Chapter 11 Biological Membrane

and Transport

11.1 The Composition and

Architecture of Membranes
11.2 Membrane Dynamics
11.3 Solute Transport across
Membranes

p.371

What are Membranes?

‡Composed of a variety of lipids and proteins

‡Some membrane lipids and proteins are
glycosylated
‡All cells have the cell membrane, which separates
the cell from its surrounding

1
 Functions of Membranes
‡ Define the boundaries of the cell
‡ Allow import and export
„ Selective import of nutrients (e.g. lactose)
„ Selective export of waste and toxins (e.g. antibiotics)
‡ Retain metabolites and ions within the cell
‡ Sense external signals and transmit information into the cell
‡ Provide compartmentalization within the cell
„ separate energy-producing reactions from energy-consuming
ones
„ keep proteolytic enzymes away from important cellular
proteins

11.1 The Composition and

Architecture of Membranes
Each Type of Membrane Has Characteristic Lipids and
Proteins

‡ Cells clearly have mechanisms to control the kinds

and amounts of membrane lipid they synthesize
and to target specific lipids to particular
organelles.
‡ Plasma membranes are enriched in cholesterol
and contain no detectable cardiolipin (Fig. 11–2).

p.372

2
FIGURE 11-1

FIGURE 11–1 Biological membranes.

p.371

TABLE 11-1

p.372

3
FIGURE 11-2

FIGURE 11–2 Lipid composition of the plasma membrane and organelle

membranes of a rat hepatocyte.
p.372

All Biological Membranes Share Some Fundamental

Properties
‡ Fluid mosaic model for the structure of biological
membranes:
‡ Phospholipids and sterols form a bilayer in which the
nonpolar regions of the lipid molecules in each layer face
the core of the bilayer and their polar head groups face
outward, interacting with the aqueous phase on either side.
‡ Proteins are embedded in this bilayer sheet, held by
hydrophobic interactions between the membrane lipids
and hydrophobic domains in the proteins.
‡ Membrane is fluid (lipid and protein molecules free to
move laterally in the plane of membrane)

p.373

4
FIGURE 11-3

FIGURE 11–3 Fluid mosaic model for membrane structure.

p.373

A Lipid Bilayer Is the Basic Structural Element of

Membranes
Three types of lipid aggregate:
(1) Micelles:
„ Forms in the solution of amphipathic molecules that have
larger head than tail
• Fatty acids
• Sodium dodecyl sulfate
„ Each micelle has from a few dozen to few thousand lipid
molecules
„ Aggregation occurs when the concentration of molecules is
higher than a certain threshold
(2) Bilayer:
in which two lipid monolayers (leaflets) form a two
dimensional sheet.
p.374

5
 (3) Vesicle (Lyposome):

‡Small bilayers will spontaneously seal into spherical

vesicles
‡Vesicle membranes can contain artificially inserted
proteins
‡The central aqueous cavity can enclose dissolved molecules
‡They are useful artificial carriers of molecules (e.g. drugs)
‡Vesicles fuse readily with cell membranes or with each
other

p.374

FIGURE 11-4

vesicle: the edge of

lipid bilayer contacts
Micelles: bilayer: phospholipids
with H2O
lysophospholipids (glycerophospholipids
(phospholipids lacking and sphingolipids)
unstable
one fatty acid), SDS
form vesicles
FIGURE 11–4 Amphipathic lipid aggregates that form in water.
p.374

6
 Common Features of Membranes
‡ Sheet-like flexible structure, 30-100 Å (3-10 nm) thick
‡ Main structure is composed of two leaflets of lipids (bilayer)
„ Except of archaebacteria: monolayer of bifunctional lipids
‡ Form spontaneously in aqueous solution and are stabilized by
non-covalent forces, esp. hydrophobic effect
‡ Protein molecules span the lipid bilayer
‡ Asymmetric
„ Some lipids are found preferably “inside”
„ Some lipids are found preferably “outside”
„ Carbohydrate moieties are always outside the cell
„ Electrically polarized (inside negative ~ -60mV)
‡ Fluid structures: 2-dimensional solution of oriented lipids

