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Quarter 3 Biotech 2

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BIOLOGY

RD
3 QUARTER

THE FLOW OF THE GENETIC INFORMATION FROM DNA TO RNA TO PROTEIN


GENETIC INFORMATION IN DNA- is a living archive of instructions that cells use to
accomplish the functions of life.
PROTEINS- make up the structures of the cell

HOW IS GENETIC INFORMATION PASSED ON IN DIVIDING CELLS?


DNA REPLICATION- is the process by which DNA makes a copy of itself during cell division.
When a cell divides, it creates one copy of its genetic information- in the form of DNA
molecules – for each of the two resulting daughter cells.
- It should be accurate as possible
DOUBLE-HELICAL STRUCTURE OF DNA- help ensure the precise replication
- It is made up of combinations of molecules called nucleotides
- ADENINE
- THYMINE
- CYTOSINE
- GUANINE
COMPLEMENTARY BASE-PAIRING- a pattern when the nucleotides that form one strand off
the DNA double helix always bond with the nucleotides in the other strand.

THREE GENERAL CLASSES OF RNA


mRNA (messenger RNA)- molecules carry the coding sequences for protein synthesis and
are called transcripts
rRNA (ribosomal RNA)- molecules from the core a cell’s ribosome (the structures in which
protein synthesis takes place)
- Sometimes called a ribozyme or catalytic RNA
tRNA (transfer RNA)- molecules carry amino acids to the ribosomes during protein synthesis

RIBOSOMES- are the sites in a cell in which protein synthesis takes place.
BIOLOGY
RD
3 QUARTER

CELLS- have many ribosomes, and the exact number depends on how active a particular cell
is in synthesizing proteins.

INITIAL STEPS IN ACCESSING GENETIC INFPORMATION


1st step- TRANSCRIPTION
- During transcription enzymes called RNA Polymerases build RNA molecules that
are complementary to a portion of one strand of the DNA double helix.
3 steps
1. INITIATION- is the attachment of RNA Polymerase to the promoter and
the start of RNA synthesis.
2. ELONGATION- - RNA grows longer. This is called RNA synthesis.
3. TERMINATION- RNA Polymerase reaches a special sequence of bases in
the DNA template called a terminator
2nd step- TRANSLATION- or the reading of these mRNAs to make proteins begin.
- It occurs at the ribosomes, which consist of rRNA and proteins
- The instructions in mRNA are read, and tRNA brings the correct sequence of
amino acids to the ribosome. Then rRNA helps bonds form between the amino
acids, producing a polypeptide chain.
- After a polypeptide chain is synthesized, it may undergo additional processing to
form finish protein.

HOW DOES A MUTATION IN DNA AFFECTS AN INDIVIDUAL?


MUTATION- is a changed in DNA sequence brought about either by mistake made when the
DNA is copied or through chemical change.
GENES- provide instructions for creation of protein molecules, which perform most of the
important jobs in cells.
BIOLOGY
RD
3 QUARTER

BIOTECHNOLOGY- the science of using the living systems to benefit humankind.


GENETIC ENGINEERING- involves the use of:
RECOMBINANT DNA TECHNOLOGY- the process by which the DNA sequence is manipulated
IN VITRO, thus creating RECOMBINANT DNA MOLECULES, that have new combinations of
genetic material. The recombinant DNA is then introduced into a host organism. If the DNA
that is introduced comes from a different species, the host organism is now considered
TRANSGENIC.

TOOLS OF RECOMBINANT DNA TECHNOLOGY


1. RESTRICTION ENZYMES or RESTRICTION ENDONUCLEASES- cut DNA at or near
specific nucleotide sequence
2. DNA LIGASE- joins fragments of DNA together
3. DNA POLYMERASE- catalyzes the synthesis of DNA; RNA POLYMERASE- catalyzes the
synthesis of RNA
4. GEL ELECTROPHORESIS- separates charged molecules based on charge (neutral,
positive, and negative) and size. Charged molecules move on a medium to the
opposite pole when current is applied.
5. POLYMERASE CHAIN REACTION (PCR)- is a technology which allows the amplification
of parts of DNA by in vitro replication that involved repeated denaturation, primer
attachment and synthesis of DNA.

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