SAFETY DATA SHEET Version 6.

4
Revision Date 16.07.2021
according to Regulation (EC) No. 1907/2006 Print Date 10.09.2021
GENERIC EU MSDS - NO COUNTRY SPECIFIC DATA - NO OEL DATA

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifiers
Product name : Acrylamide/Bis-acrylamide, 30% solution
Product Number : A3574
Brand : Sigma
REACH No. : This product is a mixture. REACH Registration Number see
section 3.

1.2 Relevant identified uses of the substance or mixture and uses advised against
Identified uses : Laboratory chemicals, Manufacture of substances
1.3 Details of the supplier of the safety data sheet
Company : Sigma-Aldrich Chemie GmbH
Eschenstrasse 5
D-82024 TAUFKIRCHEN
Telephone : +49 (0)89 6513-1130
Fax : +49 (0)89 6513-1161
E-mail address : technischerservice@merckgroup.com
1.4 Emergency telephone
Emergency Phone # : 0800 181 7059 (CHEMTREC Deutschland)
+49 (0)696 43508409 (CHEMTREC
weltweit)

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008
Acute toxicity, Oral (Category 4), H302
Acute toxicity, Inhalation (Category 4), H332
Skin irritation (Category 2), H315
Eye irritation (Category 2), H319
Skin sensitization (Category 1), H317
Germ cell mutagenicity (Category 1B), H340
Carcinogenicity (Category 1B), H350
Reproductive toxicity (Category 2), H361f
Specific target organ toxicity - repeated exposure, Oral (Category 1), Peripheral nervous
system, H372
For the full text of the H-Statements mentioned in this Section, see Section 16.

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2.2 Label elements
Labelling according Regulation (EC) No 1272/2008
Pictogram

Signal word Danger

Hazard statement(s)
H302 + H332 Harmful if swallowed or if inhaled.
H315 Causes skin irritation.
H317 May cause an allergic skin reaction.
H319 Causes serious eye irritation.
H340 May cause genetic defects.
H350 May cause cancer.
H361f Suspected of damaging fertility.
H372 Causes damage to organs (Peripheral nervous system) through
prolonged or repeated exposure if swallowed.
Precautionary statement(s)
P280 Wear protective gloves/ protective clothing/ eye protection/ face
protection/ hearing protection.
P301 + P312 IF SWALLOWED: Call a POISON CENTER/ doctor if you feel
unwell.
P302 + P352 IF ON SKIN: Wash with plenty of water.
P304 + P340 + P312 IF INHALED: Remove person to fresh air and keep comfortable
for breathing. Call a POISON CENTER/ doctor if you feel unwell.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue
rinsing.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
Supplemental Hazard none
Statements
Restricted to professional users.
Reduced Labeling (<= 125 ml)
Pictogram

Signal word Danger

Hazard statement(s)
H317 May cause an allergic skin reaction.
H340 May cause genetic defects.
H350 May cause cancer.
H372 Causes damage to organs through prolonged or repeated
exposure if swallowed.
H361f Suspected of damaging fertility.
Precautionary statement(s)
P280 Wear protective gloves/ protective clothing/ eye protection/ face
protection/ hearing protection.
P302 + P352 IF ON SKIN: Wash with plenty of water.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
Supplemental Hazard none
Statements

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2.3 Other hazards
This substance/mixture contains no components considered to be either persistent,
bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at
levels of 0.1% or higher.

SECTION 3: Composition/information on ingredients

3.2 Mixtures
Component Classification Concentration
acrylamide Included in the Candidate List of Substances of Very High Concern (SVHC)
according to Regulation (EC) No. 1907/2006 (REACH)
CAS-No. 79-06-1 Acute Tox. 3; Acute Tox. >= 30 - < 50
EC-No. 201-173-7 4; Skin Irrit. 2; Eye Irrit. %
Index-No. 616-003-00-0 2; Skin Sens. 1; Muta. 1B;
Registration 01-2119463260-48- Carc. 1B; Repr. 2; STOT
number XXXX RE 1; H301, H332, H312,
H315, H319, H317, H340,
H350, H361f, H372
N,N'-methylenediacrylamide
CAS-No. 110-26-9 Acute Tox. 3; Muta. 1B; >= 1 - < 10
EC-No. 203-750-9 H301, H340 %

*
*A registration number is not available for this substance as the substance or its use are
exempted from registration according to Article 2 REACH Regulation (EC) No 1907/2006,
the annual tonnage does not require a registration or the registration is envisaged for a
later registration deadline.
For the full text of the H-Statements mentioned in this Section, see Section 16.

