USE OF PINEAPPLE PEELS AS ORGANIC

STAIN REMOVAL AND WHITENING AGENT

ON FABRICS

Conducted by:

Lead Researcher
ANA MARIE O. ALDESIMO

WENNY B. CABANTOG
MARIA REGINA C. ANSAY
 USE OF PINEAPPLE PEELS AS ORGANIC STAIN REMOVAL AND
WHITENING AGENT ON FABRICS

ABSTRACT

This study involves the determination of the effect of pineapple (Ananas comosus) peel
extract, (with and without salt), on fabrics stained with grease, ink, hair dye, deodorant,
mud,and rust. An 8” X 9” cotton white cloth was used. The fabricwassmeared with the
0.01 g stain in the center, (except for the deodorant where 4 strokes were applied). The
different stained fabrics were setaside for 10 hours, after which were treated with 5
grams each of Clorox, and pineapple extract (with and without salt) for 10 to 20 minutes.
This study was limited to the conduct of visual observation. The ranking test was
considered to determine the degree of preference of stain removed. Results showed
that removal of stains depends on the possible reaction of specified stain
againstthechemical composition of anti-stain solutions used. The effect of the above
three solutions gave significant effect as stain removal. Commercially available
Cloroxwasused as the control. This was able to reducemost of the above stains.
However, the degree of reduction depends on the stain. Pineapple waste extract (acid)
and salt (sodium chloride) were effective against rust, mud,and deodorant. The effect of
the fabric whitening was not noticeable from the three stain removal solutions due to the
spread of smears contributed by different stains in the fabrics.

INTRODUCTION

Stains are part of everyday life. There are more than 100 tough stains and effectivity of

stain removals depends on the kind of stain and the chemical reaction once it gets in

contact with the stain removal.These are some of the solvents that can be used for

stains, such as oxidizing solvents (hydrogen peroxide), reducing solvents (sodium

hydrosulfite, sodium hypochlorite,and sodium chloride), lacquer solvent, inert solvents,

detergents, acids, etc. There are different commercially available stain remover but

consumers are not aware of its effectiveness and/or the kind of stains that they are

capable of removing. The common stain remover and bleaching agent is Clorox. The

active ingredient in Clorox, a popular commercialized stain remover, is sodium

hypochlorite which comes from seawater and breaks down into salt and water.

Oxalic acid and acetic acid are cited as possible stain removal when properly used

(Consumer Guide, nd). Oxalic acid's main applications include cleaning or bleaching,
especially for the removal of rust (iron complexing agent). It acts as rust removal agents

due to its forming a stable, water-soluble salt with ferric. Fruits that contain citric acid may

act as stain removal. These are effective on fruit stains as “like dissolves like”. However,

its acid is considered a weaker acid than the oxalic acid. Concentration, pH, time and

temperature are the various conditions that affect the action of bleach on fabrics.

Halvorson, C.(nd), cited that vinegar with salt is effective against rust. Others cited that

grass and ink are removed by vinegar. Halvorson (nd) also mentioned that salt is

recommended to remove grease, ink,anddeodorant. Investigative study (Mendoza, K,

2016) show that pineapple waste with baking soda was effective as stain remover but

needs scrubbing in order for the stain to disappear. However, it was not made mention

of the stains tested.

The use of pineapple waste as stain remover may be very applicable in the Philippines

area, where pineapple is extensively cultivated. Some of these areas are in the

Northern Mindanao, SOCCKSARGEN (Southern Cotabato, Cotabato Province, Sultan

Kudarat, Sarangani, General Santos City), Bukidnon, Bicol and CALABARZON (Cavite,

Laguna, Batangas, Rizal, Quezon). Moreover, it is noted that the Philippines is the

second biggest pineapple-exporting country in the world next to Thailand. In 2009,

57,687 ha were planted to the crop with a total production of 2,198,497. This means that

more wastes are generated.

