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Enzyme in The Leading Strand in The Lagging Strand: DNA Helicase

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Enzyme In the Leading Strand In the Lagging Strand

DNA Helicase Catalyses the unwinding of parental double helix at the


replication forks

Topoisomerase Catalyses the relieve of the strain due to the unwinding


process // prevent supercoiling on DNA double helix

Single-strand binding Bind to & stabilizes and single-stranded DNA until it


protein can be used as a template

Primase Catalyses the synthesizing Catalyses the elongation of


of single RNA primer at each Okazaki fragment
the 5’ end of the leading
strand

DNA Polymerase III Catalyses the continuation Catalyses the elongation of


of synthesizing the leading each Okazaki fragment,
strand, adding on to the adding on to its primer
primer

DNA Polymerase I Catalyses the degradation Catalyses the degradation


and replacement of RNA and replacement primer
primer with DNA with DNA nucleotide
nucleotide

DNA Ligase - Catalyses the annealing /


binding of the Okazaki
fragment

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