GEA Service Manual V1.4e
GEA Service Manual V1.4e
GEA Service Manual V1.4e
Microplate Reader
Service Manual
1
Nota para el uso de este manual
Nota: para errores incorregibles, o errores que se repiten concurrentemente durante el uso por
favor, contactar con el distribuidor.
Este manual es objeto de cambio sin necesidad de notificación previa. LiNEAR Chemaicals S.L.,
puede mejorar y/o modificar los productos, al igual que el programa que se detalla en
este manual si notificación previa.
2
Descripción General
1.1. Introducción
1) GEA is a microprocessor –controlled, general purpose photometer system designed to read and calculate
the result of assays, including contagion, tumorous mark, hemopathy, dyshormonism, which are read in
microplate.
2) Touch panel and pen brings you convenience in operation.
3) 100 tests can be programmed.
4) Multiple calculations:
Absorbance mode (ABS)
Cut-Off mode
Single standard mode
Point to point mode
% Absorbance Multi-Point Mode
Linear regression mode
Exponent regression mode
Logarithm regression mode
Power regression mode
5) Visual allocation of microplate,you can set blank, control, sample ,standard in any place,and perform 12
different tests in one 96 microplate.
6) Test time <5S/plate,and mix plate before test
7) Maximum 1000 patient data and 10000 sample records can be saved.
8) Comprehensive report ,support common printers.
9) Information manage function:Department database, operator database, system log database.
3
1.2. Technical parameters
Weight: 7.5kg
Overall dimensions: 460mm(L)×330mm(W)×190mm(H)
Power: a.c.110V~220V,50/60Hz
Fuses: T3.15AL250V
Work Environment: temperature 10℃~40℃;Humidity ≤85%
Store Environment: -10℃~40℃;Humidity ≤85%
Lamp: Halogen tungsten lamp
Standard Wavelength: 405,450,492,630nm,(substitute filters from 340-700nm
available on special order)
Accuracy: 0-2.0A:±1.0% or ±0.007A
Precision: 0-2.0A:±0.5% or ±0.005A
Linearity: ±2.0% or±0.007A
Reading speed: Continuous mode <5s,step by step mode<15s
Warm up time: 1 minute
Interface: RS-232C serial interface, Interface for USB, SD card and LAN
Display: 5.7″LCD display(320×240 discernibility,256 gray scale)
Input: Touch panel and pen, external mouse(Optional)
4
1.3. Diagram of function
5
1.4. Function description of each part
6
1.5. Optic system
Optical diode
Filter wheel
Reference path Heat absorb board and lens
Membran
Fiber
e
board
Lamp
③
②
⑦ ④ ⑤ ⑥
7
①
② ③ ⑧
LINE PRINTER RS232
PO WER ACINPUT
ON/OFF
①②③ ④ ⑤ ⑥
① Power switch
② Power socket
③ Fuses
④ Interface for SD Card
⑤ Interface for USB
⑥ Interface for LAN
⑦ RS-232 interface
⑧ Brightness adjuster (to adjust the brightness of displaying)
8
2. Installation and primary operation
Unseal the packing of the instrument and remove the material used for conveyance. Retain the packing
box and packing material for the purpose of re-packing the instrument in future.
1) Take the instrument out of the box.
2) Remove the packing material and take the instrument out of the plastic packing bag.
3) Check the contents of the packing box for the following:
GEA main system
user manual
Packing list
guarantee card
Accessories: power line, RS-232 serial cable, touch pen and spare fuses.
Note: if any part is found missing or damaged or not conform to the encasement checklist,
please contact the dealer.
Find a location in you working place that is not directly exposed to the sun. The working table selected
shall have a smooth surface as well as an enough space to put the GEA. The front edge of the instrument shall
be close to the edge of the working table. Avoid much vibration on the working table (e.g. a centrifugal
machine is put on the table).
Note: the ambient temperature of the instrument shall be 10℃-40℃, and the humidity shall
be≤85%.
In order to ensure the normal working of the instrument, it must not be put in the following places:
Where there is a wide temperature range;
Where it is too hot or too cold;
Where there is a lot of dust;
Close to the electromagnetic equipment that generates magnetic field;
AC110V - AC220V
50 / 60Hz
120VA
9
Note:
AC mains must have a good earthing (zero-ground voltage<5V=.