The use of pineapple waste is noteworthy,thus, this study focuses on the effect of acids

from pineapple wastes and salt on different common stains such as grease, printer ink,

deodorant, hair dye, mud,and rust. It also focuses on the degree ofwhitening once stain

removal is applied. Pineapple contains citric acid, malic acid, ascorbic acid and

pantothenic acid.
Filipinos who can't afford commercialized stain remover could use waste materials such

as pineapple waste. Furthermore, stain remover is oftentimes not available in remote

places. Hence, this study considered acid asthe main ingredient from pineapple wastes

together with salt as a stain removal. Clorox serves as a control. However, this paper

is limited only to visual observations supported by literature reviews. It is hypothesized

(Ho) that there is no significant difference between and among the three stain removers

when treated in fabrics with grease, ink, deodorant, hair dye, mud or rust.

METHODOLOGY

1. Material Preparation and Pineapple Waste Profiling

Commercially available Clorox with 52, 000 ppm sodium hypochlorite was used

as one of the stain removals and/or bleaching agent which served as the control.

Other major agents used were pineapplepeel extract(table ripe) with and without

salt.

Pineapple waste profile such as weights of pineapple peels, core, crown,

pineapple peel extract, core extract was gathered.Ten pineapples were used in

this data gathering. Pure extract and extract with salt were considered in this

experiment. The ratio of pineapple peel extract with salt was 2 parts extract:1-part

salt.

Procedures for preparing the pineapple extract. Two pineapples with almost

the same size and weight were washed and peeled. The pineapple wastes were

weighed and chopped finely. After chopping, the wastes were put inside a clean

white cloth and squeezed to extract the juice.

The acidity of pineapple extract was analyzedusing a pH Meter. Citric acid and

oxalic acid
 were analyzed using titratable acidity test (AOAC Official Method, 942.15, 19th

ed., 2012).

The conduct of titratable analysis was sourced out from a recognized laboratory,

the

Department of Science and Technology (DOST).

2. Preliminary Study

` The following types of stains were used in this study: grease, printer ink, hair

dye, deodorant, mud,and rust. Each stain weighing approximately 5 grams were

spread in a 4” X 4” 100% cotton cloth (white T-shirt). These were treated with

chlorox, pineapple waste extract (with and without salt).

3. Experimental Design and Procedure

3.1. Five yards of cotton cloth was purchased. These were measured and cut

into 8” X 9” and used as fabric to be stained. Each stainwas spread in each

fabric as follows: grease (.01g), ink (.01g), hair dye (.01g), deodorant (4

strokes), mud (.01g) and rust (.01grams).

3.2. Stains were allowed to stay in the cloth for 10 hours. The period of

exposure to stain was based on the actual practice that when the fabric is

blemished it is not usually treated at once.

3.3 After 10 hours, each cloth with stain was treated with approximately 5 grams

each of Clorox,purepineapple extract with salt (2:1) and pure pineapple extract

without salt (both from pineapple peels). The stained cloths were exposed to

different stain remover for 10-20 minutes after which was rinsed under running

water.

Table 1 shows the experimental design. Two trials were conducted.

 Table 1. Experimental design on determination of an effective stain
remover
and bleaching agent

Stains Treatments
Chlorox Pineapple waste Pineapple waste
extract with salt extract without
salt
grease (.01g) XX XX XX
ink (.01g) XX XX XX
hair dye (.01g) XX XX XX
deodorant (4 strokes) XX XX XX
mud (.01g) XX XX XX
rust (.01grams) XX XX XX
Legend: XX – two trials conducted

3.4. Evaluation and Statistical Analysis

The removal of stain in the fabricand the effect as a whitening agent was

evaluated by 20 panels with 20/20 vision. Two trials were made using its visual

observation.

The ranking was used to evaluate the preferred stain most removed with the least

stain removed.Kramers rank sum test was used to determine if there were

differences between and among samples on the stain removed. AnnexA shows

the questionnaire for ranking the most stained removed.

