Stat Fax 2200: Microplate Incubator/Shaker Operator's Manual
Stat Fax 2200: Microplate Incubator/Shaker Operator's Manual
Stat Fax 2200: Microplate Incubator/Shaker Operator's Manual
Microplate Incubator/Shaker
Operator’s Manual
1. Introduction ...................................................................................................... 1
1.2 Safety Symbols Le Symboles de Sûreté ................................................................................. 1
1.3 Safety Terms Terminologie de Sûreté ..................................................................................... 1
1.4 Safety Precautions ................................................................................................................... 2
2. Installation ........................................................................................................ 3
5. Specifications................................................................................................... 5
8. Information ....................................................................................................... 9
1. Introduction
Stat Fax® 2200 is an economical combination incubator, timer, and shaker that accommodates two standard
microplates or strip trays. This microprocessor controlled instrument offers variable temperatures, times, and
mixing speeds via the keypad. Incubation, timing, and mixing may also be used independently. A pulse mode is
available. Temperatures may be set from room temperature to 40°C with a resolution of 0.1 degree. The
instrument is designed to provide long life and trouble-free performance. A smoke tint acrylic cover protects the
plates, shields them from light, and insulates them during incubation. The enclosed system also reduces the risk
of biohazard from the dispersal of aerosols. Like all of the Stat Fax® instruments, this instrument offers quality
and versatility and is backed with a one year warranty.
These terms may appear on the product: Les marques sur le produit:
These terms may appear in this manual: Les marques dans l’opérateur manuel:
Do Not Operate With Suspected WARNING: If you suspect there is damage to this instrument, have it inspected by
Failures a qualified service person.
Do Not Operate in Wet/Damp WARNING: Do not operate instrument in wet/damp conditions.
Conditions
Do Not Operate In An Explosive WARNING: Do not operate instrument in an explosive atmosphere.
Atmosphere
CAUTION! L’ATTENTION!
BIOHAZARD: If any materials are overturned during operation, immediately set
the power switch to OFF. This material should be treated as potentially
biohazardous. Appropriate cleanup and disposal of biohazardous waste should
be used.
Avertissement! Lors du fonctionnement, si on renverse des matériaux, coupez
immédiatement le courant. Placez le commutateur électrique a AU LOIN(0). Traitez le
matériel comme biohazardous, utilisant approprie nettoient et des méthodes de
disposition.
2. Installation
Carefully unpack the instrument and remove it from its plastic bag. Report any obvious damage to your freight
carrier at once.
Note: Retain the original packing materials for future use in the event that the instrument is shipped to another
location or returned for service.
Place the instrument on a flat working surface capable of safely supporting the weight of the instrument
(approximately 7 lbs). A clearance of at least 8 cm around the instrument is required to assure optimal ventilation.
3. Power Requirements
Either of the following power units may be used:
120VAC input, 12VAC, 2 Amp output
230VAC input, 12VAC, 2 Amp output
Use a power unit approved for the country of use. For use inside the US: Use only a NRTL listed plug-in Class 2
transformer with rated output 12VAC, 2A.
A power unit is supplied with the instrument. This type of power unit is commonly referred to as a wall
transformer, direct plug-in transformer, power adaptor, or simply an adaptor and utilizes an isolation transformer.
Plug the power unit into the instrument jack on the rear panel, and into the wall outlet. The ON/OFF switch is
located on the back of the instrument. When you turn the instrument on, you will see "rdY" (indicating READY) on
the instrument display.
Four flexible legs support the tray. A microprocessor controls the speed of an orbiting counterweight mechanism
that shakes the tray. The user may select any one of 8 speeds ranging from 575 rpm to 1500 rpm.
The following diagram describes the key functions for the instrument. More details on the operation of each
feature are provided in Section 8, Operating Procedures.
NOTE: Many keys have more than one function. The numbers apply only to setting timer, temperature, and
speed. Otherwise, the function which appears above the key defines the function of the key.
Mixer
Speeds: ............................................................................. 8 mix speeds, from 575 to 1500 rpm, timed duration on
continuous and mix pulse, 1500 rpm, for 3 second
duration.
Mechanism: ...................................................................... orbital mixing by rotating counterweight
Timer
Modes: .............................................................................. set time, view time remaining, audible signal, stop mixing
(maintains temperature control after time out)
Resolution: ........................................................................ one second
Range: .............................................................................. one second to 99 minutes 59 seconds
Electronic
Display: ............................................................................. four, 7-segment, red LEDs
Keypad: ............................................................................. 16-key, domed membrane switch, enunciating, 4x4
Power requirements:......................................................... 12V AC, 50-60Hz, 2 amp, power unit available for 120V
or 230V power sources. Use power unit approved for
country of use.
