a cycle, a system error of the 1. Check the specified motor to make
RESET CYCLE following type has caused the system sure it is not jammed, and there are
to stop: no signs of physical damage to
r A motor has not returned to its motor mechanism or sensor.
home sensor when expected. 2. Check the bath compartment for
r A drain problem has been detected leaks, plugs, loose tubing. Ensure
at one of the two drain sensors. that there is sufficient reagent and
that the system is primed.
r The right side door has been
opened during a cycle, losing 3. Ensure that there is no physical
temperature control at the baths. damage to the microswitch that
senses closure of the right side
door and that the door is
completely closed.
4. Run an System Reset cycle after
checking/correcting any
instrument problems.
TEMPERATURE OUT OF RANGE The temperature in the counting bath 1. Ensure the sure right side door is
compartment is outside of the closed.
acceptable range. 2. Wait a few minutes.
3. If problem persists, call your
Beckman Coulter Representative.
THE PRINTER IS DISCONNECTED, No or incorrect communication 1. Ensure the cable is properly
SWITCHED OFF, OR HAS NOT BEEN between Printer and instrument connected.
SELECTED 2. Ensure the Printer is turned on.
3. Ensure the Printer is online or
selected.
TIMEOUT OVERFLOW ON RS232 There is a problem with the Check that the protocol that has been
communication or handshaking to the setup in the host transmission screen
host computer.
USER PASSWORD A password is required to perform the Enter user password.
requested action.
WRITE ERROR ON RS232 There is a problem with the Check that the protocol that has been
communication or handshaking to the set up in the host transmission screen
host computer.
XXX NOT REACHING HOME Motor did not reach home sensor.
Note: XXX = name of motor.
7.1-2 PN 4237616B
TROUBLESHOOTING
CHECKING THE MOTORS 7
7.2 CHECKING THE MOTORS
Purpose
Use this procedure to individually verify motor operation is smooth and regular for one or
more of seven different stepper motors including the:
Note: If you need help locating the various assemblies, see Figures A.3-1 and A.3-2. These
figures show the assembly the motor controls, not the actual motor being checked.
r Sample probe motor. This motor is responsible for moving the sample probe up and
down. This is also called the vertical traverse motor. You can verify operation of this
motor without opening any compartments.
r Traverse motor. This motor is responsible for the left/right movement of the sample
probe (inside its housing) over the baths assembly. This motor is also called the
horizontal traverse motor. You need to access the right side compartment to verify
operation of this motor.
r Sample syringe motor. This motor is responsible for aspirating, partitioning, and
delivering the proper volume of sample. You need to access the right side compartment
to verify operation of this motor by observing the sample syringe piston movement.
r Waste syringe motor. This motor is responsible for the draining the baths and various
chambers. It also provides mixing bubbles and the vacuum needed to pull the DIFF
specimen from the DIFF bath towards the flow cell injector syringe. You need to access
the right side compartment to verify operation of this motor by observing waste syringe
piston movement.
r Count syringe motor. This motor is responsible for supplying the aperture vacuum
needed for counting as well as the vacuum needed to fill the diluent reservoir. You need
to access the left side compartment to verify operation of this motor by observing count
syringe piston movement.
r 5diff syringe motor. This motor is responsible for the correct proportioning of the stop
diluent in the DIFF bath and for injecting the sample into the flow cell. You need to
access the left side compartment to verify operation of this motor by observing 5diff
syringe piston movement.
r Reagent syringes motor. This motor is responsible for the correct distribution of the
different reagents including Hgb Lyse, Rinse, Fix, Diluent, and WBC Lyse. You need to
access the left side compartment to verify operation of this motor by observing the piston
movements of the reagent syringes.
B Allen wrenches, 2.5 mm and 3.0 mm , may be required.
PN 4237616B 7.2-1
TROUBLESHOOTING
CHECKING THE MOTORS
Preparation
Determine which motor(s) you want to check so you know which door to open or panel to
remove.
To Check All Motors

2. Remove the left side panel and set it aside. For details, see Heading 4.2.

4. Go to the Motors Check heading that follows.
To Only Check Motors in the Right Side Compartment

To check the sample probe, traverse assembly, sample syringe, or waste syringe motor:
1. Open the right side door of the instrument.

To Only Check Motors in the Left Side Compartment

To check the count syringe, 5diff syringe, or reagent syringes motor:

7.2-2 PN 4237616B
TROUBLESHOOTING
CHECKING THE MOTORS 7
Motors Check
1. From the Main Menu, select DIAGNOSTICS tt HARDWARE SYSTEMS tt MOTORS.
Note: Motors must be checked individually. If more than one motor needs checked, select one
motor and verify its operation before proceeding to the next.
2. From the Motors menu, select the motor you want to check:
1. SAMPLING PROBE (same as Vertical Traverse or Sample Probe)
2. TRAVERSE (same as Horizontal Traverse)
3. SAMPLING SYRINGE (same as Sample Syringe)
4. DRAINING SYRINGE (same as Waste Syringe)
5. COUNTING SYRINGE (same as Count Syringe
6. FLOWCELL SYRINGES (same as 5diff Syringe)
7. DILUTION SYRINGES (same as Reagent Syringes assembly)
3. Verify the motor is operating smoothly and regularly by observing the movement of the
respective components. See Figures A.3-1 and A.3-2 if you need help locating these
assemblies.
r If checking the SAMPLING PROBE motor, make sure the sample probe is moving up
and down properly (vertical traverse movement check).
r If checking the TRAVERSE motor, make sure the left/right movement of the sample
probe (inside its housing) over the baths assembly is correct (horizontal traverse
movement check).
r If checking the SAMPLING SYRINGE motor, make sure the sample syringe piston is
moving up and down properly.
r If checking the DRAINING SYRINGE motor, make sure the waste syringe piston is
r If checking the COUNTING SYRINGE motor, make sure the count syringe piston is
r If checking the FLOWCELL SYRINGE motor, make sure the 5diff syringe piston is
r If checking the DILUTION SYRINGES motor, make sure all five syringe pistons in the
reagent syringes assembly are moving up and down properly.
Wrap Up
When motor performance is acceptable, close all doors and panels:
1. If the right side compartment was opened, close the right side door.
2. If the left side compartment was opened,
a. Close the Main card door. Turn the two captive knobs clockwise to secure the door.
PN 4237616B 7.2-3
TROUBLESHOOTING
CHECKING THE MOTORS
frame.
b. Replace the left side panel and install the four hex screws that secure it to the
instrument frame.
7.2-4 PN 4237616B
TROUBLESHOOTING
CHECKING THE VALVES 7
7.3 CHECKING THE VALVES
Purpose
Use this procedure to verify a valve’s operation is smooth and regular. When activated, all the
valves in the assembly should be activated. There are five valve assemblies in the instrument
including:
r One 11-valve assembly (valve numbers 1 through 11) located in the left side
compartment.
r One 5-valve assembly (valve numbers 12 through 16) located in the left side
compartment.
r One 2-valve assembly (valve numbers 17 and 18) located in the right side compartment.
r One 7-valve assembly (valve numbers 20 through 26) located in the right side
compartment.
r One 5-valve assembly (valve numbers 27 through 31) located in the right side
compartment.
Note: Valve 19 is not used on the AC•T 5diff hematology analyzer.
B Allen wrenches, 2.5 mm and 3.0 mm, may be required.
Preparation
Determine which valve or valves you want to check so you know which door to open or panel
to remove.
To Only Check Valves in the Left Side Compartment

To check one or more valves with a number 1 through 16:

3. Go to the Valves Check heading that follows.
To Only Check Valves in the Right Side Compartment

To check one or more valves with a number 17 or higher:
1. Open the right side door of the instrument.
2. Go to the Valves Check heading that follows.
PN 4237616B 7.3-1
TROUBLESHOOTING
CHECKING THE VALVES
Valves Check
1. From the Main Menu, select DIAGNOSTICS tt HARDWARE SYSTEMS tt VALVES.
2. From the Valves menu, select the range of valves you want to check:
a. 1 to 11 Figure 7.3-1 Valve 1 through 16 Locations

b. 12 to 16
(Valves are located

inside the left
compartment.)
c. 17 and 18 Figure 7.3-2 Valves 17 and 18 Location
(Valves are located

inside the right
compartment, just
above the sample
syringe assembly.)
d. 20 to 26 Figure 7.3-3 Valves 20 through 31 Locations

e. 27 to 31
(Valves are located

inside the right
compartment.)
7.3-2 PN 4237616B
TROUBLESHOOTING
CHECKING THE VALVES 7
Wrap Up
When valve performance is acceptable, close all doors and panels:
1. If the right side compartment was opened, close the right side door.
2. If the left side compartment was opened,
a. Close the Main card door. Turn the two captive knobs clockwise to secure the door.
frame.
b. Replace the left side panel and install the four hex screws that secure it to the
instrument frame.
PN 4237616B 7.3-3
TROUBLESHOOTING
CHECKING THE VALVES
7.3-4 PN 4237616B
CONTENTS
8 PARTS LISTS, 8.1-1
8.1 MASTER PARTS LISTS, 8.1-1
8.2 ILLUSTRATED PARTS, 8.2-1
ILLUSTRATIONS
8.2-6 Right Side Compartment, Lower Rear Area (See Table 8.2-6), 8.2-6
8.2-7 Diluent Reservoir Assembly (See Table 8.2-7), 8.2-7
8.2-8 Count Syringe and Motor Assembly (See Table 8.2-8), 8.2-8
8.2-9 Count Syringe Assembly (See Table 8.2-9), 8.2-9
8.2-10 Count Syringe Motor Assembly (See Table 8.2-10), 8.2-10
8.2-11 Count Syringe Piston Assembly (See Table 8.2-11), 8.2-11
8.2-12 Reagent Syringes and Motor Assembly (See Table 8.2-12), 8.2-12
8.2-13 Reagent Syringes Assembly (See Table 8.2-13), 8.2-13
8.2-14 5diff Syringe and Motor Assembly (See Table 8.2-14), 8.2-14
8.2-15 5diff Syringe Assembly (See Table 8.2-15), 8.2-15
8.2-16 Waste Syringe and Motor Assembly (See Table 8.2-16), 8.2-16
8.2-17 Waste Syringe Assembly (See Table 8.2-17), 8.2-17
8.2-18 Syringe Motor (See Table 8.2-18), 8.2-18
8.2-19 Sample Motor (See Table 8.2-19), 8.2-19
8.2-20 Sample Syringe and Motor Assembly (See Table 8.2-20), 8.2-20
8.2-21 Sample Assembly Syringe (See Table 8.2-21), 8.2-21
8.2-22 Sample Syringe Motor Assembly (See Table 8.2-22), 8.2-22
8.2-23 Syringe Motor Housing Assembly (See Table 8.2-23), 8.2-23
8.2-24 Syringe Motor Guide Block Assembly (See Table 8.2-24), 8.2-24
8.2-25 Sample Probe Retainer and Guide Assembly (See Table 8.2-25), 8.2-25
8.2-26 Sample Probe Retainer (See Table 8.2-26), 8.2-26
8.2-27 Rinse Block Assembly (See Table 8.2-27), 8.2-27
8.2-28 Sample Probe (See Table 8.2-28), 8.2-28
Table 8.2-29), 8.2-29
Table 8.2-30), 8.2-30
Table 8.2-31), 8.2-31
8.2-32 Traverse Vertical Movement Components - Motor (See Table 8.2-32), 8.2-32
Table 8.2-33), 8.2-33
8.2-34 Traverse Horizontal Movement Components - Motor (See Table 8.2-34), 8.2-34
8.2-35 Traverse Horizontal Movement Components - Belt (See Table 8.2-35)t, 8.2-35
(See Table 8.2-36), 8.2-36
8.2-37 Optical Bench Assembly (See Table 8.2-37), 8.2-37
8.2-38 Optical Bench Lamp (See Table 8.2-38), 8.2-38
PN 4237616B 8-i
CONTENTS
8.2-39 DIFF Flow Cell Assembly (SeeTable 8.2-39), 8.2-39

8.2-40 Optics Preamplifier (See Table 8.2-40), 8.2-40
8.2-41 LED Card (See Table 8.2-41), 8.2-41
8.2-42 Bath Enclosure Compartment (See Table 8.2-42), 8.2-42
8.2-43 Bath Enclosure Fan Assembly (See Table 8.2-43), 8.2-43
8.2-44 Bath Enclosure Door Interlock (See Table 8.2-44), 8.2-44
8.2-45 Reagent Heating Coil Assembly (See Table 8.2-45), 8.2-45
8.2-46 Baths Assembly (See Table 8.2-46), 8.2-46
8.2-47 Hgb Photometer Assembly (See Table 8.2-47), 8.2-47
8.2-48 WBC/BASO Bath Assembly (See Table 8.2-48), 8.2-48
8.2-49 Rear Frame Assembly (See Table 8.2-49), 8.2-49
TABLES
8.1-1 Part Categories, 8.1-1
8.1-2 Return Parts, 8.1-1
8.1-3 Nonreturn Parts, 8.1-2
8.1-4 Peripherals, Accessories and Consumables, 8.1-6
8.1-5 Tools, 8.1-7
8.1-6 Fitting Kit Parts PN - XEA311AS, 8.1-8
8.1-7 Screws Kit Parts PN - XEA293AS, 8.1-8
8.1-8 Installation Kit, PN - XEA484AS, 8.1-10
8.1-9 Waste Alarm Kit, PN - 6912680, 8.1-11
8.1-10 6 Month Maintenance Kit, PN - XEA485AS, 8.1-11
8.1-11 1 Year Maintenance Kit, PN - XEA486AS, 8.1-11
8.1-12 Every 2 Years Maintenance Kit, PN - XEA581AS, 8.1-11
8.1-15 Assorted Tools Kit, - PN 6915458, 8.1-12
8.2-6 Right Side Compartment, Lower Rear Area (See Figure 8.2-6), 8.2-6
8.2-7 Diluent Reservoir Assembly (See Figure 8.2-7), 8.2-7
8.2-8 Count Syringe and Motor Assembly (See Figure 8.2-8), 8.2-8
8.2-9 Count Syringe Assembly (See Figure 8.2-9), 8.2-9
8.2-10 Count Syringe Motor Assembly (See Figure 8.2-10), 8.2-10
8.2-11 Count Syringe Piston Assembly (See Figure 8.2-11), 8.2-11
8.2-12 Reagent Syringes and Motor Assembly (See Figure 8.2-12), 8.2-12
8.2-13 Reagent Syringes Assembly (See Figure 8.2-13), 8.2-13
8.2-14 5diff Syringe and Motor Assembly (See Figure 8.2-14), 8.2-14
8.2-15 5diff Syringe Assembly (See Figure 8.2-15), 8.2-15
8.2-16 Waste Syringe and Motor Assembly (See Figure 8.2-16), 8.2-16
8.2-17 Waste Syringe Assembly (See Figure 8.2-17), 8.2-17
8.2-18 Syringe Motor (See Figure 8.2-18), 8.2-18
8.2-19 Sample Motor (See Figure 8.2-19), 8.2-19
8.2-20 Sample Syringe and Motor Assembly (See Figure 8.2-20), 8.2-20
8.2-21 Sample Assembly Syringe (See Figure 8.2-21), 8.2-21
8-ii PN 4237616B
CONTENTS
8.2-22 Sample Syringe Motor Assembly (See Figure 8.2-22), 8.2-22