7
 Functions of Proteins in Membranes

‡ Receptors: detecting signals from outside

„ Light (opsin)
„ Hormones (insulin receptor)
„ Neurotransmitters (acetylcholine receptor)
„ Pheromones (taste and smell receptors)
‡ Channels, gates, pumps
„ Nutrients (maltoporin)
„ Ions (K-channel)
„ Neurotransmitters (serotonin reuptake protein)
‡ Enzymes
„ Lipid biosynthesis (some acyltransferases)
„ ATP synthesis (F0F1 ATPase/ATP synthase)

Three Types of Membrane Proteins Differ in

Their Association with the Membrane
‡ Integral membrane proteins are very firmly
associated with the lipid bilayer, and are
removable only by agents that interfere with
hydrophobic interactions.
‡ Peripheral membrane proteins associate with the
membrane through electrostatic interactions and
hydrogen bonding with the hydrophilic domains
of integral proteins and with the polar head
groups of membrane lipids.
‡ Amphitropic proteins are found both in the
cytosol and in association with membranes.

p.375

8
 Peripheral membrane proteins are easily
solubilized
‡ Peripheral membrane proteins: can be relieved
by mild conditions, e.g. change pH or ionic
strength, add urea. If covalently linked to
membrane lipid, use phosphilipase C to cleave the
linkage
‡ Integral membrane proteins: use detergent.

FIGURE 11-6

FIGURE 11–6 Peripheral, integral, and amphitropic proteins.

p.375

9
 extracellular
domain

Transmembrane
domain

intracellular
domain

FIGURE 11–7 Transbilayer disposition of glycophorin in an

erythrocyte. One hydrophilic domain, containing all the sugar residues,
is on the outer surface.

Integral Proteins Are Held in the Membrane

by Hydrophobic Interactions with Lipids
‡ The firm attachment of integral proteins to
membranes is the result of hydrophobic
interactions between membrane lipids and
hydrophobic domains of protein (Figure 11-
8 ).

p.376

10
FIGURE 11-8 are held in the membrane by
Integral Proteins
hydrophobic interactions with lipidsIntegral membrane
proteins
FIGURE 11–8 Integral membrane
proteins. Integral proteins can be
classified into 6 categories:
Type I and II: one transmembrane helix.
Type III: multi-transmembrane helices
in a single polypeptide chain
Type IV: transmembrane domains from
different polypeptides assemble to form
a channel
Type V: held to the bilayer through
covalently linked lipids
Type VI:contain both transmembrane
helices and GPI-anchor

p.377

Bacteriorhodopsin: light-driven proton pump

seven transmembrane
domains
(each ~ 20 a.a., make an a-
helix that spans the bilayer-
9

FIGURE 11–9 Bacteriorhodopsin, a membrane-spanning protein.

p.377

11
 phosphoglycerol
(PG) filled in the
central cavity
a shell of
phospholipids

FIGURE 11–10 Lipid annuli associated with two integral

membrane proteins. (a) sheep aquaporin
(b) Fo integral protein complex of V-type Na+-ATPase: 10
identical subunits, each contains 4 transmembrane helices p.377

The topology of an integral protein can be

sometimes be predicted from its sequence
‡ Lipid bilayer thickness ~ 30Å
‡ A-helix: 5.4 Å/patch and 3.6 residues/turn
‡ 30Å / 5.4 Å x 3.6 residues = 20 residues (per
transmembrane helix)

‡ Hydropathy Index: the relative polarity of each

amino acid side chain determined by measuring the
free energy change accompanying the movement of an
amino acid from a hydrophobic solvent to water
(Table 3-1).

