CL-32S/32L 40S/40M/40L: Instruction Manual

QD-200-P126-R2
AUTOCLAVE
(Steam Sterilizer)
CL-32S/32L
40S/40M/40L
Instruction Manual
● To use this product safely, make sure to read this manual.

● Place this manual in a convenient location for easy reference.
Contents
1. Preliminary notes P.3
2. For the safety use P.4～7
3. Part names P.8～9
4. Installation P.10～14
5. Operation method P.15～34
6. Trouble shooting P.35～37
7. Maintenance P.38～43
8. Specifications P.44
Appendix
● Setting flow chart P.45

● Block diagram P.46
2
１．Preliminary notes
● Thank you for choosing ALP products.

Keep this manual at your hand for your quick reference when operating this autoclave.
● Do not use this autoclave for improper purpose. Strict observance of this manual is required.
● Please do understand and observe safety instructions written in this manual.

Failure to follow safety instructions may cause trouble and / or injury.
● Following documents are attached with this manual. Please check and if some of them are
missing, contact local distributor.
・ Instruction Manual
・ Certificate of Quality
● The lid is not opened until putting the power on. Slide lid lock lever to open position
(right end) after power on. Install this autoclave according to instruction manual after
opening lid and unloading all contents inside chamber.
● Following standard accessories are included in this autoclave. If some of them are missing,
contact local distributor.
Base plate, 1pc.
Stainless wire basket

Stainless drain hose, 1pc. CL-32S : φ30×20cm 2pcs.
CL-32L : φ30×30cm 2pcs.
CL-40S : φ38×20cm 2pcs.
Exhaust bottle(4L), 1pc.
CL-40M : φ38×30cm 2pcs.
CL-40L : φ38×40cm 2pcs.
Base plate hook, 1pc.

(CL-40L only)
3
２．For the safety use
Preamble
Autoclave is being operated with high temperature and high pressure. Wrong installation
and /or failure to observe safety operation according to the instruction manual may cause
death or injury of operator and / or person near the autoclave, and / or destruction of
things.
Please read this chapter carefully then operate this autoclave. Important statements are
illustrated as follows. Please understand each meaning and observe these cautions.
The meaning of each illustration is as follows;
WARNING ：Ignore this warning may cause death or serious injury of person.
CAUTION ：Ignore this caution may cause injury and / or destruction of things.
Prohibited
Strictly Observed
4
● How to use autoclave
WARNING
This autoclave sterilizes objects with high temperature and high pressure.
Do not operate putting following materials in chamber.
・ Explosive materials
・ Flammable materials
・ Acid or Alkaline materials
・ Corrosive materials
・ Non heat-resistance materials
In case of sterilization of saline objects such as seawater, discharge saline water after
sterilization. Then put fresh water and operate several times.
Failure to do it may be triggered explosion by stemming of corroded chamber and pipes.
Do not disassemble and / or modify autoclave.
Autoclave being pressure vessel, disassemble and / or modification of autoclave may cause accident and / or
breakage of itself.
Do not move autoclave holding lid nor push it hard.
Lid may jolt out of alignment which leads to serious accident and / or trouble.
CAUTION
Do not get autoclave wet.
If electric component get wet, it may be broken, get a shock, and even cause fire.
● Installation
WARNING
Do not use power strip.
If power cable is bundled or tangled and /or wrongly connected, these may cause fire and / or get a shock.
Protective earth conductor must be connected with protective earth terminal.
Connection with gas pipe or water pipe may cause explosion, get a shock and /or breakage.
Do not install autoclave in the place where chemicals such as flammable materials, explosive
materials, and /or corrosive gas are kept.
Install autoclave in above mentioned place may cause serious accident and/or trouble of autoclave.
CAUTION
This autoclave should be used indoor. Do not install outdoor.
There are many possibilities of malfunction and/or breakage of autoclave if it is used outdoor.
This autoclave must be installed on level and hard floor.
Operation without stability may cause malfunction and /or serious trouble.
5
● Notes on the operation
WARNING
Be sure to close lid to prevent pinching dusts before operation.
If there is something between lid and chamber which prevents lid from closing, operator may burn by steam
leaked from chamber.
Be sure to slide lid lock lever slowly to close position (left end) before operation.
If lock is not enough, operator can not start operation (Er-4 is shown). But if operator can start operation by
malfunction of detection part, lid may explode at high pressure which cause fatal accident or serious injury.
Do not touch autoclave more than necessary.
If operator touches autoclave more than necessary, it may cause serious accident.
Do not open drain valve during operation.
It may cause burn injury by blow-out of boiling water and / or steam, water lack, and/or malfunction.
Do not open lid forcibly after pressing START KEY.
Opening lid forcibly after pressing START KEY may cause fatal accident or serious injury.
Do not open lid until chamber pressure goes down to “0MPa” when operation completed or
operation interrupted.
Steam spouts out of chamber by residual pressure which may cause burning.
In case of sterilizing liquid objects, to avoid exclusive boil, objects must be taken out of chamber
after cooling down enough taking time properly and unload quietly without any impact.
Liquid objects keep high temperature after sterilization completion since temperature of liquid cool down slower
than temperature in chamber. Therefore extreme temperature change when unloading may cause high temperature
liquid spouting by which operator may burn.
In case of sterilizing liquid objects, metallic or heat-resistance glass container must be used.
A glass container without heat-resistance may be easy to break by extreme temperature change and operator may
be injured by this breakage.
In case of sterilizing liquid objects (EX. liquid media), do not close container with lid or bung which
has no breathability.
It may cause rise of pressure in container, because temperature in container cool down slowly. Especially glass
container is easy to explode if it is damaged.
Use a heat-resistance glove when operator unloads objects after operation completion.
If operator touches chamber and/or objects without heat-resistance glove, operator may burn.
Do not open drain valve when there is residual pressure in chamber.
It may cause burn injury by blow-off of boiling water.
Do not touch exhaust bottle and stainless drain hose during air evacuation, exhaust, and drain.
It may cause burn injury. Do not touch them till cooling down enough.
Objects (especially sterilization bag) must be put in stainless wire basket or stainless bucket when
sterilization.
If operator loads objects into chamber directly, it may cause stopping up pipes which may lead to serious accident.
CAUTION
Do not put too much object in chamber for sterilizing.
（Quantity of object to be put inside chamber shall be less than 60% of capacity of chamber）
Putting too much object may cause sterilization incomplete.
In case of sterilizing containers, do not aim their open mouths upward nor overlap them each other.
Residual air may cause sterilization incomplete.
Sterilization bag shall be used with 100mℓ～200mℓ water inside. And open its mouth widely.
Residual air prevents penetration of steam which may cause sterilization incomplete.
Genuine accessories and spare parts made by ALP Co., Ltd. must be used.
Using other company’s products may cause serious accident and /or breakage of autoclave.
6
● Notes on power failure
In case of power failure during operation, autoclave works as follows.

(Refer to P.9 about LED, KEY, and DISPLAY)
1. Power to autoclave is lost, all controls stop.