8.2-23 Syringe Motor Housing Assembly (See Figure 8.2-23), 8.2-23
8.2-24 Syringe Motor Guide Block Assembly (See Figure 8.2-24), 8.2-24
8.2-25 Sample Probe Retainer and Guide Assembly (See Figure 8.2-25), 8.2-25
8.2-26 Sample Probe Retainer (See Figure 8.2-26), 8.2-26
8.2-27 Rinse Block Assembly (See Figure 8.2-27), 8.2-27
8.2-28 Sample Probe (See Figure 8.2-28), 8.2-28
Figure 8.2-29), 8.2-29
Figure 8.2-30), 8.2-30
Figure 8.2-31), 8.2-31
8.2-32 Traverse Vertical Movement Components - Motor (See Figure 8.2-32), 8.2-32
Figure 8.2-33), 8.2-33
8.2-34 Traverse Horizontal Movement Components - Motor (See Figure 8.2-34), 8.2-34
8.2-35 Traverse Horizontal Movement Components - Belt (See Figure 8.2-35), 8.2-35
(See Figure 8.2-36), 8.2-36
8.2-37 Optical Bench Assembly (See Figure 8.2-37), 8.2-37
8.2-38 Optical Bench Lamp (See Figure 8.2-38), 8.2-38
8.2-39 DIFF Flow Cell Assembly (See Figure 8.2-39), 8.2-39
8.2-40 Optics Preamplifier (See Figure 8.2-40), 8.2-40
8.2-41 LED Card (See Figure 8.2-41), 8.2-41
8.2-42 Bath Enclosure Compartment (See Figure 8.2-42), 8.2-42
8.2-43 Bath Enclosure Fan Assembly (See Figure 8.2-43), 8.2-43
8.2-44 Bath Enclosure Door Interlock (See Figure 8.2-44), 8.2-44
8.2-45 Reagent Heating Coil Assembly (See Figure 8.2-45), 8.2-45
8.2-46 Baths Assembly (See Figure 8.2-46), 8.2-46
8.2-47 Hgb Photometer Assembly (See Figure 8.2-47), 8.2-47
8.2-48 WBC/BASO Bath Assembly (See Figure 8.2-48), 8.2-48
8.2-49 Rear Frame Assembly (Figure 8.2-49), 8.2-49
PN 4237616B 8-iii
CONTENTS
8-iv PN 4237616B
8PARTS LISTS 8
8.1 MASTER PARTS LISTS
The parts listed in this section are divided into tables by category. Table 8.1-1 lists the
categories and their table numbers.
Within each table the part numbers are listed in numeric order. When applicable, a
component is cross referenced to its illustration in Heading 8.2.
Table 8.1-1 Part Categories
Category Table Category Table

Return Parts 8.1-2 Waste Alarm Kit, PN - 6912680 8.1-9
Nonreturn Parts 8.1-3 6 Month Maintenance Kit, PN - XEA485AS 8.1-10
Peripherals, Accessories and Consumables 8.1-4 1 Year Maintenance Kit, PN - XEA486AS 8.1-11
Tools 8.1-5 Every 2 Years Maintenance Kit, PN - XEA581AS 8.1-12
Fitting Kit Parts PN - XEA311AS 8.1-6 100 mN-m Torque Driver Kit, - PN 6915456 8.1-13
Screws Kit Parts PN - XEA293AS 8.1-7 400 mN-m Torque Driver Kit, - PN 6915457 8.1-14
Installation Kit, PN - XEA484AS 8.1-8 Assorted Tools Kit, - PN 6915458 8.1-15
Table 8.1-2 Return Parts
Part Number Description Figure Item

DBN004A Power Supply, module
XAA423BS Preamp, optical signal assembly 8.2-40 1
XAA477CS PCB, Main card (without software)
XAA427BS PCB, Motor interface card
XAA461A PCB, Keypad/LCD Display
XDA600A Optical bench, module
PN 4237616B 8.1-1
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-3 Nonreturn Parts

1017413 Velcro (loop) strip, precut (2 in. x 1 in.), 2 each (used with installation of waste alarm)
1017414 Velcro (hook) strip, precut (2 in. x 1 in.), 2 each (used with installation of waste alarm)
3202035 Tubing, 035 clear polyurethane, 2-inch piece (used with installation of waste alarm)
6011001 Tie wrap, small
6011002 Tie wrap, large
6216308 Feed-through fitting (used with installation of waste alarm)
6912680 Waste alarm, kit. Table 8.1-9 lists the contents of this kit.
CAE006A Switch, microswitch XC5-81-82 8.2-42 3
8.2-44 3
CAE010A Switch, microswitch XC5-81 8.2-8 7
8.2-16 7
8.2-22 5
CAY012A LCD, display screen
DAC011A Cable, power, Europe
DAC012A Cable, power USA
DAD113A Cable, motor interface to Main Card
DAJ007A Lamp assembly for Optical module 8.2-38 1
DBK004A Holder, adhesive, tie wrap, o.d.3 mm, package of 10
DZZ015A Ferrite shield (for printer cable)
DZZ018A Ferrite shield (for coax cables)
EAC008A Fitting, antirotation washer, package of 5 8.2-49 11
EAC010A Fitting, Luer, female, i.d. 3 mm, package of 5 8.2-49 10
EAC019A Fitting, Luer, male, i.d. 3 mm, package of 5
EAE005AS Tubing, Tygon, 1.016 mm i.d. (0.040 in.), 2 meter length
EAE008AS Tubing, Tygon, 2.05 mm i.d. (0.081 in.), 2 meter length 8.2-7 3
EAE011AS Tubing, crystal, 3x6 mm (i.d. x o.d.), 10 meter length
EAE028AS Tubing, crystal, 4x6 mm (i.d. x o.d.), 2 meter length
FAA040A O-ring, 5diff syringe, 12.1 mm diameter, package of 10 8.2-15 2
FAA046A O-ring, coaxial cable, package of 10
FAA053A O-ring, probe rinse block, package of 10 8.2-27 3
FAA064A O-ring, sample syringe, package of 10 8.2-21 2
FAA065A O-ring, reagent syringe, 6.3 mm diameter, package of 10 8.2-13 8
8.1-2 PN 4237616B
PARTS LISTS
MASTER PARTS LISTS 8
Table 8.1-3 Nonreturn Parts (Continued)

FAA066A O-ring, bath drain/debubble chamber, package of 12 8.2-46 10
8.2-48 10
FAA067A O-ring, 5diff syringe, 2.4 mm diameter, package of 10 8.2-15 3
FAK001A Aperture, RBC/Plt, 50 µ 8.2-46 4
FAK003A Aperture, WBC/BASO, 80 µ 8.2-48 4
FAL009A Nut, shock mount, for most subassemblies, package of 12 8.2-8 1
8.2-16 1
8.2-22 10
FAL010A Nut, shock mount, for optics module, package of 12
FAM004A Foot, plastic, main chassis, package of 8
FBL001A Stopper, rubber with two holes, used for diluent pickup
FBR011A Belt, probe vertical, 364 tooth 8.2-30 1
FBR012A Belt, traverse horizontal, 544 tooth 8.2-35 1
GAK302A Cap, reagent bottle, 40 mm diameter, package of 12
GAL094A Chamber, diluent reservoir 8.2-7 4
GAL098A Sleeve, tubing/fitting compression collar
GBC015A Clip, long isolator chamber holder, package of 10 8.2-45 6
GBC030A Piston, reagent syringe, large 8.2-13 3
GBC031A Piston, reagent syringe, lyse 8.2-13 7
GBG003A Chamber, drain and debubble 8.2-46 12
8.2-48 12
GBG007A Diffuser, drain and debubble, set of 4 8.2-46 11
8.2-48 11
GBG013A Drip tray, overflow, bath enclosure
GBG022A Cover, bath enclosure
GBG205A Cover, solenoid drivers, bank 27-31 8.2-42 7
GBG033A Syringe, reagent, body 8.2-13 1
GBG037A Syringe, 5diff, body 8.2-15 1
GBG040A Piston, 5diff syringe 8.2-15 5
GBG042A Syringe, 5diff, O-ring collar 8.2-15 4
GBG044A Syringe, sample, body 8.2-21 1
GBG048A Syringe, sample, O-ring collar 8.2-21 3
GBG052A Piston, count/waste syringe 8.2-11 4
GBG053A Syringe, count, body 8.2-9 3
GBG054A Syringe, waste, body 8.2-17 4
GBG090A Sample probe, rinse block 8.2-27 2
PN 4237616B 8.1-3
PARTS LISTS
MASTER PARTS LISTS

GBG091A Sample probe, guide 8.2-27 4
GBG093A Pulley, belt freewheel 8.2-31 4
8.2-36 3
GBG138A Key, right side door
GBG144A Pickup, reagent bottle, 27 mm stopper
GBG145A Pickup, reagent bottle, 20 mm stopper
GBG147A Drip tray, reagents syringe
GBG155A Cap, reagent bottle, 25 mm diameter, package of 4
GBG156A O-ring, aperture, package of 12 8.2-46 5
8.2-48 5
GBG157A Counting head (internal electrode) 8.2-46 7
8.2-48 7
GBG210A Syringe body (new style)
GBG211A Chamber, counting syringe (new style)
GBG212A Chamber, drain syringe (new style)
GBG219A Anti-extrusion ring 8.2-9 5
8.2-17 5
GBG225A Keypad, silicone
HAE026B Label, valves, 1-10, package of 10
HAX0012 Label, tubing, reagent lines
HAX0013 Label, tubing, bath enclosure lines
XAA468A PCB, LED card, aspiration indicator 8.2-41 2
XBA144A Cable, reagent ground fitting
XBA193A Cable, coaxial, flow cell optical
XBA386A Cable, bath enclosure harness
XBA389A Photometer, HGB diode and preamp assembly 8.2-47 4
XBA390A Motor, for syringe assembly 8.2-10 1
8.2-18 1
8.2-22 1
XBA391A Motor, traverse, horizontal 8.2-34 1
XBA392A Motor, sample probe, vertical 8.2-31 9
8.2-32 1
XBA393A Fan, main chassis, 24 V 8.2-49 6
8.1-4 PN 4237616B
PARTS LISTS

XBA394A Sensor, traverse horizontal, home 8.2-36 7
XBA395A Sensor, fluid, bath drain
XBA396A Sensor, sample probe vertical, home 8.2-33 1
XBA398B Cable, coaxial, with RBC/WBC bath electrode 8.2-46 2
8.2-48 2
XBA399A Cable, coaxial, flow cell volume
XBA403A Shield, flow cell tubing ground 8.2-39 2
XCA166A Chamber, isolator (long) 8.2-45 5
XCA167A Chamber, isolator (small)
XDA481B Valve, liquid. 2-way, normally closed, w/o coil
XDA483B Valve, liquid, 3-way, w/o coil
XDA591AS Syringe, 5diff assembly 8.2-14 2
8.2-15 10
XDA592AS Syringe, reagent assembly 8.2-12 3
8.2-13 9
XDA593AS Syringe, sampling assembly 8.2-20 2
XDA594AS Motor, assembly, for 5diff syringe 8.2-14 1
XDA595AS Motor, assembly, for reagent syringe 8.2-12 1
XDA596AS Motor, assembly, for sample syringe 8.2-20 1
XDA597BS Syringe, waste - complete assembly 8.2-16 2
8.2-17 1
XDA598BS Syringe, vacuum - complete assembly 8.2-8 2
8.2-9 1
XDA601AS Flow cell, assembly 8.2-39 1
XDA602B Bath, assembly, 3-baths and rinse chamber (includes long coax cable) 8.2-47 3
XDA605A Reservoir, diluent, assembly 8.2-6 1
8.2-7 8
XDA610B Bath, WBC/BASO (includes long coax cable) 8.2-47 1
XDA611CS Valve, liquid, 11-valve assembly (1-11) 8.2-1 1
8.2-19 2
8.2-45 4
XDA616AS Piston, 5diff syringe, 190 µL needle 8.2-15 9
PN 4237616B 8.1-5
PARTS LISTS
MASTER PARTS LISTS

XDA617AS Piston, sample syringe, 100 µL needle 8.2-21 6
XDA618AS Guide, sample probe retainer 8.2-26 1
XDA619AS Probe, sample 8.2-28 1
XDA621A O-ring, with matched washer, count/waste syringe, package of 10 8.2-9 2
8.2-13 2
8.2-17 2
XDA622A O-ring, with matched washer, for reagent syringe, package of 10
XDA623AS Panel, reagent/waste fittings assembly 8.2-49 14
XDA625AS Heater, reagent coil assembly 8.2-45 1
XDA626AS Cover, diluent reservoir 8.2-7 2
XDA657B Bath assembly, including WBC/Baso bath (includes long coax cable)
XEA018A Pickup tube, diluent (needs stopper)
XEA286AS Kit, O-ring and washer 8.2-7 7
XEA293AS Kit, screws. Table 8.1-7 lists the contents of this kit.
Note: The Screws kit contains the most common screws used in the AC•T 5diff
analyzer, plus nuts and washers.
XEA311AS Kit, fittings. Table 8.1-6 lists the contents of this kit.
Note: The Fitting kit contains the most common pneumatic and hydraulic parts used in
the AC•T 5diff analyzer.
XEA484A Kit, installation (comes with instrument).Table 8.1-8 lists the contents of this kit.
XEA485AS Kit, maintenance, 6 month. Table 8.1-10 lists the contents of this kit.
XEA486AS Kit, maintenance, 1 year. Table 8.1-11 lists the contents of this kit.
XEA487AS Fan, bath enclosure temperature control 8.2-43 2
XEA488AS Cable, heating coil harness
XEA581AS Kit, maintenance, every two years. Table 8.1-12 lists the contents of this kit.
XEA616AS Tubing, with sleeve, for flow cell sample port 6
XEA643AS Kit, software PROMS, AC•T 5diff v1.03
Table 8.1-4 Peripherals, Accessories and Consumables
Part Number Description

2016891 Ribbon, black print, for EPSON LX300
4237615 Operator’s Manual, AC•T 5diff (English)
4237616 Service Manual, AC•T 5diff (English only)
4237630 Operator’s Manual, AC•T 5diff (French)
4237631 Operator’s Manual, AC•T 5diff (Italian)
4237632 Operator’s Manual, AC•T 5diff (German)
8.1-6 PN 4237616B
PARTS LISTS
Table 8.1-4 Peripherals, Accessories and Consumables (Continued)

4237633 Operator’s Manual, AC•T 5diff (Spanish)
4237634 Operator’s Manual, AC•T 5diff (Chinese)
7547175 Calibrator, AC•T 5diff Cal
7547176 Control, AC•T 5diff
8547168 Reagent, AC•T 5diff Hgb Lyse
8547169 Reagent, AC•T 5diff Diluent
8547170 Reagent, AC•T 5diff WBC Lyse
8547171 Reagent, AC•T 5diff Fix
8547176 Reagent, AC•T 5diff Rinse
CBE070AS Printer Head, Epson LX300+
FBH016A Cover, dust
LAD002AS Latex particles, RBC/PLT (used for all procedures)
LAM004A Grease, silicone
XAA473A Printer, Epson LX300+ 220 V
XAA476A Printer, Epson LX300+ 110 V
XEA019A Grease, silicone, for syringe assembly
XEA381AS Grease, for mechanical assemblies
XEA587AS Bar code reader option
Table 8.1-5 Tools

5415407 Set, Allen wrenches, balldriver, metric
5450517 Screwdriver, 2.5 mm hex, balldriver
5450518 Screwdriver, 3.0 mm hex, balldriver
5450519 Bit, 2.5 mm hex balldriver, for 1/4 inch drive
5450520 Bit, 3.0 mm hex balldriver, for 1/4 inch drive
5450521 Screwdriver, torque, preset to 100 mN-m (14.2 oz.f-in), 1/4 inch drive
5450522 Screwdriver, torque, preset to 400 mN-m (56.8 oz.f-in), 1/4 inch drive
5450532 Screwdriver, T10 Torx
5450533 Bit, T10 Torx, for 1/4 inch drive
5450535 Screwdriver, small, Phillips 00
5450536 Magnifier, jeweller’s loupe, 5X with 2 in. focal length
5450537 Extractor, chip remover, for U42 and U43
5450540 Gauge, feeler adjustment, 9.4 mm and 3.0 mm
PN 4237616B 8.1-7
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-5 Tools (Continued)

6915456 Kit, 100 mN-m torque driver with 3 bits. Table 8.1-13 lists the contents of this kit.
6915457 Kit, 400 mN-m torque driver with 3 bits. Table 8.1-14 lists the contents of this kit.
6915458 Kit, assorted tools for AC•T 5diff. Table 8.1-15 lists the contents of this kit.
MAB002A Allen wrench, L-shape, 2.5 mm
MAB018A Allen wrench, L-shape, 3.0 mm
MAB090A Torx key, L-shape, T10
XDA555AS Knob, used to adjust flowcell
XEA585AS Torque screwdriver, A300, adjustable torque, 100 mN-m, 1/8 inch drive
XEA587AS Torque screwdriver, A301, adjustable torque, 400 mN-m, 1/4 inch drive
Table 8.1-6 Fitting Kit Parts PN - XEA311AS
Part Number Description Quantity

