Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free ... more Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free and immobilized brewing yeast fermentation performance based on kinetic parameters. Bulg. J. Agric. Sci., 26 (4), 899–905 The study was focused on comparing two different immobilized brewing yeast Saccharomyces carlsbergensis fermentations with traditional free cell fermentation in experimental scale. The immobilization techniques used were: entrapment and capsulation method in alginate support. The objective was to choose the most suitable immobilization technique that protects the yeast cells providing a good fermentation performance compared to free yeast cell fermentation. Kinetic parameters investigation of free and immobilized Saccharomyces carlsbergensis was done based on growth kinetics, ethanol productivity and substrate consumption (glucose) using computer simulation for different kinetic models. Entrapment and capsulation immobilization techniques are applicable, effective and...
In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tom... more In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tomato pulp was investigated. Also, the kinetics of the extraction of carotenoids from tomato pulp, using as solvent the liquid carbon dioxide under its liquid-vapor equilibrium conditions was study. The experiments were carried out in a Jennings-type autoclave after the Soxhlet principle with and without modifier at 299 K and 64 bar. The extraction yields and the lycopene and β-carotene content of the liquid CO 2 extracts were determined after 0.5, 1, 3, every three hours, up to 39 hours of extraction in the presence of oleic acid as modifier. HPLC-DAD was used for the quantification of the lycopene and β-carotene in the extracts. The highest amount of trans-lycopene is extracted after 12 hours of extraction (0.26 μg/g sample) and β-carotene (0.33 μg/g sample). Only after 12 hours of extraction the amount of lycopene and β-carotene extracted from tomato pulp in presence of oleic acid, beco...
Background: Sideritis scardica and S. raeseri are very popular medicinal plants in Albania, Maced... more Background: Sideritis scardica and S. raeseri are very popular medicinal plants in Albania, Macedonia, and Bulgaria. Objective: Comparison of the phenolic, flavonoid, and diterpene contents and detailed chemical profiles, as well as data on antioxidant activity, comprising plant material from different locations in the Balkan Peninsula. Materials and Methods: Total phenolic and flavonoid contents for the studied Sideritis samples were determined spectrophotometrically. By HPLC-DAD-ESI-MSn analysis, 48 individual phenolic constituents were completely or tentatively identified. Chemical profiling of Sideritis major diterpenes and their quantification was performed by HPLC-ESI-MSD TOF analysis. A combination of two methods (ORAC and DPPH) was applied for evaluation of the antioxidant capacity of Sideritis extracts. Results: The results obtained demonstrated that Sideritis samples (S. scardica and S. raeseri) collected from Albania, Bulgaria and Macedonia are similar in their chemical p...
The idea of immobilization or known also as microencapsulation was first introduced in 1964 and t... more The idea of immobilization or known also as microencapsulation was first introduced in 1964 and the aim was transplanted cells protection. Cell immobilization in alcoholic fermentation is a rapidly expanding research area because of its attractive technical and economic advantages compared to the conventional free cell system. In this study we analyze two different immobilization techniques of beer and bread yeast in alginate beads. Yeast was immobilized with two different methods of immobilization, entrapment and capsulation in alginate and inoculated in two different mediums. Objective was to compare immobilized fermentation rate related to free yeast cells process and how the medium influence the fermentation process. Mediums used were beer wort and sugar solution. Comparison was made in terms of substrate consumption rate, fermentation kinetic coefficients and optimum fermentation medium. A continuous fermentation process was developed. There were no notable differences between ...
Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free ... more Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free and immobilized brewing yeast fermentation performance based on kinetic parameters. Bulg. J. Agric. Sci., 26 (4), 899–905 The study was focused on comparing two different immobilized brewing yeast Saccharomyces carlsbergensis fermentations with traditional free cell fermentation in experimental scale. The immobilization techniques used were: entrapment and capsulation method in alginate support. The objective was to choose the most suitable immobilization technique that protects the yeast cells providing a good fermentation performance compared to free yeast cell fermentation. Kinetic parameters investigation of free and immobilized Saccharomyces carlsbergensis was done based on growth kinetics, ethanol productivity and substrate consumption (glucose) using computer simulation for different kinetic models. Entrapment and capsulation immobilization techniques are applicable, effective and...
In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tom... more In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tomato pulp was investigated. Also, the kinetics of the extraction of carotenoids from tomato pulp, using as solvent the liquid carbon dioxide under its liquid-vapor equilibrium conditions was study. The experiments were carried out in a Jennings-type autoclave after the Soxhlet principle with and without modifier at 299 K and 64 bar. The extraction yields and the lycopene and β-carotene content of the liquid CO2 extracts were determined after 0.5, 1, 3, every three hours, up to 39 hours of extraction in the presence of oleic acid as modifier. HPLC-DAD was used for the quantification of the lycopene and β-carotene in the extracts. The highest amount of trans-lycopene is extracted after 12 hours of extraction (0.26 μg/g sample) and β-carotene (0.33 μg/g sample). Only after 12 hours of extraction the amount of lycopene and β-carotene extracted from tomato pulp in presence of oleic acid, becom...
Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free ... more Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free and immobilized brewing yeast fermentation performance based on kinetic parameters. Bulg. J. Agric. Sci., 26 (4), 899–905 The study was focused on comparing two different immobilized brewing yeast Saccharomyces carlsbergensis fermentations with traditional free cell fermentation in experimental scale. The immobilization techniques used were: entrapment and capsulation method in alginate support. The objective was to choose the most suitable immobilization technique that protects the yeast cells providing a good fermentation performance compared to free yeast cell fermentation. Kinetic parameters investigation of free and immobilized Saccharomyces carlsbergensis was done based on growth kinetics, ethanol productivity and substrate consumption (glucose) using computer simulation for different kinetic models. Entrapment and capsulation immobilization techniques are applicable, effective and...
In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tom... more In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tomato pulp was investigated. Also, the kinetics of the extraction of carotenoids from tomato pulp, using as solvent the liquid carbon dioxide under its liquid-vapor equilibrium conditions was study. The experiments were carried out in a Jennings-type autoclave after the Soxhlet principle with and without modifier at 299 K and 64 bar. The extraction yields and the lycopene and β-carotene content of the liquid CO 2 extracts were determined after 0.5, 1, 3, every three hours, up to 39 hours of extraction in the presence of oleic acid as modifier. HPLC-DAD was used for the quantification of the lycopene and β-carotene in the extracts. The highest amount of trans-lycopene is extracted after 12 hours of extraction (0.26 μg/g sample) and β-carotene (0.33 μg/g sample). Only after 12 hours of extraction the amount of lycopene and β-carotene extracted from tomato pulp in presence of oleic acid, beco...
Background: Sideritis scardica and S. raeseri are very popular medicinal plants in Albania, Maced... more Background: Sideritis scardica and S. raeseri are very popular medicinal plants in Albania, Macedonia, and Bulgaria. Objective: Comparison of the phenolic, flavonoid, and diterpene contents and detailed chemical profiles, as well as data on antioxidant activity, comprising plant material from different locations in the Balkan Peninsula. Materials and Methods: Total phenolic and flavonoid contents for the studied Sideritis samples were determined spectrophotometrically. By HPLC-DAD-ESI-MSn analysis, 48 individual phenolic constituents were completely or tentatively identified. Chemical profiling of Sideritis major diterpenes and their quantification was performed by HPLC-ESI-MSD TOF analysis. A combination of two methods (ORAC and DPPH) was applied for evaluation of the antioxidant capacity of Sideritis extracts. Results: The results obtained demonstrated that Sideritis samples (S. scardica and S. raeseri) collected from Albania, Bulgaria and Macedonia are similar in their chemical p...
The idea of immobilization or known also as microencapsulation was first introduced in 1964 and t... more The idea of immobilization or known also as microencapsulation was first introduced in 1964 and the aim was transplanted cells protection. Cell immobilization in alcoholic fermentation is a rapidly expanding research area because of its attractive technical and economic advantages compared to the conventional free cell system. In this study we analyze two different immobilization techniques of beer and bread yeast in alginate beads. Yeast was immobilized with two different methods of immobilization, entrapment and capsulation in alginate and inoculated in two different mediums. Objective was to compare immobilized fermentation rate related to free yeast cells process and how the medium influence the fermentation process. Mediums used were beer wort and sugar solution. Comparison was made in terms of substrate consumption rate, fermentation kinetic coefficients and optimum fermentation medium. A continuous fermentation process was developed. There were no notable differences between ...
Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free ... more Pinguli, L., Troja, R., Malollari, I., Gurazi, V. & Vaso, T. (2020). A comparative study of free and immobilized brewing yeast fermentation performance based on kinetic parameters. Bulg. J. Agric. Sci., 26 (4), 899–905 The study was focused on comparing two different immobilized brewing yeast Saccharomyces carlsbergensis fermentations with traditional free cell fermentation in experimental scale. The immobilization techniques used were: entrapment and capsulation method in alginate support. The objective was to choose the most suitable immobilization technique that protects the yeast cells providing a good fermentation performance compared to free yeast cell fermentation. Kinetic parameters investigation of free and immobilized Saccharomyces carlsbergensis was done based on growth kinetics, ethanol productivity and substrate consumption (glucose) using computer simulation for different kinetic models. Entrapment and capsulation immobilization techniques are applicable, effective and...
In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tom... more In this study, the effect of oleic acid during the extraction of lycopene and β-carotene from tomato pulp was investigated. Also, the kinetics of the extraction of carotenoids from tomato pulp, using as solvent the liquid carbon dioxide under its liquid-vapor equilibrium conditions was study. The experiments were carried out in a Jennings-type autoclave after the Soxhlet principle with and without modifier at 299 K and 64 bar. The extraction yields and the lycopene and β-carotene content of the liquid CO2 extracts were determined after 0.5, 1, 3, every three hours, up to 39 hours of extraction in the presence of oleic acid as modifier. HPLC-DAD was used for the quantification of the lycopene and β-carotene in the extracts. The highest amount of trans-lycopene is extracted after 12 hours of extraction (0.26 μg/g sample) and β-carotene (0.33 μg/g sample). Only after 12 hours of extraction the amount of lycopene and β-carotene extracted from tomato pulp in presence of oleic acid, becom...
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