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FINFISH BREEDING AND HATCHERY
MANAGEMENT
Unit 1. Freshwater and marine fish seed resources and natural breeding
Freshwater fish seed resources
 Rivers were the major source of freshwater fish seed in India during 1950’s and 1960’s.
 Over the years, the riverine contribution has declined and at present forms only a
supplementary source, constituting less than 5% of the country’s total fish seed production.
 The Ganga, the Brahmaputra and the Indus river systems in the North and the Peninsular East
coast and the West coast river systems in the South are the important natural sources of fish
seed.
1.1.1. The Ganga river system
 The Ganges river system supports diverse freshwater fish fauna ranging from the cultivable
Gangetic (major) carps to mahseers and other coldwater fishes of the Himalayas The other
resources includes hilsa, catfishes and scampi (Macrobrachium rosenbergii) are of
considerable commercial importance.
The Principal freshwater fishery resources of the Ganga :
Indian major carps: Mahseer:
Catla catla Tor putitora
Labeo rohita T. mosal
Cirrhinus mrigala T. tor
Labeo calbasu Acrossocheilus hexagonolepis
Other carps: Larger catfishes:
Labeo pangusia Osteobagrus aor
L. dero O. seenghala
L .gonius Silonia silondia
Cirrhinus reba Wallago attu
Pangasius pangasius
Other catfishes: Bagarius bagarius
Clupisoma garva Rita rita
Eutropiichthys vacha Feather backs:
Ailia coila Notopterus notopterus
Ompok bimaculatus N. chitala
O. pabda
Clupeids: Freshwater prawns:
Hilsa ilisha Macrobrachium malcolmsoni
Gudusia chapra M. birmanicum
Setipinna phasa M. lamarei
1.1.2. The Brahmaputra system
The middle and lower stretches have several species of carps, catfishes and air breathing
fishes and also the anadromous hilsa.
The Principal freshwater fishery resources of the Brahmaputra :
Major carps: Catfishes:
Catla catla Silonia silondia
Labeo rohita Osteobagrus aor
Cirrhinus mrigala O. cavasius
Labeo calbasu Bagarius bagarius
Other cyprinids: Mahseers:
Labeo gonius Tor putitora
L. bata T. progenius
Danio equipinnatus Murrels:
Rasbora daniconius Channa punctatus
Puntius sarana C. marulius
P. ticto C. gachua
Barilius bendalensis Other fishes:
Chela atpar Rhinomugil corsula
Clupeids: Glassogobius giuris
Hilsa ilisha Colisa lalia
1.1.3. The Indus system
 The Indus river has the exotic rainbow trout and brown trout in the upper reaches and a
wide variety of indigenous carps and catfishes in the lower stretches.
 The trout streams of Kashmir constitute one of the world’s richest sport fishing waters
attracting anglers and tourists from world over.
• Harbors the exotic rainbow and brown trout, variety of indigenous carps and catfishes
• The trout streams of Kashmir - one of the world’s richest sport fishing
The Principal freshwater fishery resources of the Indus:
Carps: Catfishes:
Cyprinus carpio Glyptothorax kashmirensis
Schizothorax sp G.reticulatum
Oreinus lagiostomus Osteobagrus seenghala
Labeo dero
L. dyocheilus Other fishes:
Puntius conchonius Botia birdi
Crossocheilus latius Nemacheilus kashmirensis
Tor putitora N. rupicola
N. marmoratus
1.1.4. The East Coast system
The east coast river system in Peninsular constitutes the Mahanadi, the Godavari, the Krishna and the
Cauvery.
 The Mahanadi has similar resources as in Ganges system including all the Indian major carps.
 The other rivers, besides their own indigenous fish fauna of several carp species, catfishes,
murrels, prawns, etc. have had their water enriched by repeated transplantation of the
Gangetic carps from the North.
 The transplants have established themselves and contributed significantly to the fish fauna of
these rivers.
 The tributaries of the Cauvery from the Nilgris have coldwater fishes like trout and tench .
The Principal freshwater fishery resources of the Mahanadi:
Carps: Catfishes:
Catla catla Osteobagrus seenghala
L. rohita O. aor
C. mrigala Rita rita
L. fimbriatus R. chysea
Mahseers:
Tor mosal
The Principal freshwater fishery resources of the Godavari:
Carps: Catfishes:
• L. fimbriatus Osteobagrus seenghala
• C. mrigala O. aor
• L. calbasu Silonia childreni
• Catla catla Wallago attu
• L. rohita Pangasius pangasius
Bagarius bagarius
• Hilsa ilisha
• Macrobrachium malcolmsoni

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BREEDING AND CULTURE OF CARPS FISHES
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Carps form the mainstay of aquaculture in India contributing over 85% of the total aquaculture production There are 61,259 species of vertebrates recognized world; over 30,700 are fish species of which 8,411 ore fresh water while 11,650 are marine. In India 2,163 spp. are fin fishes have been recorded from upland cold water (157; 7.26%) warm water of the plain (54; 20.99%), Brackish water (182; 8.41%) and marine environment (1,370; 63.43%). Some of these species are cultured at commercial level which covering a lot varieties of fin fishes The three Indian major carps, namely Catla (Catla catla), Rohu (Labeo rohita) and Mrigal (Cirrhinus mrigal) contribute the bulk of production to the extent of 75 to85 percent of the total fresh water fish production, the three exotic carp such as Silver carp (Hypophthalmichthys molitrix) and Common carp (Cyprinus carpio ), Grass carp (Ctenopharyngodon idella )form the second important group to incorporate several other medium and minor carp and into the carp poly culture system several method were used because of their region specific consumer preference and higher market demand. History Carp culture in India was restricted only to a homestead backyard pond activity in west Bengal and Odisha until late 1950 s with seed from riverine sources as the only input resulting low level of production the technological breakthrough breeding of carp through hypophysation in 1957 freshwater aquaculture of the country the country till 1984 virtually laid the foundation of scientific carp farming in the country. Important characteristics of Indian major carps:- Indian major carp grow fast and can reproduce even in artificial ponds. They feed upon phytoplankton, zooplankton, decaying organic matter, aquatic plant etc. stomach is absent in the alimentary canal of the major carps. Three types of Indian major carps are cultured in

carps form the mainstay of aquaculture in india co259 species of vertebrates recognized worldover 30
The Principal freshwater fishery resources of the Krishna:
Indian major carps: Catfishes:
• C. catla Osteobagrus seenghala
• L. rohita O. aor
• C. mrigala Wallago attu
• Ompok bimaculatus
Other carps: Murrels:
• L. fimbriatus Channa marulius
• L. kontius C. striatus
• P. sarana
• P. dubius
The Principal freshwater fishery resources of the Cauvery:
Major carps: Catfishes:
• C. catla Glyptothorax madrapatanus
• L. rohita Osteobagrus seenghala
• C. mrigala O. aor
• L. calbasu Wallago attu
Minor carps : Silonia silondia
• P. carnatius Other fishes:
• L. kontius Channa marulius
• C. reba Notopterus notopterus
• C. cirrhosa Tor khudree
• P. dubius
v) The West Coast river system
 The West Coast river system includes the basins of the Narmada and the Tapti which supports
varied fauna of commercial importance.
 The other rivers that originate in the Western Ghats possess carps, catfishes, mahseers,
murrels, perches, prawns, etc.
The Principal freshwater fishery resources of the Narmada:
Indian major carps: Catfishes:
Catla catla Rita pavimentata
L. rohita Osteobagrus seenghala
C. mrigala O. aor
L. calbasu Wallago attu
Clupisoma garua
Other carps: O. bimaculatus
L. fimbriatus Mystus cavasius
L. bata
L. gonius Miscellaneous fishes:
C. reba Channa sp
P. sarana Mastocembelus sp
N. notopterus
Tor tor
The Principal freshwater fishery resources of the Tapti:
Indian major carps: Other carps:
L. calbasu L. fimbriatus
C. mrigala P. sarana
Catfishes: L. boggut
Osteobagrus seenghala L. bat
O. aor Tor tor
Wallago attu C. reba
Clupisoma garua
Other fishes:
Channa sp
Mastocembelus sp
1.1.6. Other freshwater resources
Reservoir fish seed resources
 The reservoirs in Uttar Pradesh and Madhya Pradesh, by virtue of their being connected with
the Ganga river system, have a natural stock of major carps.
 But in view of a large volume of water impounded by them, the original stock is being
supplemented by stocking them regularly with major carp fingerlings.
 The reservoirs across other basins, however, do not have a natural stock of major carps.
Hence, major carp fingerlings produced elsewhere were brought and released in them.
Cultivable sp
1.1.7. Coldwater fish seed resources
 The optimum range of temperature for cold water fish is 10-120
C.
 In India, lakes and streams located at more than 900 m above mean sea level qualify for
coldwater. Trouts, salmon and mahseers are the typical cold water fishes in India.
Trout, salmon and char
 Trout and salmon are the only exotic game fishes introduced in India.
The species of trout introduced are rainbow trout, brown trout, eastern brook trout, golden
rainbow and tiger trout (a hybrid between Salmo trutta fario x Salvelinus fontinalis).
 In the Peninsular India trout has been introduced in the Nilgiris and Kodai hills in Tamil Nadu
and in the high ranges of erstwhile Travancore in Kerala.
 In the Himalayas, trout has been introduced in Kashmir and Himachal Pradesh, in Garhwal
Himalayas, Arunachal Pradesh, Nagaland, Meghalaya and in certain waters of Nepal.
Mahseers
 The mahseers are regarded as a sacred fish by the Hindus.
 They are distributed in streams, rivers and lakes in the hilly regions of the Himalayas and the
Peninsular India. They are found in the plains where water is cool, clear with high oxygen
content.
 There are seven species of mahseer belonging to the genus Tor in India. They are: Tor tor, T.
putitora, T. khudree, T. nelli, T. progenius, T. mussullah and T. mosal. Mahseer fishery
resources are protected in temple pools by Temple authorities
Snow trout
 Snow trout (Schizothorax spp.) are believed to have migrated into the lakes and streams of
Kashmir from Central Asiatic watersheds.
 Most of them are now regarded as endemic to the Kashmir valley.
 Presently, there are eleven valid species of Schizothorax. Schizothoracids collected from
different lakes and streams have been successfully induced bred.
Hilsa, Tenualosa ilisha
 The anadromous Indian shad, Tenualosa ilisha (Hamilton), commonly known as hilsa or river
shad is, undoubtedly, one of the most commercially important fish of the country.
 Hilsa ascends the freshwater stretch of all the major river systems from sea mainly for
breeding, thereby forming a lucrative fisheries in freshwater and brackishwater.
 It’s upstream migration has greatly been hampered by the construction of dams, weirs and
barrages across the rivers.
1.2. Marine fish seed resources
This seed resource comprises of brackishwater and marine fish species. India is bestowed with
several species of finfish along its 8100 km coastline.
The most important fish seed resources are
Milkfish: It is distributed in the Indo-Pacific region. Milkfish is found along both the coasts of
India. Primarily milk fish is marine species but it ascends to freshwater zones of river.
Grey-mullets: They are distributed both on the West and East coast of India. They are more
commoly found in the estuarine regions.
Seabass: It occurs in the tropical and sub-tropical areas of Asia in coastal waters, estuaries and
lagoons, including freshwater.

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Pearlspot: Three species of pearlspot inhabit brackishwater and mouths of rivers in Orissa,
Andhra Pradesh, Tamil Nadu, Kerala ands Karnataka.
Sillaginids: They are found in the Indo-Pacific region. In India, they occur in shallow, sandy
bottoms of shores and bays and also in estuaries.
Polynemids: They are distributed in the Indo-Pacific region. They occur along both the coasts of
India. They primarily inhabit shallow muddy and sandy bottoms of the continental shelf,
occasionally entering rivers.
1.3. Natural breeding of finfishes
 Most teleosts are seasonal breeders and their spawning coincides with seasonal changes in
environmental factors.
 In response to environmental stimuli, the hypothalamus secretes a hormone termed as
releasing hormone (RH).
 The Nucleus Lateralis Tuberis (NLT) of the hypothalamus responds to an electrical signal
from the brain by secreting RH at the end of the axon; thus an electric signal becomes a
chemical one (bridging the gap between nervous/neural and hormonal information).
 The arrangement of these neurons/axons is unique in teleosts, their axon directly end on
gonadotropic cells in the adenohypophysis, but do not secrete their hormones into a portal
blood system.
 However, the effect of RH is to stimulate the production of Gonadotropic hormone (GtH) and
its subsequent release into the vascular system of the adenohypophysis.
 GtH is then carried in the general blood circulation to the gonads which in turn secrete
steroids which are required for final maturation and spawning.
The chain of events leading from the reception of environmental stimuli to maturation and release of
gametes is as follows:
1.3.1 Feedback mechanism
 The level of gonadotropins (GtH) is regulated by a process called feedback mechanism.
 It is believed that the release of GtH is adjusted through a system of negative feedback, in
which centers in the pituitary/hypothalamus are responsive to the level of circulating gonadal
steroids.
 A rise in the level of sex steroids brings about a decrease in GtH secretion; with the result the
steroid release again falls to the appropriate level.
 A drop in the steroid level has the opposite effect (a decrease in the steroid level brings about
an increase in the level of GtH).
 In special cases, an antiestrogen or antiandrogen competes with endogenous gonadal steroids
for binding sites in the P-H (pituitary-hypothalamus) axis so that GtH is released regardless of
the level of steroids.
 A negative feedback system becomes then a positive one and results in an artificially elevated
level of gonadal steroids. So both the GtH and gonadal steroids continue to rise and the
pathway becomes positive.
2. Selection of riverine fish spawn collection sites, gear used and methods of collection
 The Central Inland Fisheries Research Institute (CIFRI) located at Barrackpore, Kolkata,
during 1959 – 1964, conducted a pioneering program of seed prospecting investigations on
various river system with a view to ascertaining the quality and quantity of fish seed,
availability, gears for spawn collection, method of collection, measurement of fish seed,
factors responsible for fluctuation in seed availability, etc. on an all-India basis.
 The diverse geographical and climatic conditions of India greatly influence riverine resources
of the country.
 The most important carp seed resources are: eggs, spawn, and fry and fingerlings.
2.1. Egg collection
 Large scale egg collection is possible only where locations of the breeding grounds are known
and are easily accessible.
 Eggs are collected from one or two feet deep water by disturbing the bottom and scooping
them with a ‘gamcha’, a rectangular spawn collecting net.
 Generally, large scale egg collection is not practiced in rivers.
Fry and fingerling collection
 The collection of fry (8-40 mm) and fingerlings (40-150 mm) is usually made by cast and
drag nets.
 Fry and fingerling collection a source of fish seed is prevalent in the Indus river system in
Punjab State.
Fry (14-25mm)
The carp fry can be distinguished from that of catfishes and murrels by the number of dorsal fin rays.
a) Major carps: number of undivided dorsal fin rays >11.
b) Minor carps: number of undivided dorsal fin rays 11 or <11.
c) Catfishes and murrels : Pigmented (either blackish, brownish or orange).
Collection of riverine fish seed using a gamcha, a rectangular mosquito netting cloth
A haul of fish fingerlings from a riverine stretch (Photo courtesy : Dr Utpal Bhowmick)
Fish seed being collected along the bank of a river (Photo courtesy : Dr Utpal Bhowmick)
2.2. Spawn collection
Collection of spawn (up to 8 mm) on a commercial scale is prevalent mainly in Bihar, West Bengal
and Uttar Pradesh
Advantages
1. Traditional and only source of carp seed prior to advent of bundh breeding and hatcheries
2. Collection is cheap and convenient
3. Presently it forms a supplementary source of fish seed
4. Only source for selective breeding programmes
Disadvanges
1. Seed consists of both desirable and undesirable species
2. Undependable source
3. Valuable nursery space is wasted by rearing spawn up to fingerlings
4. Mortality of seed during collection and transportation
2.3. Selection of spawn collection site
Before selecting a suitable site for the collection spawn in a given stretch of river, a pre-monsoon
survey is conducted to ascertain:
1. Topography of the terrain and bank features at the vicinity of the site.
2. Topography of dry bed and bank features.
3. The distribution and composition of fish fauna.
4. The location of tributaries, rivulets and ‘nallahs’ and their confluence with the main river.
5. The identity and accessibility of the site.
A river course showing suitable fish spawns collection sites

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This document discusses paddy-cum-fish culture as a technique for integrated fish and rice farming. It provides details on the history and development of rice-fish culture, site selection considerations, suitable fish and rice varieties, culture techniques, trench designs, advantages and disadvantages. An economic analysis compares the net return from traditional paddy cultivation versus paddy-cum-fish culture, finding that the integrated system provides over double the income. The conclusion states that rice-fish culture is an innovative system that boosts rice yields, generates additional income from fish, and helps reduce farmer poverty while creating jobs.

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1. There are three types of reservoirs in India - small (<1000 hectares), medium (1000-5000 hectares), and large (>5000 hectares). Small reservoirs account for the largest number (19,134) and area (1.48 million hectares). 2. Indian reservoirs tend to be nutrient-rich with narrow temperature fluctuations that prevent thermal stratification in many areas. Biotic communities include phytoplankton, zooplankton, fish, and decomposers like bacteria and fungi. 3. Fish production in Indian reservoirs is low on average at 20 kg/ha compared to potential yields, with room for improvement through management practices like stocking preferred fish species.

fisheries resource management
2.4. Gears used for spawn collection
The most commonly used riverine fish spawn collection net is ‘Shooting net’ which is a funnel shaped
net of finely woven netting.
A typical shooting net (Midnapore type) used to collect riverine fish spawn
A battery of shooting nets ready to be commissioned for riverine/brackishwater fish seed
collection (Photo courtesy: Dr Utpal Bhowmick)
2.5. Indices of spawn quantity and quality
Spawn quantity index: The desirable spawn taken by one standard net during a season is defined as
the index of spawn quantity or yield of a centre.
Spawn quality index: Total percentage of major carp spawn available in a site.
Spawn categories: (i) major carps, (ii) minor carps and (iii) others
2.6. Spawn behaviour in relation to hydrological and hydrobiological characters
Flood level: Flood is the most important character showing a positive correlation with spawn
availability.
Current velocity and water depth: Velocity ranging between 0.5-3 km per hour is conducive to
spawn collection.
Current direction: A constant direction of current is ideal for spawn collection
Weather: Overcast sky, with or without drizzle, coupled with gentle breeze, is generally ideal for
spawn collection. Windy weather is unfavourable. Night collections are higher than day collections.
Unit 2 - Sexual maturity, breeding season and development of gametes
2.1. Sexual maturity
 Maturation of fish gonads (gonadal maturation) is a process by which minute sex cells (germ
cells) develop, with accessory tissues, into large organs (testis and ovary) which finally lead
to the maturation and release of gametes (sperm and eggs).
 Maturation may occur once in the life spawn of a fish or once a year or many times a year,
depending on species and conditions.
 Age at sexual maturity in fish varies with climatic and ecological /environmental conditions.
It varies between species and between sexes. In general, males mature earlier than females.
 Size at first sexual maturity also varies with species and between sexes.
2.2. Breeding season
 The cultivated carps (with the exception of common carp) attain maturity at the end of second
year or the beginning of third year, depending upon the temperature.
 They mature during May-June and some are found to remain mature till the end of August.
 Majority of the major carps breed during south-west monsoon, in flooded rivers.
 The common carp attains maturity at 4-6 months of age and breed year-round in captivity.
 Like the common carp, tilapia matures at 4-5 months and spawns through out the year.
 Medium carps, catfishes, murrels, etc. mature during pre-monsoon and with the onset of
South-West monsoon and breed in ponds, tanks, paddy fields, etc.
 Brackish-water and marine fishes attain maturity during pre-monsoon and with the onset of
South-West monsoon and have prolonged breeding season.
 Gonadotropins regulate the formation and maturation of gametes indirectly through steroid
hormones produced by gonad itself.
 Maturation has various stages and different sex steroids are involved at each stage, but
gonadotropins influence the production of all of them.
2.3. Gamete maturation and release in females
Gametes develop and mature in the female fish through a series of stages, before being released. The
stages are given below
1. Oogenesis
 Oogonia are the cells that give rise to oocytes through a continuopus process called oogenesis
and are found throughout the life of a fish.
 Oocytes are produced from oogonia as a result of meiosis (reduction division).
 Afer the first meiosis the oocytes become surrounded by a layer of epithelial cells called the
follicle.
 After this the oocyte enters a long stage of cytoplasmic growth.
 In fish that breed more than once, oogenesis goes on at various rates throughout the fishes life
and is not under the control of hormones (GtH).
2. Primary oocytes growth
 The early development of the follicle and its oocyte is independent of pituitary GtH.
 The growth is due mainly to proliferation of cellular components.
 By the end of this stage, the typical teleost oocyte increases several hundred times in size to a
diameter of 100-200µm and is called pre-vitellogenic oocyte.
 During the growth period, the follicle cells differentiate to form glandular granulose,
separated from oocyte by a zona pellucida and surrounded by an outer theca.
 These cells play an important role in steroidogenesis.
 The primary growth process continues throughout the life of fish that are multiple spawners
and previtellogenic oocytes are present in the ovary year round.
 If only the previtellogenic oocytes are present, the ovary is considered immature, which may
be became of age or season.
3. Yolk vesicle formation
 In response to environmental cues- change in day length, temperature or rainfall, there will be
a surge in the GtH levels that induce previtellogenic oocyte to develop further.
 The first sign of this stage is the appearance of yolk vesicles in the oocyte cytoplasm.
 There contains glycoproteins formed within the oocyte and will eventually become cortical
alveoli (may be a source of energy for the embryo) that will be expelled into the previtlline
space around the egg after fertilization.
 This process is some times referred to as “endogenous vitellogenesis.”
 Since there oocytes contain neither true yolk nor vitellogenin, it is better known as “yolk
vesicle formation” which is triggered by GtH.
4. Vitellogenesis
 Sequestration of the phospholipids, vitellogenin (Vg) from the blood stream and accumulation
of true yolk in yolk globules takes place after yolk vesicle formation. This represents the
major growth of the oocytes and is known as vitellogenesis.
 It involves synthesis of Vg (yolk protein precursor) in the liver, its dlivery to the oocytes via
bloodstream and uptake and chemical alterations to form yolk protein.
 GtH induces the thecal cells of the follicle to produce testosterone (T), which in turn is
converted to an estrogen, 17β -estradiol (17βE2), in the granulosa cells.
 E2 travells to the liver in the blood and stimulates production of Vg, which travels to the
oocyte, also by the blood stream.
 Oocytes sequester Vg as yolk protein in yolk globules and increase in size and this is
facilitated by GtH.
 The plasma levels of GtH is high during this phase of maturation. T and E2 act on the
pituitary in “feed back loops” to regulate GtH release.
 Vitellogenesis can be triggered and accelerated by environmental manipulation and hormonal
manipulation.
5. Steroid switch and final maturation
 Final oocyte maturation in many warm water species is rapid- it usually takes less than 24hrs.
 It involves resumption of meiosis, migration of GV (Germinal vesicle) to the edge of the
oocyte and GVBD (Germinal vesicle break down), an event that is useful in inducing the
oocyte maturity.
 Meiosis then stops again and the oocyte is now mature, ready for expulsion from the follicle
(ovulation).
 During this stage, the level of GtH increases and GtH stimulates the follicle to produce
maturation inducing steroid (MIS) instead of estradiol.
 MIS is a form of progesterone called 17α - hydroxy progesterone (17α-OHP) or 17α, 20 β-
dihydroxy progesterone (7α-20β-diOHP).
 At this stage the enzyme responsible for the production of E2 is inhibited. How this steroid
switch is regulated is not known.
 The MIS induces a number of visible changes in the oocyte during final maturation.
 In addition to GV migration and breakdown, it causes an increase in oocyte diameter due to
the uptake of water (hydration) in to the cytoplasm and changes in the appearance of the yolk.
6. Ovulation
 Final maturation is followed by ovulation- the release of egg from its follicle into the ovarian
lumen and is ready to be expelled into the surrounding water for fertilization; ovulation is
controlled by prostaglandin (PG) under the influence of GtH.
 PG is produced by the follicle and the oviduct; PG are cyclopentane fatty acids.
 Once ovulated the eggs of different species remain fertile within the ovary or body cavity for
periods of <1 hr to several days.
 After this time the eggs become “overripe” and start to disintegrate.
 In fish, eggs remain fertile only briefly, (few hours), eg. Carps.
 Eggs remain fertile for days, eg. Salmonids
 Over-ripening of eggs held in the body after ovulation are highly temperature dependent.
 In hormone induced spawning, final maturation and spawning are the most important stages
of reproduction.
Mature female with orange/yellow ovary
2.4. Gamete maturation and release in males
 Fish endocrinologist or breeders are normally biased towards events in the female such as
vitellogenesis, final maturation and ovulatory (the three important stages of reproduction).
This is because gonadal development in the male goes ahead on its own even in captivity.
 In nature, male remains mature over a much longer period than the females and spermatozoa
can remain fertile in the testis or outside for much longer periods (up to several days)
compared to ovulated eggs which loose fertility very quickly.
 However, milt may be scarce and of poor quality, particularly in fishes which need to be
induced bred (carps and milk fish) an understanding of sperm formation will be useful in
overcoming these practical difficulties.
 The testis of fish is sac-like and folded.
Like the oocyte, male gametes (sperm cells) develop and mature in the male fish through a series of
stages. The stages are given below