EAA005A Fitting, straight connector, 01.6 mm 1/8 10
EAA006A Fitting, straight connector 10
EAA009A Fitting, bent connector, i.d.1.5 10/32 10
EAA013A Fitting, bent connector, 1/8 10
EAA014A Fitting, straight connector, 1/8 10
EAB002A Fitting, L-shaped connector 10
EAB003A Fitting, connector04 mm H19.5 20
EAB005A Fitting, T-connector, 01.6 mm T10-6 30
EAB006A Fitting, T-connector, 02.3 mm 30
EAB009A Fitting, straight connector, 01.6 mm 30
EAB010A Fitting, straight connector, 02.3 mm 30
EAB014A Fitting, straight connector, Y, 01.5 mm/1.5 mm 10
EAB021A Fitting, Y-connector, 03 mm 3
EAB026A Fitting, Y-connector, 02.5 mm 3
EAB035A Fitting, T-connector, 02.3 mm T220-6 10
Table 8.1-7 Screws Kit Parts PN - XEA293AS

KAA002A Screw, hex M3x6 20
8.1-8 PN 4237616B
PARTS LISTS
Table 8.1-7 Screws Kit Parts PN - XEA293AS (Continued)

KAB002A Screw, FHC M3x6 20
KAB016A Screw, torx M3x8 5
KAC002A Screw, TC M3x5 20
KAE003A Screw, BHC M4x12 screw 20
KAH001A Nut, HU M3 20
KAH024A Nut, 1/4-28 (US std) 10
KAJ001A Washer, M diameter 0.3 20
KAJ007A Washer, AZ diameter 0.3 20
KAJ008A Washer, Z diameter 0.4 20
KAJ009A Washer, AZ diameter 0.5 20
KAJ010A Washer, W grower diameter 0.5 20
KAM002A Collar, plastic, M3x6 Lg.8 10
KAM004A Collar, plastic, M3.5x6 Lg.6 10
KAM011A Collar, plastic, M3.5x6 Lg.5.7 10
KAM017A Collar, brass, M3.5x6 Lg.3 10
PN 4237616B 8.1-9
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-8 Installation Kit, PN - XEA484AS

DAJ007A Lamp, 20 W, 9.5 Vdc 1
DBH001A Tie wrap LA=2.4 L=92 3
DBH002A Tie wrap LA=3.6 L=140 3
DBK003A Adhesive holder 3 mm 1
EAE007A Tygon tube 1.52 mm (0.060 inch) 2
EAE011A Cristal tube 3 mm (i.d.) x 6 mm (o.d.) 4
EAE028A Cristal tube 4 mm (i.d.) x 6 mm (o.d.) 4
EAC019A Luer male connector, i.d. 3 mm 3
EAC010A Luer bushing, i.d. 3 mm 2
EAB021A Fitting, Y-connector 3 mm 1
EAB033A Fitting, T-connector (small) 1
EAB035A Fitting, T-connector 2.3 mm T220-6 inches 1
FAA053A O-ring, 1.40 x 1.25, fluocarbon 1
FBH016A Instrument cover 1
FBL001A Rubber cap 2 holes 1
GBG138A Tool, right access panel fasteners 1
GBG156A EPO counting head Joint 2
GBG144A Reagent straw cap 027 3
GBG145A Reagent straw cap 020 1
GAK302A Factory bottle cap 3
GBG155A Cap 025 1
MAB018A Bent allen key 3 mm 1
MAB002A Bent allen key 2.5 mm 1
MAB090A Bent Torx key T10 1
XDA619AS Equipped probe 1
XDA483B Valve 3V (no solenoid) 1
XDA621A O-ring 30.80 x 3.80 + wedge 1
XEA018A Diluent pickup tube, 360 mm length 1
XEA019A Grease KM 1011 1
8.1-10 PN 4237616B
PARTS LISTS
Table 8.1-9 Waste Alarm Kit, PN - 6912680

6706397 Alarm, waste, with 9V battery 1
6856742 Sensor, float 1
Table 8.1-10 6 Month Maintenance Kit, PN - XEA485AS

FAA053A O-ring, fluorocarbon, rinse block assembly 1
FAA065A O-ring, silicone, Hgb Lyse reagent syringe 1
XDA621A O-ring and washer, matched set, waste or count syringe 2
XDA622A O-ring and washer, matched set, for all reagent syringes (except Hgb Lyse syringe) 4
XEA019A Silicone grease 1
Table 8.1-11 1 Year Maintenance Kit, PN - XEA486AS

DAJ007A Lamp, optical bench, 20 W, 9.5 Vdc 1
FAA040A O-ring, silicone, 5diff syringe 1
FAA046A O-ring, coaxial cable, counting head 2
FAA064A O-ring, fluorocarbon, sample syringe 2
FAA066A O-ring, silicon, draining bath 4
FAA067A O-ring, fluorocarbon, 5diff syringe 4
GBG156A O-ring, aperture, counting head 4
XBA399A Cable, coaxial, DIFF flow cell 1
XEA286AS O-ring and washer, matched set, diluent reservoir 1
Table 8.1-12 Every 2 Years Maintenance Kit, PN - XEA581AS

GBC031A Piston, Hgb Lyse reagent syringe 1
GBC030A Pistons, reagent syringes assembly 4
GBG052A Piston, waste or count syringe 2
GBG091A Block, sample probe guide 1
XDA619AS Probe, sample 1
PN 4237616B 8.1-11
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-13 100 mN-m Torque Driver Kit, - PN 6915456

5450519 Bit, 2.5 mm hex balldriver, for 1/4 inch drive 1
5450521 Screwdriver, torque, preset to 100 mN-m (14.2 oz.f-in), 1/4 inch drive 1
5450533 Bit, T10 Torx, for 1/4 inch drive 1
Table 8.1-14 400 mN-m Torque Driver Kit, - PN 6915457

5450522 Screwdriver, torque, preset to 400 mN-m (56.8 oz.f-in), 1/4 inch drive 1
5450533 Bit, T10 Torx, for 1/4 inch drive 1
Table 8.1-15 Assorted Tools Kit, - PN 6915458

5415407 Set, Allen wrenches, balldriver, metric 1
5450517 Screwdriver, 2.5 mm hex, balldriver 1
5450518 Screwdriver, 3.0 mm hex, balldriver 1
5450532 Screwdriver, T10 Torx 1
5450535 Screwdriver, small, Phillips 00 1
5450536 Magnifier, jeweller’s loupe, 5X with 2 in. focal length 1
5450537 Extractor, chip remover, for U42 and U43 1
8.1-12 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
8.2 ILLUSTRATED PARTS
The exploded views in this section are shown for informational purposes only. Many of the
subcomponents are not available. Consult the MASTER PARTS LISTS for part availability.
Figure 8.2-1 11-Valves Assembly (See Table 8.2-1)
LML006AC
Table 8.2-1 11-Valves Assembly (See Figure 8.2-1)
Item Part Number Description

1 XDA611CS Valve, liquid, 11-valve assembly (1-11)
PN 4237616B 8.2-1
PARTS LISTS
ILLUSTRATED PARTS
LML004AC

8.2-2 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
LML002AC

PN 4237616B 8.2-3
PARTS LISTS
ILLUSTRATED PARTS
LML005AC

8.2-4 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
LML003AC

PN 4237616B 8.2-5
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-6 Right Side Compartment, Lower Rear Area (See Table 8.2-6)
LML905AC
Table 8.2-6 Right Side Compartment, Lower Rear Area (See Figure 8.2-6)

1 XDA605A Reservoir, diluent, assembly
2 KAA013A Screw, hex M4x6
8.2-6 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-7 Diluent Reservoir Assembly (See Table 8.2-7)
LML223AC
Table 8.2-7 Diluent Reservoir Assembly (See Figure 8.2-7)

2 XDA626AS Cover, diluent reservoir
3 EAE008A Tubing, Tygon, 2.05 mm i.d. (0.081 in.), 2 meter length
4 GAL094A Chamber, diluent reservoir
5 GBG056A Brace, reservoir, diluent
7 XEA286AS Kit, O-ring and washer
8 XDA605A Reservoir, diluent, assembly
PN 4237616B 8.2-7
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-8 Count Syringe and Motor Assembly (See Table 8.2-8)
LML214AC
Table 8.2-8 Count Syringe and Motor Assembly (See Figure 8.2-8)

1 FAL009A Nut, shock mount, package of 12 - for most subassemblies
2 XDA598AS Syringe, vacuum - complete assembly
XDA598BS Syringe, vacuum - complete assembly (includes anti-extrusion ring)
4 KZZ022A Screw, auto-threaded
5 GBG027A Plate, microswitch, motor
7 CAE010A Switch, microswitch XC5-81
8.2-8 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-9 Count Syringe Assembly (See Table 8.2-9)
5 2
4 (4x)
7616124B
Table 8.2-9 Count Syringe Assembly (See Figure 8.2-9)

1 XDA598AS Syringe, vacuum - complete assembly
XDA598BS Syringe, vacuum - complete assembly (includes anti-extrusion ring)
2 XDA621A O-ring, with matched washer, count/waste syringe, package of 10
3 GBG053A Syringe, count, body
4 KA017A Screw, hex M4x16
5 GBG219A Anti-extrusion ring
PN 4237616B 8.2-9
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-10 Count Syringe Motor Assembly (See Table 8.2-10)
LML212AC
Table 8.2-10 Count Syringe Motor Assembly (See Figure 8.2-10)

1 XBA390A Motor, for syringe assembly
2 GBG050A Body, count syringe
3 Piston assembly, count syringe (See Figure 8.2-11)
5 Grounding wire
6 GBG029A Syringe screw
7 KAD016A Screw, HC M4x6
8.2-10 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-11 Count Syringe Piston Assembly (See Table 8.2-11)
LML210AC
Table 8.2-11 Count Syringe Piston Assembly (See Figure 8.2-11)

2 GBG029A Syringe nut
3 GBG051A Guide plate, count/waste syringe
4 GBG052A Piston, count/waste syringe
PN 4237616B 8.2-11
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-12 Reagent Syringes and Motor Assembly (See Table 8.2-12)
LML216AC
Table 8.2-12 Reagent Syringes and Motor Assembly (See Figure 8.2-12)

1 XDA595AS Motor, assembly - for reagent syringe
2 Motor - for reagent syringe
3 XDA592AS Syringes, reagent assembly
5 GBG035A Guide plate, reagent syringe
8.2-12 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-13 Reagent Syringes Assembly (See Table 8.2-13)
x4
9
x4
x9
LML202AC
Table 8.2-13 Reagent Syringes Assembly (See Figure 8.2-13)

1 GBG033A Syringe, reagent, body
3 GBC030A Piston, reagent syringe, large
4 KAB017A Screw, torx M3x12
6 GBG034A Syringe, reagent, top
7 GBC031A Piston, reagent syringe, lyse
8 FAA065A O-ring, reagent syringe, 6.3 mm diameter, package of 10
9 XDA592AS Syringe, reagent assembly
PN 4237616B 8.2-13
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-14 5diff Syringe and Motor Assembly (See Table 8.2-14)
LML215AC
Table 8.2-14 5diff Syringe and Motor Assembly (See Figure 8.2-14)

1 XDA594AS Motor, assembly - for 5diff syringe
2 XDA591AS Syringe, 5diff assembly
4 GBG039A Guide plate, syringe
8.2-14 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-15 5diff Syringe Assembly (See Table 8.2-15)
LML201AC
Table 8.2-15 5diff Syringe Assembly (See Figure 8.2-15)

1 GBG037A Syringe, 5diff, body
2 FAA040A O-ring, 5diff syringe, 12.1 mm diameter, package of 10
3 FAA067A O-ring, 5diff syringe, 2.4 mm diameter, package of 10
4 GBG042A Syringe, 5diff, O-ring collar
5 GBG040A Piston, 5diff syringe
6 GBG038A Syringe top
9 XDA616AS Piston, 5diff syringe, 190 µL needle
10 XDA591AS Syringe, 5diff assembly
PN 4237616B 8.2-15
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-16 Waste Syringe and Motor Assembly (See Table 8.2-16)
LML222AC
Table 8.2-16 Waste Syringe and Motor Assembly (See Figure 8.2-16)

2 XDA597AS Syringe, waste - complete assembly
XDA597BS Syringe, waste - complete assembly (includes anti-extrusion ring)
8.2-16 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-17 Waste Syringe Assembly (See Table 8.2-17)
5 2
3 (4x)
4 7616125B
Table 8.2-17 Waste Syringe Assembly (See Figure 8.2-17)

1 XDA597AS Syringe, waste - complete assembly
XDA597BS Syringe, waste - complete assembly (includes anti-extrusion ring)
4 GBG054A Syringe, waste, body
5 BGG219A Anti-extrusion ring
PN 4237616B 8.2-17
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-18 Syringe Motor (See Table 8.2-18)
LML207AC
Table 8.2-18 Syringe Motor (See Figure 8.2-18)

1 XBA390A Motor - for syringe assembly
8.2-18 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-19 Sample Motor (See Table 8.2-19)
LML415AC
Table 8.2-19 Sample Motor (See Figure 8.2-19)

1 GBG086A Support plate, sample syringe
4 Sample Syringe and Motor Assembly (See Figure 8.2-20)
PN 4237616B 8.2-19
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-20 Sample Syringe and Motor Assembly (See Table 8.2-20)
LML217AC
Table 8.2-20 Sample Syringe and Motor Assembly (See Figure 8.2-20)

1 XDA596AS Motor, assembly - for sample syringe (See Figure 8.2-22)
2 XDA593AS Syringe, sampling assembly (See Figure 8.2-21)
8.2-20 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-21 Sample Assembly Syringe (See Table 8.2-21)
x2
x3
LML204AC
Table 8.2-21 Sample Assembly Syringe (See Figure 8.2-21)

1 GBG044A Syringe, sample, body
2 FAA064A O-ring, sample syringe, package of 10
3 GBG048A Syringe, sample, O-ring collar
4 GBG045A Syringe top, sampling
6 XDA617AS Piston, sample syringe, 100 µL needle
7 XDA593AS Syringe, sampling assembly
PN 4237616B 8.2-21
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-22 Sample Syringe Motor Assembly (See Table 8.2-22)
LML209AC
Table 8.2-22 Sample Syringe Motor Assembly (See Figure 8.2-22)

1 XBA390A Motor - for syringe assembly
3 Syringe Motor Housing Assembly (See Figure 8.2-23)
8 Grounding wire
11 GBG025A Syringe screw
8.2-22 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-23 Syringe Motor Housing Assembly (See Table 8.2-23)
LML208AC
Table 8.2-23 Syringe Motor Housing Assembly (See Figure 8.2-23)

1 GBG028A Motor housing, syringe
2 KAD016A Screw, HC M4x6
3 Syringe Motor Guide Block (See Figure 8.2-24)
4 GBG030A Motor guide, assembly
PN 4237616B 8.2-23
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-24 Syringe Motor Guide Block Assembly (See Table 8.2-24)
LML206AC
Table 8.2-24 Syringe Motor Guide Block Assembly (See Figure 8.2-24)

2 GBG029A Syringe nut
3 GBG031A Motor guide block, syringe
8.2-24 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-25 Sample Probe Retainer and Guide Assembly (See Table 8.2-25)
LML409AC
Table 8.2-25 Sample Probe Retainer and Guide Assembly (See Figure 8.2-25)

1 GBG162A Centering sheet, steel
3 GBG081A Needle axis
PN 4237616B 8.2-25
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-26 Sample Probe Retainer (See Table 8.2-26)
LML403AC
Table 8.2-26 Sample Probe Retainer (See Figure 8.2-26)

1 XDA618AS Guide, sample probe retainer
8.2-26 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-27 Rinse Block Assembly (See Table 8.2-27)
LML411AC
Table 8.2-27 Rinse Block Assembly (See Figure 8.2-27)

1 Traverse vertical movement components - Home sensor (See Figure 8.2-33)
2 GBG090A Sample probe, rinse block
3 FAA053A O-ring, probe rinse block, package of 10
4 GBG091A Sample probe, guide
PN 4237616B 8.2-27
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-28 Sample Probe (See Table 8.2-28)
LML412AC
Table 8.2-28 Sample Probe (See Figure 8.2-28)

1 XDA619AS Probe, sample
8.2-28 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-29 Traverse Vertical Movement Components - Belt Retainer (See Table 8.2-29)
LML410AC
Table 8.2-29 Traverse Vertical Movement Components - Belt Retainer (See Figure 8.2-29)

