USER MANUAL

English

TEUS-00004-03-ING
May-2004
Esteemed customer,

Thank you for acquiring our photometer. Its features, we are sure, will make it a valued instrument
for your laboratory. Although it can be operated in a logical and simple way through its menu
options, we recommend you to read this manual carefully. It will help you for performing the
installation and the maintenance correctly, and will allow you to get the maximum benefit from its
many possibilities.
 TABLE OF CONTENTS
1. INTRODUCTION .................................................................................... 7
2. DESCRIPTION OF THE INSTRUMENT ............................................... 8
2.1. DESCRIPTION OF THE KEYBOARD ............................................................. 8
2.1.1. Numeric Keys ....................................................................................................... 8
2.1.2. Function keys ....................................................................................................... 8
2.1.3. Cursor keys .......................................................................................................... 9
2.2. DISPLAY DESCRIPTION ................................................................................ 9
2.3. DESCRIPTION OF THE COMMUNICATIONS ................................................ 9
2.3.1. Communication connector .................................................................................. 9
2.3.2. Configuration of the communication ................................................................. 9
2.3.3. Information transmitted ....................................................................................... 9
2.4. VERSIONS PROVIDING PRINTER ................................................................ 9
2.5. VERSION PROVIDING SIPPER ..................................................................... 9
2.5.1. Sipping cycle ........................................................................................................ 9
2.5.2. Elements of the SIPPER.................................................................................... 10
2.5.3. Programmable parameters ................................................................................ 10
2.6. MEASUREMENTS WITH COMMON CUVETTES OR TUBES .................... 10
2.7. MODE OF OPERATION BY MENUS ............................................................. 10
2.7.1. Viewing a menu on the display and selecting an option ................................ 11
2.7.2. Selecting a job. Selection path. ....................................................................... 11
2.7.3. Exiting from a job ............................................................................................... 12
2.8. LOCATIONS FOR PROGRAMMING TESTS ................................................ 12
2.9. UNITS ............................................................................................................ 12
2.10. FILTERS ...................................................................................................... 12
2.11. INPUTTING DATA ....................................................................................... 12
2.11.1. Alphanumeric input .......................................................................................... 12
2.11.2. Numeric input ................................................................................................... 12
2.11.3. Selection by cursors ........................................................................................ 13
2.12. INTERNAL QUALITY CONTROL................................................................ 13
3. GENERAL METHOD OF OPERATION............................................... 14
3.1. STARTING OPERATION. THE MAIN MENU. ............................................... 14
3.2. ABSORBANCES ........................................................................................... 15
3.3. CONCENTRATIONS ..................................................................................... 16
3.3.1. Temporary modification of parameters ............................................................ 16
3.3.2. Heading listings (only versions with printer) .................................................. 16
3.3.3. Measurement process with base line .............................................................. 16
3.3.3.1. Process with reagent blank ........................................................................... 16
3.3.3.2. Process without reagent blank ..................................................................... 17
3.3.4. Measurement process with reagent blank...................................................... 17
3.3.5. Measurement procedures ................................................................................ 17
3.3.5.1. End point, increasing ..................................................................................... 17
3.3.5.2. End point, decreasing .................................................................................... 18
3.3.5.3. Delta kinetics ................................................................................................... 18
3.3.5.4. Differential mode ............................................................................................ 18
3.3.5.5. Fixed time ........................................................................................................ 18
3.3.5.6. Multistandard................................................................................................... 19
3.3.5.7. Multiple-test procedures ............................................................................... 19
3.3.6. Resolution in measurements, display, printer and calculations ................... 21
3.3.8. Error messages .................................................................................................. 21
 3.3.7. Ending a measurement cycle ............................................................................ 21
3.4. PROGRAMMING ........................................................................................... 22
3.4.1. Programming tests ............................................................................................. 22
3.4.1.1. Creating a Test ................................................................................................ 22
3.4.1.1.1. General procedure ...................................................................................... 22
3.4.1.1.2. How to program parameters ....................................................................... 22
3.4.1.1.3. Exit menu ...................................................................................................... 26
3.4.1.2. Reviewing a Test ............................................................................................ 26
3.4.1.3. Delete a test .................................................................................................... 26
3.4.1.4. Copy a test ....................................................................................................... 26
3.4.1.5. Move a test ...................................................................................................... 27
3.4.2. Personalization of the photometer .................................................................. 27
3.4.2.1. Language ......................................................................................................... 27
3.4.2.2. Laboratory name (only in versions with printer) ......................................... 27
3.4.2.3. Screen contrast ............................................................................................... 28
3.4.2.4. Selection of the wash mode (only in versions with sipper) ....................... 28
3.4.2.5. List headings (only in versions with printer) ............................................... 28
3.4.2.6. Adjusting the printer (only in versions with printer) ................................... 28
3.4.2.7. Patient code .................................................................................................... 28
3.4.3. Programming the filters table ........................................................................... 28
3.4.4. Programming units ............................................................................................. 29
3.4.5. Programming communications ......................................................................... 29
3.4.5.1. Configuration of RS-232-C ............................................................................. 29
3.4.5.2. Default parameters ......................................................................................... 29
3.5. LISTING ......................................................................................................... 29
3.5.1. Listing the display .............................................................................................. 30
3.5.1.1. Listing tests ..................................................................................................... 30
3.5.1.2. Listing units ..................................................................................................... 30
3.5.2. Listing in the printer (only in versions with printer) ...................................... 30
3.5.2.1. Listing tests ..................................................................................................... 30
3.5.2.1.1. Listing of the tests names ........................................................................... 30
3.5.2.1.2. Listings of tests parameters ....................................................................... 30
3.5.2.2. Listing the personalization parameters ....................................................... 30
3.5.2.3. Listing the filters table ................................................................................... 30
3.5.2.4. Listing units ..................................................................................................... 31
3.5.2.5. Listing the communication parameters ........................................................ 31
3.5.2.6. Listing the pump parameters ......................................................................... 31
3.6. OTHER OPTIONS ......................................................................................... 31
3.6.1. Service ................................................................................................................ 31
3.6.2. Check lamp ......................................................................................................... 31
3.6.3. Adjust of the peristaltic pump (only in versions with sipper)........................ 31
3.6.3.1. Checking adjust of the sample volume ........................................................ 32
3.6.3.2. Manual adjust .................................................................................................. 32
3.6.3.2.1. Sample volume ............................................................................................. 32
3.6.3.2.2. Sample positioning ...................................................................................... 32
3.6.3.2.3. Pump delay time ........................................................................................... 32
3.6.3.3. Automatic adjust .............................................................................................. 33
3.6.3.3.1. Sample volume ............................................................................................. 33
3.6.3.3.2. Sample positioning ...................................................................................... 33
3.6.4. Load a new program .......................................................................................... 33
3.6.5. Delete the concentration values ..................................................................... 33
APPENDIX I: MENUS TREE. VERSION WITH PRINTER AND SIPPER. ................................................. 34
APPENDIX I: MENUS TREE. VERSION WITH PRINTER AND WITHOUT SIPPER. ................................ 35
APPENDIX I: MENUS TREE. VERSION WITHOUT PRINTER AND WITHOUT SIPPER. ......................... 36
1. INTRODUCTION
This photometer has been designed for use in "in vitro" The functionality of this photometer is completed by a series
diagnosis, and should therefore only be used by of automatic calculation modes that cover most of the
professionals. Its function is based on measuring the possibilities of photometric analysis: end point, differential
analyte concentration present in a sample by means of mode, fixed time and kinetics. The calculations also make
absorbency measurement, after application of one or several it possible to use standard or factor and bichromatics.
reagents.
The instrument is manufactured in three versions providing
Despite the large number and the complexity of tasks that different accesories:
the photometer is capable of performing, its operation is
extraordinarily simple through the menu system and the - Version with printer and sipper
underlying logic of its interface, so that an easy-to-assimilate - Version with printer and without sipper
dialogue is established between the user and the instrument. - Version without printer and without sipper

Besides great reliability in the optics, this instrument offers

versatility in the measurement devices: flow micro-cuvette,
common cuvettes or micro-cuvettes, and tubes. The cuvettes WARNING
are thermoregulated in a rapid and precise manner by
means of the Peltier system, without requiring connections The photometer has been designed to be
for circulating water. exclusively used for professional purpose. The
user must have a suitable training to work in
Its sophisticated software allows the programming of up to clinical laboratories and to use an in vitro
21 different parameters that define a set test. It can store up diagnostic photometer. This manual should be
to 75 distinct tests. On the other hand, programming is carefully read before starting to operate, paying
simple and is facilitated by the existence of commands for attention to all the warning and cautions written
“copy” and “move”. Besides the tests, many other functions in it.
of the instrument can be programmed, such as: laboratory
name, language, new filters, wash mode, contents of the
print header, communications, etc.

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User Manual

2. DESCRIPTION OF THE INSTRUMENT

The main function of the photometer is the measurement of numerical value being input (before being confirmed, usually
analyte concentrations present in a sample. pressing ENTER), deleting the whole number.

The photometer sucks the sample up through a teflon tube. 2.1.2. Function keys
To accomplish this a peristaltic pump is employed that is
controlled by the microprocessor. When the sample has The function keys are those which allow the general operation
been positioned in the flow tray, the program will maintain of the program jobs or activate some general functions when
the programmed temperature thermostatically, if the using the photometer.
technique requires this. A bundle of monochromatic light is
passed through the sample to be measured and enters a ENTER/YES Is used to confirm the data input through the
photodiode which converts the light to a current. numeric keyboard, or to answer YES to the questions the
program may pose. Other specific uses are explained
Absorbency is a relative measurement, i.e. two throughout the manual.
measurements are made, one of the sample and the other,
normally of distilled water, is a reference measurement. ESC/NO Is used to quit one job in order to go to the
preceeding one in the program, such as to pass from one
Absorbency is calculated using the following relation: menu to the previous one, or to answer NO to the questions
the program may pose. Other specific uses are explained
Reference current throughout the manual.
Absorbency = log
Sample current
END This key lets you quit most of the jobs in the apparatus,
going to the main menu. It is useful to avoid going through
The concentration of the analyte of the sample will be the intermediate menus and to abandon long processes that
proportional to its measured absorbency. you do not desire but that you have started by error.

