2 Instrument Configuration

syringe with the silicone tube to Connector 2. Pull out Tube P6 from the L-type connector at the
top end of Connector 1. Drain the water from the sheath flow device with a syringe and pull out
Tube P21 and P4c from the Connector 3 and 4 at the bottom of the flow chamber.
Figure 2-51 Pulling out the tubing

1 - Tube P6 2 - Tube P1

3 - Tube P21 3 - Tube P4c

3. Use a #2 (Ph2) Phillips screwdriver to disassemble the four M3×8 cross-recessed pan-head
combination screws which affix the optical assembly, and take off the four large D3 gaskets.
Carefully push upwards and slowly detach the optical assembly.
Figure 2-52 Disassembling the optical assembly

1 - M3×8 cross-recessed pan-head 2 - Large D3 gasket

combination screw (×4)
4. Pull out the wires from the J66 and J4 ports on the rear panel; then pull out the heating wire
inside the optical system box, the temperature sensor wire, and the temperature switch wire. To
take the optical assembly apart, slowly hold it up and remove the flow chamber from the opening

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 2 Instrument Configuration

of the affixed optical panel.

Installation
Follow the corresponding disassembly steps in reverse order.

l Put on antistatic gloves and an electrostatic-shielding ring while operating to avoid damaging the
LED.
l All the wirings need to be set up according to the original positions of the machine components
to prevent them from being crushed or otherwise damaged.
l During transportation and installation, the flow chamber exposed at the bottom of the optical
system needs to be protected from being crushed or pressed.When pulling out the tubes, hold
the flow chamber by hand to prevent it from being dislocated by external force.

2.4.2 Replacing the Optical Preamplifier Panel

Purpose
The optical preamp panel can be disassembled and replaced by following the procedures specified
in this section; however, in each case, Preliminary Steps need to be taken before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l New optical preamplifier panel

Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. Open the right-side door and disassemble the top cover panel.
4. Take down the cover panel of the optical system box.

Disassembly
1. Pull out the three wires from the optical preamp panel.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the four M3×8 cross-recessed pan-head
combination screws which affix the optical preamp panel, and carefully detach the cover panel of
the optical system box.

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 2 Instrument Configuration

Figure 2-53 Taking down the cover panel of the optical system box

1 - M3×8 cross-recessed pan-head 2 - Optical preamplifier panel

combination screw (×4)

3 - Pre-optical fixation panel

Installation
Follow the corresponding disassembly steps in reverse order.

l Put on antistatic gloves and an electrostatic-shielding ring while operating to avoid damaging the
LED.
l All the wirings need to be set up according to the original positions of the machine components
to prevent them from being crushed or otherwise damaged.

2.4.3 Replacing the Laser Driver panel

Purpose
The laser driver panel can be disassembled and replaced by following the procedures specified in
this section; however, in each case, Preliminary Steps need to be taken before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l New laser driver panel

Preliminary Steps
1. Turn off the power switch on the left side of the device.

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 2 Instrument Configuration

2. Pull out the power cord plug from the rear panel of the device.
3. Open the right-side door and disassemble the top cover panel.

Disassembly
1. Use a 2.5mm hex-socket screwdriver to disassemble the four M3×6 stainless-steel inner hex
screws that affix the baffle plate of the laser driver panel.
2. Pull out the two wires from the laser driver panel.
3. Pull out the two wires from the laser driver panel. Use a #2 (Ph2) Phillips screwdriver to
disassemble the four M3×8 cross-recessed pan-head combination screws, and carefully detach
the laser driver panel.
Figure 2-54 Replacing the Laser Driver Panel

1 - M3×6 stainless-steel inner hex 2 - Baffle plate of the laser driver panel
screw (×4)

3 - M3×8 cross-recessed pan-head 3 - Laser driver panel

combination screw (×4)

Installation
Follow the corresponding disassembly steps in reverse order.

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 2 Instrument Configuration

l Put on antistatic gloves and an electrostatic-shielding ring while operating to avoid damaging the
LED.
l All the wirings need to be set up according to the original positions of the machine components
to prevent them from being crushed or otherwise damaged.

2.4.4 Fine Tuning of the Flow Chamber

Purpose
The fine tuning of the flow chamber can be performed by following the procedures specified in this
section; however, in each case, Preliminary Steps need to be taken before you begin.

Tools/Spare Parts
#2 (Ph2) Phillips screwdriver

Preliminary Steps
l Open the right-side door and disassemble the top cover panel.
l Take down the cover panel of the optical system box.
l Turn on the device and launch the software application; then enter the Optical Tuning screen.

Tuning Procedures
1. Use a #2 (Ph2) Phillips screwdriver to loosen the two M3×8 cross-recessed pan-head
combination screws that affix the locking plates of the flow chamber.
Figure 22-55 Fine tuning of the flow chamber

1 - M3×8 cross-recessed pan-head 2 - Adjustment knob of

combination screw (×2) the flow chamber
2. Shake the 7um nominal particles well, and use the 7um nominal particles as a sample for DIFF

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 2 Instrument Configuration

testing.
Ø If the resultant scattergram shows a square or diamond with the upper and lower sides
parallel to each other, the device does not need fine-tuning.
Ø If the scattergram shows that the upper and lower sides are not parallel, then take the next
step.
3. If the slanting directional arrow shown in the scattergram is ↗, then rotate the adjustment knob
of the flow chamber counter-clockwise; if the slanting direction shown in the scattergram is ↘,
then rotate the adjustment knob of the flow chamber clockwise (the adjustment knob should be
lightly rotated).
4. Use the 7um nominal particles as a sample for DIFF testing.
Ø If the resultant scattergram shows a square or diamond with the upper and lower sides
parallel to each other, the device does not need fine-tuning.
Ø If the scattergram shows that the upper and lower sides are not parallel, then repeat Step 3.
5. After tuning, tighten the two locking screws of the flow chamber, and reinstall the cover panel of
the optical system box as well as the four affixing screws; then lock them in place.

2.4.5 Replacement of High-angle Receiving Plate

Purpose
The high-angle receiving plate can be disassembled and replaced by following the procedures
specified in this section; however, in each case, Preliminary Steps need to be taken before you
begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l New high-angle receiving plate

Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. Open the right-side door and disassemble the top cover panel.

Disassembly
1. Disassemble 2 M3 ×8 stainless steel internal hexagon screws used for fixing the fixed seat of the
side panel card by 2.5mm internal hexagonal wrench, and then remove the fixed seat from the
side basal plate.
2. Pull out 2 connecting wires on the high-angle receiving plate.
3. Disassemble 2 M2.5 ×6 cross recess head screws used for fixing the high-angle receiving plate
by #2 (Ph2) Philips screwdriver, and remove the laser driver plate carefully.

