Diabetes affects the homeostasis of the circulatory system. Crassocephalum crepidioides Benth S. ... more Diabetes affects the homeostasis of the circulatory system. Crassocephalum crepidioides Benth S. Moore (Asteraceae) is an edible plant locally used in the treatment of wounds, stomach ulcer, and skin-related conditions in Africa and some other parts of the world. This study investigated the effects of C. crepidioides leaf methanol extract and fractions on blood coagulation profile of diabetic Wistar rats. The effect of 100 mg/kg body weight of the methanol extract and partitioned fractions of C. crepidioides on blood coagulation profile of STZ-induced diabetic rats were initially evaluated, while graded concentrations (50–200 mg/kg body weight) of the aqueous and hexane fractions were further tested in diabetic rats against standard drugs aspirin (anticoagulant) and metformin (antidiabetic). Rats were allocated into groups (n = 6) and administration was done orally, once daily for 2 weeks. The methanol extract and fractions of C. crepidioides at concentrations of 50, 100, and 200 mg...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
There is a growing scientific evidence of the health-enhancing benefits of fermented food and bev... more There is a growing scientific evidence of the health-enhancing benefits of fermented food and beverages, especially in cancer prevention and treatment. Ogiri-egusi is an indigenously fermented condiment consumed in some West African countries, and prepared mainly from natural fermentation of melon (Citrullus vulgaris) seeds. The condiment has been previously reported to possess anti-oxidative and anti hyperlipid emic properties. In this study, different concentrations of ogiri-egusi extracts were exposed to actively dividing cells from Allium cepa meristematic roots and Saccharomyces cerevisiae for the antimitotic and antiproliferative investigations, respectively. There was a statistically significant reduction in mitotic activities of the treated A. cepa meristematic cells compared to the untreated cells. The mitotic index (MI) of the untreated cells was 28.38 ± 4.58. At a concentration of 1.0 mg/ml, the extracts induced a reduction in the MI to 6.02 ± 2.74 and 4.94 ± 0.61 in the...
Objective: There is a growing scientific evidence of the health-enhancing benefits of fermented f... more Objective: There is a growing scientific evidence of the health-enhancing benefits of fermented food especially in cancer treatment and prevention. Fermented African locust beans (FALB) are a condiment with many medicinal activities and consume in many West African countries. Breast cancer is the most common cancer among women globally. This study investigated the cytotoxicity of FALB extracts on breast cancer (MCF-7) cells overtime and at different concentrations. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to quantify the extent of cytotoxicity of the extracts. Results: The aqueous extract of FALB had an half maximal inhibitory concentration (IC50) of 1.51 and 0.98 mg/mL on exposure for 24 and 48 h, respectively, against MCF-7 cells. Comparatively, the IC50 obtained for the same extract against normal human fibroblasts was 1.90 and 1.37 mg/mL, respectively. Conclusion: The results obtained here suggest some measure of selective c...
Hepatoprotective effect of Garcinia kola seed extract on oxidative stress and hepatotoxicity biom... more Hepatoprotective effect of Garcinia kola seed extract on oxidative stress and hepatotoxicity biomarkers of rats exposed to antitubercular drugs was investigated to evaluate its potential to ameliorate drug-induced hepatotoxicity. Six groups of five animals each were used. Combination of Isoniazid (7.5 mg/kg bodyweight) and Rifampicin (10 mg/kg bodyweight) was administered intraperitoneally to a group. Drug combination was co-administered with G. kola seed extracts (40, 60 and 80 mg/kg bodyweight) to three other groups for 35 days. Control group received saline and the last group received G. kolaalone (60 mk/kg bodyweight). Plasma oxidative stress biomarkers (superoxide dismutase, glutathione, glutathione peroxidase, catalase and malondialdehyde) and hepatotoxicity biomarkers (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase) were assayed after treatment. The antitubercular drugs significantly increased levels of hepatotoxicity biomarkers. The increase wa...
The multifunctional nature of antioxidant peptides makes them more attractive candidates as dieta... more The multifunctional nature of antioxidant peptides makes them more attractive candidates as dietary ingredients in health maintenance. Therefore, food protein-derived antioxidant peptides are continuously investigated. This study investigated the in vitro antioxidant properties of hydrolysate and ultrafiltered peptide fractions of Pachymelania aurita and Tympanatonus fuscatus var radula-two commonly consumed marine molluscs known as periwinkles in southern Nigeria. Simulated gastrointestinal digestion (SGID) of soluble proteins of T. fuscatus and P. aurita was carried out using pepsin, trypsin and chymotrypsin, and the SGID hydrolysates were fractionated using a 3 kDa membrane filter. The hydrolysates and their fractions were investigated for anti-lipid peroxidation, hydroxyl radical scavenging activity (HRSA), ferric reducing antioxidant property (FRAP) and metal chelation activity, and they demonstrated clear antioxidant properties in all the assay models used. Low molecular weigh...
