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    Mechanism of Enzyme Action

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    Rubi Ann
    This document discusses the mechanism of enzyme action. It begins with learning outcomes and prior knowledge about enzymes breaking down maltose into glucose. It then defines enzymes as biological catalysts that direct cellular reactions. The main part explains the "lock and key" mechanism - where the specific substrate binds to the active site of the enzyme, forming an unstable enzyme-substrate complex. This leads to product molecules leaving the active site, freeing the enzyme to catalyze another reaction. It provides examples of how sucrose can bind to sucrase but maltose cannot. Finally, it includes an assessment with multiple choice and short answer questions testing understanding of this mechanism.

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    © All Rights Reserved
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    Mechanism of Enzyme Action

    Uploaded by

    Rubi Ann
    143 views19 pages
    This document discusses the mechanism of enzyme action. It begins with learning outcomes and prior knowledge about enzymes breaking down maltose into glucose. It then defines enzymes as biological catalysts that direct cellular reactions. The main part explains the "lock and key" mechanism - where the specific substrate binds to the active site of the enzyme, forming an unstable enzyme-substrate complex. This leads to product molecules leaving the active site, freeing the enzyme to catalyze another reaction. It provides examples of how sucrose can bind to sucrase but maltose cannot. Finally, it includes an assessment with multiple choice and short answer questions testing understanding of this mechanism.

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    Mechanism of Enzyme Action

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    This document discusses the mechanism of enzyme action. It begins with learning outcomes and prior knowledge about enzymes breaking down maltose into glucose. It then defines enzymes as biological catalysts that direct cellular reactions. The main part explains the "lock and key" mechanism - where the specific substrate binds to the active site of the enzyme, forming an unstable enzyme-substrate complex. This leads to product molecules leaving the active site, freeing the enzyme to catalyze another reaction. It provides examples of how sucrose can bind to sucrase but maltose cannot. Finally, it includes an assessment with multiple choice and short answer questions testing understanding of this mechanism.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    143 views19 pages

    Mechanism of Enzyme Action

    Uploaded by

    Rubi Ann
    This document discusses the mechanism of enzyme action. It begins with learning outcomes and prior knowledge about enzymes breaking down maltose into glucose. It then defines enzymes as biological catalysts that direct cellular reactions. The main part explains the "lock and key" mechanism - where the specific substrate binds to the active site of the enzyme, forming an unstable enzyme-substrate complex. This leads to product molecules leaving the active site, freeing the enzyme to catalyze another reaction. It provides examples of how sucrose can bind to sucrase but maltose cannot. Finally, it includes an assessment with multiple choice and short answer questions testing understanding of this mechanism.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 19

    TOPIC :

    MECHANISM OF
    ENZYME ACTION

    CONTENT
    1

    LEARNING
    OUTCOME

    INTRODUCTION

    PRIOR
    KNOWLEDGE

    DEFINITION OF
    ENZYME

    MECHANISM OF
    ENZYME ACTION

    ASSESSMENT

    LEARNING OUTCOME

    4.10 :
    Explain the mechanism of
    enzyme action

    INTRODUCTION
    Do you realize the
    term of COENZYME
    on detergent
    products?

    PRIOR KNOWLEDGE
    RECALL !!!
    Maltose + Water

    Maltas
    e

    Glucose + Glucose

    ENZYME maltase BREAK


    DOWN maltose into 2
    molecules of glucose

    Prior knowledge
    Enzyme
    s

    Cloth

    Stains

    How the stains have been


    removed from the cloth?

    DEFINITION OF ENZYME

    Enzymes are biological


    catalyst that direct or guide
    almost all cellular reactions.

    MECHANISM OF ENZME
    ACTION
    LOCK AND KEY
    HYPOTHESIS

    MECHANISM OF ENZME
    ACTION
    Product

    Product
    Enzyme-substrate
    complex

    Enzyme

    EXPLANATIO
    N

    EXPLANATIO
    N

    Substrate

    EXPLANATIO
    N

    MECHANISM OF ENZME
    ACTION

    A specific substrate molecule


    arrives at the active site of the
    enzyme molecule.

    MECHANISM OF ENZME
    ACTION

    The substrate molecule binds to


    the active site to form an
    enzyme-substrate complex
    which is very unstable.

    MECHANISM OF ENZME
    ACTION
    Product molecules leave the
    active site of the enzyme.
    The enzyme is free to take
    another substrate molecule into
    its active site to catalyse
    another reaction.

    MECHANISM OF ENZME
    ACTION

    Which substrate can bind to active site of


    enzyme sucrase?
    Sucrose can bind to
    active site of sucrase

    Sucrose

    Sucrase

    Maltose

    Maltose CANNOT bind


    to active site of sucrase

    Enzymes
    have
    specific
    sites
    called
    active site
    which bind
    to specific
    substrates

    assessment

    Question 1

    Question 2

    Question 3

    Question 4

    Question 1
    Fill the blank with the correct answer.
    1. A specific ______________ molecule arrives
    at the active site of the ______________ molecule.
    2. The substrate molecule binds to the active site of the
    enzyme to form an ______________.

    Product
    enzymesubstrat
    e
    complex
    enzyme

    3. The enzyme is free to take another substrate molecule


    into its ______________ to catalyse another reaction.
    4. ______________ molecules leave the active site of the
    enzyme.

    active
    site
    substrat
    e

    Question 2

    Which of the following shows the characteristic of


    enzymes?
    A. Enzyme reaction is specific
    B. Enzyme reaction is influenced by pH
    C. Enzyme reaction is influence by
    temperature
    D. The structure of the enzyme change at
    the end of the reaction.

    Question 3
    Protea
    se

    Dissolve
    starch stains
    on cloth

    Amylas
    e

    Ripening
    cheese

    Lipase

    Dissolve
    protein stains
    on cloth

    Question 4
    Enzyme is used to wash away the blood stain on a
    shirt.
    Explain how the enzyme acts on the blood stain at a
    maximum rate.
    (3 MARKS)

    ANSWER :
    Blood is made up from proteins. Protease
    in detergent will break down protein into
    amino acids. This reaction occur

    THANK YOU .

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