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Lecture 5 - Environmental Microbiology and Parasitology

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Lecture 5: Environmental

Microbiology and Parasitology


in Environmental Engineering
Environmental microbiology

• the study of microbial processes in the


environment, microbial communities and
microbial interactions
• Also called microbial ecology -
the ecology of microorganisms, their
relationship with one another and with their
environment
Environmental microbiology coverage

• Structure and activities of microbial communities


• Microbial interactions and interactions with
macroorganisms
• Population biology of microorganisms
• Microbes and surfaces (adhesion and biofilm formation)
• Microbial community genetics and evolutionary
processes
• (Global) element cycles and biogeochemical processes
• Microbial life in extreme and unusual little-explored
environments
Key points on microorganisms
• Microbes are everywhere in the biosphere
• The effects of microorganisms on their environment can be beneficial or harmful
• Microorganisms play a major role in maintaining the Earth’s ecosystem
• Microorganisms exist as unicellular, multicellular, or cell clusters
• Microorganisms are divided into seven types: bacteria, archaea, protozoa, algae, fungi, viruses, and
multicellular animal parasites (helminths )
• Microorganisms are beneficial in producing oxygen, decomposing organic material, providing nutrients for
plants, and maintaining human health
• Some microorganisms can be pathogenic and cause diseases in plants and humans
• Prokaryotes are organisms whose cells lack
a nucleus and other organelles.

• Eukaryotes have a membrane-bound


nucleus
Types of Microorganisms
Bacteria
• Shapes
-Bacillus (rod shape), coccus (spherical shape), spirilla (spiral
shape), vibrio (curved shape); other shapes
• Cell wall structure stains
-Gram-positive or Gram-negative
• Response to gaseous oxygen
- aerobic (living in the presence of oxygen), anaerobic (living
without oxygen), and facultative anaerobes (can live in both
environments)
• Obtaining energy
- Autotrophs make their own food by using the energy of
sunlight
- Chemoautotrophs make their own food by using chemical
reactions
- Heterotrophs obtain their energy by consuming other
organisms.
- Saprophytes use decaying life forms as a source of energy
• Capsule: A layer found on the outside of the cell wall in some
Bacteria Structure bacteria.
• Cell wall: A layer that is made of a polymer called peptidoglycan.
The cell wall gives the bacteria its shape. It is located outside the
plasma membrane . The cell wall is thicker in some bacteria, called
Gram positive bacteria.
• Plasma membrane: Found within the cell wall, this generates
energy and transports chemicals. The membrane is permeable,
which means that substances can pass through it.
• Cytoplasm: A gelatinous substance inside the plasma membrane
that contains genetic material and ribosomes.
• DNA: This contains all the genetic instructions used in the
development and function of the bacterium. It is located inside
the cytoplasm.
• Ribosomes: This is where proteins are made, or synthesized.
Ribosomes are complex particles made up of RNA-rich granules.
• Flagellum: This is used for movement, to propel some types of
bacteria. There are some bacteria that can have more than one.
• Pili: These hair-like appendages on the outside of the cell allow
it to stick to surfaces and transfer genetic material to other cells.
This can contribute to the spread of illness in humans
Archaea
• Similar to bacteria, archaea do not have interior membranes but both have a
cell wall and use flagella to swim.
• Archaea differ in the fact that their cell wall does not contain peptidoglycan
and cell membrane uses ether linked lipids as opposed to ester linked lipids in
bacteria.
• They are prokaryotic cells with avidity to extreme environmental conditions.
• They absorb sunlight using their membrane pigment, bacteriorhodopsin
which leads to the formation of the energy molecule adenosine triphosphate
(ATP)
• They use different energy sources like hydrogen gas, carbon dioxide, and
sulphur.
• Groups
✓ Methanogens - methane-producing organisms
✓ Halophiles - live in salty environments
✓ Thermophiles -live at extremely hot temperatures
✓ Psychrophiles - live at extremely cold-temperature
Protozoa
• Protozoa are unicellular aerobic eukaryotes
• They have a nucleus, complex organelles, and obtain
nourishment by absorption or ingestion through
specialized structures.
• Their cell walls are made up of cellulose.
• Groups:
✓ Flagellates- produce their own food and use their
whip-like structure to propel forward
✓Ciliates have tiny hair that beat to produce
movement
✓Amoeboids have false feet or pseudopodia used
for feeding and locomotion
✓Sporozoans are non-motile
Algae
• Also called cyanobacteria or blue-green algae
• Unicellular or multicellular eukaryotes that obtain
nourishment by photosynthesis.
• They live in water, damp soil, and rocks and produce
oxygen and carbohydrates used by other organisms.
• The 11 types of algae are as follows: Chlorophyceae
(Green algae), Phaeophyceae (Brown
Algae), Rhodophyceae (Red Algae), Xanthophyceae
(Yellow-Green Algae), Chrysophyceae (Golden
Algae), Bacillariophyceae (Diatoms), Cryptophyceae,
Dinophyceae, Chloromonadineae, Euglenineae, and
Cyanophyceae or Myxophyceae (Blue-green algae).