12
To predict the secondary structure of a
transmembrane protein

(1) Scan the hydropathy index along the sequence

(2) Choose the “window” size (can be 7-20 residues)
(3) 將平均hydropathy index 置於中間
e.g. choose window size 7
sequence 1 ~ 7 將平均hydropathy index置於 4
sequence 2 ~ 8 將平均hydropathy index置於 5
sequence 3 ~ 9 將平均hydropathy index置於 6
(4) A region with more than 20 residues of high
average hydropathy index is a “transmembrane
helix domain”

13
 ‡ A further remarkable feature of many
transmembrane proteins of known structure is the
presence of Tyr and Trp residues at the interface
between lipid and water (Fig. 11–12).
‡ Not all integral membrane proteins are composed of
transmembrane helices. Another structural motif
common in bacterial membrane proteins is the β
barrel, in which 20 or more transmembrane segments
form sheets that line a cylinder (Fig. 11–13).

p.379

FIGURE 11-12

‡ Tyr (in orange) and Trp (red) residues of membrane proteins

clustering at the interface between water and lipid. The side
chains of these residues serve as membrane interface anchors
‡ Positive-inside rule: the positively charged Lys, Arg, His
residues (blue) more commonly on the cytoplasmic face of the
membrane

FIGURE 11–12 Tyr and Trp residues of membrane proteins

clustering at the water-lipid interface.
p.379

14
 β Barrel Integral Membrane Proteins

(1) Like α-helical transmembrane domain, β-sheet

transmembrane domain form due to : no water
molecules to form H-bond with the polar C=O
and NH bonds in the hydrophobic environment.
(2) Every second residue is hydrophobicresidue
(recall the structure of β-sheet )
(3) Hydropathy index can not used in the β-barrel
membrane domain prediction

FIGURE 11-13

FIGURE 11–13 Membrane proteins with β--barrel structure.

(1) Porins, proteins that allow certain polar solutes to cross
the outer membrane of gramnegative bacteria such as E.
coli, have many-stranded barrels lining the polar
transmembrane passage.
(2) 20 or more anti-parallel β-strands form a
transmembrane channel
(3) Only 7-9 residues in β-structure are needed to span a
membrane
p.379

15
 Covalently Attached lipids anchor some perpheral
membrane proteins
(1) Some membrane proteins contain one or more covalently
linked lipids including long chain fatty acids, isoprenoids,
GPI (acted as hydrophobic anchor into membrane)
‡ Inner phase of plasma membrane:
(a) Fatty acid 接在 Cys (Ser) side chain上 S (O)上
e.g. palmitoyl group is attached to a Cys residue by
thioester linkage
(b) an N-myristoyl group is generally attached to an
amino-terminal Gly
(c) the farnesyl and geranylgeranyl groups attached to
carboxyl-terminal Cys residues are isoprenoids of 15
and 20 carbons, respectively.

(3) extracellular face of the plasma membrane :

Fatty acid 接在 :
Glycosyl phosphatidylinositol (GPI) anchors are
derivatives of phosphatidylinositol in which the inositol
bears a short oligosaccharide covalently joined to the
carboxyl-terminal residue of a protein through
phosphoethanolamine.

16
Glycosyl phosphatidylinositol (GPI)
anchors : phosphatidylinositol in which the
inositol bears a short oligosaccharide
covalently joined to the carboxyl-terminal
residue of a protein through
phosphoethanolamine.

FIGURE 11-14 Lipid-linked membrane proteins. Covalently

Attached lipids anchor membrane proteins to the lipid bilayer.