Microprocessor: ................................................................ Z80A
Certifications and Compliances: ....................................... NRTL Listed CE Certification
Other
Vessel: ............................................................................ holds 2 standard microtiter plates or strip trays
Enclosure: ....................................................................... fire retardant ABS plastic enclosure with metal base
Aerosol guard: ................................................................. smoke-tint acrylic cover
Dimensions: .................................................................... approx. 10 x 12½ x 6 inches (26 x 32 x 16 cm) with
cover, 7 lbs. (3 kg)
2. Lift off the smoked acrylic cover. Open the internal cover by pulling back on the metal clip while lifting the
front of the clear plastic lid. The covers serve as aerosol guards as well as insulators and should be used at all
times.
3. The instrument is designed to hold two standard 96-well microplates or strip trays, round or flat bottom wells.
To properly seat a microplate in the Incubator/Shaker, slide the top edge of the microplate into the silicon
bumpers located in the back two corners of the plate holder. By applying pressure to these bumpers with the
plate, the bottom edge of the plate should click into place. Press BOTH plates into the bumpers located at the
rear corners of the metal frames in the incubation platform. Close the internal cover and replace the outer cover.
6.1 Incubating
To set the incubator temperature, press the SET TEMP key. It will display the previously set temperature. If this
is the desired temperature, press ENTER; otherwise, type the desired temperature in Celsius, using the numeric
keys, with no more than one place after the decimal point. Press the ENTER key. The instrument will pause to
measure and then display the current temperature. (The displayed temperature is corrected to reflect the
equilibrated temperature inside a micro-well containing 200µL of water.) Then, either heating will begin or heating
will stop depending on the set temperature, until the desired temperature is reached. For faster cooling, open
both lids and press the “6” key to turn on the fan. The display will continue to show the incubator temperature,
unless another function is selected. A decimal point indicator under the degree symbol reflects the state of the
heater. When it is lit the heater is on. When first set, the instrument may over and undershoot the desired
temperature but will quickly adjust and hold the temperature constant. When desired temperature is achieved, the
heat will pulse on and off to maintain the temperature within +/- 0.5 degrees. The fan may run periodically during
heating to prevent water condensation on the internal lid.
To check the temperature when it is not being displayed, press the VIEW TEMP key. The temperature will then
be continuously displayed until another function is selected.
If no temperature is entered the unit automatically controls the temperature to 25°C or ambient, whichever is
greater.
To set the mixer, press the MIX key. The first time the mixer is run after being plugged in, it will display “0-7.”
Type the number of the desired mixing speed. Use the up and down arrows to increase or decrease the speed
respectively. Pressing the STOP key will stop the mixer. To view the current speed while mixing, press the MIX
key. The actual rpm’s of the speed settings 0-7 are as follows:
0 575
1 625
2 675
3 750
4 875
5 1000
6 1200
7 1500
The orbital diameter varies with speed, being about 2 mm at setting 0, 1.5 mm at setting 2, and 1 mm at settings
4-7.
6.3 Timing
To set the timer, press the SET TIME key. The display will show "0.00". Enter one or two digits for the minutes
and then a decimal point followed by two digits for the seconds, then press ENTER. If the time has been set since
the last power-up, the previously set time will be displayed. If this is the desired time, press ENTER. The
maximum time the timer can be set for is 99 minutes and 59 seconds. Press the ENTER key to begin timing.
The display will show a countdown of the time remaining.
When the time has elapsed, the mixing will stop and a beeper will sound. Press the CLEAR key to silence the
beeper. Temperature will continue to be maintained after the timing cycle ends.
To turn off the timer while it is running, press the SET TIME key and push CLEAR twice. The display will prompt
"OFF" and the timer will be disabled.
To check the time remaining when it is not being displayed, press the VIEW TIME key. The countdown will be
displayed.
6.4.3 Condensation
As the plates are heated, condensation may form on the inner lid. The instrument periodically cycles the fan
to clear this. In addition, you may press the “6” key to manually turn on the fan for 15 seconds.
7.1 Maintenance
7.1.1 Exterior
CAUTION: Solvents such as acetone or paint thinner will damage the instrument! Use only water and
recommended cleaners! Avoid abrasive cleaners. The display area is liquid-resistant, but is easily
scratched.
The exterior of the instrument may be cleaned with a soft cloth using plain water. Alternately, you may use
any of the standard disinfectants used for laboratory decontamination. A 10% solution of chlorine bleach
(5.25% Sodium Hypochlorite) or 70% isopropyl alcohol may be used as a disinfectant. Take special care
not to spill any liquid into the read well.
7.2 Accessories
Factory:
Telephone: USA 772-283-6540
Fax: USA 772-283-8020
E-mail: support@awaretech.com
Mailing Address:
Important: When contacting us, please have the Model and Serial Number of the instrument in question.
Have a description of the problem with as much detail as possible. Save any relevant printouts and send or
e-mail us the information.
Model:_________________
Serial #:________________