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Restriction Mapping Problems

This document provides instructions for two restriction mapping problems in AP Biology. For the first problem, students are given size data from digesting a linear DNA fragment with various restriction enzymes alone or in combination, and are asked to construct a restriction map indicating the positions and distances of restriction sites. For the second problem, students are given size data from digesting a circular plasmid with various restriction enzymes alone or in combination, and are again asked to construct a restriction map indicating the positions and distances between restriction sites.

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333 views

Restriction Mapping Problems

This document provides instructions for two restriction mapping problems in AP Biology. For the first problem, students are given size data from digesting a linear DNA fragment with various restriction enzymes alone or in combination, and are asked to construct a restriction map indicating the positions and distances of restriction sites. For the second problem, students are given size data from digesting a circular plasmid with various restriction enzymes alone or in combination, and are again asked to construct a restriction map indicating the positions and distances between restriction sites.

Uploaded by

yashasvi.art
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Restriction Mapping Problems – AP Biology

1. Construct a restriction map of a linear fragment of DNA, using the following data. Your
map should indicate the relative positions of the restriction sites along with distances
from the ends of the molecule to the restriction sites and between restriction sites:

DNA Sizes of Fragments (bp)


1. uncut DNA
2. DNA cut with EcoRI
3. DNA cut with HindIII
4. DNA cut with BamHI
5. DNA cut with EcoRI + HindIII
6. DNA cut with EcoRI + BamHI
7. DNA cut with HindIII + BamHI

1 2 3 4 5 6 7
2. Construct a restriction map of a circular DNA plasmid, using the following data. Your map
should indicate the relative positions of the restriction sites along with distances between
restriction sites:

Sizes of Fragments
DNA
(bp)
uncut DNA 7950
DNA cut with BglII 7950
DNA cut with EcoRI 7950
DNA cut with HpaI 7950
DNA cut with BglII + EcoRI 5416, 2534
DNA cut with BglII + HpaI 6632, 1318
DNA cut with EcoRI + HpaI 4098, 3852
Answer Sheet – Restriction Mapping Name__________________________________

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