Saroj Bhosle
Goa University, India, Microbiology, Department Member
Twenty-two alkaliphilic bacteria were isolated from spent waters of an agrochemical factory, Goa, India, and screened for exopolymer (EP) production on Horikoshi I/II media. Strain SB-D produced highly viscous cell-bound and extracellular... more
Twenty-two alkaliphilic bacteria were isolated from spent waters of an agrochemical factory, Goa, India, and screened for exopolymer (EP) production on Horikoshi I/II media. Strain SB-D produced highly viscous cell-bound and extracellular EP quantified by alcian blue adsorption assay. The 16S rRNA gene sequence of strain SB-D showed 97.34% similarity to Alkalihalobacillus lehensis strain MLBT. The strain maximally produced EP (10.3 g/L) at pH 10.3, 25 °C after 16 h growth on shaker, followed by 4 h at static condition with 4% inoculum in the presence of 3% glucose and 0.1% yeast extract. The EP precipitated using isopropanol contained carbohydrates (212 µg/mg), lipids (130 µg/mg), and proteins (86.16 µg/mg) as major components. Further, gas chromatography detected the presence of D-glucose, D-arabinose, D-mannose, D-galactose, D-xylose and D-ribose. Preliminary studies on EP characterization revealed emulsification and adhesive properties of the exopolymer.
Research Interests:
Iron being one of the most important elements required for metabolic activities of the bacteria has limited bioavailability. Therefore bacteria produce siderophores to sequester it from the environment. Halophilic and halotolerant adhered... more
Iron being one of the most important elements required for metabolic activities of the bacteria has limited bioavailability. Therefore bacteria produce siderophores to sequester it from the environment. Halophilic and halotolerant adhered bacteria from the mangrove ecosystem were screened for the production of siderophores. Out of a total of 16 bacterial isolates 81% showed siderophore production. The effect of zerovalent iron (ZVI) nanoparticles was studied on the production of these siderophores. Among a total of 13 siderophore producing isolates 46.15% showed an increase in the siderophore production in the presence of ZVI nanoparticles. The ZVI affected growth negatively in 7% of the bacterial isolates.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Copyright © 2012 Teja Gaonkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is... more
Copyright © 2012 Teja Gaonkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Bioremediation in natural ecosystems is dependent upon the availability of micronutrients and cofactors, of which iron is one of the essential elements. Under aerobic and alkaline conditions, iron oxidizes to Fe+3 creating iron deficiency. To acquire this essential growth-limiting nutrient, bacteria produce low-molecular-weight, high-affinity iron chelators termed siderophores. In this study, siderophore-producing bacteria from rhizosphere and nonrhizosphere areas of coastal sand dunes were isolated using a culture-dependent approach and were assigned to 8 different genera with the predominance of Bacillus sp. Studies on the ability of these isolates to grow on sodium benzoate revealed that a pigmented bacterial culture TMR2.13 identif...
The bacteriological quality of milk at different levels of collection was studied. Samples were collected in sterile containers at quarterly intervals. The samples were analysed for total vibal count, methylene blue reduction test (MBRT)... more
The bacteriological quality of milk at different levels of collection was studied. Samples were collected in sterile containers at quarterly intervals. The samples were analysed for total vibal count, methylene blue reduction test (MBRT) and California mastitis test (CMT). Subclinical mastitis was found in 23.8% of the animals. The average methylene blue reduction time decreased from the farm to the processing unit. The average counts were 2.67x10, 1.59x10, 9.23x10 and 2.35x10 cfu/ml at farmers’ field, milking utensils, collection centers and receiving dock (processing point), respectively. The milking methods, milk containers and time interval from the collection at the udder level to the receiving dock played a major role in the deterioration of the milk quality along with the climatic conditions prevalent in a particular season.