The effect of the different whitening agents was observedon the same stained

fabrics bythe same twenty panelists. The treated fabric was subjected to visual

observation by ranking the panel’s preference: 1 as the most bleached and 3 as

the least bleached. Kramers ranksum test was used to determine if there were

differences between and among thebleached fabric by preference. Annex B

shows the questionnaire for ranking the most preferred bleached fabric.
RESULTS AND DISCUSSION

1. Pineapple Waste Extract Profile

The pineapple waste extract had an approximate pH of 4.9. The said pH falls

within the normal pH range of pineapple juice (3.5 to 5.2 pH). This exhibits less

acidic juice. Maturity played a major role in its high pH content. This means that it

may contribute to a weaker acid. The pineapple sample used was table ripe. It

contains 0.39% citric acid and 0.254% oxalic acid. When the extract was mixed

with salt, it contains 0.366% and 0.235% citric acid and oxalic acid, respectively.

Forty-eight percent (48%) of the raw table ripe pineapplewas the pineapple waste.

The materials used in this experiment was limited only in its peels which were

27% of the whole pineapple (as purchased pineapple, AP). The core was not

included since it could still be used as juice. The extract to be used stain remover

and/or bleaching was 7% of the As Purchased (AP). Table 2shows the profile of

table ripe pineapple.

 Table 2. Table ripe pineapple profile

No Weight of Weight of Weight of Weight of Weight of

Whole Pineapple pineapple Edible
Pineapple, (in grams) extract from extract from parts
AP Peels Core Crown Total peels(grams) core(grams)
(grams)

1 1,600g 390g 110g 260g 400g 90g 40g 790g

2 1,600g 430g 130g 350g 910g 80g 50g 730g

3 1,700g 440g 110g 410g 960g 160g 50g 760g

4 1,650g 530g 130g 340g 1000g 90g 60g 730g

5 1,500g 430g 140g 230g 800g 140g 100g 750g

6 1,650g 420g 120g 330g 870g 130g 60g 740g

7 1,750g 450g 130g 120g 700g 115g 60g 830g

8 1,750g 530g 130g 70g 730g 170g 70g 600g

9 1,750g 490g 140g 100g 730g 120g 100g 800g

10 1,750g 430g 140g 340g 910g 150g 70g 780g

MEAN 1,670g 454 128 255 801g 126g 660g 751g

(27.2%) (7.7%) (15%) (48%) (7.4%) (39.5%) (45%)

2. Preliminary Study

The preliminary study conducted showed that the initial design of the experiment

of using 5 g stain to be spread in the fabric was too thick. Such concentration did

not exhibitareduction of stain. Thus, only 0.01 g stain was spread in the fabric in

the succeeding experiments.

3. Evaluation and Statistical Analysis

Table 3shows the results ofthe ranking based onpreference on stainsfrommost

removed to least removed (lower scores represent most removed). This

Tableshowsthatfabrics stained with grease, ink and hair dye and treated with
Clorox were significantly preferred to have reduced the above stains versus

pineapple extract with and without salt. Annex C shows that Clorox reduced the

stains. The pH of Clorox was around 12 thus the stain was loosened by the

solvents/mixtures. The stain consisting of molecule chains may have been broken

down by the stain removal into smaller pieces which caused the spread in the

fabric but with time and exposure to constant washing and stain removal, the stain

may disappear.

Deodorant stain could be decreased by pineapple waste with salt and result of

statistical analysis (Kramers Rank Sum test) showed that there wasa

significantpreference in terms of stain reduction when compared with the other two

(2) stain removers. This was followed by pineapple waste without salt. This may

be due to the loosening of stain made by the different stain remover but eventually

stains maybe be reduced in the succeeding washing. The mud stains could be

reduced with the application of pineapple waste without salt. The said stain

removal was the most reduced stain on the fabric.Lastly, the majority of the

rustwas removed by pineapple waste without salt. Most probably the active and

effective ingredient was the acid. Contrary to the photo in Annex C, Table 3

exhibited that results of statistical treatment showed pineapple extract with salt

was the least removed

Table 3. Ranking on most preferred stain removal

Stains Treatments Statistical Result

Clorox Pineapple Pineapple Kramers rank sum
waste waste extract test
extract with without salt 5% 32 – 48
salt 1% 30 - 50
grease (.01g) 20* 56* 44 significant
ink (.01g) 20* 45 55* significant
hair dye (.01g) 26* 46 48 significant
deodorant (4 54* 24* 42 significant
strokes)
mud (.01g) 53* 39 28* significant
rust (.01grams) 37 56* 27* significant
Legend: * Significant at 1% level (the least value with asterisk show the most
preferred
reduction of stain, the highest value with asterisk showed the least preferred in
terms of stain reduction)