2. After power is recovered, PROCESS LINE LED where it lighted up before power failure,
COOLING-2 LED, and COMPLETE LED light up.
However, TEMPERATURE DISPLAY shows current chamber temperature. In case of
sterilization, TIMER DISPLAY shows remaining time of sterilization, too.
3. Check the chamber temperature goes down below 79℃.
（Circumstantially, press EXHAUST KEY in order to shorten cooling time.）
4. After checking if pressure gauge indicates 0MPa, slide the lid lock lever slowly to open
position (right end) with pressing front part of the lid cover. Then, open lid softly.
5. Check the condition of PROCESS LINE LED.
If sterilization is incomplete, close lid and press START KEY after pressing STOP KEY.
(Refer to 5. Operation method)
WARNING
Do not open lid till pressure gauge indicates 0MPa and TEMPERATURE DISPLAY indicates below
79℃ when operation interrupted.
Be sure to set automatic exhaust OFF when sterilizing liquid object or glass container.
(Do not press EXHAUST KEY.)
Quick reduction of chamber pressure may cause breakage of glass container or blow-out of hot liquid.
Extra care about objects when unloading from chamber.
Or you may get burned or injured.
When sterilizing liquid, it takes a lot of time to cool. Be sure to check the temperature has dropped
sufficiently before unloading the liquid container from chamber.
Or you may get burned by bumping of liquid.
7
３．Part names
Main body
Lid cover
Lid lock lever
Operation panel
Lid hold
(both side)
Pressure gauge
Power switch
（Breaker）
Drain valve
Front caster with stopper
Safety valve
Stainless drain hose
Power cable
8
Operation panel 2 10 19 20 14 15 26 25 7 8 5 1
CLOSE OPEN
STERILIZE
O.T.
EXHAUST TEMP STOP
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER WARM PATTERN START
ENTER
HR MIN READY COMPLETE
3 11 18 13 12 21 22 16 17 23 24 6 9 4
1. Pressure gauge 15. EXHAUST LED

It indicates chamber pressure. The character blinks during exhaust process. To
2. TEMPERATURE DISPLAY exhaust after sterilization is displayed by the
It displays temperature in the chamber or setting character lighting-up.
temperature. Also, it displays the date of an operation 16. WARM LED
reservation. The character lights up during warming process. The
3. TIMER DISPLAY character blinks at warming setting change
It displays the remaining time to the process condition.
completion. “down count” Also, it displays the time of 17. COMPLETE LED
an operation reservation and error. The character lights up with the buzzer report in the
4. START KEY completion of all the processes. The character blinks
Press it to start operation. and reports an openable condition of the lid when
5. STOP KEY pressing STOP KEY on the way.
Press it to stop operation on the way. Press it to reset
previous operation before starting operation. PROCESS LINE LED
6. PATTERN/ENTER KEY They displays progress condition of the process by the
Press it to fix an operation pattern and each setting. extinction, the blink, and the lighting-up.
7. TEMP/TIME KEY 18. HEATING-1 LED
Press it to check setting temperature, setting time It blinks from start temperature of the sterilization
and the operation pattern. Press it to change setting or dissolving to 99℃.
display’s blink. 19. HEATING-2 LED
8. CHANGE▲ KEY It blinks from 100℃ to the sterilization
Press it to increase the figure of the setting display. temperature.
9. CHANGE▼ KEY 20. STERILIZATION LED
Press it to decrease the figure of the setting display. It blinks during sterilization.
10. EXHAUST KEY 21. COOLING-1 LED
Press it to switch automatic exhaust ON and OFF or It blinks to 100℃ after sterilization.
set an automatic exhaust starting temperature. Press 22. COOLING-2 LED
it to exhaust manually. It blinks from 99℃ after sterilization to the
11. START TIMER KEY completion.
Press it to make a reservation of operation. Set an 23. WARMING LED
operation starting time the month, the day, at the time, It blinks during warming.
and in the minute. 24． COOLING-3 LED
12. READY LED It blinks from 99℃ after warming to the completion.
The character lights up at the ready condition. The
character blinks at setting change condition. STANDARD OPTION FEATURE
13. HEAT LED 25. OBJECT TEMPERATURE KEY
The character lights up during heating or dissolving Connect the object temperature sensor of option
process. The character blinks at dissolving setting part. The object temperature can be checked with
change condition. pressing it. Keep pressing it, which enables
14. STERILIZE LED sterilization timer to start when object temperature
The character lights up during sterilizing process. The reaches to setting temperature.
character blinks at sterilizing setting change 26． OBJECT TEMPERATURE LED
condition. It lights up at the object temperature mode.
9
４．Installation
４．１ Transportation
This autoclave has casters. So it can move horizontally.
WARNING
Do not pinch hands / feet when moving autoclave.
There is a risk of a fracture or bruise.
CAUTION
Do not hit and /or get shock to autoclave nor hold lid cover.
It may cause breakage or damage of autoclave.
４．２ Installation location

Autoclave must be installed as follows.
Up to celling or duct
Refer to 4.3～4.6. Wall
surface
80cm or more
Wall
surface
20cm or more
20cm or more
10cm or more
Wall surface The end of stainless drain hose

or other must be led to heat-resistance
instruments drain facility or exhaust bottle.
Lock front casters
Level and hard floor
This autoclave has process of air and steam evacuation from inside chamber to outside
during operation. When you decrease chamber pressure forcibly, you need to exhaust steam
to outside. So installation location needs following facilities.
A. There is heat-resistance drain facility at installation location.
B. In case of lack of drain facility as A, use exhaust bottle.
10
WARNING
Do not install where explosive, flammable, and / or corrosive chemical agents are kept.
Serious accident and /or breakage of autoclave may happen if it is installed with above mentioned chemical
agents.
This autoclave must be installed to a place where operator can read pressure gauge.
Or it may cause malfunction and /or serious trouble.
CAUTION
This autoclave must be installed on level and hard floor.
Keep 10～20 cm space between autoclave and wall or other instruments when installation.
Install autoclave avoiding humid and dusty place and keep away from direct sunlight.
Lock stoppers of front casters.
Autoclave must not be installed outdoor.
Breakage of autoclave may happen if it is installed outdoor.
４．３ Installation condition
Autoclave must be installed under following condition.

・Ambient temperature : 5～35℃
・Relative humidity : 30～85％
Do not install autoclave locations as follows

・Outdoor
・Locations which receive direct sunlight
・Locations where autoclave get wet
・Dusty locations
・Sloping ground
・Locations with saline and /or sulfur in the air
・Locations except suggestible ambient temperature and relative humidity
・Locations over 1,000 meter's high above the sea
４．４ Power cable connection
Lid is not opened until putting the power on. Slide lid lock lever to open position (right end)
after power on. Then open lid and unload all contents inside chamber.
(Refer to 4.6 Standard accessories)
Do not press START KEY during this operation. Otherwise contents inside chamber may
burn out due to the water lack.
11
Please note following points when power connection.
・Be sure to use outlet and plug which meet the specifications.
（Refer to 8. Specifications）
・Be sure to check voltage and allowable current value of outlet. Failure to connect outlet
with proper voltage and allowable current value may cause breakage.
(Refer to 8. Specifications)
・Do not use power strip.
・When you connect power cable to distribution board directly, beware of faulty wiring and
electric shock.
WARNING
Power plug must be proper one to fit the connector.
Keep space between power cable and stainless drain hose.
Or it may cause burning of power cable which leads to breakage of autoclave.
Do not bend and / or bundle power cable.
Improper wiring may cause fire or power leakage due to bad electrical failure or malfunction.
Do not put heavy things on power cable.
Do not use power strip.
Do not touch power plug by wet hand.
Operator may get electric shock.
CAUTION
Power cable must be singly-connected to the commercial power with proper voltage and
amperage.
Improper connection may cause malfunction or trouble.
４．５ Protective grounding
Earth cable of the power cable must be connected to plug or ground terminal of distribution
board.
WARNING
Earth cable must be connected to earth terminal.
Do not connect earth cable to gas pipe, water pipe, telephone line or lighting rod.
Improper connection may cause fire or power leakage due to bad electrical failure or malfunction.
If connector with earth terminal is not provided, make the connector with earth terminal.
12
４．６ Standard accessories
① Turn on power switch, then slide lid lock lever to open position (right end) and open lid.
② Unload all standard accessories. (Refer to １．Preliminary notes)
③ Set every accessory as follows.
●Base plate・・・Put it on the bottom of chamber.