Research Interests:
The devclopment of a consortium compri sin g indi genous 11 0ra of th e coconut hu sks is reported. This co nsorti um wh ich co ul d he grown on hu sk leac hates has been used as inoculum for the relling process under laboratory condition... more
The devclopment of a consortium compri sin g indi genous 11 0ra of th e coconut hu sks is reported. This co nsorti um wh ich co ul d he grown on hu sk leac hates has been used as inoculum for the relling process under laboratory condition s. The eonsortium has heen deve loped on hu sk leac hates with sa linity adj usted to 6.5 ppt after an acc limation period of ninety days with three subcultures. Whcn platcd on nutri ent agar. 10 different bacterial cu ltures coul d be isolated among which gram negat i vc cocco haci ll ary forms ha ve heen th e most predominant. The con sortium is grown on HLM (Husk leachate medium) and inocu lated on coconut hu sb steeped for relling in 5 and 10 per cen t co ncentrati ons in tanks . It is found th aI th e consorti um coo ld redu ce the retling pe riod to three mon th s. The environmental parameters such as pH. sal inity and tem perature ha ve heen Illonitored I'm a period of three months. It is ohserved th at th e fibre cou ld he separated fro...
Research Interests:
Studies were carried out to evaluate the geotechnical properties of vemicomposts (VCs) such as porosity, void ratio, density, air content, water holding capacity and particle size distribution (PSD) prepared from sugar industrial waste... more
Studies were carried out to evaluate the geotechnical properties of vemicomposts (VCs) such as porosity, void ratio, density, air content, water holding capacity and particle size distribution (PSD) prepared from sugar industrial waste press mud (PM) using surface feeders Eisenia fetida, Eudrilus eugeniae and the deep burrower Megascolex megascolex. A comparison of the geotechnical properties of 40-day samples of VCs from field trials by a core cutter showed that the VC using M. megascolex was superior to E. eugeniae and E. fetida VCs in terms of water and air content and percentage void. The PSD of PM was higher than industrial soil, while M. megascolex VC > E. fetida VC > E. eugeniae VC (P E. fetida > M. megascolex. This study clearly indicates that the indigenous deep burrower M. megascolex can be used in the vermicomposting of PM while also enhancing the geotechnical properties, soil aggregation and water holding capacity of a VC. _______________________________________...
Research Interests:
Research Interests:
Research Interests: Microbiology, Epidemiology, Biology, Virology, Molecular Microbiology, and 15 moreMedicine, Food Microbiology, India, Humans, Animals, Listeria monocytogenes, Larva, Pulsed field gel electrophoresis (PFGE), Listeriosis, Moths, Listeria, Medical Sciences and Medicine, Serotyping, Serotype, and Serogroup
Collections of gram positive bacteria from coastal sand dune vegetation, Ipomoea pes-caprae showed a predominance of orange pigmented colonies of Microbacterium arborescens -AGSB. The pigment was identified using a combinati on of... more
Collections of gram positive bacteria from coastal sand dune vegetation, Ipomoea pes-caprae showed a predominance of orange pigmented colonies of Microbacterium arborescens -AGSB. The pigment was identified using a combinati on of UV/visible spectral data and HPLC retention time as a lycopene type carotenoid pigment with λmax at 468 nms. These bacteria may be accumulating carotenoids as part of their responses to various environmental stresses and thus aiding their survival in this stressed habitat.
Research Interests:
Research Interests:
Coastal sand dunes are a nutrient-limited ecosystem. The coastal dune ecosystem experiences severe stresses in the form of salt spray, sand burial, low moisture content, high light intensity, wind exposure, soil salinity, and nutrient... more
Coastal sand dunes are a nutrient-limited ecosystem. The coastal dune ecosystem experiences severe stresses in the form of salt spray, sand burial, low moisture content, high light intensity, wind exposure, soil salinity, and nutrient deficiency (C, N, P, and K). Sand dune plants or psammophytes as they are called are naturally adapted to these stress conditions. The bacteria associated with the vegetation on the dunes, i.e., rhizosphere, and adhered have adapted to this stressed environment by producing biologically active metabolites which have sustained the survival of the vegetation on the dunes. These bacteria produce important plant growth-promoting metabolites which have been harnessed for their use in agricultural crops.