The multifunctional nature of antioxidant peptides makes them more attractive candidates as dieta... more The multifunctional nature of antioxidant peptides makes them more attractive candidates as dietary ingredients in health maintenance. Therefore, food protein-derived antioxidant peptides are continuously investigated. This study investigated the in vitro antioxidant properties of hydrolysate and ultrafiltered peptide fractions of Pachymelania aurita and Tympanatonus fuscatus var radula-two commonly consumed marine molluscs known as periwinkles in southern Nigeria. Simulated gastrointestinal digestion (SGID) of soluble proteins of T. fuscatus and P. aurita was carried out using pepsin, trypsin and chymotrypsin, and the SGID hydrolysates were fractionated using a 3 kDa membrane filter. The hydrolysates and their fractions were investigated for anti-lipid peroxidation, hydroxyl radical scavenging activity (HRSA), ferric reducing antioxidant property (FRAP) and metal chelation activity, and they demonstrated clear antioxidant properties in all the assay models used. Low molecular weigh...
Globally fermented foods form an integral part of the staple diet of people. Fermented Citrullus ... more Globally fermented foods form an integral part of the staple diet of people. Fermented Citrullus vulgaris (FCV) (ogiri) seeds is a nutritious natural fermented condiment that features frequently in West African diets as a spice. This study investigated the antihyperlipidaemic potential of this condiment in tyloxapol-induced hyperlipidaemic rats with reference to that of fluvastatin, a standard antilipidaemic drug. The antioxidant potential of the condiment was also examined using different relevant in vitro assays. Albino rats were divided into six groups (n=5), based on the diet and treatment received. The groups were standard feed (control); FCV supplemented feed; standard feed with tyloxapol administered at the end of the experimental period (tyloxapol control); FCV supplemented feed and tyloxapol at the end of the experimental period; standard feed with fluvastatin sodium (40 mg/kg and 80 mg/kg body weight separately) followed by tyloxapol. The results revealed that administrati...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
International journal of biochemistry and molecular biology, 2019
This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone... more This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita, against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro. The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of T.fuscatus (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC50 = 96.48 ± 1.36 μg/ml, HCC 1395 IC50 = 61.44 ± 2.45 μg/ml, Hep2 IC50 = 0.52 ± 0.36 μg/ml) and also had the highest selectivity index of 4.94, 7.78 and 921.97 for DU145, HCC 1395...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
Diabetes affects the homeostasis of the circulatory system. Crassocephalum crepidioides Benth S. ... more Diabetes affects the homeostasis of the circulatory system. Crassocephalum crepidioides Benth S. Moore (Asteraceae) is an edible plant locally used in the treatment of wounds, stomach ulcer, and skin-related conditions in Africa and some other parts of the world. This study investigated the effects of C. crepidioides leaf methanol extract and fractions on blood coagulation profile of diabetic Wistar rats. The effect of 100 mg/kg body weight of the methanol extract and partitioned fractions of C. crepidioides on blood coagulation profile of STZ-induced diabetic rats were initially evaluated, while graded concentrations (50–200 mg/kg body weight) of the aqueous and hexane fractions were further tested in diabetic rats against standard drugs aspirin (anticoagulant) and metformin (antidiabetic). Rats were allocated into groups (n = 6) and administration was done orally, once daily for 2 weeks. The methanol extract and fractions of C. crepidioides at concentrations of 50, 100, and 200 mg...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
There is a growing scientific evidence of the health-enhancing benefits of fermented food and bev... more There is a growing scientific evidence of the health-enhancing benefits of fermented food and beverages, especially in cancer prevention and treatment. Ogiri-egusi is an indigenously fermented condiment consumed in some West African countries, and prepared mainly from natural fermentation of melon (Citrullus vulgaris) seeds. The condiment has been previously reported to possess anti-oxidative and anti hyperlipid emic properties. In this study, different concentrations of ogiri-egusi extracts were exposed to actively dividing cells from Allium cepa meristematic roots and Saccharomyces cerevisiae for the antimitotic and antiproliferative investigations, respectively. There was a statistically significant reduction in mitotic activities of the treated A. cepa meristematic cells compared to the untreated cells. The mitotic index (MI) of the untreated cells was 28.38 ± 4.58. At a concentration of 1.0 mg/ml, the extracts induced a reduction in the MI to 6.02 ± 2.74 and 4.94 ± 0.61 in the...
Objective: There is a growing scientific evidence of the health-enhancing benefits of fermented f... more Objective: There is a growing scientific evidence of the health-enhancing benefits of fermented food especially in cancer treatment and prevention. Fermented African locust beans (FALB) are a condiment with many medicinal activities and consume in many West African countries. Breast cancer is the most common cancer among women globally. This study investigated the cytotoxicity of FALB extracts on breast cancer (MCF-7) cells overtime and at different concentrations. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to quantify the extent of cytotoxicity of the extracts. Results: The aqueous extract of FALB had an half maximal inhibitory concentration (IC50) of 1.51 and 0.98 mg/mL on exposure for 24 and 48 h, respectively, against MCF-7 cells. Comparatively, the IC50 obtained for the same extract against normal human fibroblasts was 1.90 and 1.37 mg/mL, respectively. Conclusion: The results obtained here suggest some measure of selective c...