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1. Spermatogenesis
 Spermatogonia are cells that give rise to spermatocytes through a process called
Spermatogenesis.
 The spermatocytes produced by meiosis (I) are enveloped by sertoli cells until their liberation
as mature spermatozoa with in the lumen of the testis.
 GtH induces the Leydig cells to produce Testosterone (T), which causes spermatogonia to
divide into spermatocytes within cysts of sertoli cells.
2. Spermiogenesis
 Development of spermatocytes into spermatids (spermiogenesis) may continue and occurs
through 2nd meiosis in the absence of GtH.
 The growth of spermatids takes place within the cyst of sertoli cells and at this stage the
spermatids possess flagellum.
3. Spermiation
 Mature spermatids are liberated into the lumen as spermatozoa through a process called
spermiation.
 11-ketotestosterone (11-KT), produced by the Leydig cells under GtH stimulation, initiates
this process.17α-20β-DiOH P, also produced by the Leydig cells (and possibly by mature
spermatozoa), maintains continuous low level spermiation.
4. Hydration
 The last step before release of spermatozoa is hydration i. e. dilution of spermatozoa with
seminal fluid produced from the walls of the sperm duct under the influence of 17α-20β-
DiOH P.
 This diluted sperm suspension is called milt which is released during spawning.
 Males are considered ‘ripe’ once spermiation starts, although low level spermiation can go on
for several months in some species.
 Spermiation and sperm hydration increase rapidly when the appropriate environmental cues
are present and are often synchronized with final maturation in females.
 Males generally are mature even in captivity, if not, environmental/hormonal manipulation
can induce spermiation.
 Hence males need less hormone than females to induce ‘final maturation’.
Mature male with creamy white testis
Unit 3 - Breeding of major carps
3.1. Bundhs
 Bundhs are special type of tanks or impoundments where riverine conditions are simulated
during monsoon months for breeding carps.
 They may be perennial (wet bundhs) or seasonal (dry bundhs).
 Bundh breeding, which accounted for 5.4% in 1964-65, contributed to about 63% in 1980.
 Bundh breeding is popular in Madhya Pradesh and West Bengal.
 Bundh breeding seems to have its origin in West Bengal.
 Majority of bundh-type of tanks, where major carps are known to breed, are located in the
districts of Midnapore and Bankura in West Bengal and around Nowgong in the Chhattarpur
district of Madhya Pradesh.
 The first dry bundh was located in Sonar Talliya in Nowgong district of Madhya Pradesh.
 The initial success achieved by the Department of Fisheries, Government of Madhya Pradesh,
during 1958 in dry bundh breeding of carps in Sonar Talliya led to the construction of many
more dry bundhs with improved designs.
 The most modern constructions are generally masonry structures with arrangements for a
sluice gate in the deepest portion of bundh for complete draining and one or two waste weirs
for overflow of excess water.
 In most cases, apart from the bundh itself, a dry bundh unit consists of storage ponds for
stocking breeders, an observation tower with arrangements for storing necessary equipment
and a set of cemented hatcheries (2.4m x 1.2m x 0.3m) with a regular supply of water for a
large number of eggs at a short time.
 In some cases, the embankment is a pucca stone masonry with a small sluice gate and a
portion of the embankment itself serves as the waste weir (Dubey, 1969).
3.1.1. Wet bundh
A typical wet bundh of Midnapore (West Bengal) is a perennial pond or tank, situated in the slope of
a vast catchment area of undulating terrain, with proper embankments having an inlet towards the
upland and an outlet towards the opposite lower end.
 During summer, only the deeper portion of the bundh retains water where carp breeders are
released for spawning.
 The remaining portion is dry and is many times used for agricultural purposes.
 After a heavy shower, a major portion of the bundh gets submerged with water from
catchment area (catchment area: bundh, 20-100: 1), coming into it in the form of streamlets.
 The depth of water where breeding takes place has been stated to vary from 8 cm to 1.2 m.
 Though the fish breeds at any spot in the bundh, it may be advantageous to prepare `spawning
grounds’ at different levels which could get flooded at different water levels in bundhs.
 The bundhs can also be filled with water as and when desired and breeding operations
undertaken without waiting for the fresh rainwater.
Method of breeding carps in a wet bundh
 With the onset of monsoon the rain water from the catchment area gushes into the bundh,
creating an artificial current.
 The breeders, already present in the deeper area of the bundh, migrate to the shallower areas
and start breeding.
 After the breeding is over, egg/spawn collection is done.
3.1.2. Dry bundh
 A typical dry bundh is a shallow, seasonal depression, having a bundh on one side and a
catchment area on the other three sides.
 The bundh may be of varying shape and size and made of earthen wall or masonry wall.
 A dry bundh is smaller and shallower than a wet bundh which is bigger and deeper.
 The bundh gets flooded in monsoon, but remains completely dry for a considerable period
during a year.
 It consists of a sluice gate for quickly draining the water and an outlet for the excess water to
flow away. In dry bundhs, spawning sometimes takes place in deeper areas.
Technique of breeding major carps in a dry bundh
 The mature carp breeders which are raised in perennial ponds elsewhere are introduced into
the bundh at 1:2 (Female: Male).
 The fish are left undisturbed for 2-3 days so that they get acclimatized to new environment.
 After this, 10-20% of the fish is given intramuscular injection of pituitary extract or ready–to-
use spawning agents which are synthetic hormones.
 Water current is created in the bundh by drawing water from a store tank.
 The following morning, the spent breeders are removed, eggs collected, water drained and the
bundh dried for 2-3 days.
 The bundh is then utilized for the next breeding by releasing a fresh batch of breeders.
 Five to six spawning are generally conducted in each bundh during one season as opposed to
only one spawning in a wet bundh.
 Silver carp and grass carp have been successfully induced bred in bundhs without stripping.
 Sinha et al. (1979) have reported natural spawning of both grass carp and silver carp in a dry
bundh of Bankura District where they were able to spawn the two species without stripping.
 They consider dry bundhs to be one of the reliable means of mass breeding of Chinese carps
to meet the increasing demand of their seed.
3.1.3. Collection and hatching of eggs
 After spawning is over, the eggs are collected from bundhs, after lowering the water level, by
dragging a piece of mosquito netting cloth (gamcha) and released for hatching either in
improvised pits or double-walled hatching hapas or cement hatcheries.
 The hatching pits (448 cm x244cm x 46 cm) are excavated on the bank with arrangement for
the supply of water.
 Each pit may contain about 0.9 to 2.2 million eggs, of which 2.5-25% hatch successfully.
 A double-walled hapa, which is fixed in the bundh itself, consists of an outer hapa (182 cm
x91 cm x 91 cm) and an inner hapa (152 cm x 76 cm x 46 cm ), accounts for a spawn survival
rate of 32 to 50%.
 The provision of cement hatcheries (2.4 m x 1.2 m x 0.3 m) near the dry bundhs in Madhya
Pradesh has aided in improving the survival of hatchlings to 97%.
 A cement hatchery of Madhya Pradesh has three times more capacity than a double-walled
hapa and is far more economical than the latter. Since the collection of all the eggs is
impossible, especially in the case of wet bundhs in view of their larger size, fry and fingerling
collection is equally important.
3.1.4. Factors responsible for breeding of fish in bundhs
 Spawning may occur at night and during bright sun in the forenoon.
 After a period of breeding behaviour, mating occurs with vigorous splashing of water and a
number of scales may get dislodged while some fish may even sustain minor injuries.
 After spawning is over, a thick blanket of eggs is left behind at the spawning site.
 The spent fish in bundhs move to the deeper areas.
 No single factors can probably be attributed to spawning of major carps in bundhs and rivers.
 The act of spawning involves the completion of a chain of interrelated pre-conditions.
1. Heavy monsoon flood capable of inundating vast shallow areas is believed to be a
primary factor responsible for spawning. Some workers believe the availability of
shallow spawning ground to be a deciding factor for spawning. The rise in the level
of water, naturally or artificially, is known to bring about spawning.
2. The temperature of water for spawning is found to be between 22 and 33°C.
3. Other factors like pH, high Dissolved Oxygen, alkalinity, chloride and minerals do
not seem to play any significant role in spawning.
Soil type is not very important.
4. Spawning is inhibited due to the presence of hormone-like secretion in captive
waters.
5. Water that has flown through a dry bed of land rich in humus has stimulatory effect
on spawning.
3.2. Induced breeding of warmwater finfishes and environmental factors affecting spawning
 Houssay (1930) of Argentina was the first to attempt induced breeding of fish by using
pituitary extract on a viviparous fish. He was successful in obtaining premature birth of young
fish.
 Subsequently, based on the lines of Houssay, Von Ihering and his team of Brazil, in 1934,
successfully induced bred a catfish with pituitary hormones and hence credit for the present
day concept of induced breeding of fish goes to Brazilians.
 In India, Chaudhuri and Alikunhi (1957) successfully induced major carps to spawn through
hypophysation technique.
 Since then, the technique has been standardized and refined for the large-scale production of
fish seed.

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Asian sea bass culture
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Asian sea bass, also known as giant perch or Bhetki, is a profitable species for aquaculture in Southeast Asia. It can be cultured in both freshwater and saltwater. Hatcheries in Thailand produce sea bass fry that are also exported to other countries. Major challenges for sea bass culture are cannibalism among young fish and dependence on fishmeal. The life cycle involves nursery rearing of fry from 2-5 grams to 5-10 centimeters, then grow out in ponds or cages to a market size of 300-400 grams in 3-4 months or 700-1200 grams in 8-12 months. Sea bass is commonly cultured with tilapia in polyculture systems.

 The Indian Major Carp, which normally spawn once a year either naturally or through
hypophysation during monsoon, were successfully induced bred twice within an internal of
about two months.
 Chondar (1984;1990) described a method for the mass scale breeding of IMC and silver carp
in `Bangla bundh’ through Human Chorionic Gonadotropin (HCG) and its combination with
pituitary extract.
A major carp being injected with a spawning agent for induced breeding
3.2.1. Environmental factors concerned with breeding of fishes
Environmental factors concerned with fish breeding are
 Light
 Temperature
 Ecological factors
 Meteorological conditions
These factors are known to play important roles in stimulating the release of pituitary gonadotropins,
thereby controlling reproduction in fish.
Light
 It is an important factor that controls reproduction in fish.
 Early maturation and spawning of fish as a result of enhanced photoperiodic regimes.
 In India, Cirrhinus reba was found to attain early maturity when subjected to artificial day
lengths longer than natural day even at a low temperature of the winter months, viz. 19-20o
C.
 The resorption of gonads in C. reba was delayed and spawning conditions could be
maintained up to November.
Temperature
 The role of environmental temperature on sexual maturation and spawning of fish in India has
been studied.
 All observations show that there are optimum temperature ranges for induced breeding of
cultivable fishes and critical temperature limits, above and below which fish will not
reproduce.
 The Indian Major Carps are found to breed within a range of 24-31o
C.
Beyond this range fish do not spawn.
 The Chinese silver and grass carp have been successfully induced bred at temperatures 28.2o
C
to 34o
C.
 It was observed natural spawning of pituitary injected grass carp at a water temperature
varying between 28.9 and 31.1o
C, the optimum being 27o
C, as in the case of Indian Major
Carps.
Other Factors
 It was opined that fresh rainwater and flooded condition in a tank are the primary factors in
triggering the spawning of carps.
 The presence of repressive factors may be responsible for inhibiting spawning of carps in
confined waters, but when this repressive factor is sufficiently diluted by the onrush of floods
in bundhs or ponds, spawning occurs.
 Some workers suggested that it is the sudden drop in the electrolytes level in the environment
caused by heavy monsoon rain or water current which induces gonadal hydration, resulting in
natural spawning of carps.
 Rain water and weather condition are important factors for induced breeding of fish.
 Successful spawning in the majority of fishes has been induced on cloudy and rainy days,
especially after heavy showers.
 The carps are known to breed at a fairly wide range of pH and dissolved oxygen content.
3.2.2. Sympathetic breeding
 Sympathetic breeding refers to the breeding of uninjected fish at the sympathy of injected
fish.
 This is common in bundh breeding, wherein, only 10-20 brooders are injected with either
pituitary extract or synthetic spawning agent and the rest are not injected.
 After an interval of about 8-10 hours, the injected brooders first start spawning and
subsequently the uninjected brooders are also stimulated to spawn, thereby leading to the
complete spawning of all the brooders.
 Sympathetic spawning leads to lesser use of hormone and reduced handling of brooders.
 By this method, natural spawning of both grass carp and silver carp is possible in a dry bundh
of Bankura District where they spawned naturally, without stripping.
 Some consider sympathetic breeding as one of the reliable means of mass breeding of
Chinese carps to meet the increasing demand of their seed.
3.3. Fish Pituitary gland
 Pituitary gland is an endocrine (ductless) gland situated on the ventral side of the brain.
 It is a small, soft, whitish body whose size and shape vary with species.
 It is more or less round in carps; oval in catla and rohu and pear-shaped in mrigal.
 The pituitary is located in a concave cavity known as Sella turcica and enclosed by a thin
membrane known as duramater.
 It may be attached to the brain by a short stalk called the Infundibular stalk.
Types of pituitary glands
Based on the presence or absence of the stalk, the pituitary is classified into
 Leptobasic pituitary (with stalk)– eg. Carps and catfishes
 Platybasic pituitary (without stalk)– eg. Murrels and glassfish (Ambasis species)
The teleost pituitary comprises of two parts-
 The glandular part (the adenohypophysis)
 The nervous part (the neurohypophysis)
3.3.1. Collection of pituitary gland
Fish pituitary gland can be collected by dissecting and removing a portion of the scalp or through the
Foramen magnum.
(1) Dissecting and removing a portion of the scalp
 In this method, the brain case (cranium) is obliquely cut using a butcher’s knife/hand
saw/bone cutter and the scalp removed.
 The brain is then exposed by removing grey matter and fatty substance with forceps and
cotton.
 The anterior end (optic and olfactory nerves) of the brain is cut and the entire brain is lifted up
and laid back, thus exposing the pituitary under a membrane.
 After removing the membrane and the fluid, the pituitary is lifted up by inserting the blunt
end of the forceps and carefully transferred to a vial containing a preservative.
Making an oblique cut in the cranium Fatty tissue and grey matter exposed
The brain being exposed the pituitary seen as a small whitish body
The pituitary mounted on to a wrist
(2) Through the Foramen magnum
 Foramen magnum is a large posterior aperture of the skull through which the spinal cord
passes.
 The grey matter and fatty substance are first removed with the help of forceps and cotton
(they are pulled out posteriorly).
 The brain is then exposed.
 After this, the anterior end (optic and olfactory nerves) of the brain is cut and the entire brain
is lifted up and laid back, thus exposing the pituitary.
 After removing the fluid the membrane, the pituitary is lifted up by inserting the blunt end of
the forceps and carefully transferred to a vial containing a preservative.
The first method is commonly practiced even though the second method is less time consuming and a
large number of glands can be collected within a short time, with a good resale value of the fish.
3.3.2. Preservation of pituitary gland
1. Preservation in absolute alcohol
 In this method, the gland, after collection, is immediately transferred to a vial/phial containing
fresh absolute alcohol (ethanol).
 After 24 hours, the alcohol is removed and fresh alcohol is added and stored at room
temperature or in a refrigerator.
2. Preservation in acetone
 Immediately after collection, the pituitary gland is kept in ice-chilled acetone and stored in a
refrigerator for 2-3 days.
 After this period, the acetone is changed and the gland stored in a refrigerator.
 Both absolute alcohol and acetone have de-fattening and dehydrating effect.
3. Immediate freezing
 In this method, the collected glands are frozen immediately and stored in a freezer.
3.3.3. Preparation of fish pituitary extract for injection
 The extract preparation should be carried out just before injection.
 The required quantity of glands is taken out of vial and they are dried on a filter paper by
allowing the alcohol to evaporate.