The same fabrics stained by the different materials were also used in evaluating the

effect of Clorox and pineapple waste with or without salt as bleaching salt. The effect

of the different solutions as whitening agents could not be evaluated because different

stains smeared after each treatment. Whitening of fabrics could not be visibly detected as

shown in Annex C. Result of Kramer’ rank sum test (Table 4) shows that there was no

significant preference between thetwo stain removal solutions when treated on grease and ink.

However, the same Table shows that there wasa significant preferenceon hair dye treated with

Clorox, deodorant treated with pineapple extract with salt, mud,and rust treated with pineapple

extract without salt.

 Table 4. Ranking on stain most preferred bleach

Stains Treatments Statistical

Chlorox Pineapple Pineapple Result
waste extract waste extract Kramers rank
with salt without salt sum test
5% 32 – 48
1% 30 - 50
grease (.01g) 32 40 48 non-
significant
ink (.01g) 35 38 47 non-
significant
hair dye (.01g) 30* 48 42 significant
deodorant (4 strokes) 50 27* 43 significant
mud (.01g) 44 46 30* significant
rust (.01grams) 37 53* 30* significant
Legend: * significant at 1% level(the least value with asterisk show the most preferred
bleached fabric, the highest value with asterisk showed the least preferred in
terms of whitening of fabric)

CONCLUSION

There wasa significant preference forthe effects of Clorox, pineapple waste with and without

saltbetween and among samples for all stains. The mostpreferredfor stain removal for grease,

ink and hair dye was the Clorox, deodorant- pineapple waste extract with salt, and mud and rust

– pineapple without salt.As fora whitening agent, panelist did not find any difference in fabrics

with grease and ink. However, panelists significantly preferred Clorox as a bleaching agent on

fabrics with hair dye, pineapple extract with salt on fabric with deodorant, and pineapple extract

without salt on fabrics with mud and rust.

RECOMMENDATION

1. The formulation and procedure should be disseminated as a livelihood project specifically

in the areas where pineapple abounds. However, more trials should be conducted to

further validate results.

2. Other stains should be tested with the pineapple waste extract.

3. Further study should be conducted on the reasons of the reactionbetween and among

basic solutions(Clorox), acid (pineapple waste extract) and sodium chloride.

 4. Standardize time of treatment of stain removals. Method of washing should be further

improved and should be based on a scientific background, including the period of

exposure.

5. Monitor the shelf life of the pineapple waste extract.

6. TESDA students should develop their critical analysis in conducting other research and

development in the above areas.

REFERENCES:

AOAC Official Method 942.15, 19th ed., 2012

Chemical composition and sensory analysis of fresh pineapple juice ...

https://pdfs.semanticscholar.org/22e4/8ef77903bddd70871aadabdc62a1e84e85ba.pdf
retrieved on
Dec. 2017

Consumer Guide (ND) Stain Removing Chemicals - Stain Removal Tools | HowStuff
WorksRetrieved from https://home.howstuffworks.com › Home & Garden › Stain Removal
on Dec 2017.

