Alba Lombraña

The Sardinian Germplasm Bank (BG-SAR) is a facility of the Hortus Botanicus Karalitanus (HBK), which belongs to the University of Cagliari (Italy). Its main objective is the conservation, study and management of the germplasm of Sardinian endemic, threatened and policy species (i.e., species inserted in the Habitat Directive 92/43/EEC, CITES and Bern convention), taxa from insular territories of the Mediterranean region, as well as Crop Wild Relatives (CWR), landraces, useful plants and plant remains. A summary of the procedures implemented by BG-SAR for the ex situ conservation, some international scientific results achieved, and some research projects at regional, national and international level in which the bank is involved, are reported in this work, with the main aim to highlight how a germplasm bank can be considered an important tool for the preservation of plant biodiversity. This paper allows to make a reflection about the importance of the germplasm banks, as well as thei...

Grape pomace represents 60%, by weight, of the solid side-streams of the wine-making process. The quantities produced, seasonality, and the presence of polyphenols pose economic and environmental issues that require proper management approaches based on the principles of sustainability and circular economy. The present work focuses on the combined application of solid–liquid extraction of polyphenols from ground grape pomace using a hydroethanolic mixture and the composting of the exhausted pomace. The obtained results support the possibility of recovering approximately 76.5 g of extract per kg of dry grape pomace (or 1.8 g of total phenols per kg of dry grape pomace). The composting process was not affected by the extraction process. On the contrary, the composting process was enhanced by the pomace particle size reduction, in terms of final biostability and content of humic acids.

In this contribution, new data concerning the distribution of vascular flora alien to Italy are presented. It includes new records, confirmations, exclusions, and status changes for Italy or for Italian administrative regions. Nomenclatural and distribution updates published elsewhere are provided as Suppl. material 1.

Carignan is a black grape cultivar widely planted throughout the western Mediterranean Basin. The grape faces significant viticultural hazards such as soil salinization, which affects about 6% of the world’s total land area. The search for salt tolerance genotypes to be introduced in crossbreeding programs and obtaining new cultivars is a key factor. The seed germination and salt tolerance of Carignan were studied from different coastal vineyards across the Mediterranean Basin, and as well as whether the distance from the sea affected germination and salt tolerance. Carignan seeds, independently of the temperature and distance from the sea, germinated more than 50% under 125 mM NaCl concentrations. Seed recovery was elevated, including the capacity of gemination after high salt exposure (500 mM NaCl). The results on germination behavior related to the distance from the sea showed that all tested vineyards, except for the one farthest from the sea, had similar germination responses. ...

Gentiana lutea L. subsp. lutea is a geophyte that occurs in the central-southern European mountains. We investigated its germination ecophysiology with the main aim of evaluating seed germination behaviour under controlled and natural conditions. Seeds from four natural sites were tested for germination requirements under a range of different temperatures, and by comparing the results with those obtained under both cold stratification at 5 °C and treatment with GA3. We simultaneously carried out germination tests at each collecting site, and soil temperatures were recorded. Our results indicate that germination under controlled conditions is limited by high temperatures (25 °C), below which GA3 has a remarkable effect on germination percentages. Cold stratification did not have an influence on the germination response, thus suggesting that a temperature of 5 °C is not sufficient to break seed dormancy; this result was supported by soil temperatures. Gentiana lutea created a short-te...

The Aichi Biodiversity Target 12 aims to prevent the extinction risk of known threatened species and to improve their conservation status by 2020. We present the integrated strategy implemented in the last 10 years for the keystone plant species of Sardinia (Italy, W. Mediterranean Basin), which includes the following activities: conservation status assessment (following the IUCN protocol), ex situ conservation, in situ monitoring and active protection measures. To date, an average of 51.8% of keystone plant species have been subjected to the latter first three activities while, due to the higher costs, only few active conservation measures have been carried out. Considering the activities realised since 2004, we have also predicted the conservation effectiveness towards 2020 and have elaborated an index to evaluate it. Halfway through the strategic plan, we argue that more efforts are needed to guarantee the effective conservation of all threatened plants in Sardinia.

In this contribution, new data concerning the distribution of native vascular flora in Italy are presented. It includes new records, confirmations, and status changes to the Italian administrative regions. Nomenclatural and distribution updates, published elsewhere, and corrigenda are provided as Suppl. material 1.

Knowledge of the levels of genetic diversity and of the spatial genetic structure of plant species is important to ensure their effective management and conservation, especially in the case of endangered species. <i>Gentiana lutea</i> L. subsp. <i>lutea</i> is a long-lived plant which occurs in central and southern European mountains. It has a long-standing history of human exploitation, mainly in the liqueur and in the pharmaceutical industries and it is currently listed in the EU Habitats Directive 92/43/EEC Annex V. Mainly due to a prolonged root harvesting, its current distribution range in Sardinia consists of only a few groups of individuals limited to small areas of the Gennargentu massif (Central-Eastern area of the island). In this study, we investigated the levels of genetic diversity and the genetic structure of the species in Sardinia. We used AFLP (amplified fragment length polymorphism) markers to investigate the genetic variability of 182 sampl...

