mansi kumari

The introduction of spacers in coating steroid protein complex and / or enzyme conjugates or immunogen is known to exert an influence on the sensitivity of steroid enzyme immunoassays. We have introduced different homobifunctional spacers between enzyme and prednisolone (PSL) moiety having atomic length 3 to 10 and studied their influence on functional parameters such as sensitivity and specificity of PSL enzyme immunoassays. In this study, four enzyme conjugates of PSL-21-HS and HRP were prepared by N-Hydroxy Succinimide mediated carbodiimide reaction, these were PSL-21-HS-adipic acid dihydrazide (ADH)-HRP, PSL-21-HS- carbohydrazide (CH)-HRP, PSL-21-HS-ehylenediamine (EDA)-HRP and PSL-21-HS- urea (U)-HRP. The assays were developed using these enzymes conjugate with antibody raised against PSL-21-HS-BSA immunogen. The sensitivity of the PSL assays after insertion of bridge in enzyme conjugate were 1.22 ng/mL, 0.59 ng/mL, 0.48 ng/mL and 0.018 ng/mL with ADH, CH, EDA and urea as space...

The study is focused on luxury brands and their marketing strategy inclusive of SOPs and Social media marketing. Luxury brand s are famous for its uniqueness and exclusiveness. What makes them demanding and irresistible to customers is their strategy of scarcity of products, creating an image of them being extremely luxurious and status centric. The study shows how the luxury brands market themselves with the help of S EO and Social Media marketing. The products being very exclusive in nature it is very difficult for the brand to find a customer base and sell customized pr oducts.At the end of this study we will be able to know how various luxury brands market their products and make them visible to th eir prospective customers through Search Engine Optimization and Social Media Marketing.