2 Belt, probe vertical, assembly, 364 tooth
PN 4237616B 8.2-29
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-30 Vertical Traverse Vertical Movement Components - Belt (See Table 8.2-30)
LML404AC
Table 8.2-30 Vertical Traverse Vertical Movement Components - Belt (See Figure 8.2-30)

1 FBR011A Belt, probe vertical, 364 tooth
8.2-30 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-31 Traverse Vertical Movement Components - Motor and Pulley (See Table 8.2-31)
x2
8
x2
7
9
x3
2
3
5 4 LML407AC
6
Table 8.2-31 Traverse Vertical Movement Components - Motor and Pulley (See Figure 8.2-31)

2 DBE014A Wire guide
3 GBC147A Pulley holder
4 GBG093A Pulley, belt freewheel
5 FAG011A Bearing
8 KAJ001A Washer, M diameter 0.3
9 XBA392A Motor, sample probe, vertical
PN 4237616B 8.2-31
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-32 Traverse Vertical Movement Components - Motor (See Table 8.2-32)
LML400AC
Table 8.2-32 Traverse Vertical Movement Components - Motor (See Figure 8.2-32)

1 XBA392A Motor, sample probe, vertical
8.2-32 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-33 Traverse Vertical Movement Components - Home Sensor (See Table 8.2-33)
LML406AC
Table 8.2-33 Traverse Vertical Movement Components - Home Sensor (See Figure 8.2-33)

1 XBA396A Sensor, home, sample probe vertical
2 KAA002AA Screw, hex M3x6
PN 4237616B 8.2-33
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-34 Traverse Horizontal Movement Components - Motor (See Table 8.2-34)
LML413AC
Table 8.2-34 Traverse Horizontal Movement Components - Motor (See Figure 8.2-34)

1 XBA391A Motor, traverse, horizontal
2 GBG095A Nut, plate M5
8.2-34 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-35 Traverse Horizontal Movement Components - Belt (See Table 8.2-35)t
LML402AC
Table 8.2-35 Traverse Horizontal Movement Components - Belt (See Figure 8.2-35)

1 FBR012A Belt, traverse horizontal, 544 tooth
PN 4237616B 8.2-35
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-36 Traverse Horizontal Movement Components - Free Wheel and Home Sensor
(See Table 8.2-36)
LML414AC
Table 8.2-36 Traverse Horizontal Movement Components - Free Wheel and Home Sensor
(See Figure 8.2-36)

1 Frame assembly
2 GBG146A Freewheel strengthener
3 GBG093A Pulley, belt freewheel
4 FAG011A Bearing
7 XBA394A Sensor, home, traverse horizontal
8.2-36 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-37 Optical Bench Assembly (See Table 8.2-37)
LML060AB
Table 8.2-37 Optical Bench Assembly (See Figure 8.2-37)

1 XDA600AS Optical bench assembly
PN 4237616B 8.2-37
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-38 Optical Bench Lamp (See Table 8.2-38)
LML061AC
Table 8.2-38 Optical Bench Lamp (See Figure 8.2-38)

1 DAJ007A Optical bench lamp
8.2-38 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-39 DIFF Flow Cell Assembly (See Table 8.2-39)
LML000AC
Table 8.2-39 DIFF Flow Cell Assembly (See Figure 8.2-39)

1 XDA601AS Flow cell, assembly
2 XBA403A Shield, flow cell tubing ground
PN 4237616B 8.2-39
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-40 Optics Preamplifier (See Table 8.2-40)
LML064BC
Table 8.2-40 Optics Preamplifier (See Figure 8.2-40)

1 XAA423BS Preamp, optical signal assembly
8.2-40 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-41 LED Card (See Table 8.2-41)
LML219AC
Table 8.2-41 LED Card (See Figure 8.2-41)

2 XAA468A PCB, LED card, aspiration indicator
PN 4237616B 8.2-41
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-42 Bath Enclosure Compartment (See Table 8.2-42)
6(X6)
4 (X2)
7616126B 2 3 1
Table 8.2-42 Bath Enclosure Compartment (See Figure 8.2-42)

1 GBG020A Nut, plate, bath enclosure compartment
2 GBD498A Nut, plate, microswitch
3 CAE006A Switch, microswitch XC5-81-82
7 GBG205A Cover, solenoid drivers, bank 27-31
8.2-42 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-43 Bath Enclosure Fan Assembly (See Table 8.2-43)
LML901AC
Table 8.2-43 Bath Enclosure Fan Assembly (See Figure 8.2-43)

1x KAA015A Screw, hex M4x8
2 XEA487AS Fan, bath enclosure temperature control
3 KAN023A Collar
4 GBG019A Insulated plate
5 GBG063A Steel
PN 4237616B 8.2-43
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-44 Bath Enclosure Door Interlock (See Table 8.2-44)
LML306AC
Table 8.2-44 Bath Enclosure Door Interlock (See Figure 8.2-44)

1 GBG012A Backplate, bath enclosure
3 CAE006A Switch, microswitch XC5-81-82
4 GBD498A Nut, microswitch
5 GBG018A Plate, bath enclosure, rear isolation
6 DBE026A Thrust
7 FAM006A Bushing
8.2-44 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-45 Reagent Heating Coil Assembly (See Table 8.2-45)
LML304AC
Table 8.2-45 Reagent Heating Coil Assembly (See Figure 8.2-45)

1 XDA625AS Heater, reagent coil assembly
2 GBG009A Holder clip, chamber
3 GBG014A Chamber plate, insulated
5 XCA166A Chamber, isolator (long)
6 GBC015A Clip, long isolator chamber holder, package of 10
PN 4237616B 8.2-45
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-46 Baths Assembly (See Table 8.2-46)
LML224AC
Table 8.2-46 Baths Assembly (See Figure 8.2-46)

2 XBA398B Cable, coaxial, with RBC/WBC bath electrode
3 GBG001A Four-bath unit
4 FAK001A Aperture, RBC/Plt, 50 µ
5 GBG156A O-ring, aperture, package of 12
6 GBG004A Collar
7 GBG157A Counting head (internal electrode)
10 FAA066A O-ring, bath drain/debubble chamber, package of 12
11 GBG007A Diffuser, drain and debubble, set of 4
12 GBG003A Chamber, drain and debubble
8.2-46 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-47 Hgb Photometer Assembly (See Table 8.2-47)
LML220AC
Table 8.2-47 Hgb Photometer Assembly (See Figure 8.2-47)

1 XDA610A Bath, WBC/BASO
3 XDA602A Bath, assembly, 3-baths and rinse chamber
4 XBA389A Photometer, Hgb diode and preamp assembly
PN 4237616B 8.2-47
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-48 WBC/BASO Bath Assembly (See Table 8.2-48)
LML225AC
Table 8.2-48 WBC/BASO Bath Assembly (See Figure 8.2-48)

2 XBA398B Cable, coaxial, with RBC/WBC bath electrode
3 GBG002A Bath, WBC/Hgb/BASO
4 FAK003A Aperture, WBC/BASO, 80 µm
5 GBG156A O-ring, aperture, package of 12
6 GBG004A Collar
7 GBG157A Counting head (internal electrode)
10 FAA066A O-ring, bath drain/debubble chamber, package of 12
11 GBG007A Diffuser, drain and debubble, set of 4
12 GBG003A Chamber, drain and debubble
8.2-48 PN 4237616B
PARTS LISTS
ILLUSTRATED PARTS 8
Figure 8.2-49 Rear Frame Assembly (See Table 8.2-49)
LML903AC
Table 8.2-49 Rear Frame Assembly (Figure 8.2-49)

1 Frame assembly
2 DBK021A Rivets
3 KAH018A Nut, M4
4 KAJ002A Washer, M diameter 0.4
5 FAN001A Fan protector
6 XBA393A Fan, main chassis, 24V
8 GBG058A Plate, rear frame
10 EAC010A Fitting, Luer, female, i.d. 3 mm, package of 5
11 EAC008A Fitting, antirotation washer, package of 5
12 GBG071A Receptacle
13 KAH024A Nut 1/4-28 (US std)
14 XDA623AS Panel, reagent/waste fittings assembly
PN 4237616B 8.2-49
PARTS LISTS
ILLUSTRATED PARTS
8.2-50 PN 4237616B
CONTENTS
A QUICK REFERENCE INFORMATION, A.1-1
A.1 TOLERANCE AND LIMITS, A.1-1
A.2 CIRCUIT CARD LAYOUTS WITH KEY COMPONENT DESCRIPTIONS, A.2-1

Main Card, A.2-1
Test Points, A.2-2
Potentiometers, A.2-4
Jumper Settings, A.2-5
Optical Preamplifier Card, A.2-6
Connectors, A.2-6
LCD and Keypad Card, A.2-7
LED Card, A.2-8
Connectors, A.2-8
Motor Interconnect Card, A.2-9
Connectors, A.2-9
Traverse Card, A.2-10
Connectors, A.2-10
A.3 AC•T 5diff MODULE LOCATIONS AND FUNCTIONS, A.3-1

Overview, A.3-1
Analyzer Modules, A.3-1
Mechanical and Hydraulic Modules Locations, A.3-2
Rear Panel, A.3-4
A.4 SOFTWARE MENU TREE, A.4-1
B SOFTWARE INTERFACE, B.1-1
B.1 FORMAT , B.1-1
B.2 PACKET TYPE PIN ASSIGNMENTS , B.2-1
C FLAG SENSITIVITY AND THRESHOLDS, C.1-1
C.1 OVERVIEW, C.1-1

Flag Sensitivity, C.1-1
Thresholds, C.1-2
Plt Threshold, C.1-2
WBC and BASO Thresholds, C.1-2
DiffPlot Thresholds, C.1-3
DiffPlot - Volume Thresholds, C.1-4
DiffPlot - Absorbance Thresholds, C.1-6
NL, NE, and MN Alarms, C.1-7
RBC Histogram, C.1-7
PN 4237616B 9-i
CONTENTS
C.2 SETTING FLAG SENSITIVITY , C.2-1

Purpose, C.2-1
Procedure, C.2-1
Verification, C.2-1
C.3 SETTING THRESHOLDS , C.3-1

Purpose, C.3-1
Procedure, C.3-1
Verification, C.3-2
ILLUSTRATIONS
A.2-1 Main Card Components, A.2-1
A.2-2 Main Card Jumper Settings, A.2-5
A.2-3 Optical Preamplifier Card Components, A.2-6
A.2-4 Keypad and LCD Card Components, A.2-7
A.2-5 LED Card Components, A.2-8
A.2-6 Motor Interconnect Card Components, A.2-9
A.2-7 Traverse Card Components, A.2-10
A.3-1 View of an AC•T 5diff Hematology Analyzer with the Right Side Door
Open, A.3-2
A.3-2 View of an AC•T 5diff Hematology Analyzer with the Left Side Panel
Removed, A.3-2
A.3-3 Rear Panel - AC•T 5diff Hematology Analyzer, A.3-4
A.4-1 Software Menu Tree , A.4-1
C.1-1 PLT Threshold, C.1-2
C.1-2 WBC and BASO Threshold, C.1-2
C.1-3 DiffPlot, C.1-3
C.1-4 DiffPlot - Volume Thresholds (Y-axis), C.1-4
C.2-1 Flags Sensitivity Screen, C.2-1
C.3-1 Thresholds Screen, C.3-1
TABLES
A.1-1 Flow Cell Adjustment Limits, A.1-1
A.1-2 Motor Voltage Limits, A.1-1
A.1-3 Thresholds Voltage Limits, A.1-1
A.1-4 Mixing Bubble Limits, A.1-1
A.1-5 Power Supply Voltages, A.1-2
A.1-6 Whole-Blood Reproducibility CV Limits for 20 Cycles, A.1-2
A.1-7 Calibration Factor Limits, A.1-2
A.2-1 Main Card Test Points , A.2-2
A.2-2 Main Card Potentiometers, A.2-4
A.2-3 Connectors on the Optical Preamplifier Card, A.2-6
A.2-4 Connectors on the Keypad and LCD Card, A.2-7
A.2-5 Connector on the LED Card, A.2-8
A.2-6 Connectors on the Motor Interconnect Card, A.2-9
A.2-7 Connectors on the Traverse Card, A.2-10
9-ii PN 4237616B
CONTENTS
A.3-1 Mechanical and Hydraulic Modules, A.3-3

C.1-1 Flag Sensitivity Default Values, C.1-1
C.1-2 WBC/BASO Factory-Set Threshold Values, C.1-3
C.1-3 DiffPlot - Volume Thresholds (Y-Axis), C.1-5
C.1-5 DiffPlot - FNL, FNE, and FMN Thresholds, C.1-7
PN 4237616B 9-iii
CONTENTS
9-iv PN 4237616B
AQUICK REFERENCE INFORMATION A
A.1 TOLERANCE AND LIMITS
Table A.1-1 Flow Cell Adjustment Limits
Parameter Target Value Acceptable Range

DIFF LAMP 6.00 5.50 to 6.50
TRANSFER TIME 200 150 to 250
RESISTIVE CHANNEL 50 45 to 55
ABSORBANCE CHANNEL 180 170 to 190
Table A.1-2 Motor Voltage Limits
Motor Test Point Voltage Potentiometer

Waste syringe TP5 4 V ± 0.05 V R149
Count syringe TP6 4 V ± 0.05 V R150
Dilutor syringe TP7 4 V ± 0.05 V R151
Optical bench injector syringe TP8 3 V ± 0.05 V R152
Horizontal traverse TP10 3 V ± 0.05 V R154
Sample syringe TP11 2 V ± 0.05 V R155
Probe carriage TP12 5 V ± 0.05 V R156
Table A.1-3 Thresholds Voltage Limits
Threshold Test Point Voltage Potentiometer

BASO TP14 300 mV ± 5 R157
RBC TP13 300 mV ± 5 R158
PLT TP2 300 mV ± 5 R159
LMNE (DIFF) CIS TP3 650 mV ± 5 R160
LMNE (DIFF) OD TP4 350 mV ± 5 R161
Table A.1-4 Mixing Bubble Limits
Mixing Bath Low Limit Normal High Limit

FIRST DILUTION 100 300 400
DIFF 100 300 400
WBC/BASO 100 300 400
HGB LYSE 300 400 500
PN 4237616B A.1-1
QUICK REFERENCE INFORMATION
TOLERANCE AND LIMITS
Table A.1-5 Power Supply Voltages
Test Point Designation Target Value

TP40 5V Power supply +5V
TP43 -12V Power supply -12V
Table A.1-6 Whole-Blood Reproducibility CV Limits for 20 Cycles
Parameters %CV Test Level

WBC <2% at 10.0 x 103 cells / µL
RBC <2% at 5.00 x 106 cells / µL
Hgb <1% at 15.0 g/dL
Hct <2% at 45.0%
MCV <1% at 90.0 fL
Plt <5% at 300 x 103 cells / µL
Table A.1-7 Calibration Factor Limits
Minimum Maximum
Parameter Target Value Acceptable Value Acceptable Value
WBC 137 90 200
RBC 225 160 290
Hgb 40.0 25.0 55.0
Hct 220 160 290
PLT 290 180 400
RDW 0.3 0.1 0.9
A.1-2 PN 4237616B
CIRCUIT CARD LAYOUTS WITH KEY COMPONENT DESCRIPTIONS A
A.2 CIRCUIT CARD LAYOUTS WITH KEY COMPONENT DESCRIPTIONS
Main Card
Component Locations
Figure A.2-1 Main Card Components
PN 4237616B A.2-1
CIRCUIT CARD LAYOUTS WITH KEY COMPONENT DESCRIPTIONS
Test Points
Table A.2-1 Main Card Test Points
Test Point Designation Potentiometer Target Tolerance Remarks

