Shaharior Hossen
Chonnam National University, Department of Fisheries Science, Graduate Student
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Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (Hdh-CAT) in stress... more
Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (Hdh-CAT) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. Hdh-CAT gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of Hdh-CAT had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence in situ hybridization confirmed Hdh-CAT localization in the digestive tubules of the DG. Hdh-CAT was induced by different types of stress including thermal stress, H2O2 induction, and starvation. Immune challenges with Vibrio, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated Hdh-CAT mRNA expression and catalase activity. Hdh-CAT responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated...
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Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of TPM in Haliotis discus hannai (Hdh-TPM) was 2160 bp, encoding 284 amino acids, and contained a TPM... more
Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of TPM in Haliotis discus hannai (Hdh-TPM) was 2160 bp, encoding 284 amino acids, and contained a TPM signature motif and a TPM domain. Gene ontology (GO) analysis based on the amino acid sequence predicted Hdh-TPM to have an actin-binding function in the cytoskeleton. The 3D analysis predicted the Hdh-TPM to have a coiled-coil α-helical structure. Phylogenetically, Hdh-TPM formed a cluster with other TPM/TPM1 proteins during analysis. The tissue-specific mRNA expression analysis found the higher expression of Hdh-TPM in the heart and muscles; however, during embryonic and larval development (ELD), the higher expression was found in the trochophore larvae and veliger larvae. Hdh-TPM expression was upregulated in fast-growing abalone. Increasing thermal stress over a long period decreased Hdh-TPM expression. Long-term starvation (>1 week) reduced ...
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FMRFamide-related peptides are neuropeptides involved in a wide range of biological processes, including reproduction and larval development. To characterize the involvement of FMRFamide in the reproduction and larval development of... more
FMRFamide-related peptides are neuropeptides involved in a wide range of biological processes, including reproduction and larval development. To characterize the involvement of FMRFamide in the reproduction and larval development of Pacific abalone Haliotis discus hannai, an FMRFamide cDNA (Hdh-FMRF2) was cloned from the cerebral ganglion (CG). Fluorescence in situ hybridization and qRT-PCR were performed for functional characterization. The Hdh-FMRF2 cDNA encoded 204 deduced amino acids that contained a putative signal peptide and four FaRP domains. The major population of Hdh-FMRF2 neuronal cell bodies was localized in the cortex of CG. Hdh-FMRF2 mRNA expression was significantly upregulated in CG during the mature stage of gonadal development and effective accumulative temperature (EAT) exposed abalone in both sexes. In the induced spawning event, Hdh-FMRF2 expression was significantly upregulated during spawning in males. However, no upregulation was observed in females, suggest...
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Fish reproduction is regulated by the brain–pituitary–gonad (BPG) axis where the gonadotropin-releasing hormone (GnRH) plays a central role. Seed production of small yellow croaker (Larimichthys polyactis) is performed using... more
Fish reproduction is regulated by the brain–pituitary–gonad (BPG) axis where the gonadotropin-releasing hormone (GnRH) plays a central role. Seed production of small yellow croaker (Larimichthys polyactis) is performed using captive-reared broodstock known to undergo reproductive dysfunction, which is connected to endocrinological dysfunction. To determine the endocrinological mechanism of GnRHs in the BPG axis of small yellow croaker, full-length sequences of three GnRH isoforms encoding sbGnRH (GnRH1), cGnRH-II (GnRH2), and sGnRH (GnRH3) were cloned and characterized from brain tissue. qRT-PCR, in vivo, and in vitro experiments were performed for functional characterization. The mRNA expression of GnRH1 in the brain and gonadotropin subunits (GPα, FSHβ, and LHβ) in the pituitary were significantly higher at the ripen stage during gonadal development and GnRH1 at spawning stage during spawning events. Expression of both GnRH1 and GtH subunits was significantly lower in females than...