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 2 Instrument Configuration

Figure 2-56 Replacement of High-angle Receiving Plate

1 - M3×6 inner hex screw (×2) 2 - Fixed seat of side panel card

3 - Side basal plate 4 - M2.5×6 Cross recess head screw (2x)

5 - High-angle receiving plate

2.4.6 Common Problems and Solutions

Problem Description Possible Cause Solution

There is no scattergram l The laser Check if the laser is working.

signal when testing the driver panel is l If not, replace the laser driver panel.
DIFF signal of venous not working.
blood. l If the laser is working, check the power
l The optical supply of the optical preamp panel and
preamplifier replace the optical preamp panel.
panel is not
working.

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 2 Instrument Configuration

Problem Description Possible Cause Solution

One or some of the Optical Check if the laser is working.

signals from the three preamplifier panel l If not, replace the laser driver panel.
angles are 0 when testing problem
the DIFF signal of venous l If the laser is working, check the power
blood. supply of the optical preamp panel and
replace the optical preamp panel.
If the problem persists after the replacement,
replace the main control panel.

There are only sporadic The flow chamber Check if the collecting corner plate has two
background scattergram is dislocated. bilaterally symmetric light spots in the shape of red
signals when testing the The hydraulics vertical stripes.
DIFF signal of venous have no sheath l If there is only one red vertical stripe or the
blood. flow. two stripes are significantly different in
brightness, make small adjustment to the
location of the flow chamber and return the
nominal-particle scattergram to normal.
l If there are two red vertical stripes which are
not significantly different in brightness, check
if the pinch valve is working; if it is, check if
Valve 10, 11, 12, 13, and 18 are also working.

The scattergram signals The laser is burnt Replace the laser.

are basically normal but out.
compressed in size when
testing the DIFF signal of
venous blood.

The neutral-particle signal The flow chamber Make a small adjustment to the location of the flow
(the long horizontal stripe is dislocated. chamber and return the nominal-particle
at the top right corner) in scattergram to normal.
the scattergram is
considerably slanted and
the three signals are
mostly overlapping with
inaccurate classification
when testing the DIFF
signal of fresh venous
blood.

There is a lot of noise The reagent is l If the background noise has a certain shape
when testing the contaminated. and is concentrated at the lower half of the
background signal. Tiny air bubbles scattergram, keep the DIFF1 and DIFF2
get into the fluids at room temperature and perform three
hydraulics. rounds of the reagent replacement sequence
each for the diluent, DIFF1 and DIFF2.
l If the background signal has no shape and
randomly spreads all over the scattergram,
replace the sheath flow syringe.
If the problem persists, replace the optical
assembly.

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3 Repairs

3.1 Overview
The repair engineer can repair the analyzer using standard tools. See the following section for
repairing procedures (including the tools needed). If any repairing step requires a validating step, the
repair engineer should strictly follow the procedure and take the validating step.

When following the repairing procedure as specified in this section, the operator should put on
rubber gloves and clean both hands with disinfectant after performing any repair work.

3.2 Preparatory Work Before Repairs

3.2.1 Disassembling the Left Side Panel

Purpose
While performing regular machine maintenance and inspection, the left-side door needs to be
opened to disassemble the sample syringe assembly, reagent testing panel, or electromagnetic
valve assembly, which are all in the left section of the machine.

Tools/Spare Parts
#2 (Ph2) Phillips screwdriver

Opening
1. As shown in Figure 3-1, use #2 (Ph2) cross screw driver to take out four M3×8 cross recessed
pan-head combination screws on the back panel, and take off the back panel.

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 3 Repairs

Figure 3-1 Disassembling the back panel

1 - Back panel 2 - M3×8 cross-recessed pan-head

combination screw (×4)

2. As shown in Figure 3-2, use #2 (Ph2) cross screw driver to take out three M3×8 cross recessed
pan-head combination screws on the left side panel, and take off the left side panel.
Figure 3-2 Disassembling the left side panel

1- M3×6 cross-recessed 2 - Left side panel

countersunk-head screw (×3)

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 3 Repairs

3.2.2 Open the Right Side Door

Purpose
While performing machine maintenance and inspection, the right side door needs to be opened to
disassemble the preheating bath assembly, pinch valve, sampling assembly, liquid pump assembly,
impedance counting reaction bath assembly (WBC & RBC), negative-pressure chamber, or
electromagnetic valve & pump assembly, which are all in the right section of the machine.

Tools/Spare Parts
Slot-type screwdriver

Opening
As shown in Figure 3-3, insert the slot-type screwdriver into the slot in the right side door lock and
rotate 90° counterclockwise, then manually open the right side door.
Figure 3-3 Opening the right side door

3.2.3 Disassembling the Panel Cover

Purpose
While performing regular machine maintenance and inspection, the panel cover needs to be taken
down for disassembling the sample probe, cleaning swab, sampling assembly, or indicator light
panel.

Tools/Spare Parts
#2 (Ph2) Phillips screwdriver

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 3 Repairs

Preliminary Steps
1. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
2. The left side panel has been disassembled. See 3.2.1 Disassembling the Left Side Panel.
3. The right-side door is opened. See 3.2.2 Open the Right Side Door.

Disassembly
1. Disassembly the back panel: use a #2 (Ph2) Phillips screwdriver to disassemble the three M3×6
cross-recessed pan-head combination screw on the back panel, and take off the back panel, as
shown in Figure 3-4.
Figure 3-4 Disassembling the back panel

1 - Right side door 2 - M3×8 cross-recessed

countersunk-head screw (×3)

2. Diassembling top cover: Open the right side door and disassemble 4 M3×8 cross-recessed
countersunk-head screws on both sides of top cover by #2 (Ph2) Philips screwdriver, the top
cover can be removed, as shown in Figure 3-5.

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 3 Repairs

Figure 3-5 Disassembling the top cover panel

1 - Top cover panel 2 - M3×8 cross-recessed countersunk-head

screw (×4)
3. Take off the panel cover: as shown in Figure 3-6, take out the six affixing M3×8 cross-recessed
countersunk head screws in order to take off the panel cover.

When removing the panel cover, make sure to pull out the wires connected to the display circuit
panel on the panel cover.

Figure 3-6 Disassembling the panel cover

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 3 Repairs

1 - Panel cover 2 - M3×8 cross-recessed

countersunk-head screw (×6)

3.3 Display Screen Assembly Replacement

3.3.1 Replacing the Touch Screen

Purpose
The display screen can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you
begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l Display screen

Disassembly
1. Pull out both interfaces at the shield panel of the LCD screen interface panel, use #2 (Ph2) cross
screw driver to remove four M3×8 cross recessed pan-head combination screws on the shield
panel, take off the shield panel, then detach the black buckles on both ends, gently pull out the
golden ribbon cable, as shown in Figure 3-7.
Figure 3-7 Replacement of display screen (1)

1 - Bunch interface 2 - LCD screen interface

panel

3 - M3×8 cross recessed pan-head 4 - Ribbon cable interface

combination screw (×4)

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 3 Repairs

2. Use #2 (Ph2) cross screw driver to remove eight M3×8 cross recessed pan-head combination
screws on the screen support, take off the screen support, display screen and touch screen
respectively, as shown in Figure 3-8.
Figure 3-8 Replacement of display screen (2)

1 - Touch screen 2 - Display screen

3 - Screen support 4 - M3×8 cross recessed pan-head

combination screw (×8)

3.4 Sampling Assembly Replacement

3.4.1 Replacing the Sample probe

Purpose
The sample probe can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you
begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l Sample probe

Disassembly
1. Pull out the swab's snap ring to detach it from the lower bracket panel. See Figure 3-9.