AC power supply must be stable and the instrument must not share the AC mains with
large-power electrical appliances.
Must clutch at the plug itself, not at the power cord, to disconnect the power cord.
If fumes, peculiar smells or strange sounds are found given forth from the instrument, please cut off the
electricity supply immediately and contact the dealer.
1) Make sure that the printer and instrument have been turned off.
2) Connect one end of the USB printer cable to the USB interface of the printer.
3) Connect the other end of the USB printer cable to the USB Interface of instrument.
4) Connect the printer to power supply by the power line for the printer.
Note:To make the printer work properly, you must turn on the instrument before you turn on
the printer.
When you are inserting or pulling out the printer cable, you must make sure that the
printer has been turned off. Or it may cause damage to the instrument and make it
work abnormally.
10
3. Daily maintenance and spare parts replacement
GEA is a precision instrument for clinic analysis, and in order to keep the instrument in a good condition,
daily maintenance is a must. The maintenance of GEA is very simple, but one must do it with care.
Note: please keep this instrument away from any organic solvent, grease or corrosive
substance.
Steps of disassemble
Steps of disassembly:
1) Fix the plate cover of the microplate reader with adhesive paper.
2) Switch off the instrument and the printer and disconnect the power cord.
3) Remove the printer cable.
4) Remove the screws used to fix the top cover with a cross screwdriver, as shown in figure 3-1.
5) Lift the top cover carefully and you can see the inside of the instrument.
6) Remove the corresponding parts according to your requirement.
Figure 3-1
11
Figure 3-2
Note: you must observe carefully the positions and wiring of the original parts before replacing
them, so as to restore the instrument.
Fuse box
Figure 3-3
Figure 3-4
2、After taking out the fuse socket, as shown in figure 3-5, take the fuse out form the socket and replace a
12
new one. Install the fuse socket into the fuse box again. Use a screwdriver to push the fuse socket in
and turn it clockwise.
Fuse
Fuse socket
Figure 3-5
3.3.2. Replacement of power switch
1、 Switch off the instrument, remove the screw which fixed the top cover and remove the cover
Figure 3-6
2、 Press out the spring piece ④ at the two sides of switch socket, take out the whole switch socket,
the structure of power switch is shown as figure 3-6
3、 Take off the wires from the switch, change a new switch and connect the wires as original, then
re-install the switch
Note:Please pay attention to the original position of wires, they must be correctly connected.
You’d better connect the wire to new switch as soon as you take it form the old switch.
Note: You must wait for the lamp to cool down, then you can replace it.
1、 Lift the top cover of instrument, you can see the lamp, as shown in figure 3-7, the lamp is shown as
figure 3-8.
13
Lamp
Lamp socket
Figure 3-7
Figure 3-8
2、 As shown in figure 3-9, remove the screw and spring piece which fix the lamp, press the lamp down
slightly, take off the lamp form lamp socket, disconnect the wires on lamp, replace a new lam and
connect the wires again.
3、 The installation of lamp is shown in figure 3-10, press down the lamp slightly, push it into the lamp
socket and make it locked, then fix the lamp by screw and spring piece as original. At last you shall
check if the lamp has been fixed tightly. If not, you must take off the lamp again; adjust the spring
piece to make sure that it has enough power to fix the lamp.
Spring piece
Figure 3-9
14
Figure 3-10
3.3.4. Replacement of plate carrier
1、Lift the top cover of instrument, remove the black shield box on the front board.
2、Remove the fixing bracket at right side of plate carrier, take off the two metal rails.
Fixing bracket
Figure 3-11
Plate carrier
Metal rail
Figure 3-12
3、As shown in figure 3-12, loosen the belt on the plate carrier, take it off from the motor at left side of
plate carrier, then upturn the plate carrier.
15
Figure 3-12
4、Remove the two screws, you can take off the plate carrier. Replace a new one, re-install it as original, fix
the screws.
Gear
Figure 3-14
5、Upturn the plate carrier, install the belt to the gear on the plate carrier motor at left side, as shown in
figure 3-14.
6、Install the metal rails and fixing bracket, adjust the belt to make the plate carrier moves smoothly,
install the shield box and top cover of instrument.
3.3.5. Replacement of the belt.
1、Lift the top cover of instrument, remove the shield box on front board.
2、Remove the fixing bracket at right side of plate carrier, take out the two metal rails.