2.1. DESCRIPTION OF THE KEYBOARD WASH (only in versions with sipper) This key lets you
operate the pump independently of the program, allowing
2.1.1. Numeric Keys you to perform washes of the tubing and of the cuvette at any
time. This key may operate continuously or on a fixed
The keys 0 to 9, together with the decimal point and the C volume mode.
key, constitute the numeric keyboard. Keys 0 to 9 and the
decimal point serve for inputting numerical data when the PUMP (only in versions with sipper) This key is used to start
program requires it. The C key is used to correct the the SIPPING CYCLE, aspirating the sample to be measured.
The key has an indicator which lights up every time the pump
is ready to start a new cycle (it is not necessary to wait for

1 3

Figure 2.1 Keyboard

8
the display nor the printer in order to sip the next sample). In order to have a right communication between PC and the
photometer, all these parameters must be the same in both
2.1.3. Cursor keys apparatus
The Default values are:
There are four cursors on the keyboard, two called VERTI-
CAL CURSORS (CURSOR UP and CURSOR DOWN) and Bauds: 9600
two called HORIZONTAL CURSORS (CURSOR RIGHT and Timeout: 0
CURSOR LEFT). These cursors have diverse functions in Terminal: 0
2
the program according to the job the photometer is performing,
so they will be explained throughout the manual when 2.3.3. Information transmitted
necessary.
With this PC program two different types of information can
2.2. DISPLAY DESCRIPTION be transmitted.

The display is made of liquid crystal (LCD) and consists of 1- The memorised values of concentration measured. These
240 x 64 pixels. The distribution of text and data on it will results are applied to perform the final report.
depend on the job being performed.
2- The photometer program can be changed from the PC.
2.3. DESCRIPTION OF THE COMMUNICATIONS
2.4. VERSIONS PROVIDING PRINTER
The photometer results can be transmitted to a PC(Personal
Computer) through a communications cable. A PC program Some versions of the photometer include a thermo-printer,
is supplied with the photometer, allowing the management allowing text to be printed rapidly and silently. The printer
of all communications and data received between the PC works bi-directionally, optimizing the path of the head and
and the photometer. consequently the print time. Paper advance is performed
rapidly by means of an independent motor.
2.3.1. Communication connector
Text is printed in forty columns, producing an uncrowded
In the rear part of the apparatus, you can see the connector appearance. The print format will depend on each job.
(37) for the computer connection, in a box marked COM1.
The connector is a RS-232 with the following line connections:
2.5. VERSION PROVIDING SIPPER
All control lines and electrical levels of the serial connector
are compatible with the standard E.I.A. RS-232. The SIPPER has the duty of handling the samples to be
measured. It is one of the most delicate parts of the
2.3.2. Configuration of the communication instrument and must be treated with the utmost care. This
section explains the functioning of this circuit, its constituent
In the serial port the following parameters can be configured: elements and the parameters that control it.
-Baud rate, is the transmission velocity
-Timeout, is the resent time
-Terminal number

SERIAL PORT PIN SIGNAL

1 NC
5 1 2 RXD
3 TXD
4 NC
9 6 5 GND

Figure 2.2 Serial channel connector

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User Manual

2.5.1. Sipping cycle 2.5.3. Programmable parameters

This cycle involves aspirating the sample to be measured Three parameters control the sipper functioning and they must be
and transporting it to the flow cuvette. It consists of three programmed to obtain the expected performance characteristics.
consecutive steps: a) SAMPLE VOLUME. It is a number that allows to adjust
the volume of sample that will be sipped.
SIPPING: The sample is aspirated in by the sip tubing up to
the programmed volume. b) PUMP DELAY. It is the number of seconds the pump will
wait from the moment the sipping finishes up to the
PUMP DELAY: Waiting period from the moment the sipping moment the pump activates again to position the sample.
finishes. The tube or vessel with the sample must be
pulled out the sip tubing during this time. c) POSITIONING. This is also a number that determines the
transportation of the sample to the cuvette, ensuring that
POSITIONING: The pump activates again transporting the it is suitably positioned to be read.
sample to the cuvette and positioning it suitably for
its measurement. These parameters depend on the length, the diameter and
the aging of the tubing, and must be controlled and adjusted
The measured samples are transported to the waste bottle periodically.
when new samples are sipped or a wash is performed.

2.5.2. Elements of the SIPPER 2.6. MEASUREMENTS WITH COMMON

(All following numbers are referenced to the figures from CUVETTES OR TUBES
Installation and maintenance manual)
Common cuvettes may be used for measurements in all
a) A SIP TUBING (17) made of teflon which, from the cuvette versions of this instrument. Cuvettes can be macro, semi-
passes out of the apparatus through a metallic guiding micro or micro, made of glass or plastic, taking into account
tube, to which it is attached by a silicone connector. This that some of the latter are not suited for reactions at a strictly
tube has a pre-defined length and must be of appropriate controlled temperature, since the liquid is far away from the
quality, so original tubes must always be used. It must walls of the cuvette-holder and the plastic acts as insulation.
have neither folds or cracks; if so, it must be replaced.
The optical system of this photometer is designed to
b) An INLET ADAPTER for attaching the teflon tubing to the perform measurements with test tubes with a diameter of 12
cuvette. mm. Their height must not exceed 75 mm since otherwise
the access door to the cuvettes compartment could not be
c) The FLOW CUVETTE (16), where measurements take closed.
place. It must be the original cuvette, for which the
optical system has been designed and adjusted. In case To read with test tubes (figure 2.6) insert the test tubes
of breakage or loss, contact the Technical Assistance adapter (7) in the cuvette-holder (42).
Service in order to acquire a new one with the appropiate
characteristics. To prevent breakage, keep it always In versions providing sipper, pull out the flow cuvette (16) and
inside the cuvette holder (42) or in the cuvette lodgement insert it in the lodgement for that purpose (46). The measure-
(46) that is located to the left for that purpose. ments with common cuvettes, as with test tubes, are
performed by pressing ENTER.
d) The OUTLET ADAPTER to which is connected the tubing
of the peristaltic pump.
ATTENTION
e) The TUBING OF THE PERISTALTIC PUMP (15) which
The photometer has been adjusted for 10 mm square
connects the flow cuvette with the waste outlet (44).
cuvettes, so the absolute values of absorbances
read using test tubes will not coincide with the
f) The PERISTALTIC PUMP (47), which is the mechanism that
measurements of the same samples performed with
together with the tubing, performs the job of sipping and
10 mm cuvettes. Because of this, it is advisable to
transporting the samples to be measured. It is operated by
use tubes only in tests with a STANDARD.
a low-voltage stepper motor, with a resolution of 1/100
revolution, guaranteeing excellent functioning and high
precision. 2.7. MODE OF OPERATION BY MENUS

One of the main features of this photometer is to make the

high number of jobs it can perform compatible with simplicity
of operation. This apparatus uses a method that is becoming
10
more and more familiar to the user, due to the diffusion of 2.7.2. Selecting a job. Selection path.
personal computers, ie. the use of menus. A menu is a set
of options grouped together in the form of a list, and On selecting an option of the MAIN MENU, another menu
numbered. The following list is an example of a menu, in this appears on the display. This is a SECOND LEVEL menu.
case corresponding to the so-called MAIN MENU. On selecting an option of the second level menu, another
menu (THIRD LEVEL) may appear on the display.
1 ABSORBANCES Sometimes, there exist more levels of linked menus. In all
2 CONCENTRATIONS cases, the options are selected simply by pressing the
3 PROGRAMMING option number.
4 LIST
5 OTHER OPTIONS Continuing with the example of the MAIN MENU, if we select
option 3 (PROGRAMMING), the following menu will appear:
2.7.1. Viewing a menu on the display and
selecting an option 1 TESTS
2 PERSONALIZE
The photometer only displays four lines. If the menu has four 3 FILTERS TABLE
or less options, they all appear on the display, for example: < 4 UNITS

1 SERVICE and if we then select option 1 (TESTS), the following menu

2 CHECK LAMP will appear:
3 ADJUST PUMP
4 COMMUNICATIONS 1 CREATE NEW TEST
2 REVIEW
If the menu has more than four options, only the first four 3 DELETE
appear in the display, with a marker in the fourth one, < 4 COPY
indicating that there is more text below. For example:

1 CREATE NEW TEST At the end of selecting several options, a state is reached
2 REVIEW where the apparatus executes a job. For example, if we
3 DELETE select 1 (CREATE NEW TEST) from the previous menu, the
< 4 COPY following will be displayed:

In order to see the options below the last line, press the
CURSOR DOWN, and the list will move down one line, CREATE TEST
leaving: LOCATION ........ = _
1st available .... 25
< 2 REVIEW
3 DELETE
4 COPY
5 MOVE indicating that we are in the task of CREATE A NEW TEST.

Note that the same marker appears now in the first row, To start this task, it has been necessary to select three
indicating that there is more text above it. Press the consecutive options. The expression:
CURSOR UP to move up one line.
PROGRAMMING/TESTS/CREATE NEW TEST
In the case of a long list, when there is text above and below
the first and fourth rows of the display, respectively, these formed by the selected options, is called a PATH, and from
markers appear in both rows. this point on and throughout this manual, it will be the way
of indicating the set of consecutive options in the linked
Using the CURSORS UP and DOWN, all the options in any menus that have to be selected in order to reach the task
list can be viewed. being explained.

A menu option is selected by pressing the numeric key Sometimes, suspension points will appear in the middle of
corresponding to the number of the desired option. It is not a path (OPTION/.../OPTION), meaning that it is necessary
necessary to press ENTER. to input a parameter between both options.

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User Manual

programming a location), is made either by means of the

ATTENTION WAVELENGHT of the filter, or by its position in the wheel.
Throughout this manual,the menus are shown with In order that the photometer can use the appropriate filter,
all their options, whether or not all are seen on the it is necessary to programme in which position each one is
display when they are called up. located (section 3.4.3.) in the wheel. The positions of the
filters the apparatus is equiped with are programmed in the
factory and are shown in table 3.3.

