Production of Enzymes
Production of Enzymes
Production of Enzymes
Learning objective:
• To be able to explain why it is more
efficient to use isolated enzymes than
whole cells.
• To be able to explain how
contamination of end products is
eradicated.
• To be able to describe how this
process occurs.
Uses of enzymes
• commercial uses- e.g. biological
washing powders
• medical uses- e.g. biosensors,
therapeutic enzymes, drugs
• industrial uses - e.g. bioconversion,
fruit juice extraction, sweeteners
Enzymes are being used more and more for
industrial bioconversion i.e. making a
chemical product using purified enzymes
rather by pure chemical methods (e.g. citric
acid production) or using whole cells (e.g.
yeast in brewing).
• Considerations when selecting a strain:
– Does it do what is required
– Is it safe
– Is it cost effective
• Enzymes may be intracellular or extracellular.
What is the advantage of extracellular
production?
– Already outside cell
– Limited number secreted so easier to isolate
– More robust so less likely to be broken
down by heat of chemicals
• Why are intracellular enzymes more difficult to
isolate than extracellular ones?
• Because they are inside the cell, first the cell has to
be broken open then the enzyme separated from
the mixture of all the cellular contents
Enzyme in
solution