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Scientific Limited

Instruction manual

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IMPORTANT Read before using

• Check the Centrifuge and the Packaging for any shipping damage.
Inform the shipping company and Centurion Scientific immediately
• Check the order for completeness, if not contact Centurion Scientific

Location
The Centrifuge should only be operated indoors.
The set up location must fulfil the following requirements
• A safety zone of at least 30Cm must be maintained around the Centrifuge
• People and hazardous substances must be kept out of this zone
during centrifugation
• The bench or supporting structure must be stable and free from resonance
• The Bench or supporting structure must be suitable for horizontal use of the
centrifuge
• The Centrifuge should Not be exposed to heat or strong sunlight
• Ultra violet rays can damage the stability of plastics and coatings
• Do not subject the Polypropylene, Delrin rotors or accessories to direct
sunlight
• The Zone location must be well ventilated at all times

• Store the original packaging, Keep this for future service to base , if required.

Transporting the Centrifuge


• Always remove the Rotor before transportation
• Due to its weight the Centrifuge should be carried by several people
• Transport the Centrifuge upright and if possible in its packaging
• Always lift the centrifuge at both sides (see below). NOT front to rear.

2
Fixing of rotor

Fixing for micro and fixed angle rotors


BRK5401. BRK54XXseries. BRK53XX series. BRK52XX series

Locate 3 pins of fixing,into 3 holesat the top of the rotor


Push down firmly into holes.
Lift and fit rotor onto the motor shaft. M
Fit the Screw and spring washer, screw down tight with
the supplied tool.
Checktightness regularly.

Fixing for Swing out rotors.


BRK5508, BRK5506, BRK1001, BRK3000, BRK5540, BRK5740.

Motor shaft

Lift and fit rotor onto the motor shaft.M


Fit the Screw and spring washer, screw down tight with the
supplied tool.
Check tightness regularly

3
Rotor balancing. Very Important

Balancing of tubes for Micro and Fixed angle rotors


Tare (zero) your scales first

Weigh a tube , note its


weight Weigh another and
match its weight to within 0.1
gram

Swing out rotor, buckets. Balancing


Place all your required tubes in all 4 buckets.
Equal amount per bucket.
Weigh each bucket containing tubes

Match other 3 buckets, with tubes to within 1


gram of each other

For BRK5506 rotor use the adaptor

4
Tube placement micro rotors

Haematocrit Rotor

Always fit the lid supplied, as shown to the left

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram. For best separations. (see page 4)
ALWAYS fit the lid supplied
CHECK the Rim seal gasket and replace every 2 months (Part number 8011)
CHECK the rotor screw fixing tightness regularly

...........................................................................................................................................................................

Micro tube Rotor

Always fit the lid supplied, as shown to the left

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram For best separations. (seep page 4)
ALWAYS fit the lid supplied
CHECK the two top seals and replace regularly (Part number 8044)
CHECK the rotor screw fixing tightness regularly 5
Tube placement Fixed angle rotors

Small fixed angle Rotors

Always fit the lid supplied, as shown to the left

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1 Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly

...................................................................................................................................................................................

Large fixed angle Rotors

Always fit the lid supplied, as shown to the left

✔ ✘ ✘

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1 Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly 6
Tube & bucket placements Swing out rotors

Small Swing out rotor

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1 Gram. For best separations
ALWAYS fit ALL the Tube Holders and any supports
CHECK The rotor screw fixing tightness regularly

.................................................................................................................................................................................

Medium – Large Swing out Rotors

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS Weigh Bucket and all samples within to 1.0 Gram
ALWAYS fit ALL 4 Buckets and 4 Adaptors supplied
CHECK the rotor screw fixing tightness regularly

.................................................................................................................................................................................

Microtitor Rotor

✔ ✘
ALWAYS load your plates opposite to each other
ALWAYS weigh samples to within 1.0 Grams. For best separations
ALWAYS fit both Buckets supplied (2)
CHECK the rotor screw fixing tightness regularly

7
Tube placements Swing out rotor

Wrong

Wrong

This is not balanced!

Even though there are two samples in two buckets opposite to each other it is not balanced.
NEVER run swing out rotors with only two buckets

All four buckets must be used at all times

Always use the same size tubes as in A


per bucket.

NEVER use different size tubes as in B


per bucket
Check they fit and do not hit the
centre as shown in C

8
A B
Tube placement Cytology & Cell wash rotors

Cytology Rotors

Always fit the lid supplied, as shown below

✔ ✘ ✘
ALWAYS load your holders opposite to each other
ALWAYS weigh samples to within 0.5 Gram. For best separations
ALWAYS fit the lid supplied
Check the two top Gaskets and replace regularly (Part Number 8055)
CHECK the rotor screw fixing tightness regularly

................................................................................................................................................................................