Plants are key elements of wetlands due to their evolutionary strategies for coping with life in a water-saturated environment, providing the basis for supporting nearly all wetland biota and habitat structure for other taxonomic groups. Sardinia, the second largest island of the Mediterranean Basin, hosts a great variety of wetlands, of which 16 are included in eight Ramsar sites. The 119 hydro- and hygrophilous vascular plant taxa from Sardinia represent the 42.6% and 37.9% of the number estimated for Italy and Europe, respectively. Moreover, around 30% of Sardinia’s bryological flora, which is made up of 498 taxa, is present in temporary ponds. An overview at regional scale considering algae is not available, to our knowledge, even though several specific studies have contributed to their knowledge. In order to find the most investigated research themes and wetland types, identify knowledge gaps and suggest recommendations for further research, we present a first attempt to outli...

Understanding seed viability under long-term storage conditions provides basic and useful information to investigate the effectiveness of seed banking. Besides the germination success, seedling establishment is also an important requirement, and a decisive step to ensure plant propagation. We used comparative data of germination, seedling growth, and survival percentage between fresh and 10-years-stored seeds of Senecio morisii, a narrow endemic and vulnerable species of Sardinia (Italy), in order to evaluate if differences exist in these traits between fresh and 10-years-stored seeds. Stored seeds showed higher germination percentages than fresh ones, whereas seedling growth and survival did not present significant differences between them, except for seedling growth in plants produced from seeds germinated at 25 °C. This study allowed us to assess if seeds of S. morisii were able to germinate under controlled conditions, and if they maintained their viability and germination capac...

Background Temperature is the main environmental factor controlling seed germination; it determines both the percentage and the rate of germination. According to the Intergovernmental Panel on Climate Change, the global mean surface temperature could increase of approximately 2–4 °C by 2090–2099. As a consequence of global warming, the period of snow cover is decreasing on several mountain areas. Thermal time approach can be used to characterise the seed germination of plants and to evaluate the germination behaviour under the climate change scenarios. In this study, the effect of different cold stratification periods on seed dormancy release and germination of Gentiana lutea subsp. lutea, a taxon listed in Annex V of the Habitats Directive (92/43/EEC), was evaluated. Furthermore, the thermal requirements and the consequences of the temperature rise for seed germination of this species were estimated. In addition, a conceptual representation of the thermal time approach is presented...

ABSTRACT Knowledge of the levels of genetic diversity and of the spatial genetic structure of plant species is important to ensure their effective management and conservation, especially in the case of endangered species. Gentiana lutea L. subsp. lutea is a long-lived plant which occurs in central and southern European mountains. It has a long-standing history of human exploitation, mainly in the liqueur and in the pharmaceutical industries and it is currently listed in the EU Habitats Directive 92/43/EEC Annex V. Mainly due to a prolonged root harvesting, its current distribution range in Sardinia consists of only a few groups of individuals limited to small areas of the Gennargentu massif (Central-Eastern area of the island). In this study, we investigated the levels of genetic diversity and the genetic structure of the species in Sardinia. We used AFLP (amplified fragment length polymorphism) markers to investigate the genetic variability of 182 samples from 13 subpopulations. A total of 433 fragments were detected, of which 75.5% were polymorphic. The levels of genetic diversity were generally high, but they tended to decrease in smaller subpopulations. Of the genetic variability 88% was found within subpopulations, while the genetic structure among them was fairly weak. In order to ensure the survival of these subpopulations, especially the smaller ones, ex situ and in situ management actions should be planned, such as the long term conservation of its seeds in germplasm repositories and their population reinforcements and monitoring.

Abstract Due to increasing human pressures, there is a need to understand how environmental and anthropogenic disturbances could affect the conservation status of endangered plant species. When information on distribution or population parameters is poor, Species Distribution Models (SDMs) may offer a valuable additional source to assess threats and to evaluate conservation options. In this sense, if the output of SDMs represents the relationships between habitat features and species occurrence, SDM results can also be related to other key parameters of population. For the endangered yellow gentian, we tested the relationship between six field population parameters and the suitabilities obtained by SDMs with natural and limiting parameters (i.e. proxies of disturbances). Specifically, the six population parameters were the surface area covered by each population, the number of vegetative and reproductive individuals per population, the density of reproductive and all individuals per plot and the proportion of reproductive individuals. Thus, threats were evaluated by testing if relationships between population parameters and suitabilities increased when proxies of disturbances were included in models. Best-fitting models resulted when all natural and human disturbance variables were included. In addition, results show relationships between suitability and population parameters only when disturbance parameters were used for SDMs. When the index related to the sensitivity to climate change was included in SDMs, the density of all individuals and number of reproductive plants were lower than in low suitability sites, suggesting that climate change is likely already challenging the ability of yellow gentian to bloom and germinate. Otherwise, we observed a decrease of the extent of localities in areas with higher suitability obtained through the index related to trail intensity. This confirmed the positive effect of conservation activities, which were mainly implemented in the proximity of trails. Using a thoroughly studied plant species as a straightforward example, we showed the potentiality of SDMs to inform on population parameters instead of only discriminate species presence or absence. This information can suggest the use of disturbance parameters when specific SDMs aim to support strategic decisions in management and conservation.

