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Skin Graft

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SKIN GRAFT

AUGUST 2018
ANATOMY
EPIDERMIS
• Stratified squamous epithelium
composed primarily of
keratinocytes.
• No blood vessels.
• Relies on diffusion from
underlying tissues.
• Separated from the dermis by a
basement membrane
DERMIS
Composed of two “sub-layers”:
• superficial papillary
• deep reticular.
The dermis contains collagen,
capillaries, elastic fibers,
fibroblasts, nerve endings, etc
DEFINITION
• skin graft is a tissue of epidermis and varying
amounts of dermis that is detached from its
own blood supply and placed in a new area
with a new blood supply. Graft Does not
maintain original blood supply.
• Does not maintain original blood supply.
CLASSIFICATION
1. Autografts – A tissue transferred from one
part of the body to another.
2. Homografts/Allograft – tissue transferred
from a genetically different individual of the
same species.
3. Xenografts – a graft transferred from an
individual of one species to an individual of
another species.
TYPES
• Split thickness Skin Graft
(STSG)
- Thin
- Intermediate
- Thick

• Full Thickness Skin Graft


(FTSG)
INDICATION
Split Thickness Used when cosmetic appearance
is not a primary issue or when the size of the
wound is too large to use a full thickness graft.

1. Chronic Ulcers
2. Temporary coverage
3. Correction of pigmentation disorders
4. Burns
Full Thickness Indications for full thickness skin
grafts include:

1. If adjacent tissue has premalignant or malignant


lesions and precludes the use of a flap.
2. 2. Specific locations that lend themselves well to
FTSGs include the nasal tip, helical rim,
forehead, eyelids, medial canthus, concha, and
digits.
Donor Sites of Skin Graft
split-thickness skin grafts
• the original donor site may be
used again for a subsequent split-
thickness skin graft harvest.

Full-thickness skin graft donor sites


• must be closed primarily because
there are no remaining epithelial
structures to provide re-
epithelialization.
Type of graft Advantages Disadvantages
Thin Split Thickness Best Survival - Least resembles original
Heals Rapidly skin
- Least resistance to
trauma.
- Poor Sensation
- Maximal Secondary
Contraction
Thick Split Thickness -More qualities of normal -Lower graft survival
skin. -Slower healing
-Less Contraction
-Looks better
-Fair Sensation
Full Thickness -Most resembles normal -Poorest survival.
skin. -Donor site must be closed
-Minimal Secondary surgically.
contraction -Donor sites are limited.
-Aesthetically pleasing
Recipient site preparation
Clean site after excision
Adequate hemostasis  Graft
Inadequate hemostasis  Delayed graft

Open wound with granulation tissue:


Suspected Infection Vascular supply
Should be removed before do a new graft
INSTRUMENT
1. Split thickness skin graft
- Humby knife
- Padgette Drum-Type
Dermatome
- Brown – Electrical
Dermatome
- Mesher
2. Full thickness skin graft
PROCEDURE STSG
PROCEDURE FTSG
Donor sites care
Split-Thickness Skin Grafts
- Concepts : Close wound + Keep moisture
- Dressing with Tulle Gras, Gauze and Bandage
- Alternative : Opsite, Duoderm, Cutinova
- Open dressing after 2 weeks for complete
epithelialization except suspected infection

Full -Thickness Skin Grafts


- No spontaneous healing  primary closure
Healing Process of Skin Grafts
1. Imbibition :
- during the first 24-48 hrs.
- place skin graft  vascularization
- temporary ischemia
- diffusion of nutrients by capillary action from the recipient bed (plasma +
RBC)

2. Inosculation :
- vessels in graft connect with those in recipient bed

3. Neovascularitation :
- graft revascularized by ingrowth of new vessels into bed - complete within 3-
5 days
Condition for Take of Skin Grafts
1. Close contact:
- Well vascularization
- Interrupted by tension, hematoma, seroma, pus

2. Immobilization :
-Tie-Over Bolus Dressing  5 days

3. Good blood supply of recipient area:


good blood supply : muscle, periosteum, perichondrium, paratendon
poor blood supply : bone cartilage, tendon

4. Infection
Skin Graft Storage
Used in Delayed Grafts / Skin Allografts
Already cutted skin can be stored by
1. Place back into donor site (10 days)
2. Wrap in NSS guaze and store in 4 °C (21 days)
3. Frozen and store in Skin Bank (5 years)
Composite Grafts
• Small graft containing skin and underlying cartilage or other
tissue
• Vascularization by Bridging phenomenon
• Distant between wound rim and graft < 0.5cm • Example : ear
skin and cartilage to reconstruct nasal alar rim defects,
Chondromucosal grafts from Nasal Septum to reconstruct lower
inner eyelid
THANK YOU

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