SECTION 4: First aid measures

4.1 Description of first-aid measures
General advice
Show this material safety data sheet to the doctor in attendance.
If inhaled
After inhalation: fresh air. Immediately call in physician. If breathing stops: immediately
apply artificial respiration, if necessary also oxygen.
In case of skin contact
In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with
water/ shower. Consult a physician.
In case of eye contact
After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact
lenses.
If swallowed
After swallowing: immediately make victim drink water (two glasses at most). Consult a
physician.

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4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section
2.2) and/or in section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available

SECTION 5: Firefighting measures

5.1 Extinguishing media
Suitable extinguishing media
Water Foam Carbon dioxide (CO2) Dry powder
Unsuitable extinguishing media
For this substance/mixture no limitations of extinguishing agents are given.
5.2 Special hazards arising from the substance or mixture
Carbon oxides
Nitrogen oxides (NOx)
Ammonia
Carbon oxides
Nitrogen oxides (NOx)
Mixture with combustible ingredients.
Development of hazardous combustion gases or vapours possible in the event of fire.
5.3 Advice for firefighters
Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by
keeping a safe distance or by wearing suitable protective clothing.
5.4 Further information
Suppress (knock down) gases/vapors/mists with a water spray jet. Prevent fire
extinguishing water from contaminating surface water or the ground water system.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures
Advice for non-emergency personnel: Do not breathe vapors, aerosols. Avoid substance
contact. Ensure adequate ventilation. Evacuate the danger area, observe emergency
procedures, consult an expert.
For personal protection see section 8.
6.2 Environmental precautions
Do not let product enter drains.
6.3 Methods and materials for containment and cleaning up
Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions
(see sections 7 and 10). Take up carefully with liquid-absorbent material (e.g.
Chemizorb®). Dispose of properly. Clean up affected area.
6.4 Reference to other sections
For disposal see section 13.

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SECTION 7: Handling and storage
7.1 Precautions for safe handling
Advice on safe handling
Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols.
Hygiene measures
Immediately change contaminated clothing. Apply preventive skin protection. Wash hands
and face after working with substance.
For precautions see section 2.2.
7.2 Conditions for safe storage, including any incompatibilities
Storage conditions
Tightly closed. Keep in a well-ventilated place. Keep locked up or in an area accessible only
to qualified or authorized persons.
Storage stability
Recommended storage temperature
2 - 8 °C
Light sensitive. Store under inert gas.
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated

SECTION 8: Exposure controls/personal protection

8.1 Control parameters
Ingredients with workplace control parameters
8.2 Exposure controls

Personal protective equipment

Eye/face protection
Use equipment for eye protection tested and approved under appropriate
government standards such as NIOSH (US) or EN 166(EU). Safety glasses
Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove
removal technique (without touching glove's outer surface) to avoid skin contact
with this product. Dispose of contaminated gloves after use in accordance with
applicable laws and good laboratory practices. Wash and dry hands.
The selected protective gloves have to satisfy the specifications of Regulation (EU)
2016/425 and the standard EN 374 derived from it.
Full contact
Material: Nitrile rubber
Minimum layer thickness: 0,11 mm
Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0,11 mm

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 Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)
data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 87300, e-mail
sales@kcl.de, test method: EN374
If used in solution, or mixed with other substances, and under conditions which
differ from EN 374, contact the supplier of the EC approved gloves. This
recommendation is advisory only and must be evaluated by an industrial hygienist
and safety officer familiar with the specific situation of anticipated use by our
customers. It should not be construed as offering an approval for any specific use
scenario.
Body Protection
protective clothing
Respiratory protection
required when vapours/aerosols are generated.
Our recommendations on filtering respiratory protection are based on the following
standards: DIN EN 143, DIN 14387 and other accompanying standards relating to
the used respiratory protection system.
Recommended Filter type: Filter type ABEK
The entrepeneur has to ensure that maintenance, cleaning and testing of respiratory
protective devices are carried out according to the instructions of the producer.
These measures have to be properly documented.
Control of environmental exposure
Do not let product enter drains.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
a) Appearance Form: liquid
b) Odor No data available
c) Odor Threshold No data available
d) pH No data available
e) Melting No data available
point/freezing point
f) Initial boiling point No data available
and boiling range
g) Flash point No data available
h) Evaporation rate No data available
i) Flammability (solid, No data available
gas)
j) Upper/lower No data available
flammability or
explosive limits
k) Vapor pressure No data available
l) Vapor density No data available
m) Density 1,0054 g/cm3 at 20 °C

