1.14878.0001 7. Preparation 1.14879.0001 • Analyze immediately after sampling. • The pH must be within the range 2 - 12 Adjust, if necessary, with sulfuric acid. Spectroquant® TOC Cell Test TOC 8. Procedure 114878 114879 Pretreated sample 25 ml 1.0 ml Place into a suitable glass (10 - 30 °C) vessel. 1. Definition Distilled water 1) - 9.0 ml Add and mix. The term TOC (Total Organic Carbon) is used to describe the total content of Reagent TOC-1K 3 drops 2) 2 drops 2) Add and mix. organically bound carbon in dissolved and undissolved compounds. The pH must be below 2.5. Check with pH-indicator The TOC content is expressed in mg/l. strips. Adjust the pH, if necessary, 2. Method with sulfuric acid.
By digestion with sulfuric acid and peroxodisulfate, carbon-containing com- Stir for 10 min at medium speed. pounds are transformed into carbon dioxide. This reacts with an indicator solu- tion, the color of which is determined photometrically. Stirred sample 3.0 ml 3.0 ml Pipette into a reaction cell. Inorganically bound carbon (dissolved carbon dioxide and anions of carbon dioxide) is expelled in gaseous form beforehand by acidification. Reagent TOC-2K 1 level grey 1 level grey Add. The digestion is analogous to APHA 5310 C (2014). microspoon microspoon (in the cap of the (in the cap of the TOC-2K bottle) TOC-2K bottle) 3. Measuring range and number of determinations Immediately close the cell tightly with an aluminium cap. Heat the cell, standing on its head, at 120 °C in the preheated thermoreactor for 120 Measuring range Number of Cat. No. + 5 min. mg/l TOC determinations Leave the closed cell, standig on its head, to cool in a test-tube rack for 60 min (cooling 114878 5.0 - 80.0 time). 25 Do not cool with cold water! 114879 50 - 800 After cooling, turn the cell upright and measure in the photometer within 10 min. For programming data for selected photometers / spectrophotometers see It is recommended to use water for chromatography LiChrosolv®, Cat. No. 115333. 1)
www.service-test-kits.com. Hold the bottle vertically while adding the reagent!
2)
4. Applications Notes on the measurement:
This test measures the total content of organically bound carbon. • The measurement accuracy can be increased by measuring against a separately prepared blank (preparation as per measurement sample, but with Sample material: distilled water instead of sample). Groundwater and surface water Drinking water • When the sample is stirred for 60 min, the determination is still not interfered with even in the case of TIC contents of up to 100 mg/l (Cat. Production control Wastewater No. 114878) or, respectively, up to 1000 mg/l (Cat. No. 114879). This test is not suited for seawater. • For photometric measurement the cells must be clean. Wipe, if necessary, with a clean dry cloth. 5. Influence of foreign substances • Measurement of turbid solutions yields false-low readings. This was checked individually in solutions containing 50 (Cat. No. 114878) or • After the measurement remove the aluminium cap from the cell and replace by the screw cap. After cleaning with distilled water and drying, the 500 (Cat. No. 114879) and 0 mg/l TOC. The determination is not yet interfered aluminium cap can be used for further determinations. with up to the concentrations of foreign substances given in the table. Cumulative effects were not checked; such effects can, however, not be excluded. 9. Analytical quality assurance Concentrations of foreign substances in mg/l or % recommended before each measurement series 114878 114879 114878 114879 To check the photometric measurement system (test reagents, measurement device, handling) and the mode of working, the TOC standard solutions Ca2+ 200 1000 TIC 1) 25 250 Mg2+ 200 1000 NaCl 0.5 % 5% CRM (see section 6) can be used. These standard solutions must be analyzed NH4-N 100 1000 NaNO3 10 % 20 % as described in section 8. Na2SO4 10 % 20 % Sample-dependent interferences (matrix effects) can be determined by means of standard addition. 1) Total Inorganic Carbon; the tolerable values can be raised by a factor of four when the Additional notes see under www.qa-test-kits.com. sample is stirred for 60 min (see section 8). For quality and batch certificates for Spectroquant® test kits see the website, where you will find all data in production control, that are determined in 6. Reagents and auxiliaries accordance with ISO 8466-1 and DIN 38402 A51.
Please note the warnings on the packaging materials!
10. Notes The test reagents are stable up to the date stated on the pack when stored closed at +15 to +25 °C. • Reclose the reagent bottles immediately after use. • Information on disposal can be obtained at www.disposal-test-kits.com. Package contents: 1 bottle of reagent TOC-1K 1 bottle of reagent TOC-2K 25 reaction cells 1 sheet of round stickers for numbering the cells Also required: Aluminium caps (“Screw caps for TOC digestion”, 6 pcs), Cat. No. 173500 Other reagents and accessories: MColorpHastTM Universal indicator strips pH 0 - 14, Cat. No. 109535 Sulfuric acid 0.5 mol/l Titripur®, Cat. No. 109072 MColorpHastTM pH-indicator strips pH 0 - 6.0, Cat. No. 109531 Water for chromatography LiChrosolv®, Cat. No. 115333 TOC standard solution CRM, 10.0 mg/l TOC, Cat. No. 132247 (for Cat. No. 114878) TOC standard solution CRM, 25.0 mg/l TOC, Cat. No. 132248 (for Cat. No. 114878) TOC standard solution CRM, 50.0 mg/l TOC, Cat. No. 132249 (for Cat. No. 114878) TOC standard solution CRM, 100 mg/l TOC, Cat. No. 132251 (for Cat. No. 114879) TOC standard solution CRM, 200 mg/l TOC, Cat. No. 132252 (for Cat. No. 114879) TOC standard solution CRM, 500 mg/l TOC, Cat. No. 132253 (for Cat. No. 114879) Pipettes for pipetting volumes of 1.0, 3.0, and 9.0 ml Thermoreactor
Merck KGaA, 64271 Darmstadt, Germany,
Tel. +49(0)6151 72-2440 www.analytical-test-kits.com EMD Millipore Corporation, 290 Concord Road, Billerica, MA 01821, USA, Tel. +1-978-715-4321