Purpose of use：To protect objects from direct heat generated by heater.
Level of base plate is also proper water level.
●Stainless drain hose・・・Set as figure 1.

Purpose of use：Discharge way of residual steam, air, and water.
Hitch ring to hook when needed
Connect the end of stainless drain hose
Figure 1 How to set stainless drain hose
In case of using heat-resistance drainage channel, lead end of stainless drain hose to
the drainage channel. (Refer to figure 2)
In case of using heat-resistance drainage pit, lead end of stainless drain hose to
the drainage pit. (Refer to the figure of 4.2 Installation location)
Lay the hose.
Figure 2 Case of using drainage channel
13
●Exhaust bottle・・・When there is not heat-resistance drain facility at installation location,
set as the figure of 4.2 Installation location.
Purpose of use：When there is not heat-resistance drain facility at installation location,
it is discharge destination of residual steam and air.
If you hitch ring of stainless drain hose to hook of main body, you can keep hose stand.
Then, extra care about follows.
・ Make end of stainless drain hose not to touch liquid level in drain facility or exhaust
bottle.
・ Do not use exhaust bottle for drain.
(Refer to 7.1 Daily maintenance about drain)
●Stainless wire basket・・・ Use it during operation.

Purpose of use : Load objects into it for operation.
●Base plate hook(CL-40L only)・・・Keep it with this instruction manual.

Purpose of use：Tool for unloading base plate from bottom of chamber at the time of
maintenance.
CAUTION
Be sure to use base plate during operation.
Operation without base plate may cause burnout of object.
Stainless drain hose must be screwed up tight.
If there is screw loose, it may cause leakage of steam and / or water from connection.
Lead end of stainless drain hose to heat-resistance drain facility or exhaust bottle, and keep
away from power cable and outlet as far as possible.
Do not use exhaust bottle for drain.
In case of using exhaust bottle, set backward of autoclave where a person does not lay it by
mistake.
Install autoclave in above mentioned place, or it may cause burn injury.
14
５．Operation method
WARNING
Be sure to read this chapter carefully before operation and use autoclave properly under
thorough understanding.
Or it may cause fatal accident at worst as well as mechanical trouble.
● Flow chart of basic operation
１. Turn on power switch(breaker)
２. Close drain valve
３. Pour water
４. Load object
５. Close and lock the lid
６. Press STOP KEY
７. Set operation pattern,

value of temperature and time
８. Press START KEY
９. Completion of operation
１０. Unload object

（below 80℃ in chamber）
１１. Close and lock the lid
１２. Turn off power switch(breaker)
15
Before operation
WARNING
In case of sterilization of objects such as electrolyte-containing saline (EX. sea water) and/or
object which generate sulfide and/or chlorine gas, be sure to discharge water in chamber then
clean it after sterilization completion.
Failure to maintain after sterilization may cause corrosion of bottom of chamber by electrolytic corrosion which
leads to rot.
Be sure to check if pressure gauge indicates “0MPa” when the lid is opened.
If pressure gauge does not work properly, please contact local distributor.
If pressure gauge is wrong, it may cause serious accident and/or breakage of autoclave.
End of stainless drain hose must not be choked up.
Or residual air remains in chamber, it may cause serious accident and /or breakage of autoclave.
CAUTION
Lead end of stainless drain hose to heat-resistance drain facility or exhaust bottle.
Or it may cause burn injury and / or breakage of autoclave by blow-off of boiling water and steam.
５．１ Turn on power switch(breaker)
① Check if power cable is connected properly.

② Power ON.
WARNING
Do not touch power switch(breaker) by wet hand.
Operator may get electric shock.
５．２ Close drain valve
① Stand the lever of drain valve to close drain valve.

(Check if the lever stands.)
CLOSE
OPEN
CAUTION
Be sure to close drain valve.
Or water leaks from chamber, it may cause water lack, malfunction and/or breakage of autoclave.
16
５．３ Pour water
① Check if pressure gauge indicates “0MPa”.

② Open lid and pour water to the same level as base plate.
Base plate Chamber
Proper level
Heater
WARNING
Do not open lid until chamber pressure goes down to “0MPa”.
CAUTION
Water level in camber is getting lower during operation. Check water level every operation and
pour water to the same level if it’s under proper level.
Water put in chamber must be distilled one or purified one. Frequent cleaning of chamber is
needed when using city water which generates scale in it.
Do not spill water when pouring it. If you spill water, wipe up it.
Change water for sterilizing frequently. Water must be changed every day in case of daily use.
Do not use well water which may cause corrosion and/or smudge.
Failure to use proper water may decrease life of heating element and/or chamber.
17
５．４ Load object
① Objects must be put in stainless wire basket or stainless bucket (optional accessory),
then set in chamber slowly.
※Do not put too much objects in chamber.

Too much objects in chamber may cause sterilization incomplete.
（Quantity of objects to be put inside chamber shall be less than 60% of capacity of
chamber）
WARNING
Be sure to use stainless wire basket, stainless wire basket with pan or stainless bucket.
In case of sterilization without above accessories, chamber pressure become out of control
due to the objects choke up pipe which leads to serious accident such as explosion.
Be sure that objects do not make contact with the inside of the lid.
If the lid is not closed properly, it may cause serious injury by blowing steam /or serious accident.
CAUTION
In case of sterilization of flagons such as vial container，screw cap bottle，conical flask, take out
cap or screw down it.
（Do not close mouth of flagons with impervious material such as silicon or rubber.）
In case of sterilization of liquid objects, quantity of object to be put inside container shall be less
than 50% of capacity of container.
Steam does not penetrate which may cause sterilization incomplete.
Too much object in container may cause bumping when objects are taken out of chamber after operation
completion.
18
●How to use the sterilization bag
①． To avoid breakage of sterilization bag, use with stainless bucket or stainless wire basket with pan
for sterilization. Don’t put the sterilization bag into chamber directly without using stainless
bucket or stainless wire basket with pan. Because exhaust holes located in the wall of chamber
may choke by sterilization bag which cause sterilization incomplete. In addition, pipes for control
and safety device may choke by sterilization bag which cause serious accident.
Be sure to put sterilization bag into stainless bucket or stainless wire basket with pan.
②． Don’t put too much objects into the sterilization bag. Quantity of object to be put in the
sterilization bag shall be less than 60% of chamber volume. Too much objects in the sterilization
bag may cause sterilization incomplete.
③． Sterilization bag must have open mouth. Sterilization bag must be set being open. (Refer to next
figure). If sterilization bag need to be tied with band, be sure to secure space for the steam going
out of sterilization bag. Closing the sterilization bag when sterilization may cause burst in addition
to the sterilization incompletion.
Close the bag when sterilization bag is disposed after sterilization.
④． For sure sterilization, it is advisable to put some water (about 200ml) into sterilization bag before
sterilization. Steam is generated in the sterilization bag which leads to better sterilization. In case
of sterilization without water in the sterilization bag, sterilization time must be set longer than in
case of sterilization with water.
⑤． If sharp pieces such as broken petri dish, test tube, and needles are directly put in the sterilization
bag, it may be cause of breakage of sterilization bag.（In case of using heat shrinkable sterilization
bag, it may break when a large quantity of hard materials such as disposable petri dishes are put
in the bag.）
⑥． It is difficult for linen and gauze to penetrate steam. They must not be put too much in
sterilization bag. This may cause sterilization incomplete.
⑦． Be sure to check the water level before every operation. In case of water lack, sterilization bag
may melt and stick to bottom of chamber by heat from heating element.
Open mouth during

sterilization. stainless wire basket with pan
※In case of using band,
open mouth as wide as Chamber
possible.
Sterilization bag
Objects
Stainless bucket
(Stainless wire Base Plate
basket with pan)
Water
(about 200ml) Heating element
19
※ Sterilization conditions may vary depending on the objects. Use the chemical indicator and so on to get
suitable sterilization condition for each object.
※ If above ②⑥ happen and/or failure to do ③④, following safety device may work due to over pressure
in chamber by residual air.
- Pressure safety valve (Steam evacuates out of chamber)
●How to use the stainless bucket
①． Do not stack stainless buckets up in chamber. If the space between buckets is not enough, the
sterilization may be incomplete as air convection in chamber is insufficient.
②． Load stainless bucket in the center of chamber. If the space between the bucket and the
chamber’s wall is not enough, there is a possibility that sterilization is incomplete as evacuating
air is bung up. Also overpressure may cause safety device working.
③． Put water (200ml) into stainless bucket.