Plate carrier
Metal rail
Fixing bracket
(a) (b)
Figure 3-15
16
3、As shown in figure 3-16 (a), loosen the belt on plate carrier, take it off from the motor at left side of
plate carrier, then upturn the plate carrier, as 3-16 (b) .
(a) (b)
Figure 3-16
4 、 Remove the two screws shown in figure 3-16 (b), you can take off the plate carrier.
Belt
(a) (b)
Figure 3-17
5、Take off the old belt and replace a new one. The replacing procedure is shown as figure 3-17 (b), here
please note that the side with tooth should face outside. In 3-18 (a) , please note that the side with
tooth should face inside.
Gear
(a) (b)
Figure 3-18
6、Install the plate carrier then upturn it, as shown in figure 3-18 (b), install the belt to the gear on the
plate carrier motor.
7、Install the metal rail and fixing bracket, adjust the belt to make the plate carrier move smoothly, then
install the shield box and top cover, restore the instrument.
17
Figure 3-19
2、Use hex wrench M3 to loosen the two fixing screws, as shown in figure 3-20.
Figure 3-20
3、Take out the convergent mirror assembly, as shown in Figure 3-21 (a)
(a) (b)
Figure 3-21
4、 Loosen the two M3 cross screws which fix the optical coupler bracket, please not that you don’t need
to take them off, as shown in figure 3-21 (b).
5、 Move away the optic coupler, take out the filter wheel, as shown in figure 3-22 (a)
18
450nm
492nm
405nm
Filter wheel
630nm
Gap
Black position
(a) (b)
Figure 3-22
6、 Replace filters according to wavelength as shown in figure 3-22 (b), please note that you can use the
gap to distinguish the hole for different filters.
7、 After changing the filters, re-install the filter wheel. Please note that the upside surface between the
optic couplers should align at the side board surface of the optical path, as shown in figure 3-23.
Figure 3-23
3.3.7. Replacement of touch panel/LCD screen
1、 Lift the top cover of instrument, remove the fixing screw on the main board, take out the main board,
as shown in figure 3-24 (a).
(a) (b)
Figure 3-24
2、 Remove the two data cable at right side of LCD form the transition board by forceps,as shown in
19
figure 3-24 (b).
3、 Remove the four screws which fix the LCD, take out the LCD and touch panel, as shown in figure 3-25
(a) and (b).
(a) (b)
Figure 3-25
4、 Replace a new touch panel of the same model, restore the instrument.
Note: please pay attention to the sides of touch panel while installation, the rough side of the touch panel
is the touch side, which should be towards outside.
Connect to
touch panel
(a) (b)
Figure 3-26
2、 Remove all the cables on LCD transition board (including LCD cable and touch panel cable), remove
the 4 screws which fix the LCD Transition board, you can take out the board.
3、 Replace a new LCD Transition board, connect the LCD cable and touch panel cable, connect all the
wires, fix the four screws, install the shield box and top cover of instrument.
3.3.9. Replacement of the front board
1、 Lift the top cover of instrument, remove the shield box on the front board, you can see the front
board, as shown in figure 3-27.
20
Figure 3-27
2、 Remove all the wires on the front board, remove the two screws as shown in above figure, you can
take out the front board.
3、 Replace a new front board, fix the screws, connect all the wires and make them in good order. Please
note that the Hoare unit must be installed above the groove of the worktable for front board. The
deviation can not be too big or there will be an error of “No positioning signal for plate carrier”.
Hoare unit
Hoare unit Groove
(a) (b)
Figure 3-28
4、 Install the shield box and top cover of instrument.
21
Figure 3-29
2、 Remove the screws which fix the main board, remove the cables connected to main board, connect
them to a new board.
3、 Install the main board, fix the screws and restore the instrument.
Lamp cable
Figure 3-30
2、 Remove the shield box, remove the two screws which fix the platform by hex wrench M4, as shown in
figure 3-31.
Platform
Figure 3-31
3、 Remove the two screws which fix the front board by cross screwdriver M3, as figure 3-32.
22
Figure 3-32
4、 Use a cross screwdriver M3 to loosen the screws which fix the optical fiber head, please note that you
do not need to take the screw out. Then fix the screw in the middle by hex wrench M3, as shown in
figure 3-33.
