Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                

Colorimetry

Download as pdf or txt
Download as pdf or txt
You are on page 1of 2

Colorimetry/Spectrophotometry

We used colorimetry/spectrophotometry when we tested solutions to find the concentration for the
wastewater spill lab (in chem A) or in the patient prognosis/urine analysis lab (in chem B). We prepared
a set of 5 standard solutions, each with a different concentration. Then we put the five solutions in
cuvettes (which looked like small square test tubes). We put the cuvettes in the colorimeter, which was
a black plastic device that was hooked up to your Chromebook, and we used Graphical Analysis to
measure the absorbance of each solution.

This picture shows the general set-up of a coloimeter. Light with a specific wavelength is shined through
a solution in a cuvette. Some of the light is absorbed (“absorbance”), and some of the light is allowed to
pass through. (Absorbance is the amount of light that is absorbed, or blocked by a solution. The higher
the concentration, the higher the absorbance.)

After measuring the absorbance of the five solutions we had prepared, we also measured the
absorbance of a solution with an unknown concentration. Then we graphed the five standard solutions.
Assume that the graph below represents your five standard solutions in the lab. If the unknown has an
absorbance of 0.5, what is the approximate concentration? (Look on the next page to see the answer.)
Answer at bottom of page. Don’t scroll down until you’ve tried to answer the question on your own!

Answer to question on previous page: The concentration of the unknown is approximately 2.5 g/L.

You might also like