Cleanipedia (ND) Stain removal tips for clothes retrieved from

https://www.cleanipedia.com/gb/ laundry/how-to-remove-stains-from-clothes-a-handy-
stain-removal-guide on Dec 2017

Halvorson, C (ND) Uses Of Vinegar: Doing Laundry ( Cleaning Colors and

Removing Stains) retrieved from https://home.howstuffworks.com/home-
improvement/household-
hints-tips/cleaning-organizing/uses-for-vinegar-doing-laundry-ga1.htm retrieved on Dec
2017

Halvorson, C (ND Uses for Salt: Doing the Laundry: A Guide to Stain Removal
retrieved from https://home.howstuffworks.com/home-improvement/household-
hints-tips/cleaning-organizing/uses-for-salt-doing-the-laundry-ga3.htm on Dec.
2017.

https://www.veolia.com/sites/g/files/dvc1131/f/assets/documents/2015/11/stain-
removal.pdf

https://www.veolia.com/sites/g/files/dvc1131/f/assets/documents/2015/11/stain-
removal.pdf
https://en.wikipedia.org/wiki/Oxalic_acid

Mendoza, K. (2016), Feasibility of Pineapple (Ananas comosus) as a Stain Remover,

Retrieved from https://www.scribd.com/document/329722294/Feasibility-of-Pineapple-
docx
On Dec. 2017.

Remove stains: Stain of any sort yield to scientific treatment (ND) retrieved from
https://drive.google.com/file/d/1nCIv905aZGb7hpaA14AcppSZzcIo4MZg/viewlocate and
include

Sweet, D. (2012), Surfactant replacement. The newborn lung: neonatology questions and
controversies (second edition).

Stambolov, T. 1968 Notes on the removal of iron stains from calcareous stone Studies in
Conservation Vol 13, 1968 Issue 1 pp 45-47 https://www.tandfonline.com/doi/
abs/10.1179
/sic.1968.004?journalCode=ysic20 on 2014

Susan Rahfield, Pine Brook; Benjamin Newman, Elizabeth, both of N.J. Assignee: Boyle-
Midway Household (ND), Rust removal and method of use thereof for household
products, United States Patent (19) Rahfield et al. 54) (75) 73 (21) 22 51 52) (58) (56)
retrieved from https://patentimages.storage.
googleapis.com/ae/a3/9f/08d6ccf93d1912/US4828743.pdf

Veola (ND) retrieved

fromhttps://www.veolia.com/sites/g/files/dvc1131/f/assets/documents/ 2015/11/stain-
removal.pdf on Dec. 2017

What is the pH of pineapple juice and what is its color on the pH. Retrieved from
https://www.quora.com/ What-is-the-pH-of-pineapple-juice-and-what-is-its-colour-on-the
on Dec. 2017.

Young, J. (2017) What Type of Acid Is in a Pineapple? retrieved from

https://www.livestrong. com/article/282662 -dried-pineapple-nutrition/ on Dec.
2017
 Annex A

Forms Used in Conducting Ranking Preference Test on Stain Removal

UTILIZATION OF PINEAPPLE WASTES EXTRACT AS FABRIC STAIN REMOVER

Name of Panelist (Optional): _______________________________ Date:

_____________________
Sex: ________ Age: _______
Instructions:

Please evaluate and use the appropriate scale below to rate your preference on the extent of
stain removed.

STAIN __________

201 202 203

Comments:
____________________________________________________________________________

Rating Scale:1 - The most stain removed 2 - Stain moderately removed 3 - The less
stain removed
 Annex B

Forms Used in Conducting Ranking Preference Test on Whitening the Fabric

UTILIZATION OF PINEAPPLE WASTES EXTRACT AS BLEACHING AGENT

Name of Panelist (Optional): _______________________________ Date:

_____________________
Sex: ________ Age: _______

Instructions:

Please evaluate the whole fabric and use the appropriate scale below to rateyour preference on
the whitest cloth. Evaluate the wholefabric.

STAIN _____________

201 202 203

Comments:
____________________________________________________________________________

Rating Scale: 1 – Whitest 2 - Moderately white 3Least white

 Annex C

Photo Documentation on the Effect of Chlorox, Pineapple Waste Extract With or

Without Salt

Figure 1a. Effect of chlorox, pineapple extract with and without salt on grease, ink and hair dye
Figure 1b. Effect of chlorox, pineapple extract with and without salt on deodorant, mud,and rust