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 The glands are then homogenized with distilled water or saline in a tissue homogenizer.
 If acetone-dried glands are used, they can directly be taken for maceration.
 One-third of the media is used for homogenization, while the remaining two-third is used for
rinsing the homogenizer and the glass rod.
 Recommended dilution rate is 20-30 mg in 1 ml of the media.
 The extract is centrifuged at 5,000 rpm for 5 minutes.
 The clear supernatant solution containing gonadotropins is taken in syringe for injection.
Types of injection
Homoplastic injection: Injecting pituitary from one fish to another fish closely related to the donor
fish. E.g. carp pituitary gland extract to carps.
Heteroplastic injection: Injecting pituitary from one fish to another fish distantly related to the donor
fish. E.g. carp pituitary gland extract to catfish and vice versa.
Methods of injecting fish brooders
There are three methods of injecting brooders.
They are:
1. Intra-muscular injection:
 It is administered into the muscle on the caudal peduncle or behind the dorsal fin, but above
the lateral line.
 It is most effective, convenient, simple and less risky.
 It is widely practiced.
2. Intra-peritoneal injection:
 It is give through the soft regions of the body, generally at the base of the pelvic fin or the
pectoral fin.
 It is risky as it may damage the gonads or liver.
3. Intra-cranial injection:
 In this method, the injection is given through the cranium and is also risky as it may damage
the brain.
 The pituitary extract is administered through a glass or disposable syringe, 2.0 ml capacity,
having 0.1 ml graduation.
 The size of the needle depends upon the weight of the brooder to be injected.
 Needle number 22 is used for fish weighing 1-3 kg, No. 19 for larger fish and No. 24 for
smaller fish.
 When two injections are given, one is given on the side that did not receive the first injection.
3.3.4. Dosage of pituitary extract
 Assessment of proper dosage is most important for successful spawning. In practice, the
female receives two injections, while the male receives only one injection, i.e. at the time of
second injection to the female.
 I Dose or Provocative or preliminary dosage and II Dose or effective or resolving dosage.
 The interval between the two doses is 6 hours.
 Carp glands to major carps
Female Male
I Dose 2-3 mg/kg b.w. nil
II Dose 5-8 mg/kg b.w. 2-3 mg/kg b.w.
 Carp glands to exotic carps
Female Male
I Dose 4-6 mg/kg b.w. nil
II Dose 10-16 mg/kg b.w. 4-6 mg/kg b.w.
 Catfish glands to major carps
Female Male
I Dose 10 mg/kg b.w. nil
II Dose 20 mg/kg b.w. 10 mg/kg b.w.
 Catfish glands to exotic carps
Female Male
I Dose 20 mg/kg b.w. nil
II Dose 40 mg/kg b.w. 20 mg/kg b.w.
3.4. Synthetic hormones for induced breeding of fishes
 Studies conducted by numerous investigators on induced breeding of fishes have indicated the
superiority of several ovulating agents over fish pituitary extract.
 Although fish pituitary extract was initially used extensively for fish breeding all over the
world, synthetic spawning hormones are now being increasingly used due to their efficacy
and convenience.
 Banerjee et al. (1989) succeeded in the purification of pituitary gonadotropic hormone from
Channa punctatus and Catla catla.
 Mammalian pituitary hormones in combination with fish pituitary gland extract precipitated
spawning in fish.
 Of all the mammalian hormones tested on fish, chorionic gonadotropin (CG) has given
successful result in inducing fish to breed, probably because CG behaves primarily as a
luteinising hormones (LH).
 Synahorin (a mixture of CG and mammalian pituitary extract) in combination with pituitary
gave positive results when injected to rohu.
 Sinha (1969) reported the fractionisation of pituitary extract from carps and tilapia. He
obtained success in spawning of carps.
 Bhowmick et al. (1979) found mammalian hormones antuitrin-s, leutocyclin and RH-LH
ineffective when injected singly or in combination with carp pituitary extract.
 The CIFRI, Barrackpore undertook detailed studies on the use of LH-RH alone or in
combination with progesterone and obtained breeding success which ranged between 25-49%
in carps and 100% in catfish.
3.4.1. Synthetic spawning agents
 The stimulation of pituitary gonadotropin secretion by synthetic LH-RH has been
demonstrated in a number of teleosts.
 Since LH-RH (natural or synthetic) alone is not very effective in inducing spawning in fish, a
combination of LH-RH-a (GnRH-a) and a dopamine antagonist for induced ovulation and
spawning in cultured fish is a highly effective procedure called the Linpe method.
 Some workers reported successful spawning of catla, rohu and mrigal with LH-RH analogue
at 10-20 mg/kg b.w. and also obtained 100% ovulation with pimozide at 10mg/kg b.w.
 Parameswaran et al. (1988) achieved successful spawning in mrigal with LH-RH-a, buserelin
acetate in combination with progesterone.
 Investigations of Jose et al. (1989) with LH-RH-a indicated successful breeding of mrigal and
Labeo fimbriatus.
The Linpe method and ovaprim
 Both of these rapidly gained acceptance in fish farms in China and India and has now been
commercialized by Syndel Laboratories, Inc., Vancouver, British Columbia, Canada, under
the tradename ovaprim.
 The ovaprim spawning kit is especially formulated for use with salmonids, cyprinids and
other freshwater cultured fish.
 It has been used successfully in a number of species in several countries and is gaining wide
acceptance as the preferred method for induced ovulation and spawning of cultured
freshwater fish.
 For example, in India, based on field trials (during 1988-90) with ovaprim for induced
spawning of Indian major carps, fringe lipped carp, silver carp, bighead carp and grass carp in
various fish farms located in different agro-climatic regions, Nandeesha et al. (1990, 1991)
concluded that in economic terms, the use of ovaprim is advantageous.
 The spawning success, quantity of eggs obtained, the fertilization rate and hatching
percentage remained consistently higher with ovaprim as compared to carp pituitary extract
(CPE) or human chorionic gonadotropin (HCG) in almost all instances.
 The results also indicate that nearly 40% more fry can be obtained by using ovaprim in place
of commercial CPE.
 Most of the carps tested generally spawned within 10-14 hours after injection. Ovulation and
spawning has been successfully induced in India by the Linpe method in the Asian catfish,
Clarias batrachus (Manickam and Joy, !989) and Indian catfish, Heteropneustes fossilis
(Manickam, 1992).
 Similarly, indigenous preparations, viz. Ovatide (M/s. Hemmopharma Ltd., Mumbai) and
WOVA-FH (M/s. WOCKHARDT Ltd., Mumbai) are also being used commonly for the
commercial spawning of carps and other fishes in India.
 A combination of busereline (LHRH-a) and domperidone has been successfully used for the
spawning of IMC (Basavaraja et al., 2007).
3.4.2. Dosage of ready-to-inject spawning agents (ovaprim, ovatide, WOVA-FH, etc.)
Females
 Catla : 0.4-0.5 ml/kg b.w.
 Silver carp : 0.4-0.7 ml/kg b.w.
 Rohu : 0.3-0.4 ml/kg b.w.
 Grass carp : 0.4-0.8 ml/kg b.w.
 Mrigal : 0.25-0.3 ml/kg b.w.
 Bighead carp : 0.4-0.5 ml/kg b.w.
 Fringe-lipped carp : 0.3-0.4 ml/kg b.w.
 Mahseers : 0.6-0.7 ml/kg b.w.
 Catfishes : 0.6-0.8 ml/kg b.w.
Males (all species of carps) : 0.1-0.3 ml/kg b.w.
Males (catfishes) : 0.15-0.4 ml/kg b.w.
Steroids
 An alternative approach is to use selected steroid hormones targeted at the oocytes. Most of
the previous work on this subject is on induced ovulation in vitro.
 The effects of steroid hormones on ovulation are seen primarily as germinal vesicle
breakdown (GVBD).
 GVBD is normally controlled by one or more steroids produced in the ovaries under
gonadotropin stimulation, but the timing of ovulation related to that of GVBD varies.
 In vitro ovulation normally follows steroid induced GVBD in catfish (H. fossilis). The action
of pituitary gonadotropins on final oocyte maturation is known to be medicated through
steroid hormones.
 Deoxy corticosterone acetate (DOCA) and cortisone effectively stimulated in vitro ovulation
in H. fossils.
 The available reports indicate that steroid hormones are quite potent in inducing spawning in
cultivated fishes, but are yet to find commercial applications.
 The thyroid stimulating hormone is also reported to bring about ovulation in Indian catfish.
 Although there are no reports on the effects of pheromones on the reproduction of IMC, there
are circumstantial evidences which suggest that pheromones secreted by IMC help in
effecting spawning.
 Similarly, sympathetic spawning of carps in bundhs appears to be due to the release of
pheromones.
3.5. Fish broodstock management and transportation of broodfish
Management of broodfish ponds
 Brood fish is a prerequisite for all induced breeding programmes, as it produces eggs and
milt, which are required for the production of larvae.
 Proper brood-stock will lead to better breeding responses, increased fecundity, fertilization,
hatching and larval survival rates and more viable fish seed.

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 Hence, the subject of brood fish management has assumed great importance in hatchery
management.
 The number of brood fish ponds depends on hatchery requirements.
 Large-scale of operation and sex-wise segregation of fish requires more ponds.
Carp broodfish pond
 Carp brood-stock ponds are generally large (0.2-2.5 ha), 1.5-2.5m deep, 30-40m wide,
rectangular, seasonal or drainable and earthen in nature.
 Water inlet and outlet should be such that they simulate riverine/fluviatile conditions, which
is the natural habitat of IMC and Chinese carps.
Source of broodfish
Since selective breeding and hybridization programmes of pedigreed fish are not carried out in fish
seed farms, the source of future broodfish is stock ponds from the same farm or different farms or live
adult of different species procured from capture fishery waters like rivers, lakes or reservoirs.
Care of broodfish
 The carp brood-stock pond should be prepared following standard procedure to ensure
sustained production of zooplankton.
 The recommended stocking density of carp brood fish is 1,250-2,500 kg/ha, depending upon
the species.
 While rohu and mrigal are stocked at a higher rate, catla is stocked at a lower rate since it
requires more space for proper gonadal development.
 Stocking rates are manipulated to permit individual and collective care of broodfish, enabling
them to get nutritional and environmental advantages for onset of right degree of maturity.
 During immature stage, feed the fish with a traditional diet consisting of rice bran and oil cake
(1:1) at a feeding rate of1- 2% of body weight daily.
 During the maturing phase, feed the fish with a special feed containing rice bran, oil cake, fish
meal, cereals, grams and mineral and vitamin mixture.
 Alternatively, one can use commercial floating pelleted feed (protein content : 30%)
 In addition to the artificial feed the grass carp is also given tender aquatic weeds/terrestrial
grass.
 However, the breeding habits of some species like common carp demand their separation
from other carp species due to their natural breeding in ponds with aquatic vegetation.
 As a result the common carp brood fish is segregated sex-wise and stocked in separate ponds
to prevent accidental spawning in pond.
 However, the rest of the species can be stocked in a communal pond or stocked in separate
ponds after species-wise and/or sex-wise segregation.
 Catla, in particular, needs to be separated from the rest of the species as it shows poor
response to hormonal injection when stocked with other species.
 A gravid fish when held by hand with tail up should practically ooze milt and also ova.
 Paddle-wheel aerator, particularly in catla pond, can provide additional aeration, particularly
during morning hours.
 Segregation of sexes at least one month before increases the affinity between male and female
during spawning.
 Care should be taken to maintain water quality and plankton level by periodic manuring, i.e.
at one tenth of the initial dose.
 Algal blooms and oxygen depletion are controlled by water exchange.
 Parasites and pathogens should be controlled by periodic checking of brooders
 Common parasites like Lernea and Argulus are common on major carps (catla is more
susceptible) can be controlled by manually removing and disinfecting the affected fish with a
solution of KMnO4 (about 5 ppm)
3.5.1. Broodstock management practice
Proper brood fish management forms the key to successful spawning. The number and quality of eggs
produced are significantly affected by the conditions under which the brood-stock is maintained.
 The quality of brood-stock diet, feeding regime, the quality of brood-stock and water
management are the principal factors that influence the condition of the broodstock.
 Most seed farms raise broodstock in their own farm (there are instances of inbreeding
depression, as reported by Eknath and Doyle (1985) and maintain them in ponds at a density
of 1,000-2,500 kg/ha.
 The earthen brood-stock ponds vary in area from 0.2 to 1.0 ha, with depth ranging from 1 to 2
m.
 The farms use water from perennial reservoirs.
 The number of brood fish ponds varies with hatchery requirement.
 The main basic steps in the preparation of broodstock ponds are : control of aquatic weeds,
which in done manually; eradication of unwanted fish by applying mahua oilcake at 2,000-
2,500 kg/ha and pond liming at 100-200 kg/ha depending on the pH of soil and water.
 This is followed by fertilizing the pond with cattle dung, at 15,000-20,000 kg/ha/yr or poultry
manure at 5,000-10,000 kg/ha/yr to enhance heterotrophic food production.
 In addition, 200-400 kg/ha/yr NPK mixture is applied in split doses at fortnightly or monthly
intervals.
 The initial dose of organic manure is reduced by half if mahua oil cake is used as piscicide.
 After stocking the pond with carps that are one-year-old or more, they are fed with a
conventional feed containing a mixture of groundnut oil cake and rice bran (1:1 or 1:2 ratio)
at 1-2% b. w., once daily.
 To ensure better and timely development of gonads, fish breeders use a special broodstock
diet (protein : 25-30%) prepared using locally available cheap ingredients.
 This diet is nutritionally superior, advances maturation and spawning by one or two months
and results in increased fecundity and better seed quality.
Ingredients %
Rice bran 25
Groundnut oil cake 25
Fish meal 10
Maize 10
Broken rice 10
Horse gram 10
Blackgram 10
 This diet is given at 2% b.w. daily, starting in December.
 At some farms about a third of the broodstock will be injected with a low dose of HCG at 6-7
mg/kg body weight every 20 days, starting from mid-February for advancing maturation so as
to induce spawning by the end of May.
 At some seed farms, a few vitamin E tablets are mixed, in addition to a small quantity of
commercially available vitamin and mineral mix, in the diet to facilitate gonad development.
 Algal blooms and oxygen depletion are the most common problems encountered in
broodstock ponds. These can be overcome by frequent water exchange.
 Although no major diseases occur in these ponds, infestation by Lernaea and Argulus on catla
are common and are controlled by manual removal of adult specimens, followed by a dip
treatment in a mild solution of potassium permanganate.
3.5.2. Transportation of broodfish
 It is not economically feasible to transport bigger fingerlings/yearlings and broodfish in small
packing containers.
 For this purpose, truck mounted open tanks with facilitates for mechanical aeration and/or
circulation were initially used quite successfully.
 Open canvas containers (1m x 1m x 1.25m) are used in Punjab and Madhya Pradesh for
transporting major carp breeders. In those States galvanized iron drums of 180 l capacity are
also used.
 In India, two successful models of closed system of live-fish carrier were designed.
 One is due to Mammen (1962), which he called `Splashless tank’.
 The later model of the splashless tank is of a petrol tanks design of 1,150 l capacity with an
autoclave-type lid.
 It has a built-in aeration system for supplying compressed air, which works on a belt driven
by the engine of the transporting vehicle.
 An oxygen cylinder is carried only as a stand by for emergency.
 The inner surface of the tank is lined with U-foam which prevents physical injury to live fish
during transport.
 A total weight of about 250 kg live fish can be transported at a time in the splashless tank, as
also 90,000 carp fingerlings.
 The load ratio of fish to water in this type of carrier in about 1 kg of fish per 4.5 l water.
 Patro (1968) developed a tank which has an outer chamber of 120 cm diameter open from top
and a slightly smaller one closed from top; the latter, during transport, fits inside the former.
 The top of the inner chamber is provided with an air vent and an oxygen valve.
 The outer chamber serves as a storage tank and is initially filled with water along with fish to
be transported.
 The inner chamber, which is shipped from the upper open end of the water serves as an
oxygen holding chamber at its top and is lined throughout with U-foam to prevent fish from
sustaining injury during transport.
 This double-barrel type carrier as stated by Patro can transport a total weight of 100 kg of live
fish at a time.
3.6. Induced breeding of Indian major carps
 Breeding of fish with pituitary gland (hypophysis) extract is termed as Hypophysation
 The credit for developing the technique of hypophysation in the world goes to the Brazilians,
while the pioneers of hypophysation of Indian major carps are H.L.Chaudhary and
K.H.Alikunhi.
 Induced breeding refers to inducing fish to release gametes through the application of
pituitary extract or hormones or chemicals.
Identification of sex of brooders
 Identification of sex is a prerequisite to induced spawning of the fish.
 Fish is sexually dimorphic and sexual dimorphism is exhibited primarily by gonads and their
ducts and this involves killing of fish.
 Alternatively, the sex is identified based on certain morphological/external characteristics
which include size, length, weight, colouration, fin characteristics, modification in the head in
the form of nuptial dress, genital opening, width of mouth, etc.
 Carps are sexually dimorphic i.e. mature male and female are morphologically different.
 Some of the external morphological characters which are developed during breeding season
could be used to identify sex in major carps which mature during their 2nd
or 3rd
year.
Characteristics Male Female
1. Scale, Operculum and pectoral fins Rough to touch, particularly the dorsal
surface of pectoral
Pectoral smooth to slippery
2. Abdomen Round and firm Swollen and soft
3. Genital opening swollen Elongated slit, white in colour, not
swollen
Round and pink
4. When pressure applied on abdomen
opening
milky white fluid oozes through
genital opening
a few ova may ooze through genital
5. Shape of body and size Body linear, swollen stouter, slightly larger
Male (top) and female (bottom) catla brooders
3.6.1. Breeding technique
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 The male and female brooders are conditioned for a few hours prior to injection.
 Sets of brooders are formed, each consisting of 1 : 2 (female : male) ratio.
 The injected brooders are released in the breeding hapa.
Breeding hapa
 A Breeding hapa is a box-shaped cloth enclosure made of long cloth, generally of size 2 x 1 x
1 m with provision to close its top after releasing brooders.
 The upper flap is attached to one side and the other sides are either tied or buttoned.
 The hapa is fixed in a canal or pond or cement cistern.
 The four bottom and four top corners are tied to four poles such that the bottom of the hapa
should not touch the ground and one-third of the hapa remain above the water level.
Nylon breeding hapas
3.6.1.a. Injection of brooders
1. Intra-muscular injection
 It is administered into the muscle on the caudal peduncle or behind the dorsal fin, but above
the lateral line.
 It is most effective, convenient, simple and less risky.
 It is widely practised.
2. Intra-peritoneal injection
 It is give through the soft regions of the body, generally at the base of the pelvic fin or the
pectoral fin.
 It is risky as it may damage the goads or liver.
3. Intra-cranial injection
 In this method, the injection is given through the cranium and is also risky as it may damage
the brain.
 The pituitary extract is administered through a glass or disposable syringe, 2.0 ml capacity,
having 0.1 ml graduation.
 The size of the needle depends upon the weight of the brooder to be injected.
 Needle number 22 is used for fish weighing 1-3 kg, No. 19 for larger fish and No. 24 for
smaller fish.
 Intra-muscular injection is commonly practiced.
 The hormone injection (pituitary/ovaprim/ovatide) is given at the caudal peduncle region in
between posterior end of dorsal fin and base of caudal fin, above the lateral line, avoiding the
lateral line.
Collection of brooders for injection Selection of catla brooder for injection
3.6.1. b. Spawning
 After releasing the brooders in the hapa, they should not be disturbed.
 After about 6 hours, splashing will commence for breeding and be involved in courtship
which will continue for one hour.
 At the climax of the courtship, both the partners will be seen in an embrace with their
bodies twisted around each other. This exerts pressure on the abdomen, resulting the
extrusion of gametes.
 The following morning, the spent brooders are removed and then the eggs are collected and
transferred for hatching in a suitable hatching device.
3.6.1.c. Examination of eggs
After the eggs are water-hardened, a sample of eggs is taken in a beaker for assessing quality and
quantity.
 The fertilized (good) eggs are transparent with a clearly visible nucleus at the centre and
look-like pearls.
 The unfertilized (bad) eggs are opaque white and the nucleus disintegrate within one hour.
Quantitative assessment of eggs Fertilized eggs circulating in a circular hatchery
3.6.1.d. Fertilization rate
It indicates the quality of developing eggs and is estimated using the formula :
Fertilization rate (%) = No. of fertilized eggs/Total no. eggs x 100
Hatching rate
It can be estimated by knowing the total volume of spawn /number of spawn in a known volume
Hatching rate (%) : Total no. of spawn obtained/Total no. of fertilized eggs x 100
3.7. Induced breeding of exotic carps (silver carp and grass carp)
 The silver carp and grass carp are native to China and hence are known as exotic carps.
 They were first introduced into India in the year 1959.
 Both were successfully induced bred in 1963, through hypophysation.
 The method of hypophysation of these species is similar to that of IMC.
 However, there are a few minor differences – the dosage of pituitary is double compared to
IMC and, unlike IMC, they do not spawn naturally after injection, in captivity.
 The sex of brooder is identified based on the morphological characteristics such as fins,
abdomen, genital opening, etc. The same criteria that are used for IMC can also be used for
silver carp and grass carp.
 The grass carp can spawn naturally after injection if fed on artificial diet, by avoiding plants,
at least two months before spawning.
 To facilitate better fertilization, the eggs need to be stripped by applying pressure on the
abdomen and eggs fertilized by mixing with similarly stripped milt.
Fertilization of eggs
 After hormone injection, the male and female brooders are released separately in breeding
hapas.
 The female brooder is checked for ovulation at 3-4 hours interval.
 After ovulation, the female is stripped and the eggs are collected on to a clean basin/tray,
avoiding any contamination with excreta, mucus, blood, etc.
 Eggs are then mixed with milt stripped from more than one male (preferably 2-3 males) to
ensure better fertilization as these fishes produce comparatively less milt.
 The water-hardened developing eggs are transferred to incubators for hatching.
 The method of hatching of eggs and hatching period are same as that of IMC.
Male (top) and female (bottom) grass carp brooders
3.8. Major carp egg and embryonic developmental stages
 The fertilization of major carp eggs is external.
 The fertilized eggs absorb water and swell considerably in 10-15 minutes and they becomes
a little hard and this process is known as water hardening.
 They are bead-like, non-adhesive, semi-buoyant and have a large perivitelline space.
 The size of the eggs varies between 2.5 and 6.5 mm.
Important major carp egg and embryonic stages and time required for each stage are as follows
Sl. no. Time required at 24-310
C
(Cumulative)
Stage
1 0 min Fertilization
2 10-15 min Water hardening and blastodisc formation
3 30-50 min First cleavage (2 cells)
4 40-65 min Second cleavage (4 cells)
5 50-80 min Third cleavage (8 cells)
6 60-90 min Fourth cleavage (16 cells)
7 70-120 min Fifth cleavage (32 cells)