11.2 Membrane Dynamics

Acyl Groups in the Bilayer Interior Are Ordered to
Varying Degrees
‡ Although the lipid bilayer structure is quite stable, its
individual phospholipid and sterol molecules have
much freedom of motion (Fig. 11–15).
‡ The structure and flexibility of the lipid bilayer
depend on the kinds of lipids present, and change with
temperature. Below normal physiological
temperatures, the lipids in a bilayer form a semisolid
gel phase.

p.381

17
‡ In this liquid-disordered state, or fluid state (Fig. 11–
15b), the interior of the bilayer is more fluid than solid
and the bilayer is like a sea of constantly moving lipid.
At intermediate (physiological) temperatures, the
lipids exist in a liquid-ordered state.
Transbilayer Movement of Lipids Requires Catalysis
‡ At physiological temperatures, diffusion of a lipid
molecule from one leaflet of the bilayer to the other
(Fig. 11–16a) occurs very slowly if at all in most
membranes.

p.381

FIGURE 11-15

FIGURE 11–15 Two extreme states of bilayer lipids.

p.381

18
‡ Several families of proteins, including the flippases,
floppases, and scramblases.
‡ Flippases catalyze translocation of the
aminophospholipids phosphatidylethanolamine and
phosphatidylserine from the extracellular to the cytosolic
leaflet of the plasma membrane.
‡ Floppases move plasma membrane phospholipids from
the cytosolic to the extracellular leaflet.
‡ Scramblases are proteins that move any membrane
phospholipid across the bilayer down its concentration
gradient.
p.382

19
FIGURE 11-16

FIGURE 11–16
Motion of single
phospholipids in a
bilayer.
p.382

Study of Membrane Dynamics: FRAP

‡ Fluorescence Recovery After Photobleaching

(FRAP) allows to monitor lateral lipid diffusion by
monitoring the rate of fluorescence return
‡ From the rate of return of lipids, the diffusion
coefficient of a lipid in the leaflet can be determined
‡ Rates of lateral diffusion are high (up to 1 μm/sec):
„a lipid can circumnavigate E.coli cell in one
second

20
FIGURE 11-18 Hop diffusion of individual lipid molecules. The
motion of a single fluorescently labeled lipid molecule in a cell surface is recorded on video
by fluorescence microscopy, with a time resolution of 25 µs (equivalent to 40,000 frames/s).
The track shown here represents a molecule followed for 56 ms (2,250 frames); the trace
begins in the purple area and continues through blue, green, and orange. The pattern of
movement indicates rapid diffusion within a confined region (about 250 nm in diameter,
shown by a single color), with occasional hops into an adjoining region. This finding
suggests that the lipids are corralled by molecular fences that they occasionally jump

21
FIGURE 11-19 Restricted motion of the erythrocyte chloride-bicarbonate
exchanger and glycophorin. The proteins span the membrane and are tethered to
spectrin, a cytoskeletal protein, by another protein, ankyrin, limiting their lateral mobility.
Ankyrin is anchored in the membrane by a covalently bound palmitoyl side chain (see
Figure 11-14). Spectrin, a long, filamentous protein, is cross-linked at junctional
complexes containing actin. A network of cross-linked spectrin molecules attached to the
cytoplasmic face of the plasma membrane stabilizes the membrane, making it resistant to
deformation. This network of anchored membrane proteins may form the "corral"
suggested by the experiment shown in Figure 11-18; the lipid tracks shown here are
confined to regions defined by the tethered membrane proteins.

Membrane Rafts

‡ Lipid distribution in a single leaflet is not random and even

‡ Some regions contain clusters of glycosphingolipids with
longer than usual tails
‡ These regions are more ordered and contain specific
doubly- or triply-acylated proteins
‡ Rafts allow segregation of proteins in the membrane

22
FIGURE 11-20a Membrane microdomains (rafts). (a) Stable
associations of sphingolipids and cholesterol in the outer leaflet produce a
microdomain, slightly thicker than other membrane regions, that is enriched with
specific types of membrane proteins. GPI-linked proteins are common in the outer
leaflet of these rafts, and proteins with one or several covalently attached long-
chain acyl groups are common in the inner leaflet. Caveolin is especially common
in inwardly curved rafts called caveolae (see Figure 11-21).