The sterilization efficiency improves as steam is generated. The sterilization time shall be longer if
you sterilize without water.
④． Do not overload objects which are difficult to penetrate steam (linen, gauze, etc…), or it may
cause sterilization incomplete.
⑤． Do not put too much objects in chamber for sterilization.

(Quantity of objects to be put inside chamber shall be less than 60% of capacity of chamber.)
⑥． Sterilization time / temperature shall be longer/higher, as evacuating air's efficiency of

sterilization bucket is not better than stainless wire basket with pan. Use the chemical indicators
and so on to get suitable sterilization condition.
Ensure a space
Stainless bucket
Water
Chamber
(about 200ml)
Objects
Base plate
Heater
20
５．５ Close and lock the lid
① Grip lid hold and close lid softly.

② Slide the lid lock lever slowly to close position (left end) with pressing front part of the
lid cover.
①
CLOSE OPEN
WARNING
Be sure lid gasket has no dusts and crack.
Wipe out dusts on gasket with a soft cloth.
If you find crack on gasket, please contact local distributor.
If you leave crack one unchanged, it may cause burst at worst as well as leakage of steam.
Be sure to slide lid lock lever slowly to close position (left end) before operation.
If lock is not enough, operator can not start operation (Er-4 is shown). But if operator can start operation by
malfunction of detection part, lid may explode at high pressure which cause fatal accident or serious injury.
Do not slide the lid lock lever by force.
Forcing the lever to move may damage to mechanical locking device, it may cause unexpected accident.
CAUTION
Soon after previous operation (in case that TEMPERATURE DISPLAY indicates 50－79℃ after
opening lid), wait for a minute with closing lid, then slide the lid lock lever.
Reactive force arises when closing lid because the air which was caught by lid is swollen by heat of chamber.
Therefore, it’s necessary to keep the lid closing for a minute to discharge swollen air in chamber.
21
５．６ Press STOP KEY
① Press STOP KEY to reset previous operation process.
CAUTION
Be sure to press STOP KEY before setting and starting operation.
If previous operation process is not reset, you can not set and start operation.
５．７ Set operation pattern, value of temperature and time
5．7．1 Operation pattern setting

Holding pattern When press shift to
Sterilization When press shift to

1 0
2 2
1 0
Press PATTERN/ENTER KEY.
pattern
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN READY
Press CHANGE▲▼ KEY to shift

1 0
2 2
1 0
Sterilization/
Warming pattern to next operation pattern.
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Press PATTERN/ENTER KEY

7
0
Dissolving/
to set operation pattern.
Warming pattern
O
.
T
.
EXHAUST TEMP STOP

0
3
0
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Sterilization pattern
This operation pattern intends to sterilize contents.
Sterilization / Warming pattern
This operation pattern intends to sterilize and warm contents.
Dissolving / Warming pattern
This operation pattern intends to dissolve and warm agar medium or the like.
22
5．7．2 Temperature / time setting
①Set operation temperature and time in the range of below setting range.
(Refer to figure of next page too)
Setting range
Sterilization pattern
CL-32S/32L : Sterilization temp.：40℃ to 140℃
Sterilization time：0minute to 10hours or （continuous run）
CL-40S/40M/40L : Sterilization temp.：40℃ to 137℃
Sterilization/Warming pattern
CL-32S/32L : Sterilization temp.：40℃ to 140℃
Warming temp.：40℃ to 60℃
Warming time：0minute to 48hours
CL-40S/40M/40L : Sterilization temp.：40℃ to 137℃
Dissolving/Warming pattern
CL-32S/32L/40S/40M/40L :
Dissolving temp.：40℃ to 99℃
Dissolving time：0minute to 10hours or （continuous run）
●Continuous run setting
When setting sterilization time or dissolving time, press CHANGE▲ KEY at

10hours 00minutes. And you can set continuous run.
CAUTION
When object is a large amount of liquid or object is put in container which is difficult to
evacuate air, temperature of object rises slowly. In this instance, raise sterilization temperature
or lengthen sterilization time properly.
Use chemical indicator and so on to check sterilization condition for object periodically.
23
When press shift to
Holding pattern(in case of Sterilization / Warming pattern)
In case of other patterns, operate in the same way. When press shift to
2
5
Every pattern needs setting.
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
2
0
℃ TIME
MON DAY
START HEAT
ENTER
HR MIN
To the mode of
1 0
2 2
1 0
temperature / time setting.
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Sterilization temperature is
1 0
2 3
1 0
changed with CHANGE▲▼　KEY.

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Sterilization time is changed

6
0
with CHANGE▲▼　KEY.
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
1
2
0
0
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Warming temperature is
6
0

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
2
4
0
0
TIME
MON DAY
START HEAT
ENTER
HR MIN READY
Press PATTERN/ENTER KEY to set temperature/time. Warming time is changed with

CHANGE▲▼　KEY.
24
5．7．3 Exhaust setting
Automatic exhaust
This is the function that autoclave exhausts steam to outside automatically after sterilization
to cool down quickly. It’s necessary to set in each operation depending on objects as follows.
For appliance(except glassware) ・・・
For glassware, liquid or agar medium, other low viscosity substance ・・・
Additionally, automatic exhaust starting temperature can be set in concert with boiling point of
object to prevent boil over at automatic exhaust .
2 2
5 0
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
1 0
2 F
1 F
To the mode of exhaust setting
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN READY
Exhaust setting is 0N with CHANGE▲ KEY

0
F
F
1 0
2 N
1
and with CHANGE▼ KEY.

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN READY
The setting is fixed with PATTERN/ENTER KEY.

1 0
1 N
0
Exhaust starting temperature

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN READY
Exhaust starting temperature is The setting is fixed with PATTERN/ENTER KEY.

2 2
5 0
EXHAUST LED lighting-up

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
25
CAUTION
Set automatic exhaust in each operation depending on object which is sterilized.
The object which is insufferable under rapid pressure and temperature reduction may burst in chamber.
５．８ Press START KEY
Press START KEY to start operation.

① The heater is turned on electricity even as “the output lamp of heater” which on TEMPERATURE
DISPLAY lights up, and HEATING-1 LED blinks.
② Mixture of steam and air in chamber is evacuated from stainless drain hose with rise of temperature.
This phenomenon is the process to fill chamber with saturated steam, is not abnormal condition.
The mixture steam stops evacuating at 101℃-107℃ with closing exhaust valve which is controlled
by microprocessor.
TEMPERATURE DISPLAY indicates above 100℃, HEATING-2 LED blinks as below chart.
Subsequently, temperature and pressure in chamber rise in a matter of minutes, to get to setting
sterilization temperature.
③ When the temperature in chamber gets to setting temperature, sterilization timer starts to work, and
remaining time of sterilization is counted down on TIMER DISPLAY.
Subsequently, the autoclave keeps the temperature and pressure constant for remaining time.
STERILIZATION LED and the output lamp of heater blink during sterilization as below chart.
2 2
5 0
Press START KEY. Start operation.

