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    Materials and Methods Collection of Medicinal Plants

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    sumi
    The document describes the materials and methods used to study the antibacterial properties of Moringa oleifera plant extracts. Fresh Moringa oleifera plants were collected from various locations in Tamil Nadu, India. Leaf extracts were prepared using different solvents like ethanol, n-butyl alcohol, and acetone. The extracts were tested against five human pathogens - Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella typhi - to evaluate their antibacterial activity using the disc diffusion method.

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    Materials and Methods Collection of Medicinal Plants

    Uploaded by

    sumi
    19 views3 pages
    The document describes the materials and methods used to study the antibacterial properties of Moringa oleifera plant extracts. Fresh Moringa oleifera plants were collected from various locations in Tamil Nadu, India. Leaf extracts were prepared using different solvents like ethanol, n-butyl alcohol, and acetone. The extracts were tested against five human pathogens - Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella typhi - to evaluate their antibacterial activity using the disc diffusion method.

    Original Description:

    Moringa Materials and methods

    Original Title

    Yamaha Industry

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    The document describes the materials and methods used to study the antibacterial properties of Moringa oleifera plant extracts. Fresh Moringa oleifera plants were collected from various locations in Tamil Nadu, India. Leaf extracts were prepared using different solvents like ethanol, n-butyl alcohol, and acetone. The extracts were tested against five human pathogens - Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella typhi - to evaluate their antibacterial activity using the disc diffusion method.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    19 views3 pages

    Materials and Methods Collection of Medicinal Plants

    Uploaded by

    sumi
    The document describes the materials and methods used to study the antibacterial properties of Moringa oleifera plant extracts. Fresh Moringa oleifera plants were collected from various locations in Tamil Nadu, India. Leaf extracts were prepared using different solvents like ethanol, n-butyl alcohol, and acetone. The extracts were tested against five human pathogens - Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella typhi - to evaluate their antibacterial activity using the disc diffusion method.

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    © All Rights Reserved
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    of 3

    MATERIALS AND METHODS

    COLLECTION OF MEDICINAL PLANTS :-

    Fresh and healthy plants were collected for over three months time (Oct-
    Dec 2017) from various locations of the kanyakumari District (Attoor) of these
    plants two medicinal plants were selected for the study.

    Andrographics paniculata, Nees.

    Division - Magnoliophyta

    Class - Magnoliopsida

    Sub Class - Dilleniidae

    Order - Capparales

    Family - Moringaceae

    Genus - Moringa

    Species - oleifera

    Moringa oleifera is the most widely cultivated species of a monogeneric


    family, the Moringaceae, which is native to the Sub – Himalayan tracts of India,
    Pakistan, Bangladesh and Afghanistan. India is the largest producer of Moringa
    with an animal production of 1.1 to 1.3 million tones of tender fruits from an area
    of 380 Km2.
    Moringa oleifera is a short, slender, deoduous, perennial tree to about 10m
    tall rather slender with drooping branches corhy bark, leaves fieathery, pale green,
    compound, tripinnate 30, 60cm long with many small leaflets. Flowers fragrant,
    white or creamy, stamens yellow pods pendulous, main root thick.

    PREPARATION OF EXTRACT

    Freshly collected plant leaves were shade dried for ten days and grind. The
    grind powder was soaked in 10ml of different solvents such as ethanol, n-butyl
    alcohol, iso - propyl alcohol, benzene and acetone. All the solvent extracts were
    kept at room temperature for 10 days with periodic shacking to extract the
    compounds from the powdered material. The extracts were obtained by passing
    through the muslin cloth, the respective solvents were evaporated with the help of
    heating mantle. Then it was stored in air tight bottles for further study.

    PREVENTION OF NATURAL DISC

    Sterile discs were obtained and stored at 4°C. Discs were handled using a
    pair of presterilized forceps. The extract was loaded on to the disc carefully using
    capillary tube, without spreading out. Thus the disc completely saturated with the
    extract was used for testing antibacterial activity.

    SYNTHETIC DISC

    The synthetic discs used were chloramphenicol, Kanamycin and neomycin.


    HUMAN PATHOGENIC USED

    A total of five human pathogens were used in the study staphylococcus


    aurous, Escherichia coli, proteus vulgaris, pseudomonas aeruginosa and salmonella
    typhi. All human pathogens were purchased from the (Scudder laboratory,
    Nagercoil)

    CULTURE OF TEST HUMAN PATHOGENS (DILUTION)

    An inoculums of each pathogen was suspended in 3m1 of nutrient broth. The


    bacteria species were cultured in the nutrient broth. All culture were incubated at
    37°C for l8hr and then diluted to 1/10 the concentration to yield a culture density
    of approximately 108 CFU/ML.

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