Research Interests:
Research Interests:
Pseudomonas nitroreducens TSB.MJ10 exhibiting growth and bioemulsifier production with 0.5% sodium benzoate as the sole carbon source was isolated from a mangrove ecosystem in the vicinity of a petroleum pump. The bioemulsifier is a... more
Pseudomonas nitroreducens TSB.MJ10 exhibiting growth and bioemulsifier production with 0.5% sodium benzoate as the sole carbon source was isolated from a mangrove ecosystem in the vicinity of a petroleum pump. The bioemulsifier is a lipopeptide that is stable over a pH range of 5-11 and a temperature range of 20-90°C and showed emulsifying activity in the presence of relatively high NaCl concentrations (up to 25%). The bioemulsifier formed stable emulsions with aliphatic (hexadecane, n-heptane, cyclohexane), aromatic (xylene, benzene, toluene) and petroleum (gasoline, diesel, kerosene, crude oil) compounds. It exhibited a maximum emulsification activity with weathered crude oil (97%) and was capable of transforming the rheological behavior of the pseudoplastic to a Newtonian fluid. The results reveal the potential of the bioemulsifier for use in bioremediation of hydrocarbons in marine environments and in enhanced oil recovery.
Research Interests: Chemistry, Petroleum, Carbon, Rheology, Medicine, and 15 moreMultidisciplinary, Pseudomonas, Temperature, Avicennia marina, HYDROCARBONS, Diesel Fuel, Ecosystem, Toluene, Sodium Benzoate, Sodium Chloride, Kerosene, Lipopeptides, Molecular Sequence Data, Bioresource technology, and emulsifying agents
Research Interests:
Research Interests:
Research Interests:
Zerovalent iron (ZVI) nanoparticles are gaining popularity in bioremediation of contaminated ground water and antimicrobial studies. In this study, ZVI nanoparticles were synthesized by borohydride method. The effect of these... more
Zerovalent iron (ZVI) nanoparticles are gaining popularity in bioremediation of contaminated ground water and antimicrobial studies. In this study, ZVI nanoparticles were synthesized by borohydride method. The effect of these nanoparticles to alter the cell surface hydrophobicity of mangrove bacteria was studied by bacterial adhesion to hydrocarbon assay. The effect of these nanoparticles on the growth and extracellular polymeric substances (EPS) of a novel bacterial strain Halobacillus trueperi MXM-16 from mangroves was evaluated by growing the culture in the presence of ZVI nanoparticles and SEM. The change in the emulsifying ability of the cell-free supernatant of Halobacillus trueperi MXM-16 when grown in media amended with ZVI nanoparticles was also investigated by spectrophotometric analysis.
Research Interests:
... Pseudomonas aeruginosa strain USS25 NCIM-5224 Upal Roy* and Saroj Bhosle ... Based on the IR, NMR spectra and GC-MS analysis, the isolated product was identified as monobutyltin dichloro hydride. Copyright 2005 John Wiley... more
... Pseudomonas aeruginosa strain USS25 NCIM-5224 Upal Roy* and Saroj Bhosle ... Based on the IR, NMR spectra and GC-MS analysis, the isolated product was identified as monobutyltin dichloro hydride. Copyright 2005 John Wiley & Sons, Ltd. ...