①
The output lamp

O
.
T
.
of heater EXHAUST TEMP STOP

0
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
1 0
0 2
1 0
②
O
.
T
.
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
1 0
2 1
1 9
③
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
PATTERN
Remaining time of TIMER START
sterilization ENTER
HR MIN
WARNING
Do not touch autoclave more than necessary.
If operator touches autoclave more than necessary, it may cause serious accident.
26
５．９ Completion of operation
1 0
0 0
7 0
During exhaust
④
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
EXHAUST
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
8
⑤
0
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
0
0
EXHAUST pi,pi,pi,pi,pi,
TIME
℃ pi,pi,pi,pi,pi,
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN COMPLETE
Lighting-up:Lid lock can not be released yet.
Press STOP KEY.
Extinction :Lid lock can be released.
④ After sterilization time has passed, complete alarm sounds and power to heater is cut
off. In case of setting automatic exhaust ON as above chart, autoclave exhausts
steam by itself after sterilization process, and decrease pressure forcibly in chamber.
（In case of setting automatic exhaust OFF, EXHAUST LED does not blink and
autoclave does not exhaust steam by itself. But if you press EXHAUST KEY,
autoclave exhausts steam.）
COOLING-1 LED blinks from sterilization temperature to 100℃.
After that COOLING-2 LED brinks from 99℃ to completion.
⑤ When TEMPERATURE DISPLAY indicates 80℃, complete alarm sounds and
COMPLETE LED lights up.
⑥ Press STOP KEY to release the lid lock. Then the lid can be opened and COMPLETE
LED turns off.
※In case of interruption of operation, the lid can not be opened till TEMPERATURE DISPLAY
indicates below 80℃ too.
WARNING
Be sure to set automatic exhaust OFF when sterilizing liquid object or glass container.
(Do not press EXHAUST KEY.)
Quick reduction of chamber pressure may cause breakage of glass container or blow-out of hot liquid.
In case of breakage or spill of objects, be sure to clean inside chamber to use autoclave safely.
Or it may decrease life of heating element and/or chamber, then causes breakage of autoclave.
Do not slide the lid lock lever by force nor open lid till TEMPERATURE DISPLAY indicates below
80℃.
Lid lock can not be released. Because it is mechanically-locked. Forcing the lever to move may damage to
mechanical locking device, it may cause unexpected accident.
27
CAUTION
Be sure to press STOP KEY after operation completion to release lid lock.
If you do not press STOP KEY, you can not open the lid.
●Checking operation pattern, value of temperature and time during operation

2 2
5 0
O
.
T
.
EXHAUST TEMP STOP
0
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
Sterilization
1 0
2 2
1 0
temperature O
.
T
.
STERILIZE
EXHAUST TEMP STOP
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Warming Sterilization
5 0
0 0
temperature time
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
2
TIME
℃
MON DAY
START HEAT
ENTER
HR MIN READY
Warming time
EXHAUST LED lights up when setting automatic exhaust ON.

5 seconds passing, the display returns to previous indication.
Above chart bespeaks below setting values at sterilization / warming pattern.

Sterilization temperature 121℃
Sterilization time 20 minutes
Warming temperature 50℃
Warming time 2 hours
28
５．１０ Unload object (below 80℃ in chamber)
① Check if pressure gauge indicates 0MPa and TEMPERATURE DISPLAY indicates

below 80℃．
② Slide the lid lock lever slowly to open position (right end) with pressing front part of
the lid cover and open lid.
③ Unload objects slowly.
WARNING
80℃.
Or the liquid bumps out of container, you may get burned.
５．１１ Close and lock the lid
① Grip lid hold and close lid softly.

② Slide the lid lock lever slowly to close position (left end) with pressing front part of the
lid cover.
５．１２ Turn off power switch(breaker)
① Drain water in chamber with opening drain valve when autoclave is not used
for a long term. (Refer to 7.1 Daily maintenance)
② Turn off power switch(breaker).
WARNING
It may cause electric shock.
29
● Interruption of operation
Follow below steps to interrupt operation after pressing START KEY.

① Press STOP KEY and cool down the chamber.
（Circumstantially, press EXHAUST KEY in order to shorten cooling time.）
② When TEMPERATURE DISPLAY indicates 79℃, complete alarm sounds and
COMPLETE LED blinks.
③ Press STOP KEY again to release the lid lock. Then COMPLETE LED turns off.
④ After check if pressure gauge indicates 0MPa, slide the lid lock lever slowly to open
position (right end) with pressing front part of the lid cover and open lid.
WARNING
79℃.
Or the liquid bumps out of container, you may get burned.
● Pressure safety switch
Pressure safety switch is installed on this autoclave.

It works when overpressure in chamber due to sterilization bag being closed etc.
Power to the heater is cut to prevent pressure in chamber when it works.
30
● Safety valve
Safety valve is installed on autoclave as safety device against over pressure.

If chamber pressure increases beyond maximum operating pressure cause of some kind,
safety valve opens by itself, at once decrease pressure in chamber. If safety valve activates
in operation by any outbreaks, please act on the following procedure.
① Stay away from autoclave because of steam blowing.

② After losing steam, turn off power switch(breaker). Then Inside chamber cools
naturally.
③ Contact local distributor. When contacting the distributor, be sure to have model and
serial number information.
WARNING
Do not disassemble or adjust safety valve since it has been adjusted in order before shipping.
It may cause fatal accident because of abnormal performance.
５．１３ Other functions
Autoclave has calendaring function. It’s necessary to set date and time as follows according
to the situation.
５．１３．１ Date and time setting
Autoclave has already been adjusted local time which will be used before shipping. But if you
would keep turning off power switch among long term (more than a month), internal battery
discharges own storage, then calendar is reset to Jan. 1st, 2000 A.D. 0:00. (Er-6 indicated)
Set up current date and time as next page.
31
2
5
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
E
R
6
TIME
℃ pi,pi,pi,
MON DAY
pi,pi・・・
START HEAT
TIMER PATTERN START
ENTER
HR MIN
2
5
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
2
0
TIME
℃
MON DAY
START HEAT
TIMER PATTERN START
ENTER
HR MIN
Press START TIMER KEY To the mode of

2000 A.D.
2
0
0
0
for 5 seconds. date and time setting.

O
.
T
.
EXHAUST TEMP STOP
TIME
℃
MON DAY
START
TIMER PATTERN START
ENTER
HR MIN READY
Year is changed
1 0
1 0
with CHANGE▲▼ KEY.

O
.
T
.
EXHAUST TEMP STOP

0
TIME
℃
MON DAY
START
TIMER PATTERN START
ENTER
HR MIN READY
Month, day, hour, and minute are

8
2
9
They are stored with PATTERN/ENTER KEY.

O
.
T
.
EXHAUST TEMP STOP

1
5
1
5
℃ TIME
MON DAY
August 29th START
PATTERN START
3:15 pm TIMER
ENTER
READY
HR MIN
Press START KEY to

fix date and time.
32
５．１３．２ Reservation operation
Autoclave has function of reservation operation which can be set arbitrarily starting time of
operation with calendar timer. If you set reservation operation at previous day, you will
unload objects from chamber soon. This function brings about advancement of operating
efficiency. You can use it as following procedure circumstantially.
Perform 5.1～5.7 before reserving operation.
2 2
5 0
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
0
℃ TIME
MON DAY
START HEAT
ENTER
HR MIN
Current time is displayed.

8
3
1
August 31st. 4:46 pm To the mode of reservation
O
.
T
.
STERILIZE
EXHAUST TEMP STOP
1
6
4
6
℃ TIME
MON DAY
START HEAT
ENTER
HR MIN READY
Month, day, hour, and minute are

9 8
1 1
They are stored with PATTERN/ENTER KEY.