TP1 HB Gain adjustment R248 4.7 mV ±0.1 mV
TP2 PLT Threshold adjustment R159 300 mV ±5 mV
TP3 LMNE (DIFF) CIS Threshold adjustment R160 650 mV ±5 mV
TP4 LMNE (DIFF) OD Threshold adjustment R161 350 mV ±5 mV
TP5 Waste syringe motor R149 3V ±50mV
TP6 Count syringe motor R150 3V ±50mV
TP7 Reagent syringes motor R151 3V ±50mV
TP8 Injection syringe motor R152 3V ±50mV
TP9 Not used
TP10 Horizontal traverse motor R154 3V ±50mV
TP11 Sample syringe motor R155 2V ±50mV
TP12 Vertical traverse motor R156 3V ±50mV
TP13 RBC Threshold adjustment R158 300 mV ±5mV
TP14 BASO Threshold adjustment R157 300 mV ±5mV
TP15 BASO Comparator
TP16 RBC Comparator
TP17 PLT Comparator
TP18 LMNE (DIFF) CIS Comparator
TP19 LMNE (DIFF) OD Comparator
TP20 RBC line height adjustment R133 XX V ±50 mV Factory adjusted
TP21 BASO gain adjustment R134 XX V ±50 mV Factory adjusted
TP22 PLT line height adjustment R135 XX V ±50 mV Factory adjusted
TP23 DIFF Resistive gain adjustment R136 XX V ±50 mV Factory adjusted
TP24 DIFF Optical gain adjustment R148 XX V ±50 mV Factory adjusted
TP25 RBC line width adjustment
TP26 RBC line reject adjustment R142 10 µs ±0.5 µs Factory adjusted
TP27 RBC line pulse adjustment
TP28 BASO line width adjustment
TP29 BASO line reject adjustment R139 10 µs ±0.5 µs Factory adjusted
TP30 BASO line pulse adjustment
TP31 PLT line width adjustment
TP32 PLT line reject adjustment R144 10 µs ±0.5 µs Factory adjusted
TP33 PLT line pulse adjustment
TP34 DIFF Resistive line width adjustment
A.2-2 PN 4237616B
Table A.2-1 Main Card Test Points (Continued)
Test Point Designation Potentiometer Target Tolerance Remarks

TP35 DIFF Resistive line reject adjustment R145 15 µs ±0.5 µs Factory adjusted
TP36 DIFF Resistive line pulse adjustment
TP37 DIFF Optical line width adjustment
TP38 DIFF Optical line reject adjustment R147 5 µs ±0.5 µs Factory adjusted
TP39 DIFF Optical line pulse adjustment
TP40 5V Power supply 5V
TP41 12V Power supply 12 V
TP42 24V Power supply 24 V
TP43 -12V Power supply -12 V
TP44 GROUND
TP45 GROUND
TP46 GROUND
TP47 GROUND
TP48 Waste sensor R286 <1 V With water
TP49 Not used
TP50 Reagent heating system temperature
voltage
TP51 Thermostated room heating system
temperature voltage
TP52 DIFF drain sensor R287 <1 V With water
TP53 DIFF Lamp R11 6V
TP54 GROUND
TP55 GROUND
TP56 GROUND
TP57 GROUND
TP58 GROUND
TP59 GROUND
TP60 DIFF Resistive line reject2 adjustment R409 50µs ±2 µs Factory adjusted
TP61 DIFF Resistive line reject2 adjustment R410 250µs ±5 µs Factory adjusted
PN 4237616B A.2-3
Potentiometers
Table A.2-2 Main Card Potentiometers
Potentiometer Designation Test Point Target Tolerance Remarks

R133 RBC line height adjustment TP20 Factory adjusted
R134 BASO gain adjustment TP21 Factory adjusted
R135 PLT line height adjustment TP22 Factory adjusted
R136 DIFF Resistive gain adjustment TP23 Factory adjusted
R139 BASO line reject adjustment TP29 Factory adjusted
R142 RBC line reject adjustment TP26 Factory adjusted
R144 PLT line reject adjustment TP32 Factory adjusted
R145 DIFF Resistive line reject TP35 Factory adjusted
adjustment
R147 DIFF Optical line reject adjustment TP38 Factory adjusted
R148 DIFF Optical gain adjustment TP24 Factory adjusted
R149 Waste syringe motor TP5 3V ±50 mV
R150 Count syringe motor TP6 3V ±50 mV
R151 Reagent syringes motor TP7 3V ±50 mV
R152 Injection syringe motor TP8 3V ±50 mV
R154 Horizontal traverse motor TP10 3V ±50 mV
R155 Sample syringe motor TP11 2V ±50 mV
R156 Vertical traverse motor TP12 3V ±50 mV
R157 BASO Threshold adjustment TP14 300 mV ±5 mV
R158 RBC Threshold adjustment TP13 300 mV ±5 mV
R159 PLT Threshold adjustment TP2 300 mV ±5 mV
R160 LMNE (DIFF) CIS Threshold TP3 650 mV ±5 mV
adjustment
R161 LMNE (DIFF) OD Threshold TP4 350 mV ±5 mV
adjustment
R248 HB Gain adjustment TP1 4.7 mV ±0.1 mV
A.2-4 PN 4237616B
Jumper Settings
Figure A.2-2 Main Card Jumper Settings
Note: If you have an initialization problem with the 68HC11 Microcontrolers, remove the
E20 jumper to reset AC•T 5diff analyzer. (The instrument must be turned ON).
PN 4237616B A.2-5
Optical Preamplifier Card
Component Locations
Figure A.2-3 Optical Preamplifier Card Components
Connectors
Table A.2-3 Connectors on the Optical Preamplifier Card
Card Label Description

J1 Optical output signal
J2 Power to card
A.2-6 PN 4237616B
LCD and Keypad Card
Component Locations
Figure A.2-4 Keypad and LCD Card Components
Connectors
Table A.2-4 Connectors on the Keypad and LCD Card

J1 Main interface to main card
J2 Backlight power to LCD
J3 Data out to LCD
J4 Power to aspirate indicator card
PN 4237616B A.2-7
LED Card
Component Locations
Figure A.2-5 LED Card Components
Connectors
Table A.2-5 Connector on the LED Card

J1 Power/signal to LEDs
A.2-8 PN 4237616B
Motor Interconnect Card
Component Locations
Figure A.2-6 Motor Interconnect Card Components
Connectors
Table A.2-6 Connectors on the Motor Interconnect Card

J1 Traverse horizontal home sensor
J2 Power/signals from Main card
J4 Float sensor
J5 Injector syringe home switch
J6 Reagent syringe home switch
J7 Count syringe home switch
J8 Waste syringe home switch
J9 Not used
J10 Injector syringe motor
J11 Reagent syringe motor
J12 Count syringe motor
J13 Waste syringe motor
J14 Traverse horizontal motor
PN 4237616B A.2-9
Traverse Card
Component Locations
Figure A.2-7 Traverse Card Components
Connectors
Table A.2-7 Connectors on the Traverse Card

J1 Traverse vertical motor
J2 Sample syringe home switch
J3 Traverse vertical home sensor
J4 Sample syringe motor
J5 Power/interface to Main card
A.2-10 PN 4237616B
AC•T 5diff MODULE LOCATIONS AND FUNCTIONS A
A.3 AC•T 5diff MODULE LOCATIONS AND FUNCTIONS
Overview
Most functions are accomplished by fluidic components that are interconnected by tubing
and controlled by timed solenoid signals. This section briefly describes the functions of these
fluidic components and shows their locations.
Analyzer Modules
The AC•T 5diff hematology analyzer consists of nine mechanical and hydraulic modules.
These modules are identified on Table A.3-1. A description of the module’s primary functions
is also included. Figures A.3-1 and A.3-2 show each module’s location inside the instrument.
PN 4237616B A.3-1
AC•T 5diff MODULE LOCATIONS AND FUNCTIONS
Mechanical and Hydraulic Modules Locations

Figure A.3-1 View of an AC•T 5diff Hematology Analyzer with the Right Side Door Open
Figure A.3-2 View of an AC•T 5diff Hematology Analyzer with the Left Side Panel Removed
A.3-2 PN 4237616B
AC•T 5diff MODULE LOCATIONS AND FUNCTIONS A
Table A.3-1 Mechanical and Hydraulic Modules
Figure
Reference Module Functions
Figure A.3-1, 1 Traverse Assembly r Ensures sample probe positioning for the different sample stages
and distribution.
r Supports the sample syringe and the distribution of the blood.
Figure A.3-1, 2 Sample Syringe Assembly r Takes the sample and distributes the portions into the different
baths.
r Removes a sample from the first dilution (made in the DIL1/HGB
bath) and dispenses it into the RBC bath.
Figure A.3-1, 3 Baths Assembly inside the r Receives the different rinsing and dilutions.
Bath Enclosure r Ensures the temperature control of the dilutions.
r Ensures the counts for WBC, BASO, RBC, Plt, and Hct.
r Ensures Hgb determination.
Figure A.3-1, 4 Waste Syringe r Drains the different baths.
r Provides mixing bubbles to the mixtures.
r Provides the vacuum needed to pull the DIFF specimen from the
DIFF bath towards the flow cell injector.
Figure A.3-2, 5 Diluent Reservoir r Holds the diluent needed for an analysis cycle.
r Eliminates the risk of diluent degassing as it is being aspirated by
the syringes.
Note: Reservoir is vacuum filled by the count syringe.
Figure A.3-1, 6 Count Syringe r Provides the vacuum needed for the WBC and the BASO counts.
r Provides the vacuum needed for the RBC and PLT counts.
r Provides the vacuum needed for filling the diluent reservoir.
Figure A.3-2, 7 5diff Syringe Assembly r Ensures correct proportioning of the stop diluent in the DIFF
preparation bath.
r Injects the sample into the flow cell.
r Injects the interior and exterior gains into the flow cell.
Figure A.3-2, 8 Reagent Syringes Assembly r Ensures the correct distribution of the different reagents including:
t Lysing agent for hemoglobin (AC•T 5diff Hgb Lyse).
t Cleaning reagent (AC•T 5diff Rinse).
t Lysing agent for the DIFF (AC•T 5diff Fix).
t Diluent used for the dilutions (AC•T 5diff Diluent) except the DIFF
stop diluent.
t Lysing agent for WBC/BASO (AC•T 5diff WBC Lyse).
Figure A.3-2, 9 Optical Bench Assembly r Provides support and ensures adjustment of the flow cell.
r Provides support and ensures adjustment of the optics lamp.
r Provides support and ensures adjustment of the optical and
electronic elements.
PN 4237616B A.3-3
AC•T 5diff MODULE LOCATIONS AND FUNCTIONS
Rear Panel
Figure A.3-3 Rear Panel - AC•T 5diff Hematology Analyzer
Figure Reference Attachments to the Rear Panel

Figure A.3-3, 1 Waste output
Figure A.3-3, 2 Diluent input
Figure A.3-3, 3 Serial number label
Figure A.3-3, 4 Bar-code reader connector
Figure A.3-3, 5 Printer connector
Figure A.3-3, 6 RS232C output
Figure A.3-3, 7 Power cord
A.3-4 PN 4237616B
SOFTWARE MENU TREE A
A.4 SOFTWARE MENU TREE
Figure A.4-1 Software Menu Tree
MAIN MENU
CALIBRATION
1 - RUN SAMPLES
2 - CALIBRATION 1 - AUTOCALIBRATION
3 - REAGENTS 2 - CAL FACTORS
4 - DIAGNOSTICS 3 - PRINT CAL FACTORS
5 - SETUP 4 - REPRODUCIBILITY RINSE
PRIME 1 - BATHS
REAGENTS 2 - FLOWCELL
1 - LEVEL-CHANGE 1- DILUENT
2 - DAILY WORKLOAD 2 - FIX DRAIN BATHS
3 - PRIME 3 - WBC LYSE
4 - HGB LYSE 1 - RINSE
5 - RINSE 2 - FIRST DILUTION
6 - ALL REAGENTS 3 - DIFF
7 - UNPRIME ALL 4 - WBC / BASO
5 - RBC / PLT
6 - ALL BATHS
7 - DILLUENT RESERVOIR
DIAGNOSTICS DILUTER SYSTEMS
1-SYSTEM RESET CYCLE
2-MINI PRIME 1-BACKFLUSH
2-RINSE MOTORS
3-DILUTER SYSTEMS
4-HARDWARE SYSTEMS 3-DRAIN BATHS
4-EXTENDED CLEANING 1 - SAMPLING PROBE
5-SERVICE 2 - TRAVERSE
3 - SAMPLING SYRINGE
4 - DRAINING SYRINGE
HARDWARE SYSTEMS 5 - COUNTING SYRINGE
6 - FLOWCELL SYRINGES
1-HARDWARE RESET 7 - DILUTION SYRINGES
2-MOTORS *
3-VALVES *
4-TRAVERSE SERVICE POSITION VALVES
1 - 1 TO 11
SERVICE 2 - 12 TO 16
PASSWORD 3 - 17 AND 18
REQUIRED 4 - 20 TO 26 MEASURMENT
5 - 27 TO 31
SERVICE 1 - HGB BLANK ADJUSTMENT
1 - DILUTION 2 - APERTURE CURRENT
2 - MEASUREMENT 3 - RBC / PLT GAIN
3 - HEATING SYSTEMS 4 - WBC / BASO GAIN
4 - MIXING 5 - DIFF ADJUSTMENT
5 - SENSOR CHECK 6 - PULSE ADJUSTMENT
6 - VACUUM CHECK
7 - BURN-IN
8 - FLOWCELL WBC CALIBRATION HEATING SYSTEMS
9 - OTHERS
1 - HEATING COIL
2 - BATH ENCLOSURE
SET DATE / TIME
SETUP 1 - TIME FORMAT SENSOR CHECK
2 - DATE FORMAT
1 - DATE / TIME 3 - SET DATE & TIME 1 - DRAINING
2 - UNITS 2 - DIFF TRANSFER HEATING COIL
3 - LAB. LIMITS PATIENT RANGES
4 - HOST SETUP LAB. LIMITS 1 - ADJUSTMENT
5 - PRINTER 1 - CBC 2 - REFERENCE
6 - OTHERS 1 - PATIENT RANGES 2 - DIFF VACUUM CHECK
2 - ACTION RANGES
3 - FLAGS SENSITIVITY 1 - COUNTING BATH ENCLOSURE
4 - THRESHOLDS ACTION RANGES 2 - DRAINING
1 - ADJUSTMENT
1 - CBC 2 - REFERENCE
HOST SETUP BURN - IN
2 - DIFF
1 - HOST SETUP CONFIGURATION 1 - BURN - IN CYCLES
2 - SENDING CONFIGURATION 2 - ANALYSIS CYCLES
3 - SENDING OPTIONS
4 - VARIABLE FORMAT SETUP VARIABLE FORMAT SETUP
FLOWCELL WBC CALIBRATION
5 - SEND LATEST RESULT
1 - NUMERICAL RESULTS
2 - FLAGS AND MESSAGES 1 - AUTOCALIBRATION
3 - HISTOGRAMS AND THRESHOLDS 2 - CAL FACTORS
PRINTER
4 - PATIENT FILE
1 - PRINTER CONFIGURATION OTHERS
* Consult a Beckman Coulter 2 - INSTITUTIONNAL HEADER
representative before selecting this 3 - PRINT LATEST RESULT 1 - USER MODE
option. 2 - CYCLE COUNTS
CALIBRATION 3 - PARK SYRINGES
Service password required 4 - RESET TO DEFAULT VALUES
OTHERS 1 - CV% L I M I TS
2 - DEFINE OPERATOR
1 - CALIBRATION
2 - IDENTIFICATION MODE
3 - AUTOCLEAN FREQUENCY LANGUAGE
4 - CHANGE PASSWORD
5 - LANGUAGE 1 - ENGLISH
6 - REAGENT VOLUMES 2 - FRENCH
7 - CYCLE COUNTS 3 - GERMAN
8 - PRINT SYSTEM SETUP 4 - SPANISH 7616096B
9 - SEND SETUP 5 - ITALIAN
PN 4237616B A.4-1
SOFTWARE MENU TREE
A.4-2 A.4-2 PN 4237616B