2.7.3. Exiting from a job 2.11. INPUTTING DATA

The general way of exiting from a job is by using the ESC
Three different types of data may be input into the photometer:
key or the END key. The former allows you to return to the
alphanumeric, numeric and selection by cursors.
previous program step (generally a menu). The END key
returns directly to the MAIN MENU. Sometimes, exiting a
job is not as direct because of operational reasons and the 2.11.1. Alphanumeric input
detailed procedure will be described where applicable.a
razones operativas. El procedimiento detallado será descri- When the program requires alphanumeric text input, a row
to adecuadamente en cada caso. of hyphens appears on the display, as many as the
maximum characters to input. If there is an already existing
text, it will appear, with hyphens in the free positions to the
2.8. LOCATIONS FOR PROGRAMMING TESTS
right in which text has not yet been input.
There are 75 LOCATIONS, numbered from 0 to 74, for
In order to input alphanumeric data, proceed as follows:
programming up to 75 different analysis tests. Each location
contains a certain number of parameters which will depend
a) Press CURSOR RIGHT, and the writing cursor will appear
on the MEASUREMENT PROCEDURE selected (section
in the first position of the text.
3.3.5.).
b) Using the VERTICAL (UP and DOWN) CURSORS,
letters, numbers and symbols can be selected in a
Each location can contain a test with any measurement
rotating way. Select the desired character on the display
procedure and can be permanently stored. The contents of
above the writing cursor.
a location may also be deleted, copied or moved from one
c) Go on to the next position by means of the CURSOR
number to another (section 3.4.1.).
RIGHT and select again an alphanumeric character using
the VERTICAL CURSORS. Repeat this step as many
times as necessary to complete the text (whithin the
ATTENTION limits of the free hyphens).
Some locations may be pre-programmed with specific d) Using both HORIZONTAL CURSORS, the text can be
TESTS on leaving the factory scrolled from one end to the other, and the characters in
each position can be modified as many times as required.
e) If you want to delete all the text, press C. In case you want
to abandon the process, press ESC and the text will be
disregarded.
2.9. UNITS f) To confirm the text (and quit the process) press ENTER.
The text will be permanently stored.
The concentration values obtained on executing the different
tests must be expressed in their corresponding units. In In alphanumeric input, the CURSORS have a repeating
order that the user is not at all restricted, the instrument function. If they are held down for a period longer than one
permits the programming of the units that are desired second, they advance automatically.
(section 3.4.4.). Each unit is formed by a text of maximum
eight characters and is stored in a table with up to 50 2.11.2. Numeric input
positions. The most common units are pre-programmed in
factory and are shown in table 3.2.
It is used when parameter values are required by the
program. To input numeric data, the NUMERIC keys and
2.10. FILTERS DECIMAL POINT of the keyboard are used. The program
limits in each case the number of digits to input.
The filter wheel of the optic system allows a total of up to nine
filters. Each filter is mounted in a filter holder, which can be The writing cursor appears in the first position and goes to
placed in any of the nine positions in the wheel. The user can the next position on inputting a number (or decimal point).
change the position of the filters, or add more if necessary.
If you want to correct the number before confirmation, press
Reference to filters throughout the program (for example, on

12
C and the complete number will be deleted. 2.12. INTERNAL QUALITY CONTROL
To confirm the number (and quit the process) press ENTER.
The number will be permanently stored. All clinical analysis laboratories should have internal quality
control procedures so that they may verify the correct
Often, the following titles appear on the display: functioning of each measurement procedure in use, and for
the validation or rejection of measurements made.
Actual value: Which is the current value of the parameter
(previous data input or default value). Since the photometer is a manual instrument, there is no
New value: The one which is input and that will be stored on special option for effecting such control. Thus, it becomes
pressing ENTER. the responsibility of laboratory personnel to carry out an
internal quality control programme, in which intercalated
control samples would be measured daily along with other
2.11.3. Selection by cursors samples for later comparison with their respective expected
values. In this way it would be possible to detect the
In some cases, the user must choose an item among a pre- presence of occasional anomalies in measurement
defined set of them. This can be achieved by means of the procedures. It is recommended that one employs control
HORIZONTAL or VERTICAL CURSORS according to the materials titrated by Biosystems.
case. By pressing the CURSORS, the different options will
appear on the display. The manner of confirming them is
usually by pressing ENTER, although it may differ from case
to case, and will be explained throughout the manual.

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User manual

3. GENERAL METHOD OF OPERATION

3.1. STARTING OPERATION. THE MAIN MENU. the instrument can be operated by pressing ENTER when
other errors are detected in the selftest. However, anomalous
When the photometer is connected, the following appears functioning may be observed. It is recommended to call the
on the display: technical assistance service in case any selftest caution
message appears on the display.

SELFTEST When errors are not detected in the selftest process, the
following display appears in about 5 seconds:

in process

<model> Ver n.m

The instrument checks if some kind of error or alteration has Ready to work
happened in any of the following informations, and displays Press ENTER
caution messages only in case of alteration. serial number:<xxxxx>

Information checked Caution message <model> is the reference to the apparatus model
Ver n.m is the version of the program installed.
Serial number: is the serial number of the apparatus
Program
ROM ERROR Press ENTER and, in versions providing printer, the display
will show:

New date ... =

Personalization
PERSONALIZE CHECKSUM
ERROR
The date can then be input. This date will be printed heading
Press ENTER most of the printed information.

To input date use the numeric keyboard. Two ciphers are

Adjustment required per day, month and year (input 0 first for one-cipher
ADJUSTMENT CHECKSUM days or months). Bars will automatically appear. Press
ERROR ENTER to go to the next menu.

Press ENTER Example:

Locations contents New date ... = 03/09/94

LOCATION CHECKSUM
ERROR
In case you do not desire to print the date press ESC/NO
Press ENTER to continue the instrument operation.

The MAIN MENU will then appear:

If a program error is detected, the instrument functioning is
blocked and it is not possible to operate it. On the contrary, 1 ABSORBANCES
2 CONCENTRATIONS
3 PROGRAMMING
4 LIST
5 OTHER OPTIONS

14
Seleccionar una de estas opciones, dependiendo del tra- c) Sample volume (only in versions with sipper). Input the
bajo que quiera realizarse (ver Apéndice I): volume (in mL) of sample to be sipped (section
3.4.1.1.2.m)
Select one of these options depending on the task you want d) Stabilization time (only in versions with sipper). Input the
to perform (see appendix I): time (in seconds) you want to elapse between the end of
the SIPPING CYCLE and the measurement (section
1. ABSORBANCES: To measure absorbances. 3.4.1.1.2.i).
e) Temperature. Input the temperature at which it is desired
2.CONCENTRATIONS: To measure concentrations to thermostat the cuvette or “0” for no thermostating
according to a predefined test which has been programmed (section 3.4.1.1.2.p).
in a location.
Once the temperature have been selected, the display will
3.PROGRAMMING: To programme tests and other show:
parameters which will control different functions of the
instrument.

4.LIST: To obtain lists of the programmed parameters THERMOSTATING

through the display or the printer (the latter, only in Wait X minutes
versions of the instrument providing printer).

5.OTHER OPTIONS: To be used by the technical service,

check the lamp, adjust the pump parameters (only in This display will not appear when the instrument has been
version with sipper) and execute communications with previously operating at the same temperature and the
outer devices. cuvette holder is already thermostated, nor when
thermostation is not required. If you do not want to wait for
3.2. ABSORBANCES thermostating, press ENTER to continue the instrument
operation.
PATH: ABSORBANCES
Nextly, the display shows data about the selected test
With this option, it is possible to directly read absorbances, (mono/bichromatic, filters) and requires:
without having to call a location. The following menu appears:
Insert BASE LINE
1 MONOCHROMATIC
2 BICHROMATIC By inserting water or a blank and pressing ENTER (PUMP
in versions with sipper), the photometer will adjust the
PATH: ABSORBANCES/MONOCHROMATIC absorbance to zero. The display will require:
ABSORBANCES/BICHROMATIC
Insert SAMPLE
In MONOCHROMATIC, the instrument measures
absorbances at a selected wavelength and temperature. In Samples can then be sequentially measured by inserting
BICHROMATIC, the photometer will measure the difference the cuvette and pressing ENTER or, in versions with sipper,
between absorbances read at two wavelengths. The diagram by pressing PUMP. The absorbance value will appear in the
in appendix II summarizes the whole process of measuring display and the next sample will be required.
absorbances.
It is possible to re-start the measurement process or to
By selecting any of monochromatic or bichromatic, the measure against a new base line by pressing ESC. Once
instrument requieres, through the display, values for several the measurements have been finished, you can go again to
parameters which will appear sequentially. Select, or input the main menu by pressing END.
by means of the numeric keyboard, the desired values and
confirm them by pressing ENTER (section 2.11.). In versions of the instrument providing a printer, the following
will be automatically printed:
a) Reading filter. Input the number corresponding to the
wavelength at which it is desired to make the - MONO/BICHROMATIC ABSORBANCE
measurements (section 3.4.1.1.2.g). In BICHROMATIC
mode, it refers to the main wavelenght. - A heading with the selected data for the measuring
parameters.
b) Reference filter (only in BICHROMATIC mode). Input the
number corresponding to the secondary or reference - Zero absorbance value
wavelength (section 3.4.1.1.2.f).

15
User manual

- Samples consecutively numbered and their respective 3.3.2. Heading listings (only versions with printer)
absorbance values.
PATH: CONCENTRATIONS/.../LIST
3.3. CONCENTRATIONS
With this option, you can print again the heading of the Test
PATH: CONCENTRATIONS for the retrieved location. This is specially useful when
parameters have been modified in MODIFY PARAMETERS
This option allows the calculation of concentrations by
using any of the TESTS previously programmed and stored
in the 75 available LOCATIONS. On selecting this option, 3.3.3. Measurement process with base line
the following display appears:
PATH: CONCENTRATIONS/.../BASE LINE

CONCENTRATIONS This option allows to adjust the absorbance to zero usually

LOCATION ........ = _ against water. The process followed by the program is
different depending on the procedure selected for the retreived
Test.

