7.75025.0005-xxxxxxxxxx msp.

February 2020

1.00822.0250 6. Preparation
• The pH must be within the range 1 - 10.
Adjust, if necessary, with sodium hydroxide solution or hydrochloric
Spectroquant ® acid.

Fluoride Test F- • Filter turbid samples.

7. Procedure
Measurement Blank
1. Method sample (only 1x
Fluoride ions react with part of a red zirconium-dye solution to form a per series)
colorless complex, which in turn results in a bleaching of the red color Pretreated sample 5.0 ml - Pipette into a test tube.
(SPADNS method). The concentration of the unconsumed zirconium-dye (15 - 30 °C)
solution is subsequently determined photometrically.
Distilled water
1)
- 5.0 ml Pipette into a test tube.
The method is analogous to APHA 4500-F- D. (15 - 30 °C)
Reagent F-1 1.0 ml 1.0 ml Add with pipette and
2. Measuring range and number of determinations mix.

Cell Measuring range Reagent Leave to stand for 1 min (reaction time), then fill the measurement
sample and the blank into two separate 50-mm semi-microcells and
50 mm 0.02 - 2.00 mg/l F
-
250 ml measure in the photometer.

For programming data for selected photometers / spectrophotometers see It is recommended to use water for analysis EMSURE®, Cat. No. 116754.
1)

sigmaaldrich.com/photometry. For measurement in the 50-mm cell the sample volume and the volume of the
reagent F-1 must be doubled.

3. Applications
Notes on the measurement:
This test does not measure any complex fluorine compounds as occur in
some cases in galvanic waste water. • For photometric measurement the cells must be clean.
Wipe, if necessary, with a clean dry cloth.
Sample material:
Groundwater and surface water • Measurement of turbid solutions yields false-high readings.
Sea water after appropriate sample pretreatment (distillation analogous to • The pH of the measurement solution must be below 1.
APHA 4500-F- B) • The color of the measurement solution remains stable for 60 min after
Drinking water the end of the reaction time stated above.
Mineral water (optionally after appropriate sample pretreatment: distilla-
tion analogous to APHA 4500-F- B)
Wastewater after appropriate sample pretreatment (distillation analogous
8. Analytical quality assurance
to APHA 4500-F- B) recommended before each measurement series
Percolating water To check the photometric measurement system (test reagent, measure-
ment device, handling) and the mode of working, the fluoride standard
solutions CRM (see section 5) can be used.
4. Influence of foreign substances Sample-dependent interferences (matrix effects) can be deter-
This was checked individually in solutions containing 1 and 0 mg/l F-. The mined by means of standard addition.
determination is not yet interfered with up to the concentrations of foreign Additional notes see under www.qa-test-kits.com.
substances given in the table. Cumulative effects were not checked; such For quality and batch certificates for Spectroquant® test kits see the
effects can, however, not be excluded. website, where you will find all data in production control, that are
determined in accordance with ISO 8466-1 and DIN 38402 A51.
Concentrations of foreign substances in mg/l, %, or °e

Al3+ 250 EDTA 1 9. Notes

Ca2+ 500 Free chlorine 5
CO3 500
2-
Anionic surfactants 1)
10 • Reclose the reagent bottle immediately after use.
Cu2+ 25 Cationic surfactants 2) 10 • Information on disposal can be obtained at
Fe3+ 5 Nonionic surfactants 3) 750 www.disposal-test-kits.com.
Mg2+ 100 Total hardness 87.5 °e
NH4+ 250 Na-acetate 0.2 %
Ni2+ 1000 NaCl 0.2 %
NO2- 1 NaNO3 0.2 %
PO43- 1 Na2SO4 0.002 %
SiO32- 100
Zn2+ 1000


1)
tested with Na-dodecyl sulfate
2)
tested with N-cetyl-N,N,N-trimethylammonium bromide
3)
tested with Triton® X-100

5. Reagents and auxiliaries

Please note the warnings on the packaging materials!
The test reagent is stable up to the date stated on the pack when stored
closed at +15 to +25 °C.

Package contents:
1 bottle of reagent F-1
1 AutoSelector
Other reagents and accessories:
MQuant® Universal indicator strips pH 0 - 14, Cat. No. 109535
Sodium hydroxide solution 1 mol/l Titripur ®, Cat. No. 109137
Hydrochloric acid 1 mol/l Titripur ®, Cat. No. 109057
Water for analysis EMSURE®, Cat. No. 116754
Fluoride standard solution CRM, 0.200 mg/l F, Cat. No. 132234
Fluoride standard solution CRM, 0.50 mg/l F, Cat. No. 132233
Fluoride standard solution CRM, 1.00 mg/l F, Cat. No. 132235
Fluoride standard solution CRM, 1.50 mg/l F, Cat. No. 132236
Pipettes for pipetting volumes of 1.0, and 5.0 ml
Semi-microcells 50 mm (2 pcs), Cat. No. 173502
Rectangular cells 50 mm (2 pcs), Cat. No. 114944

Merck KGaA, 64271 Darmstadt, Germany,

Tel. +49(0)6151 72-2440
www.sigmaaldrich.com/photometry
EMD Millipore Corporation, 400 Summit Drive
Burlington MA 01803, USA, Tel. +1-978-715-4321
Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd.
2149 Winston Park, Dr. Oakville, Ontario, L6H 6J8
Phone: +1 800-565-1400