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8 2-2½ hrs Morula stage – less than half of yolk invaded by germ layer
9 2½-3 hrs Blastula – about half of yolk invaded by germ layer
10 3-3½ hrs Gastrula – about three-fourth of yolk is invaded by germ
layer
11 3-4 hrs Yolk-plug stage – complete invasion of yolk by germ layer
excepting a small pore (blastopore)
12 4-6 hrs Elongation of yolk mass-embryonic stage starts
13 5-6 hrs Pea-shaped embryo – somites and myotomes appear
14 8-10 hrs Comma-shaped embryo- optic vesicles appear, occasional
twitching movement starts, gill rudiments and pectoral fin
bud appear, heart appear
15 14-15 hrs Advanced embryo – increase in the number of somites and
myotomes, twitching movement quite rapid
16 18-20 hrs Hatching – it takes place over a period of 2-3 hrs at 24-310
C
3.9. Causes of mortality of fish eggs and spawn and their treatment
Improper production and delivery of seed to farmers or poor management of broodfish and fish
seed by farmers once stocked, may lead to decline in fish production.
Some of the causes of mortality of fish eggs and spawn are:
 Poor water quality
 Poor pond hygiene
 Presence of Pests
 Poor management of broodfish and seed
 Transportation stress
 Conditioning of fish seed
 Mortality of eggs and hatchlings
 Diseases and parasites
Poor water quality
 It is known that a lot of major carp eggs perish during incubation as a result of heavy
siltation (particularly so during the first few weeks after the onset of monsoon), oxygen
depletion, high hydrogen sulfide level, etc.
 This problem is all the more serious if reservoir water is taken directly to hatching tank.
 Similarly, newly hatched spawn also suffers from the above mentioned problems.
 High density of eggs and spawn also contributes to high mortality, particularly in Chinese
type of carp hatchery where a large quantity of unfiltered water is used.
 Use of filtered water will greatly help reduce mortality of fish eggs and spawn.
Poor pond hygiene
 Mass mortality of seed in carp nursery ponds is frequently encountered in Karnataka
(Mohan and Shankar, 1995).
 Mortality of seed is attributed mainly to prevalence of protozoan parasites, particularly in
ponds which are not dried properly prior to stocking.
 Pond drying followed by liming is known to considerably reduce mortality and improve seed
quality.
Presence of Pests
 The presence of fairly shrimp (Streptocephalus spp) in carp nurseries is known to hamper
the growth and survival by competing with fry for food, space and oxygen.
 Presence of weed fishes in nursery pond leads to the production of stunted fingerlings.
Poor management of broodfish and seed
 Competition among fish seed producers to meet demand some times leads to poor
management of broodfish and fish seed may negatively affect seed quality.
 Substandard quality seed is frequently observed as a result of high stocking density in
nurseries.
 Fish hatcheries in India are concerned more about the quantity rather than the quality of
fish seed and produce them without following any selection norms.
 Consequently, the seed suffer from high rates of mortality, poor growth and are prone to
diseases and parasites.
Transportation stress
 During transportation fish seed is subjected to confined environment, higher metabolic load,
stress, strain and exhaustion. As a result, the seed becomes susceptible to diseases and
parasites.
 Chowdhury (1996) used scale loss and tail damage of carp after transportation as quality
indicators.
Conditioning of fish seed
 Conditioning is acclimatizing seed to a restricted environment prior to packing and
transportation.
 During this period the seed is stocked at a very high stocking density in a hapa or a pond
with running water, but without provision for food so that the weak seed dies and only the
healthy fry survives.
 The seed that survives the `stress test’ only is selected for transport to a required
destination. This type of conditioning of fish seed is commonly practiced in several States of
India.
Mortality of eggs and hatchlings
 Fish farmers in West Bengal at times encounter heavy mortality of eggs and hatchlings
during incubation period.
 This has been found to be due to immature bursting of egg shells and release of premature
hatchlings before the anticipated period of hatching. Such hatchlings either do not survive or
suffer mortality at subsequent stages.
 To overcome this problem, fish farmers of the State have been using a solution which is a
mixture of extract of catechu (Acacia catechu) and Myrobalan (Myrobolus indica).
 The plant extract enhances hatching period and prevents hatchlings from premature release
due to the presence of tannin which helps harden the egg membrane (chorion).
Diseases and parasites
 High stocking density, artificial feeding, water fertilization, etc. have become common
husbandry practices in carp nursery and rearing systems to optimize returns.
 These high density systems offer the ideal environment for disease outbreak because such
systems have stressed host and virulent pathogen.
 Depending on the nature and severity, the disease may cause mass mortality of the affected
population in a short time, produce protracted small scale mortality, reduce growth, make
the larvae unsuitable for stocking.
 The need for adopting suitable health management measures to reduce the loss due to
diseases is being increasingly felt by hatchery operators.
3.9.1. Diseases and parasites in carp hatcheries
The nursery and rearing systems of carps are often very rich organically and provide an ideal
environment for many of the pathogens. Important problems in hatcheries and early rearing
systems are caused by some of the following pathogens:
1. Protozoan ciliates like Ichthyophthirius multifilis (white spot disease), Trichodina complex and
ectodermal ciliates like Epistylis, Vorticella. All these ectoparasites can cause mass mortality of
younger stages of carp very quickly and the situation becomes worst in waters with low oxygen and
high organic matter.
2. Disease caused by Myxosporidians are a serious threat to the fish seed farms. These sporozoan
spores present in the pond soil are normally ingested by the developing fry. Once inside the target
tissue the sporozoans cause massive destruction of their target tissue and produce large scale
mortality.
3. Worm parasites like Dactylogyrus (gill fluke) and Gyrodactylus (skin fluke) with their well
developed attachment haptor and feeding apparatus can cause mortality in early developmental
stages of carps.
4. Opportunistic secondary bacteria (Aeromonas) and fungi (Saprolegnia) can become serious
problem in fish larvae which are heavily parasitized.
In carp rearing ponds major problems are because of larger ectoparasites, secondary invaders,
systemic bacterial pathogens and viruses. Ectoparasites like Dactylogyrus and Argulus (fish lice) and
endoparasites like sporozoans are very important. Bacterial problems like surface ulcerative
conditions and acute systemic diseases are common in carp rearing systems. Many a times
mortalities seen in carp culture systems are a result of ectoparasitic and systemic bacterial diseases.
External fungal problems are normally associated with fish which are poorly handled. The possibility
of viral diseases causing mortalities in carp nurseries cannot be ruled out.
3.9.2. Bacterial and fungal diseases in carp rearing systems
 Bacteria can cause diseases either as secondary invaders or as primary pathogens.
 Bacterial diseases in larvae can be broadly classsified as surface ulcerative, acute systemic
and chronic granulomatous type.
 Surface ulcerative type of diseases are characterized by haemorrhagic surface ulcers and are
normally caused by Aeromonas, Pseudomanas, Vibrios, Flexibacteria, Myxobacteria, etc.
Surface ulcerative disease conditions at times develop to acute systemic disease.
 These are characterized by the presence and proliferation of bacteria in internal organs like
kidney, heart, spleen, blood and other visceral organs.
 These diseases produce significant necrotic changes in all the affected organs and can cause
mortality in a short time scale.
 Bacterial haemorrhagic septicaemia caused by numerous serotypes of Aeromonas
hydrophila is a major problem.
Treatment chart for common disease conditions of carp larval rearing systems
Disease agent Chemical Method Concentration/time
1. Ectoparasitic protozoans
a. Ichthyophthirius sp. Formalin Short bath 60-100 ppm for 30 minutes
b. Trichodina sp. Formalin Long bath 20-30 ppm
c. Epistylis & Vorticella Formalin Dip 200-300 ppm for 1 minute
d. Ichthyobodos sp. Formalin Dip 20-30 pm for 30 minutes
2. Monogenetic worms
a. Dactylogyrus Organophoshporus
pesticides
Dip 10 ppm for < 1 minute
b. Gyrodactylus Nuvan, Dipterex Long bath 0.5 ppm for 24 hours
3. Crustaceans
a. Lernaea Nuvan, Dipterex Long bath 0.5 ppm for 24 hours
b. Argulus Nuvan, Dipterex Long bath 0.5 ppm for 24 hours
4. Endoparasites
a. Sporozoans Nuvan, Dipterex Long bath 0.5 ppm for 24 hours
b. Cercaria and metacercaria
of digenetic trematodes
Nuvan, Dipterex Long bath 0.5 ppm for 24 hours
5. External Mycosis –
Saprolegnia
Malachite green
Formalin
Dip
Bath
Bath
60 ppm for < 1 minute
1-2 ppm for 1 hr
50-75 ppm for 30 minutes
100-200 ppm for 1-3

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This document provides instructions for breeding and raising common carp. It details the process of selecting and transporting mature male and female carp, injecting them with hormones to induce spawning, collecting and fertilizing the eggs, incubating the eggs and hatching fry. It also describes transferring fry to nursery ponds, feeding them emulsified egg yolk initially and then ground feed. Water quality is monitored throughout the growing process until the fish reach market size.

Dip
minutes
6. Surface bacterial diseases Proflavine
Oxytetracycline &
Furnace
Bath
Short bath
20 ppm
1-5 ppm
7. Systemic bacterial
diseases
Furazolidone
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In feed
In feed
In feed
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10mg/kg fish/day
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symptoms of the disease are the presence of gas bubbles in gill filaments, heart and blood vessels
and also in the gut. The air bladder gets highly distended. The `gas disease’ also referred to as ‘gas
embolism’ occurs due to supersaturation of water with either oxygen or nitrogen and is generally
encountered in fish nurseries.
Unit 4 - Different types of fish hatcheries
4.1. Hatcheries
 Presently, hatcheries account for the lion’s share of India’s fish seed production.
 The country’s fish seed production which was only 211 million fry in 1964-65, increased to
20,700 million fry in 2008.
 A quantum jump in fish seed production from the 1980s is attributed mainly to the
introduction of the technology of the Chinese type of carp hatchery and refinement of the
technology of induced breeding, coupled with usage of ready-to-use fish spawning agents
like ovaprim. However, India still faces shortage of quality fish seed.
 West Bengal, where inland fish culture and fish seed production is an age-old practice,
continues to be ranked first in fish seed production and supply, far ahead of the 2nd
ranked
State i.e. Assam, where there is a great demand for freshwater fish.
Figure: India’s fish seed production statistics (from 1964-65 to 2003).
4.1.1. Hatchery proper
 The evolution of fish (carp) hatchery systems in India was reported by Dwivedi and Zaidi
(1983).
 Several types of fish hatchery devices are in vogue in India.
 These range from the simplest hatching pits to the most modern.
1. Hatching pits
 The earliest device of hatching carp eggs, used in bundh-type breeding system of Bengal,
comprised pits.
 The dimensions of the pits vary according to the requirement, but pits of 3’x 2’x 1’ are dug in
multiple rows and their inner walls are plastered with mud.
 About 30,000-40,000 eggs can be kept for hatching in each pit.
2. Chittagong type hatchery pits
 These are similar to hatching pits except that they are each provided with a double-walled
(inner mosquito netting material and outer muslin cloth) cloth linings.
 The outer cloth is kept a few inches above the earthen bottom of the pits.
3. Earthen pot hatchery
 This is one of the earliest types of hatchery used in association with bundh-type breeding
system.
 It comprised earthen pots arranged at different levels one draining into the other kept at a
lower level.
 This provides a flowing current of water, cooled by surface evaporation of the porous
earthen pots in which the carp eggs are hatched.
4. Double-walled hatching hapa
 The double-walled hatching hapa is one of the commonest devices to serve as an outdoor
hatchery.
 Installable in a pond or in the margin of a river, up to 1,00,000 eggs can be hatched in the
inner mosquito net wall of the hapa of the size 1.75m x 0.90m x 0.75m.
 The newly hatched hatchlings wriggle out through the round meshes of the inner wall and
collect themselves in the outer whole cloth enclosure
 Larval survival is much higher when the hatching hapa is installed in a gently or fast flowing
canal or a river because of superior exchange of water.
5. Floating hapa
 Similar in principle to the double walled hatching hapa, a floating hapa is mounted on a
wooden frame to which it is securely tied such that the whole unit drifts in water.
 The floating hapa has the advantage that it can be used on rocky substrates, often found in
reservoirs and can be towed to deeper water to obtain better exchange of water.
6. Tub hatchery
 First introduced in the Madhya Prudish State.
 This hatchery furnishes running water to hatch eggs in galvanized iron units with adjusted
levels such that water flows by gravity through interconnecting siphons.
 Each tub (2.5’ x 2.5’x 1.5’) is fitted with double walled hapas and the eggs are constantly
bathed in a gently flowing water current.
7. Cemented cistern hatchery
 Used at Nowgong dry bundh in Madhya Pradesh and is situated generally below the dam
sites
 Each cement cistern (tank) commonly measures 2.4 x 1.6 x 0.45m.
 It has its own inlet, situated at different levels at the opposite ends of the cistern.
 They are often arranged in rows wherein water flows by gravity and each cistern can hatch
about 3,00,000 eggs at a time.
8. Glass jar hatchery
 The credit of developing India’s first transparent hatching device in which the developing
eggs can be watched at eye level goes to Bhowmick.
 In this system, the hatching are automatically transferred to storage hapa/spawnery, within
the hatchery building itself.
 The main components of Bhowmick’s glass jar hatchery are : an overhead tank, fish breeding
tank, incubation/hatching jars and a spawnery to hold the newly hatched spawn.
 The capacity of the overhead tank is 5,500 l and that of each of 20/40 hatching jars is 6.35 l.
 The spawnery comprises two cement tanks (1.8 x 0.9 x 0.9 m each) which can hold a nylon
hapa measuring 1.65 x 0.8 x 1.0m, projected above the tank and hence deeper than the tank
and has an overhead shower for spray.
 Each jar can accommodate 50,000 water hardened and swollen eggs at a time. The water
flow rate maintained is 600-800 ml/minute for IMC.
 This was earlier a very successful system, but the hatchery jars are now obtained against
orders only.
9. Transparent polythene jar hatchery
 This hatchery is identical to Bhowmick’s glass jar hatchery except that the breakable
hatching jars are replaced by transparent polythene containers.
 Each jar is provided with an inlet pipe and an outlet pipe and escape of eggs from jars is
further prevented by an inner mesquite netting container.
 Each jar has its water supply inlet pipe going down almost up to the jar bottom which is of
concave type, in hatchery jars.
 The water on reaching the jar bottom is reflected upwards till it finds its exit located at the
top of the jars.
 The water in a jar of this type develops greater churning and suspended eggs therein get
better oxygenation.
 This is perhaps a superior system than the unidirectional flow pattern of Zoug jars

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10. Galvanized iron jar hatchery
 This hatchery resembles Bhowmick’s glass jar hatchery except that the hatchery glass jars
are replaced by galvanized iron jars.
 Each jar has a cylindrical portion (48.5 cm long and 23.0 cm diameter ) and a top conical
portion (19 cm long).
 The jars are filled on an angle iron framework which also accommodates a galvanized iron
conduct (10 cm wide and 10 cm high) to carry the hatchlings from the jars to the spawnery.
 The water flow rate is 1 L/min/jar. The galvanized iron jar hatchery is cheaper than glass jar
or polythene jar hatchery.
11. Shirgur’s bin hatchery
 This hatchery consists of a rectangular aluminum container (54’’ x 16’’ x 22’’) provided with
circulating water (243 L/min) in which are placed cylindrical egg vessels (12’’ diameter and
12’’height).
 Each egg vessel can hold about 200,000 carp eggs at a time.
12. Hanging dip net hatchery
 This hatchery comprises conical 1/16’’ cloth dip nets (65 cm diameter at top and 46 cm
diameter at bottom) provided with a 50mm brass spray head at the bottom of each dip net.
 Such dip nets are hung in hatching tanks of 3.3 x 1x 1m.
 The water flow rate is 1-1.5 l /min during hatching and about 100,000 eggs can be hatched in
each container.
 After hatching, the hatchlings pass through the meshes of the dip net and get collected in
the tank where they are allowed to remain for 3 days before being transferred to nursery
pond.
 Such units were installed in Orissa State.
13. Circular cistern hatchery
 This consists of a galvanized iron circular cistern (tank) of 1 m3
capacity with a series of inlet
facets placed at 45o
at a height of 5 cm from ground level such that when connected to
water supply, water moves in a circular fashion.
 The overflowing water is allowed to leave the cistern from an outlet placed at the top by a
monofilament 60 mesh/ linear inch.
 The hatchlings are left behind in the cistern till their transfer to nursery pond.
14. Chinese type of hatchery
 To the Chinese goes the credit of developing the concept of circular breeding and hatching
tanks in which water shows circular or centrifugal motion.
 Such hatcheries used for hatching carp eggs in India are of Chinese origin and they are called
as the Chinese type of carp hatcheries.
 This system, within a small space, simulates some aspects of riverine environment and has
proved itself a very successful method of breeding carps where commercial production of
carp seed is required.
 In this system, the outlet lies in the middle of the circular tank guarded by a circular
perforated structure or a sloping outlet.
 The principle of a hatching tank is similar to that of a breeding tank, excepting that the
former is smaller and normally has two chambers, giving the shape of a double doughnut to
the hatching tank.
 The outlet lies in the middle of the circular tank guarded by a straight, circular perforated
pipe which regulates water level in both the chambers.
 One wall of the double doughnut lies at the periphery and the other at the inner end
surrounding the outlet.
 The water circulates between the two walls in a circular fashion, with the help of water jets/
inlets, placed at 45 degree from the bottom.
 It is where the eggs are hatched.
15. Low density polyethylene (LDPE) hatchery
 S.N. Dwivedi, a former Director of Central Institute of Fisheries Education, Bombay was
instrumental in introducing LDPE material in fish hatcheries.
This material, like moulded plastic, is made in one piece, has no joints or welds.
 This hatchery comprises overhead tanks, cooling tower and compressors. It combines
breeding and hatching facilities.
 Each vertical hatching jar of capacity 40 L can hold 0.2-0.25 million developing eggs.
In earlier days, fish hatchery was used as a facility for hatching of fish eggs collected from rivers and
bundhs. At that time, rivers and bundhs were the main sources of carp seed.Over the years the
development and refinement of the technique of induced breeding has been enlarged. Hence more
emphasis is being given to the hatchery proper for the large scale production of fish fry.
Details on the number of Indian major carp hatcheries (both public and private sector), spawn
production, conversion rate from spawn to fry, type of hatchery, spawning agent used, etc. are
presented below. It is evident that the Chinese type of circular hatchery is the most widely used
hatchery for large seed production all over the country. Jar hatchery (glass or fiberglass) and double-
walled hatching hapa are used for medium or small scale operation in some parts of the country.
Among the different fish spawning agents, ovaprim, a ready-to-use agent, has been the most
popular hormone among fish hatchery operators.
4.1.2. Indian major carps (indicative spawn production)
State
Number of
hatcheries
Spawn
production
(lakhs p.a.)
Spawn to fry
conversion
rate
Type of
hatchery
Spawning agent
used
Andhra
Pradesh
20 55,000 30% Jar/circular
(Chinese type)
Pituitary extract/
ovaprim
Arunachala
Pradesh
1 n.a. Circular n.a.
Assam Circular Pituitary extract/
ovaprim
Bihar 4 3,300 <25% Circular Pituitary extract/
ovaprim
Gujarath 12 2,200 25% Circular Pituitary extract/
ovaprim
Haryana 21 5,150 25% Hapa, circular,
jar
Pituitary extract/
ovaprim
Karnataka 28 6,343 20% Circular, jar,
hapa
Pituitary extract,
ovaprim, HCG,
ovatide
Kerala 28 21,000 20% Jar, circular, Pituitary extract/
ovaprim
Madhya
Pradesh
72 15,800 30% Circular,bundhsOvaprim/ovatide
Maharastra 28 10,655 30% Circular, hapa Ovaprim/ovatide
Manipur 4 160 n.a. Circular Ovaprim,
pituitary extract
Orissa 37 19,672 30% Circular Pituitary extract/
ovaprim,ovatide
Punjab 6 950 30% Circular Ovaprim,ovatide,
pituitary extract
Rajasthan 19 6,550 n.a. Circular Ovaprim, ovatide,
pituitary extract
Tamil Nadu 84 8,968 n.a. Hapa, circular Ovaprim,
pituitary extract
Tripura 5 2,960 Ovaprim,
pituitary extract
Uttar Prudish 45 11,970 Upto 50% Circular Ovaprim/pituitary
extract, HCG
West Bengal 30 33,600 Over 30% Circular Ovaprim, ovatide,
pituitary extract,
HCG
Total 420 342,918
Table: Indian major carps (indicative spawn production)
n.a.: data not available; data of Assam State not included in the totals;
* There are/appears to be more carp hatcheries, particulars of which could not be obtained.
Magur, trout and mahseer hatchery details are given in Tables , respectively.
However, the seed of these fishes is not produced on a large scale, barring mahseers.
4.1.3. Other fish spawn production
Magur (Clarias batrachus) hatcheries
state
Number of
hatchery
Spawn
production
(lakhs p.a.)
Spawn to fry
conversion rate
Type of hatchery
Mandhya Prudish 2 1 - -
Orissa 1 - - Experimental
West Bengal 1 - - Experimental
Total 4 1 - -
Trout hatcheries
State Number of hatchery
Spawn production (lakhs
p.a.)
Spawn to fry conversion
rate
Arunachal Pradesh 1 n.a. n.a.
Jammu and
Kashmir
3 6 n.a.
Himachal Prudish 4 30 n.a.
Tamil Nadu 1 n.a. n.a.
Uttar pradesh 3 1.30 n.a.
Total 12 37.30
Source : Fishing Chimes, 19 (10 & 11) : 212-213
Mahseer hatcheries
State Number of
hatcheries
Spawn
production
Spawn to fry
conversion
Type of
hatchery
Spawning agent

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(lakhs p.a.) rate
Maharashtra 1 5 n.a. Hatching
trays
Natural
(stripping)
Karnataka 1 10 n.a. Hatching
trays
Ovaprim,ovatide
Uttaranchal 2 n.a. n.a. Hatching
trays
n.a.
Jammu and Kashmir 1 10 n.a.. Circular n.a.
Kerala 1 n.a n.a. Hatching
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Tamil Nadu 1 Under
construction
n.a. n.a. n.a.
4.2. Chinese type of carp hatchery
 Hatching and breeding devices of circular shape and are of Chinese origin are referred to as
Chinese carp hatchery.
 A hatchery proper is the most essential component of the modern fish seed farm.
 It is here that fish are bred, eggs hatched and hatchlings produced.
 Further rearing can even be done outside, but according to the latest concepts of a hatchery,
up to fingerling rearing is done in the hatchery or the fish seed farm.
 A modern hatchery which incorporates all the essential components and where ecological
conditions are simulated is sometimes referred to as eco-hatchery.
The components of a hatchery proper are
1. Ante-tank,
2. Fish breeding tanks,
3. Hatching tanks and
4. Larvae holding tank or spawnery.
 Ante-tank: The purpose of these tanks is to temporarily hold selected broodfish and to
acclimatize them prior to injection and they are normally located inside the hatchery
building.
 The ante-tanks are normally twins - one each for female and male. A 200 m2
(10m x 20m x
1.5m)ante- tank, divided into two identical twins of 5m x 20m can hold 25 sets of broodfish
comprising female to male in the ratio of 1:2 i.e. 75 specimens of fish.
 Besides holding broodfish for conditioning, the ante-tanks are also used, in modern
hatcheries, for stocking fry or fingerlings prior to sale and also for treatment of diseased or
infected fry, fingerling and broodfish. The ante-tank is generally a concrete structure.
 Depending upon the system adopted, the same breeding tank can serve as a hatching as well
as larval rearing tank for about 3 days after hatching.
 The hatching tank can itself serve as a larval rearing tank besides a hatching tank or can in
turn lead to a larval rearing tank, where the larvae after yolk-sac absorption are given their
first external larval food before being transferred to the outside nursery pond.
The merits: The important merits of this system are that it stimulates some characteristics of
a riverine habitat where the fish naturally belong, very efficient hatching (almost100%),
combines breeding, hatching and larval rearing and suitable for commercial scale operation.
The demerits: Requirement of water is high and in many a hatchery water is in short supply
and a large breeding tank is ill- utilized for small scale breeding operations. Further, concrete
structures are expensive to install and once installed, subsequent modification becomes
virtually impossible.
4.2.1. Infrastructure of eco – hatchery complex for carp seed production (Chinese type)
 For commercial success of fish hatcheries, infrastructural facilities are prerequisites.
 Due to lack of infrastructural facilities, realization of fish seed in nursery/rearing ponds is poor
in many states.
 Modern carp eco-hatchery is the most appropriate system to produce seed of Indian major
carps and exotic carps.
 It is an integrated one, with infrastructure for broodfish care, breeding tank,
hatching/incubation tank, spawn and fry rearing, packing and marketing of seed, water supply
system and buildings.
Top view of a Chinese type of circular breeding tank, with an overhead shower
A view of a Chinese type of circular hatching tank, with the inner chamber covered with nylon
netting (left) and a battery of circular hatching tanks in operation (right).
4.3. Glass jar hatchery
 The credit of developing India’s first transparent hatching device in which the developing
eggs can be watched at eye level goes to Dr Bhowmick.
In this system, the hatching is automatically transferred to storage hapa spawnery, within
the hatchery building itself.
 The main components of Bhowmick’s glass jar hatchery are : an overhead tank, fish breeding
tank, incubation and hatching jars and a spawnery to hold the newly hatched spawn.
 The capacity of the overhead tank is 5,000 l and that of each of 20/40 hatching jars is 6.35 l.
 The spawnery comprises two cement tanks (1.8 x 0.9 x 0.9 m each) which can hold a nylon
hapa measuring 1.65 x 0.8 x 1.0m, projected above the tank and hence deeper than the tank
and has an overhead shower for spray.
 Each jar can accommodate 50,000 water hardened and swollen eggs at a time. The water
flow rate maintained is 600-800 ml/minute.
 This was earlier a very successful system, but the hatchery jars are now obtained against
orders only.
4.4. Transparent polythene jar hatchery
 This hatchery is identical to Bhowmick’s glass jar hatchery except that the breakable
hatching jars are replaced by transparent polythene containers.
 Each jar is provided with an inlet pipe and an outlet pipe and escape of eggs from jars is
further prevented by an inner mosquito netting container.
 Each jar has its water supply inlet pipe going down almost up to the jar bottom which is of
concave type, in hatchery jars.
 The water on reaching the jar bottom is reflected upwards till it finds its exit located at the
top of the jars.
 The water in a jar of this type develops greater churning and suspended eggs therein get
better oxygenation.
 This is perhaps a superior system than the unidirectional flow pattern of zoug jars.
Indoor transparent (left) and outdoor fiberglass jars (right) used for hatching major
carp eggs
4.5. Galvanized iron jar hatchery
 This hatchery resembles Bhowmick’s glass jar hatchery except that the hatchery glass jars
are replaced by galvanized iron jars.
 Each jar has a cylindrical portion (48.5 cm long and 23.0 cm diameter ) and a top conical
portion (19 cm long).
 The jars are filled on an angle iron framework which also accommodates a galvanized iron
conduct (10 cm wide and 10 cm high) to carry the hatchlings from the jars to the spawnery.
 The water flow rate is 1 L/min.
 The galvanized iron jar hatchery is cheaper than glass jar or polythene jar hatchery.
Unit 5 - Fish seed rearing techniques
5.1. Fish spawn rearing techniques – nursery pond
In India, carp culture is carried out in three phases (three-tier system) comprising:
1. Nursery phase (rearing three-day-old spawn to fry),
2. Rearing phase (rearing fry to fingerling stage) and
3. Grow out phase (rearing fingerlings to adult stage).
Of the three phases the nursery rearing is crucial and needs greatest attention of fish hatchery
managers.
 Jhingran and Pullin (1985, 1988) have reviewed the subject of nursery and rearing pond
management.
The allocation of land for different types of ponds and other purposes is shown below :
under Indian conditions a 4 ha farm should be divided into nursery ponds, 0.2 ha; rearing
ponds, 0.8 ha and stocking ponds, 3 ha.
 Considering the enhanced rate of stocking and survival of spawn, fry and fingerlings, the
ratio of nursery to rearing to stock ponds should be 1:40:1280.
 The preparation of nursery, rearing and stocking ponds before releasing the stocking
material is an important step for successful rearing of carp spawn to fry, fry to fingerlings
and fingerlings to table-sized fish.
 The occurrence of large scale mortality of stocked spawn in unprepared nurseries is a
common experience of fish culturists throughout the country.
 Basavaraja and Joseph Antony (1997) obtained survival as high as 100% in the IMC nursery.