Figure 3-33
5、 Use a hex wrench M4 to loosen the two screws which fix the optical path, please note that you don’t
need to take them out, as shown in figure 3-34.
Figure 3-34
6、 Use a cross screwdriver M3 to remove the two screws which fix the optical fiber bracket, as shown in
figure 3-35.
23
Optical fiber bracket
Figure 3-35
7、 Lift the platform; take out the optical fiber assembly, as shown in figure 3-36.
Figure 3-36
8、 The optical fiber is as figure 3-38; Remove the four screws which fix the optical fiber, replace a new
one, as shown in 3-39.
Figure 3-38
Figure 3-39
24
9. Replace a new optical fiber with the same model, install it carefully as original, fix the optical fiber
and its head, and restore the instrument.
10. Turn on the instrument, enter the backdoor procedure, turn on the lamp, turn the filter to 492nm.
11. Find a white thin paper; put it at the eight light points to watch the faculas, as shown in figure 3-40.
The 8 faculas should be at the center position of the black hole as figure 3-40. If there is deviation,
you shall loosen the two screws which fix the optical fiber, fine tuning the position of optical fiber,
until the facula come to the center of the hole, then fix the screw again.
Figure 3-40
12. Install the platform, front board and shield box as original, install the top cover of instrument.
13. Enter the backdoor procedure of instrument, check the AD value of each channel, If the deviation is
too big, you have to adjust the position of optical fiber or the fiber is not good, you must change a
new fiber again.
14. Take calibration liquid of 2.0A to test the instrument at backdoor procedure, if it can not meet the
requirement, you shall fine tuning the position of the optic coupler on the optical fiber. We do not
suggest the engineer to replace the optical fiber at customer side.
Lamp cable
Figure 3-41
3、 Remove the two hex screws which fix the platform, lift the platform carefully (pay attention not to
pull the optical fiber), then you can see the motor driver board, as shown in figure 3-42.
Optical fiber Motor driver board
Figure 3-42
4、 Take off all the wires on motor driver board and remember their positions, remove the four screws
which fix the board, take off the motor driver board, replace a new one, and install it as original.
5、 Install the shield and top cover of instrument.
Lamp cable
Figure 3-43
3、 Remove the two hex screws which fix the platform, lift the platform carefully (pay attention not to
pull the optical fiber).
4、 Take off all the wires on the power supply and remember their positions, as shown in figure 3-44.
Remove the screws which fix the power supply, then remove the screw which fix the bottom of power
supply, change a new one and restore the instrument.
26
Figure 3-44
Note:
1、 There is some glue on the plugs, befor you take it out, you can use a tool to
remove the glue.
2、 If you are not familiar with the position of plugs on the board, you shall
remenber the cresponding positions carefully, so that you will not make
mistakes when you want to install the plug again.
4. Trouble shooting
Trouble Solution
The plate courier can not move The supporting frame in the middle of the plate courier is not
smoothly on the board and rubs properly installed. Reinstall the supporting frame.
against the optical grating board.
Report “492nm wavelength is too The lamp is aging. Replace the lamp.
weak” when start the machine. The light-filtering sheet is broken. Replace the light-filtering
sheet and adjust the frequency and synchronization of the
light-chopper wheel.
Report “shared ROM error” when Too short interval between the starts of the machine. It shall be
start the machine. above 3 minutes.
No ground wire or the ground wire is in poor contact. Earthing
the instrument properly.
The machine is started in the wrong order. You should switch
on the host computer first and then the printer.
The front-end board error. Add a 4.7K resistor to between the
20th pin and the 28th pin of U11 on the front-end board.
27
Report “405nm wavelength is too The lamp fell off or is not properly installed. Reinstall the lamp.
weak” when start the machine. The light-filtering sheet is broken. Replace the light-filtering
sheet.
The lamp is aging. Replace the lamp.
The optical fiber is damaged. Replace the optical fiber.
The photoelectric reaction board on the front-end board is
broken. Replace the photoelectric reaction board.
The clock is out of order and need to The battery is electrically discharged. Replace the battery.
be re-set up every time the machine
is started.
No display on the screen when start The flat data cable that connects the displaying screen to the
the machine. transfer board is loose or fell off. Re-plug it.
The ground wire of the transfer board fell off or is in poor
contact. Reconnect it.
The transfer board is broken or fell off. Replace it or solder it.