Manual Cell W ashing Rotor

✔ ✘ ✘
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.2 Gram. For best separations Regularly smear a small amount of Silicon grease over the O rings
Check the inner and outer O rings and replace regularly (Part Number 8066)
CHECK the rotor screw fixing tightness regularly
9
Index. Instruction manuals for.
Micro's

EZ913 Haematocrit Centrifuge. Complete with BRK5401 rotor


24 x Capillary tubes & 12 x 2ml tubes

EZ904 Swing out Rotor Centrifuge. Complete with BRK5508S rotor

8 x 5ml Blood tubes

EZ915 Micro tube Centrifuge. Complete with BRK5424 (A), BRK5436 (B),
BRK5448 (C), BRK5494 (D)

EZ 906 Fixed angle rotor Centrifuge. Complete with BRK5408 (A), BRK5404 (B)

...........................................................................................................................................................

Small to .5Litre

EZ704 Swing out rotor Centrifuge. Complete with BRK5506 rotor

EZ706 Fixed angle rotor Centrifuge. Complete with BRK5412 (A), BRK5406 (B) rotor

..........................................................................................................................................................
Medium to 1Litre
EZ504 Swing out rotor Centrifuge. Complete with BRK1001 rotor & B5250 Bckts

EZ506 Fixed angle rotor Centrifuge. Complete with BRK5324 (A), BRK5308 (B)
Instructions of use

Program Fault

1 Lid open

2 Imbalance

3 None revolve

4 Inverter

Start.
Stop Function Lid open

................................................................................................................................................................................

Program See following pages for analysis methods per model

1 By pressing Function button you select any


One of four programs, each light up accordingly
2 Actual details are shown in this manual per model

3
Once running, your selected program will flash
4 When the run is finished it will flash faster

Function

Once your program has been selected, load your tubes, Start.
Stop
evenly and same weight, close the lid and press start.
Selected program light will Flash whilst running, when
finished A buzzer will sound (can be switched off)

Press retrieve your samples.


Lid open
Fault codes

How to turn the Buzzer off.

Press Function and Start stop.


Top Fault LID will flash when Buzzer is enabled
Bottom fault LID will flash when disabled

Fault Codes. Orange coloured displays

Fault

Lid open. Close lid firmly (check that nothing is fouling closure)

If problem persists contact Service

Imbalance An imbalance has occurred, load samples opposite and evenly


Weigh your samples to match, clean your rotor.
If problem persists contact Service

None revolve Rotor will not turn, open lid, check if it turns freely, are your tubes
too tall?
If problem persists contact Service

Inverter A fault has occurred. Turn off your Centrifuge wait 3 minutes
Turn on again.
If problem persists contact Service
Programs per Model. Micro's

EZ913 1 2 3 4

Program PCV Serum 1 Serum 2 Urine


Speed (Rpm) 13,000 6,000 5,000 2,100
Time (Seconds) 300 240 300 600
Accelleration 9 8 8 8
Deceleration 9 8 8 8
Rcf (g) 15,870 3380 2348 414
Rotor. BRK5401 Haematocrit/ 12 x 2ml

EZ904 1 2 3 4
Program Serum 1 Serum 2 Urine Sperm
Speed (Rpm) 4,000 4,000 2,100 3,300
Time (Seconds) 420 600 600 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8

Rcf (g) 1520 1520 419 1034


Rotor. BRK5508S 8 x 5ml Swing out

EZ915
1 2 3 4
Program 15 mins 10 mins 5 mins Pellet
Speed(Rpm) 15,000 15,000 15,000 15,000
Time (Seconds) 900 600 300 30
Accelleration 9 8 8 9
Deceleration 9 8 8 9

Rcf (g) 22,000 22,000 22,000 22,000


Rotor.BRK5424 (A), BRK5436 (B), BRK5448 (C), BRK5494 (D)

EZ906
Program Serum Faeces Urine Sperm
Speed(Rpm) 6,000 1,780 2,080 3,250
Time (seconds) 300 300 600 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8
Rcf(g) 3421 301 411 1004
Rotor. BRK5408 (A), BRK5404 (B)
Programs per Model Small

EZ704 1 2 3 4

Program Speed Serum fast Faeces Urine Sperm


(Rpm) Time 4,000 1480 1700 2700
(Seconds) 300 300 600 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8
Rcf(g) 2235 314 404 1020
Rotor BRK5506 & 1 set of adaptors

EZ706 1 2 3 4

Program Serum 1 Faeces Urine Sperm


Speed (Rpm) 6,000 1700 1950 3100
Time (Seconds) 300 300 600 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8
Rcf(g) 3820 310 400 1020

Rotor BRK5412 (A), BRK5406 (B)


Programs per Model Medium

EZ504
1 2 3 4
Program Serum Faeces Urine Sperm
Speed(Rpm) 4,000 1400 1600 2,500
Time (Seconds) 300 600 300 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8

Rcf (g) 2611 320 420 1020


Rotor BRK1001, B5250 buckets & 1 set of adaptors

EZ506 1 2 3 4
Program Serum Faeces Urine Sperm
Speed(Rpm) 5,000 1,500 1,750 2,750
Time (seconds) 240 600 300 900
Accelleration 9 8 8 8
Deceleration 9 8 8 8
Rcf(g) 3350 301 411 1010
Rotor BRK5324, (A) BRK5308 (B).rotor
Fault codes
Prime and Dual models only. Ambient
Fault codes are shown in the Speed section of the LED display (shown on previous page

The following List show what each Code means and what to do.