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Finfish breeding and hatchery management pdf

  • 1. FINFISH BREEDING AND HATCHERY MANAGEMENT Unit 1. Freshwater and marine fish seed resources and natural breeding Freshwater fish seed resources  Rivers were the major source of freshwater fish seed in India during 1950’s and 1960’s.  Over the years, the riverine contribution has declined and at present forms only a supplementary source, constituting less than 5% of the country’s total fish seed production.  The Ganga, the Brahmaputra and the Indus river systems in the North and the Peninsular East coast and the West coast river systems in the South are the important natural sources of fish seed. 1.1.1. The Ganga river system  The Ganges river system supports diverse freshwater fish fauna ranging from the cultivable Gangetic (major) carps to mahseers and other coldwater fishes of the Himalayas The other resources includes hilsa, catfishes and scampi (Macrobrachium rosenbergii) are of considerable commercial importance. The Principal freshwater fishery resources of the Ganga : Indian major carps: Mahseer: Catla catla Tor putitora Labeo rohita T. mosal
  • 2. Cirrhinus mrigala T. tor Labeo calbasu Acrossocheilus hexagonolepis Other carps: Larger catfishes: Labeo pangusia Osteobagrus aor L. dero O. seenghala L .gonius Silonia silondia Cirrhinus reba Wallago attu Pangasius pangasius Other catfishes: Bagarius bagarius Clupisoma garva Rita rita Eutropiichthys vacha Feather backs: Ailia coila Notopterus notopterus Ompok bimaculatus N. chitala O. pabda Clupeids: Freshwater prawns: Hilsa ilisha Macrobrachium malcolmsoni Gudusia chapra M. birmanicum Setipinna phasa M. lamarei 1.1.2. The Brahmaputra system The middle and lower stretches have several species of carps, catfishes and air breathing fishes and also the anadromous hilsa. The Principal freshwater fishery resources of the Brahmaputra : Major carps: Catfishes: Catla catla Silonia silondia Labeo rohita Osteobagrus aor Cirrhinus mrigala O. cavasius
  • 3. Labeo calbasu Bagarius bagarius Other cyprinids: Mahseers: Labeo gonius Tor putitora L. bata T. progenius Danio equipinnatus Murrels: Rasbora daniconius Channa punctatus Puntius sarana C. marulius P. ticto C. gachua Barilius bendalensis Other fishes: Chela atpar Rhinomugil corsula Clupeids: Glassogobius giuris Hilsa ilisha Colisa lalia 1.1.3. The Indus system  The Indus river has the exotic rainbow trout and brown trout in the upper reaches and a wide variety of indigenous carps and catfishes in the lower stretches.  The trout streams of Kashmir constitute one of the world’s richest sport fishing waters attracting anglers and tourists from world over. • Harbors the exotic rainbow and brown trout, variety of indigenous carps and catfishes • The trout streams of Kashmir - one of the world’s richest sport fishing The Principal freshwater fishery resources of the Indus: Carps: Catfishes: Cyprinus carpio Glyptothorax kashmirensis Schizothorax sp G.reticulatum Oreinus lagiostomus Osteobagrus seenghala Labeo dero L. dyocheilus Other fishes: Puntius conchonius Botia birdi
  • 4. Crossocheilus latius Nemacheilus kashmirensis Tor putitora N. rupicola N. marmoratus 1.1.4. The East Coast system The east coast river system in Peninsular constitutes the Mahanadi, the Godavari, the Krishna and the Cauvery.  The Mahanadi has similar resources as in Ganges system including all the Indian major carps.  The other rivers, besides their own indigenous fish fauna of several carp species, catfishes, murrels, prawns, etc. have had their water enriched by repeated transplantation of the Gangetic carps from the North.  The transplants have established themselves and contributed significantly to the fish fauna of these rivers.  The tributaries of the Cauvery from the Nilgris have coldwater fishes like trout and tench . The Principal freshwater fishery resources of the Mahanadi: Carps: Catfishes: Catla catla Osteobagrus seenghala L. rohita O. aor C. mrigala Rita rita L. fimbriatus R. chysea Mahseers: Tor mosal The Principal freshwater fishery resources of the Godavari: Carps: Catfishes: • L. fimbriatus Osteobagrus seenghala • C. mrigala O. aor • L. calbasu Silonia childreni • Catla catla Wallago attu • L. rohita Pangasius pangasius Bagarius bagarius • Hilsa ilisha • Macrobrachium malcolmsoni
  • 5. The Principal freshwater fishery resources of the Krishna: Indian major carps: Catfishes: • C. catla Osteobagrus seenghala • L. rohita O. aor • C. mrigala Wallago attu • Ompok bimaculatus Other carps: Murrels: • L. fimbriatus Channa marulius • L. kontius C. striatus • P. sarana • P. dubius The Principal freshwater fishery resources of the Cauvery: Major carps: Catfishes: • C. catla Glyptothorax madrapatanus • L. rohita Osteobagrus seenghala • C. mrigala O. aor • L. calbasu Wallago attu Minor carps : Silonia silondia • P. carnatius Other fishes: • L. kontius Channa marulius • C. reba Notopterus notopterus • C. cirrhosa Tor khudree • P. dubius v) The West Coast river system  The West Coast river system includes the basins of the Narmada and the Tapti which supports varied fauna of commercial importance.  The other rivers that originate in the Western Ghats possess carps, catfishes, mahseers, murrels, perches, prawns, etc. The Principal freshwater fishery resources of the Narmada: Indian major carps: Catfishes: Catla catla Rita pavimentata L. rohita Osteobagrus seenghala C. mrigala O. aor L. calbasu Wallago attu
  • 6. Clupisoma garua Other carps: O. bimaculatus L. fimbriatus Mystus cavasius L. bata L. gonius Miscellaneous fishes: C. reba Channa sp P. sarana Mastocembelus sp N. notopterus Tor tor The Principal freshwater fishery resources of the Tapti: Indian major carps: Other carps: L. calbasu L. fimbriatus C. mrigala P. sarana Catfishes: L. boggut Osteobagrus seenghala L. bat O. aor Tor tor Wallago attu C. reba Clupisoma garua Other fishes: Channa sp Mastocembelus sp 1.1.6. Other freshwater resources Reservoir fish seed resources  The reservoirs in Uttar Pradesh and Madhya Pradesh, by virtue of their being connected with the Ganga river system, have a natural stock of major carps.  But in view of a large volume of water impounded by them, the original stock is being supplemented by stocking them regularly with major carp fingerlings.
  • 7.  The reservoirs across other basins, however, do not have a natural stock of major carps. Hence, major carp fingerlings produced elsewhere were brought and released in them. Cultivable sp 1.1.7. Coldwater fish seed resources  The optimum range of temperature for cold water fish is 10-120 C.  In India, lakes and streams located at more than 900 m above mean sea level qualify for coldwater. Trouts, salmon and mahseers are the typical cold water fishes in India.
  • 8. Trout, salmon and char  Trout and salmon are the only exotic game fishes introduced in India. The species of trout introduced are rainbow trout, brown trout, eastern brook trout, golden rainbow and tiger trout (a hybrid between Salmo trutta fario x Salvelinus fontinalis).  In the Peninsular India trout has been introduced in the Nilgiris and Kodai hills in Tamil Nadu and in the high ranges of erstwhile Travancore in Kerala.  In the Himalayas, trout has been introduced in Kashmir and Himachal Pradesh, in Garhwal Himalayas, Arunachal Pradesh, Nagaland, Meghalaya and in certain waters of Nepal. Mahseers  The mahseers are regarded as a sacred fish by the Hindus.  They are distributed in streams, rivers and lakes in the hilly regions of the Himalayas and the Peninsular India. They are found in the plains where water is cool, clear with high oxygen content.  There are seven species of mahseer belonging to the genus Tor in India. They are: Tor tor, T. putitora, T. khudree, T. nelli, T. progenius, T. mussullah and T. mosal. Mahseer fishery resources are protected in temple pools by Temple authorities Snow trout  Snow trout (Schizothorax spp.) are believed to have migrated into the lakes and streams of Kashmir from Central Asiatic watersheds.  Most of them are now regarded as endemic to the Kashmir valley.  Presently, there are eleven valid species of Schizothorax. Schizothoracids collected from different lakes and streams have been successfully induced bred. Hilsa, Tenualosa ilisha  The anadromous Indian shad, Tenualosa ilisha (Hamilton), commonly known as hilsa or river shad is, undoubtedly, one of the most commercially important fish of the country.  Hilsa ascends the freshwater stretch of all the major river systems from sea mainly for breeding, thereby forming a lucrative fisheries in freshwater and brackishwater.  It’s upstream migration has greatly been hampered by the construction of dams, weirs and barrages across the rivers. 1.2. Marine fish seed resources This seed resource comprises of brackishwater and marine fish species. India is bestowed with several species of finfish along its 8100 km coastline. The most important fish seed resources are Milkfish: It is distributed in the Indo-Pacific region. Milkfish is found along both the coasts of India. Primarily milk fish is marine species but it ascends to freshwater zones of river. Grey-mullets: They are distributed both on the West and East coast of India. They are more commoly found in the estuarine regions. Seabass: It occurs in the tropical and sub-tropical areas of Asia in coastal waters, estuaries and lagoons, including freshwater.
  • 9. Pearlspot: Three species of pearlspot inhabit brackishwater and mouths of rivers in Orissa, Andhra Pradesh, Tamil Nadu, Kerala ands Karnataka. Sillaginids: They are found in the Indo-Pacific region. In India, they occur in shallow, sandy bottoms of shores and bays and also in estuaries. Polynemids: They are distributed in the Indo-Pacific region. They occur along both the coasts of India. They primarily inhabit shallow muddy and sandy bottoms of the continental shelf, occasionally entering rivers. 1.3. Natural breeding of finfishes  Most teleosts are seasonal breeders and their spawning coincides with seasonal changes in environmental factors.  In response to environmental stimuli, the hypothalamus secretes a hormone termed as releasing hormone (RH).  The Nucleus Lateralis Tuberis (NLT) of the hypothalamus responds to an electrical signal from the brain by secreting RH at the end of the axon; thus an electric signal becomes a chemical one (bridging the gap between nervous/neural and hormonal information).  The arrangement of these neurons/axons is unique in teleosts, their axon directly end on gonadotropic cells in the adenohypophysis, but do not secrete their hormones into a portal blood system.  However, the effect of RH is to stimulate the production of Gonadotropic hormone (GtH) and its subsequent release into the vascular system of the adenohypophysis.  GtH is then carried in the general blood circulation to the gonads which in turn secrete steroids which are required for final maturation and spawning. The chain of events leading from the reception of environmental stimuli to maturation and release of gametes is as follows: 1.3.1 Feedback mechanism  The level of gonadotropins (GtH) is regulated by a process called feedback mechanism.
  • 10.  It is believed that the release of GtH is adjusted through a system of negative feedback, in which centers in the pituitary/hypothalamus are responsive to the level of circulating gonadal steroids.  A rise in the level of sex steroids brings about a decrease in GtH secretion; with the result the steroid release again falls to the appropriate level.  A drop in the steroid level has the opposite effect (a decrease in the steroid level brings about an increase in the level of GtH).  In special cases, an antiestrogen or antiandrogen competes with endogenous gonadal steroids for binding sites in the P-H (pituitary-hypothalamus) axis so that GtH is released regardless of the level of steroids.  A negative feedback system becomes then a positive one and results in an artificially elevated level of gonadal steroids. So both the GtH and gonadal steroids continue to rise and the pathway becomes positive. 2. Selection of riverine fish spawn collection sites, gear used and methods of collection  The Central Inland Fisheries Research Institute (CIFRI) located at Barrackpore, Kolkata, during 1959 – 1964, conducted a pioneering program of seed prospecting investigations on various river system with a view to ascertaining the quality and quantity of fish seed, availability, gears for spawn collection, method of collection, measurement of fish seed, factors responsible for fluctuation in seed availability, etc. on an all-India basis.  The diverse geographical and climatic conditions of India greatly influence riverine resources of the country.  The most important carp seed resources are: eggs, spawn, and fry and fingerlings. 2.1. Egg collection  Large scale egg collection is possible only where locations of the breeding grounds are known and are easily accessible.  Eggs are collected from one or two feet deep water by disturbing the bottom and scooping them with a ‘gamcha’, a rectangular spawn collecting net.  Generally, large scale egg collection is not practiced in rivers. Fry and fingerling collection  The collection of fry (8-40 mm) and fingerlings (40-150 mm) is usually made by cast and drag nets.  Fry and fingerling collection a source of fish seed is prevalent in the Indus river system in Punjab State. Fry (14-25mm) The carp fry can be distinguished from that of catfishes and murrels by the number of dorsal fin rays. a) Major carps: number of undivided dorsal fin rays >11. b) Minor carps: number of undivided dorsal fin rays 11 or <11. c) Catfishes and murrels : Pigmented (either blackish, brownish or orange).
  • 11. Collection of riverine fish seed using a gamcha, a rectangular mosquito netting cloth A haul of fish fingerlings from a riverine stretch (Photo courtesy : Dr Utpal Bhowmick) Fish seed being collected along the bank of a river (Photo courtesy : Dr Utpal Bhowmick) 2.2. Spawn collection Collection of spawn (up to 8 mm) on a commercial scale is prevalent mainly in Bihar, West Bengal and Uttar Pradesh Advantages 1. Traditional and only source of carp seed prior to advent of bundh breeding and hatcheries 2. Collection is cheap and convenient 3. Presently it forms a supplementary source of fish seed
  • 12. 4. Only source for selective breeding programmes Disadvanges 1. Seed consists of both desirable and undesirable species 2. Undependable source 3. Valuable nursery space is wasted by rearing spawn up to fingerlings 4. Mortality of seed during collection and transportation 2.3. Selection of spawn collection site Before selecting a suitable site for the collection spawn in a given stretch of river, a pre-monsoon survey is conducted to ascertain: 1. Topography of the terrain and bank features at the vicinity of the site. 2. Topography of dry bed and bank features. 3. The distribution and composition of fish fauna. 4. The location of tributaries, rivulets and ‘nallahs’ and their confluence with the main river. 5. The identity and accessibility of the site. A river course showing suitable fish spawns collection sites
  • 13. 2.4. Gears used for spawn collection The most commonly used riverine fish spawn collection net is ‘Shooting net’ which is a funnel shaped net of finely woven netting. A typical shooting net (Midnapore type) used to collect riverine fish spawn A battery of shooting nets ready to be commissioned for riverine/brackishwater fish seed collection (Photo courtesy: Dr Utpal Bhowmick) 2.5. Indices of spawn quantity and quality Spawn quantity index: The desirable spawn taken by one standard net during a season is defined as the index of spawn quantity or yield of a centre. Spawn quality index: Total percentage of major carp spawn available in a site. Spawn categories: (i) major carps, (ii) minor carps and (iii) others 2.6. Spawn behaviour in relation to hydrological and hydrobiological characters Flood level: Flood is the most important character showing a positive correlation with spawn availability. Current velocity and water depth: Velocity ranging between 0.5-3 km per hour is conducive to spawn collection. Current direction: A constant direction of current is ideal for spawn collection
  • 14. Weather: Overcast sky, with or without drizzle, coupled with gentle breeze, is generally ideal for spawn collection. Windy weather is unfavourable. Night collections are higher than day collections. Unit 2 - Sexual maturity, breeding season and development of gametes 2.1. Sexual maturity  Maturation of fish gonads (gonadal maturation) is a process by which minute sex cells (germ cells) develop, with accessory tissues, into large organs (testis and ovary) which finally lead to the maturation and release of gametes (sperm and eggs).  Maturation may occur once in the life spawn of a fish or once a year or many times a year, depending on species and conditions.  Age at sexual maturity in fish varies with climatic and ecological /environmental conditions. It varies between species and between sexes. In general, males mature earlier than females.  Size at first sexual maturity also varies with species and between sexes. 2.2. Breeding season  The cultivated carps (with the exception of common carp) attain maturity at the end of second year or the beginning of third year, depending upon the temperature.  They mature during May-June and some are found to remain mature till the end of August.  Majority of the major carps breed during south-west monsoon, in flooded rivers.  The common carp attains maturity at 4-6 months of age and breed year-round in captivity.  Like the common carp, tilapia matures at 4-5 months and spawns through out the year.  Medium carps, catfishes, murrels, etc. mature during pre-monsoon and with the onset of South-West monsoon and breed in ponds, tanks, paddy fields, etc.  Brackish-water and marine fishes attain maturity during pre-monsoon and with the onset of South-West monsoon and have prolonged breeding season.  Gonadotropins regulate the formation and maturation of gametes indirectly through steroid hormones produced by gonad itself.  Maturation has various stages and different sex steroids are involved at each stage, but gonadotropins influence the production of all of them. 2.3. Gamete maturation and release in females Gametes develop and mature in the female fish through a series of stages, before being released. The stages are given below 1. Oogenesis  Oogonia are the cells that give rise to oocytes through a continuopus process called oogenesis and are found throughout the life of a fish.  Oocytes are produced from oogonia as a result of meiosis (reduction division).  Afer the first meiosis the oocytes become surrounded by a layer of epithelial cells called the follicle.  After this the oocyte enters a long stage of cytoplasmic growth.  In fish that breed more than once, oogenesis goes on at various rates throughout the fishes life and is not under the control of hormones (GtH).
  • 15. 2. Primary oocytes growth  The early development of the follicle and its oocyte is independent of pituitary GtH.  The growth is due mainly to proliferation of cellular components.  By the end of this stage, the typical teleost oocyte increases several hundred times in size to a diameter of 100-200µm and is called pre-vitellogenic oocyte.  During the growth period, the follicle cells differentiate to form glandular granulose, separated from oocyte by a zona pellucida and surrounded by an outer theca.  These cells play an important role in steroidogenesis.  The primary growth process continues throughout the life of fish that are multiple spawners and previtellogenic oocytes are present in the ovary year round.  If only the previtellogenic oocytes are present, the ovary is considered immature, which may be became of age or season. 3. Yolk vesicle formation  In response to environmental cues- change in day length, temperature or rainfall, there will be a surge in the GtH levels that induce previtellogenic oocyte to develop further.  The first sign of this stage is the appearance of yolk vesicles in the oocyte cytoplasm.  There contains glycoproteins formed within the oocyte and will eventually become cortical alveoli (may be a source of energy for the embryo) that will be expelled into the previtlline space around the egg after fertilization.  This process is some times referred to as “endogenous vitellogenesis.”  Since there oocytes contain neither true yolk nor vitellogenin, it is better known as “yolk vesicle formation” which is triggered by GtH. 4. Vitellogenesis  Sequestration of the phospholipids, vitellogenin (Vg) from the blood stream and accumulation of true yolk in yolk globules takes place after yolk vesicle formation. This represents the major growth of the oocytes and is known as vitellogenesis.  It involves synthesis of Vg (yolk protein precursor) in the liver, its dlivery to the oocytes via bloodstream and uptake and chemical alterations to form yolk protein.  GtH induces the thecal cells of the follicle to produce testosterone (T), which in turn is converted to an estrogen, 17β -estradiol (17βE2), in the granulosa cells.  E2 travells to the liver in the blood and stimulates production of Vg, which travels to the oocyte, also by the blood stream.  Oocytes sequester Vg as yolk protein in yolk globules and increase in size and this is facilitated by GtH.  The plasma levels of GtH is high during this phase of maturation. T and E2 act on the pituitary in “feed back loops” to regulate GtH release.  Vitellogenesis can be triggered and accelerated by environmental manipulation and hormonal manipulation. 5. Steroid switch and final maturation  Final oocyte maturation in many warm water species is rapid- it usually takes less than 24hrs.  It involves resumption of meiosis, migration of GV (Germinal vesicle) to the edge of the oocyte and GVBD (Germinal vesicle break down), an event that is useful in inducing the oocyte maturity.  Meiosis then stops again and the oocyte is now mature, ready for expulsion from the follicle (ovulation).  During this stage, the level of GtH increases and GtH stimulates the follicle to produce maturation inducing steroid (MIS) instead of estradiol.
  • 16.  MIS is a form of progesterone called 17α - hydroxy progesterone (17α-OHP) or 17α, 20 β- dihydroxy progesterone (7α-20β-diOHP).  At this stage the enzyme responsible for the production of E2 is inhibited. How this steroid switch is regulated is not known.  The MIS induces a number of visible changes in the oocyte during final maturation.  In addition to GV migration and breakdown, it causes an increase in oocyte diameter due to the uptake of water (hydration) in to the cytoplasm and changes in the appearance of the yolk. 6. Ovulation  Final maturation is followed by ovulation- the release of egg from its follicle into the ovarian lumen and is ready to be expelled into the surrounding water for fertilization; ovulation is controlled by prostaglandin (PG) under the influence of GtH.  PG is produced by the follicle and the oviduct; PG are cyclopentane fatty acids.  Once ovulated the eggs of different species remain fertile within the ovary or body cavity for periods of <1 hr to several days.  After this time the eggs become “overripe” and start to disintegrate.  In fish, eggs remain fertile only briefly, (few hours), eg. Carps.  Eggs remain fertile for days, eg. Salmonids  Over-ripening of eggs held in the body after ovulation are highly temperature dependent.  In hormone induced spawning, final maturation and spawning are the most important stages of reproduction. Mature female with orange/yellow ovary 2.4. Gamete maturation and release in males  Fish endocrinologist or breeders are normally biased towards events in the female such as vitellogenesis, final maturation and ovulatory (the three important stages of reproduction). This is because gonadal development in the male goes ahead on its own even in captivity.  In nature, male remains mature over a much longer period than the females and spermatozoa can remain fertile in the testis or outside for much longer periods (up to several days) compared to ovulated eggs which loose fertility very quickly.  However, milt may be scarce and of poor quality, particularly in fishes which need to be induced bred (carps and milk fish) an understanding of sperm formation will be useful in overcoming these practical difficulties.  The testis of fish is sac-like and folded. Like the oocyte, male gametes (sperm cells) develop and mature in the male fish through a series of stages. The stages are given below