O
.
T
.
STERILIZE
EXHAUST TEMP STOP
5
℃ TIME
MON DAY
START HEAT
ENTER
HR MIN READY
September 1st. 8:15 am Press START KEY to start

98
11
Start of operation resevertion operation.

O
.
T
.
EXHAUST TEMP STOP

5
TIME
℃
MON DAY
START
TIMER PATTERN START
ENTER
HR MIN READY
CAUTION
Be sure to check drain valve is closed and water level in chamber before reservation operation.
There is a possibility that autoclave breaks down with water lack.
Be sure to check the condition of stainless drain hose destination.
There is a possibility that boiling water and steam may drain to unexpected places. Lead stainless drain hose to
proper place. (Refer to 4．2 Installation location)
33
５．１４ Reliability of sterilization
In case of sterilizing following containers which hinder the circulation and penetration of
steam, the temperature rises slower than temperature in chamber. Because residual air in
following containers is difficult to be evacuated.
Also it may cause Er-1(over pressure error).
●Containers which hinder the circulation and penetration of steam

・ Sterilization bag
・ Corking containers
・ Deep containers
・ Containers with comparatively small mouse
Especially, in case of using sterilization bag and stainless bucket, refer to 5.4 Load object to
prevent sterilization incompletion and/or danger of over pressure.
To operate assured sterilization, perform following ways.

・ Pour 100～200mℓ water into containers and generate steam to evacuate residual air.
・ Use indicators to check assurance of the sterilization.
・ Connect object temperature sensor of option part, then operate sterilization at the
object temperature mode.
This operation mode enables sterilization timer to start when object temperature
reaches to setting temperature. The object temperature can be checked with pressing
OBJECT TEMPERATURE KEY.
34
６．Trouble shooting
CAUTION
If the situation of autoclave correspond to following item(①or②) when you ask for repair
autoclave, make autoclave and accessories to be non-polluting condition.
① When in part of autoclave is exposed to infectious substance or radioactive substance.

② When blood or other hazardous components for human body adhere to part of autoclave.
When water lack alarm sounds, turn off power switch(breaker) and let stand for an hour because
the chamber is high temperature. And then pour water into chamber.
Or it may cause heater trouble.
In case of trouble on autoclave even if you operate correctly under this instruction manual,
check out the cause and suitable treatment according as follow table. If there are items which
do not correspond to follow table, or if it is difficult to treat, please contact local distributor
after turning off power switch(breaker) and noting serial number of the autoclave.
TROUBLE CONDITION CAUSES COUNTERMEASURES

A. Power is not on. 1. Power cable being disconnected. 1. Be sure to connect cable.
2. Misconnection of power cable. 2. Be sure to connect cable appropriately.
3. Burnout of connecting part in power plug 3. Contact local distributor.
or breaker.
B. Power is on, but the breaker has tripped 1. Electrical leakage 1. Contact local distributor.
immediately. 1-1. Insulation deterioration of heating
element.
1-2. Water leakage at attaching portion of
heating element.
1-3. Water seeping into breaker.
2. Short circuit 2. Contact local distributor.
2-1. Short circuit of heating element.
2-2. Short circuit of others.
C. After pressing START KEY, chamber 1. Breakage of heating element. 1. Contact local distributor.
temperature does not rise. 2. Burnout of wire connected to heating 2. Contact local distributor.
element
D. When pressing START KEY, error sound 1. Previous operation does not finish 1. Press STOP KEY to reset previous
honks and operation does not start. completely. operation. Press START KEY again to
start operation.
E. The temperature inside the chamber 1. Wrong electricity supply. 1. Check the required voltage of autoclave,
rises, but it does not rise to setting and then supply proper electricity to the
temperature. autoclave.
F. The temperature inside the chamber 1. Too much volume of objects in chamber. 1. Quantity of objects to be put inside of
rises to the set temperature, but chamber shall be less than 60% of capacity
sterilization has not been completed of the chamber. And each objects must
due to inside the chamber does not keep enough space between them.
become filled with saturated steam. 2. Shortage of sterilization time. 2. Increase sterilization time appropriately
in case of sterilization of liquid objects
and objects being large quantity.
3. Residual air inside container prevents 3-1. In case of objects being put in a
penetration of steam. sterilization bag, quantity of objects to
be put inside sterilization bag shall be
less than 60% of capacity of the
chamber. Sterilization bag must have
open mouth.
3-2. Containers shall not be superimposed
each other as possible as you can.
And set open mouth downward.
35
TROUBLE CONDITION CAUSES COUNTERMEASURES
G. Evacuating residual air does not stop 1. Breakage of exhaust valve. 1. Contact local distributor.
around 100℃. And the temperature 2. Over 1000m above sea level 2. This autoclave can not be used over
does not rise. 1000m above sea level.
H. Abnormal noise 1. Dusts are attached to lid gasket. 1. Stop operation and take away dusts.
2. Lid is out of alignment forcibly. 2. Contact local distributor.
I. Steam leakage from lid. 1. Deterioration of lid gasket. 1. Contact local distributor.
(cracks in the gasket or hardening)
2. Lid and chamber are out of alignment 2. Contact local distributor.
caused by lid opened too much or moving
main body holding only lid.
J. The lid lock lever can not be released 1. Power switch(breaker) is OFF. 1. Turn on power switch(breaker).
2. In operation or chamber temperature is 2. Press STOP KEY on the condition that the
over 80℃ even as after operation. TEMPERATURE DISPLAY indicates below
80℃ after completion of operation.
3. COMPLETE LED lights up after operation. 3. Press STOP KEY.
K. Movement of the lid lock lever is hard. 1. The lid lock lever is slid by force when lid is 1. Slide the lid lock lever slowly to close
not closed completely. position (left end) with pressing front part
of the lid cover.
(Especially TEMPERATURE DISPLAY
indicates over 60℃.)
2. Insufficient space between lid and clamps. 2. Contact local distributor.
L. No drainage 1. Drain pipe is choked. 1. Contact local distributor.
2. Stainless drain hose stands. 2. Lay stainless drain hose.
M. Drainage does not stop. 1. Breakage of drain valve. 1. Contact local distributor.
(Piece of glass or other objects came into
valve and damaged “Teflon ball” of valve.)
N. Leakage at attaching portion of 1. Screw loose. 1. Screw it up tight.
stainless drain hose. 2. Cracked joint portion between flexible 2. Contact local distributor.
portion and L-shaped portion.
O. Safety valve activates. 1. Residual air is not evacuated enough and 1-1. Quantity of objects to be put inside of
pressure in chamber rises abnormally due chamber shall be less than 60% of
to pressure of residual air. capacity of the chamber. And each
objects must keep enough space
between them.
1-2. In case of objects being put in a
sterilization bag , quantity of objects to
be put inside sterilization bag shall be
open mouth.
1-4. End of stainless drain hose must not be
choked up.
2. Due to the malfunction of temperature 2. Contact local distributor.
sensor, chamber pressure surpassed set
pressure of safety valve.
P. Pressure gauge does not indicate correct 1. Deterioration of pressure gauge. 1. Contact local distributor.
pressure value.
(Pressure gauge does not indicate 0MPa
when the lid is opened.)
Q. Er-0 is shown on the display. 1. Memory error of control unit. 1-1. Power off, then power on again.
1-2. Contact local distributor.
36
R. Er-1 is shown on the display with alarm 1. Autoclave works without water inside of 1. Turn off power switch(breaker) and let
and operation stops. chamber. stand for an hour. Because the chamber is
high temperature. And then pour water
into chamber.
2. Drain valve is opened. 2. Close drain valve.
3. Breakage of water lack preventive 3. Contact local distributor.
device.
4. Safety pressure switch activated. 4-1. In case of objects being put in a
(Residual air in the capacious container or sterilization bag, quantity of objects to
sterilization bag swelled.) be put inside sterilization bag shall be
open mouth.
S. Er-3 is shown on the display with 1. In a sterilization process, temperature 1-1. The distance between sensor and
alarm. inside chamber rises 2℃ higher than objects shall be kept more than 10mm.
setting temperature in certain period 1-2. Oleaginous objects must not be
of time. sterilized. (Oil caused unexpected rise in
This function is reset after dropping in temperature.)
temperature within range of ±2℃ from 1-3. Contact local distributor.
setting temperature.
T. Er-4 is shown on the display with alarm 1. Lid lock lever is not slid to close position 1. Slide the lid lock lever slowly to close
when starting operation. (left end) completely. position (left end) with pressing front part
of the lid cover.
2. Lid lock lever is slid by force when the lid 2. Slide the lid lock lever slowly to close
is not closed completely. position (left end) with pressing front part
of the lid cover.
3. Lock position jolts out of alignment by 3. If problem still exists after taking above
doing same operation as above several countermeasures, contact local distributor.
times.
U. Er-6 is shown on the display. 1. Battery for clock shutoff 1. Turn off power switch(breaker) and turn
on it again. Set the date and time.
2. IC in the control board is broken. 2. If problem still exists after taking above
countermeasure, contact local
distributor.
V. Er-7 is shown on the display. 1. Abnormal water supply - Sensor does not 1. Open faucet.
(only for automatic water supply function) detect water level for 3 minutes
due to the trouble of water supply.
(The city water faucet is closed)
W. Er-8 is shown on the display with 1. The printer (option) breakdown. 1. Contact local distributor.
alarm. (only for printer option)
X. Er-8 and Er-9 is shown on the display. 1. Running out of paper for the printer. 1. Set the new paper.
(only for printer option) 2. Printer cover is opened. 2. Close printer cover.
Y. Er-21or Er-22 is shown on the display 1. Breakage of control sensor or object 1. Contact local distributor.
with alarm. temperature sensor.
a. Inadequate drying. 1. Drying time is not sufficient. 1. Set drying time longer.
F 2. Drying temperature is low. 2. Set drying temperature appropriately not
O to have an influence on the objects
according to instruction manual.
R
3. Putting wet cloths into the chamber too 3. Avoid drying cloths as much as possible.
D
R
Y
I much. In case of drying cloths, do not fold nor
N
G overlap them.
M b. Automatic drainage does not 1. The strainer for drainage and/or drain pipe 1. Contact local distributor.
O activate. are choked up.
2. Automatic drain valve is broken. 2. Contact local distributor.
D
E
L c. Blower does not work in drying 1. Bacteria elimination filter is choked up. 1. Contact local distributor.
process. 2. Objects prevent the ventilation of air 2. Set the objects not to prevent the
hole. ventilation of air hole in the chamber.
37
７． Maintenance
●Regular Maintenance
Daily maintenance ① Replacement of water in the chamber

