Your search for: 'author:("Gussago, Cristina")' has returned 7 results. (0.011s) Save search

Authors: Di Francesco, Andrea | Arosio, Beatrice | Gussago, Cristina | Dainese, Enrico | Mari, Daniela | D'Addario, Claudio | Maccarrone, Mauro

Article Type: Short Communication

Abstract: Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B4 , the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for …5-LOX in vulnerability to neurodegeneration. Show more

Keywords: Alzheimer's disease, biomarker, DNA methylation, fatty acid amide hydrolase, 5-lipoxygenase, peripheral blood mononuclear cells

DOI: 10.3233/JAD-130506

Citation: Journal of Alzheimer's Disease, vol. 37, no. 1, pp. 3-8, 2013

Authors: Arosio, Beatrice | Casati, Martina | Gussago, Cristina | Ferri, Evelyn | Abbate, Carlo | Scortichini, Valeria | Colombo, Elena | Rossi, Paolo Dionigi | Mari, Daniela

Article Type: Review Article

Abstract: As the European population gets older, the incidence of neurological disorders increases with significant impact on social costs. Despite differences in disease etiology, several brain disorders in the elderly (e.g., Alzheimer’s disease, vascular dementia, normal pressure hydrocephalus) share dementia as a common clinical feature. The current treatment for the majority of these diseases is merely symptomatic and does not modify the course of the illness. Symptoms of normal pressure hydrocephalus are the only ones that can be modified if they are recognized in time and treated appropriately. Therefore, an important clinical strategy may be disclosed by pathogenic pathways that can …be modified and to find drugs that can slow down or even arrest disease progression. Possibly a way to answer this question could be by re-examining all the molecules which have so far succeeded in improving many aspects of cognitive deterioration in some neurodegenerative conditions, that were not considered because of controversial opinions. The main purpose of this summary is to further substantiate the hypothesis that the pathway of adenosine type A2A receptor could be used as a potential target to develop new/old therapeutic strategies. Show more

Keywords: Adenosine, adenosine receptors, elderly, neurodegeneration

DOI: 10.3233/JAD-160324

Citation: Journal of Alzheimer's Disease, vol. 54, no. 2, pp. 417-425, 2016

Authors: Abbate, Carlo | Arosio, Beatrice | Galimberti, Daniela | Nicolini, Paola | Chiara, Lo Russo | Rossi, Paolo Dionigi | Ferri, Evelyn | Gussago, Cristina | Deriz, Milena | Fenoglio, Chiara | Serpente, Maria | Scarpini, Elio | Mari, Daniela

Article Type: Short Communication

Abstract: We describe a sporadic case of frontotemporal lobar degeneration, associated with the C9ORF72 mutation, with prominent behavioral changes and semantic deficits. Predominant deficits in naming, vocabulary, word comprehension, and face and object recognition emerged on neuropsychological assessment. Amnesia, behavioral changes, and isolated psychotic symptoms were also present. Hyposmia was an unspecific prodromal sign. Brain imaging showed basofrontal and temporopolar hypometabolism bilaterally, and predominantly left-sided atrophy. Levels of cerebrospinal fluid biomarkers (amyloid-β, tau and p-tau) were normal. This description further confirms the heterogeneous presentation of the C9ORF72 mutation.

Keywords: C9ORF72, frontotemporal lobar degeneration, hyposmia, phenotype, semantic dementia

DOI: 10.3233/JAD-132075

Citation: Journal of Alzheimer's Disease, vol. 40, no. 4, pp. 849-855, 2014

Authors: Gussago, Cristina | Arosio, Beatrice | Casati, Martina | Ferri, Evelyn | Gualandris, Federica | Tedone, Enzo | Nicolini, Paola | Rossi, Paolo Dionigi | Abbate, Carlo | Mari, Daniela

Article Type: Short Communication

Abstract: The line between vascular dementia (VaD) and Alzheimer's disease (AD) is often blurred. In this study we investigated whether adenosine A2A receptor (A2A R) expression can be used to differentiate between VaD and AD. We evaluated the expression of this receptor in the peripheral blood mononuclear cells of patients with VaD, mild cognitive impairment, AD, and controls. We found statistically significant lower levels of A2A R mRNA in VaD compared to AD subjects. These data suggest that A2A R expression may help in the differential diagnosis between VaD and AD.

Keywords: Adenosine receptors, Alzheimer's disease, biomarker, vascular dementia

DOI: 10.3233/JAD-131652

Citation: Journal of Alzheimer's Disease, vol. 40, no. 1, pp. 45-49, 2014

Authors: Asselineau, Delphine | Benlhassan, Khadija | Arosio, Beatrice | Mari, Daniela | Ferri, Evelyn | Casati, Martina | Gussago, Cristina | Tedone, Enzo | Annoni, Giorgio | Mazzola, Paolo | Piette, Francois | Belmin, Joel | Pariel, Sylvie | Bornand, Anne | Beaudeux, Jean-Louis | Doulazmi, Mohamed | Mariani, Jean | Bray, Dorothy H.