Hepatoprotective effect of Garcinia kola seed extract on oxidative stress and hepatotoxicity biom... more Hepatoprotective effect of Garcinia kola seed extract on oxidative stress and hepatotoxicity biomarkers of rats exposed to antitubercular drugs was investigated to evaluate its potential to ameliorate drug-induced hepatotoxicity. Six groups of five animals each were used. Combination of Isoniazid (7.5 mg/kg bodyweight) and Rifampicin (10 mg/kg bodyweight) was administered intraperitoneally to a group. Drug combination was co-administered with G. kola seed extracts (40, 60 and 80 mg/kg bodyweight) to three other groups for 35 days. Control group received saline and the last group received G. kolaalone (60 mk/kg bodyweight). Plasma oxidative stress biomarkers (superoxide dismutase, glutathione, glutathione peroxidase, catalase and malondialdehyde) and hepatotoxicity biomarkers (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase) were assayed after treatment. The antitubercular drugs significantly increased levels of hepatotoxicity biomarkers. The increase wa...
The multifunctional nature of antioxidant peptides makes them more attractive candidates as dieta... more The multifunctional nature of antioxidant peptides makes them more attractive candidates as dietary ingredients in health maintenance. Therefore, food protein-derived antioxidant peptides are continuously investigated. This study investigated the in vitro antioxidant properties of hydrolysate and ultrafiltered peptide fractions of Pachymelania aurita and Tympanatonus fuscatus var radula-two commonly consumed marine molluscs known as periwinkles in southern Nigeria. Simulated gastrointestinal digestion (SGID) of soluble proteins of T. fuscatus and P. aurita was carried out using pepsin, trypsin and chymotrypsin, and the SGID hydrolysates were fractionated using a 3 kDa membrane filter. The hydrolysates and their fractions were investigated for anti-lipid peroxidation, hydroxyl radical scavenging activity (HRSA), ferric reducing antioxidant property (FRAP) and metal chelation activity, and they demonstrated clear antioxidant properties in all the assay models used. Low molecular weigh...
The multifunctional nature of antioxidant peptides makes them more attractive candidates as dieta... more The multifunctional nature of antioxidant peptides makes them more attractive candidates as dietary ingredients in health maintenance. Therefore, food protein-derived antioxidant peptides are continuously investigated. This study investigated the in vitro antioxidant properties of hydrolysate and ultrafiltered peptide fractions of Pachymelania aurita and Tympanatonus fuscatus var radula-two commonly consumed marine molluscs known as periwinkles in southern Nigeria. Simulated gastrointestinal digestion (SGID) of soluble proteins of T. fuscatus and P. aurita was carried out using pepsin, trypsin and chymotrypsin, and the SGID hydrolysates were fractionated using a 3 kDa membrane filter. The hydrolysates and their fractions were investigated for anti-lipid peroxidation, hydroxyl radical scavenging activity (HRSA), ferric reducing antioxidant property (FRAP) and metal chelation activity, and they demonstrated clear antioxidant properties in all the assay models used. Low molecular weigh...
Globally fermented foods form an integral part of the staple diet of people. Fermented Citrullus ... more Globally fermented foods form an integral part of the staple diet of people. Fermented Citrullus vulgaris (FCV) (ogiri) seeds is a nutritious natural fermented condiment that features frequently in West African diets as a spice. This study investigated the antihyperlipidaemic potential of this condiment in tyloxapol-induced hyperlipidaemic rats with reference to that of fluvastatin, a standard antilipidaemic drug. The antioxidant potential of the condiment was also examined using different relevant in vitro assays. Albino rats were divided into six groups (n=5), based on the diet and treatment received. The groups were standard feed (control); FCV supplemented feed; standard feed with tyloxapol administered at the end of the experimental period (tyloxapol control); FCV supplemented feed and tyloxapol at the end of the experimental period; standard feed with fluvastatin sodium (40 mg/kg and 80 mg/kg body weight separately) followed by tyloxapol. The results revealed that administrati...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
International journal of biochemistry and molecular biology, 2019
This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone... more This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita, against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro. The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of T.fuscatus (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC50 = 96.48 ± 1.36 μg/ml, HCC 1395 IC50 = 61.44 ± 2.45 μg/ml, Hep2 IC50 = 0.52 ± 0.36 μg/ml) and also had the highest selectivity index of 4.94, 7.78 and 921.97 for DU145, HCC 1395...
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts o... more This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation ...
Uploads