FIGURE 11-20b Membrane microdomains (rafts). (b) In this artificial

membrane—reconstituted (on a mica surface) from cholesterol,
synthetic phospholipid (dioleoylphosphatidylcholine), and the GPI-
linked protein placental alkaline phosphatase—the greater thickness of
raft regions is visualized by atomic force microscopy (see Box 11-1).
The rafts protrude from a lipid bilayer ocean (the black surface is the
top of the upper monolayer); sharp peaks represent GPI-linked
proteins. Note that these peaks are found almost exclusively in the rafts.

23
FIGURE 11-21 Caveolin forces inward
curvature of a membrane. Caveolae are
small invaginations in the plasma
membrane, as seen in (a) an electron
micrograph of an adipocyte surface-
labeled with an electron-dense marker. (b)
Each caveolin monomer has a central
hydrophobic domain and three long-chain
acyl groups (red), which hold the molecule
to the inside of the plasma membrane.
When several caveolin dimers are
concentrated in a small region (a raft), they
force a curvature in the lipid bilayer,
forming a caveola. Cholesterol molecules in
the bilayer are shown in orange.

Membrane Fusion

‡ Membranes can fuse with each other without

losing continuity
‡ Fusion can be spontaneous or protein-mediated
‡ Examples of protein-mediated fusion are
„Entry of influenza virus into the host cell
„Release of neurotransmitters at nerve synapses

24
 FIGURE 11-22

FIGURE 11–22
Membrane fusion. The
fusion of two membranes
is central to a variety of
cellular processes
involving organelles and
the plasma membrane.

p.387

FIGURE 11-23 Three

models for protein-induced
curvature of membranes.

p.387

25
 Neurotransmitter Release

Integral Proteins of the Plasma Membrane Are

Involved in Surface Adhesion, Signaling, and Other
Cellular Processes
‡ Integrins are surface adhesion proteins that mediate a cell’s
interaction with the extracellular matrix and with other cells.
‡ Other plasma membrane proteins involved in surface
adhesion are the cadherins, which undergo homophilic
interactions with identical cadherins in an adjacent cell.
Selectins have extracellular domains that, in the presence of
Ca2+, bind specific polysaccharides on the surface of an
adjacent cell.

p.388

26
 11.3 Solute Transport across Membranes

‡ Some solutes passively diffuse through the lipid

membrane
‡ Passive diffusion of polar molecules involves
desolvation and thus has a high activation barrier
‡ Transport across the membrane can be facilitated
by proteins that provide an alternative diffusion
path
‡ Such proteins are called transporters or permeases

FIGURE 11-25

FIGURE 11–25 Summary of transport types. p.389

27
 Passive Transport Is Facilitated by Membrane Proteins
‡ When two aqueous compartments containing unequal
concentrations of a soluble compound or ion are separated
by a permeable divider (membrane), the solute moves by
simple diffusion from the region of higher concentration,
through the membrane, to the region of lower
concentration, until the two compartments have equal
solute concentrations (Fig. 11–26a).

p.390

Electrochemical gradient
Chemical gradient Electrical gradient

FIGURE 11-26 Movement of solutes across a permeable membrane.

(a) Net movement of an electrically neutral solute is toward the side of lower
solute concentration until equilibrium is achieved. The solute concentrations on
the left and right sides of the membrane are designated C1 and C2. The rate of
transmembrane movement (indicated by the arrows) is proportional to the
concentration gradient, C2/C1.
(b) Net movement of an electrically charged solute is dictated by a combination
of the electrical potential (Vm) and the chemical concentration difference (C2/C1)
across the membrane; net ion movement continues until this electrochemical
potential reaches zero. p.390

28
Passive Transport Is Facilitated by Membrane Proteins
‡ The direction of a charged solute tends to move across a
membrane depends on:
(1) chemical gradient (the difference in solute concentration)
(2) electrical gradient across the membrane (called
electrochemical gradient or electrochemical potential)
‡ Membrane proteins lower the activation energy for transport of
polar compounds and ions by providing an alternative path
through the bilayer for specific solutes. Proteins that bring
about this facilitated diffusion, or passive transport, are not
enzymes in the usual sense
‡ Membrane proteins that speed the movement of a solute across
a membrane by facilitating diffusion are called transporters or
permeases.