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 3 Repairs

Figure 3-9 Replacement of sample probe (1)

1 - Cleaning swab 2 - Swab circlip

2. Use a #2 (Ph2) Phillips screwdriver to take out the two M3×6 small cross recessed pan head
combination screws on the fixed pressure plate of the sample probe, and then take off the fixed
pressure plate. See Figure 3-10.
Figure 3-10 Replacement of sample probe (2)

2 - Pressure plate affixing the

1 - Sample probe
sample probe

3 - M3×8 cross-recessed pan-head

combination screw (×2)

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 3 Repairs

3.4.2 Replacing the Optocoupler

Purpose
The optocoupler can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you
begin.

Tools/Spare Parts
l 2.5mm hex-socket screwdriver
l Optocoupler

Disassembly
Use the 2.5mm hex wrench to remove the M3 hex screw affixing the optocoupler, then pull out the
plug attached to the optocoupler wire to disassemble the optocoupler to be replaced. See Figure
3-11.
Figure 3-11 Replacement of optocoupler

1 - Optocoupler 2 - Sampling assembly

3.4.3 Replacing the Sampling Assembly in X- or Y-direction

Purpose
The sampling assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before
you begin.

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 3 Repairs

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l diagonal cutting pliers

Disassembly
1. Use the diagonal cutting pliers to cut off all the nylon binding tapes affixing the fluid tubes
(aspiration tubes of the sample probe and swab tubes), then pull out the fluid tubes of the sample
probe and the swab to detach them from the sampling assembly.
2. Pull out all the motor wires and optocoupler plug from the horizontal moving module of the
sample assembly, and use a #2 (Ph2) Phillips screwdriver to take out the three M3×6 cross
recessed countersunk head screws affixing the bracket for drag chains to detach the
sampling-specific drag chain from the sampling assembly. See Figure 3-12.
Figure 3-12 Replacement of the sampling assembly in X- or Y-direction (1)

1 - tank drag chain 2 - M3×6 cross-recessed

connector countersunk-head screw (×3)
3. Use a #2 (Ph2) Phillips screwdriver to take out the four M4×10 cross recessed pan head
combination screws affixing the sampling assembly, and then carefully and gently take the
sampling assembly out of the machine; during the process of removal, carefully pull out the wires
and plugs for the horizontal motor and optocoupler of the sampling assembly from the
corresponding apertures in the machine. Then pull out all the plugs to completely remove the
sampling assembly. See Figure 3-13.

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 3 Repairs

Figure 3-13 Replacement of the sampling assembly in X- or Y-direction (2)

1 - Sampling assembly 2 - M4×10 cross-recessed pan-head

combination screw (×4)

3.5 Power Assembly Replacement

Purpose
The power assembly can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you
begin.

Tools/Spare Parts
l Phillips screwdriver
l Power assembly with the same specifications

Disassembly
1. Use Phillips screwdriver to take out four M3×8 cross recessed pan-head combination screws on
the back of the device power supply assembly, then remove one M3×8 cross recessed pan-head
combination screw on the front, as shown in Figure 3-14.

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 3 Repairs

Figure 3-14 Replacement of power assembly (1)

1 - Power assembly 2 - M3×8 cross-recessed pan-head

combination screw (×5)
2. Unplug all the connectors attached to the lead wire of the power assembly.
3. Take off the power supply required to be replaced from the power supply assembly, and replace
with a new power supply, fix it with screws.

3.6 Hydraulics Components incl. Valves, Pumps, and

Pressure Chambers Replacement

3.6.1 Replacing the valve assembly

Purpose
The valve assembly can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you
begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l The replacement valve with the same specifications

Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. If it is only the electromagnetic valve on the left or right clapboard that needs to be replaced,

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 3 Repairs

open the corresponding left- or right-side door; if the one on the front panel needs to be replaced,
remove the front panel cover by following the instructions in 3.2.3 Disassembling the Panel
Cover.

Disassembly
1. Disassemble the peripheral fluid tubes connected to the valve assembly.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the two M3×8 cross-recessed pan-head
combination screws which affix the valve assembly, and carefully take out the valve assembly
while making sure to unplug the attached wires. See Figure 3-15.
Figure 3-15 Replacement of valve assembly

1 - the corresponding replacement valve 2 - Installation panel affixing the valve

3 - M3×8 cross-recessed pan-head

combination screw (×2)

Installation

l Make sure to the use correct model of valve and create a reliable connection
l All the wirings need to be set up according to the original positions of the machine components
to prevent them from being crushed or otherwise damaged.
l This machine involves a great number of valves. Only one valve is discussed as an example
here, and all the other valves follow the same maintenance procedures.

Follow the corresponding disassembly steps in reverse order.

3.6.2 Replacing the Liquid Pump Assembly

Purpose
The liquid pump assembly can be disassembled and replaced by following the procedures specified

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 3 Repairs

in this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken
before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l The replacement liquid pump with the same specifications

Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. Open the right side door.

Disassembly
1. Pull out the peripheral fluid tubes and wire connectors attached to the liquid pump.
2. Use a #2 (Ph2) Phillips screwdriver to loosen the two M4×10 cross-recessed pan-head
combination screws which affix the liquid pump, and move the liquid pump assembly to the left till
the big hole on the affixing panel of the liquid pump is aligned to the affixing screw, then carefully
hold up the liquid pump assembly to take it out. See Figure 3-16.
Figure 3-16 Replacement of liquid pump assembly (1)

1 - Liquid pump 2 - M4×10 cross-recessed pan-head

assembly combination screw (×2)

3. The further dismantling of the liquid pump assembly is shown in Figure 3-17.

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 3 Repairs

Figure 3-17 Replacement of liquid pump assembly (2)

1 - Liquid pump 2- M3×8 stainless-steel inner hex screw (×8)

Installation

l All the wirings need to be set up according to their original positions to prevent them from being
crushed or otherwise damaged, and to prevent the working liquid pump from being shaken,
which affects the fluid tubes.
l Pay special attention to the tubing joints and ensure that the connections are sound.

Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Close the right side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

3.6.3 Replacing the Negative-pressure Chamber Assembly

Purpose
The negative-pressure chamber assembly can be disassembled and replaced by following the
procedures specified in this section; however, in each case, 3.2 Preparatory Work Before Repairs
need to be taken before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l The replacement parts or assembly of the negative-pressure chamber with the corresponding
specifications

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 3 Repairs

Preliminary Steps
Open the right side door.