Input the number of the location desired and press ENTER. 3.3.3.1. Process with reagent blank
In versions providing printer, the instrument will print the In the following group of procedures:
name of the test, the location number and the calculation
mode, as well as the values of the different parameters only - End point or multistandard, increasing slope
if this option has been selected in LIST HEADING (section - Differential mode
3.4.2.5.).
The absorbance is generally adjusted to ZERO against the
Once the location number has been input, two different REAGENT BLANK (section 3.3.4), but sometimes it is
menus may appear on the display, depending on the convenient to know the absorbance of the reagent blank.
calculation mode and on the reaction slope of the selected This value may be obtained by selecting BASE LINE in the
Test. menu. Upon selection, the display will show:
a) In measurement procedures where the reagent blank is
low and it is usual to adjust to ZERO with it, such as END
POINT and MULTISTANDARD (increasing) or
DIFFERENTIAL MODE, the following menu will appear: THERMOSTATING
Wait X minutes
1 MODIFY PARAMETERS
2 BASE LINE
3 REAGENT BLANK
4 LIST This display will not appear when the instrument has been
previously operating at the same temperature and the
b) In measurement procedures where the reagent blank is cuvette holder is already thermostated, nor when
not used (FIXED TIME, KINETICS) or has a high value thermostatation is not required.
(DECREASING reactions) and it is usual to adjust to ZERO
with water, the following menu will appear: Nextly, the display requires:
1 MODIFY PARAMETERS
2 BASE LINE Insert
3 LIST

3.3.1. Temporary modification of parameters BASE LINE

PATH: CONCENTRATIONS/.../MODIFY PARAMETERS

By inserting water or other liquid and pressing ENTER
With this option you can modify the programmed values of (PUMP in versions with sipper), the photometer will adjust
the different parameters in the retrieved Test. The modification the absorbance to zero and the display will require:
will be temporary and will be abandoned at the moment of
retrieving another location.
16
 Insert Insert

REAGENT BLANK REAGENT BLANK

Insert the reagent blank and press ENTER (PUMP in

The reagent blank must then be inserted and, after pressing versions with sipper). The photometer will set the absorbance
ENTER (PUMP in versions with sipper), the photometer will re- to zero against this blank and the display will show:
set the absorbance to zero against this blank and the display will
show (example):
ZERO....... = 0.000

BLANK ABS. = 0.097 Insert SAMPLE

ZERO. . . . . . = 0.000

Insert SAMPLE 3.3.5. Measurement procedures

Once the absorbance has been adjusted to zero against a

base line or a reagent blank, the measurement process
3.3.3.2. Process without reagent blank
continues depending on the procedure that has been
programmed in the retreived location. Diagrams A, B, C and
In the following group of procedures:
D in appendix III show the different steps that are followed
in each procedure. The measurement process depends
- End point and multistandard, decreasing slope
basically on the CALCULATION MODE and on the REAC-
- Delta kinetics
TION SLOPE that have been programmed for the particular
- Fixed time
Test (section 3.4.1.1.2.).
- Multi-kinetics
- Multi-fixed time
The following abbreviations are used in the formulae:
The absorbance is generally not adjusted to zero against a
Cs = Concentration of the sample
reagent blank. Two cases have to be distinguished:
Cst = Concentration of the standard
As = Absorbance of the sample
a) In DELTA KINETICS and FIXED TIME, the reagent blank
Ast = Absorbance of the standard
is not used and adjustement to zero is against a base line
Arb = Absorbance of the reagent blank
(generally water).
Asb = Absorbance of the sample blank
Astb = Absorbance of the standard blank
b) In END POINT and MULTISTANDARD (decreasing slope),
Ast1 = Absorbance of the standard at time T1
after adjustment to zero against the base line (water), the
Ast2 = Absorbance of the standard at time T2
display requires the REAGENT BLANK. Once this blank
As1 = Absorbance of the sample at time T1
has been inserted, the photometer measures its absorbance
As2 = Absorbance of the sample at time T2
and shows its value on the display, but does not re-set to
N = Number of intervals
zero.
t = Interval time (in seconds)
Ds = Delta of sample
The process followed in both cases is similar to that
Dst = Delta of standard
described in section 3.3.3.1. (see also diagrams A, B and
F = Factor
D in appendix III).
3.3.5.1. End point, increasing
3.3.4. Measurement process with reagent blank
The absorbance of each sample is read against the
PATH: CONCENTRATIONS/.../REAGENT BLANK
REAGENT BLANK (diagram A, appendix III), and the
concentration is calculated by multiplying the absorbance
This option (see diagrams A and C in appendix III) appears
by a factor or with reference to the standard.
only when the retrieved Test is programmed with one of the
procedures: END POINTand MULTISTANDARD (increasing)
Factor: Cs = As x F
or DIFFERENTIAL MODE and allows you to adjust the
absorbance to zero against the REAGENT BLANK. Upon
Cst
selection, the display may show the indication of
Standard: Cs = As x
THERMOSTATING, and then requires:
Ast
17
User manual

BLANK, and the concentration is calculated by multiplying display requests: LIST DELTA?, offering the possibility of
the absorbance by a factor or with reference to the standard. reviewing the delta values (diagram B, appendix III). Answer
ENTER/YES to print them or ESC/NO and the display will
Factor: Cs = As x F request: DELETE DELTA?. You can eliminate one or
several deltas by pressing ENTER/YES and following the
Cst procedure:
Standard: Cs = As x
Ast a) Select the delta you wish to delete by using the VERTI-
CAL CURSORS.
The display shows the measured absorbances and the b) Press ENTER/YES to delete the delta on the display, or
calculated concentration of each sample. C to retrieve a previously deleted delta.
c) Once all the desired deltas have been deleted (or retrieved
In versions providing printer, the samples are consecutively if previously deleted), press ESC. The concentration will
numbered and their respective absorbance and concentration be re-calculated using the selected deltas and printed
are printed. again.
d) You may then list and select again deltas (pressing
3.3.5.2. End point, decreasing ENTER/YES) or go to the next sample by pressing ESC/
NO.
The absorbance of the reagent blank and of each sample is
read against the BASE LINE, and the concentration is 3.3.5.4. Differential mode
calculated by multiplying the difference between the
absorbances of the REAGENT BLANK and the sample by Two tubes are required per sample, one for the reaction of
a factor or with reference to the standard. the sample blank, and another for the overall reaction. After
adjusting to zero with the REAGENT BLANK, the instrument
Factor: Cs = (Arb - As) x F sequentially requires the sample blank and the corresponding
sample, in order to perform the measurements (diagram C,
Cst appendix III). The concentration of the sample is calculated
Standard: Cs = (Arb - As) x by substracting the absorbance of the sample blank from
(Arb - Ast) the absorbance of the sample, and multiplying by a factor
or with reference to the standard.
The display and printer provide the same data than in end
point, increasing. Factor: Cs = (As - Asb) x F

3.3.5.3. Delta kinetics Cst

Standard: Cs = (As - Asb) x
In each sample, a first measurement of absorbance is made (Ast - Astb)
against the BASE LINE after a programmed incubation
period. Next, new measurements are made at defined The display shows the measured absorbances and the
intervals of time. The concentration is calculated by calculated concentration of each sample.
multiplying the mean of the increase (or decrease) in
absorbance per minute by a factor or with reference to the In versions providing printer, the samples are consecutively
standard. numbered and their respective absorbances and
concentration are printed.
Σ∆s 60
Factor: Cs = x xF 3.3.5.5. Fixed time
N t
A first absorbance measurement (A1) is made for each
ΣDs sample after a programmed incubation period (T1), and a
Standard: Cs = x Cst second measurement (A2) is performed after a defined
Σ∆st interval (T2) (diagram D, appendix III). The concentration is
calculated by multiplying the absorbance difference by a
The measured absorbances are sequentally displayed, as factor or with reference to the standard.
well as the mean delta and the calculated concentration of
each sample. Factor: Cs = (As2 - As1) x F

In versions providing printer, the samples are consecutively Cst

numbered and their respective first absorbance Standard: Cs = (As2 - As1) x
measurement, mean delta and concentration are printed. (Ast2 - Ast1)
At the end of the measurement process of each sample, the
18
The display shows the measured absorbances and the a) PRINT CURVE: Only in versions with printer. The stan-
calculated concentration of each sample. dard curve is printed in the selected format (axis and
calculation mode). The replicates and the mean for each
In versions providing printer, the samples are consecutively standard are shown.
numbered and their respective absorbances and b) LIST STANDARDS: The values of the read absorbances,
concentration are printed. their means, and the programmed concentration values
are printed. In versions without printer, the absorbance
3.3.5.6. Multistandard values are shown in the display and you may pass from
a standard to the next one using the VERTICAL
The absorbance of each standard and of each sample is CURSORS.
read against the REAGENT BLANK (increasing curve) or c) DELETE REPLICATES: You can eliminate one or several
against the BASE LINE (decreasing curve). The concentration replicates by selecting this option and following the
is calculated by interpolation in a standard curve. procedure:
c1) Select the replicate you wish to delete using the
After reading the reagent blank, the instrument consecutively VERTICAL CURSORS.
requires the standards. A stored curve previously read may c2) Press 1 to delete the replicate on the display, or 2 to
also be used. retrieve a previously deleted replicate.
c3) Once the desired replicates have been deleted (or
The display shows the absorbances obtained for each retrieved), press ESC or ENTER. The new standard curve
standard. After the last standard has been read, the will be checked for possible abnormal progression of
instrument checks the standard curve and shows the values.
caution message ABNORMAL CURVE when the d) READ SAMPLE: Samples will be consecutively requested
absorbance values do not follow an increasing or decreasing for reading. The measured absorbance is displayed as
progression. well as the concentration calculated according the selected
axis, the calculation mode and the dilution factor. In
Depending on the situation, one of the following menus will versions providing printer, the samples are consecutively
then appear: numbered and their respective absorbance values, means
and calculated concentrations are printed.
Acceptable curve e) AXIS: It allows to change the programmed selection
New curve between linear (LIN) or logaritmic (LOG) for each axis (see
3.4.1.1.2.s).
1 PRINT CURVE f) CALCULATION MODE: It allows to change the programmed
2 LIST STANDARDS calculation mode (see 3.4.1.1.2.d) among the following
3 DELETE REPLICATES (see figure 3.1):
4 READ SAMPLE
5 AXIS - POLYGONAL: Segments between consecutive points.
6 CALCULATION MODE - SPLINE: Monotonous line passing over all the points.
7 SAVE CURVE - REGRESSION LINE: Least squares line.
- REGRESSION PARABOLA: Second order polinomic
Abnormal curve regression.
New curve g) SAVE CURVE: The standard curve may be stored for
future use. The absorbance values for each standard
1 PRINT CURVE (excepting those eventually deleted) are stored, as well as
2 LIST STANDARDS the axis and the calculation mode finally selected for the
3 DELETE REPLICATES curve.
4 AXIS
5 CALCULATION MODE 3.3.5.7. Multiple-test procedures