FIGURE 11-27

FIGURE 11–27 Energy

changes accompanying
passage of a hydrophilic
solute through the lipid
bilayer of a biological
membrane.

p.390

29
 Transporters Can Be Grouped into Superfamilies Based
on
Their Structures
‡ Transporters fall within two very broad categories:
carriers and channels (Fig. 11–28). Carriers bind their
substrates with high stereospecificity, catalyze transport
at rates well below the limits of free diffusion, and are
saturable in the same sense as are enzymes.
‡ Channels generally allow transmembrane movement at
rates orders of magnitude greater than those typical of
carriers, rates approaching the limit of unhindered
diffusion.

p.391

FIGURE 11-28

FIGURE 11–28 Classification of transporters.

p.391

30
 Proposed structure of
GLUT1

Hydrophilic
surface

multimeric
Hydrophobic structure
surface

Glucose Transporter of Erythrocyte mediates passive

transport
Glucose transporter (Glu T1, in erythrocytes)
(1)12 transmembrane domain proposed
(2)From helical wheel disgram, we can see each helical
segment contains two surfaces: hydrophobic and
hydrophilic surfaces.
(3)Proposed three dimensional structure:
5 or 6 helical segments arranged as a channel-like
structure
hydrophilic surface toward inward and can form H-bond
with solute glucose

31
 Analogous to Michaelis-Menten Equation:

V [S] 1 = Kt 1 + 1
V0 = Kmax+[ S ]
out
V0 Vmax [ S ] Vmax
t out
k2 + k−1
(Kt = k1
; whenk 2 << k−1
k
K t = k−1 = dissociationconst )
1

V [S]
V0 = Kmax+[ S ]
out
For Glu T1, t out
Kt = 1.5mM for D-Glucose
Kt = 20 – 30 mM for D-Mannose & D-Galactose
Kt = 1.5mM for L-Glucose
GluT1: (1) high rates for diffusion down a conc. gradient
(2) specificity (3) saturability
[Blood sugar]= 4.5 to 5mM, about 3x Kt, therefore V0~Vmax

32
The Chloride-Bicarbonate Exchanger Catalyzes Electroneutral
Cotransport of Anions across the Plasma Membrane

‡ The chloride-bicarbonate exchanger (cotransport system),

also called the anion exchange (AE) protein, increases the
rate of HCO3– transport across the erythrocyte membrane more
than a millionfold. Entry and exit of HCO3– and Cl- without
changes in the transmembrane electrical potential (called
“electroneutral”).

‡ When two substrates move in opposite directions, the process

is antiport. In symport, two substrates are moved
simultaneously in the same direction. Transporters that carry
only one substrate, are known as uniport systems.

p.393

33
1 FIGURE 11-32 2

HCO3- is more soluble

in blood than CO2

HCO3- then
transferred to lung

FIGURE 11–32
Chloride-
4 3 bicarbonate
exchanger of the
erythrocyte
membrane. p.395

FIGURE 11–33

FIGURE 11–33 Three general classes of transport systems.

p.395

34
 Active Transport Results in Solute Movement against a
Concentration or Electrochemical Gradient
‡ In primary active transport, solute accumulation is
coupled directly to an exergonic chemical reaction, such
as conversion of ATP to ADP + Pi (Fig. 11–34).
‡ Secondary active transport occurs when endergonic
(uphill) transport of one solute is coupled to the exergonic
(downhill) flow of a different solute that was originally
pumped uphill by primary active transport.

p.395

FIGURE 11–34

FIGURE 11–34 Two types of active transport. (a) In primary active

transport, the energy released by ATP hydrolysis drives solute movement
against an electrochemical gradient. (b) In secondary active transport, a
gradient of ion X (often Na+) has been established by primary active transport.
Movement of X down its electrochemical gradient now provides the energy
to drive cotransport of a second solute (S) against its electrochemical gradient.
p.395