The displaying screen is broken. Replace the displaying screen.
The mainboard is broken. Replace the mainboard.
Report “shared ROM (RAM) error” No ground wire or the ground wire is in poor contact. Earthing
when start the machine, and then all the instrument properly.
the data stored in the machine are The machine is started in the wrong order. You should switch
lost. on the host computer first and then the printer.
The front-end board error. Add a 4.7K resistor to between the
20th pin and the 28th pin of U11 on the front-end board.
The front-end board is broken. Replace the front-end board.
Feign positive in testing HbsAg. The sample is contaminated or incompetent. Re-make the
sample.
The range of negative contrast shall be set as 0.05-3.5.
The machine’s internal K values are altered. Re-set the K values.
Set all K1s as 1 and all K2s as 0.
Report the blank absorbency is too The blank is contaminated. Use clean blank holes.
high in testing Modify the blank from ≤0.05 to ≤0.1 in item setting.
The enzyme label plate is wedged in The enzyme label plate is not properly placed or the plate bar
28
the machine and can not be taken is incompetent. Open the machine housing and take the
out, or wedged outside the machine enzyme label plate out. Use the right one.
and can not be put in for testing.
The screen suddenly becomes a The voltage is not stable. Connect it with a manostat.
blank screen, and after a while, it The flat data cable that connects the displaying screen to the
restores normal displaying. transfer board is loose. Re-plug it.
The clip hood of the plate courier is Replace the plate courier.
damaged.
The belt is broken. Replace the belt.
The printer can not print. The model of the printer is improperly set up. Re-set up the
model of the printer.
The printing cable fell off. Re-plug the printing cable.
The printer is broken. Replace the printer.
Report monotonous errors in testing The method selected in item setting shall be “Logarithmic
T3 and T4 items. regression”.
In plate arrangement, the standard articles shall be arranged in
the same line and from left to right in
Gradient disposition.
Report “no predetermined-position Too long distance between the Hall module at the bottom right
attainment signal for the enzyme corner of the front-end board and the magnetic ball of the
label plate” when start the machine. plate courier. Adjust the position of the Hall module.
The photoelectric coupler. Replace the photoelectric coupler.
The front-end board is broken. Replace the front-end board.
Report “no reset signal for the The position of the driving motor is deviated. Readjust it and fix
enzyme label plate” when start the it.
machine. The optical grating bar fell off. Glue the optical grating bar.
The drive board is broken. Replace the drive board.
Data can not be transferred to the The serial port cord is broken. Replace the serial port cord (the
computer. normal serial port twisted-wire shall be 2-3, 3-2 and 5-5).
Data transference shall be conducted in the main menu of the
software.
External software error. Reinstall the software.
Logarithmic regression testing curve The setting of 0 density standard in logarithmic regression is
is in confusion. unnecessary. Remove the 0 density standard from item setting.
In plate arrangement, the standard articles shall be arranged in
the same line and from left to right in
gradient disposition.
29
software upgrading error. Fail to start Re-upgrade the software
the machine.
When start the machine, the mouse The reverse-converter on the transfer board is damaged.
appears, but soon after, it flashes Replace the reverse-converter.
and then disappears, and the screen
becomes a blue screen.
The testing results are on the high Recommend the use of double-wavelength method of testing.
side. Select 630 as the secondary wavelength.
Report “FLASH self-check error” The Flash ROM on the mainboard is broken. Replace it.
when start the machine.
No display when printing the System software error. Update the system software.
synthetic report for the testing item.
Report “the front-end command is The power supply board’s 5V voltage is on the low side.
overtime” in the self-check when Replace the V18 on the power supply board.
start the machine.
Fail to start the machine. The lamp is Irregular power supply. Replace the power supply unit
flashing.
Report “please check the
The circuit between the 1st pin of U8 on the front-end board
light-chopper’s motor” in the
and the drive board is broken. Reconnect it.
self-check when start the machine.
30
Sample data transfer
R, %s,%s,%d,%d,%s,%03d,%s,%s,%f,%s,%s,%f,%-7.3f,%7.3f,%.4x
R, patient name,section office name,gender of patient, age,time for test,sample number,
assay name,full name,unit,qualitative,quantitative,normal range (low),normal range (high),
checkout code,TRANSFER FINISH
port:
baud rate:19200
verify:Odd
data bit:8
stop bit:1
31