Saved curve This type of procedures is applied in the case of tests

requiring two or more readings of the same sample , when
1 PRINT CURVE
2 LIST STANDARDS several samples are processed simultaneously whih the
3 READ SAMPLE incubation performed outside the photometer.
4 AXIS
5 CALCULATION MODE The absorbance of the samples is read sequentially and the
readings are then repeated for the same samples and in the
Select one of these options depending on the task you want same order, at fixed time intervals. In this way, two readings
to perform:
(multi-fixed time) or more (multi-kinetics) are done for a
series of samples at programmed time intervals.
19
User manual

LIN LIN

POLYGONAL SPLINE

LIN LIN

LOG LOG

REGRESSION REGRESSION
LINE PARABOLA

LOG LIN

Figure 3.1 Examples of calculation mode

The instrument then waits for the interval time to perform the
Once zeroed with the base line, the display requests the second reading of the first and the following samples,
number of samples to be processed. The number must be displaying a waiting message. The display will show again
within the range displayed, a funtion of the delay and the the sample number and a beep warns that it must be sipped.
interval times programmed. Input the number and press A second reading of all the samples is thus obtained.
ENTER.
In the case of multi-kinetics, the process will continue until
The multiple-test process begins after pressing ENTER the programmed number of intervals is completed.
again. The display shows the number of the sample to be
inserted and beeps at the precise time it must be sipped; At the end of the measurement process, the instrument will
once the programmed delay time is completed, the instrument display and print (versions providing printer) the results
requests the second sample and sequentially the rest until obtained for each sample as described for the single-test
the frist reading of all the samples is accomplished. In test procedures (see Sections 3.3.5.3. and 3.3.5.5.). The results
using a standard, it is requested and processed at the of the diverse samples and the single delta values are
beginning of the test, like in the single-test procedures. available using the VERTICAL CURSORS.

20
The same standard curve is shown in different formats 3.3.7. Ending a measurement cycle
depending on the calculation mode and the axis scale.
Any measurement procedure may be ended at any moment
3.3.6. Resolution in measurements, display, by pressing END. The process will be interrupted and the
printer and calculations program will return to the MAIN MENU.

The absorbances are measured and printed (in versions If you are measuring a standard or samples and you press
providing printer) with a resolution of 0.0001 A, although they ESCAPE, the measurement will be interrupted and the
are shown on the display rounded to the third decimal. program will return to “insert REAGENT BLANK” or to “insert
Calculations are made taking the measured absorbances BASE LINE”.
with a resolution of 0.0001 A, and then round to a maximum
of 4 digits to express concentrations.

3.3.8. Error messages

Error Message Causes of Error Solution

Base Line Error Insufficient light to make * Repeat base line

measurement reading
* Possible bubble in flow tray * Replace bulb
* Defunct lamp bulb

Reagent Blank Error Absorbency is beyond range of * Repeat reagent blank

of instrument reading
* Possible bubble in flow tray * Replace reagent
* Deteriorated reagent

Standard Value Beyond Absorbency is beyond range of * Repeat reagent blank

Absorbency Range > 2.200 instrument reading
* Possible bubble in flow tray * Replace reagent
* Deteriorated reagent

Standard Error abs <=0.000 Absorbency is beyond range of * Repeat absorbency

instrument reading for standard and
* Bubble in flow tray for standard blank if in
* Deteriorated reagent differential mode.
* In differential mode, standard * Replace reagent
value is less than standard
blank

Sample value beyond range Absorbency is beyond the * Repeat sample reading
Absorbency > 2.200 range of the instrument
* Possible bubble in flow tray * Replace reagent
* Deteriorated reagent

Abs > 2.200 Absorbency is beyond range * Repeat sample reading

of instrument
* Possible bubble in flow tray * Replace reagent
* Deteriorated reagent

21
User manual

3.4. PROGRAMMING requests different parameters according to the selected

calculation mode. Table 3.1 indicates which parameter is
PATH: PROGRAMMING required for each mode.

This option of the MAIN MENU allows you to program any On creating a Test, the parameters appear with default
of the programmable parameters of the photometer. The values.
following menu appears on the display:
You can go from one display to the previous or to the next
1 TESTS one by means of the VERTICAL CURSORS, or forward by
2 PERSONALIZE means of the ENTER key, in which case the numeric
3 FILTERS TABLE parameters that had been input are confirmed and stored.
4 UNITS Programming finishes when you reach the last parameter
5 COMMUNICATIONS and you press ENTER (or by pressing ESCAPE at any
moment), the exit menu will appear:

WARNING 1 SAVE
The pre-programmed techniques are compati- 2 QUIT (NO SAVE)
ble with Biosystems reagents. Reagents whose 3 REVIEW
measurement procedures, as described in the
instructions for use, are compatible with the
specifications of the instrument may also be EPF = END POINT WITH FACTOR.
employed. EPS = END POINT WITH STANDARD.
DKF = DELTA KINETICS WITH FACTOR.
DKS = DELTA KINETICS WITH STANDARD.
DMF = DIFFERENTIAL MODE WITH FACTOR.
DMS = DIFFERENTIAL MODE WITH STANDARD.
3.4.1. Programming tests FTF = FIXED TIME WITH FACTOR.
FTS = FIXED TIME WITH STANDARD.
PATH: PROGRAMMING/TESTS MS = MULTISTANDARD
MKS = MULTINETIC WITH STANDARD
This option lets you program the parameters of a Test, in MKF = MULTINETIC WITH FACTOR
each of the 75 locations available in the photometer. On MFS = MULTIFIXED TIME WITH STANDARD
selecting TESTS, the following menu appears: MFF = MULTIFIXED TIME WITH FACTOR
1 CREATE NEW TEST
2 REVIEW
3 DELETE
3.4.1.1.2. How to program parameters
4 COPY
5 MOVE PATHS: PROGRAMMING/TESTS/CREATE NEW TEST
PROGRAMMING/TESTS/REVIEW
CONCENTRATIONS/.../MODIFY PARAMETERS
3.4.1.1. Creating a Test
The main function of the photometer is to calculate concentra-
PATH: PROGRAMMING/TESTS/CREATE NEW TEST tions, using the analysis Tests programmed within it and
stored at a numbered location. The input or modification of
the parameters that constitute these Tests is explained in
3.4.1.1.1. General procedure this section. A diagram in appendix IV illustrates the whole
procedure and also shows the different options and the value
This option allows you to program a new location with the limits for programming.
different parameters which constitute a Test. The program
indicates which is the first free location. Press ENTER to a) LOCATION
accept it or key in a different number. If the selected location
is already programmed, an information message appears Input, using the NUMERIC KEYBOARD, the number of
on the display. the location in which it is desired to create, review or
modify a Test. The display indicates which is the first
Once the location number has been selected, a series of available location in the case of CREATE. Press ENTER
displays are presented to program each parameter. The to confirm the selected location.
way of programming each one depends on the parameter,
and will be explained in the following section. The program To CREATE A NEW TEST, a non-programmed location

22
 PARAMETER CALCULATIONMODE

# Only in versions with sipper. E E D D D D F F M M M M M

* Only in versions with printer.
P P K K M M T T S K K F F
F S F S F S F S S F S F

TESTNAME * * * * * * * * * * * * *
UNITS * * * * * * * * * * * * *
CALCULATIONMODE * * * * * * * * * * * * *
READINGMODE * * - - - - - - * - - - -
REFERENCEFILTER * * - - - - - - * - - - -
READINGFILTER * * * * * * * * * * * * *
FACTOR * - * - * - * - - - * - *
STABILIZ. TIME # * * - - * * - - * - - - -
INCUBATIONTIME - - * * - - * * - * * * *
INTERVALTIME - - * * - - * * - * * * *
INTERVALSNUMBER - - * * - - - - - * * - -
SAMPLE VOLUME # * * * * * * * * * * * * *
REACTIONSLOPE * * * * - - * * * * * * *
STANDARD - * - * - * - * - * - * -
STANDARDNUMBER - - - - - - - - * - - - -
STANDARD REPLICATES - - - - - - - - * - - - -
SAMPLEREPLICATES - - - - - - - - * - - - -
AXIS* - - - - - - - - * - - - -
STANDARD CONCENTRATIONS - - - - - - - - * - - - -
DILUCTIONFACTOR - - - - - - - - * - - - -
TEMPERATURE * * * * * * * * * * * * *
DELAY - - - - - - - - - * * * *
Table 3.1 Requested parameters according to the calculation mode

must be used or the display will indicate: Programmed

location.
Nº UNITS
Up to 75 (from 0 to 74) different locations may be
programmed for distinct Tests. 1 µg/dL
2 mg/dL
When programming the diferent parameters, an “L” and 3 g/L
the number of the location that is being programmed will 4 U/L
appear in the upper right corner of the screen. 5 µmol/L
6 mmol/L
b) TEST NAME 7 nkat/L
8 µkat/L
The name of the Test may be any title composed of one 9 g/dL
or several words, but containing a maximum of 10 10 mg/L
characters. The text must be input using the 11 IU/mL
ALPHANUMERIC input (section 2.11.1.). 12 µg/L

c) UNITS Table 3.2 Pre-programmed units

Input using the NUMERIC KEYBOARD, or select using

the HORIZONTAL CURSORS, the number of the unit that
you want to express the results. You may use either the units
programmed in factory (table 3.2) or the units you may have
programmed (section 3.4.4.).