Research Interests:
Obligate alkaliphilic bacteria (28 strains) were isolated from various estuarine ecosystems of Goa. Most of these strains were found to be Gram positive, motile rods, capable of growth in aerobic condition upto pH 12, exhibiting optimum... more
Obligate alkaliphilic bacteria (28 strains) were isolated from various estuarine ecosystems of Goa. Most of these strains were found to be Gram positive, motile rods, capable of growth in aerobic condition upto pH 12, exhibiting optimum growth at pH 10.5. Isolates exhibited high buffering capacity confirming their alkaliphilic nature. Significantly high difference was noted in the buffering capacities of the alkaliphiles and the neutrophile indicating their ability to maintain their internal pH. The obligate alkaliphile A52 exhibited highest cytoplasmic buffering capacity of 8,500 nanomoles OH- ions/pH unit/mg protein. The isolates also showed amylase (39%), protease (50%) and lipase (100%) activity under alkaline conditions. The present study depicts that such alkaliphilic bacteria also play an important role in the mineralization of organic matter under high pH conditions in natural ecosystems.
Research Interests:
Research Interests:
Marine bacteria, known to produce wide range of molecules that are beneficial to animals as well as to human beings, were screened for the presence of alpha linolenic acid (9,12,15-octadeca trienioc acid). The lipid and protein... more
Marine bacteria, known to produce wide range of molecules that are beneficial to animals as well as to human beings, were screened for the presence of alpha linolenic acid (9,12,15-octadeca trienioc acid). The lipid and protein concentrations of predominant bacterial isolates, obtained from coastal marine sediment were determined. Out of twenty isolates; eight bacterial isolates with higher lipid-protein ratio (more than 0.5), were grown in mineral salt medium with sodium acetate as carbon source as well as in nutrient broth. Their lipid (triglyceride, sterol, fatty acid, glycolipid and phospholipid) and fatty acid (mainly C-18 series) profiles were analyzed. Only four bacterial isolates depicted significant conversion efficacy for alpha linolenic acid (more than 25%) when they were grown in sodium acetate media. Such bacteria can be used as supplement to enrich the animal feed with the required fatty acid.
Research Interests:
Research Interests:
Research Interests:
Listeria monocytogenes is an important pathogen of public health significance. Isolation and characterization of L. monocytogenes was attempted from the different components of pork production system in the west coast region of India. A... more
Listeria monocytogenes is an important pathogen of public health significance. Isolation and characterization of L. monocytogenes was attempted from the different components of pork production system in the west coast region of India. A total of 501 samples were collected from pig rearing environment (n=146), sick and healthy pigs (n=199), slaughter house environment (n=62), raw pork (n=37) and pork products (n=57). Listeria spp. were isolated from 31 (6.18%) samples. The isolates were further subjected to biochemical and genotypic characterization. Out of 31 isolates, 11(2.19%) isolates were confirmed as L. monocytogenes, 8 (1.59%) as L. innocua, 6 (1.19%) as L. seeligeri, 3 (0.59%) as L. ivanovii and 3 (0.59%) as L. welshimeri. Multiplex PCR based serotyping revealed 10 L. monocytogenes isolates to be of serovar group 4b, 4d, 4c, while, one isolate was of serovar group 1/2b, 3b, 7. PFGE analysis revealed clonality of the strains prevalent in pork production chain and were similar ...
Research Interests:
Pisolithus sp. PT1 was isolated from mushroom found in ectomycorrhizal association with Acacia mangium growing in mining affected region. The fungal isolate was characterised by morphological and molecular methods. The isolate produced... more
Pisolithus sp. PT1 was isolated from mushroom found in ectomycorrhizal association with Acacia mangium growing in mining affected region. The fungal isolate was characterised by morphological and molecular methods. The isolate produced brown phenolics compound in GMSM culture broth. The isolate produced more phenolics in the late growth phase and under submerged growth conditions. Antagonism activity of phenolics was prominent against Fusarium sp.. Spore pigment and culture filtrate with pH 3.0, but not at pH 5.0, significantly arrested the growth. Macrophomina phaesiolus and Sclerotia rolsii were not affected with any of the pigments. This study has given conclusive results that phenolics produced by this Pisolithus sp. PT1 isolate are potential antibiotic compounds and maximum production was between 20-60 days and under submerged growth conditions.