Disassembly
1. Disassemble the peripheral fluid tubes connected to the negative-pressure chamber.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the two M4×10 cross-recessed pan-head
combination screws which affix the negative-pressure chamber, and carefully take out the
negative-pressure chamber assembly. See Figure 3-18.
Figure 3-18 Replacement of negative-pressure chamber assembly

1 - Negative-pressure 2 - M4×10 cross-recessed pan-head

chamber assembly combination screw (×2)
3. The further dismantling of the negative-pressure chamber assembly is the same as that of the
positive-pressure chamber assembly.

Installation

l Make sure to place the seal ring into the seal tank to keep the pressure chamber airtight.
l All the tubing needs to be set up according to the relevant standards or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.

Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

Close the right side door.

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 3 Repairs

3.7 Sheath Flow Syringe Assembly Replacement

3.7.1 Replacing the syringe

Purpose
The syringe can be disassembled and replaced by following the procedures specified in this section;
however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before you begin.

Tools/Spare Parts
l 2.5mm hex-socket screwdriver
l The replacement syringe assembly with the same specifications

Preliminary Steps
l Open the left side door.

Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe assembly to be replaced.
2. Use the 2.5mm hex-socket screwdriver to disassemble the two M3×8 stainless-steel inner hex
screws affixing the syringe assembly. Then take out the syringe assembly. See Figure 3-19.
Figure 3-19 Replacement of syringe

2
1

1- Syringe module 2- M3×8 stainless-steel inner hex screw (×2)

Installation
Follow the corresponding disassembly steps in reverse order.

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 3 Repairs

3.7.2 Replacing the Motor

Tools/Spare Parts
l 2.5mm hex-socket screwdriver
l #2 (Ph2) Phillips screwdriver
l The replacement syringe assembly with the same specifications

Preliminary Steps
Open the left side door.

Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe module to be replaced.
2. Use #2 (Ph2) cross screw driver to remove four M3×8 cross recessed pan-head combination
screws, take off the syringe assembly. See Figure 3-20.
Figure 3-20 Replacement of motor (1)

1
2

3. Use the 2.5mm hex-socket screwdriver to disassemble the two M3×8 stainless-steel inner hex
screws affixing the syringe module. Then take out the syringe. See Figure 3-19.
4. Take out the two M3×8 inner hex screws on the shield cover, then remove the white bolt from the
motor. Next, remove the four M3×8 inner hex screws from the back of the motor for the
replacement. See Figure 3-21.

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 3 Repairs

Figure 3-21 Replacement of motor (2)

1 - Motor bolt 2 - Motor

3 - M3×8 stainless-steel inner hex screw (×9)

Installation
Follow the corresponding disassembly steps in reverse order.

3.8 WBC and RBC Bath Assemblies Replacement

3.8.1 Dismantling and replacing the WBC bath assembly

Purpose
The WBC counting bath assembly can be disassembled and replaced by following the procedures
specified in this section; however, in each case, Preliminary Steps needs to be taken before you
begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l The replacement WBC counting bath assembly with the same specifications

Preliminary Steps
1. Run the draining sequence of the software to drain any residual liquid inside the machine.
2. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
3. Open the right side door.

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 3 Repairs

Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard. See Figure 3-22.
Figure 3-22 Dismantling and replacing the WBC bath assembly (1)

1 - Shield cover of the counting bath 2 - Cross-recessed pan-head combination screw

2. Pull out the peripheral fluid tubes attached to the WBC counting bath assembly. Pay attention to
handling the residual fluid; try to prevent it from flowing onto the other components in the
machine, and wipe clean if necessary.
3. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw for grounding on the middle clapboard of the shield case cover and pull out
the grounding wire. See Figure 3-23.
Figure 3-23 Dismantling and replacing the WBC bath assembly (2)

1 - WBC counting chamber 2 - M3×8 cross-recessed pan-head

assembly combination screw

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 3 Repairs

4. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel hex-socket screws affixing the
upper section of WBC counting bath assembly. See Figure 3-24.
Figure 3-24 Dismantling and replacing the WBC bath assembly (3)

1 - WBC counting chamber assembly 2 - M3×8 stainless-steel inner hex screw (×2)
5. Carefully and slowly take out the WBC counting bath assembly.Pay attention to the wiring in the
process of moving to avoid breaking the wires.
6. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire.Then take out the WBC counting bath assembly as a
whole.
7. The further dismantling of the WBC counting chamber assembly. See Figure 3-25.
Figure 3-25 Dismantling and replacing the WBC bath assembly (4)

1 - Front counting bath (WBC) 2 - HGB emission tube holder

3 - LED 4 - HGB receving tube holder
5 - Optical filter 6 - Optical receiver

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 3 Repairs

Installation

l All the wirings need to be set up according to the working procedures or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.
l Pay special attention to the tubing joints and ensure that the connections are sound.
l The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Reinstall the shield case cover.
2. Close the right side door.
3. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

3.8.2 Dismantling and replacing the RBC bath assembly

Purpose
The RBC counting bath assembly can be disassembled and replaced by following the procedures
specified in this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be
taken before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2.5mm hex-socket screwdriver
l The replacement WBC counting bath assembly with the same specifications

Preliminary Steps
1. Run the draining sequence of the software to drain any residual liquid inside the machine.
2. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
3. Open the right side door.

Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard.
Refer to the corresponding section in 3.8.1 Dismantling and replacing the WBC bath
assembly.
2. Pull out the peripheral fluid tubes attached to the RBC counting bath assembly.

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 3 Repairs

Pay attention to handling the residual fluid; try to prevent it from flowing onto the other
components in the machine, and wipe clean if necessary.
3. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw for grounding on the middle clapboard of the shield case cover and pull out
the grounding wire. See Figure 3-26.
Figure 3-26 Dismantling and replacing the RBC bath assembly (1)

1 - RBC counting chamber 2 - M3×8 cross-recessed

assembly pan-head combination screw
4. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel inner hex screws affixing the
upper section of the RBC counting bath assembly. See Figure 3-27.
Figure 3-27 Dismantling and replacing the RBC bath assembly (2)

1 - RBC counting chamber assembly 2- M3×8 stainless-steel inner hex screw (×2)

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 3 Repairs

5. Carefully and slowly take out the RBC counting bath assembly. Pay attention to the wiring during
the process of moving to avoid breaking the wires.
6. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire. Then take out the RBC counting bath assembly as a
whole.
7. The further dismantling of the RBC counting chamber assembly. See Figure 3-28.
Figure 3-28 Dismantling and replacing the RBC bath assembly (4)

1 - Front counting bath 2 - Flat rubber gasket 6*4.5*0.5

3 - RBC aperture 4 - Flat rubber gasket 6*3*0.5

5 - O-shaped ring 5.5*1.0 6 - Electrode of the rear bath

7 - O-shaped ring 6.5*1.0 8 - Rear chamber

Installation

l All the wirings need to be set up according to the working procedures or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.
l Pay special attention to the tubing joints and ensure that the connections are sound.
l The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Reinstall the shield case cover.
2. Close the right side door.
3. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

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 3 Repairs

3.9 Main Control Panel Replacement

Purpose
The main control panel can be disassembled and replaced by following the procedures specified in
this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before
you begin.

