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    Potato Dextrose Agar EP/USP/BAM: Industry Regulations

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    Dylan Unrau
    Potato Dextrose Agar is a general purpose medium for culturing yeasts and molds. It contains potato starch as a nutrient source, dextrose as a carbohydrate, and agar as a solidifying agent. The medium encourages rich growth of fungi and molds and can be used to enumerate and identify yeasts and molds through incubation and examination of colony morphology. Potato Dextrose Agar is recommended by regulatory bodies like the USP and EP for testing of non-sterile products and cultivation of fungi.

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    Potato Dextrose Agar EP/USP/BAM: Industry Regulations

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    Dylan Unrau
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    Potato Dextrose Agar is a general purpose medium for culturing yeasts and molds. It contains potato starch as a nutrient source, dextrose as a carbohydrate, and agar as a solidifying agent. The medium encourages rich growth of fungi and molds and can be used to enumerate and identify yeasts and molds through incubation and examination of colony morphology. Potato Dextrose Agar is recommended by regulatory bodies like the USP and EP for testing of non-sterile products and cultivation of fungi.

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    Potato Dextrose Agar is a general purpose medium for culturing yeasts and molds. It contains potato starch as a nutrient source, dextrose as a carbohydrate, and agar as a solidifying agent. The medium encourages rich growth of fungi and molds and can be used to enumerate and identify yeasts and molds through incubation and examination of colony morphology. Potato Dextrose Agar is recommended by regulatory bodies like the USP and EP for testing of non-sterile products and cultivation of fungi.

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    21 views2 pages

    Potato Dextrose Agar EP/USP/BAM: Industry Regulations

    Uploaded by

    Dylan Unrau
    Potato Dextrose Agar is a general purpose medium for culturing yeasts and molds. It contains potato starch as a nutrient source, dextrose as a carbohydrate, and agar as a solidifying agent. The medium encourages rich growth of fungi and molds and can be used to enumerate and identify yeasts and molds through incubation and examination of colony morphology. Potato Dextrose Agar is recommended by regulatory bodies like the USP and EP for testing of non-sterile products and cultivation of fungi.

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    Potato Dextrose Agar EP/USP/BAM Cat.

    1022
    For the identification, cultivation and enumeration of yeast and molds.

    Practical information
    Aplications Categories
    Selective enumeration Yeasts and molds

    Industry: Pharmaceutical/Veterinary / Clinical / Food

    Regulations: USP / European Pharmacopoeia / BAM

    Principles and uses


    Potato Dextrose Agar is recommended by APHA and FDA for culturing yeast and molds. It can also be used in the identification of fungi and yeasts in
    parallel with their cellular morphology, or in methods of micro cultivation in slides.

    This general purpose medium can be supplemented with acid or antibiotics to inhibit bacterial growth. The nutritionally rich base (potato infusion)
    encourages a very rich fungal and mold growth. Dextrose is the fermentable carbohydrate as a carbon and energy source. Bacteriological Agar is the
    solidifying agent.

    The European Pharmacopoeia, USP recommends this medium in the paragraph 2.6.12: “Microbiological examination of non-sterile products: Microbial
    enumeration test” for the preparation of the test strain in the examination of TYMC.
     

    Formula in g/L
    Bacteriological agar 15 Dextrose 20
    Potato starch (equivalent to 200 g of Infusion from potatoes) 4
     

    Preparation
    Suspend 39 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete
    dissolution. Sterilize in autoclave at 118-121 ºC for 15 minutes. Cool to 45-50 ºC, mix well and dispense into plates.
     

    Instructions for use


    » For clinical diagnosis, the type of sample are all clinical samples (hair, skin, nails etc.).

    - Inoculate the surface streaking in parallel with the handle or swab. If the samples are formed by scrapes of skin, hair or nails, place the material in the
    center of the surface of the medium.
    - Incubate at 25-30 ºC or 20-25 ºC for 18-48 hours and up to 7 days depending on the examined microorganism.
    - Reading and interpretation of the results.

    » For other uses not covered by the CE marking:

    Cultivation and enumeration of yeast and molds:


    - Inoculate the Potato Dextrose Agar plates in order to obtain isolated colonies.
    - When the medium is to be used for the enumeration of yeasts and molds, the pH should be lowered to inhibit bacteriological growth. Add to the cooled
    to 45-50 ºC sterilized medium, approximately 14 ml of a sterilized 10% solution of tartaric acid to obtain a pH of 3,5. Do not reheat the adjusted medium
    after adding the acid because the agar may hydrolyze and not solidify.
    - Incubate plates at 25-30 ºC for 5-7 days forTrichophyton mentagrophytes, at 20-25 ºC for 5-7 days, or until good sporulation is achieved for Aspergillus

    Inspired by knowledge Page 1 of 2 - Revision number 2 - Date 04/10/2019 www.condalab.com


    brasiliensis and at 25-30 ºC for 18-48 h for Candida albicans and Saccharomyces cerevisiae.
    - Yeasts will grow as cream to white colonies. Molds will grow as fuzzy colonies of various colors.
    - To differentiate and isolate genus and species, carry out further microscopic and biochemical tests.
     

    Quality control
    Solubility Appareance Color of the dehydrated medium Color of the prepared medium Final pH (25ºC)
    Slightly opalescent Fine powder Beige Light amber 5,6±0,2
     

    Microbiological test
    Incubation conditions: Trichophyton mentagrophytes (25-30 ºC / 5-7 days); Aspergillus brasiliensis (20-25 ºC / 5-7 days or until good sporulation is
    achieved); Candida albicans and Saccharomyces cerevisiae (25-30 ºC / 18-48 h).

    Microrganisms Specification
    Candida albicans ATCC 10231 Good growth
    Aspergillus brasiliensis ATCC 16404 Good growth
    Trychophyton mentagrophytes ATCC 9533 Good growth
    Saccharomyces cerevisiae ATCC 9763 Good growth
     

    Storage
    Temp. Min.:2 ºC
    Temp. Max.:25 ºC
     

    Bibliography
    American Public Health Association. Standard Methods for the Examination of Dairy Products, 13th Ed. APHA, Inc. New York, 1960.
    American Public Health Association. Recommended Methods for the Microbiological Examination of Foods. APHA, New York, 1958.
    Association of Official Analytical Chemists. 1995. Bacteriological analytical manual, 8th ed. AOAC International. Gaithersburg, MD.
    European Pharmacopoeia 9.3

    Inspired by knowledge Page 2 of 2 - Revision number 2 - Date 04/10/2019 www.condalab.com

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