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    DSEBT-691A Lab 2

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    Rakshit Mittal
    The document describes an experiment to use the xanthoproteic test to detect aromatic amino acids like tyrosine and tryptophan. The test involves adding concentrated nitric acid and sodium hydroxide to a sample solution. If aromatic amino acids are present, a dark yellow or orange color change will occur due to the formation of nitro derivatives and xanthoproteic acid salts. The test is qualitative and only indicates the presence or absence of aromatic amino acid groups.

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    DSEBT-691A Lab 2

    Uploaded by

    Rakshit Mittal
    28 views3 pages
    The document describes an experiment to use the xanthoproteic test to detect aromatic amino acids like tyrosine and tryptophan. The test involves adding concentrated nitric acid and sodium hydroxide to a sample solution. If aromatic amino acids are present, a dark yellow or orange color change will occur due to the formation of nitro derivatives and xanthoproteic acid salts. The test is qualitative and only indicates the presence or absence of aromatic amino acid groups.

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    GENETIC MODIFICATION

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    The document describes an experiment to use the xanthoproteic test to detect aromatic amino acids like tyrosine and tryptophan. The test involves adding concentrated nitric acid and sodium hydroxide to a sample solution. If aromatic amino acids are present, a dark yellow or orange color change will occur due to the formation of nitro derivatives and xanthoproteic acid salts. The test is qualitative and only indicates the presence or absence of aromatic amino acid groups.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    28 views3 pages

    DSEBT-691A Lab 2

    Uploaded by

    Rakshit Mittal
    The document describes an experiment to use the xanthoproteic test to detect aromatic amino acids like tyrosine and tryptophan. The test involves adding concentrated nitric acid and sodium hydroxide to a sample solution. If aromatic amino acids are present, a dark yellow or orange color change will occur due to the formation of nitro derivatives and xanthoproteic acid salts. The test is qualitative and only indicates the presence or absence of aromatic amino acid groups.

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    Guru Nanak Institute of Pharmaceutical Science and Technology

    Sub: Lab on Genetic Modification in Agriculture and Medicine Paper code: DSEBT-691A
    Experiment No: 2 Date:

    AIM – DETERMINATION OF AMINO ACIDS USING XANTHOPROTEIC TEST

    OBJECTIVE:

    • To detect the presence of aromatic groups-containing amino acids like tyrosine and
    tryptophan.
    • To differentiate tyrosine and tryptophan from other amino acids.

    PRETEXT QUESTIONS:

    1. Xanthoproteic test is performed to identify which of the following amino acid?


    a) Tryptophan b) Glycine c) Arginine d) Histidine

    2. What is the percentage of NaOH which is used in xanthoproteic test?


    a) 20% b) 30% c) 40% d) 50%

    PRINCIPLE:

    Xanthoproteic test is a biochemical test for the detection of amino acids containing phenolic
    or indolic groups like phenylalanine, tyrosine, and tryptophan (aromatic amino acids). The
    test is named Xanthoproteic test due to the formation of a yellow precipitate of xanthoproteic
    acid. The term ‘Xantho’ refers to ‘yellow’, so the test is often termed as the Yellow Protein
    Test. The test gives a positive result for amino acids containing benzene rings or other
    aromatic groups. The test is a qualitative test that provides information only on the presence
    or absence of the amino acids.

    The Xanthoproteic test is based on the fact that aromatic groups in the amino acids are
    nitrated by heating with concentrated HNO3 to yield intensely yellow-colored nitro
    derivative. On the addition of alkali, however, the residue turns orange due to the formation
    of a salt of the tautomeric form of the nitro compound. Benzene ring-containing amino acids
    like phenylalanine don’t give a positive test to this test because the phenyl group in
    phenylalanine is very stable, which doesn’t react with nitric acid in the conditions of this test.
    However, phenylalanine might give a positive result after an extended period of heating.
    Guru Nanak Institute of Pharmaceutical Science and Technology
    Sub: Lab on Genetic Modification in Agriculture and Medicine Paper code: DSEBT-691A
    MATERIALS REQUIRED:

    Reagent
    • Concentrated Nitric acid
    • 40% NaOH
    • Test solution

    Material Required
    • Test tubes
    • Test tube stand
    • Pipettes

    METHODOLOGY:

    1. About 1 ml of the sample solution is taken in a test tube. To this, the same amount of
    concentrated nitric acid is added.
    2. The test tube is allowed to cool down to room temperature. If the sample is a protein
    solution, a white precipitate might develop due to the denaturation of proteins.
    3. Then, 1 ml of 40% NaOH solution is added to the test tube and observed for color change.

    RESULT AND OBSERVATION:

    • Positive result: The appearance of a dark yellow or orange-colored solution represents a


    positive test. This indicates the presence of aromatic groups in the proteins and amino
    acids.
    • Negative result: The absence of a dark yellow or orange-colored solution represents a
    negative test. This indicates the absence of aromatic groups in proteins and amino acids.
    Guru Nanak Institute of Pharmaceutical Science and Technology
    Sub: Lab on Genetic Modification in Agriculture and Medicine Paper code: DSEBT-691A

    CRITICAL THINKING QUESTIONS:

    1. What is the colour of protein in Xanthoproteic test?

    2. What is the mechanism of Xanthoproteic test?

    ATTENDANC VIVA PERFORMANCE TOTAL

    E(10) (10) (20) (40)

    CLEANLINESS CONDUCT PRACTICAL CRITICAL


    & MANUAL & ACCURACY THINKING
    MAINTENANCE INVOLVEMENT (4) (8)
    (4)
    (4)

    Signature of the Teacher

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