Tools/Spare Parts
l Phillips screwdriver
l Corresponding specifications needed to replace the main control panel

Preliminary Steps
1. Pull out the power cord plug from the rear panel of the analyzer, and turn off the power switch.
2. Disassemble the screws on the back panel of housing and remove the back panel of the
housing.

Disassembly
1. Pull out the peripheral wires and connectors attached to the main control panel.
2. As shown in Figure 3-29, disassemble four M4×8 cross recessed pan-head combination screws
fixing the main control panel, and remove the main control panel.
Figure 3-29 Replacement of the main control panel

1
2

1 - Main control panel 2 - M3×8 cross-recessed pan-head

combination screw (×4)

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 3 Repairs

Installation
Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Assemble the back panel of housing.
2. Plug the power cord into the back panel of the device and turn on the power switch.

3.10 Reagent Testing Panel Replacement

Purpose
The reagent testing panel can be disassembled and replaced by following the procedures specified
in this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken
before you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l The replacement reagent testing panel with the same specifications

Preliminary Steps
1. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
2. Open the left side door.

Disassembly
1. Remove the four M3×8 cross recessed pan-head combination screws affixing the protective
cover of the reagent testing panel and remove the protective cover. See Figure 3-30.
Figure 3-30 Replacement of reagent testing panel (1)

1 2

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 3 Repairs

1- Protective cover 2 - M3×8 cross-recessed pan-head

combination screw (×4)
2. Pull out all the exposed peripheral wires connected to the reagent testing panel.
3. Remove the four M3×8 cross-recessed pan-head combination screws affixing the reagent testing
panel and carefully take out the reagent testing panel. Make sure that the metal parts will not
scratch the wiring at the rear of the panel. Pull out the rear wiring to remove the reagent testing
panel. See Figure 3-31.
Figure 3-31 Replacement of reagent testing panel (2)

1 - Reagent detection panel 2 - M3×8 cross-recessed pan-head

combination screw (×4)

Installation

l All the wirings need to be set up according to the working procedures or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.
l Pay attention to the position of wires when installing the sensor panel so as to prevent the wires
from breaking.

Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Close the corresponding left side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

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 3 Repairs

3.11 Temperature Sensor and Sheath-flow Pressure

Sensor Replacement

3.11.1 Disassembling and Replacing the Temperature Sensor

Purpose
The temperature sensor can be disassembled and replaced by following the procedures specified in
this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before
you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2 hex-socket screwdriver
l The replacement temperature sensor with the same specifications

Preliminary Steps
1. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
2. Open the left side door and the front panel cover.

Disassembly
Loosen the screws in the bracket for temperature sensor to take the sensor out. Then replace it with
a new sensor and tighten the screws. See Figure 3-32.
Figure 3-32 Disassembling the temperature sensor

1 - Temperature sensor 2 - Temperature sensor bracket

3 - M3×8 fixation screws

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 3 Repairs

Installation
Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Re-install the front panel cover, and close the corresponding left side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

3.11.2 Disassembling and Replacing the Sheath-flow Pressure

Sensor

Purpose
The temperature sensor can be disassembled and replaced by following the procedures specified in
this section; however, in each case, 3.2 Preparatory Work Before Repairs need to be taken before
you begin.

Tools/Spare Parts
l #2 (Ph2) Phillips screwdriver
l 2 hex-socket screwdriver
l The replacement temperature sensor with the same specifications

Preliminary Steps
1. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
2. Open the left side door and the front panel cover.

Disassembly
Unplug the wire stock and tubing connected to the pressure sensor, and take the pressure sensor
out. Then replace it with a new sensor. See Figure 3-33.

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 3 Repairs

Figure 3-33 Disassembling the sheath-flow pressure sensor

1 - Sheath-flow pressure 2 - Sheath-flow pressure sensor

sensor connecting tubing

3 - Sheath-flow pressure
sensor connecting wire

Installation
Follow the corresponding disassembly steps in reverse order.

Resetting the Machine

1. Re-install the front panel cover, and close the corresponding left side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
panel of the device.

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 4 Software Upgrade

4 Software Upgrade

4.1 Preparation
1. Get officially provided upgrade package, and prepare a USB flash disk for storing the upgrade
package.

l Please adopt USB flash disks manufactured by legitimate manufacturers.

l The USB flash disk capacity shall be larger than 1G, and the format is FAT32.

2. Copy the upgrade package to the root directory of the USB flash disk (Do not paste it into any
newly-created folder).
3. Insert the USB flash disk with upgrade package into the USB interface of the host.
4. Use service authority to log in to the host.

4.2 Upgrading Steps

1. Enter the Service interface, and click the Upgrade button, as shown in the picture below.

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 4 Software Upgrade

The host will detect the USB flash disk. 2~5 seconds later, if the USB flash disk cannot be
recognized, there will be corresponding prompt in the interface, please insert the USB flash disk
again; if the USB flash disk can be recognized, there will be dialog box in the interface as follows.

2. Select the upgrade file upgrade_1104_vxxx_XXXXXXXXXXXX.tar, and click Yes.

vxxx indicates the version of the upgrade file, and XXXXXXXXXXXX indicates the release date
of the upgrade file.
The system will pop up a similar interface as follows, listing the modules able to be upgraded and
their versions.

Meaning of the name of each module:

Ø LS: System boot program version
Ø OS: Operating system version
Ø HFPGA: FPGA program version
Ø DMCU: MCU program version
Ø AS: Application version
3. Click Yes to start the upgrade; click No to cancel the upgrade and restart the analyzer.

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 4 Software Upgrade

There will be prompt with the upgrading progress on the screen. If the upgrade is successful, the
progress bar will display 100%. See the picture below.

4. Turn off the power supply of the analyzer, and restart the analyzer.

4.3 Touch Screen Calibration

After replacing with a new touch screen, it is necessary to calibrate the touch screen prior to use,
steps for calibration are as follows:
1. Prepare a USB flash disk with FAT32 format, create a new ts.txt file under its root directory.
2. Insert the USB flash disk into the USB interface on the analyzer.
3. Turn on the device power supply, and start the analyzer.
4. When the login interface is displayed on the LCD screen, press and hold the sample Aspirate key
for more than 15s.
There will be black calibration interface displayed on the LCD screen, as shown in the picture
below.

5. Use a hard object, such as a toothpick or pencil to click the focal points successively appearing
on the screen (5 points in total).
After clicking 5 focal points, the touch screen calibration is complete.
6. Turn off the power supply of the analyzer, and restart the analyzer.

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 5 Comprehensive Device Tuning

5 Comprehensive Device Tuning

Since the replacement and maintenance of some components can lead to changes in relevant tuning
parameters, this section introduces the tuning procedures for the parameters that may be affected.

5.1 Position Adjustment

NOTE: The swab height needs to be reset after the replacement of the sampling assembly and
relevant parts of the sampling swab.
After the replacement of the sampling assembly or swab, perform the following tuning procedures.
1. Enter the Service screen and click Position Adjustment.
2. Click Start and check the distance between the bottom of the sample probe and the bottom of
the swab. If the sample probe is lower than the swab, click Up; if higher, click Down. Each click
will move the sample probe slightly.In the end, the bottom of the sample probe will be aligned
with the bottom of the swab. Then click "OK".

