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    Phosphorus LS Mono-Test: Molybdate U.V. Method

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    Dinesh Sreedharan
    This document provides instructions for using a phosphorus test reagent kit that uses a molybdate U.V. method to quantitatively determine phosphorus levels. The kit is intended for in vitro use in a clinical laboratory to estimate phosphorus in serum, plasma, or urine. Phosphorus levels are important for bone and cell function. The procedure involves reacting a sample with ammonium molybdate to form a phosphomolybdate complex, which is measured at 340nm. Absorbance readings are used to calculate phosphorus levels based on a standard curve.

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    Phosphorus LS Mono-Test: Molybdate U.V. Method

    Uploaded by

    Dinesh Sreedharan
    17 views1 page
    This document provides instructions for using a phosphorus test reagent kit that uses a molybdate U.V. method to quantitatively determine phosphorus levels. The kit is intended for in vitro use in a clinical laboratory to estimate phosphorus in serum, plasma, or urine. Phosphorus levels are important for bone and cell function. The procedure involves reacting a sample with ammonium molybdate to form a phosphomolybdate complex, which is measured at 340nm. Absorbance readings are used to calculate phosphorus levels based on a standard curve.

    Original Title

    Phosphorus Synergy

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    This document provides instructions for using a phosphorus test reagent kit that uses a molybdate U.V. method to quantitatively determine phosphorus levels. The kit is intended for in vitro use in a clinical laboratory to estimate phosphorus in serum, plasma, or urine. Phosphorus levels are important for bone and cell function. The procedure involves reacting a sample with ammonium molybdate to form a phosphomolybdate complex, which is measured at 340nm. Absorbance readings are used to calculate phosphorus levels based on a standard curve.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    17 views1 page

    Phosphorus LS Mono-Test: Molybdate U.V. Method

    Uploaded by

    Dinesh Sreedharan
    This document provides instructions for using a phosphorus test reagent kit that uses a molybdate U.V. method to quantitatively determine phosphorus levels. The kit is intended for in vitro use in a clinical laboratory to estimate phosphorus in serum, plasma, or urine. Phosphorus levels are important for bone and cell function. The procedure involves reacting a sample with ammonium molybdate to form a phosphomolybdate complex, which is measured at 340nm. Absorbance readings are used to calculate phosphorus levels based on a standard curve.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 1

    Phosphorus LS Mono-test

    Molybdate U.V. Method


    50 x 1 mL
    Store at 2-8C

    Quantitative determination of Phosphors by means of Molybdate U. V. Test.


    Phosphorus in mg/dL = (Abs. T) - (Abs. B) x 5 (Standard)
    Only for in vitro use in clinical laboratory (IVD). (Abs. S) - (Abs. B)
    Application sheets for automated systems are available on request.
    Intended Use: Phosphorus(Inorganic) test reagent/kits is a medical device
    intended for the estimation of Phosphorus(Inorganic) in serum, plasma or
    Calibrators and Controls
    urine.
    It is recommended that appropriate quality control sera and/or controls be run with
    Clinical Significance each assay batch to monitor procedural parameters.
    Phosphorus is an essential mineral for tissue bone formation and is required by every
    cell in the body for normal function. Approximately 85% of the body phosphorus is
    found in bone and in teeth. Low levels of phosphorus can be caused by hyper Reference Range
    vitaminosis D, primary hyper parathyroidism, renal tubular disorders, antacids or
    Serum or plasma
    malabsorption. High levels of phosphorus can be caused by diet, bone metastases,
    Children 4.0 – 6.50 mg/dL
    liver disease, alcohol ingestion, diarrhea and vomiting.
    Adults 2.5 – 5.0 mg/dL
    Urine
    Principle Adults 0.3 – 1.0 gm /24 hours
    When Inorganic phosphorus reacts with ammonium molybdic, in an acidic medium, it
    form Phosphomolybdate complex, which is measured in the U.V. range i.e. 340nm. These values are for orientation purpose; each laboratory should
    The absorbance of this complex is directly proportional to the amount of inorganic establish its own reference range.
    phosphorus present in the sample.
    Performance Characteristics
    Linearity: Up to 20 mg/dL
    Reagents
    Reagent 1: Molybdate Reagent : 50 x 1 mL When values exceed this range the samples should be diluted
    Reagent 2: Phosphorus Standard (05mg/dL) :1 x 2 mL appropriately with Distilled water and the result multiplied by the dilution
    Prewashed yellow Micropipette tips : 50 nos. factor.

    Storage Instructions and Reagent Stability


    The reagent is stable up to the end of the indicated month of expiry, if stored at 2–8 Mode End point
    °C, protected from light and contamination is avoided. Do not freeze the reagents Wavelength (nm) 340 nm

    Reagent Preparation & Stability Sample Volume (l) 10 µL


    Reagent 1 is ready for use. Working Reagent Volume(l) 1000 µL

    Specimen and Stability Lag time (Sec.) 5 sec


    Serum or plasma: Free of hemolysis, should be removed from the clot as quickly as Standard(mg/dl) 5 mg/dl
    possible to avoid elevation of serum phosphorus from hydrolysis or leakage of
    phosphate present in erythrocytes. Incubation Time (sec) 5 minutes
    Urine: acidify the urine with few drops of concentrated HCl and dilute the sample 1/10 Reaction Temperature ( C) R.T.
    with distilled water. Mix. Multiply the result by 10 (dilution factor).
    Reaction Direction Increasing
    Stability in serum /plasma : 7 days at 2 – 8 °C Linearity (mg/dl) 20 mg/dl
    Urine : 10 days at 2 – 8 °C
    Additional Equipment Blank with Reagent
    - Spectrophotometer or colorimeter measuring at 340 nm. Units mg/dl
    - Matched Cuvette 1.0 cm light path.
    - General laboratory equipment

    Assay Procedure Literature


    1. Assay conditions: 1. Goodwin. J.F. (1970) Clin Chem16919) : 776
    Wavelength: . . . . . . . . . . . . . . . . . . . . . . . ... 340 nm
    Cuvette: . . . . . .. . . . . . . . . . . . . . .. . .. 1 cm. light path
    Temperature . . . . . . . . . . . . . . . . . . . .. 37 / 30 / 25ºC For in vitro Diagnostic use only.
    2. Adjust the instrument to zero with distilled water
    3. Pipette into a Cuvette
    Dispense Blank Standard Samples
    Reagent 1000 µL 1000 µL 1000 µL
    Distilled Water 10 µL -
    Sample - - 10 µL
    Standard - 10 µL -
    4. Mix, incubate for 5 min. at Room Temperature.
    5. Read absorbance against Reagent blank.

    Calculation

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