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 Relative density No data available
n) Water solubility at 20 °C soluble
o) Partition coefficient: No data available
n-octanol/water
p) Autoignition No data available
temperature
q) Decomposition No data available
temperature
r) Viscosity Viscosity, kinematic: No data available
Viscosity, dynamic: No data available
s) Explosive properties No data available
t) Oxidizing properties No data available
9.2 Other safety information
No data available

SECTION 10: Stability and reactivity

10.1 Reactivity
No data available
10.2 Chemical stability
The product is chemically stable under standard ambient conditions (room temperature) .
10.3 Possibility of hazardous reactions
No data available
10.4 Conditions to avoid
no information available
10.5 Incompatible materials
Acids, Bases, Oxidizing agents, Reducing agents, Iron and iron salts., Copper, Aluminum,
Brass, Free radical initiators
10.6 Hazardous decomposition products
In the event of fire: see section 5

SECTION 11: Toxicological information

11.1 Information on toxicological effects
Mixture
Acute toxicity
Oral: No data available
Acute toxicity estimate Oral - 455,6 mg/kg
(Calculation method)
Symptoms: Irritations of mucous membranes in the mouth, pharynx, oesophagus and
gastrointestinal tract.
LC50 Inhalation - 4 h - 11 mg/l
Symptoms: Possible symptoms:, mucosal irritations

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 Acute toxicity estimate Dermal - > 2.000 mg/kg
(Calculation method)
Skin corrosion/irritation
Mixture causes skin irritation.
Serious eye damage/eye irritation
Mixture causes serious eye irritation.
Respiratory or skin sensitization
May cause sensitization by skin contact.
Mixture may cause an allergic skin reaction.
Germ cell mutagenicity
Possible mutagen
Carcinogenicity
No data available
Reproductive toxicity
Evidence to impair fertility.
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
Mixture causes damage to organs through prolonged or repeated exposure. - Peripheral
nervous system
Aspiration hazard
No data available
11.2 Additional Information
Acrylamide toxicity is manifested as a sensorimotor peripheral neuropathy.
Other dangerous properties can not be excluded.

This substance should be handled with particular care.

Handle in accordance with good industrial hygiene and safety practice.

Components
acrylamide
Acute toxicity
LD50 Oral - Rat - female - 177 mg/kg
(OECD Test Guideline 401)
Acute toxicity estimate Inhalation - 1,6 mg/l
(Expert judgment)
LD50 Dermal - Rabbit - male and female - 1.141 mg/kg
(OECD Test Guideline 402)
Remarks: (Regulation (EC) No 1272/2008, Annex VI)
Skin corrosion/irritation
Causes skin irritation. (Regulation (EC) No 1272/2008, Annex VI)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Eye irritation - 24 h

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 (OECD Test Guideline 405)
(Regulation (EC) No 1272/2008, Annex VI)
Respiratory or skin sensitization
Maximization Test - Guinea pig
Result: positive
(OECD Test Guideline 406)
Remarks: (Regulation (EC) No 1272/2008, Annex VI)
Germ cell mutagenicity
May cause genetic defects.
Test Type: Ames test
Test system: Salmonella typhimurium
Result: negative
Test Type: Chromosome aberration test in vitro
Test system: Chinese hamster lung cells
Result: positive
Test Type: In vitro mammalian cell gene mutation test
Test system: Chinese hamster ovary cells
Result: negative
Method: OECD Test Guideline 478
Species: Rat - male
Result: positive
Carcinogenicity
Presumed to have carcinogenic potential for humans
Reproductive toxicity
Suspected of damaging fertility.
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
Oral - Causes damage to organs through prolonged or repeated exposure. -
Peripheral nervous system
Aspiration hazard
No data available