  • 17. 1. Spermatogenesis  Spermatogonia are cells that give rise to spermatocytes through a process called Spermatogenesis.  The spermatocytes produced by meiosis (I) are enveloped by sertoli cells until their liberation as mature spermatozoa with in the lumen of the testis.  GtH induces the Leydig cells to produce Testosterone (T), which causes spermatogonia to divide into spermatocytes within cysts of sertoli cells. 2. Spermiogenesis  Development of spermatocytes into spermatids (spermiogenesis) may continue and occurs through 2nd meiosis in the absence of GtH.  The growth of spermatids takes place within the cyst of sertoli cells and at this stage the spermatids possess flagellum. 3. Spermiation  Mature spermatids are liberated into the lumen as spermatozoa through a process called spermiation.  11-ketotestosterone (11-KT), produced by the Leydig cells under GtH stimulation, initiates this process.17α-20β-DiOH P, also produced by the Leydig cells (and possibly by mature spermatozoa), maintains continuous low level spermiation. 4. Hydration  The last step before release of spermatozoa is hydration i. e. dilution of spermatozoa with seminal fluid produced from the walls of the sperm duct under the influence of 17α-20β- DiOH P.  This diluted sperm suspension is called milt which is released during spawning.  Males are considered ‘ripe’ once spermiation starts, although low level spermiation can go on for several months in some species.  Spermiation and sperm hydration increase rapidly when the appropriate environmental cues are present and are often synchronized with final maturation in females.  Males generally are mature even in captivity, if not, environmental/hormonal manipulation can induce spermiation.  Hence males need less hormone than females to induce ‘final maturation’. Mature male with creamy white testis
  • 18. Unit 3 - Breeding of major carps 3.1. Bundhs  Bundhs are special type of tanks or impoundments where riverine conditions are simulated during monsoon months for breeding carps.  They may be perennial (wet bundhs) or seasonal (dry bundhs).  Bundh breeding, which accounted for 5.4% in 1964-65, contributed to about 63% in 1980.  Bundh breeding is popular in Madhya Pradesh and West Bengal.  Bundh breeding seems to have its origin in West Bengal.  Majority of bundh-type of tanks, where major carps are known to breed, are located in the districts of Midnapore and Bankura in West Bengal and around Nowgong in the Chhattarpur district of Madhya Pradesh.  The first dry bundh was located in Sonar Talliya in Nowgong district of Madhya Pradesh.  The initial success achieved by the Department of Fisheries, Government of Madhya Pradesh, during 1958 in dry bundh breeding of carps in Sonar Talliya led to the construction of many more dry bundhs with improved designs.  The most modern constructions are generally masonry structures with arrangements for a sluice gate in the deepest portion of bundh for complete draining and one or two waste weirs for overflow of excess water.  In most cases, apart from the bundh itself, a dry bundh unit consists of storage ponds for stocking breeders, an observation tower with arrangements for storing necessary equipment and a set of cemented hatcheries (2.4m x 1.2m x 0.3m) with a regular supply of water for a large number of eggs at a short time.  In some cases, the embankment is a pucca stone masonry with a small sluice gate and a portion of the embankment itself serves as the waste weir (Dubey, 1969). 3.1.1. Wet bundh A typical wet bundh of Midnapore (West Bengal) is a perennial pond or tank, situated in the slope of a vast catchment area of undulating terrain, with proper embankments having an inlet towards the upland and an outlet towards the opposite lower end.  During summer, only the deeper portion of the bundh retains water where carp breeders are released for spawning.  The remaining portion is dry and is many times used for agricultural purposes.  After a heavy shower, a major portion of the bundh gets submerged with water from catchment area (catchment area: bundh, 20-100: 1), coming into it in the form of streamlets.  The depth of water where breeding takes place has been stated to vary from 8 cm to 1.2 m.  Though the fish breeds at any spot in the bundh, it may be advantageous to prepare `spawning grounds’ at different levels which could get flooded at different water levels in bundhs.  The bundhs can also be filled with water as and when desired and breeding operations undertaken without waiting for the fresh rainwater. Method of breeding carps in a wet bundh  With the onset of monsoon the rain water from the catchment area gushes into the bundh, creating an artificial current.  The breeders, already present in the deeper area of the bundh, migrate to the shallower areas and start breeding.  After the breeding is over, egg/spawn collection is done.
  • 19. 3.1.2. Dry bundh  A typical dry bundh is a shallow, seasonal depression, having a bundh on one side and a catchment area on the other three sides.  The bundh may be of varying shape and size and made of earthen wall or masonry wall.  A dry bundh is smaller and shallower than a wet bundh which is bigger and deeper.  The bundh gets flooded in monsoon, but remains completely dry for a considerable period during a year.  It consists of a sluice gate for quickly draining the water and an outlet for the excess water to flow away. In dry bundhs, spawning sometimes takes place in deeper areas. Technique of breeding major carps in a dry bundh  The mature carp breeders which are raised in perennial ponds elsewhere are introduced into the bundh at 1:2 (Female: Male).  The fish are left undisturbed for 2-3 days so that they get acclimatized to new environment.  After this, 10-20% of the fish is given intramuscular injection of pituitary extract or ready–to- use spawning agents which are synthetic hormones.  Water current is created in the bundh by drawing water from a store tank.  The following morning, the spent breeders are removed, eggs collected, water drained and the bundh dried for 2-3 days.  The bundh is then utilized for the next breeding by releasing a fresh batch of breeders.  Five to six spawning are generally conducted in each bundh during one season as opposed to only one spawning in a wet bundh.  Silver carp and grass carp have been successfully induced bred in bundhs without stripping.  Sinha et al. (1979) have reported natural spawning of both grass carp and silver carp in a dry bundh of Bankura District where they were able to spawn the two species without stripping.  They consider dry bundhs to be one of the reliable means of mass breeding of Chinese carps to meet the increasing demand of their seed.
  • 20. 3.1.3. Collection and hatching of eggs  After spawning is over, the eggs are collected from bundhs, after lowering the water level, by dragging a piece of mosquito netting cloth (gamcha) and released for hatching either in improvised pits or double-walled hatching hapas or cement hatcheries.  The hatching pits (448 cm x244cm x 46 cm) are excavated on the bank with arrangement for the supply of water.  Each pit may contain about 0.9 to 2.2 million eggs, of which 2.5-25% hatch successfully.  A double-walled hapa, which is fixed in the bundh itself, consists of an outer hapa (182 cm x91 cm x 91 cm) and an inner hapa (152 cm x 76 cm x 46 cm ), accounts for a spawn survival rate of 32 to 50%.  The provision of cement hatcheries (2.4 m x 1.2 m x 0.3 m) near the dry bundhs in Madhya Pradesh has aided in improving the survival of hatchlings to 97%.  A cement hatchery of Madhya Pradesh has three times more capacity than a double-walled hapa and is far more economical than the latter. Since the collection of all the eggs is impossible, especially in the case of wet bundhs in view of their larger size, fry and fingerling collection is equally important. 3.1.4. Factors responsible for breeding of fish in bundhs  Spawning may occur at night and during bright sun in the forenoon.  After a period of breeding behaviour, mating occurs with vigorous splashing of water and a number of scales may get dislodged while some fish may even sustain minor injuries.  After spawning is over, a thick blanket of eggs is left behind at the spawning site.  The spent fish in bundhs move to the deeper areas.  No single factors can probably be attributed to spawning of major carps in bundhs and rivers.  The act of spawning involves the completion of a chain of interrelated pre-conditions. 1. Heavy monsoon flood capable of inundating vast shallow areas is believed to be a primary factor responsible for spawning. Some workers believe the availability of shallow spawning ground to be a deciding factor for spawning. The rise in the level of water, naturally or artificially, is known to bring about spawning. 2. The temperature of water for spawning is found to be between 22 and 33°C. 3. Other factors like pH, high Dissolved Oxygen, alkalinity, chloride and minerals do not seem to play any significant role in spawning. Soil type is not very important. 4. Spawning is inhibited due to the presence of hormone-like secretion in captive waters. 5. Water that has flown through a dry bed of land rich in humus has stimulatory effect on spawning. 3.2. Induced breeding of warmwater finfishes and environmental factors affecting spawning  Houssay (1930) of Argentina was the first to attempt induced breeding of fish by using pituitary extract on a viviparous fish. He was successful in obtaining premature birth of young fish.  Subsequently, based on the lines of Houssay, Von Ihering and his team of Brazil, in 1934, successfully induced bred a catfish with pituitary hormones and hence credit for the present day concept of induced breeding of fish goes to Brazilians.  In India, Chaudhuri and Alikunhi (1957) successfully induced major carps to spawn through hypophysation technique.  Since then, the technique has been standardized and refined for the large-scale production of fish seed.
  • 21.  The Indian Major Carp, which normally spawn once a year either naturally or through hypophysation during monsoon, were successfully induced bred twice within an internal of about two months.  Chondar (1984;1990) described a method for the mass scale breeding of IMC and silver carp in `Bangla bundh’ through Human Chorionic Gonadotropin (HCG) and its combination with pituitary extract. A major carp being injected with a spawning agent for induced breeding 3.2.1. Environmental factors concerned with breeding of fishes Environmental factors concerned with fish breeding are  Light  Temperature  Ecological factors  Meteorological conditions These factors are known to play important roles in stimulating the release of pituitary gonadotropins, thereby controlling reproduction in fish. Light  It is an important factor that controls reproduction in fish.  Early maturation and spawning of fish as a result of enhanced photoperiodic regimes.  In India, Cirrhinus reba was found to attain early maturity when subjected to artificial day lengths longer than natural day even at a low temperature of the winter months, viz. 19-20o C.  The resorption of gonads in C. reba was delayed and spawning conditions could be maintained up to November. Temperature  The role of environmental temperature on sexual maturation and spawning of fish in India has been studied.  All observations show that there are optimum temperature ranges for induced breeding of cultivable fishes and critical temperature limits, above and below which fish will not reproduce.  The Indian Major Carps are found to breed within a range of 24-31o C. Beyond this range fish do not spawn.
  • 22.  The Chinese silver and grass carp have been successfully induced bred at temperatures 28.2o C to 34o C.  It was observed natural spawning of pituitary injected grass carp at a water temperature varying between 28.9 and 31.1o C, the optimum being 27o C, as in the case of Indian Major Carps. Other Factors  It was opined that fresh rainwater and flooded condition in a tank are the primary factors in triggering the spawning of carps.  The presence of repressive factors may be responsible for inhibiting spawning of carps in confined waters, but when this repressive factor is sufficiently diluted by the onrush of floods in bundhs or ponds, spawning occurs.  Some workers suggested that it is the sudden drop in the electrolytes level in the environment caused by heavy monsoon rain or water current which induces gonadal hydration, resulting in natural spawning of carps.  Rain water and weather condition are important factors for induced breeding of fish.  Successful spawning in the majority of fishes has been induced on cloudy and rainy days, especially after heavy showers.  The carps are known to breed at a fairly wide range of pH and dissolved oxygen content. 3.2.2. Sympathetic breeding  Sympathetic breeding refers to the breeding of uninjected fish at the sympathy of injected fish.  This is common in bundh breeding, wherein, only 10-20 brooders are injected with either pituitary extract or synthetic spawning agent and the rest are not injected.  After an interval of about 8-10 hours, the injected brooders first start spawning and subsequently the uninjected brooders are also stimulated to spawn, thereby leading to the complete spawning of all the brooders.  Sympathetic spawning leads to lesser use of hormone and reduced handling of brooders.  By this method, natural spawning of both grass carp and silver carp is possible in a dry bundh of Bankura District where they spawned naturally, without stripping.  Some consider sympathetic breeding as one of the reliable means of mass breeding of Chinese carps to meet the increasing demand of their seed. 3.3. Fish Pituitary gland  Pituitary gland is an endocrine (ductless) gland situated on the ventral side of the brain.  It is a small, soft, whitish body whose size and shape vary with species.  It is more or less round in carps; oval in catla and rohu and pear-shaped in mrigal.  The pituitary is located in a concave cavity known as Sella turcica and enclosed by a thin membrane known as duramater.  It may be attached to the brain by a short stalk called the Infundibular stalk. Types of pituitary glands Based on the presence or absence of the stalk, the pituitary is classified into  Leptobasic pituitary (with stalk)– eg. Carps and catfishes  Platybasic pituitary (without stalk)– eg. Murrels and glassfish (Ambasis species) The teleost pituitary comprises of two parts-
  • 23.  The glandular part (the adenohypophysis)  The nervous part (the neurohypophysis) 3.3.1. Collection of pituitary gland Fish pituitary gland can be collected by dissecting and removing a portion of the scalp or through the Foramen magnum. (1) Dissecting and removing a portion of the scalp  In this method, the brain case (cranium) is obliquely cut using a butcher’s knife/hand saw/bone cutter and the scalp removed.  The brain is then exposed by removing grey matter and fatty substance with forceps and cotton.  The anterior end (optic and olfactory nerves) of the brain is cut and the entire brain is lifted up and laid back, thus exposing the pituitary under a membrane.  After removing the membrane and the fluid, the pituitary is lifted up by inserting the blunt end of the forceps and carefully transferred to a vial containing a preservative. Making an oblique cut in the cranium Fatty tissue and grey matter exposed The brain being exposed the pituitary seen as a small whitish body
  • 24. The pituitary mounted on to a wrist (2) Through the Foramen magnum  Foramen magnum is a large posterior aperture of the skull through which the spinal cord passes.  The grey matter and fatty substance are first removed with the help of forceps and cotton (they are pulled out posteriorly).  The brain is then exposed.  After this, the anterior end (optic and olfactory nerves) of the brain is cut and the entire brain is lifted up and laid back, thus exposing the pituitary.  After removing the fluid the membrane, the pituitary is lifted up by inserting the blunt end of the forceps and carefully transferred to a vial containing a preservative. The first method is commonly practiced even though the second method is less time consuming and a large number of glands can be collected within a short time, with a good resale value of the fish. 3.3.2. Preservation of pituitary gland 1. Preservation in absolute alcohol  In this method, the gland, after collection, is immediately transferred to a vial/phial containing fresh absolute alcohol (ethanol).  After 24 hours, the alcohol is removed and fresh alcohol is added and stored at room temperature or in a refrigerator. 2. Preservation in acetone  Immediately after collection, the pituitary gland is kept in ice-chilled acetone and stored in a refrigerator for 2-3 days.  After this period, the acetone is changed and the gland stored in a refrigerator.  Both absolute alcohol and acetone have de-fattening and dehydrating effect. 3. Immediate freezing  In this method, the collected glands are frozen immediately and stored in a freezer. 3.3.3. Preparation of fish pituitary extract for injection  The extract preparation should be carried out just before injection.  The required quantity of glands is taken out of vial and they are dried on a filter paper by allowing the alcohol to evaporate.
  • 25.  The glands are then homogenized with distilled water or saline in a tissue homogenizer.  If acetone-dried glands are used, they can directly be taken for maceration.  One-third of the media is used for homogenization, while the remaining two-third is used for rinsing the homogenizer and the glass rod.  Recommended dilution rate is 20-30 mg in 1 ml of the media.  The extract is centrifuged at 5,000 rpm for 5 minutes.  The clear supernatant solution containing gonadotropins is taken in syringe for injection. Types of injection Homoplastic injection: Injecting pituitary from one fish to another fish closely related to the donor fish. E.g. carp pituitary gland extract to carps. Heteroplastic injection: Injecting pituitary from one fish to another fish distantly related to the donor fish. E.g. carp pituitary gland extract to catfish and vice versa. Methods of injecting fish brooders There are three methods of injecting brooders. They are: 1. Intra-muscular injection:  It is administered into the muscle on the caudal peduncle or behind the dorsal fin, but above the lateral line.  It is most effective, convenient, simple and less risky.  It is widely practiced. 2. Intra-peritoneal injection:  It is give through the soft regions of the body, generally at the base of the pelvic fin or the pectoral fin.  It is risky as it may damage the gonads or liver. 3. Intra-cranial injection:  In this method, the injection is given through the cranium and is also risky as it may damage the brain.  The pituitary extract is administered through a glass or disposable syringe, 2.0 ml capacity, having 0.1 ml graduation.  The size of the needle depends upon the weight of the brooder to be injected.  Needle number 22 is used for fish weighing 1-3 kg, No. 19 for larger fish and No. 24 for smaller fish.  When two injections are given, one is given on the side that did not receive the first injection.
  • 26. 3.3.4. Dosage of pituitary extract  Assessment of proper dosage is most important for successful spawning. In practice, the female receives two injections, while the male receives only one injection, i.e. at the time of second injection to the female.  I Dose or Provocative or preliminary dosage and II Dose or effective or resolving dosage.  The interval between the two doses is 6 hours.  Carp glands to major carps Female Male I Dose 2-3 mg/kg b.w. nil II Dose 5-8 mg/kg b.w. 2-3 mg/kg b.w.  Carp glands to exotic carps Female Male I Dose 4-6 mg/kg b.w. nil II Dose 10-16 mg/kg b.w. 4-6 mg/kg b.w.  Catfish glands to major carps Female Male I Dose 10 mg/kg b.w. nil II Dose 20 mg/kg b.w. 10 mg/kg b.w.  Catfish glands to exotic carps Female Male I Dose 20 mg/kg b.w. nil II Dose 40 mg/kg b.w. 20 mg/kg b.w. 3.4. Synthetic hormones for induced breeding of fishes  Studies conducted by numerous investigators on induced breeding of fishes have indicated the superiority of several ovulating agents over fish pituitary extract.  Although fish pituitary extract was initially used extensively for fish breeding all over the world, synthetic spawning hormones are now being increasingly used due to their efficacy and convenience.  Banerjee et al. (1989) succeeded in the purification of pituitary gonadotropic hormone from Channa punctatus and Catla catla.
  • 27.  Mammalian pituitary hormones in combination with fish pituitary gland extract precipitated spawning in fish.  Of all the mammalian hormones tested on fish, chorionic gonadotropin (CG) has given successful result in inducing fish to breed, probably because CG behaves primarily as a luteinising hormones (LH).  Synahorin (a mixture of CG and mammalian pituitary extract) in combination with pituitary gave positive results when injected to rohu.  Sinha (1969) reported the fractionisation of pituitary extract from carps and tilapia. He obtained success in spawning of carps.  Bhowmick et al. (1979) found mammalian hormones antuitrin-s, leutocyclin and RH-LH ineffective when injected singly or in combination with carp pituitary extract.  The CIFRI, Barrackpore undertook detailed studies on the use of LH-RH alone or in combination with progesterone and obtained breeding success which ranged between 25-49% in carps and 100% in catfish. 3.4.1. Synthetic spawning agents  The stimulation of pituitary gonadotropin secretion by synthetic LH-RH has been demonstrated in a number of teleosts.  Since LH-RH (natural or synthetic) alone is not very effective in inducing spawning in fish, a combination of LH-RH-a (GnRH-a) and a dopamine antagonist for induced ovulation and spawning in cultured fish is a highly effective procedure called the Linpe method.  Some workers reported successful spawning of catla, rohu and mrigal with LH-RH analogue at 10-20 mg/kg b.w. and also obtained 100% ovulation with pimozide at 10mg/kg b.w.  Parameswaran et al. (1988) achieved successful spawning in mrigal with LH-RH-a, buserelin acetate in combination with progesterone.  Investigations of Jose et al. (1989) with LH-RH-a indicated successful breeding of mrigal and Labeo fimbriatus. The Linpe method and ovaprim  Both of these rapidly gained acceptance in fish farms in China and India and has now been commercialized by Syndel Laboratories, Inc., Vancouver, British Columbia, Canada, under the tradename ovaprim.  The ovaprim spawning kit is especially formulated for use with salmonids, cyprinids and other freshwater cultured fish.  It has been used successfully in a number of species in several countries and is gaining wide acceptance as the preferred method for induced ovulation and spawning of cultured freshwater fish.  For example, in India, based on field trials (during 1988-90) with ovaprim for induced spawning of Indian major carps, fringe lipped carp, silver carp, bighead carp and grass carp in various fish farms located in different agro-climatic regions, Nandeesha et al. (1990, 1991) concluded that in economic terms, the use of ovaprim is advantageous.  The spawning success, quantity of eggs obtained, the fertilization rate and hatching percentage remained consistently higher with ovaprim as compared to carp pituitary extract (CPE) or human chorionic gonadotropin (HCG) in almost all instances.  The results also indicate that nearly 40% more fry can be obtained by using ovaprim in place of commercial CPE.  Most of the carps tested generally spawned within 10-14 hours after injection. Ovulation and spawning has been successfully induced in India by the Linpe method in the Asian catfish, Clarias batrachus (Manickam and Joy, !989) and Indian catfish, Heteropneustes fossilis (Manickam, 1992).