3. Next, click Check to run the initialization of the sample probe. Check if the bottom of the sample
probe is still aligned with the bottom of the swab (see Figure 5-1). If it is, click OK to complete the
tuning; if not, repeat Steps 1~2.

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 5 Comprehensive Device Tuning

Figure 5-1 Position Adjustment

5.2 HGB Voltage Gain Setting

NOTE: The HGB voltage gain setting needs to be performed for the replacement of WBC bath, HGB
wire and/or main control panel.
1. In the Setup > Meterage screen, click Gain Settings.
2. Click the up/down adjustment button after the filling blank for HGB value and HGB background
voltage value will change accordingly within 4.5+/-0.3V.
3. Click OK (see Figure 5-2).
Figure 5-2 HGB voltage gain setting

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 5 Comprehensive Device Tuning

5.3 Gain Calibration

NOTE: Gain calibration needs to be performed for the replacement of RBC bath and/or main control
panel.
1. Click Service > Gain cal. to access the gain calibration screen. See Figure 5-3.
Figure 5-3 Gain calibration

2. Fill in the cell corresponding to MCV with the MCV reference value for quality control.
3. Perform the QC test for three times in a row for the first run.
The results for each time will be automatically displayed.
Ø If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the first run. Go to step 4.
Ø If it does not fall within reasonable parameters, you’ll be prompted to redo the calibration.
Please click OK to close the message box. Then click Clear to delete the data, and repeat
the step 3.
4. Perform the QC test for three times in a row for the second run.
The results for each time will be automatically displayed.
Ø If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the second run, and show the final results. Please click "OK" to complete gain calibration.
Ø If it does not fall within reasonable parameters, you’ll be prompted to redo the calibration.
Please click OK to close the message box. Then click Clear to delete the data, and repeat
the step 4.

5.4 Calibration of Calibrators

NOTE: The calibrators need calibration for the replacement of the WBC bath, the RBC bath, and/or
the main control panel.

5.4.1 Calibration in Whole-blood Mode

1. In standby mode, click Cal to enter the calibration screen.
2. Select Calibrator (see Figure 55-4).

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 5 Comprehensive Device Tuning

Figure 55-4 Auto Calibration Using Calibrators

3. Input the calibrator lot No. JZQX-01 in the text box for Lot No., and click the Exp. Date control to
set the expiry date of the calibrator.
4. Input the target value of the current calibrator parameter.
The calibration mode is Whole Blood by default, so there is no need to set the mode.
5. Set the well-shaken calibrator under the sample probe, then press the aspiration key on the
analyzer to start the calibration counting.
6. Repeat step 5 for a total of 12 times to get 12 results of calibration counting.
Ø After the counting is complete, a dialog box will pop up indicating that the test is complete.
Click the Save button to save the calibration result.
Ø If significant differences are found across results, a dialog box for data abnormality will pop
up.Please redo the calibration.

5.4.2 Calibration in Predilute Mode

1. In standby mode, click Cal to enter the calibration screen.
2. Select Predilute on the right side of the screen.
3. Input the calibrator lot No. JZYXS-01 in the text box for Lot No., and click the Exp. Date control
to set the expiry date of the calibrator.
4. For calibration using the prediluted calibrators, please refer to steps 4~6 in whole-blood mode.

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 5 Comprehensive Device Tuning

5.5 LIS Connection

If the analyzer needs to be connected to laboratory information system (hereinafter referred to as
LIS), you can complete the connection by following the steps in this section.

5.5.1 Installing LIS Workstation

1. Install LIS workstation and set instrument type and model.
2. Enter LIS workstation network setup interface after installation and set monitoring IP address and
port number.

Refer to Description of LIS Communication Protocol for Dymind Hematology Analyzers to

complete the support of the LIS workstation to the LIS communication protocol.

5.5.2 Host Communication Settings

1. Use a network cabel to connect the analyzer to LIS local area network.
2. Log on the auto hematology analyzer software; if the analyzer is turned on, skip this step.
The whole process lasts for 4 to 12 minutes. Please be patient.
3. In the Setup interface, click Host Communication in the Communication selection to access
the Laboratory Information System (LIS) communication setting interface. See Figure 5-5.
Figure 5-5 Host Communication Settings

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 5 Comprehensive Device Tuning

4. Set the IP address and other network information of the analyzer according to the actual
situation.
Ø If the network is accessed through a router on the site, please select Obtain an IP address
automatically and Obtain DNS server address automatically.
Ø If the network is accessed through a network switch, or the analyzer is directly connected to
the LIS on the site, please select Use the following address, so as to manually set the IP
address and subnet mask of the analyzer. The IP addresses of the analyzer and LIS must be
in the same network segment. Furthermore, their subnet masks shall be the same, while
other parameters can maintain null.
5. Click OK to save the settings and close the dialog box.

5.5.3 Connecting Analyzer with LIS

1. Log on the auto hematology analyzer software; if the analyzer is turned on, skip this step.
The whole process lasts for 4 to 12 minutes. Please be patient.
2. In the Setup interface, click LIS Communication in the Communication selection to access the
Laboratory Information System (LIS) communication setting interface.
See Figure 5-6.
Figure 5-6 LIS Communication Settings

3. Input the IP address and port of LIS workstation in Network Settings area.
Find the IP address and port of LIS in the network setup interface in the LIS workstation; if IP
address can’t be found, try the method below:

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 5 Comprehensive Device Tuning

a. Enter the operating system of LIS workstation.

b. Press combination key [Windows+R] to open the Run window.
c. Input cmd, and then click OK.
d. Input the ipconfig command into the cmd.exe window popped out.
The interface shows similar content as follows:

The IPv4 address in the red box is the IP address of LIS workstation.

The IP address 192.168.8.44 of the LIS workstation shown as above is used as an example, real IP
should be in the same network segment with LIS server.

4. Click OK to save the settings.

5. Check if the connection is successful.

The LIS icon in the upper right side on the analyzer screen turns from gray to black ,
which indicates auto hematology analyzer software is connected to LIS successfully.
If the icon stays gray, the connection fails. Please check if the IP address and port of LIS is
correct and reconnect as the steps above; if the problem still exists, please contact the hospital
network administrator to handle it.

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 6 Alarms and Solutions

6 Alarms and Solutions

This section introduces error messages that can appear in the analyzer, possible causes, and
troubleshooting steps to be taken by the operator. If the problem persists after troubleshooting,
please take hardware issues into account and consider replacing the relevant parts or panels.
For the following issues, please click the error message box at the bottom right corner of the
software interface, then click "Remove Error" in the popup dialog box. Usually the problem will be
automatically resolved; if it persists, refer to the "Solution" column for further maintenance.