BSOFTWARE INTERFACE B
B.1 FORMAT
See Host Transmission Specification PN 4277065A.
PN 4237616B B.1-1
SOFTWARE INTERFACE
FORMAT
B.1-2 PN 4237616B
SOFTWARE INTERFACE
PACKET TYPE PIN ASSIGNMENTS B
B.2 PACKET TYPE PIN ASSIGNMENTS
COMPUTER SIDE
DB9
RS 232 OUTPUT CONFIGURATION
GND GND 5
5
6 6
4 4
7 TXD TXD 3 7
3
8 RXD RXD 2 8
2
9 9
1 1
COMPUTER SIDE
DB25
CONFIGURATION
13
RS 232 OUTPUT
GND GND 7
5
6 6
4
7 TXD TXD 3 7
3
8 RXD RXD 2 8
2
9 9
1 1
7616033A
PN 4237616B B.2-1
SOFTWARE INTERFACE
PACKET TYPE PIN ASSIGNMENTS
B.2-2 PN 4237616B
CFLAG SENSITIVITY AND THRESHOLDS C
C.1 OVERVIEW
The instrument flags a sample if its results exceed specific criteria defined within the
software.
The values used to position the thresholds, which separate different cell populations and
determine if a flag should be triggered, are selected to provide optimal population separation
and flagging under normal operating conditions.
The software provides you with the ability to modify the values used to position the
thresholds between populations and to alter the level at which a flag associated with a
population is triggered.
Changing any of the values that define threshold positions or changing the flag sensitivity
values from the default values will alter the parameter values reported and whether or not a
flag is associated with a result.
Flag Sensitivity
A result outside the flag sensitivity range triggers a flag to appear with the parameter result.
The flag occurs if either the percentage or absolute count value is exceeded.
You can adjust a flag’s sensitivity for percentage and/or absolute number. Table C.1-1 shows
the flag sensitivity default values.
Table C.1-1 Flag Sensitivity Default Values
Flag Region Percentage of Cells Absolute Number of Cells

DB (Debris) 100 120
SL (Small Lymphocytes) 100 50
SL1 (Small Lymphocytes 1) 5 45
NL (Neutrophil / Lymphocyte) 3 120
MN (Monocyte / Neutrophil) 100 120
UM (Upper Monocyte) 1.1 999
LN (Lower Neutrophil) 2.5 999
UN (Upper Neutrophil) 1.1 999
NE (Neutrophil / Eosinophil) 1.1 60
ATL (Atypical Lymphocytes) 2 200
*WBC 3.5 999
MICRO (Microcytes) 5* Not applicable
MACRO (Macrocytes) 7.5* Not applicable
Hgb M 3.0% Not applicable
Hgb B Not applicable 60 (A to D units)
Note: Hgb M - defines the allowable difference between the three readings taken on the sample.
Hgb B - defines the allowable difference between the reference blank and sample blank.
* MICRO and MACRO flags are activated in software version 1.03 and higher.
PN 4237616B C.1-1
FLAG SENSITIVITY AND THRESHOLDS
OVERVIEW
r For a definition of each flag, see Chapter 6 of the Operator’s Guide.

r If you need to change a flag sensitivity value, use the procedure under Heading C.2.
Thresholds
The values used to position the thresholds, which separate different cell populations and
determine if a flag should be triggered, are selected to provide optimal population separation
and flagging under normal operating conditions.
Plt Threshold
The PLT threshold (PLT 1) is the number of the last channel used to calculate the platelet
count (Figure C.1-1). The factory setting for the Plt threshold is 197.
Figure C.1-1 PLT Threshold
If you must change the platelet threshold, use the procedure under Heading C.3. However, be
aware that changing this value from the default value will alter parameter values reported and
whether or not those results are flagged.
WBC and BASO Thresholds

Differentiation between basophils and other leukocytes is obtained by means of the
AC•T 5diff WBC Lyse-specific lytic action. Thresholds BA1, BA2, and BA3 allow the
determination of the basophil population in relationship to the total number of white blood
cells (Figure C.1-2).
Figure C.1-2 WBC and BASO Threshold
C.1-2 PN 4237616B
OVERVIEW C
In Figure C.1-2, all leukocytes are shown between the BA1 and BA3 thresholds. *WBC
absolute value is calculated between channel 0 and the BA1 threshold.
The percentage of basophils is calculated according to the number of particles from the BA2
threshold to the BA3 threshold. These thresholds are factory-set to the values shown in
Table C.1-2.
Table C.1-2 WBC/BASO Factory-Set Threshold Values
Threshold Purpose Channel

0 *WBC # counting area 0
BA1 Separation threshold between the *WBC # counting area and the WBC 35
BA2 Separation threshold between the WBC and basophils 110
BA3 End of the basophil counting area 240
If you must change the BA1, BA2, and BA3 thresholds, use the procedure under Heading C.3.
However, be aware that changing these values from the default values will alter the parameter
values reported and whether or not those results are flagged.
DiffPlot Thresholds
On the DiffPlot, both the X-axis and Y-axis are divided into 128 channels, numbered from 0
to 127 (Figure C.1-3).
Figure C.1-3 DiffPlot
Although invisible, there are 13 vertical grids on the DiffPlot’s X-axis and 13 horizontal grids
on the Y-axis. These grids form channels that can be located and given numbers. The origin or
first location is Channel 0, at the bottom left corner. The fourth location of the DiffPlot is
channel 30, and so forth. Threshold adjustment is expressed in channels.
PN 4237616B C.1-3
OVERVIEW
Adjustment of the thresholds may be considered to:

r Improve the separation between different cell populations that may vary according to the
anti-coagulant used or the instrument’s internal adjustment.
r Modify the flag areas to improve detection sensitivity. You must also readjust the value of
the respective flags.
r Modify one or several DiffPlot regions to precisely define a specific population for
research purposes.
There are three categories of threshold adjustment limits:

r Volume thresholds on the Y-axis.
r Absorbance thresholds on the X-axis.
r FNL, FNE, FMN thresholds which indicate the width of the channel in the NL, NE, and
MN alarm areas.
DiffPlot - Volume Thresholds

The volume thresholds on the Y-axis are identified in Figure C.1-4 and explained in
Table C.1-3.
Figure C.1-4 DiffPlot - Volume Thresholds (Y-axis)
C.1-4 PN 4237616B
OVERVIEW C
Table C.1-3 DiffPlot - Volume Thresholds (Y-Axis)
Default
Threshold Purpose Channel Low Limit High Limit
DL Separates debris and small lymphocytes. 22 0 SL
DN Separates debris and lower neutrophils. 25 DL DE
SL Separate lower lymphocytes and lymphocytes. 30 NL AL
LN Separates neutrophils and small neutrophils. 35 DN LMN
DE Separates debris and eosinophils. 48 DN Channel 127
LMN Intersection point between the LY, MO, and NE 70 LN LMU
thresholds.
AL Separates lymphocytes and atypical lymphocytes. 68 SL LMU
LMU Lower dot on the separation slope between atypical 78 AL LMD
lymphocytes and monocytes.
LMD Upper dot on the separation slope between atypical 90 LMU UM
lymphocytes and monocytes.
MN Upper dot on the separation slope between 90 LMN UM
monocytes and neutrophils.
UM Separates monocytes and upper monocytes. 118 LMD Channel 127
UN Separates neutrophils and upper neutrophils. 118 MN Channel 127
PN 4237616B C.1-5
OVERVIEW
DiffPlot - Absorbance Thresholds

The absorbance thresholds on the X-axis are identified in Figure C.1-5 and explained in
Table C.1-4.
Figure C.1-5 DiffPlot - Absorbance Thresholds (X-Axis)
Table C.1-4 DiffPlot - Absorbance Thresholds (X-Axis)
Default
Thresholds Purpose Channel Low Limit High Limit
NL Separates lymphocytes and neutrophils. 29 0 RMN
RMN Separates upper monocytes and upper 51 NL NE
neutrophils.
NE Separates neutrophils and eosinophils. 78 RMN Channel 127
C.1-6 PN 4237616B
OVERVIEW C
NL, NE, and MN Alarms
FNL, FNE, FMN thresholds indicate the width in channel of the NL, NE, and MN alarm
areas. The alarms are identified in Figure C.1-5 and explained in Table C.1-5.
Table C.1-5 DiffPlot - FNL, FNE, and FMN Thresholds
Threshold Purpose Default Channel

FNL Channel number for the NL alarm region. 2
FNE Channel number for the NE alarm region. 2
FMN Channel number for the MN alarm region. 2
RBC Histogram
RDI (Red Cell Distribution Index) threshold indicates the width in channel numbers of the
RBC1 and RBC2 thresholds used in MICRO and MACRO calculation. The default value is
12.2.
PN 4237616B C.1-7
OVERVIEW
C.1-8 PN 4237616B
SETTING FLAG SENSITIVITY C
C.2 SETTING FLAG SENSITIVITY
Purpose
IMPORTANT Risk of inaccurate flagging. Use extreme caution when doing this procedure, flag sensitivity
settings are typically not changed from the factory default settings.
It is strongly recommended that no changes be made to the default values. Changing the flag
sensitivity values from the default values will alter whether or not a flag will be associated
with a result.
Use this procedure only if changing the flag sensitivity values is absolutely necessary. You may
also use this procedure to re-enter the flag sensitivity default values listed in Table C.1-1.
B Service password (If needed, see Service Password under Heading 4.1.)
Procedure
1. From the Main menu, select SETUP tt LAB. LIMITS tt FLAGS SENSITIVITY. The SERVICE
PASSWORD prompt appears.
2. At the instrument keypad, press the numeric keys correlating to the Service password
then press ENTER. The Flags Sensitivity screen appears (Figure C.2-1).
Figure C.2-1 Flags Sensitivity Screen
FLAGS SENSITIVITY 12 / 07 / 99 16:05

FLAGS SENSITIVITY 1
DIFF REJECT 50.0
DB % 100.0 # 120 *WBC% 3.5 # 999
SL % 100.0 # 50
SL1 % 5.0 # 45 MICRO 5.0
NL % 3.0 # 120 MACRO 20.0
MN % 100.0 # 120
UM % 1.1 # 999 HGBM% 3.0
LN % 2.5 # 999 HGBB# 60
UN % 1.1 # 999
NE % 1.1 # 60
7616127B
3. Move the cursor to the value to be changed.

4. Edit the value using the numeric keypad.
5. Press ENTER to accept the new value and move to the next field.
6. Repeat steps 3 through 5 as needed to change additional values.
7. Select the next range and repeat steps 3 through 7 as required.
8. When all changes are completed, press ESC as many times as necessary to return to the
Main Menu.
Verification
Run several normal and abnormal whole-blood specimens to verify the results are accurate
with proper flagging, as applicable.
PN 4237616B C.2-1
SETTING FLAG SENSITIVITY
C.2-2 PN 4237616B
SETTING THRESHOLDS C
C.3 SETTING THRESHOLDS
Purpose
IMPORTANT Risk of inaccurate results. Use extreme caution when doing this procedure, threshold settings
are typically not changed from the factory default settings.
It is strongly recommended that no changes be made to the default values. By changing the
thresholds, you are altering the populations. Therefore, changing any of the values that define
threshold positions will ultimately alter the parameter values reported and whether or not a
flag is associated with a result.
Use this procedure only if changing the threshold values is absolutely necessary. You may also
use this procedure to re-enter the threshold default values listed in Appendix C:
r Table C.1-2, WBC/BASO Factory-Set Threshold Values
r Table C.1-3, DiffPlot - Volume Thresholds (Y-Axis)
r Table C.1-4, DiffPlot - Absorbance Thresholds (X-Axis)
r Table C.1-5, DiffPlot - FNL, FNE, and FMN Thresholds
B Service password (If needed, see Service Password under Heading 4.1.)
Procedure
1. From the Main menu, select SETUP tt LAB. LIMITS tt THRESHOLDS. The SERVICE
PASSWORD prompt appears.
2. At the instrument keypad, press the numeric keys correlating to the Service password
then press ENTER. The Thresholds screen appears (Figure C.3-1).
Figure C.3-1 Thresholds Screen
THRESHOLDS 12 / 07 / 99 16:05
THRESHOLDS 1
BA1 35 BA2 110 BA3 240

DL 22 DN 25 DE 48 LN 35
UN 118 SL 30 AL 68 LMU 68
LMD 90 LMN 70 MN 90 UM 118
NL 29 NE 78 UMN 51
FNL 2 FNE 2 FMN 2
RDI 12.2
7616128B
PN 4237616B C.3-1
SETTING THRESHOLDS
3. Move the cursor to the value to be changed.

4. Edit the value using the numeric keypad.
5. Press ENTER to accept the new value and move to the next field.
6. Repeat steps 3 through 5 as needed to change additional values.
7. Select the next range and repeat steps 3 through 7 as required.
8. When all changes are completed, press ESC as many times as necessary to return to the
Main Menu.
Verification
Run several normal and abnormal whole-blood specimens to verify the results are accurate
with proper flagging, as applicable.
C.3-2 PN 4237616B
CONTENTS
10
ABBREVIATIONS, ABBREVIATIONS-1
PN 4237616B 10-i
CONTENTS
10-ii PN 4237616B
ABBREVIATIONS
The following list is a composite of the abbreviations, acronyms and reference designators used in this
manual. When the same abbreviation (or reference designator) is used for more than one word (or type of
component), all meanings relevant to this manual are included, separated by semicolons.
SYMBOLS EV -
°C - degrees Celsius EOS - eosinophil
°F - degrees Fahrenheit
> - greater than F
≥ - greater than or equal to F - fuse
< - less than fL - femtoliters
≤ - less than or equal to ft - feet
µA - micro amperes
µL - microliters G
g - grams
µs - microseconds
g/dL - grams per deciliter
- - minus
% - percent
+ - plus
H
Hct - hematocrit
± - plus or minus
Hg - mercury
Hgb -hemoglobin
A
Hz - hertz
ac - alternating current
ATL - atypical lymphocytes
I
i.d. - internal diameter
B
in. - inches
BA - basophil
in. Hg - inches of mercury
I/O - input/output
C
cm - centimeters IMM - immature cell
CTN -
CV - coefficient of variant; check valve J
J - receptacle connector
D
dc - direct current K
K - kilos
DB - debris
KΩ - kilohms
Dil - diluent
KHz - kilohertz
DVM - digital voltmeter
DFF - dual focused flow
L
LCD - liquid crystal display
E
LED - light emitting diode
ESD - electrostatic discharge
PN 4237616B ABBREVIATIONS-1
ABBREVIATIONS
LN - lower neutrophil Q
LYMPH -lymphocyte QA - quality assurance
QC - quality control
M
MΩ - megohms R
M - motor R - resistor
mA - milli-amperes RAM - random access memory
max - maximum RBC - red blood cell count
MB - megabytes RDW - red cell distribution width
MACRO - macrocytes RF - reference
MCH - mean cell hemoglobin RH - relative humidity
MCHC - mean cell hemoglobin concentration RN - resistor
MCV - mean corpuscle volume ROM - read only memory
MICRO - microcytes RPWV - red pulse-width value
MN - monocyte/neutrophil) RS-232 - Electronic Industries Association standard
MONO - monocyte governing interface between data processing
and data communications equipment
MHz - megahertz
mb - millibars S
mL - milliliters S - switch; solenoid
mm - millimeters SLO-BLO - slow blow
mN.m - milliNewton meter SW - switch
SL - small lymphocytes
N SL1 - small lymphocytes 1
NE - neutrophil /eosinophil)
NEUT - neutrophil T
NL - neutrophil /lymphocyte Temp - temperature
nm - nanometers TP - test point
O U
o.d. - outside diameter UM - upper monocyte
ozf.in - ounce force inch UN - upper neutrophil
P V
P - plug connector V - volts
PC - printed circuit Vac - vacuum; volts alternating current
PCB - printed circuit board Vdc - volts direct current
Plt - platelet count
PN - part number W
ppm - pages per minute W - watt
2-ABBREVIATIONS PN 4237616B
ABBREVIATIONS
WBC - white blood cell count X

WM - wire marker X - jumper; plugged
WMCV - white mean corpuscle value x - times
WPWV - white pulse-width voltage
PN 4237616B ABBREVIATIONS-3
ABBREVIATIONS
4-ABBREVIATIONS PN 4237616B
INDEX
Numerics bar code reader cable, interconnect diagram, 6.5-9