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The Pacific abalone Haliotis discus hannai is a highly commercialized seafood in Southeast Asia. The aim of the present study was to determine the antioxidant activity and oxidative stress-oriented apoptosis pathway in saccharides... more
The Pacific abalone Haliotis discus hannai is a highly commercialized seafood in Southeast Asia. The aim of the present study was to determine the antioxidant activity and oxidative stress-oriented apoptosis pathway in saccharides supplemented cryopreserved sperm of Pacific abalone. Cryopreserved sperm showed impaired antioxidant defenses due to the reduced mRNA abundance of antioxidant genes (CAT, Cu/Zn-SOD, Mn-SOD, GPx, GR, and BCL-2), apoptosis inhibitor (HSP70, and HSP90) gene, and enzymatic antioxidant activity compared to fresh sperm. Such impaired antioxidant defenses caused an increase in the mRNA expression of apoptosis genes (Bax, and Caspase-3), finally leading to apoptosis. The impaired antioxidant defense also increased O2•− production and lipid peroxidation (MDA) levels, which further accelerated apoptosis. Considering all the experimental findings, an apoptosis pathway of cryopreserved sperm has been adopted for the first time. Specifically, sperm cryopreserved using ...
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As structural components of sperm, tektins are thought to play a fundamental role in sperm flagellar motility. In this study, Tektin-4 (Hdh-TEKT4) gene was successfully cloned and characterized from the testis tissue in Pacific abalone,... more
As structural components of sperm, tektins are thought to play a fundamental role in sperm flagellar motility. In this study, Tektin-4 (Hdh-TEKT4) gene was successfully cloned and characterized from the testis tissue in Pacific abalone, Haliotis discus hannai. The full-length cDNA of Hdh-TEKT4 was 1,983 bp, with a coding region of 1,350 bp encoding 51.83 kDa putative protein of 449 deduced amino acids. Hdh-TEKT4 contains a tektin domain including a nonapeptide signature motif (RPGVDLCRD). Fluorescence in situ hybridization revealed that Hdh-TEKT4 localized in the spermatids of Pacific abalone testis. qRT-PCR analysis showed that Hdh-TEKT4 was predominantly expressed in testis tissues. Hdh-TEKT4 mRNA expression was upregulated during the fully mature testicular developmental stage in both seasonal development and EAT exposed abalone. Furthermore, mRNA expression of Hdh-TEKT4 was significantly higher in sperm with higher motility than in sperm with lower motility during peak breeding ...
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As a multinational river, the Naf River flows into the Bay of Bengal in the Indian Ocean, between the Cox's Bazar district of Bangladesh and the Rakhine state of Burma. In a multidisciplinary approach, several experiments were carried... more
As a multinational river, the Naf River flows into the Bay of Bengal in the Indian Ocean, between the Cox's Bazar district of Bangladesh and the Rakhine state of Burma. In a multidisciplinary approach, several experiments were carried out to understand the seasonal diversity of the phytoplankton community structure. A total of four layers of water was sampled from four depths in the Naf River during monsoon (September) and winter (December) of 2016. 41 species of phytoplankton were identified, and 3 different dominant groups (Cyanobacteria, Diatoms, and Dinoflagellates) were found. Diatoms and cyanobacteria alone were found to be most prevalent. Higher species diversity was observed in the monsoon season, with Synedra sp. (1.84×105 cells L-1, 18.76%) and winter with Microcystis sp. (1.41×105 cells L-1, 17.74%), respectively. In monsoon, NO3-N and PO4-P were both higher than winter (450.9 and 34.4 µg L-1, respectively) especially, at downstream Naf River. Moreover, high diversity...
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The present investigation was conducted to assess the status of fish farming and livelihood of fish farmer in the Barisal Sadar Upazila, Southern Bangladesh for a period of ten months from March 2015 to January 2016. Well-structured... more
The present investigation was conducted to assess the status of fish farming and livelihood of fish farmer in the Barisal Sadar Upazila, Southern Bangladesh for a period of ten months from March 2015 to January 2016. Well-structured questionnaire was applied for primary data collection from fish farmer. The survey revealed that average pond size was 0.12 ha with 85% of the farmers having ponds of single ownership. Majority farmer (94%) preferred poly-culture system where maximum pond was seasonal (84%). Most of the fish farmers were belonged to the age groups of 36 to 50 years (52%), represented by 80% Muslims.Majority farmer (68%) had tin-shed house where majority were taken health facilities from Upazila doctor (32%). Most of the farmer (55%) lived in joint families where majority family members (41%) were between 4 -5. About 7% had no education while 11%, 49%, 21% and 12% had primary, secondary, higher secondary and bachelor level of education respectively. Fish farming was main ...