Problem Problem Solution

Problem Description
No. Name

The syringe fails to This syringe problem can occur while it is being
leave its initial moved.Please refer to the following solution:
position. 1. Follow the instructions in 3.7 Sheath Flow Syringe
Syringe fails to return Assembly Replacement to disassemble the
to its initial position. syringe, then remove the dust cover and
optocoupler. Plug the optocoupler into the connector
The syringe takes too of the syringe optocoupler. The user enters the
many steps to return Status > Sensor screen and covers the center of
Syringe
1 to its initial position. the optocoupler with a piece of paper. Check if the
problem
optocoupler status shown in the screen is blocked; if
The sample syringe yes, then the optocoupler is working properly.
is busy.
2. Follow the instructions in 3.7.2 Replacing the
Motor to disassemble the syringe motor and replace
it with a new motor. Then go to syringe self-test
Sample syringe under Service > Self-test > Syringe; if the syringe
timeout is working, then the maintenance has been
successful.

The horizontal motor 1. The user enters the Status > Sensor screen and
fails to leave its initial covers the center of the optocoupler with a piece of
position. paper. Check if the optocoupler status shown in the
screen is blocked. If it is, then the optocoupler is
The horizontal motor working properly; if not, then refer to 3.4.2
fails to return to its Replacing the Optocoupler on how to replace the
Horizontal initial position. optocoupler.
2 motor
problem The motor fails to 2. Refer to 3.4.3 Replacing the Sampling Assembly
move to the WBC in X- or Y-direction to disassemble the sampling
position. assembly and make sure the motor wiring is secure.
If the wiring is OK, then disassemble the motor and
The motor fails to replace it with a new one. After the installation, make
move to the RBC proper adjustments to the belt's tension, then go to
position. the syringe self-test under Service > Self-test >

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 6 Alarms and Solutions

Problem Problem Solution

Problem Description
No. Name

The motor fails to Syringe. If the syringe is working, then the

move to the open maintenance process is OK.
sampling position.

The horizontal motor

is busy.

Horizontal motor
timeout

The optocoupler of
the horizontal motor
is not working
properly.

The vertical motor 1. The user enters Status > Sensor screen and covers
fails to leave its initial the center of the vertical optocoupler with a piece of
position. paper. Check if the corresponding optocoupler
status shown in the screen is blocked. If it is, then
The vertical motor the optocoupler is working properly; if not, then refer
fails to return to its to 3.4.2 Replacing the Optocoupler on how to
initial position. replace the optocoupler.
The motor fails to 2. Refer to 3.4.3 Replacing the Sampling Assembly
move into position to in X- or Y-direction to disassemble the motor from
isolate the air the sampling assembly and make sure motor wiring
bubbles. is secure. If the wiring is OK, then disassemble the
motor and replace it with a new one. After the
Vertical The motor fails to installation, make the proper adjustments to the belt
3 motor move to the DIFF tension, then go to the syringe self-test under
problem bath position. Service > Self-test > Syringe. If the syringe is
working, then it has been successfully fixed.
The motor fails to
move to the counting
bath position.

The motor fails to

move to the open
sampling position.

The vertical motor is

busy.

Vertical motor
timeout

The CC Refer to 3.9 Main Control Panel Replacement to

source The CC source replace the main control panel.
4
voltage is voltage is abnormal.
abnormal

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 6 Alarms and Solutions

Problem Problem Solution

Problem Description
No. Name

Refer to 2.4.1 Integral Replacement of the Optical

Assembly to disassemble the optical shield cover and
see if the optical laser is on with normal power input
The laser (the normal measurement range of the optical power
Abnormal laser
5 current is meter is 4.8+/-0.5). If the optical laser is not on or the
current.
abnormal measured optical power is far lower than 4.8, this
means that the laser is burnt out. Please refer to 2.4.1
Integral Replacement of the Optical Assembly to
replace the entire optical assembly.

The 12V +12V power is not Refer to 3.9 Main Control Panel Replacement to
power is working properly. replace the main control panel.
6
not working -12V power is not
properly working properly.

1. Take another measurement after performing the

soaking with probe cleanser to see if the problem
has been resolved. If not, enter into the system for
multiple times to check HGB background voltage. If
the voltage is steady and exceeds the standard
Abnormal HGB ratings, please perform the following procedures.
background voltage. 2. Go to Setup > Host Settings > Gain Settings
(The rated range of screen, adjust the current HGB gain and set HGB
background voltage background voltage within 4.5+/-0.1V.
Abnormal
7 background is 4.2~4.8V; a system 3. If the problem persists, please try to clean the
voltage message "abnormal transmitting end and the receiving end of the HGB
background voltage" bracket. These two areas should not be cleaned with
will be shown to alcohol or organic solvents. Instead, use a rubber
remind the user to pipette bulb for purging. If the counting bath is
adjust HGB gain.) contaminated with overflown liquid, wipe it clean with
tissue paper that will not flake.
4. If the problem persists after the above steps are
taken, please consider replacing any relevant
components such as the HGB bracket or analog
panel.

Check if the ambient temperature is within the

The ambient
specified range of 15~30°C; if yes, refer to 3.11.1
temperature exceeds
Abnormal Disassembling and Replacing the Temperature
the working range.
8 temperatur Sensor to replace the temperature sensor.
e Preheating bath Check if the wiring between the temperature sensor
temperature out of and the heating plate in DIFF bath is loose. If the
working range connection is OK, replace the DIFF bath.

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 6 Alarms and Solutions

Problem Problem Solution

Problem Description
No. Name

Check if the wiring between the optical temperature

sensor and the heating rod in the DIFF bath is loose; If
the connection is OK, use a thermometer to measure
Optical system the temperature inside the aperture at the top of the
temperature out of front-end optical assembly.Check if the temperature is
working range. within the working range.
If it is not, replace the heating rod; otherwise, replace
the temperature sensor.

The Refer to 2.2.12.9 Problems with Creating Positive

positive-pressure Pressure for troubleshooting.
chamber fails to
create pressure

Abnormal pressure of
positive-pressure
chamber (lower than
normal)

Abnormal pressure of
positive-pressure
chamber (higher than
Pressure normal)
9 chamber
problem The Refer to 2.2.12.10 Problems in Creating the
negative-pressure Negative Pressure for troubleshooting.
chamber fails to
create pressure

Abnormal pressure of
negative-pressure
chamber (lower than
normal)

Abnormal pressure of
negative-pressure
chamber (higher than
normal)

Flow cell clog. Refer to 2.2.12.4 Clogging of the Flow

Clogging of Chamber/DIFF Probe to clean the flow chamber or
flow DIFF probe for multiple times. If the problem persists,
10
chamber/DI DIFF probe clogging. refer to 3.11.2 Disassembling and Replacing the
FF probe Sheath-flow Pressure Sensor to replace the
sheath-flow sensor.