② Checking of the pressure gauge
Weekly maintenance ③ Cleaning of the chamber and exterior
④ Maintenance of lid gasket
Yearly maintenance ⑤ Periodic inspection
７．１ Daily maintenance
① Replacement of water in the chamber

● Way of water replacement
１． Check if pressure gauge indicates “0MPa”.
２． Open lid, and empty chamber.
(also base plate, use base plate hook for CL-40L)
３． Turn off power switch(breaker).
４． Lay the lever of drain valve and drain water in chamber.
５． After draining whole water in chamber, stand the lever to close drain valve.
６． If there is foreign object, remove it and load base plate.
７． Pour water to the same level as base plate.
Drainage method
Lay stainless drain hose, then lay the lever

of drain valve. If there isn’t heat-resistance
drain facility, use shallow container.
WARNING
When replacing water in chamber, check the chamber temperature cold enough.
Or it may cause burn injury.
38
CAUTION
Empty chamber, drain whole water in chamber, and close lid when autoclave is not used for over
a week.
Be sure to check water level and water quality in chamber. In case of lack, pour water up to
proper level. In case of impurity, refresh water in chamber.
Use base plate hook when unloading base plate.(CL-40L only)
② Checking of the pressure gauge
● Checking method
Check the indicator of pressure gauge points 0MPa when the lid is opened.
If 0MPa is not indicated, do not use. Contact local distributor.
WARNING
Do not use autoclave if pressure gauge does not indicate 0MPa when the lid is opened.
If pressure gauge does not work properly, it may cause serious accident and/or breakage of autoclave.
７．２ Weekly maintenance
③ Cleaning of the chamber inside and exterior
● Cleaning method
２． Open lid, and empty chamber.
(also base plate, use base plate hook for CL-40L)
４． Lay the lever of drain valve and drain water in chamber.
５． Remove dusts and foreign object, then wash wall and bottom of the chamber by
water.
６． Wipe off dirt with a soft cloth containing ethanol for cleaning the exterior.
(Do not use solvent such as thinner)
WARNING
Do not scratch heater in bottom of chamber when cleaning.
The water which infiltrates into heater may cause breakdown.
When cleaning of the chamber inside, check the chamber temperature cold enough.
Or it may cause burn injury.
39
CAUTION
Use base plate hook when unloading base plate.(CL-40L only)
④ Maintenance of lid gasket
● Cleaning method
２． Open lid.
４． Wipe off lid gasket with a soft cloth containing ethanol.
５． Wipe off the chamber edge with a soft cloth containing ethanol.
WARNING
CAUTION
Do not forcibly draw out, and do not transform the lid gasket.
It may cause burn and breakdown because of steam leak.
Clean the chamber edge with a soft cloth at the same time.
Or it may cause burn and breakdown because of steam leakage when dirt is on the edge.
７．３ Yearly maintenance
⑤ Periodic inspection
Execute following easy inspection. Check if;
1. the chamber and the lid are not damaged.

2. there is no leakage of the safety valve.
3. pressure gauge works properly.
4. the lid gasket does not harden nor have any cracks.
5. the lid and the chamber are not out of alignment.
6. there are not leakage nor loose of pipes and valves.
7. autoclave works properly.
40
７．４ Spare parts and replacement
Refer to the following list and replacement manual of main parts.
7.4.1 Spare parts list for model CL series
7.4.2 Replacement of the lid gasket
7.4.3 Replacement of pipe heater
If you intend to order these spare parts, please contact local distributor.
7.4.1 Spare parts list for model CL series

Model No. Model No.
No. Part name Photo No. Part name Photo
or spec. or spec.
Main board NCM Main switch AC100V～
1 225008 12
AC240V
Controller for CL series -10 Earth leakage breaker
SSR AQA411VL Heater relay

2 13 G7L-2A-B
Solid state relay for steam heater
DC24V 25A
Main circuit relay
Exhaust valve AC220V～ Check valve CVS-1/4

3 14
AC240V 80mmAq
Solenoid valve with fitting
Lid gasket CL-32 Pressure switch

4 15 80735
Molded silicon rubber CL-40
Steam heater Pressure safety valve

5 16
Sheathed pipe heater
Control sensor Rubber pan CL-32

6 YP-210SP 17
Resistance Pt100Ω, Class B Molded silicon rubber CL-40
Water lack preventer

7 55.13269.010
Thermostat
Pressure gauge
8 GK25-A18
0.7MPa CL-DP series
Drain valve unit Cartridge filter CCS-020