5diff syringe, 4.29-1 BASO
5-part leukocyte differential, 2.1-1 calculation of BASO count, 2.7-1
+5V voltage, 4.20-4 count, 2.4-4
+12V voltage, 4.20-4 thresholds, histogramic illustration, 2.2-5
-12V voltage, 4.20-4 basophils, 2.1-1, 2.3-3, 2.4-6
+24V voltage, 4.20-4 bath diluent reagent circuit, illustration of, 2.8-14
35°C (95°F), regulated temperature of, 2.2-5 bath dilutions, volumes and ratios
DIFF bath, 2.3-3, 2.4-7
DIL1/HGB bath, 2.3-5, 2.4-2, 2.4-7
A RBC bath, 2.3-5, 2.4-2, 2.4-7
abbreviations WBC/BASO bath, 2.3-3, 2.4-7
used in this manual, ABBREVIATIONS-1 bath drain and DIFF transfer sensor, interconnect
absolute number of cells, C.1-1 diagram, 6.5-4
absorb light, ability to, 2.2-3 bath enclosure
absorbance cytochemistry, 2.2-4 cleaning, 4.28-1
AC•T 5diff hematology analyzer temperature, 4.16-1
general operation, 2.1-1 baths assembly, 2.2-6
menu system, 2.1-2 Biohazards Safety Guide, 1.2-1
purpose and function, 2.1-1 biological hazards, 1.2-1
accessories, table of, 8.1-6 blast cells, 2.7-4
ACV Technology, 2.2-1 bleach solution, bath enclosure cleaning, 4.28-1
adjustments. See checks and adjustments See also bubble limits
AL threshold, 2.7-5 bubble limits, 4.18-1, A.1-1
alignment, baths assembly, 4.5-1
alignment, counting bath, 4.35-8
altitude range, 3.1-1
C
ambient temperature, 3.1-1 calibration, 5.3-2
anti-extrusion of startup results, 3.2-11
ring, 4.25-5, 4.25-6, 4.25-7, 4.27-5, 4.27-6, 4 tolerances and limits, A.1-2
.27-7 calibration factor, 4.38-1
aperture current, 4.8-1 CAUTION
aperture diameter, measurement definition, 1.1-3
characteristics, 2.4-2 risk of damage to electronic
aperture sensor technology, 2.2-1 components, 1.2-1, 4.1-1
aspirate switch, 2.1-1, 2.3-2 risk of ESD damage to electronic
aspirating in CBC or CBC/DIFF mode, 2.2-5 components, 1.2-1
ATL flag sensitivity, C.1-1 CBC
ATTENTION cycle, duration of, 2.1-4
definition, 1.1-3 mode, 2.1-3
audience parameters For In Vitro Diagnostic Use, 2.1-3
requirements of, 1.1-1 parameters For Research Use Only, 2.1-3
reported components, 2.1-1
sampling distribution sequence, 2.2-7
B CBC/DIFF
BA1. See WBC/BASO, threshold cycle, duration of, 2.1-4
BA2. See WBC/BASO, threshold mode, 2.1-3
BA3. See WBC/BASO, threshold parameters For In Vitro Diagnostic Use, 2.1-3
band cells, 2.7-4 parameters For Research Use Only, 2.1-3
bar code reader, 4.23-1 sampling distribution sequence, 2.2-7
checks and adjustments
PN 4237616B INDEX-1
INDEX
absorbance channel, 4.4-7 illustration of, 2.2-2

aperture current, 4.8-1 count and measurement of WBC group, 5.3-5
bath enclosure temperature, 4.16-1 count and waste syringe vacuum, 4.17-1
baths assembly alignment, 4.5-1 count period, measurement characteristics, 2.4-2
count and waste syringe vacuum, 4.17-1 count syringe components, 4.25-1
DIFF lamp voltage, 4.4-3 count vacuum, measurement characteristics, 2.4-2
drain sensor, 4.11-1 counting bath components, 4.35-1
flow cell, 4.4-1 cover, top
Hgb blank, 4.7-1 install, 4.2-5
main card thresholds, 4.14-1 remove, 4.2-4
mix bubble, 4.18-1
motor current, 4.13-1
optical bench assembly, 4.36-1 D
RBC/PLT gain, 4.9-1 DB. See debris
reagent temperature, 4.15-1 DE threshold, 2.7-5
resistive channel, 4.4-5 debris
sample probe, 4.6-1 flag sensitivity, 2.7-3, C.1-1
syringe motor operation, 7.2-1 diagnostic procedures, Not For In Vitro, 2.1-1
transfer sensor, 4.12-1 diameter and length
valve operation, 7.3-1 circuit connections, table of, 6.3-3
WBC/BASO gain, 4.10-1 tubing and connectors, table of, 6.3-3
circuit card layouts, A.2-1 DIFF bath, 2.4-5
Classifications of Etiological Agents on the Basis of aliquot, 2.2-7
Hazards, 1.2-1 dilution characteristics, 2.4-6
cleaning, bath enclosure, 4.28-1 measurement characteristics, 2.4-6
coaxial cable parameter results, 2.4-6
interconnect diagram, 6.5-8 percentages and absolute numbers, 2.1-1
replacement, 4.30-1 second WBC count, 2.4-4
complete blood count, 2.1-1 technical characteristics, 2.4-7
configuring printer settings, 3.2-9 volumes and ratios, 2.3-3
connecting diluent tubing. See initial setup DIFF cable. See DIFF flow cell coaxial cable
connecting reagents DIFF flow cell coaxial cable, interconnect
connecting reagents. See initial setup diagram, 6.5-8
connecting reagents DIFF lamp voltage, 4.4-3
connecting waste tubing. See initial setup differential
connecting waste system DIFF bath, 2.2-3
connector, LCD and keypad, 4.2-7 WBC count, 2.4-5
consumables, table of, 8.1-6 differential lysis with impedance (Coulter
contamination Principle), 2.2-1
risk of differentiation
personal, 1.2-1, 1.2-2, 4.1-1, 4.3-1, 4.3-4, 4. between basophils and other white blood
6-7, 4.27-3, 4.28-1, 4.29-1, 4.34-1, 5.3-1 cells, 2.2-1
continuous sheet feed, printer, 3.3-5 DiffPlot
conventions alarms, 2.7-6
used in this manual, 1.1-3 definition, 2.7-2
Coulter Principle typical, illustration, 2.4-6, 4.4-9
as a method of analysis, 2.4-2 DiffPlot regions, differential
as a technology, 2.2-1 band cells, 2.7-4
general definition, 2.2-1 blast cells, 2.7-4
debris, 2.7-3
2-INDEX PN 4237616B
INDEX
eosinophils, 2.7-3 duration of, 2.1-4

immature granulocytes, 2.7-4
lymphocytes, 2.7-3
monocytes, 2.7-3 F
neutrophils, 2.7-3 +5V voltage, 4.20-4
DiffPlot thresholds 5diff syringe, 4.29-1
absorbance, pertaining to, 2.7-6, C.1-6 5-part leukocyte differential, 2.1-1
definition, 2.7-4 filling of diluent reservoir, 5.3-6
volume, pertaining to, 2.7-5, C.1-5 fitting kit/ PN - XEA311AS, 8.1-8
WBC flow cell balance limit, 4.38-1 fitting. See fitting kit/ PN - XEA311AS
DIL1/HGB bath, 2.4-1 fix cycle
primary and secondary dilutions, 2.4-3 consumption per cycle, 2.1-4
sample reading, 2.5-4 fix reagent circuit, illustration of, 2.8-6
volumes and ratios, 2.3-5 flag sensitivity
diluent and thresholds, C.1-1
consumption per cycle, 2.1-4 default values, C.1-1
reagent circuit, illustration of, 2.8-11 overview, C.1-1
reagent container, 2.1-1 settings, C.2-1
diluent level sensor, interconnect diagram, 6.5-10 flag. See flag sensitivity
diluent reservoir, 4.32-1 flow cell
diluter system as part of the dual focused flow process, 2.2-3
5diff syringe and flow cell, 2.8-12 checks and adjustments, 4.4-1
diluent input, 2.8-11 function, 2.2-3
diluent to baths, 2.8-13 operation, illustration of, 2.4-5
probe and probe rinse, 2.8-13 tolerances and limits, A.1-1
DL threshold, 2.7-5 WBC balance, 4.37-1, 4.38-1
DN threshold, 2.7-5 flow cell coaxial cable
door, main card, 4.2-3 interconnect diagram, 6.5-8
drain sensor, 4.11-1 replacement, 4.30-1
draining bath, 4.34-1 FMN, DiffPlot alarm, 2.7-6
Dual Focused Flow (DFF), 2.2-1, 2.2-3, 2.7-2 FNE, DiffPlot alarm, 2.7-6
DVM (digital voltmeter), 4.16-1 FNL, DiffPlot alarm, 2.7-6
focused flow impedance technology, 2.2-4
For In Vitro Diagnostic Use, 2.1-3
E For Research Use Only, 2.1-3
electrical, 3.1-1 front cover
electromagnetic environment check, 3.1-2 install, 4.2-7
electronic system remove, 4.2-5
LCD and keypad card
LED card
motor interconnect card G
plug/jack labels grounding, electrical, 3.1-2
traverse interconnect card
environment, 3.1-1 H
eosinophils, 2.1-1, 2.2-1, 2.2-4, 2.3-3, 2.4-6, 2.7-3
hardware reset, 4.1-4
EPROMs, 4.39-3
hazards
EPSON® LX™- 300+. See printer
biological, 1.2-1
EPSON® LX™- 300. See printer
electronic, 1.2-1
error messages, 7.1-1
while troubleshooting, 1.2-1
extended cleaning cycles
PN 4237616B INDEX-3
INDEX
Hct, 2.4-2 instrument installation category, 3.1-2

Hct measurement, 2.5-2 instrument labels. See information labels
headings, special intended audience, 1.1-1
definitions, 1.1-3 interconnect diagrams
heater assembly, 4.19-1 bar code reader cable, 6.5-9
hemoglobin, 2.4-3 bath drain and DIFF transfer sensor, 6.5-4
blank adjustment, 4.7-1 DIFF flow cell coaxial cable, 6.5-8
blank reading formula, 2.5-3 diluent level sensor, 6.5-10
calibration factor, 2.5-3 horizontal traverse motor, 6.5-1
dilution characteristics, 2.4-3 horizontal traverse sensor, 6.5-3
measurement characteristics, 2.4-3 IR sensor, 6.5-6
specific flags, 2.5-4 motors and cables, 6.5-1
Hgb B, C.1-1 RBC/WBC coaxial cable, 6.5-7
Hgb Lyse system, 6.4-1
consumption per cycle, 2.1-4 upper fan, 6.5-2
reagent circuit, illustration of, 2.8-5 vertical traverse sensor, 6.5-5
Hgb M, C.1-1 interlock, right side door, 4.1-3
histogram IR sensor, interconnect diagram, 6.5-6
Plt, 2.4-2
RBC, 2.5-1, C.1-7
home position, sample probe, 4.6-2, 4.6-4 J
horizontal traverse jumpers, main card, A.2-5
assembly, 2.3-2
motor, interconnect diagram, 6.5-1 L
sensor, interconnect diagram, 6.5-3
lamp filament projection image, 4.36-4
lamp supply cable, 4.20-2
I lamp, optical bench, 4.36-5
immature cells (IMM), 2.1-1 LCD and keypad card, component locations, A.2-7
immature granulocytes, 2.7-4 LCD and keypad card. See electronic system
IMPORTANT LCD. See electronic system
definition, 1.1-3 LED card, component locations, A.2-8
information labels, location of, 1.2-2 LED card. See electronic system
initial setup left side panel
bypassing manual startup mode, 3.2-9 install, 4.2-3
power on instrument, 3.2-7 remove, 4.2-2
priming instrument, 3.2-8 limits. See tolerances and limits
supplies, 3.2-1 LMD threshold, 2.7-5
unpacking, 3.2-1 LMN threshold, 2.7-5
verification of instrument labels, 3.2-1 LMU threshold, 2.7-5
verification of startup results, 3.2-10 LN
Warning and Caution label locations, 3.2-2 flag sensitivity, C.1-1
inside bath position, sample probe, 4.6-3, 4.6-5 threshold, 2.7-5
inspection of shipment, 3.2-1 lymphocytes, 2.1-1, 2.2-1, 2.2-4, 2.4-6, 2.7-3
inspection report, 3.1-2
Installation Kit/ PN - XEA484AS, 8.1-10 M
installing printer, 3.3-1
MACRO
installing reagent bottles. See initial setup
flag sensitivity, C.1-1
connecting reagents
RBC distribution flags, 2.5-3
instrument at rest, 5.3-3
4-INDEX PN 4237616B
INDEX
macrocytosis, 2.5-3 calculation, 2.5-2

main card measurement characteristics
(all) settings, table of, 4.39-7 DIFF bath, 2.4-6
door, 4.2-3 hemoglobin, 2.4-3
jumper settings, A.2-5 RBC bath, 2.4-2
plug/jack connections, table of, 4.39-3 WBC/BASO bath, 2.4-4
potentiometers, A.2-4 measurement technologies, table of, 2.2-1
replacement, 4.39-1 mechanical and hydraulic modules, A.3-3
supply voltages, 4.20-4 menu item, selecting, 1.1-4, 2.10-1
test points, A.2-2 menu system
threshold adjustments, 4.14-1 keypad, control panel, 2.1-2
main menu options, 2.10-1 LCD, 2.1-2
maintenance kit menu tree, service, 2.10-3
1 year/ PN - XEA486AS, 5.1-1, 8.1-11 menu tree, software, A.4-1
6 months/ PN - XEA485AS, 5.1-1, 8.1-11 menu tree, user, 2.10-2
every 2 years/ PN - XEA581AS, 5.1-1, 8.1-11 MIC. See microcytes
maintenance schedules MICRO
table of, 5.1-1 flag sensitivity, C.1-1
maintenance worklist (left side compartment) RBC distribution flags, 2.5-3
5diff syringe assembly replacement, 5.2-5 microcytes, 2.6-3, 2.6-4
count syringe assembly replacement, 5.2-5 microcytosis, 2.5-3
flow cell coaxial cable replacement, 5.2-7 MN
interim verification check, 5.2-6 alarms, 2.7-6, C.1-7
optical bench lamp replacement, 5.2-6 flag sensitivity, C.1-1
preparation, 5.2-3 threshold, 2.7-5
reagent syringes assembly replacement, 5.2-4 modes of operation, 2.1-3
maintenance worklist (right side compartment) monocytes, 2.1-1, 2.2-1, 2.2-4, 2.4-6, 2.7-3
clean bath enclosure, 5.2-11 motor current, 4.13-1
counting heads, 5.2-11 motor interconnect card
diluent reservoir, 5.2-9 component locations, illustration of, A.2-9
draining baths, 5.2-11 motor interconnect card. See electronic system
interim verification check, 5.2-10 motor voltage
preparation, 5.2-7, 5.2-10 tolerances and limits, A.1-1
sample probe and rinse block assembly, 5.2-8 motors and cables, interconnect diagrams, 6.5-1
sample syringe assembly, 5.2-8
waste syringe assembly, 5.2-9
manual N
abbreviations used in, ABBREVIATIONS-1 NE
Notice of Information Update, function of, 1.1-1 alarms, 2.7-6, C.1-7
numbering format, 1.1-2 flag sensitivity, C.1-1
online vs. printed, 1.1-2 threshold, 2.7-6
organization, 1.1-1 neutrophils, 2.1-1, 2.2-1, 2.2-4, 2.4-6, 2.7-3
reference designators used NL
in, ABBREVIATIONS-1 alarms, 2.7-6, C.1-7
scope, 1.1-1 flag sensitivity, C.1-1
Manual Startup mode, 4.39-1, 4.39-4 threshold, 2.7-6
MCH (mean cell hemoglobin), calculation of, 2.5-4 Note
MCHC (mean cell hemoglobin concentration), definition, 1.1-3
calculation of, 2.5-4 numbering format
MCV, 2.4-2 description, 1.1-2
PN 4237616B INDEX-5
INDEX
O piston, count syringe, 4.25-5