23
User manual

d) CALCULATION MODE e) READING MODE

Select using the HORIZONTAL CURSORS the calculation Only in the END POINT and MULTISTANDARD modes.
mode to be employed among the following available: Select by means of the HORIZONTAL CURSORS one of
the options.
END POINT WITH FACTOR
END POINT WITH STANDARD MONOCHROMATIC: A single measurement is made of
DELTA KINETICS WITH FACTOR each sample at the selected wavelength.
DELTA KINETICS WITH STANDARD
DIFFERENTIAL MODE WITH FACTOR BICHROMATIC: Two measurements of each sample are
DIFFERENTIAL MODE WITH STANDARD made (figure 3.2), one at the main wavelength (usually at
FIXED TIME WITH FACTOR the maximum absorbance of the liquid being measured),
FIXED TIME WITH STANDARD and another at the secondary or reference wavelength
MULTISTANDARD (generally far from the maximum absorbance of the liquid
MULTIKINETIC WITH FACTOR being measured). The absorbance value used for
MULTIKINETIC WITH STANDARD calculations is in this case the difference between both
MULTIFIXED TIME WITH FACTOR absorbances (A = Amain - Areference).
MULTIFIXED TIME WITH STANDARD

If MULTISTANDARD is selected, you may use one of the

following functions (see 3.3.5.6.f): POSITION FILTER

POLYGONAL 1 340
SPLINE 2 405
REGRESSION LINE 3 505
REGRESSION PARABOLA 4 546
5 600
Select by means of the HORIZONTAL CURSORS one of 6 635
the options and press ENTER. 7 670
8 ---

Figure 3.3 Filters table

Abs A= Am - Ar
Am

Ar
l
main l reference l

Figure 3.2 Bichromatic reading

24
f) REFERENCE FILTER l) INTERVALS NUMBER

Only in BICHROMATIC reading mode. Input by means of Only in the mode KINETICS. Number of intervals (deltas)
the NUMERIC KEYBOARD, or select using the HORI- desired. Equivalent to the total number of measurements
ZONTAL CURSORS, the secondary or reference minus 1. Input the number using the NUMERIC
wavelength in nanometers. If the filter with the selected KEYBOARD.
wavelength has not been programmed in the FILTERS
TABLE, an error message will appear in the display. Table m) SAMPLE VOLUME (Only in version with sipper)
3.3 shows the filters the photometer is usually equiped
with, and their respective positions. Volume (in mL) of sample to be sipped for the
measurements. Minimum programmable value is 100
g) READING FILTER mL. Take into account that the less the volume sipped,
the more will be the carryover contamination between
Input by means of the NUMERIC KEYBOARD, or select consecutive samples. Table 3.4 shows an evaluation of
using the HORIZONTAL CURSORS, the wavelength in carryover for different sample volumes. Take into account
nanometers at which it is desired to make the that these values may considerably vary depending on
measurements. In BICHROMATIC mode, it refers to the the nature of the liquid being measured. Input the volume
main wavelength (figure 3.2). If the filter with the selected using the NUMERIC KEYBOARD.
wavelength has not been programmed in the FILTERS
TABLE, an error message will appear in the display. Table
3.3 shows the filters the photometer is usually equipped
with, and their respective positions. SAMPLE VOLUME (%)

h) FACTOR 150mL 2.25 - 2.70

250mL 0.75 - 1.50
Input by means of the NUMERIC KEYBOARD the value 400mL 0.05 - 0.25
of the factor to use in the calculations for transforming
absorbances into concentrations expressed in the selected Table 3.4 Carryover contamination
units (section 3.4.1.1.2.c).
n) REACTION SLOPE
i) STABILIZATION TIME (Only in version with sipper)
Select with the HORIZONTAL CURSORS:
Time elapsed between the end of the sipping cycle and the
measurement in the modes END POINT, DIFFERENTIAL INCREASING: When the absorbance increases with
and MULTISTANDARD. It is usually one second, but concentration (end point, differential, multistandard) or
other values can be programmed, allowing the increases with time (delta kinetics, fixed time).
measurement to be delayed until the liquid homogeneizes
perfectly in the cuvette, specially when working with DECREASING: When the absorbance decreases with
highly viscous liquids. Input the time using the NUMERIC concentration (end point, differential, multistandard) or
KEYBOARD. decreases with time (delta kinetics, fixed time).

o) STANDARD
j) INCUBATION TIME
Appears for the calculation modes using a single stan-
Time elapsed from pressing ENTER (or PUMP in version dard. Input, by means of the NUMERIC KEYBOARD, the
with sipper) and the first measurement in the modes concentration value of the standard, in the same units
KINETICS and FIXED TIME. Input the time using the programmed to express the results.
NUMERIC KEYBOARD.
p) STANDARD NUMBER
k) INTERVAL TIME
Appears for the calculation mode MULTISTANDARD.
Time elapsed between two consecutive measurements of Input the total number of standards to be used by means
absorbance in the mode KINETICS, and time elapsed of the NUMERIC KEYBOARD.
between the first and the second measurement in the
mode FIXED TIME. Input the time using the NUMERIC q) STANDARD REPLICATES
KEYBOARD.
Appears only in the mode MULTISTANDARD. Input the
number of replicates to be required for each standard and
for the reagent blank using the NUMERIC KEYBOARD.

25
User manual

r) SAMPLE REPLICATES parameters that may have been input, select the option
QUIT (NO SAVE).
Appears only in the mode MULTISTANDARD. Input the
number of replicates to be required for each sample using It is also possible to review the programmed parameters by
the NUMERIC KEYBOARD. selecting REVIEW. You can modify any value during this
process and validate it at the end of the reviewing by
s) AXIS selecting SAVE.

Appears only in the mode MULTISTANDARD. Select 3.4.1.2. Reviewing a Test

using the HORIZONTAL CURSORS the desired scales for
the absorbance versus concentration plot and for the PATH: PROGRAMMING/TESTS/REVIEW
calculations, among the following:
ABS........... LIN LIN LOG LOG This option will allow you to review and eventually modify the
CON.......... LIN LOG LIN LOG values programmed for the parameters in a location which
has been already programmed and stored in memory.
t) STANDARD CONCENTRATIONS
The display requires the location number you want to review.
Appears only in the mode MULTISTANDARD. Input, Once the number has been input, you go into the same
using the NUMERIC KEYBOARD, the concentration routine as in CREATE NEW TEST appearing the different
value of each standard, in the same units programmed to parameters with the programmed values in a sequential
express the results. By pressing ENTER, the display will mode. You may then modify any of them.
request the concentration of the next standard.
After the last parameter or by pressing ESC at any time, the
u) DILUTION FACTOR exit menu appears on the display allowing you to save, quit
or review the location again.
Appears only in the mode MULTISTANDARD. It is used
when all the samples are diluted but the standards are not
diluted. Input by means of the NUMERIC KEYBOARD, 3.4.1.3. Delete a test
the dilution factor value.
PATH: PROGRAMMING/TESTS/DELETE
v) TEMPERATURE
This option allows to delete the contents of a programmed
Temperature at which it is desired to thermostat the location. Once selected, the display requires the location to
cuvette. Programmable between 23 and 40°C, with a be deleted. Input by means of the numeric keyboard the
resolution of one degree. If it is not desired to thermostatize, number of the location and the display will show (example
input “0”. Use the NUMERIC input. of deleting location 23):
w) DELAY DELETE LOCATION 23
are you sure?
Minimum time elapsed between consecutive samples in
the multiple procedures. <YES> <NO>

If you press ENTER/YES, the programmed values for the

3.4.1.1.3. Exit menu parameters of the location will be deleted and the location
will remain free to be retrieved when creating a test. If you
Immediately after programming the last parameter or by press ESC./NO, you will exit to the previous menu, without
pressing ESC at any moment during the programmation of altering the contents of the location.
parameters, the following menu appears on the display:
When the selected location is not programmed, a caution
1 SAVE message is issued.
2 QUIT (NO SAVE)
3 REVIEW
3.4.1.4. Copy a test
If you select SAVE, the programmed parameters will remain PATH: PROGRAMMING/TESTS/COPY
permanently stored in the memory of the apparatus, and
the program will go back to the previous menu. This option eneables you to copy the contents of a location
into another, without deleting the contents of the first. It is
If you want to abandon the programming without saving the

26
specially useful to program a test that is very similar to an * Version with printer and sipper
existing one. The test can be copied in a new location and 1 LANGUAGE
afterwards it can be reviewed to modify a few parameters. 2 SCREEN CONTRAST
To make the copy, the following display appears: 3 LABORATORY NAME
4 WASH MODE
5 HEADING LIST
COPY TEST 6 PRINTER CONTROL
Source location = _ 7 PATIENT CODE
Target location =
* Version with printer and without sipper

1 LANGUAGE
Enter into “Source location” the number of the location you 2 SCREEN CONTRAST
want to copy, and into “Target location” the new location 3 LABORATORY NAME
where you want it to be copied. On pressing ENTER a 4 HEADING LIST
second time, the program copies the contents of the source 5 PRINTER CONTROL
location into the target location. 6 PATIENT CODE

If the source location is not programmed, as well as if the * Version without printer and without sipper
target location is already programmed, caution messages
are issued and the program does not confirm the location 1 LANGUAGE
numbers until they are correct. 2 SCREEN CONTRAST
3 PATIENT CODE
3.4.1.5. Move a test

PATH: PROGRAMMING/TESTS/MOVE ATTENTION

The selected options in programming
This option enables you to change the location of a test, personalize are stored by pressing ENTER. To
without having to re-program it. The following display appears: abandon, press ESC.

MOVE TEST 3.4.2.1. Language

Source location = _
Target location = PATH: PROGRAMMING/PERSONALIZE/LANGUAGE

By means of this option, the user may select the language,

among those available, in which the photometer is to
Enter into “Source location” the number of the location you communicate through the display and print the texts in
want to move, and into “Target location” the new location versions providing printer. To select the language use the
where you want it to remain. On pressing ENTER a second HORIZONTAL CURSORS and press ENTER with your
time, the program copies the contents of the source selection on the display.
location into the target location, and deletes the first one.

If the source location is not programmed, as well as if the 3.4.2.2. Laboratory name (only in versions
target location is already programmed, caution messages with printer)
are issued and the program does not confirm the location
numbers until they are correct. PATH: PROGRAMMING/PERSONALIZE/LABORATORY
NAME
3.4.2. Personalization of the photometer
This option enables you to program a title of maximum 10
PATH: PROGRAMMING/PERSONALIZE alphanumeric characters, using the ALPHANUMERIC
INPUT procedure. Once the title has been input, press
This option enables the user to adapt the photometer to his ENTER.
personal preferences in some aspects. A diagram in
appendix VI illustrates the programming process of these The programmed text will be printed each time the photometer
personalized parameters. With this option, one of the is switched on.
following menus appears:

27
User manual

3.4.2.3. Screen contrast This option enables you to adjust the intensity of black
print on the thermal paper of the printer in such a way that
PATH: PROGRAMMING/PERSONALIZE/SCREEN slight variations in the paper quality can be compensated
CONTRAST for. It also allows you to de-activate the printer.