  • 28.  Similarly, indigenous preparations, viz. Ovatide (M/s. Hemmopharma Ltd., Mumbai) and WOVA-FH (M/s. WOCKHARDT Ltd., Mumbai) are also being used commonly for the commercial spawning of carps and other fishes in India.  A combination of busereline (LHRH-a) and domperidone has been successfully used for the spawning of IMC (Basavaraja et al., 2007). 3.4.2. Dosage of ready-to-inject spawning agents (ovaprim, ovatide, WOVA-FH, etc.) Females  Catla : 0.4-0.5 ml/kg b.w.  Silver carp : 0.4-0.7 ml/kg b.w.  Rohu : 0.3-0.4 ml/kg b.w.  Grass carp : 0.4-0.8 ml/kg b.w.  Mrigal : 0.25-0.3 ml/kg b.w.  Bighead carp : 0.4-0.5 ml/kg b.w.  Fringe-lipped carp : 0.3-0.4 ml/kg b.w.  Mahseers : 0.6-0.7 ml/kg b.w.  Catfishes : 0.6-0.8 ml/kg b.w. Males (all species of carps) : 0.1-0.3 ml/kg b.w. Males (catfishes) : 0.15-0.4 ml/kg b.w. Steroids  An alternative approach is to use selected steroid hormones targeted at the oocytes. Most of the previous work on this subject is on induced ovulation in vitro.  The effects of steroid hormones on ovulation are seen primarily as germinal vesicle breakdown (GVBD).  GVBD is normally controlled by one or more steroids produced in the ovaries under gonadotropin stimulation, but the timing of ovulation related to that of GVBD varies.  In vitro ovulation normally follows steroid induced GVBD in catfish (H. fossilis). The action of pituitary gonadotropins on final oocyte maturation is known to be medicated through steroid hormones.  Deoxy corticosterone acetate (DOCA) and cortisone effectively stimulated in vitro ovulation in H. fossils.  The available reports indicate that steroid hormones are quite potent in inducing spawning in cultivated fishes, but are yet to find commercial applications.  The thyroid stimulating hormone is also reported to bring about ovulation in Indian catfish.  Although there are no reports on the effects of pheromones on the reproduction of IMC, there are circumstantial evidences which suggest that pheromones secreted by IMC help in effecting spawning.  Similarly, sympathetic spawning of carps in bundhs appears to be due to the release of pheromones. 3.5. Fish broodstock management and transportation of broodfish Management of broodfish ponds  Brood fish is a prerequisite for all induced breeding programmes, as it produces eggs and milt, which are required for the production of larvae.  Proper brood-stock will lead to better breeding responses, increased fecundity, fertilization, hatching and larval survival rates and more viable fish seed.
  • 29.  Hence, the subject of brood fish management has assumed great importance in hatchery management.  The number of brood fish ponds depends on hatchery requirements.  Large-scale of operation and sex-wise segregation of fish requires more ponds. Carp broodfish pond  Carp brood-stock ponds are generally large (0.2-2.5 ha), 1.5-2.5m deep, 30-40m wide, rectangular, seasonal or drainable and earthen in nature.  Water inlet and outlet should be such that they simulate riverine/fluviatile conditions, which is the natural habitat of IMC and Chinese carps. Source of broodfish Since selective breeding and hybridization programmes of pedigreed fish are not carried out in fish seed farms, the source of future broodfish is stock ponds from the same farm or different farms or live adult of different species procured from capture fishery waters like rivers, lakes or reservoirs. Care of broodfish  The carp brood-stock pond should be prepared following standard procedure to ensure sustained production of zooplankton.  The recommended stocking density of carp brood fish is 1,250-2,500 kg/ha, depending upon the species.  While rohu and mrigal are stocked at a higher rate, catla is stocked at a lower rate since it requires more space for proper gonadal development.  Stocking rates are manipulated to permit individual and collective care of broodfish, enabling them to get nutritional and environmental advantages for onset of right degree of maturity.  During immature stage, feed the fish with a traditional diet consisting of rice bran and oil cake (1:1) at a feeding rate of1- 2% of body weight daily.  During the maturing phase, feed the fish with a special feed containing rice bran, oil cake, fish meal, cereals, grams and mineral and vitamin mixture.  Alternatively, one can use commercial floating pelleted feed (protein content : 30%)  In addition to the artificial feed the grass carp is also given tender aquatic weeds/terrestrial grass.  However, the breeding habits of some species like common carp demand their separation from other carp species due to their natural breeding in ponds with aquatic vegetation.  As a result the common carp brood fish is segregated sex-wise and stocked in separate ponds to prevent accidental spawning in pond.  However, the rest of the species can be stocked in a communal pond or stocked in separate ponds after species-wise and/or sex-wise segregation.  Catla, in particular, needs to be separated from the rest of the species as it shows poor response to hormonal injection when stocked with other species.  A gravid fish when held by hand with tail up should practically ooze milt and also ova.  Paddle-wheel aerator, particularly in catla pond, can provide additional aeration, particularly during morning hours.  Segregation of sexes at least one month before increases the affinity between male and female during spawning.  Care should be taken to maintain water quality and plankton level by periodic manuring, i.e. at one tenth of the initial dose.  Algal blooms and oxygen depletion are controlled by water exchange.  Parasites and pathogens should be controlled by periodic checking of brooders  Common parasites like Lernea and Argulus are common on major carps (catla is more susceptible) can be controlled by manually removing and disinfecting the affected fish with a solution of KMnO4 (about 5 ppm)
  • 30. 3.5.1. Broodstock management practice Proper brood fish management forms the key to successful spawning. The number and quality of eggs produced are significantly affected by the conditions under which the brood-stock is maintained.  The quality of brood-stock diet, feeding regime, the quality of brood-stock and water management are the principal factors that influence the condition of the broodstock.  Most seed farms raise broodstock in their own farm (there are instances of inbreeding depression, as reported by Eknath and Doyle (1985) and maintain them in ponds at a density of 1,000-2,500 kg/ha.  The earthen brood-stock ponds vary in area from 0.2 to 1.0 ha, with depth ranging from 1 to 2 m.  The farms use water from perennial reservoirs.  The number of brood fish ponds varies with hatchery requirement.  The main basic steps in the preparation of broodstock ponds are : control of aquatic weeds, which in done manually; eradication of unwanted fish by applying mahua oilcake at 2,000- 2,500 kg/ha and pond liming at 100-200 kg/ha depending on the pH of soil and water.  This is followed by fertilizing the pond with cattle dung, at 15,000-20,000 kg/ha/yr or poultry manure at 5,000-10,000 kg/ha/yr to enhance heterotrophic food production.  In addition, 200-400 kg/ha/yr NPK mixture is applied in split doses at fortnightly or monthly intervals.  The initial dose of organic manure is reduced by half if mahua oil cake is used as piscicide.  After stocking the pond with carps that are one-year-old or more, they are fed with a conventional feed containing a mixture of groundnut oil cake and rice bran (1:1 or 1:2 ratio) at 1-2% b. w., once daily.  To ensure better and timely development of gonads, fish breeders use a special broodstock diet (protein : 25-30%) prepared using locally available cheap ingredients.  This diet is nutritionally superior, advances maturation and spawning by one or two months and results in increased fecundity and better seed quality. Ingredients % Rice bran 25 Groundnut oil cake 25 Fish meal 10 Maize 10 Broken rice 10 Horse gram 10
  • 31. Blackgram 10  This diet is given at 2% b.w. daily, starting in December.  At some farms about a third of the broodstock will be injected with a low dose of HCG at 6-7 mg/kg body weight every 20 days, starting from mid-February for advancing maturation so as to induce spawning by the end of May.  At some seed farms, a few vitamin E tablets are mixed, in addition to a small quantity of commercially available vitamin and mineral mix, in the diet to facilitate gonad development.  Algal blooms and oxygen depletion are the most common problems encountered in broodstock ponds. These can be overcome by frequent water exchange.  Although no major diseases occur in these ponds, infestation by Lernaea and Argulus on catla are common and are controlled by manual removal of adult specimens, followed by a dip treatment in a mild solution of potassium permanganate. 3.5.2. Transportation of broodfish  It is not economically feasible to transport bigger fingerlings/yearlings and broodfish in small packing containers.  For this purpose, truck mounted open tanks with facilitates for mechanical aeration and/or circulation were initially used quite successfully.  Open canvas containers (1m x 1m x 1.25m) are used in Punjab and Madhya Pradesh for transporting major carp breeders. In those States galvanized iron drums of 180 l capacity are also used.  In India, two successful models of closed system of live-fish carrier were designed.  One is due to Mammen (1962), which he called `Splashless tank’.  The later model of the splashless tank is of a petrol tanks design of 1,150 l capacity with an autoclave-type lid.  It has a built-in aeration system for supplying compressed air, which works on a belt driven by the engine of the transporting vehicle.  An oxygen cylinder is carried only as a stand by for emergency.  The inner surface of the tank is lined with U-foam which prevents physical injury to live fish during transport.  A total weight of about 250 kg live fish can be transported at a time in the splashless tank, as also 90,000 carp fingerlings.  The load ratio of fish to water in this type of carrier in about 1 kg of fish per 4.5 l water.  Patro (1968) developed a tank which has an outer chamber of 120 cm diameter open from top and a slightly smaller one closed from top; the latter, during transport, fits inside the former.  The top of the inner chamber is provided with an air vent and an oxygen valve.  The outer chamber serves as a storage tank and is initially filled with water along with fish to be transported.  The inner chamber, which is shipped from the upper open end of the water serves as an oxygen holding chamber at its top and is lined throughout with U-foam to prevent fish from sustaining injury during transport.  This double-barrel type carrier as stated by Patro can transport a total weight of 100 kg of live fish at a time.
  • 32. 3.6. Induced breeding of Indian major carps  Breeding of fish with pituitary gland (hypophysis) extract is termed as Hypophysation  The credit for developing the technique of hypophysation in the world goes to the Brazilians, while the pioneers of hypophysation of Indian major carps are H.L.Chaudhary and K.H.Alikunhi.  Induced breeding refers to inducing fish to release gametes through the application of pituitary extract or hormones or chemicals. Identification of sex of brooders  Identification of sex is a prerequisite to induced spawning of the fish.  Fish is sexually dimorphic and sexual dimorphism is exhibited primarily by gonads and their ducts and this involves killing of fish.  Alternatively, the sex is identified based on certain morphological/external characteristics which include size, length, weight, colouration, fin characteristics, modification in the head in the form of nuptial dress, genital opening, width of mouth, etc.  Carps are sexually dimorphic i.e. mature male and female are morphologically different.  Some of the external morphological characters which are developed during breeding season could be used to identify sex in major carps which mature during their 2nd or 3rd year. Characteristics Male Female 1. Scale, Operculum and pectoral fins Rough to touch, particularly the dorsal surface of pectoral Pectoral smooth to slippery 2. Abdomen Round and firm Swollen and soft 3. Genital opening swollen Elongated slit, white in colour, not swollen Round and pink 4. When pressure applied on abdomen opening milky white fluid oozes through genital opening a few ova may ooze through genital 5. Shape of body and size Body linear, swollen stouter, slightly larger Male (top) and female (bottom) catla brooders 3.6.1. Breeding technique  Induced breeding of carps starts with the onset of south-west monsoon, June.
  • 33.  The male and female brooders are conditioned for a few hours prior to injection.  Sets of brooders are formed, each consisting of 1 : 2 (female : male) ratio.  The injected brooders are released in the breeding hapa. Breeding hapa  A Breeding hapa is a box-shaped cloth enclosure made of long cloth, generally of size 2 x 1 x 1 m with provision to close its top after releasing brooders.  The upper flap is attached to one side and the other sides are either tied or buttoned.  The hapa is fixed in a canal or pond or cement cistern.  The four bottom and four top corners are tied to four poles such that the bottom of the hapa should not touch the ground and one-third of the hapa remain above the water level. Nylon breeding hapas 3.6.1.a. Injection of brooders 1. Intra-muscular injection  It is administered into the muscle on the caudal peduncle or behind the dorsal fin, but above the lateral line.  It is most effective, convenient, simple and less risky.  It is widely practised. 2. Intra-peritoneal injection  It is give through the soft regions of the body, generally at the base of the pelvic fin or the pectoral fin.  It is risky as it may damage the goads or liver. 3. Intra-cranial injection  In this method, the injection is given through the cranium and is also risky as it may damage the brain.  The pituitary extract is administered through a glass or disposable syringe, 2.0 ml capacity, having 0.1 ml graduation.  The size of the needle depends upon the weight of the brooder to be injected.
  • 34.  Needle number 22 is used for fish weighing 1-3 kg, No. 19 for larger fish and No. 24 for smaller fish.  Intra-muscular injection is commonly practiced.  The hormone injection (pituitary/ovaprim/ovatide) is given at the caudal peduncle region in between posterior end of dorsal fin and base of caudal fin, above the lateral line, avoiding the lateral line. Collection of brooders for injection Selection of catla brooder for injection 3.6.1. b. Spawning  After releasing the brooders in the hapa, they should not be disturbed.  After about 6 hours, splashing will commence for breeding and be involved in courtship which will continue for one hour.  At the climax of the courtship, both the partners will be seen in an embrace with their bodies twisted around each other. This exerts pressure on the abdomen, resulting the extrusion of gametes.  The following morning, the spent brooders are removed and then the eggs are collected and transferred for hatching in a suitable hatching device. 3.6.1.c. Examination of eggs After the eggs are water-hardened, a sample of eggs is taken in a beaker for assessing quality and quantity.  The fertilized (good) eggs are transparent with a clearly visible nucleus at the centre and look-like pearls.  The unfertilized (bad) eggs are opaque white and the nucleus disintegrate within one hour.
  • 35. Quantitative assessment of eggs Fertilized eggs circulating in a circular hatchery 3.6.1.d. Fertilization rate It indicates the quality of developing eggs and is estimated using the formula : Fertilization rate (%) = No. of fertilized eggs/Total no. eggs x 100 Hatching rate It can be estimated by knowing the total volume of spawn /number of spawn in a known volume Hatching rate (%) : Total no. of spawn obtained/Total no. of fertilized eggs x 100 3.7. Induced breeding of exotic carps (silver carp and grass carp)  The silver carp and grass carp are native to China and hence are known as exotic carps.  They were first introduced into India in the year 1959.  Both were successfully induced bred in 1963, through hypophysation.  The method of hypophysation of these species is similar to that of IMC.  However, there are a few minor differences – the dosage of pituitary is double compared to IMC and, unlike IMC, they do not spawn naturally after injection, in captivity.  The sex of brooder is identified based on the morphological characteristics such as fins, abdomen, genital opening, etc. The same criteria that are used for IMC can also be used for silver carp and grass carp.  The grass carp can spawn naturally after injection if fed on artificial diet, by avoiding plants, at least two months before spawning.  To facilitate better fertilization, the eggs need to be stripped by applying pressure on the abdomen and eggs fertilized by mixing with similarly stripped milt. Fertilization of eggs  After hormone injection, the male and female brooders are released separately in breeding hapas.  The female brooder is checked for ovulation at 3-4 hours interval.  After ovulation, the female is stripped and the eggs are collected on to a clean basin/tray, avoiding any contamination with excreta, mucus, blood, etc.  Eggs are then mixed with milt stripped from more than one male (preferably 2-3 males) to ensure better fertilization as these fishes produce comparatively less milt.
  • 36.  The water-hardened developing eggs are transferred to incubators for hatching.  The method of hatching of eggs and hatching period are same as that of IMC. Male (top) and female (bottom) grass carp brooders 3.8. Major carp egg and embryonic developmental stages  The fertilization of major carp eggs is external.  The fertilized eggs absorb water and swell considerably in 10-15 minutes and they becomes a little hard and this process is known as water hardening.  They are bead-like, non-adhesive, semi-buoyant and have a large perivitelline space.  The size of the eggs varies between 2.5 and 6.5 mm. Important major carp egg and embryonic stages and time required for each stage are as follows Sl. no. Time required at 24-310 C (Cumulative) Stage 1 0 min Fertilization 2 10-15 min Water hardening and blastodisc formation 3 30-50 min First cleavage (2 cells) 4 40-65 min Second cleavage (4 cells) 5 50-80 min Third cleavage (8 cells) 6 60-90 min Fourth cleavage (16 cells) 7 70-120 min Fifth cleavage (32 cells)
  • 37. 8 2-2½ hrs Morula stage – less than half of yolk invaded by germ layer 9 2½-3 hrs Blastula – about half of yolk invaded by germ layer 10 3-3½ hrs Gastrula – about three-fourth of yolk is invaded by germ layer 11 3-4 hrs Yolk-plug stage – complete invasion of yolk by germ layer excepting a small pore (blastopore) 12 4-6 hrs Elongation of yolk mass-embryonic stage starts 13 5-6 hrs Pea-shaped embryo – somites and myotomes appear 14 8-10 hrs Comma-shaped embryo- optic vesicles appear, occasional twitching movement starts, gill rudiments and pectoral fin bud appear, heart appear 15 14-15 hrs Advanced embryo – increase in the number of somites and myotomes, twitching movement quite rapid 16 18-20 hrs Hatching – it takes place over a period of 2-3 hrs at 24-310 C 3.9. Causes of mortality of fish eggs and spawn and their treatment Improper production and delivery of seed to farmers or poor management of broodfish and fish seed by farmers once stocked, may lead to decline in fish production. Some of the causes of mortality of fish eggs and spawn are:  Poor water quality  Poor pond hygiene  Presence of Pests  Poor management of broodfish and seed  Transportation stress  Conditioning of fish seed  Mortality of eggs and hatchlings  Diseases and parasites Poor water quality  It is known that a lot of major carp eggs perish during incubation as a result of heavy siltation (particularly so during the first few weeks after the onset of monsoon), oxygen depletion, high hydrogen sulfide level, etc.  This problem is all the more serious if reservoir water is taken directly to hatching tank.  Similarly, newly hatched spawn also suffers from the above mentioned problems.  High density of eggs and spawn also contributes to high mortality, particularly in Chinese type of carp hatchery where a large quantity of unfiltered water is used.
  • 38.  Use of filtered water will greatly help reduce mortality of fish eggs and spawn. Poor pond hygiene  Mass mortality of seed in carp nursery ponds is frequently encountered in Karnataka (Mohan and Shankar, 1995).  Mortality of seed is attributed mainly to prevalence of protozoan parasites, particularly in ponds which are not dried properly prior to stocking.  Pond drying followed by liming is known to considerably reduce mortality and improve seed quality. Presence of Pests  The presence of fairly shrimp (Streptocephalus spp) in carp nurseries is known to hamper the growth and survival by competing with fry for food, space and oxygen.  Presence of weed fishes in nursery pond leads to the production of stunted fingerlings. Poor management of broodfish and seed  Competition among fish seed producers to meet demand some times leads to poor management of broodfish and fish seed may negatively affect seed quality.  Substandard quality seed is frequently observed as a result of high stocking density in nurseries.  Fish hatcheries in India are concerned more about the quantity rather than the quality of fish seed and produce them without following any selection norms.  Consequently, the seed suffer from high rates of mortality, poor growth and are prone to diseases and parasites. Transportation stress  During transportation fish seed is subjected to confined environment, higher metabolic load, stress, strain and exhaustion. As a result, the seed becomes susceptible to diseases and parasites.  Chowdhury (1996) used scale loss and tail damage of carp after transportation as quality indicators. Conditioning of fish seed  Conditioning is acclimatizing seed to a restricted environment prior to packing and transportation.  During this period the seed is stocked at a very high stocking density in a hapa or a pond with running water, but without provision for food so that the weak seed dies and only the healthy fry survives.  The seed that survives the `stress test’ only is selected for transport to a required destination. This type of conditioning of fish seed is commonly practiced in several States of India. Mortality of eggs and hatchlings  Fish farmers in West Bengal at times encounter heavy mortality of eggs and hatchlings during incubation period.
  • 39.  This has been found to be due to immature bursting of egg shells and release of premature hatchlings before the anticipated period of hatching. Such hatchlings either do not survive or suffer mortality at subsequent stages.  To overcome this problem, fish farmers of the State have been using a solution which is a mixture of extract of catechu (Acacia catechu) and Myrobalan (Myrobolus indica).  The plant extract enhances hatching period and prevents hatchlings from premature release due to the presence of tannin which helps harden the egg membrane (chorion). Diseases and parasites  High stocking density, artificial feeding, water fertilization, etc. have become common husbandry practices in carp nursery and rearing systems to optimize returns.  These high density systems offer the ideal environment for disease outbreak because such systems have stressed host and virulent pathogen.  Depending on the nature and severity, the disease may cause mass mortality of the affected population in a short time, produce protracted small scale mortality, reduce growth, make the larvae unsuitable for stocking.  The need for adopting suitable health management measures to reduce the loss due to diseases is being increasingly felt by hatchery operators. 3.9.1. Diseases and parasites in carp hatcheries The nursery and rearing systems of carps are often very rich organically and provide an ideal environment for many of the pathogens. Important problems in hatcheries and early rearing systems are caused by some of the following pathogens: 1. Protozoan ciliates like Ichthyophthirius multifilis (white spot disease), Trichodina complex and ectodermal ciliates like Epistylis, Vorticella. All these ectoparasites can cause mass mortality of younger stages of carp very quickly and the situation becomes worst in waters with low oxygen and high organic matter. 2. Disease caused by Myxosporidians are a serious threat to the fish seed farms. These sporozoan spores present in the pond soil are normally ingested by the developing fry. Once inside the target tissue the sporozoans cause massive destruction of their target tissue and produce large scale mortality. 3. Worm parasites like Dactylogyrus (gill fluke) and Gyrodactylus (skin fluke) with their well developed attachment haptor and feeding apparatus can cause mortality in early developmental stages of carps. 4. Opportunistic secondary bacteria (Aeromonas) and fungi (Saprolegnia) can become serious problem in fish larvae which are heavily parasitized. In carp rearing ponds major problems are because of larger ectoparasites, secondary invaders, systemic bacterial pathogens and viruses. Ectoparasites like Dactylogyrus and Argulus (fish lice) and endoparasites like sporozoans are very important. Bacterial problems like surface ulcerative conditions and acute systemic diseases are common in carp rearing systems. Many a times mortalities seen in carp culture systems are a result of ectoparasitic and systemic bacterial diseases.
