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    Exp 4 - F23

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    s127886
    This experiment determines the vitamin C content of commercial tablets. Vitamin C (ascorbic acid) is oxidized by iodine in an acid solution. The method involves generating excess iodine from a reaction between iodide and iodate, then back-titrating the excess iodine with sodium thiosulfate. A tablet is dissolved and reacted with iodide and iodate, then titrated with thiosulfate. The amount of thiosulfate used indicates the iodine consumed by reaction with vitamin C, allowing calculation of the vitamin C content. Starch solution is added at the pale yellow endpoint of the titration, forming a dark complex to indicate the reaction is

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    Exp 4 - F23

    Uploaded by

    s127886
    12 views2 pages
    This experiment determines the vitamin C content of commercial tablets. Vitamin C (ascorbic acid) is oxidized by iodine in an acid solution. The method involves generating excess iodine from a reaction between iodide and iodate, then back-titrating the excess iodine with sodium thiosulfate. A tablet is dissolved and reacted with iodide and iodate, then titrated with thiosulfate. The amount of thiosulfate used indicates the iodine consumed by reaction with vitamin C, allowing calculation of the vitamin C content. Starch solution is added at the pale yellow endpoint of the titration, forming a dark complex to indicate the reaction is

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    EXP 4_F23

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    This experiment determines the vitamin C content of commercial tablets. Vitamin C (ascorbic acid) is oxidized by iodine in an acid solution. The method involves generating excess iodine from a reaction between iodide and iodate, then back-titrating the excess iodine with sodium thiosulfate. A tablet is dissolved and reacted with iodide and iodate, then titrated with thiosulfate. The amount of thiosulfate used indicates the iodine consumed by reaction with vitamin C, allowing calculation of the vitamin C content. Starch solution is added at the pale yellow endpoint of the titration, forming a dark complex to indicate the reaction is

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    12 views2 pages

    Exp 4 - F23

    Uploaded by

    s127886
    This experiment determines the vitamin C content of commercial tablets. Vitamin C (ascorbic acid) is oxidized by iodine in an acid solution. The method involves generating excess iodine from a reaction between iodide and iodate, then back-titrating the excess iodine with sodium thiosulfate. A tablet is dissolved and reacted with iodide and iodate, then titrated with thiosulfate. The amount of thiosulfate used indicates the iodine consumed by reaction with vitamin C, allowing calculation of the vitamin C content. Starch solution is added at the pale yellow endpoint of the titration, forming a dark complex to indicate the reaction is

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    CHEM 4441 - ANALYTICAL CHEMISTRY II

    EXPERIMENT 4: DETERMINATION OF ASCORBIC ACID IN VITAMIN C TABLETS

    Introduction

    In this experiment, you are required to determine the vitamin C content of a commercial tablet and compare
    this with the manufacturer's specification if any! Vitamin C, ascorbic acid, is rapidly and quantitatively
    oxidized by iodine in acidic solutions according to the following equation:

    The standard method for determination of ascorbic acid involves the direct titration of acidified samples
    with a standard iodine solution. But the low solubility of iodine makes this procedure less than ideal.The
    proposed experiment avoids these difficulties by using the reaction between iodide (in excess) and iodate
    which generate a known excess quantity of iodine, and this excess iodine is back titrated with standard
    sodium thiosulfate solution.
    The reactions are as follows:
    IO3-(aq) + 5I-(aq) + 6H+(aq)  3I2(aq) + 3H2O(l)
    I2(aq) + 2S2O32-(aq)  2I-(aq) + S4O62-(aq)

    Experimental
    Chemicals:
     Vitamin C tablet, (1)
     standard 0.0110 M potassium iodate (KIO3) solution, (100 cm3)
     standard 0.060 M sodium thiosulphate solution, (Na2S2O3)
     1 M potassium iodide solution, (KI)
     0.5 M H2SO4,
     Freshly prepared starch solution (1%).

    1
    Procedure:
    a. Dissolve the vitamin-C tablet provided in about 75 cm3 of 1 M sulfuric acid.
    b. Transfer the resulting solution to a clean volumetric flask and make up to 250 cm3 using distilled water.
    c. Pipette 10.0 cm3 of vitamin C solution into a conical flask and add to it 2 cm3 of 1 M potassium iodide
    solution. Then pipette 10.0 cm3 of the standard KIO3 solution into the flask containing vitamin C and
    potassium iodide. The excess iodine is immediately back titrated with the standard sodium thiosulphate
    solution. Add a few drops of freshly prepared starch solution when the reaction mixture turns pale
    yellow and continue to titrate to the end point slowly.
    Record your results into the following table:

    Titration table
    Titration Trial 1 2 3 4
    Final burette reading (cm3)
    Initial burette reading (cm3)
    Volume of Na2S2O3 added (cm3)

    1. From the results of the titration table, find the number of moles of excess I2 in the solution.
    2. Find also the number of moles of iodine produced by the standard KIO3 solution added.
    3. Calculate number of moles of iodine that has reacted with vitamin C and hence the mass of ascorbic acid
    per tablet. (Repeat the calculations for each trial separately to help you perform statistical analysis of
    the results)
    4. What is the function of the starch solution? Why it has to be added only when the reaction mixture
    becomes pale yellow?

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