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    Lactobacillus casei is grown under anaerobic conditions in a 40,000 L fed-batch fermenter operated at quasi steady-state over 6 hours with a feed rate of 4,000 L/hr and substrate concentration in the feed of 80 g/L. The initial culture volume is calculated to be 16,000 L. The substrate concentration at quasi steady-state after 6 hours is calculated to be 0.06 g/L. The cell concentration at quasi steady-state is calculated to be 18.39 g/L. The total mass of cells produced after 6 hours is calculated to be 729.3 kg.

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    Midterm2 PDF

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    Lactobacillus casei is grown under anaerobic conditions in a 40,000 L fed-batch fermenter operated at quasi steady-state over 6 hours with a feed rate of 4,000 L/hr and substrate concentration in the feed of 80 g/L. The initial culture volume is calculated to be 16,000 L. The substrate concentration at quasi steady-state after 6 hours is calculated to be 0.06 g/L. The cell concentration at quasi steady-state is calculated to be 18.39 g/L. The total mass of cells produced after 6 hours is calculated to be 729.3 kg.

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    Lactobacillus casei is grown under anaerobic conditions in a 40,000 L fed-batch fermenter operated at quasi steady-state over 6 hours with a feed rate of 4,000 L/hr and substrate concentration in the feed of 80 g/L. The initial culture volume is calculated to be 16,000 L. The substrate concentration at quasi steady-state after 6 hours is calculated to be 0.06 g/L. The cell concentration at quasi steady-state is calculated to be 18.39 g/L. The total mass of cells produced after 6 hours is calculated to be 729.3 kg.

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    89 views3 pages

    Midterm2 PDF

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    Trường Tùng Lý
    Lactobacillus casei is grown under anaerobic conditions in a 40,000 L fed-batch fermenter operated at quasi steady-state over 6 hours with a feed rate of 4,000 L/hr and substrate concentration in the feed of 80 g/L. The initial culture volume is calculated to be 16,000 L. The substrate concentration at quasi steady-state after 6 hours is calculated to be 0.06 g/L. The cell concentration at quasi steady-state is calculated to be 18.39 g/L. The total mass of cells produced after 6 hours is calculated to be 729.3 kg.

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    CM4710 ; Biochemical Processes

    2nd Mid-Term Examination


    October 26, 2001

    1. Fed-Batch Production of a Cheese Starter Culture (Doran, 1995). (54)


    Lactobacillus casei is grown under essentially anaerobic conditions to provide a starter
    culture for manufacture of Swiss cheese. The system has the following characteristics:

    YX/S = 0.23 (g dry cell wt./L)/(g substrate/L)


    KS = 0.15 g/L
    µmax = 0.35 hr-1

    A stirred fermenter is operated in fed-batch mode at quasi steady-state (concentrations do not


    change with respect to time in the reactor) with a feed flow rate of 4,000 L/hr and feed
    substrate concentration of 80 g/L. After 6 hr, the liquid volume is 40,000 L.

    a) What is the initial culture volume?


    b) What is the concentration of substrate at quasi steady-state at 6 hr?
    c) What is the concentration of cells at quasi steady-state at 6 hr?
    d) What mass of cells is produced after 6 hr of fed-batch culture, if the initial cell
    concentration prior to fed-batch culturing is 18 g/L?

    Fed -Batch Reactor

    F = 4,000 L/hr

    S o = 80 g /L
    Xo = 0 g/L
    S X

    V = rea ctor liquid volume a t any time

    Relevant Equations:
    µ max S
    Monod Specific Growth Rate: µ=
    KS + S

    CM4710 Biochemical Processees 1


    dV
    Volume: = F ⇒ V = Vo + Ft
    dt

    d(XV) dX dV
    Biomass: FX o + VµX = = V +X
    dt dt dt
    dV 1 dV F
    VµX = X ⇒ µ = = = D
    dt V dt V
    F F Do
    µ = = =
    V Vo + Ft 1 + Do t
    V µX d(SV) dS dV
    Substrate: FSo - = = V +S
    YX / S dt dt dt
    V µX dV
    FSo - = S
    YX / S dt

    a) dV/dt = F, or V=Vo+Ft. Solving for Vo, Vo = V-Ft = 40,000 L – 4,000 L/hr (6 hr) = 16,000

    b) µ = D at quasi steady-state
    µ max S
    = D ⇒ µmax S = KSD + SD
    KS + S
    K SD
    S( µmax - D) = KSD ⇒ S =
    µ max - D
    4,000 L / hr
    (0.15 g / L)( )
    40,000 L
    S = = 0.06 g / L
    -1 4,000 L / hr
    (0.35 hr - )
    40,000 L

    CM4710 Biochemical Processees 2


    c)
    VµX dV V µX
    FSo - = S ⇒ F(So - S) =
    YX /S dt YX /S

    µX YX / SD(So - S)
    D(So - S) = ⇒ X =
    YX / S µ
    but D = µ therefore
    X = YX /S (So - S) = (.23 g cells / g substrate)(80 -.6) g substrate / L = 18.39 g cells / L

    d) Xt = X(initial)Vo + X(V-Vo) = (18 g/L)(16,000 L) + (18.39 g cells/L)(40,000-16,000 L)

    = 729,268 g cells or 729.3 kg cells

    CM4710 Biochemical Processees 3

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