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    A Simple Device For Flash Chromatography

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    NgọcKhánhÔng
    This document summarizes a simple, low-cost device for flash chromatography that addresses common issues students face. The device uses a restrictor, pressure regulator, and mercury reservoir to precisely control air pressure and flow into the column. This allows solvent to be added and fractions collected within 2 hours, making column chromatography more practical for teaching.

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    A Simple Device For Flash Chromatography

    Uploaded by

    NgọcKhánhÔng
    22 views1 page
    This document summarizes a simple, low-cost device for flash chromatography that addresses common issues students face. The device uses a restrictor, pressure regulator, and mercury reservoir to precisely control air pressure and flow into the column. This allows solvent to be added and fractions collected within 2 hours, making column chromatography more practical for teaching.

    Original Title

    JCE 1984, 61, 649

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    This document summarizes a simple, low-cost device for flash chromatography that addresses common issues students face. The device uses a restrictor, pressure regulator, and mercury reservoir to precisely control air pressure and flow into the column. This allows solvent to be added and fractions collected within 2 hours, making column chromatography more practical for teaching.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    22 views1 page

    A Simple Device For Flash Chromatography

    Uploaded by

    NgọcKhánhÔng
    This document summarizes a simple, low-cost device for flash chromatography that addresses common issues students face. The device uses a restrictor, pressure regulator, and mercury reservoir to precisely control air pressure and flow into the column. This allows solvent to be added and fractions collected within 2 hours, making column chromatography more practical for teaching.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    A Simple Device for Flash Chromatography

    Flash chromatography is an important improvement in column chromatography;' use of 40-63 p m silica instead of
    W 2 0 0 pm increases the optimal rate of the mobile phase, and thus so that elution is complete within 20 min. This is specially
    important for teaching, because column chromatography can he very tedious and time-consuming in a practical lab course
    in organic synthesis. With this new technique, preparation of the column, elution, and analysis of the fractions are completed
    in two hours.
    ~ ~ ~~~~

    The only trouble for s t u d ~ n t ais trse of compressed air. The column must he opened and filled with solvent frequently
    and each time the air flow regulator has u,he readjusted. l i t h e pressure is released t m fast, the adsorbent layer is cracked.
    If the pressure is tw high, then the air flow regulat<,r,which is expensive, is often ejected and broken. We descrihe her? a
    simple. low-cost device whwh prevents all these inconveniences.
    Am is admitted into the system through a restrictor. which is specially useful when the pressure available is ton great.
    The restrictor is simdv a 8-mma d . alms tuhe, constricted at oneend in order to h a w a 2-mm hole. This admits only a l w
    air flow into the device. The restrict& is connected by silicone tubing t o the pressure regulator, which is a T tube immersed
    in mercury. Use of a n SVLglass screw adaptor allows a convenient adjustment of the useful height of mercury (a) in order
    t o obtain a mobile phase rate of 5 cmlmin.
    For a. given column diameter (a) needs only t o calibrated once. Excess of sir flow evacuates very safely by bubbling
    through the mercury. T o avoid mercury vapor in the lah, the outlet was connected t o a h w d by rubber tuhing.
    The pressure reservoir that is connected to the pressure regulator is a 3-1 flask or tube, wrapped with a safety grill. Once
    the whole device is a t the right pressure, it is possible to have a very low air flow which bubbles through the mercury. Two
    columns can he connected simultaneously t o the pressure reservoir, if each can be insulated from the device with an ordinary
    glass tap.

    Air comes on the column by a bent pound-glass joint, firmly attached with rubber hands. The shape of the bottom
    of the column and its diameter are identical ta those recommended hv Still. At the too of the column. we added a solvent
    reservoir (500 ml for a 4-cm column, 1 I for n 5-rm rolumnl which allows to complete the whole evolution w ~ t h w opening
    r
    the rolumn. Introduction <,fthe mlwe and the solvent mixture was achieved with a long miled funnel which had n mwtrictpd
    end, to avoid pertwhation of the sand surface ( w e msert in figure).

    ' Still, W. C., Kahn, M., and Mitra, J. J. Olg. Chem.,43,2923 (1979).
    A. Feigenbaum
    bboratoire de Photochemie
    UER Sciences
    ERA 688.51082 Reims. France

    Volume 61 Number 7 July 1964 649

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