  • 40. External fungal problems are normally associated with fish which are poorly handled. The possibility of viral diseases causing mortalities in carp nurseries cannot be ruled out. 3.9.2. Bacterial and fungal diseases in carp rearing systems  Bacteria can cause diseases either as secondary invaders or as primary pathogens.  Bacterial diseases in larvae can be broadly classsified as surface ulcerative, acute systemic and chronic granulomatous type.  Surface ulcerative type of diseases are characterized by haemorrhagic surface ulcers and are normally caused by Aeromonas, Pseudomanas, Vibrios, Flexibacteria, Myxobacteria, etc. Surface ulcerative disease conditions at times develop to acute systemic disease.  These are characterized by the presence and proliferation of bacteria in internal organs like kidney, heart, spleen, blood and other visceral organs.  These diseases produce significant necrotic changes in all the affected organs and can cause mortality in a short time scale.  Bacterial haemorrhagic septicaemia caused by numerous serotypes of Aeromonas hydrophila is a major problem. Treatment chart for common disease conditions of carp larval rearing systems Disease agent Chemical Method Concentration/time 1. Ectoparasitic protozoans a. Ichthyophthirius sp. Formalin Short bath 60-100 ppm for 30 minutes b. Trichodina sp. Formalin Long bath 20-30 ppm c. Epistylis & Vorticella Formalin Dip 200-300 ppm for 1 minute d. Ichthyobodos sp. Formalin Dip 20-30 pm for 30 minutes 2. Monogenetic worms a. Dactylogyrus Organophoshporus pesticides Dip 10 ppm for < 1 minute b. Gyrodactylus Nuvan, Dipterex Long bath 0.5 ppm for 24 hours 3. Crustaceans a. Lernaea Nuvan, Dipterex Long bath 0.5 ppm for 24 hours b. Argulus Nuvan, Dipterex Long bath 0.5 ppm for 24 hours 4. Endoparasites a. Sporozoans Nuvan, Dipterex Long bath 0.5 ppm for 24 hours b. Cercaria and metacercaria of digenetic trematodes Nuvan, Dipterex Long bath 0.5 ppm for 24 hours 5. External Mycosis – Saprolegnia Malachite green Formalin Dip Bath Bath 60 ppm for < 1 minute 1-2 ppm for 1 hr 50-75 ppm for 30 minutes 100-200 ppm for 1-3
  • 41. Dip minutes 6. Surface bacterial diseases Proflavine Oxytetracycline & Furnace Bath Short bath 20 ppm 1-5 ppm 7. Systemic bacterial diseases Furazolidone Oxonilic acid Chloramphenicol In feed In feed In feed 50mg /kg fish/day 10mg/kg fish/day 50 mg/kg fish/day Gas disease Heavy mortality of fish seed due to `gas disease’ has been observed on several occasions. The symptoms of the disease are the presence of gas bubbles in gill filaments, heart and blood vessels and also in the gut. The air bladder gets highly distended. The `gas disease’ also referred to as ‘gas embolism’ occurs due to supersaturation of water with either oxygen or nitrogen and is generally encountered in fish nurseries. Unit 4 - Different types of fish hatcheries 4.1. Hatcheries  Presently, hatcheries account for the lion’s share of India’s fish seed production.  The country’s fish seed production which was only 211 million fry in 1964-65, increased to 20,700 million fry in 2008.  A quantum jump in fish seed production from the 1980s is attributed mainly to the introduction of the technology of the Chinese type of carp hatchery and refinement of the technology of induced breeding, coupled with usage of ready-to-use fish spawning agents like ovaprim. However, India still faces shortage of quality fish seed.  West Bengal, where inland fish culture and fish seed production is an age-old practice, continues to be ranked first in fish seed production and supply, far ahead of the 2nd ranked State i.e. Assam, where there is a great demand for freshwater fish.
  • 42. Figure: India’s fish seed production statistics (from 1964-65 to 2003). 4.1.1. Hatchery proper  The evolution of fish (carp) hatchery systems in India was reported by Dwivedi and Zaidi (1983).  Several types of fish hatchery devices are in vogue in India.  These range from the simplest hatching pits to the most modern. 1. Hatching pits  The earliest device of hatching carp eggs, used in bundh-type breeding system of Bengal, comprised pits.  The dimensions of the pits vary according to the requirement, but pits of 3’x 2’x 1’ are dug in multiple rows and their inner walls are plastered with mud.  About 30,000-40,000 eggs can be kept for hatching in each pit. 2. Chittagong type hatchery pits  These are similar to hatching pits except that they are each provided with a double-walled (inner mosquito netting material and outer muslin cloth) cloth linings.  The outer cloth is kept a few inches above the earthen bottom of the pits.
  • 43. 3. Earthen pot hatchery  This is one of the earliest types of hatchery used in association with bundh-type breeding system.  It comprised earthen pots arranged at different levels one draining into the other kept at a lower level.  This provides a flowing current of water, cooled by surface evaporation of the porous earthen pots in which the carp eggs are hatched. 4. Double-walled hatching hapa  The double-walled hatching hapa is one of the commonest devices to serve as an outdoor hatchery.  Installable in a pond or in the margin of a river, up to 1,00,000 eggs can be hatched in the inner mosquito net wall of the hapa of the size 1.75m x 0.90m x 0.75m.  The newly hatched hatchlings wriggle out through the round meshes of the inner wall and collect themselves in the outer whole cloth enclosure  Larval survival is much higher when the hatching hapa is installed in a gently or fast flowing canal or a river because of superior exchange of water. 5. Floating hapa  Similar in principle to the double walled hatching hapa, a floating hapa is mounted on a wooden frame to which it is securely tied such that the whole unit drifts in water.  The floating hapa has the advantage that it can be used on rocky substrates, often found in reservoirs and can be towed to deeper water to obtain better exchange of water. 6. Tub hatchery  First introduced in the Madhya Prudish State.  This hatchery furnishes running water to hatch eggs in galvanized iron units with adjusted levels such that water flows by gravity through interconnecting siphons.  Each tub (2.5’ x 2.5’x 1.5’) is fitted with double walled hapas and the eggs are constantly bathed in a gently flowing water current.
  • 44. 7. Cemented cistern hatchery  Used at Nowgong dry bundh in Madhya Pradesh and is situated generally below the dam sites  Each cement cistern (tank) commonly measures 2.4 x 1.6 x 0.45m.  It has its own inlet, situated at different levels at the opposite ends of the cistern.  They are often arranged in rows wherein water flows by gravity and each cistern can hatch about 3,00,000 eggs at a time. 8. Glass jar hatchery  The credit of developing India’s first transparent hatching device in which the developing eggs can be watched at eye level goes to Bhowmick.  In this system, the hatching are automatically transferred to storage hapa/spawnery, within the hatchery building itself.  The main components of Bhowmick’s glass jar hatchery are : an overhead tank, fish breeding tank, incubation/hatching jars and a spawnery to hold the newly hatched spawn.  The capacity of the overhead tank is 5,500 l and that of each of 20/40 hatching jars is 6.35 l.  The spawnery comprises two cement tanks (1.8 x 0.9 x 0.9 m each) which can hold a nylon hapa measuring 1.65 x 0.8 x 1.0m, projected above the tank and hence deeper than the tank and has an overhead shower for spray.  Each jar can accommodate 50,000 water hardened and swollen eggs at a time. The water flow rate maintained is 600-800 ml/minute for IMC.  This was earlier a very successful system, but the hatchery jars are now obtained against orders only. 9. Transparent polythene jar hatchery  This hatchery is identical to Bhowmick’s glass jar hatchery except that the breakable hatching jars are replaced by transparent polythene containers.  Each jar is provided with an inlet pipe and an outlet pipe and escape of eggs from jars is further prevented by an inner mesquite netting container.  Each jar has its water supply inlet pipe going down almost up to the jar bottom which is of concave type, in hatchery jars.  The water on reaching the jar bottom is reflected upwards till it finds its exit located at the top of the jars.  The water in a jar of this type develops greater churning and suspended eggs therein get better oxygenation.  This is perhaps a superior system than the unidirectional flow pattern of Zoug jars
  • 45. 10. Galvanized iron jar hatchery  This hatchery resembles Bhowmick’s glass jar hatchery except that the hatchery glass jars are replaced by galvanized iron jars.  Each jar has a cylindrical portion (48.5 cm long and 23.0 cm diameter ) and a top conical portion (19 cm long).  The jars are filled on an angle iron framework which also accommodates a galvanized iron conduct (10 cm wide and 10 cm high) to carry the hatchlings from the jars to the spawnery.  The water flow rate is 1 L/min/jar. The galvanized iron jar hatchery is cheaper than glass jar or polythene jar hatchery. 11. Shirgur’s bin hatchery  This hatchery consists of a rectangular aluminum container (54’’ x 16’’ x 22’’) provided with circulating water (243 L/min) in which are placed cylindrical egg vessels (12’’ diameter and 12’’height).  Each egg vessel can hold about 200,000 carp eggs at a time. 12. Hanging dip net hatchery  This hatchery comprises conical 1/16’’ cloth dip nets (65 cm diameter at top and 46 cm diameter at bottom) provided with a 50mm brass spray head at the bottom of each dip net.  Such dip nets are hung in hatching tanks of 3.3 x 1x 1m.  The water flow rate is 1-1.5 l /min during hatching and about 100,000 eggs can be hatched in each container.  After hatching, the hatchlings pass through the meshes of the dip net and get collected in the tank where they are allowed to remain for 3 days before being transferred to nursery pond.  Such units were installed in Orissa State. 13. Circular cistern hatchery  This consists of a galvanized iron circular cistern (tank) of 1 m3 capacity with a series of inlet facets placed at 45o at a height of 5 cm from ground level such that when connected to water supply, water moves in a circular fashion.  The overflowing water is allowed to leave the cistern from an outlet placed at the top by a monofilament 60 mesh/ linear inch.  The hatchlings are left behind in the cistern till their transfer to nursery pond.
  • 46. 14. Chinese type of hatchery  To the Chinese goes the credit of developing the concept of circular breeding and hatching tanks in which water shows circular or centrifugal motion.  Such hatcheries used for hatching carp eggs in India are of Chinese origin and they are called as the Chinese type of carp hatcheries.  This system, within a small space, simulates some aspects of riverine environment and has proved itself a very successful method of breeding carps where commercial production of carp seed is required.  In this system, the outlet lies in the middle of the circular tank guarded by a circular perforated structure or a sloping outlet.  The principle of a hatching tank is similar to that of a breeding tank, excepting that the former is smaller and normally has two chambers, giving the shape of a double doughnut to the hatching tank.  The outlet lies in the middle of the circular tank guarded by a straight, circular perforated pipe which regulates water level in both the chambers.  One wall of the double doughnut lies at the periphery and the other at the inner end surrounding the outlet.  The water circulates between the two walls in a circular fashion, with the help of water jets/ inlets, placed at 45 degree from the bottom.  It is where the eggs are hatched. 15. Low density polyethylene (LDPE) hatchery  S.N. Dwivedi, a former Director of Central Institute of Fisheries Education, Bombay was instrumental in introducing LDPE material in fish hatcheries. This material, like moulded plastic, is made in one piece, has no joints or welds.  This hatchery comprises overhead tanks, cooling tower and compressors. It combines breeding and hatching facilities.  Each vertical hatching jar of capacity 40 L can hold 0.2-0.25 million developing eggs. In earlier days, fish hatchery was used as a facility for hatching of fish eggs collected from rivers and bundhs. At that time, rivers and bundhs were the main sources of carp seed.Over the years the development and refinement of the technique of induced breeding has been enlarged. Hence more emphasis is being given to the hatchery proper for the large scale production of fish fry.
  • 47. Details on the number of Indian major carp hatcheries (both public and private sector), spawn production, conversion rate from spawn to fry, type of hatchery, spawning agent used, etc. are presented below. It is evident that the Chinese type of circular hatchery is the most widely used hatchery for large seed production all over the country. Jar hatchery (glass or fiberglass) and double- walled hatching hapa are used for medium or small scale operation in some parts of the country. Among the different fish spawning agents, ovaprim, a ready-to-use agent, has been the most popular hormone among fish hatchery operators. 4.1.2. Indian major carps (indicative spawn production) State Number of hatcheries Spawn production (lakhs p.a.) Spawn to fry conversion rate Type of hatchery Spawning agent used Andhra Pradesh 20 55,000 30% Jar/circular (Chinese type) Pituitary extract/ ovaprim Arunachala Pradesh 1 n.a. Circular n.a. Assam Circular Pituitary extract/ ovaprim Bihar 4 3,300 <25% Circular Pituitary extract/ ovaprim Gujarath 12 2,200 25% Circular Pituitary extract/ ovaprim Haryana 21 5,150 25% Hapa, circular, jar Pituitary extract/ ovaprim Karnataka 28 6,343 20% Circular, jar, hapa Pituitary extract, ovaprim, HCG, ovatide Kerala 28 21,000 20% Jar, circular, Pituitary extract/ ovaprim Madhya Pradesh 72 15,800 30% Circular,bundhsOvaprim/ovatide Maharastra 28 10,655 30% Circular, hapa Ovaprim/ovatide Manipur 4 160 n.a. Circular Ovaprim, pituitary extract Orissa 37 19,672 30% Circular Pituitary extract/ ovaprim,ovatide Punjab 6 950 30% Circular Ovaprim,ovatide, pituitary extract Rajasthan 19 6,550 n.a. Circular Ovaprim, ovatide, pituitary extract Tamil Nadu 84 8,968 n.a. Hapa, circular Ovaprim, pituitary extract Tripura 5 2,960 Ovaprim, pituitary extract Uttar Prudish 45 11,970 Upto 50% Circular Ovaprim/pituitary extract, HCG
  • 48. West Bengal 30 33,600 Over 30% Circular Ovaprim, ovatide, pituitary extract, HCG Total 420 342,918 Table: Indian major carps (indicative spawn production) n.a.: data not available; data of Assam State not included in the totals; * There are/appears to be more carp hatcheries, particulars of which could not be obtained. Magur, trout and mahseer hatchery details are given in Tables , respectively. However, the seed of these fishes is not produced on a large scale, barring mahseers. 4.1.3. Other fish spawn production Magur (Clarias batrachus) hatcheries state Number of hatchery Spawn production (lakhs p.a.) Spawn to fry conversion rate Type of hatchery Mandhya Prudish 2 1 - - Orissa 1 - - Experimental West Bengal 1 - - Experimental Total 4 1 - - Trout hatcheries State Number of hatchery Spawn production (lakhs p.a.) Spawn to fry conversion rate Arunachal Pradesh 1 n.a. n.a. Jammu and Kashmir 3 6 n.a. Himachal Prudish 4 30 n.a. Tamil Nadu 1 n.a. n.a. Uttar pradesh 3 1.30 n.a. Total 12 37.30 Source : Fishing Chimes, 19 (10 & 11) : 212-213 Mahseer hatcheries State Number of hatcheries Spawn production Spawn to fry conversion Type of hatchery Spawning agent
  • 49. (lakhs p.a.) rate Maharashtra 1 5 n.a. Hatching trays Natural (stripping) Karnataka 1 10 n.a. Hatching trays Ovaprim,ovatide Uttaranchal 2 n.a. n.a. Hatching trays n.a. Jammu and Kashmir 1 10 n.a.. Circular n.a. Kerala 1 n.a n.a. Hatching trays Ovaprim Tamil Nadu 1 Under construction n.a. n.a. n.a. 4.2. Chinese type of carp hatchery  Hatching and breeding devices of circular shape and are of Chinese origin are referred to as Chinese carp hatchery.  A hatchery proper is the most essential component of the modern fish seed farm.  It is here that fish are bred, eggs hatched and hatchlings produced.  Further rearing can even be done outside, but according to the latest concepts of a hatchery, up to fingerling rearing is done in the hatchery or the fish seed farm.  A modern hatchery which incorporates all the essential components and where ecological conditions are simulated is sometimes referred to as eco-hatchery. The components of a hatchery proper are 1. Ante-tank, 2. Fish breeding tanks, 3. Hatching tanks and 4. Larvae holding tank or spawnery.  Ante-tank: The purpose of these tanks is to temporarily hold selected broodfish and to acclimatize them prior to injection and they are normally located inside the hatchery building.  The ante-tanks are normally twins - one each for female and male. A 200 m2 (10m x 20m x 1.5m)ante- tank, divided into two identical twins of 5m x 20m can hold 25 sets of broodfish comprising female to male in the ratio of 1:2 i.e. 75 specimens of fish.  Besides holding broodfish for conditioning, the ante-tanks are also used, in modern hatcheries, for stocking fry or fingerlings prior to sale and also for treatment of diseased or infected fry, fingerling and broodfish. The ante-tank is generally a concrete structure.  Depending upon the system adopted, the same breeding tank can serve as a hatching as well as larval rearing tank for about 3 days after hatching.  The hatching tank can itself serve as a larval rearing tank besides a hatching tank or can in turn lead to a larval rearing tank, where the larvae after yolk-sac absorption are given their first external larval food before being transferred to the outside nursery pond. The merits: The important merits of this system are that it stimulates some characteristics of a riverine habitat where the fish naturally belong, very efficient hatching (almost100%), combines breeding, hatching and larval rearing and suitable for commercial scale operation.
  • 50. The demerits: Requirement of water is high and in many a hatchery water is in short supply and a large breeding tank is ill- utilized for small scale breeding operations. Further, concrete structures are expensive to install and once installed, subsequent modification becomes virtually impossible. 4.2.1. Infrastructure of eco – hatchery complex for carp seed production (Chinese type)  For commercial success of fish hatcheries, infrastructural facilities are prerequisites.  Due to lack of infrastructural facilities, realization of fish seed in nursery/rearing ponds is poor in many states.  Modern carp eco-hatchery is the most appropriate system to produce seed of Indian major carps and exotic carps.  It is an integrated one, with infrastructure for broodfish care, breeding tank, hatching/incubation tank, spawn and fry rearing, packing and marketing of seed, water supply system and buildings. Top view of a Chinese type of circular breeding tank, with an overhead shower A view of a Chinese type of circular hatching tank, with the inner chamber covered with nylon netting (left) and a battery of circular hatching tanks in operation (right). 4.3. Glass jar hatchery  The credit of developing India’s first transparent hatching device in which the developing eggs can be watched at eye level goes to Dr Bhowmick.
  • 51. In this system, the hatching is automatically transferred to storage hapa spawnery, within the hatchery building itself.  The main components of Bhowmick’s glass jar hatchery are : an overhead tank, fish breeding tank, incubation and hatching jars and a spawnery to hold the newly hatched spawn.  The capacity of the overhead tank is 5,000 l and that of each of 20/40 hatching jars is 6.35 l.  The spawnery comprises two cement tanks (1.8 x 0.9 x 0.9 m each) which can hold a nylon hapa measuring 1.65 x 0.8 x 1.0m, projected above the tank and hence deeper than the tank and has an overhead shower for spray.  Each jar can accommodate 50,000 water hardened and swollen eggs at a time. The water flow rate maintained is 600-800 ml/minute.  This was earlier a very successful system, but the hatchery jars are now obtained against orders only. 4.4. Transparent polythene jar hatchery  This hatchery is identical to Bhowmick’s glass jar hatchery except that the breakable hatching jars are replaced by transparent polythene containers.  Each jar is provided with an inlet pipe and an outlet pipe and escape of eggs from jars is further prevented by an inner mosquito netting container.  Each jar has its water supply inlet pipe going down almost up to the jar bottom which is of concave type, in hatchery jars.  The water on reaching the jar bottom is reflected upwards till it finds its exit located at the top of the jars.  The water in a jar of this type develops greater churning and suspended eggs therein get better oxygenation.  This is perhaps a superior system than the unidirectional flow pattern of zoug jars. Indoor transparent (left) and outdoor fiberglass jars (right) used for hatching major carp eggs 4.5. Galvanized iron jar hatchery
  • 52.  This hatchery resembles Bhowmick’s glass jar hatchery except that the hatchery glass jars are replaced by galvanized iron jars.  Each jar has a cylindrical portion (48.5 cm long and 23.0 cm diameter ) and a top conical portion (19 cm long).  The jars are filled on an angle iron framework which also accommodates a galvanized iron conduct (10 cm wide and 10 cm high) to carry the hatchlings from the jars to the spawnery.  The water flow rate is 1 L/min.  The galvanized iron jar hatchery is cheaper than glass jar or polythene jar hatchery. Unit 5 - Fish seed rearing techniques 5.1. Fish spawn rearing techniques – nursery pond In India, carp culture is carried out in three phases (three-tier system) comprising: 1. Nursery phase (rearing three-day-old spawn to fry), 2. Rearing phase (rearing fry to fingerling stage) and 3. Grow out phase (rearing fingerlings to adult stage). Of the three phases the nursery rearing is crucial and needs greatest attention of fish hatchery managers.  Jhingran and Pullin (1985, 1988) have reviewed the subject of nursery and rearing pond management. The allocation of land for different types of ponds and other purposes is shown below : under Indian conditions a 4 ha farm should be divided into nursery ponds, 0.2 ha; rearing ponds, 0.8 ha and stocking ponds, 3 ha.  Considering the enhanced rate of stocking and survival of spawn, fry and fingerlings, the ratio of nursery to rearing to stock ponds should be 1:40:1280.  The preparation of nursery, rearing and stocking ponds before releasing the stocking material is an important step for successful rearing of carp spawn to fry, fry to fingerlings and fingerlings to table-sized fish.  The occurrence of large scale mortality of stocked spawn in unprepared nurseries is a common experience of fish culturists throughout the country.  Basavaraja and Joseph Antony (1997) obtained survival as high as 100% in the IMC nursery.