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This study was carried out to explore the fish marketing system and species combination in five village fish markets of Paikgachha Upazila, Bangladesh. Nine marketing channels were found in the retail markets and the shortest channel was... more
This study was carried out to explore the fish marketing system and species combination in five village fish markets of Paikgachha Upazila, Bangladesh. Nine marketing channels were found in the retail markets and the shortest channel was observed when consumers directly bought fish from the producers. Most of the retailers were 30-45 years old whereas, 40% had no opportunity to receive institutional education though all of them can sign their name. In the retail markets, most of the fishes were sold without ice. Moreover, a total of 93 species were found whereas 65.59, 10.75, 9.68 and 13.98% were inland fish, marine fish, exotic fish and crustacean species, respectively. Perciformes (29.03%) outnumbered the other 11 orders and Cyprinidae was represented as dominant following 38 families. Most of the fish and shellfishes were (73.12%) available from capture fisheries and the fishes were harvested from nearby rivers of the study area. In the markets 37, 10, 8 and 6 inland species were in the least concern, near threatened, endangered and vulnerable category, respectively in the aspect of their biodiversity status in Bangladesh. Among the inland fishes, 39 species were small indigenous fish species (SIS) and demands for riverine fishes were higher than the culture fishes. The price of most of the fish and shellfishes were within 251 to 500 BDT per kg whereas, the maximum price of Tenualosa ilisha was higher than 750 BDT kg. Thirteen major constraints in the fish markets were identified by the fish traders.
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The present investigation was conducted to assess the status of fish farming and livelihood of fish farmer in the Barisal Sadar Upazila, Southern Bangladesh for a period of ten months from March 2015 to January 2016. Well-structured... more
The present investigation was conducted to assess the status of fish farming and livelihood of fish farmer in the Barisal Sadar Upazila, Southern Bangladesh for a period of ten months from March 2015 to January 2016. Well-structured questionnaire was applied for primary data collection from fish farmer. The survey revealed that average pond size was 0.12 ha with 85% of the farmers having ponds of single ownership. Majority farmer (94%) preferred poly-culture system where maximum pond was seasonal (84%). Most of the fish farmers were belonged to the age groups of 36 to 50 years (52%), represented by 80% Muslims.Majority farmer (68%) had tin-shed house where majority were taken health facilities from Upazila doctor (32%). Most of the farmer (55%) lived in joint families where majority family members (41%) were between 4-5. About 7% had no education while 11%, 49%, 21% and 12% had primary, secondary, higher secondary and bachelor level of education respectively. Fish farming was main occupation of 24% farmer where majority (30%) was involved with agriculture. Majority farmer (74%) had semi-pucca toilet where 16% had own tube-well. 53% farmer were used wood as cooking fuel where 74% of farmers used their own money for fish farming. 37% were capable to save money in bank or different types of NGOs where 95% farmers improver their socioeconomic conditions by fish farming. Majority fish farmer (84%) achieved training on fish farming from NGOs where 9% had not received any training. Majority households (94%) had electricity facility where most of the farmer (70%) had ordinary social status. Seasonal flood (26%), non-availability of fish fry (25%), insufficient water in dry season (12%), lack of money (11%), Fish disease (9%), pouching (8%), lack of quality feed (5%) and poor technical knowledge (4%) for fish farming was identified as the major constraints.
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The investigation was conducted to evaluate the socioeconomic profile of the fishermen, using Best-Worst scale and Pearson’s correlation of Sugondha River, Bangladesh during the period of July 2015 to June 2016 by interviewing 200... more
The investigation was conducted to evaluate the socioeconomic profile of the fishermen, using Best-Worst scale and Pearson’s correlation of Sugondha River, Bangladesh during the period of July 2015 to June 2016 by interviewing 200 fishermen. In Sugondha River, 47% fishermen were engaged in fulltime fishing and 56% preferred group fishing. Maximum fishermen (49%) were middle aged and 56% were found Illiterate. 58% fishermen were lived with joint family and 17% fishermen had 8-above family members. Average annual income of most of the fishermen ranged from was BDT 56,000-70,000 (32%), where 30% had less than BDT 41,000. 48% women of the fishermen family supported their family by Poultry rearing. Age of fishers and income was positively correlated (r = 0.071) where income showed positive relationship with saving (r = 0.765; p<0.01), type (r = 0.561; p<0.01), fishing (r = 0.759; p<0.01), housing (r = 0.351; p<0.01), sanitation (r = 0.540; p<0.01) and health (r = 0.535; p&...