35
Active Transport Results in Solute Movement against a
Concentration or Electrochemical Gradient

Recall ΔG = ΔG o + RT ln Q ; Q: reaction quoient

[ S ]in [ S ]out
For a transport system: Q= or =
[ S ]out [ S ]in
Q ΔG o = 0 (since no bonds are made or broken)
Free energy change for transport:
C
∴ ΔGt = RT ln 2
C1
Ex. 11-1: pumping uncharged solute against 10-4 fold
C
ΔGt = RT ln 2 = 8.314* 298* ln(1.0 *10−4 )
C1
= 23 kJ/mol

(I) For a transport process of ‘uncharged’ solutes:

C
∴ ΔGt = RT ln 2
C1
(II) For a transport process of ‘charged’ solutes:
C
∴ ΔGt = RT ln 2 + zFΔψ
C1
Z : charge of the solute
Δψ : transmembrane electrical potential (in volts)
(for eukaryotic cells, Δψ = 50 to 200mV

Ex. 11-2: pumping charged solute

C −3
ΔGt = RT ln 2 + zFΔψ = 8.314 * 310 * ln(1.0*10−7 )
C1 1.0*10
+ 2 * 96500 * 0.050 = 33kJ/mol

36
There are at least four general types of transport ATPase

(I)P-Type ATPases Undergo Phosphorylation during Their

Catalytic Cycles
‡ The family of active transporters called P-type ATPases are
cation transporters that are reversibly phosphorylated by
ATP as part of the transport cycle.
‡ In animal tissue, the Na+K+ATPase and Ca2+ ATPase are
ubiquitous P-type ATPase that maintain the differences in the
ionic composition of cytosol and extracellular medium.
‡ The plasma membrane Ca2+ pump moves calcium ions out
of the cell, and another P-type pump in the endoplasmic
reticulum moves Ca2+ into the ER lumen.

p.396

‡ The sarcoplasmic and endoplasmic reticulum calcium

(SERCA) pumps are P-type ATPases closely related in
structure and mechanism.
‡ A variation on this basic mechanism is seen in the Na+K+
ATPase of the plasma membrane, discovered by Jens
Skou in 1957.

p.397

37
ATP-drive Ca2+ pumps (P-type ATPase) maintain
a low conc. of calcium in the cytosol
‡ Calcium ions are pumped out of the cytosol by plasma
membrane Ca2+ pump (a P-type ATPase) to maintain low
conc. of calcium in the cytosol
‡ Calcium ions are pumped in to the lumen by endoplasmic
reticulum and sacoplasmic reticulum Ca2+ pump
(SERCA).
‡ SERCA conatins a single polypeptide (Mr ~ 100,000) and
cycles among conformations (see Fig. 11-36).
‡ Two calcium ions bind to a transmembrane domain.
Phosphorylation on Asp351 mediates conformational
change and controls calcium release/binding.
p.396

FIGURE 11–35 The Ca2+ pump of sarcoplasmic reticulum: a SERCA

pump.
p.397

38
 FIGURE 11–36

FIGURE 11–36 Postulated mechanism of the SERCA pump.

p.398

A P-Type ATPases catalyzes active cotransport of Na+ and K+

FIGURE 11–38
Jens Skou discovered Na+K+
ATPase of the plasma
membrane in 1957.
‡ 1 molecule of ATP
hydrolyzed to ADP,
‡ 2 molecules of K+ move
inwards
‡ 3 Na+ move outwards.

