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    Biochem Experiment (Sakaguchi Test)

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    Camilogs
    The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

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    Biochem Experiment (Sakaguchi Test)

    Uploaded by

    Camilogs
    713 views2 pages
    The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

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    © © All Rights Reserved

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    The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

    Copyright:

    © All Rights Reserved
    713 views2 pages

    Biochem Experiment (Sakaguchi Test)

    Uploaded by

    Camilogs
    The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

    Copyright:

    © All Rights Reserved
    of 2

    SAKAGUCHI TEST

    OBJECTIVES:

    To detect amino acid guanidinium group like arginine.

    MATERIALS:

    APPARATUSES TO MATERIALS TO SAMPLES TO BE TESTED:


    BE USED: BE BROUGHT:

     3 Test tubes N/A  1 mL of 1% arginine + 2 drops of a-naphthol +


     1 Graduated 2 mL sodium hypochlorite + 1 mL of urea
    Cylinder solution
     1 mL of 1% glycine + 2 drops of a-naphthol + 2
    mL sodium hypochlorite + 1 mL of urea
    solution
     1 mL of 5% egg white (albumin) + 2 drops of
    a-naphthol + 2 mL sodium hypochlorite + 1 mL
    of urea solution

    PROCEDURE:

    1. Put 1mL of 1% arginine, 1 mL of 1% glycine, 1 mL of 5% egg white (albumin) in to


    different dry test tubes.
    2. Add 2 drops of α-naphthol to the three samples and mix well.
    3. Now add 2 mL sodium hypochlorite to all test tubes.
    4. Immediately add 1mL of urea solution to establish the red complex formed.

    DATA:
    QUESTIONS FOR RESEARCH:

    1. What is the principle involved in the Sakaguchi Test?

    2. How was the arginine able to condense with the alpha-naphthol (a-naphthol) to
    give a red solution?

    3. What is the significant of Sakaguchi Test?

    4. What are the chemical characteristics of amino acids? How does this affect
    protein structure?

    5. What is guanidinium group?

    REFERENCES:

    http://www.onlinebiologynotes.com/sakaguchi-test-objective-principle-reagents-
    procedure-and-result/

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