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    Lab #4 Analysis of Food Dyes in Beverages: Sophia Whitesel Mrs. Haberman AP Chemistry Pd. 5 October 4, 2016

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    This lab report summarizes an experiment to determine the concentration of food dye in Gatorade using a spectrophotometer. Stock solutions of the dye FD&C Blue 1 were prepared at different concentrations and their absorbance measured. This generated a calibration curve relating concentration to absorbance. The absorbance of a Gatorade sample was then measured and used to determine its dye concentration from the calibration curve. The concentration of Blue 1 in Gatorade was found to be 0.298 micro-molar.

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    Lab #4 Analysis of Food Dyes in Beverages: Sophia Whitesel Mrs. Haberman AP Chemistry Pd. 5 October 4, 2016

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    This lab report summarizes an experiment to determine the concentration of food dye in Gatorade using a spectrophotometer. Stock solutions of the dye FD&C Blue 1 were prepared at different concentrations and their absorbance measured. This generated a calibration curve relating concentration to absorbance. The absorbance of a Gatorade sample was then measured and used to determine its dye concentration from the calibration curve. The concentration of Blue 1 in Gatorade was found to be 0.298 micro-molar.

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    This lab report summarizes an experiment to determine the concentration of food dye in Gatorade using a spectrophotometer. Stock solutions of the dye FD&C Blue 1 were prepared at different concentrations and their absorbance measured. This generated a calibration curve relating concentration to absorbance. The absorbance of a Gatorade sample was then measured and used to determine its dye concentration from the calibration curve. The concentration of Blue 1 in Gatorade was found to be 0.298 micro-molar.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    125 views3 pages

    Lab #4 Analysis of Food Dyes in Beverages: Sophia Whitesel Mrs. Haberman AP Chemistry Pd. 5 October 4, 2016

    Uploaded by

    api-313696140
    This lab report summarizes an experiment to determine the concentration of food dye in Gatorade using a spectrophotometer. Stock solutions of the dye FD&C Blue 1 were prepared at different concentrations and their absorbance measured. This generated a calibration curve relating concentration to absorbance. The absorbance of a Gatorade sample was then measured and used to determine its dye concentration from the calibration curve. The concentration of Blue 1 in Gatorade was found to be 0.298 micro-molar.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Lab #4

    Analysis of Food Dyes in Beverages

    Sophia Whitesel
    Mrs. Haberman
    AP Chemistry pd. 5
    October 4, 2016
    Objective
    The concentration of the dye present in Gatorade is to be determined by using a
    spectrophotometer, wavelength, transmittance, and absorption.

    Introduction

    There are many different types of wavelengths which are visible between 350 and 800 units.
    Some examples of wavelengths are: cosmic rays, gamma rays, x-rays, infrared rays, radio waves,
    and electric waves. The visible color wavelengths range from violet (blue, green, yellow, orange)
    to red. “The white light is a mixture of the colors of the visible spectrum. Black is a total absence
    of light” (http://science-edu.larc.nasa.gov). Violet has the smallest waves and red has the largest
    waves. “A spectrophotometer is an instrument that shines a single wavelength of light of a
    known intensity into a solution and then measures the intensity of the light exiting the solution. If
    a solution contains any compound that absorbs light, the intensity of light exiting the solution
    will be less than what entered the solution. On the other hand, if none of the compounds in the
    solution absorb at that wavelength of light, then the intensity of the light exiting the solution will
    be the same as that entering” (faculty.ccbcmd.edu).

    Materials
    FD&C Blue 1 stock solution (50-mL), Blue consumer sports drink (10mL), distilled water, nine
    test tubes, two to three 50-mL beakers, 10-mL pipet (serological), pipet bulb or pipet filler,
    spectrophotometer, and test tube rack.

    Procedure
    First, students took nine test tubes. The test tubes were each filled with the directed amount of
    distilled water and stock solution (solution A: 0mL of water and 10mL of stock solution, solution
    B: 2mL of water and 8mL of stock solution, solution C: 4mL of water and 6mL of stock solution,
    solution D: 6mL of water and 4mL of stock solution, solution E: 7mL of water and 3mL of stock
    solution, solution F: 8mL of water and 2mL of stock solution, solution G: 9mL of water and 1mL
    of stock solution, solution H: 10mL of water and 0mL of stock solution, Gatorade solution: 0mL
    of water and 10mL of blue Gatorade), and then placed in the test tube rack. Next, the students set
    up the spectrophotometer. The wavelength was set to 630. One by one, each of the test tubes was
    placed in the spectrophotometer. The absorbance and concentration of the solution was then
    recorded.

    The picture above shows the solutions that were used to obtain the data for this experiment.
    Results

    Calculations:
    M1V1=M2V2
    Concentration of Solution A= 7uM
    Concentration of Solution B= (7)(8)/10= 5.6uM
    Concentration of Solution C= (7)(6)/10= 4.2uM
    Concentration of Solution D= (7)(4)/10= 2.8uM
    Concentration of Solution E= (7)(3)/10= 2.1uM
    Concentration of Solution F= (7)(2)/10= 1.4uM
    Concentration of Solution G= (7)(1)/10= 0.7uM
    Concentration of Solution H= (7)(0)/10= 0uM

    Conclusion
    Based on the graphs obtained from the lab, the optimum liner-relationship or calibration curve
    for the graph representing the stock solution and the blue Gatorade is y=0.0308x. The dyes
    present in the beverage was Blue 1 food dye. This proves that the absorbance measurements
    were accurately obtained by the group because that value of .298 is between .2 and 1.0. This can
    be proven by using a spectrophotometer. The absorbance recorded is between the range of 0 to
    0.3. The point at zero represents the solution without the stock solution and and the Gatorade had
    an absorbance of 0.3. The concentration was found to be .298 micro-molar and .236mg/L.

    Literature Cited

    "Experiment 3: Concentration in Gatorade." CCBC Faculty Web. N.p., n.d. Web. 03 Oct. 2016.

    "What Wavelength Goes With a Color." NASA. NASA, n.d. Web. 03 Oct. 2016.

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