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    Pharma Research & Analytical Laboratories: 5, Old Trunk Road, Pallavaram, Chennai-600 043 Disposal of Culture and Media

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    Manivannan Kathirvel
    The document outlines procedures for the disposal of used culture media and plates at Pharma Research & Analytical Laboratories. It describes sterilizing used media in an autoclave, removing and diluting the contents, and draining the liquid into an effluent treatment plant. Empty plates and tubes are sterilized as well and may be reused after drying or discarded. The microbiologist is responsible for media disposal according to the daily procedure to eliminate contamination risks.

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    Pharma Research & Analytical Laboratories: 5, Old Trunk Road, Pallavaram, Chennai-600 043 Disposal of Culture and Media

    Uploaded by

    Manivannan Kathirvel
    93 views2 pages
    The document outlines procedures for the disposal of used culture media and plates at Pharma Research & Analytical Laboratories. It describes sterilizing used media in an autoclave, removing and diluting the contents, and draining the liquid into an effluent treatment plant. Empty plates and tubes are sterilized as well and may be reused after drying or discarded. The microbiologist is responsible for media disposal according to the daily procedure to eliminate contamination risks.

    Original Description:

    DISPOSAL OF MICROBIOLOGICAL MEDIA

    Original Title

    Disposal Media

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    The document outlines procedures for the disposal of used culture media and plates at Pharma Research & Analytical Laboratories. It describes sterilizing used media in an autoclave, removing and diluting the contents, and draining the liquid into an effluent treatment plant. Empty plates and tubes are sterilized as well and may be reused after drying or discarded. The microbiologist is responsible for media disposal according to the daily procedure to eliminate contamination risks.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    93 views2 pages

    Pharma Research & Analytical Laboratories: 5, Old Trunk Road, Pallavaram, Chennai-600 043 Disposal of Culture and Media

    Uploaded by

    Manivannan Kathirvel
    The document outlines procedures for the disposal of used culture media and plates at Pharma Research & Analytical Laboratories. It describes sterilizing used media in an autoclave, removing and diluting the contents, and draining the liquid into an effluent treatment plant. Empty plates and tubes are sterilized as well and may be reused after drying or discarded. The microbiologist is responsible for media disposal according to the daily procedure to eliminate contamination risks.

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    You are on page 1of 2

    PHARMA RESEARCH & ANALYTICAL LABORATORIES

    5, OLD TRUNK ROAD, PALLAVARAM, CHENNAI-600 043


    QUALITY CONTROL DISPOSAL OF CULTURE AND MEDIA
    QC/MCB/017/01 Revision :Original Supercedes :Nil

    Effective Date : MAY-13 Review Date : APR-15 Page No. : 1 of 2

    1.PURPOSE : To lay down a procedure for proper disposal of used media


    plates and culture tubes.
    2.SCOPE : This SOP is applicable for Media disposal.

    3.RESPONSIBILITY : Microbiologist

    4.ACCOUNTABILITY :QC Manager


    5.PROCEDURE :
    a. Wear rubber hand gloves and nose mask.

    b. Sterilise the used media plates in autoclave at 15 psi, 121 °C for 30


    minutes.

    c. Remove media content of Petri dish with the help of spatula and
    collect in a container.

    d. Add 5% dettol solution to the collected waste media and dilute the
    autoclaved contents with tap water and drain into the ETP plant.

    e. Keep the empty glass Petri dishes for 1 hr in 5% dettol solution or


    savlon solution and then wash the Petri dish with water and dry them for
    reuse. Disposable plates should be discarded after keeping them for 1 hr
    in 5% dettol or savlon solution

    CULTURE TUBES
    a.Autoclave the microbial media tubes at 15 psi, at 121 °C for
    30 minutes.
    b.After autoclaving add 5% dettol solution to the media and dilute the
    autoclaved contents with tap water and drain into the ETPplant.

    PREPARED BY CHECKED BY APPROVED BY

    N.U.VIJAYALAKSHMI E.LEELAVATHY K.MANIVANNAN


    Microbiologist Manager QC DGM QC
    Dt: Dt: Dt:
    PHARMA RESEARCH & ANALYTICAL LABORATORIES
    5, OLD TRUNK ROAD, PALLAVARAM, CHENNAI-600 043
    QUALITY CONTROL DISPOSAL OF CULTURE AND MEDIA
    QC/MCB/017/01 Revision :Original Supercedes :Nil

    Effective Date : MAY-13 Review Date : APR-15 Page No. : 2 of 2

    c. The empty tubes are dry them for reuse.


    d.After completion of Autoclave cycle for discarded media/cultured
    tube remove the culture tube/media from the autoclave and start a
    blank autoclave cycle without material to eliminate the possibility of
    any contamination.
    e. Media preparation and media decontamination (used media discard)
    activities shall not be done simultaneously.
    6.FERQUENCY : Daily
    7.END OF THE DOCUMENT

    PREPARED BY CHECKED BY APPROVED BY

    N.U.VIJAYALAKSHMI E.LEELAVATHY K.MANIVANNAN


    Microbiologist Manager QC DGM QC
    Dt: Dt: Dt:

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