This option allows adjusting the screen contrast. The Once selected this option, the following appears on the
contrast change from 1 to 10, from less to more intensity. display:
By means of VERTICAL CURSORS it is possible to change
the value, this is shown on the display. PRINTER CONTROL
CONTRAST.........= _
3.4.2.4. Selection of the wash mode (only in THERMAL PRINTER
1 CHECK
versions with sipper)

PATH: PROGRAMMING/PERSONALIZE/WASH MODE The print intensity (contrast) is defined by a number from 1
to 10 which may be varied by means of the HORIZONTAL
The WASH key is used to circulate a wash solution or water CURSORS. By pressing key 1, a line of asterisks is printed
through the sipping circuit for the purpose of cleaning it at allowing you to check the selected intensity.
the end of a series of measurements or between samples.
By means of this option, it is possible to select using the By using the VERTICAL CURSORS you can deactivate
HORIZONTAL CURSORS, two washing modes: the printer (or activate it, if previously de-activated).
CONTINUOUS: The pump continuosly functions while the Quit with ENTER.
WASH key is held down.

FIXED VOLUME: On pressing WASH, a pre-set volume will

3.4.2.7. Patient code
be sipped. Input the desired wash volume (mL) by means of
the numeric keyboard and confirm it by pressing ENTER. PATH: PROGRAMMING/PERSONALIZE/CODE PATIENT

Once your option is selected on the display, quit by This option allows you to activate or deactivate the patient
pressing ENTER. code. When the patient code is active, you have to enter a
code before entering each sample. This code identifies the
sample results in the printer and in the data information
3.4.2.5. List headings (only in versions with transmitted to the PC through the serial port.
printer)

PATH: PROGRAMMING/PERSONALIZE/LIST HEADINGS 3.4.3. Programming the filters table

When absorbances or concentrations are going to be PATH: PROGRAMMING/FILTERS TABLE
measured, some information about it is automatically printed,
consisting in a fixed part and another optional. The fixed part
of the heading has been described in sections 3.2 and 3.3. The filters wheel has 9 positions, numbered from 1 to 9.
The optional part can be selected in this option of the menu. The filters table is pre-programmed in the factory for the
By selecting LIST HEADINGS, the following options may be filters which are provided with the photometer. However,
selected by using the HORIZONTAL CURSORS: these may be changed from one position to another, or
new filters may be added.
NO PARAMETERS: Only the fixed part is printed.
On selecting FILTERS TABLE, this display appears:
PRINT PARAMETERS: All the Test parameters with their
programmed values are printed.
1 > 340 5 = 546 8 = 670
Once an option has been selected on the display, quit by 2 = 405 6 = 600 9 = - - -
pressing ENTER. 4 = 505 7 = 635

3.4.2.6. Adjusting the printer (only in versions

with printer) (or other values that may have been programmed in factory
according to the filters provided).
PATH: PROGRAMMING/PERSONALIZE/PRINTER CON-
TROL The symbol “>” indicates the position that can be modified,
and its first digit has the input cursor. Key in a new number
28
and press ENTER to input the new value for that position. 1 CONFIG RS-232-C
The sign “>” goes on to the next filter number. With the 2 DEFAULT PARAMETER
VERTICAL CURSORS you can run through the table without
modifying the values. On arriving at a free position (- - -), a 3.4.5.1. Configuration of RS-232-C
0 appears. The range of filters is from 340 nm to 700 nm. If
a number outside this range is keyed in, “Err” appears in PATH: PROGRAMMING/COMMUNICATIONS/CONFIG
the lower right corner. Input again a correct number. Key in RS-232-C
0 in new free positions.
This allows you to program the three parameters that are
Quit by pressing ESC. output on the following display (default values):

3.4.4. Programming units

BAUDS ....... >9600
PATH: PROGRAMMING/UNITS TIMEOUT...........=0
TERMINAL........=0
This option allows you to program the table of units. This
table consists of a set of up to 50 positions in which titles
of up to 8 characters can be programmed and stored. These Using the VERTICAL CURSORS the parameter to be
titles (units) will be displayed or printed together with the modified is selected, while the HORIZONTAL CURSORS
concentration values when operating in CONCENTRATIONS. are used to change values. The following values can be
The most common units come pre-programmed from the selected:
factory (table 3.2), but you can change them, or add new
ones, according to your needs. On selecting UNITS, this - Transmission speed (bauds): 110, 150, 300, 600, 1200,
display appears: 2400, 4800, 9600, 19200.
- Timeout: 0-250
- Terminal: 0-32

UNITS Once your option is selected on the display, quit with

Number ....... = _ ENTER to save values or press ESC to cancel any
modification.

3.4.5.2. Default parameters

Input the number of the position you want to program (or PATH: PROGRAMMING/COMMUNICATIONS/DEFAULT
change) a title for a unit, and a row of 8 hyphens (if the PARAMETER.
position has no title), or the previous title, will appear on the
display. Examples:
Select a fixed set of communication parameters:

UNITS BAUDS ........... 9600

Number ..... = 25 TIMEOUT .............. 0
-------- TERMINAL ............ 0

UNITS 3.5. LISTING

Number ..... = 2
mg/dL- - - PATH: LIST
Using the ALPHANUMERIC INPUT procedure, the title of With this option you can obtain different listings. On
the desired unit can be programmed or modified. Press ESC selecting the option, the following menu appears only in
to quit. versions providing printer:

3.4.5. Programming communications

1 DISPLAY
PATH: PROGRAMMING/COMMUNICATIONS 2 PRINTER

On selecting this option, the menu appears: This allows you to list the information in the display

29
User manual

selecting DISPLAY, or print it selecting PRINTER. 3.5.2.1. Listing tests

3.5.1. Listing the display PATH: LIST/PRINTER/TESTS

PATH: LIST/DISPLAY (In versions with printer) If you select this option, the following menu appears:
LIST (In versions without printer)
1 TEST NAMES
2 TEST CONTENTS
On selecting this option, the following menu appears:
If there is no programmed location, an information message
1 TESTS is displayed instead of the menu.
2 UNITS
3.5.2.1.1. Listing of the tests names

3.5.1.1. Listing tests PATH: LIST/PRINTER/TESTS/TEST NAMES

PATH: LIST/DISPLAY/TESTS (In versions with printer) The numbers of the programmed locations will be listed,
LIST/TESTS (In versions without printer) along with the name of the Tests that each one contains.

3.5.2.1.2. Listings of tests parameters

If you select this option, the number and title of the first
programmed location appears on the display. You can PATH: LIST/PRINTER/TESTS/TEST CONTENTS
review these data of all the programmed locations by using
the VERTICAL CURSORS. Pressing the RIGHT CURSOR With this option, you can list the contents of the programmed
at any moment returns you to the first programmed location, locations. The program requires on the display the numbers
and pressing the LEFT CURSOR brings you to the last of the first and last location to be listed.
programmed location. Press ESC to quit.

LIST TEST
3.5.1.2. Listing units From ............ =_
To................. =
PATH: LIST/DISPLAY/UNITS (In versions with printer)
LIST/UNITS (In versions without printer)
Key in the numbers of the first and last location you want
If you select this option, the number and title of the first to list and press ENTER. The complete data contained in
programmed unit appears on the display. You can review each location will be immediately printed.
these data of all the programmed units by using the
VERTICAL CURSORS. Pressing the RIGHT CURSOR at If no test is programmed in the selected range, a caution
any moment returns you to the first programmed unit, and message appears.
pressing the LEFT CURSOR brings you to the last
programmed unit. Press ESC to quit. 3.5.2.2. Listing the personalization
parameters
3.5.2. Listing in the printer (only in versions PATH: LIST/PRINTER/PERSONALIZE
with printer)
On selecting this option, a list is printed showing the titles
PATH: LIST/PRINTER or options that have been programmed in personalization
(section 3.4.2).
On selecting this option, the following menu appears:
3.5.2.3. Listing the filters table
1 TESTS
2 PERSONALIZE PATH: LIST/PRINTER/FILTERS TABLE
3 FILTERS TABLE
4 UNITS This option enables you to list a table with the number and
5 COMMUNICATIONS the wavelength of each programmed filter.
6 PUMP PARAMETERS (only in versions with sipper) (Section 3.4.3).

30
3.5.2.4. Listing units a) Fill the cuvette with distilled water and insert it into the
cuvette holder. Use the smallest cuvette you normally
PATH: LIST/PRINTER/UNITS work with. In version providing sipper fill the flow cuvette
with distilled water by pressing WASH.
This option lists a table with the number and title of each
programmed unit. b) Select the menu OTHER OPTIONS and press CHECK
(Section 3.4.4). LAMP.

c) If the lamp is properly centered the display will show:

3.5.2.5. Listing the communication
parameters CHECK LAMP

PATH: LIST/PRINTER/COMMUNICATIONS
10.254 nA PASSED
This option enables you to list the parameters of the channel
that are programmed at the time (section 3.4.5.). d) In case the lamp has not been properly installed, the
display will show:
3.5.2.6. Listing the pump parameters
CHECK LAMP
PATH: LIST/PRINTER/PUMP PARAMETERS

On selecting this option, a list is printed showing the values 0.254 nA NO PASSED
that have been programmed in pump parameters (section
3.7.3).
The value shows the photodiode current with the 340nm
3.6. OTHER OPTIONS filter, it is expressed with nanoamperes (nA).

PATH: OTHER OPTIONS In this case, repeat the procedure for changing the lamp
described in section 5.3. but turning the lamp-holder 180
This option of the main menu includes some less frecuently degrees, and repeat the checking. If the lamp does not pass
used jobs. the test again, it is defective and must be replaced with
another one.
The following menu appears on the display:
3.6.3. Adjust of the peristaltic pump (only in
versions with sipper)
1 SERVICE
2 CHECK LAMP PATH: OTHER OPTIONS/ADJUST PUMP
3 ADJUST PUMP
4 LOAD A NEW PROGRAM Through usage and with the passage of time, the silicone
5 DELETE CONCENTRATIONS tubing of the peristaltic pump suffers slight deformation
which leads to the SAMPLE VOLUME not being exactly the
3.6.1. Service same as that which had been programmed. Because of this,
it is advisable to perform a adjust of the peristaltic pump
PATH: OTHER OPTIONS/SERVICE periodically and also after changing the tubing of the pump.