The post stocking control mechanism of pond fish culture in Abyaynagar, Manirampur, Bagherpara and Chaugacha Upazila under Jessore district was observed from May to November 2016 by interviewing 200 fish farmers. It reflects, majority... more
The post stocking control mechanism of pond fish culture in Abyaynagar, Manirampur, Bagherpara and Chaugacha Upazila under Jessore district was observed from May to November 2016 by interviewing 200 fish farmers. It reflects, majority farmer (56%) cultured tilapia fish but only 3% cultured Shrimp/Prawn. The highest stocking density (63%) was found 100 to 200 fry/decimal having 0.5 to 2.0 cm (72%) fingerling size with directly grow out pond stocking (52%). About 58% framer used balance feed with broadcasting method in the morning feeding (77%) only. Most of the farmers did not exchange water (58%) and can’t culture properly (66%) due to lack of money. Only 7% farmers went to the government fisheries officials where 40% went to chemical shopkeepers for getting tips if any problems took place. It’s therefore, important to offer with institutional and organizational helps, credit and extension services for sustainable fish production in the Southwestern Bangladesh.
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The Sundarbans form a perfect mangrove ecosystem, which supports a large group of fish, shrimp, edible crab and also supply food and cash to local coastal communities. The present study was conducted to evaluate the livelihood status of... more
The Sundarbans form a perfect mangrove ecosystem, which supports a large group of fish, shrimp, edible crab and also supply food and cash to local coastal communities. The present study was conducted to evaluate the livelihood status of the fishermen Engaged in Fishing in the Estuaries of Sundarbans during the period from January to December, 2014. The investigation was conducted on the Hariabhanga, Haringhata, Pasur and Shibsa estuarine area of the Sundarbans using a structured questionnaire. It was found that most of the fishermen were belonged to the age groups of 21 to 40 years (66%), represented by 76% Muslim. The family size of fishing community is usually consisted of 5-7 members and medium family is the predominant (56%) among the fishermen but in a joint family (78%). Over 82% of the fishermen primary occupation was fishing. About 36% of the fishermen were only could write the name or illiterate while 42% and 16 and 6% of the fishermen were primary, secondary and higher sec...
The investigation was conducted to evaluate the socioeconomic profile of the fishermen, using Best-Worst scale and Pearson’s correlation of Sugondha River, Bangladesh during the period of July 2015 to June 2016 by interviewing 200... more
The investigation was conducted to evaluate the socioeconomic profile of the fishermen, using Best-Worst scale and Pearson’s correlation of Sugondha River, Bangladesh during the period of July 2015 to June 2016 by interviewing 200 fishermen. In Sugondha River, 47% fishermen were engaged in fulltime fishing and 56% preferred group fishing. Maximum fishermen (49%) were middle aged and 56% were found Illiterate. 58% fishermen were lived with joint family and 17% fishermen had 8-above family members. Average annual income of most of the fishermen ranged from was BDT 56,000-70,000 (32%), where 30% had less than BDT 41,000. 48% women of the fishermen family supported their family by Poultry rearing. Age of fishers and income was positively correlated (r = 0.071) where income showed positive relationship with saving (r = 0.765; p<0.01), type (r = 0.561; p<0.01), fishing (r = 0.759; p<0.01), housing (r = 0.351; p<0.01), sanitation (r = 0.540; p<0.01) and health (r = 0.535; p&...
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An experiment was conducted to assess the open water fish diversity and fishing activities in the Padma River in Rajshahi, Bangladesh from the period of February 2012 to January 2013. A total number of 76 fish species belonging to 9... more
An experiment was conducted to assess the open water fish diversity and fishing activities in the Padma River in Rajshahi, Bangladesh from the period of February 2012 to January 2013. A total number of 76 fish species belonging to 9 orders, 22 families and 41 genera were recorded. The most dominant fish order was identified as Cypriniformes comprising 33% of total catch. About 27 endangered fish species were found in this area. Of the total fish species, 24% species were commonly found throughout the year. Twenty one species have been considered as threatened belonging to the Critically Endangered (33.33%), Vulnerable (10.60%) and Endangered (13.04%) of the total fish species. A total of 18 types of fishing gear (7 fishing nets, 6 fishing traps, 3 hooks and lines and 2 harpoons) were used for harvesting live fishes. The less availability of fishes is the major concern of declining biodiversity not only in the study area but also in the whole country.
Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue... more
Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue of Pacific abalone, Haliotis discus hannai. The full-length cDNA sequence of Hdh CA I was 1,417-bp in length, encoding a protein of 337 amino acids with molecular weight of 37.58 kDa. Hdh CA I sequence possessed a putative signal peptide of 22 amino acids and a CA catalytic function domain. The predicted protein shared 94 and 78% sequence identities with Haliotis gigantea and Haliotis tuberculata CA I, respectively. Results of phylogenetic analysis indicated that Hdh CA I was evolutionarily close to CA I of H. gigantea and H. tuberculata with high bootstrap values. Significantly higher levels of Hdh CA I mRNA transcript were found in mantle than other examined tissues. In situ hybridization results showed strong hybridization signals in epithelial ce...
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Temperature has crucial effects on gonadal development and reproduction of abalone. To understand the impact of thermal stress on molecular and physiological processes triggering the regulation of reproduction, changes in the mRNA... more
Temperature has crucial effects on gonadal development and reproduction of abalone. To understand the impact of thermal stress on molecular and physiological processes triggering the regulation of reproduction, changes in the mRNA expression of neuroendocrine genes encoding two abalone gonadotropin-releasing hormone (Hdh-GnRH, Hdh-GnRH-like peptide), GnRH receptor (Hdh-GnRH-R), Hdh-APGWamide, serotonin receptor (5-HThdh), and a heat shock protein HSP70 were examined in ganglia and testis of male Pacific abalone (Haliotis discus hannai). Abalone were exposed to low water temperature (LWT) and high water temperature (HWT) in early and peak breeding seasons for 7 days. Then, gonadosomatic index (GSI) was calculated, relative gene expression was measured by qRT-PCR, and levels of testosterone in hemolymph were also measured by ELISA during the peak breeding season. GSI did not show any significant changes during the early breeding season. However, it was significantly decreased in LWT- ...
The marbled flounder (Pseudopleuronectes yokohamae) is a commercial flatfish in East Asia. The aim of this study was to improve its sperm cryopreservation protocol based on the vitality assessment of 7-day and 1-year cryopreserved sperm.... more
The marbled flounder (Pseudopleuronectes yokohamae) is a commercial flatfish in East Asia. The aim of this study was to improve its sperm cryopreservation protocol based on the vitality assessment of 7-day and 1-year cryopreserved sperm. Four extenders (extender-1: sucrose solution; extender-2: glucose solution; extender-3: fish Ringer's solution; and extender-4: modified fish Ringer's solution) were tested with a combination of five cryoprotectants (CPAs) (dimethyl sulfoxide: Me2SO; glycerol: GLY; ethylene glycol: EG; propylene glycol: PG; and methanol: MeOH) at four different concentrations (5, 10, 12, and 15%). Fluorescent technique was applied to detect the plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of fresh and cryopreserved sperm specimens. Fresh sperm was diluted at a ratio of 1:2 (sperm:extender). Post-thaw motility of sperm cryopreserved using 15% Me2SO along with either extender-1 (86.0 ± 5.2%) or extender-2 (85.7 ± 7...
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Groupers are mostly found in the reef-associated marine habitat, of which some are pelagic and others are demersal. Recently, a grouper species called Oblique-banded grouper, Epinephelus radiatus (Day, 1868) was newly reported while... more
Groupers are mostly found in the reef-associated marine habitat, of which some are pelagic and others are demersal. Recently, a grouper species called Oblique-banded grouper, Epinephelus radiatus (Day, 1868) was newly reported while conducting research work on the availability of reef-associated fishes in St. Martin Island. This species had never been reported to occur not only from this coral reef area but also from the water area of Bangladesh. E. radiatus was easily identified following morphological traits, especially color pattern. The findings of the present study added new distributional range for this grouper species from Bangladeshi water.