9 1
-C1H
Ball valve 0.2µm
Operation board NCM Air pump

10 225008 2 APN110-KEX
-20 Drying pump
Locking device Drain valve AC220V～

11 D4NL-1AFA-B 3
DC solenoid with safety switch
AC240V
Solenoid valve with strainer
Please specify the model name of autoclave and serial number when you order.
41
7.4.2 Replacement of the lid gasket
42
7.4.3 Replacement of pipe heater
E C Chamber
Gasket
Insulator Washer
3 Assembly nut
Wiring
1.Dismantlement
1) Pull off the 3pcs. of metal fittings"E" with pliers.
2) Open the both sides of the body and fall down sideways for easy work.
3) Disconnect wiring of the heater.
4) Open assembly nuts of the heater with spanners.(Opposite side 21)
5) Dismount the heater from bottom of the chamber.
6) Take off the sensor"C" from the heater.
2.Assembling
1) Install the new heater on bottom of the chamber.
Meantime, be sure to mount 2pcs. of the gaskets as above drawing.
2) Connect the wiring.
3.Checking
1) Fill water up to the base plate in the chamber and perform the allowable
maximum temperature.
Hereby, check current of the heater.
2) When the chamber temperature is reached , check steam leakage of the
mounted positions.
3) Check dimension and resistance of the Insulator.
43
８．Specifications
Model CL-32S CL-32L CL-40S CL-40M CL-40L
Heating method Electrical heater in chamber
Maximum operating pressure/temperature 0.27MPa/140℃ 0.25MPa/137℃
Operating Sterilization 40～140℃ 40～137℃
temperature range Dissolving/Warming Dissolving：40～99℃　Warming：40～60℃
Sterilization/Dissolving Sterilization/Dissolving：0～10hours00minutes, and continuous
Timer
Warming 0～48hours00minutes
AC220/230/240V, 1φ, 50/60Hz
Power source
12A 18A 20A
Chamber dimension　I.D.(mm)×D(mm) φ320×420 φ320×676 φ400×478 φ400×672 φ400×832
Capacity 34ℓ 54ℓ 60ℓ 85ℓ 105ℓ
Body dimension　W(mm)×D(mm)×H(mm） 550×550×790 550×550×950 620×650×790 620×650×980 620×650×1140
Weight 56kg 65kg 72kg 78kg 84kg
Chamber/Top plate/Base plate/Lid SUS304
44
Material Body Steel with melamine resin

Gasket Silicon rubber
Microprocessor controller, graphic display
Temperature：3digits, PID control
Controller
Timer：2digits hours / 2digits minutes, down count
（Temperature/Time integration system）
Double interlock system, Water lack preventive device,
Safety device Over temperature detector, Over pressure detector, Sensor break indicator,
Memory of power failure, Abnormal time indicator, Safety valve, Breaker
Hydrostatic test Conformity to the regulation of each country
Safety valve 0.29MPa 0.27MPa
Standard Stainless wire basket φ30×20cm×2pcs. φ30×30cm×2pcs. φ38×20cm×2pcs. φ38×30cm×2pcs. φ38×40cm×2pcs.
accessories Others Base plate, Stainless drain hose, Exhaust bottle(4ℓ) Left column+Base plate hook
POWER ON
PATTERN TEMPERATURE/ EXHAUST RESERVATION DATE/TIME OBJECT TEMP. PRE-HEATING SYSTEM

Setting flow chart
SETTING TIME SETTING SETTING OPERATION SETTING OPERATION OPERATION PARAMETER
STERILIZATION CONTROL TEMP. PRE-HEATING

STERILIZATION EXHAUST OFF MONTH YEAR
/DISSOLVING DIFFERENCE ON
TEMPERATURE
OBJECT AUTOMATIC
OBJECT TEMP. AUTOMATIC
TEMPERATURE WATER
SENSOR
SENSOR WATER SUPPLY
STERILIZATION/ STERILIZATION SUPPLY
EXHAUST ON DAY MONTH
WARMING /DISSOLVING
TIME
45
DISSOLVING/ WARMING/ EXHAUST
HOUR DAY
WARMING DRYING TEMP. TEMPERATURE
STERILIZATION/ WARMING/
MINUTE HOUR
DRYING DRYING TIME
DRYING MINUTE
ＣＬ-ＤＰ
AC100V
ELB
L
DB X1a2 X1a1
Block diagram
CN4 CN10 1 2
8 30P
7
6
5
MLD B'
4
3 B
2 A
B' H1
1 S1
B + -
CN13 MB A
X1 2
D TB1
1 SSR1
PS EGO CN12
4 ①
3
2
1
CN17
46
2 -
10P
1 +
CN8
2 - CN11
1 + 1
CN6 2
2 - CN7
1 +
CN3 1 1
7 2 2
LS1
6 3 3
5 CN15 CN14
LS2
4 1
3 2
2 3
1 4
DC24V 5
++-- CN1
CN2 4 3 2 1 CN5 3 2 1 PE
0.1A
EV1
DC24V AC100VAC240V
N L
SP1
1 2 3 4
--++
5 4 3 2 1
FG
PE
Memo
47
Responsibility
Please follow the instructions in this manual when using this equipment.
We have no responsibility for the accidents or breakdown of device if it is used with a failure to comply.
Never conduct what this manual forbids.

Unexpected accidents or breakdown may result in.
Note
● The contents of this manual may be changed without notice.
● This manual with missing pages may be replaced.
Instruction Manual for

AUTOCLAVE Laboratory use
CL-32S/32L/40S/40M/40L
Second Edition Feb. 21, 2013
ALP Co., Ltd.

3-3-10, Midorigaoka, Hamura-shi,
Tokyo, Japan
Zip code : 205-0003
TEL: +81 (0)42-579-0531
FAX: +81 (0)42-579-0533
http：//www.alpco.co.jp
E-mail: alpco@wonder.ocn.ne.jp
48

CL-32S/32L 40S/40M/40L: Instruction Manual

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QD-200-P126-R2

● To use this product safely, make sure to read this manual.

2. For the safety use P.4～7

3. Part names P.8～9

5. Operation method P.15～34

6. Trouble shooting P.35～37

● Setting flow chart P.45

● Thank you for choosing ALP products.

● Please do understand and observe safety instructions written in this manual.

Base plate, 1pc.

Stainless wire basket

Base plate hook, 1pc.

The meaning of each illustration is as follows;

In case of power failure during operation, autoclave works as follows.

1. Power to autoclave is lost, all controls stop.

Stainless drain hose

1. Pressure gauge 15. EXHAUST LED

This autoclave has casters. So it can move horizontally.

４．２ Installation location

Wall surface The end of stainless drain hose

Lock front casters

Level and hard floor

４．３ Installation condition

Autoclave must be installed under following condition.

Do not install autoclave locations as follows

４．４ Power cable connection

４．５ Protective grounding

●Base plate・・・Put it on the bottom of chamber.

●Stainless drain hose・・・Set as figure 1.

Hitch ring to hook when needed

Connect the end of stainless drain hose

Figure 1 How to set stainless drain hose

Figure 2 Case of using drainage channel

●Stainless wire basket・・・ Use it during operation.

●Base plate hook(CL-40L only)・・・Keep it with this instruction manual.

● Flow chart of basic operation

１. Turn on power switch(breaker)

２. Close drain valve

５. Close and lock the lid

６. Press STOP KEY

７. Set operation pattern,

８. Press START KEY

１０. Unload object

１１. Close and lock the lid

１２. Turn off power switch(breaker)

５．１ Turn on power switch(breaker)

① Check if power cable is connected properly.

５．２ Close drain valve

① Stand the lever of drain valve to close drain valve.

① Check if pressure gauge indicates “0MPa”.

Base plate Chamber

※Do not put too much objects in chamber.

Open mouth during

●How to use the stainless bucket

③． Put water (200ml) into stainless bucket.

⑤． Do not put too much objects in chamber for sterilization.

⑥． Sterilization time / temperature shall be longer/higher, as evacuating air's efficiency of

① Grip lid hold and close lid softly.

① Press STOP KEY to reset previous operation process.

changed with CHANGE▲▼　KEY.

changed with CHANGE▲▼　KEY.

５．１１ Close and lock the lid