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    Applications of High Performance Liquid Chromatography (HPLC) in To Inorganic and Organometallic Compounds

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    Tuba Ahmed
    This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

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    Download as PPTX, PDF, TXT or read online from Scribd

    Applications of High Performance Liquid Chromatography (HPLC) in To Inorganic and Organometallic Compounds

    Uploaded by

    Tuba Ahmed
    201 views16 pages
    This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

    Original Description:

    Determine the working and principle of high pressure liquid chromatography

    Original Title

    HPLC

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    This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    201 views16 pages

    Applications of High Performance Liquid Chromatography (HPLC) in To Inorganic and Organometallic Compounds

    Uploaded by

    Tuba Ahmed
    This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

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    Applications of High performance

    liquid chromatography (HPLC) in


    to inorganic and organometallic
    compounds
    CHM-4191/631
    Course Title: Spectroscopy and chromatography
    Course Incharge: Dr. Ishrat Jamil
    Lecture content
     Introduction to HPLC
     Components of HPLC
     Working of the components of HPLC
     Block diagram of HPLC with the explaination

    of the separation process


     Applications of HPLC
    Objectives/learning outcomes
     At the end of this unit, you should be able to-
     Identify HPLC analysis
     Describe the function of the various

    components of a HPLC
     Application of HPLC for inorganic and organo

    metallic compounds
    Introduction to High performance
    liquid chromatography (HPLC)
    Components of HPLC
    Pump
     The role of the pump is to force a mobile
    phase through the liquid chromatograph at a
    specific flow rate, expressed in mL /min
     Normal flow rates in HPLC are in the 1-to 2-

    mL/min range.
     Typical pumps can reach pressures in the

    range of 6000-9000 psi (400-to 600-bar).


    Injector
     The injector serves to introduce the liquid
    sample into the flow stream of the mobile
    phase.
     Typical sample volumes are 5-to 20-

    microliters (μL).
     The injector must also be able to withstand

    the high pressures of the liquid system.


     An auto sampler is the automatic version for

    when the user has many samples to analyze


    or when manual injection is not practical
    column
     Considered the “heart of the chromatograph”
    the column’s stationary phase separates the
    sample components of interest using various
    physical and chemical parameters.
     The small particles inside the column are what

    cause the high back pressure at normal flow


    rates.
     The pump must push hard to move the mobile

    phase through the column and this resistance


    causes a high pressure within the
    chromatograph.
    Detector
     The detector can detect the individual
    molecules that come out from the column.
     A detector serves to measure the amount of

    those molecules so that the chemist can


    quantitatively analyze the sample
    components.
     The detector provides an output to a recorder

    or computer that results in the liquid


    chromatogram(i.e., the graph of the detector
    response).
    Computer
     Frequently called the data system, The
    computer takes the signal from the detector
    and uses it to
     determine the time of elution (retention time)

    of the sample components (qualitative


    analysis) and
     the amount of sample ( quantitative analysis)

    .
    Block diagram for HPLC
    Short summary of the components
    Cont..
    APPLICATIONS
    References and further reading

     Skoog, Holler, and Neiman. Principles of Instrumental


    Analysis. 5 th ed. Orlando: Harcourt Brace & Co., 1998.
     Handbook of HPLC [electronic resource] / edited by Katz,
    New York: M. Dekker, 1998.

     http://192.215.107.101/ebn/942/tech/techfoc
    us/1071main.html
     Introduction to Modern Liquid Chromatography,
    Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan, 3rd
    Ed Wiley; 3 edition ,December 9, 2009.
     Interactive secession will be conducted from 10.00am to

    3.00pm on Lms discussion Forum and 24/7 on whatsapp.

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