FA50 Inverter Drive Look at the Obround viewing port (Rear)


Note the Code shown in the inverter display. *

Turn off the power at the wall mains switch only.


WAIT 3 minutes, Turn on again and Run. If problem persists
Contact our Service Department.

FA21 Inverter error As above.

Obround viewing port shown. *

Non Fault Codes

Speed will not go beyond 2000 Rpm.


. The Electronics have set the Speed to safe mode
. Check the rotor bottom has magnets fitted

. Check correct rotor is in use against program chosen

. If not then Contact our Service Department

Noisy
. Unbalanced tubes, see Rotor pages
. Lid seal needs replacing
. Rotor Lid not fitted

Lid support fail


. Spring has failed. Contact our Service Department
Emergency Lid release
Mechanical Emergency Door release
During a power failure, you will not be able to open the Centrifuge lid via the Display button
Mechanical override is provided to allow sample recovery
However this is for Emergency use only
This should Not be used for normal use.

Warning
The rotor can still be spinning at a high speed, if touched it can cause serious injuries.
Always wait till the rotor has stopped.
Proceed as follows

• Make sure the Rotor has stopped (view via the port in the Lid)

• Once stopped turn off the power via the inlet Switch

• Pull the Centrifuge slightly over the bench (as shown in (a)

• You will notice one (b) or some models two plastic plug (c). Plug *
• Pop open with a screwdriver (tool) and pull downwards .

• The lid will pop open You can now retrieve your samples

b
a

c c

After replace the Plastic plugs back into their original position.
Reconnect the centrifuge power line once the mains power has been restored.
Check all is working correctly by closing the lid, wait 5 seconds then press lid open.
If not working refit or Contact your Distributor

16
Care & maintenance. Centrifuge

Always keep your Centrifuge clean and free from moisture at all
! times, this will help prevent corrosion.

Do not use any kind of scourers or abrasive material to clean the


! Centrifuge and ensure that a pH neutral cleaner is always used.

! It is recommended that your centrifuge is switched off and the lid left
open overnight to ensure that any moisture build up can evaporate.

Keep a 30Cm distance around your Centrifuge.


! Do not site the unit in sunlight

Always ensure that lids and accessories are correctly tightened


! before running the unit.

Use only official Centurion Scientific Ltd accessories with your centrifuge.
!

! It is recommended that regular maintenance and cleaning of the unit and


accessories is carried out.

! Yearly servicing is required to validate your warranty and increase the life
of your Centrifuge and accessories. See PrO-Care pages

10
Care & maintenance. Rotor

! Always check rotors for cracks and damage, if found replace


your rotor. DO NOT USE as dangerous

! Autoclave for the maximum number of times shown in the


sales literature. In brackets( 20)

! Rotors have a Seven year lifespan and must be replaced .

DO NOT USE beyond their Lifespan

! It is recommended that regular maintenance and cleaning of the rotor and


accessories is carried out.

Ensure that all buckets are present in the rotor when running your
! centrifuge, as using the unit with an unbalanced rotor can cause distortion
and excessive wear. Read tube placements section in the previous section

! Do not use any kind of scourers or abrasive material to clean the buckets
and ensure that a pH neutral cleaner is always used.

! Aluminium buckets should be kept clean and moisture free to keep them in a
good, safe operating condition.

! Yearly servicing is required to validate your warranty and increase the life
of your rotor and accessories

11
PrO-Care
Service, Warranty & Maintenance pakages

The lifespan of a centrifuge depends


upon how well it is looked after and
maintained.
Many of our centrifuges have lived a life
of over 15 years.

This is not only due to our stringent manufacturing process


and high quality components but also to a yearly servicing
package. Making them a cost effective solution of centrifugation.

Whilst our centrifuges come with a confidence giving


warranty, yearly services are imperative to ensure your warranty
is valid, this also ensures your machine is working to
its best ability and lengthens its life span. Also as centrifuges
are a dangerous machine, safety checks are required too.

For UK customers we offer a back to base service package


including carriage to us and return all within 48 hours. Centrifuge
seals , fuses & other parts are replaced,
adjustments made, rotor checked for stress cracks, seals changed
if necessary (extra charge) PAT (portable appliance test) is
included Obligatory in UK.

For worldwide customers we offer a full parts package per model.


The designated country distributor services the units accordingly.

Call our Office to discuss or visit our Service website

WWW.ServicePrO-Care.com

www.Centurionscientificglobal.com Switchboard. 0044 (0) 2392 631225

Research & service Ambient Refrigeration Sales


The Old equine Barn manufacturing Manufacturing The Old Stables Church
Church Farm The Old Grain Barn The Old Drier Barn Farm Stoughton
Stoughton Chichester Chichester
Church Farm Church Farm
PO18 9JL. UK Stoughton Chichester Stoughton
PO18 9JL. UK Chichester
PO18 9JL. UK

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