online manual piston, reagent syringe, 4.24-2
part number, 1.1-1 plastic blockers, 3.2-2
open-vial, 2.1-1 platelet
operational cycles, duration of, 2.1-4 histogram, 2.6-1
Operator’s Guide, 2.6-4, 3.2-1, 3.2-9, C.1-2 histogramic area, 2.6-2
optical bench assembly MPV (mean platelet volume)
checks and adjustments, 4.22-1 measurement, 2.6-2
disassembly, 4.22-1 Pct (plateletcrit) calculation, 2.6-2
replacement, 4.22-1 PDW (platelet distribution width)
optical bench lamp, 4.31-1 calculation, 2.6-2
optical measurement, 2.2-4 Plt count, 2.6-1
optical preamplifier card, 2.9-1 platelet clumps, 2.6-5
component locations, illustration of, A.2-6 platelet distribution curve, 2.6-1
optical pulse, 2.2-4 interference with no distinct valley, 2.6-5
o-ring, 5diff syringe, 4.29-3 lower end interference, 2.6-3
o-ring, count syringe, 4.25-5 microcytic interference with distinct valley
o-ring, counting bath, 4.35-3 (below 18fL), 2.6-4
o-ring, counting head aperture, 4.35-5 normal, 2.6-3
o-ring, diluent reservoir, 4.32-2 upper end interference, 2.6-3
o-ring, drain bath, 4.34-1 platelet distribution width (PDW), 2.1-1, 2.4-2
o-ring, reagent syringe, 4.24-2 plateletcrit, 2.1-1
o-ring, rinse block assembly, 4.26-3 Plt threshold, C.1-2
o-ring, sample syringe, 4.33-3 plug/jack labels. See electronic system
pneumatics/hydraulics
circuit connections, table of, 6.3-3
P tubings and connectors, table of, 6.3-1
panel, left side potentiometers
install, 4.2-3 R11 (optical bench assembly), 4.4-3
remove, 4.2-2 R133, R135, 4.9-1
panel, rear access R134, 4.10-1
install, 4.2-4 R136, 4.4-5
remove, 4.2-4 R149 through R156, 4.13-1
parameters R157 through R161, 4.14-1
For In Vitro Diagnostic Use, 2.1-3 R248, 4.7-2
For Research Use Only, 2.1-1, 2.1-3 R286, 4.12-1
part numbers R287, 4.11-1
online manual, 1.1-1 See also main card, potentiometers
Service Resource Kit CD-ROM, 1.1-1 power down, On/Off switch, 4.1-3
parts power on instrument, 3.2-7
nonreturn, table of, 8.1-2 power requirements, 3.1-2
return, table of, 8.1-1 power supply, 4.20-1
parts lists, 8.1-1 power up, 4.1-4
Pct (plateletcrit), 2.4-2 precursors, 2.2-4
PDW (platelet distribution width), 2.1-1, 2.4-2 preinstallation checks
percentage of cells, C.1-1 deficiencies noted during, 3.2-1
peripherals, table of, 8.1-6 electrical, 3.1-1
permanent rinse flow (PRF), 5.3-6 electromagnetic environment check, 3.1-2
pin assignments, B.2-1 environment, 3.1-1
6-INDEX PN 4237616B
INDEX
inspection report, 3.1-2 reaction temperature, summary of dilution

instrument installation category, 3.1-2 characteristics, 2.4-7
power requirements, 3.1-2 reagent lot numbers, entering, 3.2-8
space and accessibility requirements, 3.1-1 reagent syringes, 4.24-1
preliminary adjustments, optical bench reagent temperature, 4.15-1
assembly, 4.36-1 reagents, usage per cycle, 2.1-4
preparation for shipping instrument rear access panel
baths bleaching, 4.3-1 install, 4.2-4
external surfaces cleaning, 4.3-2 remove, 4.2-4
tubing and chambers cleaning, 4.3-2 reference designators
primary dilution, 2.4-1 used in this manual, ABBREVIATIONS-1
prime cycles replacement
duration of, 2.1-4 5diff syringe, 4.29-1
priming instrument, 3.2-8 count syringe components, 4.25-1
printer counting bath components, 4.35-1
configuring, 3.2-9 diluent reservoir, 4.32-1
configuring LX300, 3.3-7 draining bath, 4.34-1
configuring LX300+, 3.3-7 flow cell coaxial cable, 4.30-1
connection to instrument, 3.3-3 heater assembly, 4.19-1
knob installation, 3.3-1 main card, 4.39-1
paper feed options, 3.3-4 optical bench assembly, 4.22-1
ribbon cartridge installation, 3.3-2 optical bench lamp, 4.31-1
unpacking, 3.3-1 power supply, 4.20-1
probe reagent syringes, 4.24-1
diluent reagent circuit, illustration of, 2.8-13 sample probe and rinse block
rinse reagent circuit, illustration of, 2.8-9 components, 4.26-1
publications sample syringe, 4.33-1
on decontamination, 1.2-1 start switch, 4.21-1
waste syringe components, 4.27-1
reproducibility CV limits, 3.2-10, 4.39-8
R reset
ratios. See bath dilutions, volumes and ratios hardware, 4.1-4
RBC system, 4.1-4
bath, 2.2-2, 2.3-5 right side door
distribution flags, 2.5-2 disabling interlock, 4.1-2
histogram, 2.4-2, 2.5-1, C.1-7 opening, 4.2-1
measurement characteristics, 2.4-2 reactivating interlock, 4.1-3
parameter results, 2.4-2 rinse
technical characteristics, 2.4-7 consumption per cycle, 2.1-4
volumes and ratios, 2.3-5, 2.4-2, 2.4-7 reagent supply circuit, illustration of, 2.8-8
RBC/PLT rinse block components, 4.26-2
analysis, 2.4-1 RMN threshold, 2.7-6
dilution cycle DIL1/HGB, 2.2-2
gain, 4.9-1
group count, 5.3-6 S
RBC/WBC coaxial cable, interconnect safety precautions
diagram, 6.5-7 biological, 1.2-1, 1.2-2, 4.1-1, 4.3-1, 4.3-4, 4.6-
RBC1 and RBC2 thresholds, 2.5-3 7, 4.27-3, 4.28-1, 4.29-1, 4.34-1, 5.3-1
RDI (red cell distribution index), C.1-7 electrical, 1.2-1, 4.1-1, 4.1-3
RDW (red cell distribution width), 2.5-2 electronic, 1.2-1
PN 4237616B INDEX-7
INDEX
troubleshooting, 1.2-1 startup cycle, duration of, 2.1-4

warning and information labels, illustration summary of dilution characteristics, 2.4-7
of, 1.2-2 system configuration (default values)
while performing maintenance or default values
service, 1.2-1, 1.2-2, 4.1-1, 4.3-1, 4.3-4, 4.6- See system configuration
7, 4.27-3, 4.28-1, 4.29-1, 4.34-1, 5.3-1 system interconnect diagram, 6.4-1
sample analysis system reset, 4.1-4
overview, 2.2-5 system reset cycle, duration of, 2.1-4
partitioning (CBC and CBC/DIFF modes), system verification procedures
illustration of, 2.2-7 calibration, 5.3-2
sample analysis, creating preparation, 5.3-1
aspirating from first dilution, 2.3-4 reproducibility, 5.3-1
begin aspirating, 2.3-2 startup, 5.3-1
double rinsing probe, 2.3-4 step-by-step cycle check, 5.3-3
making DIFF bath dilution, 2.3-3
making RBC/Plt dilution, 2.3-5
making RBC/PLT first dilution, 2.3-2 T
making WBC/BASO dilution, 2.3-3 +12V voltage, 4.20-4
post aspiration exterior probe rinsing, 2.3-2 -12V voltage, 4.20-4
rinsing exterior of probe, 2.3-5 +24V voltage, 4.20-4
start condition for all baths, 2.3-1 35°C (95°F), regulated temperature of, 2.2-5
start condition for probe and LED, 2.3-1 tangential flow, 2.2-2, 2.2-6
sample preparation (making the dilutions), 5.3-3 test points
sample probe and rinse block components, 4.26-1 TP13, TP14, 4.14-1
sample probe, checks and adjustments, 4.6-1 TP2 through TP4, 4.14-1
sample syringe, 4.33-1 TP40 through TP43, 4.20-4
schistocytes, 2.6-3, 2.6-5 TP48, 4.12-1
SCL. See schistocytes TP5 through TP12, 4.13-1
screws kit/ PN - XEA293AS, 8.1-8 TP52, 4.11-1
screws. See screws kit/ PN - XEA293AS See also main card, test points
secondary dilution, 2.4-1 thresholds
sequential dilution system (SDS), 2.2-6 and flag sensitivity, C.1-1
service and repair, guidelines, 4.1-1 definition, 2.2-3
service memo, 1.1-1 description, 2.2-4
service menu tree, 2.10-3 main card, 4.14-1
Service Resource Kit CD-ROM, 1.1-1 Plt, C.1-2
shutdown cycle, duration of, 2.1-4 settings, C.3-1
signal processing, 2.2-4 voltage limits, A.1-1
single sheet feed, 3.3-4 WBC flow cell balance limit, 4.38-1
SL WBC/BASO, C.1-2
flag sensitivity, C.1-1 See also DiffPlot thresholds
threshold, 2.7-5 tolerances and limits
SL1 flag sensitivity, C.1-1 calibration factors, A.1-2
software flow cell, A.1-1
menu tree, 2.10-1, A.4-1 motor voltage, A.1-1
overview, 2.10-1 power supply voltages, A.1-2
space and accessibility requirements, 3.1-1 thresholds voltage limits, A.1-1
spectrophotometric technique, 2.4-3 whole blood reproducibility CV limits, A.1-2
start switch, 4.21-1 tools and supplies, 4.1-2
tools, table of, 8.1-7
8-INDEX PN 4237616B
INDEX
top cover W
install, 4.2-5 WARNING
remove, 4.2-4 biological risk of personal
transfer sensor, 4.12-1 injury, 1.2-1, 1.2-2, 4.1-1, 4.3-1, 4.3-4, 4.6-7,
traverse interconnect card 4.27-3, 4.28-1, 4.29-1, 4.34-1, 5.3-1
component locations, illustration of, A.2-10 definition, 1.1-3
traverse interconnect card. See electronic system electronic risk of personal
tubing and chambers cleaning injury, 1.2-1, 4.1-1, 4.1-3
burn-in cycles, 4.3-3 warning labels
drain and rinse, 4.3-3 location of, illustration, 1.2-2
preparation, 4.3-2 washer, count syringe, 4.25-5
washer, diluent reservoir, 4.32-2
U washer, reagent syringe, 4.24-2
UM waste alarm installation. See initial setup
flag sensitivity, C.1-1 connecting waste system
threshold, 2.7-5 Waste Alarm Kit/ PN - 6912680, 8.1-11
UN waste container, 2.1-1
flag sensitivity, C.1-1 waste syringe components, 4.27-1
threshold, 2.7-5 waste system
unpacking, 3.2-1 description, 2.8-15
upper fan, interconnect diagram, 6.5-2 illustration of, 2.8-15
user menu tree, 2.10-2 WBC
user mode, 3.2-9, 4.1-2, 5.2-1 flag sensitivity, C.1-1
WBC count
balance of, 4.37-1
V calculation of, 2.7-1
valves WBC Lyse
functions, 2.8-3 consumption per cycle, 2.1-4
location of, 2.8-1 reagent circuit, illustration of, 2.8-7
operation, 7.3-1 WBC/BASO
verification of sequential valve activation, 7.3-2 bath, 2.2-1, 2.2-2, 2.2-5, 4.38-1
verification of dilution characteristics, 2.4-4
baths assembly alignment, 4.5-1 gain, 4.10-1
flow cell adjustments, 4.4-9 histogram, 2.4-2, 2.4-5
flow cell, bubble free, 4.36-2 measurement characteristics, 2.4-4
instrument labels, 3.2-1 parameter results, 2.4-4
sequential valve activation, 7.3-2 reference WBC count, 2.4-4
startup results, 3.2-10 rinse reagent circuit, illustration of, 2.8-10
supply voltages, 4.20-4 technical characteristics, 2.4-7
syringe motor operation, 7.2-3 threshold, C.1-2
system, 5.3-1 volumes and ratios, 2.3-3, 2.4-7
vertical traverse white blood cell, differential, 2.1-1
assembly, 2.3-2 whole blood reproducibility CV limits
sensor, interconnect diagram, 6.5-5 tolerances and limits, A.1-2
voltages, power supply, 4.20-4, A.1-2 worklist instructions
volume aperture, 2.2-1 basic, 5.2-2
maintenance category identifier, 5.2-2
overview, 5.2-2
worklist. See maintenance worklist
PN 4237616B INDEX-9
INDEX
wrap up, system verification procedures, 5.3-6
X
X-axis, optical bench assembly, 4.36-3
Y
Y-axis, optical bench assembly, 4.36-2
10-INDEX PN 4237616B
TRADEMARKS
The BECKMAN COULTER logo and AC•T are trademarks of Beckman Coulter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.
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BECKMAN COULTER™ AC•T™ 5diff

PN 4237616B (September 2000)

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

WARNING - Might cause injury.

Copyright © Beckman Coulter, Inc. 1999-2000

Initial Issue, 3/2000

Changes were made on the following pages:

REVISION STATUS, iii

1.1 MANUAL DESCRIPTION, 1.1-1

1.2 SAFETY PRECAUTIONS, 1.2-1

2 INSTRUMENT DESCRIPTION, 2.1-1

2.1 INTRODUCTION TO THE AC•T 5diff HEMATOLOGY ANALYZER, 2.1-1

2.2 OPERATION PRINCIPLES, 2.2-1

Signal Processing, 2.2-4

2.3 CYCLE DESCRIPTION, 2.3-1

2.4 SAMPLE ANALYSIS, 2.4-1

2.5 RBC PARAMETER DEVELOPMENT, 2.5-1

2.6 PLATELET PARAMETER DEVELOPMENT, 2.6-1

Detecting Abnormal Platelet Distributions, 2.6-3

2.7 WBC PARAMETER DEVELOPMENT, 2.7-1

2.8 PNEUMATIC/HYDRAULIC SYSTEM, 2.8-1

2.9 ELECTRONIC SYSTEM, 2.9-1

2.10 SOFTWARE STRUCTURE, 2.10-1

3 INSTALLATION PROCEDURES, 3.1-1

3.1 PREINSTALLATION CHECKS, 3.1-1

3.2 INITIAL SETUP, 3.2-1

3.3 PRINTER INSTALLATION, 3.3-1

4 SERVICE AND REPAIR PROCEDURES, 4.1-1

4.1 GUIDELINES FOR SERVICING THE AC•T 5diff HEMATOLOGY

4.2 OPENING OR REMOVING INSTRUMENT DOORS, PANELS, AND

4.3 PREPARATION TO SHIP THE INSTRUMENT, 4.3-1

4.4 FLOW CELL CHECKS AND ADJUSTMENTS, 4.4-1

4.5 BATHS ASSEMBLY ALIGNMENT CHECK AND ADJUSTMENT, 4.5-1

4.6 SAMPLE PROBE CHECKS AND ADJUSTMENTS, 4.6-1

4.7 HGB BLANK ADJUSTMENT, 4.7-1

4.8 APERTURE CURRENT CHECK, 4.8-1

4.9 RBC/PLT GAIN ADJUSTMENT, 4.9-1

4.10 WBC/BASO GAIN ADJUSTMENT, 4.10-1

4.11 DRAIN SENSOR ADJUSTMENT, 4.11-1

4.12 TRANSFER SENSOR ADJUSTMENT, 4.12-1

4.13 MOTOR CURRENT ADJUSTMENT, 4.13-1

4.14 THRESHOLD ADJUSTMENTS, 4.14-1

4.15 REAGENT TEMPERATURE CHECK AND ADJUSTMENT, 4.15-1

4.16 BATH ENCLOSURE TEMPERATURE CHECK AND ADJUSTMENT, 4.16-1

Bath Enclosure Temperature Check, 4.16-1

4.17 VACUUM CHECKS AND ADJUSTMENTS, 4.17-1

4.18 MIX BUBBLE ADJUSTMENT, 4.18-1

4.19 HEATER ASSEMBLY REPLACEMENT, 4.19-1

4.20 POWER SUPPLY REPLACEMENT, 4.20-1

4.21 START SWITCH REPLACEMENT, 4.21-1

4.22 OPTICAL BENCH DISASSEMBLY AND REPLACEMENT, 4.22-1

4.23 BAR-CODE READER TESTING AND CONFIGURATION, 4.23-1

4.24 REAGENT SYRINGES ASSEMBLY REPLACEMENTS, 4.24-1

4.25 COUNT SYRINGE COMPONENT REPLACEMENTS, 4.25-1

4.26 SAMPLE PROBE AND RINSE BLOCK ASSEMBLY COMPONENT

4.27 WASTE SYRINGE COMPONENT REPLACEMENTS, 4.27-1

4.28 CLEANING THE BATH ENCLOSURE, 4.28-1

4.29 5diff SYRINGE ASSEMBLY REPLACEMENTS, 4.29-1