Ref: https://microbiologynote.com/types-of-algae/
Fungi
• Fungi (mushroom, molds, and yeasts) are eukaryotic cells (with
a true nucleus)
• Most fungi are multicellular and their cell wall is composed of
chitin.
• They obtain nutrients by absorbing organic material from their
environment (decomposers), through symbiotic relationships
with plants (symbionts), or harmful relationships with a host
(parasites)
• Divisions
Chytridiomycota (chytrids); Zygomycota (bread
molds), Ascomycota (yeasts and sac fungi),
Glomeromycota, and the Basidiomycota (club fungi)
Virus
• Viruses are noncellular entities that consist
of a nucleic acid core (DNA or RNA)
surrounded by a protein coat called capsid
• Around the capsid, there may be a spiky
covering known as the envelope. These
spikes are proteins that enable viruses to
bind to and enter host cells.
• Viruses cannot reproduce outside a host cell
and cannot metabolize on their own.
• Viruses often infest prokaryotic and
eukaryotic cells causing diseases.
• Shapes: helical, polyhedral, spherical, and
complex
Multi-cellular animal parasites (helminths)
• A group of eukaryotic organisms consisting of the
flatworms and roundworms, collectively referred to as
the helminths.
• Although they are not microorganisms by definition,
since they are large enough to be easily seen with the
naked eye, they live a part of their life cycle in
microscopic form.
• Since the parasitic helminths are of clinical importance,
they are often discussed along with the other groups
of microbes.
• They are included in the field of microbiology because
their eggs and larvae are often microscopic.
• The major groups of parasitic helminths include:
platyhelminths (flatworms) acanthocephalins (thorny-
headed worms); cestodes (tapeworms);
trematodes (flukes) and nematodes (roundworms)
Parasitology
• The study of parasites, their hosts, and the relationship between
them
• Environmental parasitology - deals with the interactions between
parasites and pollutants in the environment
• Key parasites covered by environmental microbiology
-Protozoas
-Helminths
-Fungi
-Viruses
Benefits and risks of microbes
Benefits
-Symbiosis with plants and animals (e.g. lactobacilli)
-Production of food and fuel (e.g. yogurt, cheese, vinegar, gasohol)
- Medical, pharmaceutical and biotechnological applications (e.g. antibiotics,
vaccines, medicines)
- Nutrient cycling, decomposition of wastes, source of energy, bioremediation

Risks
- Infectious diseases and deaths (e.g. Covid-19, HIV/AIDS, diarrhea)
- Food spoilage and decomposition
- Nuisance in the operation of facilities (e.g. iron bacteria in water pipes)
Application in Environmental Engineering

Environmental engineers are interested in microorganisms for at least three different


reasons:
• harmful microorganisms cause disease and need to be eliminated to protect public health
(disinfection, sanitizing, sterilization)
• useful microorganisms as food/medicines; catalysts in cleaning the environment
(bioremediation, wastewater treatment, water treatment); maintaining natural cycles
• nuisance microorganisms hinder the stable operation of environmental engineering
systems and need to be monitored and controlled (environmental monitoring)
Nuisance
organisms

Can clog

Pipes

Cleaning

Wastewater
treatment

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