Experiment No. 2 PH and Buffer

Experiment no.
2
PH AND BUFFER
I. DATA AND RESULTS

A. Determine the pH of the following samples using a pH paper
1. Fresh milk- pH 6.5 and 6.7 7. Shampoo- pH 3.5- 9.0

2. Freshly voided urine- pH 6 8. Soap solution- pH 9-10
3. Vinegar- pH 2.5 9. Detergent solution- pH 7.0
4. 7-up- pH 3.79 10. Rubbing alcohol- pH 8
5. Coffee- pH 4.5 – 6.0 11. Agua Oxigenada- pH 4.5
6. Chlorox- pH 12.6 12. Tea- pH 7-1
B.
COMPUTATIONS:
 Ph Paper
1. Fresh Milk [H+]= antilog - [12]
pH= -log [H+] [H+]= 1 x 10 -12
[H+]= antilog - pH
[H+]= antilog - [7] 7. Shampoo
[H+]= 1 x 10 -7 pH= -log [H+]
[H+]= antilog - pH
2. Urine [H+]= antilog - [5]
pH= -log [H+] [H+]= 1 x 10 -5
[H+]= antilog - pH
[H+]= antilog - [5] 8. Soap Solution
+ -5
[H ]= 1 x 10 pH= -log [H+]
[H+]= antilog - pH
3. Vinegar [H+]= antilog - [7]
pH= -log [H+] [H+]= 1 x 10 -7
[H+]= antilog - pH
[H+]= antilog - [2] 9. Detergent Solution
[H+]= 0.01 pH= -log [H+]
[H+]= antilog - pH
4. 7-up [H+]= antilog - [10]
pH= -log [H+] [H+]= 1 x 10 -10
[H+]= antilog - pH
[H+]= antilog - [3] 10. Rubbing Alcohol
[H+]= 1 x 10 -3 pH= -log [H+]
[H+]= antilog - pH
5. Coffee [H+]= antilog - [6]
pH= -log [H+] [H+]= 1 x 10 -6
[H+]= antilog - pH 11. Agua Oxigenada
[H+]= antilog - [5] pH= -log [H+]
[H+]= 1 x 10 -5 [H+]= antilog - pH
6. Chlorox [H+]= antilog - [4]
pH= -log [H+] [H+]= 1 x 10 -4
[H+]= antilog - pH
12. Tea [H+]= antilog - [5]
pH= -log [H+] [H+]= 1 x 10 -5
[H+]= antilog - pH
 pH Meter
1. Fresh Milk
pH= -log [H+] 6. Chlorox
+
[H ]= antilog - pH pH= -log [H+]
[H+]= antilog - [6.4] [H+]= antilog - pH
[H+]= 3.98 x 10 -7 [H+]= antilog - [11.6]
[H+]= 2.51 x 10 -12
2. Urine
pH= -log [H+] 7. Shampoo
[H+]= antilog - pH pH= -log [H+]
[H+]= 5.01 x 10 -6 [H+]= antilog - [6.4]
[H+]= 3.98 x 10 -7
3. Vinegar
pH= -log [H+] 8. Soap Solution
[H+]= antilog - pH pH= -log [H+]
[H+]= 0.79 x 10 -7 [H+]= antilog - [8.6]
[H+]= 2.51 x 10 -9
4. 7-up
pH= -log [H+]
[H+]= antilog - pH 9. Detergent Solution
+
[H ]= antilog - [3.4] pH= -log [H+]
[H+]= 9.8 x 10 -4 [H+]= antilog - pH
[H+]= antilog - [10.6]
5. Coffee [H+]= 2.51 x 10 -11
pH= -log [H+]
[H+]= antilog - pH 10. Rubbing Alcohol
[H+]= antilog - [5.6] pH= -log [H+]
[H+]= 2.51 x 10 -6 [H+]= antilog - pH
[H+]= antilog - [6.4] [H+]= 2.51 x 10 -6
[H+]= 5.01 x 10 -3
12. Tea
11. Agua Oxigenada pH= -log [H+]
pH= -log [H+] [H+]= antilog - pH
[H+]= antilog - pH [H+]= antilog - [7.0]
[H+]= antilog - [5.6] [H+]= 1 x 10 -7
C. BUFFERS
II. Questions for Research

1. What is the normal pH of blood? Milk? Urine? Tabulate your answer.
2. What is the biochemical relevance of pH?

 pH is biologically important because it affects the structure and activity of macro-
molecules. pH is important in homeostatic processes. For example, most animals
breathe not because they lack oxygen, but because CO2 buildup in the blood increases
the blood acidity beyond normal levels.
3. Which sample in procedure B exhibited buffer action? Why?
 Sodium acetate, because buffer solutions resist a change in pH when small amounts of a
strong acid or a strong base are added. A solution of acetic acid and sodium acetate is
an example of a buffer that consists of a weak acid and its salt.
4. Compare the buffer systems in whole blood and blood plasma. Tabulate your answer
5. Give the Henderson-Hasselbach equation and give its importance in the study of
buffers
 The Henderson-Hasselbalch equation is useful for estimating the pH of a buffer solution and
finding the equilibrium pH in an acid-base reaction. The equation can be used to determine
the amount of acid and conjugate base needed to make a buffer solution of a certain pH.
III. References
https://www.msdmanuals.com/home/hormonal-and-metabolic-disorders/acid-base-
balance/overview-of-acid-base-balance#:~:text=The%20pH%20scale%2C%20ranges
%20from,of%20blood%20close%20to%207.40.
https://www.healthline.com/health/ph-of-milk#:~:text=types%20of%20milk-,Cow's
%20milk,because%20it%20contains%20lactic%20acid.
https://www.ncbi.nlm.nih.gov/books/NBK470195/#:~:text=Normal%20urine%20pH%20is
%20slightly,range%20is%204.5%20to%208.0.
https://en.wikibooks.org/wiki/Structural_Biochemistry/pH#:~:text=pH%20is%20biologically
%20important%20because,blood%20acidity%20beyond%20normal%20levels.
https://opentextbc.ca/chemistry/chapter/14-6-buffers/#:~:text=Buffer%20solutions%20resist
%20a%20change,weak%20acid%20and%20its%20salt.
https://microbenotes.com/henderson-hasselbalch-equation/
https://courses.lumenlearning.com/introchem/chapter/the-henderson-hasselbalch-
equation/#:~:text=The%20Henderson%2DHasselbalch%20equation%20is,in%20an%20acid
%2Dbase%20reaction.&text=The%20equation%20can%20be%20used,solution%20of%20a
%20certain%20pH.

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Experiment No. 2 PH and Buffer

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Experiment no.

I. DATA AND RESULTS

1. Fresh milk- pH 6.5 and 6.7 7. Shampoo- pH 3.5- 9.0

II. Questions for Research

2. What is the biochemical relevance of pH?

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