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    Mock Lab Chromatography

    Uploaded by

    Faruk Ibrahimovic
    This lab report summarizes an experiment to identify pigments in dandelion buds and stalks using chromatography. The student hypothesized that only chlorophyll a or b would be identified. Variables like plant type, solvent, and amount of solvent were considered. Materials included chromatography paper, solvent, and plant parts. The procedure involved extracting pigments using solvent and developing the chromatography paper. A single olive-green pigment was identified as pheophytin based on its Rf value and color. The student was satisfied with the results but noted some uncertainties.

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    Mock Lab Chromatography

    Uploaded by

    Faruk Ibrahimovic
    110 views5 pages
    This lab report summarizes an experiment to identify pigments in dandelion buds and stalks using chromatography. The student hypothesized that only chlorophyll a or b would be identified. Variables like plant type, solvent, and amount of solvent were considered. Materials included chromatography paper, solvent, and plant parts. The procedure involved extracting pigments using solvent and developing the chromatography paper. A single olive-green pigment was identified as pheophytin based on its Rf value and color. The student was satisfied with the results but noted some uncertainties.

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    This lab report summarizes an experiment to identify pigments in dandelion buds and stalks using chromatography. The student hypothesized that only chlorophyll a or b would be identified. Variables like plant type, solvent, and amount of solvent were considered. Materials included chromatography paper, solvent, and plant parts. The procedure involved extracting pigments using solvent and developing the chromatography paper. A single olive-green pigment was identified as pheophytin based on its Rf value and color. The student was satisfied with the results but noted some uncertainties.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    110 views5 pages

    Mock Lab Chromatography

    Uploaded by

    Faruk Ibrahimovic
    This lab report summarizes an experiment to identify pigments in dandelion buds and stalks using chromatography. The student hypothesized that only chlorophyll a or b would be identified. Variables like plant type, solvent, and amount of solvent were considered. Materials included chromatography paper, solvent, and plant parts. The procedure involved extracting pigments using solvent and developing the chromatography paper. A single olive-green pigment was identified as pheophytin based on its Rf value and color. The student was satisfied with the results but noted some uncertainties.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    You are on page 1of 5

    Teacher: Stanislava Marjanović Subject: Chemistry School: Druga Gimnazija Sarajevo

    Faruk Ibrahimović

    Lab report:

    Chromatography

    6.10.2021
    Aim: Extract and identify the pigments in the bud and stalk of a dandelion

    Research question: What pigments are contained in the bud and stalk of a dandelion?

    Background information:

    - Chromatography is a laboratory technique for the separation of a mixture. A solvent is

    a substance that has can dissolve a mixture. Solubility is the property of a substance to

    be dissolved in a solvent. A chromatography paper is a kind of paper used for

    chromatography. Pigment is any group of compounds that is intensely coloured.

    Hypothesis:

    o I think that the only pigment I will be only to identify will be chlorophyll a or b since

    the bud isn’t fully grown, and chlorophylls are the most common pigments found in

    green plants.

    Variables:

    o Independent variables

    o Plant used – this variable can completely change the result as different

    types if plants have different types of pigments

    o Dependent variables

    o Retention values - this variable is affected by the solvent used on the plant,

    since not every plant can be dissolved by every solvent and if the plant is not

    properly dissolved, then the pigments won’t come out properly leading to bad

    retention values

    o Controlled variables

    o Amount of solvent used – This variable can affect the retention factor

    pg. 2
    o Solvent – this variable directly affects what pigment in it will be extracted as

    not every solvent can dissolve every plant

    Materials:

    o Chromatography paper

    o Mortar and pestle

    o Beaker

    o 50ml of methanol

    o Bud and stalk of dandelion

    Procedure:

    1. Crush the plant up with a small amount of solvent for about 5 minutes

    2. Take everything that is still solid out

    3. Draw a line 3cm from the bottom of the chromatography paper

    4. Pour 5 drops of the mixture on the line

    5. Fix the paper in the beaker

    6. Pour the rest of the solvent in the beaker making sure it does not go over the line

    7. Wait for 3 hours

    8. Take the chromatography paper out and let it dry

    9. Measure and note the travel distance of the solvent and pigment

    pg. 3
    Raw data:

    Image 1: the chromatography paper after the procedure

    Path travelled by the solvent(L1): 15.2cm

    Path travelled by pigment(L2): 12.5cm

    Colour of pigment: olive-green

    pg. 4
    Processed data:

    Rf value: L2 / L1 = 15.2cm / 12.5 cm = 0.8

    This value and colour correspond to the pigment pheophytin1.

    Uncertainties:

    - Ruler – 0.1 cm

    Evaluation and conclusion:

    - I am pretty satisfied with the results of my experiment as the pigment matches

    the RF value and colour. There were a few errors namely: the colours my eyes

    perceive are not 100% correct (random error), the chromatography paper

    folded and wrinkled in the beaker (random error),

    Bibliography

    1. https://www.researchgate.net/figure/STANDARD-Rf-VALUES-OF-FLOWER-PIGMENTS_tbl4_333053394
    date accessed: 7.10.2021, date created: 1.09.2018

    1
    https://www.researchgate.net/figure/STANDARD-Rf-VALUES-OF-FLOWER-PIGMENTS_tbl4_333053394

    pg. 5

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