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    LifeTein Peptide Synthesis Service Overview

    Uploaded by

    James Chou
    LifeTein®, located in South Plainfield, New Jersey, was founded in 2008 with a simple vision: to increase both the quality and understanding of life one protein at a time. This philosophy has formed the foundation of every peptide, antibody, protein, antibody, and chemical project that we have completed for our customers in the academic, biotechnology, agricultural, government institutions and pharmaceutical fields. We offer a wide range of custom research services and a staff of scientists with the first-hand campus-core-facility experience necessary to understanding the role of peptide and protein services in large research projects. Visit us at: www.lifetein.com

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    LifeTein Peptide Synthesis Service Overview

    Uploaded by

    James Chou
    115 views20 pages
    LifeTein®, located in South Plainfield, New Jersey, was founded in 2008 with a simple vision: to increase both the quality and understanding of life one protein at a time. This philosophy has formed the foundation of every peptide, antibody, protein, antibody, and chemical project that we have completed for our customers in the academic, biotechnology, agricultural, government institutions and pharmaceutical fields. We offer a wide range of custom research services and a staff of scientists with the first-hand campus-core-facility experience necessary to understanding the role of peptide and protein services in large research projects. Visit us at: www.lifetein.com

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    LifeTein®, located in South Plainfield, New Jersey, was founded in 2008 with a simple vision: to increase both the quality and understanding of life one protein at a time. This philosophy has formed the foundation of every peptide, antibody, protein, antibody, and chemical project that we have completed for our customers in the academic, biotechnology, agricultural, government institutions and pharmaceutical fields. We offer a wide range of custom research services and a staff of scientists with the first-hand campus-core-facility experience necessary to understanding the role of peptide and protein services in large research projects. Visit us at: www.lifetein.com

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    115 views20 pages

    LifeTein Peptide Synthesis Service Overview

    Uploaded by

    James Chou
    LifeTein®, located in South Plainfield, New Jersey, was founded in 2008 with a simple vision: to increase both the quality and understanding of life one protein at a time. This philosophy has formed the foundation of every peptide, antibody, protein, antibody, and chemical project that we have completed for our customers in the academic, biotechnology, agricultural, government institutions and pharmaceutical fields. We offer a wide range of custom research services and a staff of scientists with the first-hand campus-core-facility experience necessary to understanding the role of peptide and protein services in large research projects. Visit us at: www.lifetein.com

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 20

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    Outline

    Market & Technology Trends LifeTein Technology Portfolio LifeTein Services

    Synthetic Therapeutic Peptides

    More than 60 synthetic therapeutic peptides under 50 amino acids in size have reached the pharmaceutical market as APIs or generics.

    Chemical Optimization

    Drug Discovery Today Volume 15, Numbers 1/2 January 2010

    Strategies for Lead Peptide Optimization

    Technology Trends

    Microwave Synthesis

    Using Nitroalkanes as Acyl Anion Equivalents


    www.nature.com/doifinder/10.1038/nature09125

    Green Chemistry for Synthesis


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    LifeTein Technology Portfolio

    PeptideSynTM Platform Provides high-yield peptide synthesis Combines SPPS and LPPS for the highest synthesis success rate possible Ensures shorter turnaround

    LifeTein Technology Portfolio

    PeptideSynTM Ligation Synthesizes long peptides (up to 120 amino acids) Ensures high yield and short turnaround

    LifeTein Technology Portfolio

    A: H-MEDSTYYKASKGC-NH2 B: H-MEDST(PTyr)YKASKGC-NH2 C: H-PKYLQNTLKLATGMRNVPEKQTT-OH D: H-MEDSTYYKASKGC-NH2 E: H-MEDST(PTyr)YKASKGC-NH2 F: H-PKYLQNTLKLATGMRNVPEKQTT-OH

    LifeTein Services

    Custom Peptide Synthesis Service Custom Peptide Library Synthesis Service Custom Polyclonal Antibody Service Custom Monoclonal Antibody Service

    LifeTein Services
    Advantages of PeptideSynTM Technology
    Increases yields and reduces costs Shortens production cycle Combines the advantages of both solid and solution phase methods Can scale up projects rapidly to over 100 kg Avoids solubility problems for long segments in solution phase

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    Peptide Synthesis Service


    Peptide Length Peptide Scale Peptide Purity Peptide Modifications Peptide Labels Carrier Proteins Cyclic Peptides Analysis Peptide Libraries Others 5-120 amino acids From mg to kg Crude, desalted, >75%, >85%, >90%, >95%, 98%, and >99% 2-5 phosphorylation sites, methylation, acetylation, amidation, MAPs(2, 4 or 8 chains), more FITC, Biotin, 5-FAM, Rhodamine, Dansyl, TAMRA, more KLH, BSA, OVA Amide cyclic peptide (head to tail, side chain), 1, 2, or 3 disulfide bond bridges HPLC, MS, Amino Acid Analysis (AAA), solubility testing, microbial analysis, more Peptide sequence length of 5-20 amino acids, 48, 96 peptides Free aliquots, spacers and linkers (Ahx, mini-PEG), more

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    Free Aliquot Service

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    Case Studies
    Cell Penetrating Peptides
    Name
    Tat family Tat (48-60) Oligoarginine Penetralia family p-Antp Antermapedia homeodomain RQIKIWFQNRRMKWKK HIV-1 protein Tat derivative GRKKRRQRRRPPQQ Rn

    Origin

    Sequence

    plsl
    Chimeric CPPs Transportan

    Igl-1 homeodomain

    RVIRVWFQNKRCKDKK

    Galanin-mastoparan

    GWTLNSAGYLLGKINLKALAA LAKKIL

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    Case Studies
    Cell Penetrating Peptides

    Attached to a CPP, therapeutic cargo is delivered to an intracellular target, thus overcoming the entry restrictions set by the plasma membrane.

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    Case Studies
    N-Terminal Acetylation and C-Terminal Amidation
    Both modifications are free of charge

    Chemically synthesized peptides have free amino and carboxyl termini.

    N-terminal acetylation and C-terminal amidation reduce the overall charge of the peptide. This can increase the stability of the peptide and its resemblance to the native protein.

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    Peptide Antigen Design

    Continuous versus Discontinuous Epitopes?

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    Peptide Antigen Design


    Sequence Length Peptide Purity Peptide Solubility 8-20 amino acids

    >75% for antibody generation, >90% for phosphorylated peptide antibody (recommended)
    Keep the hydrophobic amino acid content below 50% At least one charged residue for every five amino acids

    Difficult Amino Acids

    Cys and Met can undergo rapid oxidation Avoid multiple Ser or Pro Avoid multiple or adjacent -sheet forming amino acids: Glu, Iso, Leu, Phe, Thr, Tyr, Val KLH, BSA, OVA

    Carrier Proteins

    Multiple Antigenic Peptides (MAPs) 17

    Custom Antibody Service


    Polyclonal Antibodies Monoclonal Antibodies

    Peptide Antigen Antibody Services

    Phosphorylated-Peptide Antibody Services

    Polyclonal Antibodies Monoclonal Antibodies

    Methylated-Peptide Antibody Services

    Polyclonal Antibodies Monoclonal Antibodies

    AdjuBoosterTM Adjuvant Technology 18

    More information, please visit www.lifetein.com

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    Contact Information

    LifeTein LLC 150 Maple Ave South Plainfield, NJ 07080 USA

    info@lifetein.com 1-732-312-5852 1-800-655-5405 (Toll Free) 1-732-626-6074 (Fax)

    Let us be a partner to your growth!


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