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    Laboratory Activity Group 7

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    JESSEL
    This document outlines an activity to enumerate microorganisms from the environment. Students will culture samples from various surfaces like door knobs, hair, shoes, and cellphones in petri dishes containing agar gelatin medium. The dishes will be incubated to allow bacterial colonies to form. Students will then draw the colonies and observe cells under a microscope. Enumerating microorganisms in this way allows researchers to quantify bacterial loads in different settings and identify the types of bacteria present, gaining insights into microbial diversity and distribution in various habitats.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Laboratory Activity Group 7

    Uploaded by

    JESSEL
    8 views3 pages
    This document outlines an activity to enumerate microorganisms from the environment. Students will culture samples from various surfaces like door knobs, hair, shoes, and cellphones in petri dishes containing agar gelatin medium. The dishes will be incubated to allow bacterial colonies to form. Students will then draw the colonies and observe cells under a microscope. Enumerating microorganisms in this way allows researchers to quantify bacterial loads in different settings and identify the types of bacteria present, gaining insights into microbial diversity and distribution in various habitats.

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    Laboratory-Activity-Group-7

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    This document outlines an activity to enumerate microorganisms from the environment. Students will culture samples from various surfaces like door knobs, hair, shoes, and cellphones in petri dishes containing agar gelatin medium. The dishes will be incubated to allow bacterial colonies to form. Students will then draw the colonies and observe cells under a microscope. Enumerating microorganisms in this way allows researchers to quantify bacterial loads in different settings and identify the types of bacteria present, gaining insights into microbial diversity and distribution in various habitats.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    8 views3 pages

    Laboratory Activity Group 7

    Uploaded by

    JESSEL
    This document outlines an activity to enumerate microorganisms from the environment. Students will culture samples from various surfaces like door knobs, hair, shoes, and cellphones in petri dishes containing agar gelatin medium. The dishes will be incubated to allow bacterial colonies to form. Students will then draw the colonies and observe cells under a microscope. Enumerating microorganisms in this way allows researchers to quantify bacterial loads in different settings and identify the types of bacteria present, gaining insights into microbial diversity and distribution in various habitats.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Republic of the Philippines

    BICOL STATE COLLEGE OF APPLIED SCIENCES AND TECHNOLOGY


    Naga City

    Course Title: GENERAL MICROBIOLOGY

    Course Code: FTECH-101

    Professor: ARNEL S. JARDINEL, MAEd Sc

    Name of Students: ECO, ALEXIS │ESTRELLA, NICKANNE KIRK

    Term: SECOND SEMESTER, A/Y 2022-2023

    ACTIVITY

    ENUMERATION OF MICROORGANISMS FROM THE ENVIRONMENT

    Microorganism enumeration from the environment is a scientific activity that aims to measure and identify the

    bacterial load or concentration present in a particular sample. This method enables researchers to acquire

    significant insights on the microbial composition of different settings as well as analyze the diversity and features

    of bacterial strains.

    Scientists obtain significant insights into the microbial world through the enumeration of microorganisms from the

    environment, which contributes to sectors such as environmental monitoring, public health, and microbiology

    research. This activity promotes a greater understanding of the diversity and dispersion of bacteria in various

    habitats, ultimately leading to breakthroughs in several scientific areas.

    I. Objectives:

    ● To quantify the bacterial load or concentration present in the sample.


    ● To identify the bacteria, present in the sample.
    ● To identify and classify bacterial strains present in the sample.

    II. Materials:

    1 tbsp unflavored gelatin (White)


    500 mL distilled water
    Sterilized Petri Dish

    1
    ¼ Beef broth cube
    III. Procedures:

    PART I. CULTURE MEDIA


    1. Prepare a petri dish with agar medium (sterilized petri dishes)
    2. In the beaker, combine 1 tablespoon gelatin and ¼ broth cube.
    3. Stir occasionally until boil
    4. Put a label in the petri dish (A, B, C, D, E, F)
    o Petri Dish A: Exposed to the air for 5 minutes
    o Petri Dish B: Use cotton swab to wipe an area of door knob and
    streak onto entire surface
    o Petri Dish C: Fingers were touched to the hair for 5 seconds and
    streak to the gelatin
    o Petri Dish D: Coughed in the agar
    o Petri Dish E: Fingers were touched to the shoes for 5 seconds and
    streak to the gelatin
    o Petri Dish F: Use cotton swab to wipe an area of cellphone and
    streak to the gelatin
    5. Cover the lid and incubate the culture. Let colonies form.
    6. Place the slide under the microscope and observe the bacteria.

    PART II. DRAWING AND MICROSCOPY

    1. Draw the colonies that formed in culture media.

    2. Microscopy: Draw the microbial cell/s present in a colony.

    2
    IV. Activity proper:

    V. Reference
    Jhonamie Mabuhay. (2020, November 25). Enumeration of microorganisms
    from the environment.

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