Pisolithus sp. was isolated from ectomycorrhizal association with Acacia mangium growing in mining affected forest area along the west coast of India. The in vitro growth response and phenolics production by the isolate was checked using... more
Pisolithus sp. was isolated from ectomycorrhizal association with Acacia mangium growing in mining affected forest area along the west coast of India. The in vitro growth response and phenolics production by the isolate was checked using mineral medium for various edaphic factors prevailing in rejects of iron ore mines. Phosphatecitrate buffer (0.047 M) was found suitable for maintaining the pH of the medium. The isolate survived at 10 and 42oC. Interestingly, the isolate showed growth in the presence of 10,000 ppm Mn and appeared to be the most Mn-tolerant. The isolate preferred ferric form of iron over ferrous. The optima of all the variables for phenolics production were found at higher values than that required for the growth of isolate, significantly revealing the enhanced elaboration of phenolics at stress conditions. Maximum yields of phenolics obtained were 149.91, 164.07 and 61.34 μg/20ml with 1.5% NaCl, 37oC and 5% PEG, respectively. The wide tolerance and elaboration of p...
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
... Pseudomonas aeruginosa strain USS25 NCIM-5224 Upal Roy* and Saroj Bhosle ... Based on the IR, NMR spectra and GC-MS analysis, the isolated product was identified as monobutyltin dichloro hydride. Copyright 2005 John Wiley... more
... Pseudomonas aeruginosa strain USS25 NCIM-5224 Upal Roy* and Saroj Bhosle ... Based on the IR, NMR spectra and GC-MS analysis, the isolated product was identified as monobutyltin dichloro hydride. Copyright 2005 John Wiley & Sons, Ltd. ...
Research Interests:
Research Interests:
Most bacteria and their enzymes are destroyed or inactivated in the presence of organic solvents. Organic solvent tolerant bacteria are a relatively novel group of extremophilic microorganisms that combat these destructive effects and... more
Most bacteria and their enzymes are destroyed or inactivated in the presence of organic solvents. Organic solvent tolerant bacteria are a relatively novel group of extremophilic microorganisms that combat these destructive effects and thrive in the presence of high concentrations of organic solvents as a result of various adaptations. These bacteria are being explored for their potential in industrial and environmental biotechnology, since their enzymes retain activity in the presence of toxic solvents. This property could be exploited to carry out bioremediation and biocatalysis in the presence of an organic phase. Because a large number of substrates used in industrial chemistry, such as steroids, are water-insoluble, their bioconversion rates are affected by poor dissolution in water. This problem can be overcome by carrying out the process in a biphasic organic-aqueous fermentation system, wherein the substrate is dissolved in the organic phase and provided to cells present in t...
Research Interests:
Bioremediation in natural ecosystems is dependent upon the availability of micronutrients and cofactors, of which iron is one of the essential elements. Under aerobic and alkaline conditions, iron oxidizes to Fe(+3) creating iron... more
Bioremediation in natural ecosystems is dependent upon the availability of micronutrients and cofactors, of which iron is one of the essential elements. Under aerobic and alkaline conditions, iron oxidizes to Fe(+3) creating iron deficiency. To acquire this essential growth-limiting nutrient, bacteria produce low-molecular-weight, high-affinity iron chelators termed siderophores. In this study, siderophore-producing bacteria from rhizosphere and nonrhizosphere areas of coastal sand dunes were isolated using a culture-dependent approach and were assigned to 8 different genera with the predominance of Bacillus sp. Studies on the ability of these isolates to grow on sodium benzoate revealed that a pigmented bacterial culture TMR2.13 identified as Pseudomonas aeruginosa showed growth on mineral salts medium (MSM) with 2% of sodium benzoate and produced a yellowish fluorescent siderophore identified as pyoverdine. This was inhibited above 54 μM of added iron in MSM with glucose without a...