Article Type: Short Communication

Abstract: We investigated IL-10 and IL-6 production in amyloid-β (Aβ) stimulated peripheral blood mononuclear cells (PBMCs) in twenty Alzheimer’s disease (AD) patients with slow progression, eleven with fast progression, and twenty age-matched controls. Promoter polymorphisms in IL-10 (position -592, -819, -1082), IL-6 (-174), transforming growth factor-β1 (TGF-β1) (-10, -25), interferon-γ (IFN-γ ) (-874), and tumor necrosis factor-α (TNF-α ) (-308) genes were analyzed. IL-10 production after Aβ stimulation was high in PBMCs from slow decliners and almost completely abrogated in fast decliners. Association between AA IFN-γ low-producing genotype and fast progression was demonstrated. Investigations in a larger sample will clarify these …findings. Show more

Keywords: Alzheimer’s disease, disease progression, IFN-γ, interleukin-6, interleukin-10, peripheral blood mononuclear cells, single nucleotide polymorphisms

DOI: 10.3233/JAD-142832

Citation: Journal of Alzheimer's Disease, vol. 46, no. 4, pp. 837-842, 2015

Authors: Arosio, Beatrice | Abbate, Carlo | Galimberti, Daniela | Rossi, Paolo Dionigi | Inglese, Silvia | Fenoglio, Chiara | Ridolfi, Elisa | Gussago, Cristina | Casati, Martina | Tedone, Enzo | Ferri, Evelyn | Serpente, Maria | Scarpini, Elio | Mari, Daniela

Article Type: Short Communication

Abstract: We describe a case of late onset frontotemporal dementia carrying the g.1977_1980 delCACT (Thr272fs) mutation in progranulin (GRN) gene, characterized by a positive family history for dementia and a clinical phenotype resembling dementia with Lewy bodies. Symptoms included prominent visuospatial impairment, complex misidentification syndrome, visual zooptic hallucinations, hypersomnia, mental fluctuations, and signs of parkinsonism. The patient showed normal cerebrospinal fluid levels of amyloid-β, tau, and Ptau biomarkers, an asymmetric pattern of cerebral atrophy and hypoperfusion, and parietal hypometabolism. A major contributing factor to the diagnosis was the testing of plasmatic progranulin levels (extremely low), which prompted us to sequence GRN.

Keywords: GRN Thr272fs, frontotemporal dementia, phenotype, progranulin

DOI: 10.3233/JAD-130053

Citation: Journal of Alzheimer's Disease, vol. 35, no. 4, pp. 669-674, 2013

Authors: Tedone, Enzo | Arosio, Beatrice | Colombo, Federico | Ferri, Evelyn | Asselineau, Delphine | Piette, Francois | Gussago, Cristina | Belmin, Joel | Pariel, Sylvie | Benlhassan, Khadija | Casati, Martina | Bornand, Anne | Rossi, Paolo Dionigi | Mazzola, Paolo | Annoni, Giorgio | Doulazmi, Mohamed | Mariani, Jean | Porretti, Laura | Bray, Dorothy H. | Mari, Daniela

Article Type: Research Article

Abstract: Background: Age and short leukocyte telomeres have been associated with a higher risk of Alzheimer’s disease (AD). Inflammation is involved in AD and it is suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these processes. Objective: The aim is to correlate telomere length (TL) in peripheral blood mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β (Aβ)-stimulated PBMC. Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly (HE). After a two-year follow-up period, patients were retrospectively evaluated as slow-progressing (ADS) (Mini Mental …State Examination (MMSE) decline over the two years of follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was measured by flow cytometry and in vitro IL-10 production by enzyme-linked immunosorbent assay. Results: TL (mean±SD) for HE, ADS, and ADF was 2.3±0.1, 2.0±0.1, and 2.5±0.1 Kb, respectively. ADS showed a shorter TL compared to HE (p  = 0.034) and to ADF (p  = 0.005). MMSE decline correlated with TL in AD (R2  = 0.284; p  = 0.008). We found a significant difference in IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus 59.0±27.0; p  = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE showed a trend toward significance (47.1±25.4 versus 55.3±27.9; p  = 0.10). Conclusion: PBMC from ADF may be characterized by an impaired response induced by Aβ and by a reduced proliferative response responsible for the longer telomeres. TL might be a contributing factor in predicting the rate of AD progression. Show more

Keywords: Alzheimer’s disease, disease progression, interleukin-10, peripheral blood mononuclear cells, telomere

DOI: 10.3233/JAD-142808

Citation: Journal of Alzheimer's Disease, vol. 46, no. 3, pp. 761-769, 2015