FIGURE 11–38 Role of

the Na+K+ ATPase in
animal cells.
p.398

39
(1)ATP + EnzI ADP + P-EnzII
FIGURE
(2)P-EnzII + H11–37
2O EnzI + Pi

Net:
ATP+ H2O ADP +Pi

(1)EnzI binds 3 Na+

(2) P-EnzII binds to 2 K+
“cycle between two conformations”
(3)Ouabian (箭毒 , a steroid derivative)
can bind to Na+K+ ATPase and prevent
the conformational change, then
prohibit the cycling Na+K+ ATPase
FIGURE 11–37 Postulated
mechanism of the Na+K+
ATPase. p.398

F-Type ATPases Are Reversible, ATP-Driven Proton

Pumps
‡ F-type ATPase active transporters catalyze the uphill
transmembrane passage of protons driven by ATP
hydrolysis.
‡ V-type ATPases, a class of proton-transporting ATPases
structurally (and possibly mechanistically) related to the
F-type ATPases, are responsible for acidifying
intracellular compartments in many organisms.

p.399

40
FIGURE 11–39

FIGURE 11–39 F0F1 ATPase/ATP synthase.

p.399

41
ABC Transporters Use ATP to Drive the Active
Transport of a Wide Variety of Substrates
‡ ABC transporters (Fig. 11–41) constitute a large family
of ATP-dependent transporters that pump amino acids,
peptides, proteins, metal ions, various lipids, bile salts,
and many hydrophobic compounds.
‡ One ABC transporter in humans, the multi-drug
transporter (MDR1), is responsible for the striking
resistance of certain tumors to some generally effective
antitumor drugs.

p.400

FIGURE 11–41

FIGURE 11–41 An ABC transporter of E. coli.

p.400

42
 TABLE 11-4

p.400

Ion Gradients Provide the Energy for Secondary

Active Transport
+
‡ Ion gradient: e.g. Na K+ ATPase 使得
細胞內 [Na+] <細胞外 [Na+] (against the gradient,
therefore need the hydrolysis of ATP to provide energy)
‡ Cell 利用此ion gradient由另外一個transporter 將Na+
and其他solutes symport 進入cytosol (因為是down the
gradient, 所以不需利用ATP hydrolysis 以提供能量),此
種系統稱為secondary active transport
‡ see more examples in table 11-4

‡ Na+K+ ATPase and some other H+ pump 主要功能為提

供 ion gradient for other transporter 攜帶其他solutes 進
出cells.
p.402

43
Ion Gradients Provide the Energy for Secondary
Active Transport
‡ 例子一: The lactose transporter (lactose permease)
of E. coli is the well-studied prototype for proton-
driven cotransporters.
‡ 例子二: Na+-glucose symporters in the apical
plasma membrane take up glucose from the
intestine in a process driven by the downhill flow
of Na+:
2Naout+ + glucoseout → 2Nain+ + glucosein

FIGURE 11–42

FIGURE 11–42 Lactose uptake in E. coli. (a) The primary transport of H+ out of
the cell, driven by the oxidation of a variety of fuels, establishes both a proton
gradient and an electrical potential (inside negative) across the membrane.
Secondary active transport of lactose into the cell involves symport of H+ and
lactose by the lactose transporter. The uptake of lactose against its concentration
gradient is entirely dependent on this inflow of protons driven by the
electrochemical gradient. p.402

44
FIGURE 11–44

FIGURE 11–44 Glucose transport in intestinal epithelial cells.

p.403

45
Ion-Selective Channels Allow Rapid Movement of Ions
across
Membranes
‡ Ion-selective channels Ion channels, together with ion
+ +
pumps such as the Na K ATPase, determine a plasma
membrane’s permeability to specific ions and regulate the
cytosolic concentration of ions and the membrane
potential.
‡ In ligand-gated channels binding of an extracellular or
intracellular small molecule forces an allosteric transition
in the protein.
p.406

‡ Voltage-gated ion channels, a change in transmembrane

electrical potential (Vm) causes a charged protein domain
to move relative to the membrane.
‡ Ion channels provide hydrophilic pores through which
select ions can diffuse, moving down their electrical or
chemical concentration gradients; they characteristically
are unsaturable, have very high flux rates, and are highly
specific for one ion.

p.406

46