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Pacific abalone (Haliotis discus hannai) is a commercially important high valued molluscan species. Its wild population has decreased in recent years. It is widely cultured in Korea. Traditional breeding programs have been implemented for... more
Pacific abalone (Haliotis discus hannai) is a commercially important high valued molluscan species. Its wild population has decreased in recent years. It is widely cultured in Korea. Traditional breeding programs have been implemented for hatchery production of abalone seeds. To obtain more genetic information for its molecular breeding program, a high-density linkage map and quantitative trait locus (QTL) for three growth-related traits was constructed for Pacific abalone. F1 cross population with two parents were sampled to construct the linkage map using genotyping by sequencing (GBS). A total of 664,630,534 clean reads and 56,686 SNPs were generated and 3,345 segregating SNPs were used to construct a consensus linkage map. The map spanned 1,747.023 cM with 18 linkage groups and an average interval of 0.55 cM. QTL analysis revealed two significant QTL in LG10 on the consensus linkage map of each growth-related trait. Both QTLs were located in the telomere region of the chromosome...
Pacific abalone (Haliotis discus hannai) is a highly commercial seafood in Southeast Asia. The aim of the present study was to improve the sperm cryopreservation technique for this valuable species using an antifreeze protein III... more
Pacific abalone (Haliotis discus hannai) is a highly commercial seafood in Southeast Asia. The aim of the present study was to improve the sperm cryopreservation technique for this valuable species using an antifreeze protein III (AFPIII). Post-thaw sperm quality parameters including motility, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), DNA integrity, fertility, hatchability, and mRNA abundance level of heat shock protein 90 (HSP90) were determined to ensure improvement of the cryopreservation technique. Post-thaw motility of sperm cryopreserved with AFPIII at 10 µg/mL combined with 8% dimethyl sulfoxide (DMSO) (61.3 ± 2.7%), 8% ethylene glycol (EG) (54.3 ± 3.3%), 6% propylene glycol (PG) (36.6 ± 2.6%), or 2% glycerol (GLY) (51.7 ± 3.0%) was significantly improved than that of sperm cryopreserved without AFPIII. Post-thaw motility of sperm cryopreserved with 2% MeOH and 1 µg/mL of AFPIII was also improved than that of sperm cryop...
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Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two... more
Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage...
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Insulin-like growth factor binding proteins (IGFBPs) are secreted proteins that play an important role in IGF regulation of growth and development of vertebrate and invertebrates. In this study, the IGFBP7 gene was cloned and... more
Insulin-like growth factor binding proteins (IGFBPs) are secreted proteins that play an important role in IGF regulation of growth and development of vertebrate and invertebrates. In this study, the IGFBP7 gene was cloned and characterized from mantle tissues of H. discus hannai, and designated as Hdh IGFBP7. The full-length cDNA sequence transcribed from the Hdh IGFBP7 gene was 1519-bp long with an open reading frame of 720-bp corresponding to a putative polypeptide of 239 amino acids. The molecular mass of its mature protein was approximately 23.44 KDa with an estimated isoelectric point (pI) of 5.35, and it shared significant homology with IGFBP7 gene of H. madaka. Hdh IGFBP7 has a characteristic IGFBP N-terminal domain (22–89 aa), a kazal-type serine proteinase inhibitor domain (77–128), and an immunoglobulin-like C2 domain (144–223). Furthermore, twelve cysteine residues and a signature motif of IGFBPs (XCGCCXXC) were found in its N-terminal domain. Phylogenetic analysis reveal...
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Insulin-like growth factor binding protein family is known to be involved in regulating biological actions of insulin-like growth factors (IGFs). In the present study, a full-length cDNA encoding the IGFBP-5 gene was cloned and... more
Insulin-like growth factor binding protein family is known to be involved in regulating biological actions of insulin-like growth factors (IGFs). In the present study, a full-length cDNA encoding the IGFBP-5 gene was cloned and characterized from the cerebral ganglion of Haliotis discus hannai. The 921-bp full-length sequence of Hdh IGFBP-5 cDNA transcript had an open reading frame of 411 bp encoding a predicted polypeptide of 136 amino acids, sharing high sequence identities with IGFBP-5 of H. diversicolor. The deduced Hdh IGFBP-5 protein contained a putative transmembrane domain (13-35 aa) in the N-terminal region. It also possessed a signature domain of IGFBP protein family (IB domain, 45-120 aa). Six cysteine residues (Cys-47, Cys-55, Cys-73, Cys-85, Cys-98, and Cys-118) in this cloned sequence could potentially form an intrachain disulfide bond. Phylogenetic analysis indicated that the Hdh IGFBP-5 gene was robustly clustered with IGFBP-5 of H. diversicolor. Tissue distribution ...