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 6 Alarms and Solutions

Problem Problem Solution

Problem Description
No. Name

1. Check if the reagent has expired or is contaminated.

2. Go to the Service > Maintenance > Maintain
screen and click cleanser soaking to clean the
hydraulics. Then return to the Sample Analysis
Abnormal screen and conduct background measurements to
background. see if the problem has been resolved.
Abnormal (One or multiple 3. If the problem persists, please check for any
11 results of background peripheral interference from the grounding wire or
background
measurement shield wire, or if any electric-brush devices such as
exceed the drills are being used on and off in the area.This can
background range.) influence the counting results.
4. If there is no such interference, please check the
airtightness of each syringe and the rear chamber of
the counting bath.If the airtightness is unsatisfactory,
then replace accordingly.

1. Go to the Service > Maintenance > Maintain

screen and click Unclog to start unclogging.
2. Go to the Service > Self-test > Valve/Pump
Abnormal
Self-test screen, click Valve 18 and check if it is
12 RBC RBC clogging.
working. If it is OK, please refer to 2.2.12.5 Aperture
counting
Clogging of the RBC Channel for operation.
3. Go to the Service > Maintenance > Maintain
screen and click RBC Channel Cleanser Soak.

Check the connection of the float sensor at the rear

Waste Waste container is
13 section of the machine; if the connection is OK, replace
overflow full.
the float sensor for waste overflow detection.

DIL-C expired. Expired reagent or insufficient residual amount.This

means that the reagent has gone out of date or the
Insufficient DIL-C. amount remaining is insufficient to support hydraulics
DIL-C not replaced. operations such as counting. Please following the
troubleshooting procedures below:
LYC-2 expired. Go to the Reagent Management > Setup screen,
Abnormal scan the barcode of the new reagent as shown in the
14 Insufficient LYC-1.
reagent alarm message, and then load the reagent to resolve
LYC-2 not replaced. the problem.

LYC-2 expired.

Insufficient LYC-1.

LYC-2 not replaced.

DIL-C running out. Check for the availability of the relevant reagent. If the
reagent is available, then check if the optocoupler of
15 No reagent LYC-1 running out or the reagent testing panel is on. If not, adjust the screw
air bubbles in inlet of the potentiometer and rotate counterclockwise to
tubing.

112
 6 Alarms and Solutions

Problem Problem Solution

Problem Description
No. Name
see if the optocoupler can be activated.
l If it works, drain the liquid from the tube and use a
multimeter to press the spot as shown in the
picture. Adjust the potentiometer and set the
voltage at 1.5V or so (the testing points are all
located in the left part of the picture, among which
Potentiometer A corresponds to Testing Point A,
and so on and so forth).

LYC-2 running out or

air bubbles in inlet
tubing.

l If it is not working, refer to 3.10 Reagent Testing

Panel Replacement to replace the reagent testing
panel.

Check if the right-side door is open. The door should

The right-side door is not press against the microswitch. If the door is
Door or open. properly closed, please replace the microswitch on the
16 cover open side door.
error. Open the optical shield cover, then close it again to
Optical assembly
check if the problem has been resolved; if not, replace
cover is open.
the optical microswitch.

113
 7 Maintenance Inventory

7 Maintenance Inventory

No. Material No. Material Name

1 20010121A WBC counting bath assembly

2 20010144A RBC counting bath assembly

3 68020006A 1104 syringe holder assembly

4 68020011A Main body of the syringe assembly (1104)

5 20010068A Sampling assembly

6 20010055A Negative-pressure chamber assembly

7 20010106A Front panel cover

8 22010018A Indicator light panel PCBA

9 22010022A Reagent presence testing PCBA

10 22010029A PCBA of the main control panel (Programmed) (1104)

11 22010036A Reagent sealing panel PCBA (Programmed)

12 68020001A Two-way valve assembly (S)

13 68020002A Three-way valve assembly (S)

14 68020003A Two-way valve assembly (L)

15 68020004A Three-way valve assembly (L)

16 23990005A Wire for the pinch valve

17 23990007A Wire for the location optocoupler

18 23990008A Wire for the liquid pump

19 23990015A Wire for sample absorption micro-switch

20 23990016A Wire for the temperature sensor

21 23990017A Wire for the self-control motor

22 23990018A Wire for the side door microswitch

23 24130015A Check valve for lyse

24 23990020A Belt pulley

25 23990043A Wire for dual fan

26 24020005A Stepping motor

114
 7 Maintenance Inventory

No. Material No. Material Name

27 24050004A Synchronous belt (TBN192MXL025)

28 24050002A Synchronous belt (TBN140MXL025)

29 31020001A LCD screen

30 68020008A Consumer screen (accessory)

31 68020009A Industrial screen (accessory)

32 31110003A Touch screen

33 32010006A Power

34 37010010A Control wire for AD input

35 37010021A Grounding wire for the rear panel

36 37020031A Valve control wire 1

37 37020033A Valve control wire 2

38 37020034A Control wire for the sample syringe

39 37020035A Control wire for the horizontal/vertical stepping motor

40 37020036A Control wire for the sampling optocoupler

41 37020037A Control wire for the syringe optocoupler

42 37020048A Control wire for the float sensor

43 37020040A Liquid pump control wire

44 37020042A Control wire for the temperature sensor

45 37020043A Control wire for heating of preheating bath

46 37020044A Control wire for heating of optical system

47 37020045A Control wire for the display screen

48 37020046A Control wire for the touch screen

49 37020047A Back-light control wire

50 37020049A Reagent control wires

51 37020055A Control wire for the front panel cover

52 37020060A Data singal wire for industrial screen

53 37020061A Backlight wire for industrial screen

54 37020063A 1104 Control wire for reagent sealing panel

55 53020001A Cross blind hinge

56 53090003A Guide shaft

57 53200001A Towing-chain connectors

58 53990001A Towing chain

115
 7 Maintenance Inventory

No. Material No. Material Name

59 56010063A Cleansing swabs

60 56010163A Open sample probe (Needle open)

61 60010012A Isolation chamber (one hole blocked)

62 60010012A Isolation chamber (no hole blocked)

63 60010046A 1104 Keyboard plate for sample absorption switch

64 60100024A 1104 series screen-printing housing

65 63010023A Double-layered tube (1000mm) for maintenance

66 63010024A EVA tube (1100mm) for maintenance

67 63010025A Slim No. 50 tube (1000mm) for maintenance

68 63010026A Wide No. 50 tube (850mm) for maintenance

69 63010027A No. 3603 tube (1500mm) for maintenance

70 63010028A MPF tube (1000mm) for maintenance

71 63010029A 1.5mm Teflon tube (1100mm) for maintenance

72 63010030A 1.0mm Teflon tube (1000mm) for maintenance

73 63010031A 2.4mm TPU rubber tube (300mm) for maintenance

74 63010032A 0.78mm silicone tube (50mm) for maintenance

75 63010033A Pharmed tube (50mm) for maintenance

76 63010034A 1.6mm thin silicone tube (40mm) for maintenance

77 63010035A 1.6mm thick silicone tube (80mm) for maintenance

78 63010036A 3.2mm silicone tube (200mm) for maintenance

79 63010037A 2.0mm (i.d.) Teflon tube (220mm) for maintenance

116
P/N: 65.02.0120A (5.0)