This option permits the instrument to be adjusted in those Besides adjusting the SAMPLE VOLUME, the
areas reserved for the technical assistance service. When POSITIONING of the sample in the cuvette, and the DELAY
this option is selected, the apparatus requests an ACCESS TIME, can also be adjusted.
PASSWORD only known by authorized staff.
Upon selecting this option, the following sub-menu appears
3.6.2. Check lamp on the display:

PATH: OTHER OPTIONS/CHECK LAMP 1 CHECK

2 MANUAL
This option allows you to check that the lamp has been 3 AUTOMATIC
properly centered when it has been changed (section 5.3).
Proceed as follows:

31
User manual

3.6.3.1. Checking adjust of the sample Key in the estimated number and press ENTER. The new
volume value will be stored and used in the next sipping cycles to
calculate the sample volume. You may check it by going to
PATH: OTHER OPTIONS/ADJUST PUMP/CHECK the previous menu (press ESC) and selecting CHECK.

This option allows you to enter a SAMPLE VOLUME 3.6.3.2.2. Sample positioning
between 100 and 5000 mL, and to perform pump cycles in
order to check the programmed parameters. On entering PATH: OTHER OPTIONS/ADJUST PUMP/MANUAL/
into the option, key in the value of the SAMPLE VOLUME, POSITIONING
and press ENTER. Then the instrument indicates:
The value for this parameter is pre-programmed in factory,
and is the number of steps the pump must run, after sipping
CHECK PUMP and the pump delay time, to position the sample in the
cuvette. It is said that the sample is positioned when
practically all the sipped sample has passed through the
Insert TUBE cuvette, cleaning the previous sample, and only about 5 mm
of the tubing immediatelly before the cuvette is filled with
sample. If it is seen that a longer part of the sipped sample
From this moment on, you can perform as many sipping remains without entering into the cuvette, this number must
cycles as you wish by pressing PUMP. Take into account be increased, in the opposite case it must be decreased. In
that here no photometric measurement is performed. the manual procedure the number must be a rough estimate.

During the cycles check that the sipped volume is the The following display appears:
required and that the sample is correctly transported to the
cuvette.
POSITIONING
3.6.3.2. Manual adjust Actual value = 178
New value ...=_
PATH: OTHER OPTIONS/ADJUST PUMP/MANUAL

With this option, the following menu appears:

Key in the estimated number and press ENTER. The new
1 SAMPLE VOLUME value will be stored and used in the next sipping cycles to
2 POSITIONING position the sample. You may check it by going to the
3 PUMP DELAY previous menu (press ESC) and selecting CHECK.

3.6.3.2.1. Sample volume

3.6.3.2.3. Pump delay time
PATH: OTHER OPTIONS/ADJUST PUMP/MANUAL/
SAMPLE VOLUME PATH: OTHER OPTIONS/ADJUST PUMP/MANUAL/
PUMP DELAY
This parameter is a number which is pre-programmed in the
factory and means the number of steps the pump must run By means of this option you can adjust the delay time to
in order to sip 1000mL of sample. If a previous check showed your preferences.
that the pump sipped less volume than what was
programmed, this number should be increased. If it was The following display appears:
found that the volume sipped was more than programmed,
the sipping number must be decreased. In the manual
procedure, it must be done as a rough estimate, measuring PUMP DELAY
the volume sipped by weight or any other reliable method. Actual value = 2
New value ...=_
The following display appears:

Key in the number of seconds you wish and press ENTER.

SAMPLE VOLUME The new value will be stored and used in the next sipping
Actual value = 906 cycles to give you time to pull the tube or vessel with sample
New value ...=_ out before positioning.

32
3.6.3.3. Automatic adjust appears the received bytes and the saved bytes.
The following screen appears:
PATH: OTHER OPTIONS/ADJUST PUMP/AUTOMATIC
CHANGE PROGRAM
With this option, the following menu appears: 1 CONFIGURATION
2 EXIT
1 SAMPLE VOLUME RECEIVED=2039 SAVING
2 POSITIONING SAVED=156789

3.6.3.3.1. Sample volume At the end of the process the following message appears:

PATH: OTHER OPTIONS/ADJUST PUMP/AUTOMATIC/ End Communications

SAMPLE VOLUME
Once this message appears, you can press the key number
Pipette 5 mL of distilled water into a test tube and sip it by 2, and quit from this option. The photometer restarts with a
pressing ENTER. About 4 mL will be sipped at normal speed new version program.
and then the pump will reduce speed. Press again ENTER All this operation could last several minutes.
at the moment when the complete volume has JUST been
sipped from the tube. Take care that the end of the sipper
tubing be immersed into the water during the whole process. WARNING
The sipping number will have been automatically adjusted.
If a problem occurs while you are downloading
the program, such as a power supply cut, and
3.6.3.3.2. Sample positioning the photometer does not switch on properly, you
should turn off the photometer and switch it on
PATH: OTHER OPTIONS/ADJUST PUMP/AUTOMATIC/ again while holding down the PUMP button.
POSITIONING This will cause the photometer to enter the
LOAD A NEW PROGRAM menu directly where
Fill a test tube with distilled water and sip it by pressing you can repeat the process.
ENTER. When the display indicates it, remove the tube and
press ENTER again. A few seconds later, the display will
indicate that the process has terminated. The positioning of
the sample has been automatically adjusted.
3.6.5. Delete the concentration values

Each time you perform a concentration measurement, the

3.6.4. Load a new program value is saved in the photometer memory. This memory has
the capacity of 100 concentration values. The PC application
This allows you to change the photometer program version. supplied with the photometer allows you to access these
To do this operation, you need a special PC application concentration values through the communication port. Once
supplied with the photometer that manages all the readed these values will be deleted from memory.
communications with the apparatus.
If you perform 100 measurements without reading them from
When performing this process, all text appears in English, the PC, the next measure you perform will delete the first
without any option to change the language. measure and then successively.
The following screen appears on the display: If you want to delete all the memorised values without
sending to the PC you can do it with this option.
CHANGE PROGRAM
1 CONFIGURATION PATH: OTHER OPTIONS/DELETE CONCENTRATIONS
2 EXIT
The photometer ask:
With the first option you can configure the communication
channel like in section 3.4.5.1..
ARE YOU SURE?
After that the apparatus keeps waiting to receive the new
program. <YES> <NO>
From the PC application you select the option: change the
program version, then press the start button and begin the Press ENTER/YES and all the memorised values will be
transmission data. In the photometer side, on the screen erased. Press ESC/NO, exit without deleting the values.

33
User manual

APPENDIX I: MENUS TREE. VERSION WITH PRINTER AND

SIPPER.

LEVEL I LEVEL II LEVEL III LEVEL IV

1 ABSORBANCES 1 MONOCHROMATIC
2 BICHROMATIC

2 CONCENTRATIONS 1 MODIFY PARAMETERS

2 BASE LINE
3 REAGENT BLANK (1)
4 LIST

1 CREATE NEW TEST

3 PROGRAMMING 1 TESTS 2 REVIEW
3 DELETE
4 COPY
5 MOVE

1 LANGUAGE
2 PERSONALIZE 2 SCREEN CONTRAST
3 FILTERS TABLE 3 LABORATORY NAME
4 UNITS 4 WASH MODE
5 LIST HEADINGS
6 PRINTER CONTROL
7 PATIENT CODE
5 COMMUNICATIONS
6 USER OPTIONS 1 CONFIG RS-232-C
2 DEFAULT
PARAMETER

4 LIST 1 DISPLAY 1 TESTS

2 UNITS
2 PRINTER 1 TESTS 1 TESTS NAMES
2 PERSONALIZE 2 TESTS CONTENTS
3 FILTERS TABLE
4 UNITS
5 COMMUNICATIONS
6 PUMP PARAMETERS
7 USER OPTIONS
5 OTHER OPTIONS 1 SERVICE
2 CHECK LAMP

3 ADJUST PUMP 1 CHECK

4 COMMUNICATIONS 2 MANUAL 1 SAMPLE VOLUME
2 POSITIONING
3 PUMP DELAY

3 AUTOMATIC 1 SAMPLE VOLUME

2 POSITIONING

(1) This option may not appear, depending on the CALCULATION MODE and TYPE OF REACTION

34
APPENDIX I: MENUS TREE. VERSION WITH PRINTER AND
WITHOUT SIPPER.

LEVEL I LEVEL II LEVEL III LEVEL IV

1 ABSORBANCES 1 MONOCHROMATIC
2 BICHROMATIC

2 CONCENTRATIONS 1 MODIFY PARAMETERS

2 BASE LINE
3 REAGENT BLANK (1)
4 LIST

1 CREATE NEW TEST

3 PROGRAMMING 1 TESTS
2 REVIEW
3 DELETE
4 COPY
5 MOVE

1 LANGUAGE
2 PERSONALIZE 2 SCREEN CONTRAST
3 FILTERS TABLE
3 LABORATORY NAME
4 UNITS
4 LIST HEADINGS
5 PRINTER CONTROL
6 PATIENT CODE
5 COMMUNICATIONS
1 CONFIG RS-232-C
6 USER OPTIONS 2 DEFAULT
PARAMETER

4 LIST 1 DISPLAY 1 TESTS

2 UNITS
2 PRINTER 1 TESTS NAMES
1 TESTS
2 PERSONALIZE 2 TESTS CONTENTS
3 FILTERS TABLE
4 UNITS
5 COMMUNICATIONS
6 USER OPTIONS

5 OTHER OPTIONS 1 SERVICE

2 CHECK LAMP
3 LOAD A NEW
PROGRAM
4 DELETE THE
CONCENTRATION
VALUES

(1) This option may not appear, depending on the CALCULATION MODE and TYPE OF REACTION

35
User manual

APPENDIX I: MENUS TREE. VERSION WITHOUT PRINTER

AND WITHOUT SIPPER.

LEVEL I LEVEL II LEVEL III

1 ABSORBANCES 1 MONOCHROMATIC
2 BICHROMATIC

2 CONCENTRATIONS 1 MODIFY PARAMETERS

2 BASE LINE
3 REAGENT BLANK (1)

1 CREATE NEW TEST

3 PROGRAMMING 1 TESTS 2 REVIEW
3 DELETE
4 COPY
5 MOVE

1 LANGUAGE
2 PERSONALIZE 2 SCREEN CONTRAST
3 FILTERS TABLE 3 PATIENT CODE
4 UNITS

5 COMMUNICATIONS 1 CONFIG RS-232-C

6 USER OPTIONS 2 DEFAULT
PARAMETER

4 LIST 1 TESTS
2 UNITS

5 OTHER OPTIONS 1 SERVICE

2 CHECK LAMP
3 LOAD NEW PROGRAM
4 DELETE THE
CONCENTRATIONS
VALUES

(1) This option may not appear, depending on the CALCULATION MODE and TYPE OF REACTION

36