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    Ex 7

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    Anuj Pati
    The document summarizes gamete development in mice by describing: 1) Spermatogenesis in the testes, which occurs within seminiferous tubules and involves germ cells developing from spermatogonia through spermatocytes, spermatids, and ultimately spermatozoa. 2) Oogenesis in the ovaries, with follicles at various development stages in the cortex and medulla containing ova surrounded by follicle cells. 3) An experiment to study gamete development through slides of mouse testes and ovaries under a microscope, observing germ cell development in seminiferous tubules and follicles at different stages.

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    Ex 7

    Uploaded by

    Anuj Pati
    150 views3 pages
    The document summarizes gamete development in mice by describing: 1) Spermatogenesis in the testes, which occurs within seminiferous tubules and involves germ cells developing from spermatogonia through spermatocytes, spermatids, and ultimately spermatozoa. 2) Oogenesis in the ovaries, with follicles at various development stages in the cortex and medulla containing ova surrounded by follicle cells. 3) An experiment to study gamete development through slides of mouse testes and ovaries under a microscope, observing germ cell development in seminiferous tubules and follicles at different stages.

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    The document summarizes gamete development in mice by describing: 1) Spermatogenesis in the testes, which occurs within seminiferous tubules and involves germ cells developing from spermatogonia through spermatocytes, spermatids, and ultimately spermatozoa. 2) Oogenesis in the ovaries, with follicles at various development stages in the cortex and medulla containing ova surrounded by follicle cells. 3) An experiment to study gamete development through slides of mouse testes and ovaries under a microscope, observing germ cell development in seminiferous tubules and follicles at different stages.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    150 views3 pages

    Ex 7

    Uploaded by

    Anuj Pati
    The document summarizes gamete development in mice by describing: 1) Spermatogenesis in the testes, which occurs within seminiferous tubules and involves germ cells developing from spermatogonia through spermatocytes, spermatids, and ultimately spermatozoa. 2) Oogenesis in the ovaries, with follicles at various development stages in the cortex and medulla containing ova surrounded by follicle cells. 3) An experiment to study gamete development through slides of mouse testes and ovaries under a microscope, observing germ cell development in seminiferous tubules and follicles at different stages.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 3

    MENT FOR Sp Study of Gamete

    SPO Development in
    11 Mouse (Mammal)
    INTRODUCTION

    Gametes are the sex cells involved in the process of sexual reproduction.
    The gametes differ from all other cells (= somatic cells) of the body in that their nuclei contain
    only half the number of chromosomes found in the nuclei of somatic cells.

    Meiosis forms the most significant part of process of


    gametogenesis.
    4 Gametogenesis for the formation of sperms is termed spermatogenesis, while that of ova is
    called oogenesis.
    Spermatogenesis occurs in the seminiferous tubules of the testes, whereas oogenesis occurs in
    the ovaries.

    EXPERIMENT 11.1
    AIM: To study and identify the stages
    of ganete
    development in mouse (mammal) i.e., T.S. of
    testis and L.S. of ovary through permanent slide.

    REQUIREMENTS
    Permanent slide of T.S. of testis and L.S. of ovary,
    microscope.
    PROCEDURE
    Fix the permanent slide under the microscope. First observe it under the low power and then
    under high power.

    OBSERVATION
    T.S. of Testis
    The testis of a mouse (mammal) is covered by a thick fibrous tissue called tunica albuginea.
    Ihe testis consists of numerous seminiferous tubules embedded in the interstitial tissue.

    Various types of germinal cells are present from outside towards lumen in the following sequence.
    permatogonia > Spermatocytes Spermatids Spermatozoa - Sperms.
    Between the germinal cells, pyramid shaped cells called sertoli cells are present.
    Comprehensiue boratory Manual in Biolog
    66
    heads
    embedded in ells are
    sertoli cells Ars

    with their present in the


    Alarge number of
    spermatozoa

    lumen of seminiferous tubule.


    which produce
    male sex
    rmone ttestosterone
    hormone
    Ihe interstitial tissue also contain leydigs cells,
    Visceral
    peritoneum
    Tunica
    albuginea
    Blood vessel Spermatozoa
    Seminiferous Spermatid
    tubule
    o
    1000oa
    Secondary
    spermatocyte
    Sertoli cell
    Primary
    spermatocyte
    Connective
    tissue Sertoli cell
    Germinal epithelium
    Spermatogonia
    Spermatocytes Spermatogonium
    Spermatids
    Spermatozoa
    O
    Interstitial
    A cells B

    Fig.11.1. A. A Part oftransverse section oftestis of mouse(mammal)


    B. Sectional view of a part of seminiferous tubule (enlarged)

    V.S. of Ovary
    A mouse ovary is a solid structure bounded by germinal epithelium followed by a thick layer of
    fibrous tissue, the tunica albuginia.
    Egg nest
    Cortex Primary follicle
    Seconday
    Bloobd follicle
    vessel
    Tertiary
    follicle

    Mesovarium Visceral
    peritoneum

    Graafian follicle
    Corpus
    albicans
    o

    Corpus luteum

    Ruptured follicle
    Medulla
    Fig. 11.2. A section of
    ovary of mouse (mammal)
    o r t yermmeits 67
    ovary consists of outer cortex and inner medulla
    The

    e iedulla contains many rounded or oval bodien called ovarlan or Graafanfollicles at varius

    stages of development

    he medulla also contains blood vesaels, nerves fibren and nome smooth muscles.
    Bach follicle contains a large ovum aurrounded by many layers of follile cell
    contains young and mature follicles.
    6The cortex
    ,The cortex may
    also contain a large mass of yellow cells termed corpus luteum, forrmed in an

    follicle after the release of its ovum.


    empty Graafian

    PRECAUTIONS
    Eirst observe the slide under low power and then under the high power of the microscope
    2 Use fine adjustment of the microscope for focussing the slide under high power.

    VIVA VOCE
    ?
    Q1. What is gametogenesis
    and
    Gametogenesis is the process by which male and female sex cells gametes i.e., sperms
    or
    Ans.
    ova are formed respectively in the testes and ovaries.
    Q2. What is spermatogenesis ?
    Ans. The process of formation of sperms in the testis is called spermatogenesis.

    .3. What is oogenesis?


    Ans. The process of formation of ova in the ovary is called oogenesis.

    Q4. What are the main parts of a spermatozoa ?


    Ans. A typical mammalian spermatozoa consists of a head, neck, middle piece and tail.
    . At what stage does the human ovum is released from the ovary ?

    Ans. The ovum is released from the ovary in the secondary oocyte stage

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