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Members List of Duties: Worksheet No. 2 Acetone Objective

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Members List of Duties

Agoto, Maria Victoria DT Sodium bisulfite test, Q5


Parungao, Ethyl Joy I. B. Results, Q2
Respicio, Frances Angelica 2,4-Dinitrophenylhydrazine test, Q3
Gabrang,Rogina Wenn I.A. Results,Q1, Schematic Diagram
Velano, Danica Sodium nitroprusside test, Q4, Schematic
Celestial, Floribel Conclusion

Worksheet no. 2
ACETONE

Objective:
To be able to detect the presence of Acetone

Materials:
Aspirator Stirring rod
Beaker Test tubes
Condenser Test tube brush
Distilling flask Test tube holder
Micropipette
Pipette

Reagents:
0.5% Sodium nitroprusside Mercuric iodide
Acetic acid Potassium iodide
Acetone KOH solution

Procedure:
I. Prepare all the materials needed
II. Perform the different tests for the detection of acetone

I. Detection using recorded video:


A. Place 1 mL of sample in a test tube. Add a few drops of freshly prepared alkaline solution of
sodium nitroprusside. Observe for the formation of color. Add excess acetic acid and observe
for the color change

RESULTS: ​Legal’s Test: Red to Orange solution and Greenish color (when heated)
B. Place the sample in a flask and add Nessler’s reagent (1:1). Distill and observe for the formation
of precipitate.

RESULTS: Lieben’s Iodoform Test: Yellowish white precipitate


Reynold’s Test: Black Zone

Create a schematic diagram of the detection tests performed in the laboratory experiment videos.

Legal’s Test Lieben’s Iodoform Test


II. Detection using simulator:

Test for Ketone

Detection Test Result

2,4-Dinitrophenylhydrazine test Formation of a Yellow


or Orange precipitate.

Sodium nitroprusside test Red-colored complex

Sodium bisulfite test White crystalline formation

Conclusion​:
Acetone is a chemical used to make household products. Our body also makes this
chemical when it breaks down fat. Based on the experiments conducted it is shown that
Acetone can be detected by multiple detection tests that require different reagents and
techniques. They give different positive results, however the presence of acetone can still be
concluded from these results.

PART II: Research Questions


In this part of the worksheet, answer the following guide questions related to Acetone. Limit your
answer to 5-7 sentences. Support your answer with valid and reliable references.

Questions:

1. Describe the acute and chronic effects of acetone to one’s health.

- ACUTE HEALTH EFFECT​ (short term)

1. Skin Irritation

2. Eye,nose & throat Irritation

3. Nausea & Vomiting

4. Headache
- CHRONIC HEALTH EFFECT​ (long term)

1. Cancer hazard

2. Reproductive hazard

3. May affects liver & kidney

Reference:
NJ Health. (2015). Right to know Hazardous substance fact sheet. Available at:
https://www.nj.gov/health/eoh/rtkweb/documents/fs/0006.pdf

2. Is the acetone used in nail salons the same with acetones available in the industrial field?
Explain.

- Acetone, a colorless liquid also known as Propanone, is a solvent used in manufacture


of plastics and other industrial products. Acetone is a solvent, which is a liquid that can
dissolve other substances. Acetone is most commonly known as the solvent that
removes nail polish, but it is also found in bath and fragrance products, hair and skin
care products, as well as skin-lightening products.
- When you touch nail polish removers that have acetone in them, you may notice that
your skin becomes very dry. Acetone is volatile. In other words, it evaporates quickly.
Acetone may also be used to where it's most frequent application would be in the
formulation of nail polish removers.
- Acetone is a primary ingredient in many nail polish removers. It breaks down nail
polish, making it easy to remove with a cotton swab or cloth. It is widely used because
it can easily mix with water and evaporates quickly in the air. Acetone is widely used in
the textile industry for degreasing wool and degumming silk.

Reference:
Garcia, N. (2015). What is Acetone? - Structure, Uses & Formula.Available at:
https://study.com/academy/lesson/what-is-acetone-structure-uses-formula.html​.
3. What happens inside the body of a person that consumed acetone?

- Consuming a small amount of acetone will not hurt a person. Thus, breathing a
moderate to large amount for a short period of time can irritate the nose, throat, lungs
and eyes of a person. It can also cause the person to feel uncomfortable by means of
headache, dizziness, nausea and vomiting effect, a faster pulse is felt, passing out and
possible coma. In women it can also cause a shorter menstrual cycle and birth defect.
In men, male reproductive problems were other results. A long term exposure to
acetone can damage the kidney, liver and nerves.

Reference:
Delaware Health and Social Services. (2015). Frequently Asked Questions: Acetone. Available at:
https://dhss.delaware.gov/dhss/dph/files/acetonefaq.pdf
4. How is acetone poisoning treated? Describe per route of exposure.

- Acetone poisoning can occur when there’s an abnormally high amount of ketones. This
is a condition known as ketoacidosis. Hundreds of commonly used household products
contain acetone, including furniture polish, rubbing alcohol, and nail polish.

Route of exposure:

Inhalation – overexposure to specific containing acetone.

Oral - accidentally drinking solutions that contain acetone

Reference:
The Merck Index. (2013). Acetone Monograph 65, O'Neil: The Royal Society of Chemistry. 15th Ed.
Available at: ​http://www.rsc.org/Merck-Index/monograph/mono1500000065

5. Can acetone poisoning cause death? Explain

- Taking in acetone in small quantities is likely to have little effect as your liver enzymatic
cytochrome p450 system detoxifies ketones, thus has the ability or capability to remove
significant amounts of acetone naturally. If the enzymatic breakdown process of
ketones by the liver is overwhelmed or compromised acetone poisoning will occur. If
the acetone poisoning is severe enough a deep stupor, coma or death may occur.

Reference:
Iceman, R. (2021). What happens if you drink nail polish remover? Available at:
https://www.quora.com/What-happens-if-you-drink-nail-polish-remover

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