N,N'-methylenediacrylamide
Acute toxicity
LD50 Oral - Rat - female - 50 - 300 mg/kg
(OECD Test Guideline 423)
Inhalation: No data available
Dermal: No data available
Skin corrosion/irritation
No data available
Serious eye damage/eye irritation
No data available
Respiratory or skin sensitization
No data available
Local lymph node assay (LLNA) - Mouse

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 Result: negative
(OECD Test Guideline 442B)
Germ cell mutagenicity
May cause genetic defects.
Test Type: Ames test
Test system: Salmonella typhimurium
Result: positive
Remarks: (National Toxicology Program)
Method: OECD Test Guideline 478
Species: Rat - male
Result: positive

Result: positive
Remarks: The value is given based on a SAR/AAR approach using OECD Toolbox,
DEREK, VEGA QSAR models (CAESAR models), etc.
Carcinogenicity
No data available
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available

SECTION 12: Ecological information

12.1 Toxicity
Mixture
No data available
12.2 Persistence and degradability
No data available
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
This substance/mixture contains no components considered to be either persistent,
bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at
levels of 0.1% or higher.
12.6 Other adverse effects
No data available
Components
acrylamide
Toxicity to fish static test LC50 - Oncorhynchus mykiss (rainbow trout) - 180

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 mg/l - 96 h
(OECD Test Guideline 203)
Toxicity to daphnia flow-through test EC50 - Daphnia magna (Water flea) - 98 mg/l
and other aquatic - 48 h
invertebrates (US-EPA)
Toxicity to algae static test NOEC - Pseudokirchneriella subcapitata - 56 mg/l -
72 h
(OECD Test Guideline 201)
Toxicity to bacteria EC50 - Photobacterium phosphoreum - 13.500 mg/l
Remarks: (IUCLID)

N,N'-methylenediacrylamide
Toxicity to fish LC50 - Danio rerio (zebra fish) - > 100 mg/l - 96 h
(OECD Test Guideline 203)
NOEC - Danio rerio (zebra fish) - 100 mg/l - 96 h
(OECD Test Guideline 203)

SECTION 13: Disposal considerations

13.1 Waste treatment methods
Product
See www.retrologistik.com for processes regarding the return of chemicals and
containers, or contact us there if you have further questions.

SECTION 14: Transport information

14.1 UN number
ADR/RID: 3426 IMDG: 3426 IATA: 3426
14.2 UN proper shipping name
ADR/RID: ACRYLAMIDE SOLUTION
IMDG: ACRYLAMIDE SOLUTION
IATA: Acrylamide solution
14.3 Transport hazard class(es)
ADR/RID: 6.1 IMDG: 6.1 IATA: 6.1
14.4 Packaging group
ADR/RID: III IMDG: III IATA: III
14.5 Environmental hazards
ADR/RID: no IMDG Marine pollutant: no IATA: no
14.6 Special precautions for user
No data available

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the
substance or mixture

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 This material safety data sheet complies with the requirements of Regulation (EC) No.
1907/2006.
Authorisations and/or restrictions on use
REACH - Candidate List of Substances of Very : acrylamide
High Concern for Authorisation (Article 59).
REACH - Restrictions on the manufacture, : acrylamide
placing on the market and use of certain
dangerous substances, preparations and articles
(Annex XVII)

Other regulations
Observe work restrictions regarding maternity protection in accordance to Dir 92/85/EEC or
stricter national regulations where applicable.
Take note of Dir 94/33/EC on the protection of young people at work.
15.2 Chemical Safety Assessment
For this product a chemical safety assessment was not carried out

SECTION 16: Other information

Full text of H-Statements referred to under sections 2 and 3.
H301 Toxic if swallowed.
H302 Harmful if swallowed.
H302 + H332 Harmful if swallowed or if inhaled.
H312 Harmful in contact with skin.
H315 Causes skin irritation.
H317 May cause an allergic skin reaction.
H319 Causes serious eye irritation.
H332 Harmful if inhaled.
H340 May cause genetic defects.
H350 May cause cancer.
H361f Suspected of damaging fertility.
H372 Causes damage to organs through prolonged or repeated exposure if
swallowed.
Further information
The above information is believed to be correct but does not purport to be all inclusive
and shall be used only as a guide. The information in this document is based on the
present state of our knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any guarantee of the properties of
the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any
damage resulting from handling or from contact with the above product. See
www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional
terms and conditions of sale.

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