Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 0 views

    hematopoiesis.dea.rppt

    Uploaded by

    Thalitha Aurelia

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    hematopoiesis.dea.rppt

    Uploaded by

    Thalitha Aurelia
    0 views36 pages

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    0 views36 pages

    hematopoiesis.dea.rppt

    Uploaded by

    Thalitha Aurelia

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 36

    HISTOLOGY DEPARTMENT

    MEDICAL FACULTY
    LAMPUNG UNIVERSITY
    HEMATOPOIESIS
    (Haima : blood , poiesis : a making)

    proliferation & differentiation from stem cells

    Devided into :
     Erythropoiesis
     Granulopoiesis
     Monocytopoiesis
     Megakaryocytopoiesis / thrombopoiesis
     Lymphopoiesis
    Extra embryonic mesoderm of the yolk sac

    Fetal liver & Spleen

    Bone marrow of the clavicle

    Bone marrow of the skeletons


    A. Hematopoietic Stem Cells
    Stem cells : pluripotential cells capable of
    self-renewal, low mitotic activity
     Form specific irreversible
    differentiated cells types
     Remain stem cell

    Progenitor cells : unipotential cells, high


    mitotic activity
    Precursor cells (Blast) : morphologic
    characteristics differentiate for the first
    time

    Colony-forming cells (CFC) or


    Colony-forming units (CFU)
    Gambar!!!!!!!!

    Differentiation of pluripotential and multipotential cells


    during hematopoiesis.
    Growth factors :
    Colony-stimulating factors (CSF) or
    Hematopoietins (Poietins)

    Functions :
    Stimulating proliferation of immature cells
    Supporting the differentiation of maturing
    cells
    Enhancing the functions of mature cell
    Releasing factors :
    Control the release of mature bone cells
    from bone marrow

    C3 component of complement
    Hormones (Glucocorticoids & androgens)
    Bacterial toxins
    B. Bone Marrow

    In medullary canals of long bones &


    cavities of cancellous bone
    Types :
    1. Red or Hematogenous bone marrow:
    presence of blood & blood forming
    cells
    2. Yellow bone marrow : presence of
    adipose cells
    Red Bone Marrow

    Function :
    Production of blood cells
    Destruction of red blood cells
    Storage of iron derived
    Composed of :
    1. Stroma : reticullar cells,reticular fiber &
    hematopoietic cells
    2. Hematopoietic cords / matrix : collagens
    types II – III, fibronectin, laminin &
    proteoglicans
    3. Sinusoidal capillary : endothelial cells +
    layer of reticular cells & fiber
    C. Maturation of Erythrocytes
    Cellular changes :
    Cell volume decrease
    Nucleoli diminish in size
    Nuclear diameter decrease
    Chromatin more dense
    Pycnotic of nucleus extruded
    Decrease in the number of polyribosome
    Increase in the amount of hemoglobin
    Mitochondria disappear
    Needs :
    Erythropoietin
    Iron
    Folic acid
    Vitamin B12
    Erythropoiesis

    P : Proeritroblast
    N1 : Basophilic Erythroblast
    N2 : Polychromatophilic
    Erythroblast
    N3 : Orthochromatophilic
    Erythroblast
    I. Proerythroblast
    Large cells
    Loose, lacy chromatin
    Clearly visible nucleoli

    II. Basophilic Erythroblast

    Basophilic cytoplasm polyribosomes


    Condensed nucleus
    III. Polychromatophilic Erythroblast
    Cytoplasm hemoglobin

    IV. Orthochromatophilic Erythroblast


    or Normoblast

    Condense nucleus
    Acidophilic cytoplasm
    V. Reticulocyte
    Expels of nucleus
    Polyribosomes +

    VI. Erythrocyte

    Loses of polyribosome
    D. Maturation of Granulocytes
    11 days
    5 mitotic division

    M1 : Myelocyte
    M2 : Metamyelocyte
    M3 : Stab cell
    M4 : Mature neutrophil
    I. Myeloblast
    Dispersed chromatin
    Nucleoli +

    II. Promyelocyte
    Basophilic cytoplasm
    Azurophilic granule
    III. Myelocyte

     Condensation nucleus
    Neutrophilic myelocyte
     Increase specific granule Basophilic myelocyte
    Eosinophilic myelocyte

    IV. Metamyelocyte
    Kidney-shape nucleus
    Condensation nucleus
    V. Band cell
    Band-shape nucleus
    Mature granule

    VI. Neutrophil, Basophil & Eosinophil


    Lobulated nucleus
    Rough chromatin
    Mature granule loaded cytoplasm
    Kinetics of neutrophil production

    1. The medullary formation


    compartments
    Mitotic compartment (3 days)
    Maturation compartment (4 days)

    2. A medullar storage compartment


    Buffer system (4 days)
    3. The circulating compartment
    In plasma & circulating in
    6-7
    blood vessel hours

    4. The marginating compartment


    In blood vessel but not circulate

    5. Connecting tissue
    Phagocytosis (1-4 days)
    Bone marrow
    Mitosis Maturation
    Stem cell Metamyelocyte
    Myeloblast Band cell 1
    Promyelocyte Mature granulocyte
    Myelocyte

    Storage 2

    Blood

    4 Marginating Circulating
    3
    cell cell

    Migration into Migration to, & death


    Natural cavities In the connecting tissue
    E. Maturation of Lymphocyte &
    Monocyte
    Distinguished basis on :
    Size
    Chromatin structure
    Presence of nucleoli
    Immunocytochemical technique
    cell-surface receptors
    Lymphocytes
    Bone marrow

    Circulation

    B lymphocytes
    Thymus
    T lymphocytes

    Peripheral Spleen
    lymphoid Lymph nodes
    organs Tonsils
    I. Lymphoblast
    Large cells
    3H-thymydine

    II. Prolymphocyte
    Smaller
    Condensed chromatin
    III. T & B Lymphocytes
    Cell-surface receptor
    Condensated nucleus
    Slightly cytoplasm
    Monocytes

    I. Monoblast

    Identical with myeloblast


    Dispersed chromatin
    Nucleoli +
    II. Promonocyte
    Large cell
    Basophilic cytoplasm
    Large, slightly indented nucleus
    Lacy chromatin
    Evident nucleoli
    III. Monocyte

    Rough endoplasmic reticulum +++


    Extensive golgi complex
    Granule

    Bloodstream Connective tissue


    8 hours

    macrophages
    Several months
    F. Trombopoiesis
    F. Trombopoiesis
    I. Megakaryoblast
    Ǿ 15 – 50 µm
    Large ovoid or kidney-shape nucleus
    Numerous nucleoli
    Cytoplasm : homogeneous & basophilic
    II. Megakaryocytes
    Giant cells, Ǿ 35 – 150 µm
    Irregularly lobulated nucleus
    Coarse chromatin
    No visible nucleoli
    Cytoplasm contains numerous :
    Mitochondria
    Rough endoplasmic reticulum
    Golgi complex
    III. Platelet
    10 days
    From invaginations the plasma membrane
    4000 – 8000 platelets
    Granule :
    Platelet-derived growth factor
    Fibroblast growth factor
    Von Willebrand’s factor
    Platelet factor IV

    You might also like