Increases in temperature have been predicted and reported for the Mediterranean mountain ranges due to global warming and this phenomenon is expected to have profound consequences on biodiversity and ecosystem functioning. We hereby present the case of Gentiana lutea L. subsp. lutea, a rhizomatous long-lived plant living in Central-Southern Europe, which is at the edge of its ecological and distributional range in Sardinia. Concretely, we analysed the reproductive success experienced during three phenological cycles (2013/2014, 2014/2015 and 2015/2016) in four representative populations, with particular attention to the phenological cycle of 2014/2015, which has been recorded as one of the warmest periods of the last decades. The Smirnov-Grubbs test was used to evaluate differences in temperature and precipitation regimes among historical data and the analysed years, while the Kruskal-Wallis followed by the Wilcoxon test was used to measure differences between anthesis and reproduct...

SUMMARY Recent extinction rates suggest that humans are now causing the sixth mass extinction, and the Mediterranean islands are at the forefront of many of the environmental issues involved. This study provides an alternative approach for investigating documented local plant extinctions that occurred in Sardinia (western Mediterranean) during the last half century. A total of 190 local extinctions of 62 plant species were used to investigate the independent effects of eight ecological and anthropogenic variables and to model the areas of potential extinctions where plant conservation efforts could be focused. If all analysed plant species were considered together, ecological factors explained local extinctions more than anthropogenic factors. The independent effects of each factor considerably varied among species of different lifeforms and altitude ranges. Accordingly, distribution models of local extinctions outscored areas that are potentially rich in plant species with conserva...

Aims There are a number of mechanisms that regulate germination; among these, seed dormancy, one of the most important, is an adaptative mechanism in plants to promote survival by dispersing germination in space and time until environmental conditions are favourable for germination. The main goals of this study were to determine the temperature requirements for seed dormancy release and germination of Gentiana lutea subsp. lutea, to identify the class and level of seed dormancy and to suggest an optimal germination protocol. Methods Seeds belonging to two different localities were subjected to various pre-treatments, including cold stratification (0 and 5°C), warm stratification (25/10°C) and different combinations of these, and then incubated at a range of constant temperatures (5–25°C) and 25/10°C. Embryo growth during pre-treatments and incubation conditions were assessed at different times by measuring the embryo to seed length ratio (E:S ratio). The final germination percentage (FGP) and the germination rate (t50) were calculated. Important Findings Fleshy mature seeds of G. lutea subsp. lutea have linear underdeveloped embryos. Cold stratification at 0°C was effective in overcoming the physiological dormancy (PD) and promoted embryo growth and subsequent germination. After cold stratification at 0°C, both the root and the shoot emerged readily under a wide range of temperatures. G. lutea subsp. lutea seeds showed an intermediate complex morphophysiological dormancy (MPD). As regards the optimal germination protocol for this taxon, we suggest a period of cold stratification at ca. 0°C followed by seed incubation at 10–20°C. The optimal germination temperatures found for seeds of this taxon, as well as its pre-chilling requirement at 0°C, suggest that it is well adapted to a temperate climate; this behavior highlights an increasing threat from global warming for G. lutea, which could reduce the level of natural emergence in the field, prejudicing also the long-term persistence of the natural populations in Sardinia.

Scientists are typically responsible for greater greenhouse gas emissions than the general population. These 'extra' emissions are largely due to frequent travel, often by airplane, to professional and academic meetings. In the following commentary, we explore how employing mixed modes of transportation, particularly by prioritizing train travel, can significantly reduce the environmental costs associated with attending conferences. Estimating travel distances for attendants to recent meetings, we demonstrate that the proposed strategy has the potential to decrease emissions, even when considering exotic, remote (and potentially enticing) locations. Our suggestions are easy to implement, can be combined with previous proposals for reducing emissions, and provide an opportunity for scientists to convey an important message to the general public. This message is not confined to our particular example and we hope that it will encourage ecologists, sociologists and other scienti...

Abstract Regional assessments could be more reliable than global ones, especially for species in peripheral parts of their range. In this sense, distribution criteria proposed by the IUCN, expressed by the Extent of Occurrence (EOO) and the Area of Occupancy (AOO), are of critical importance to evaluate the risk category at any scale. Our study was focused on the yellow gentian ( Gentiana lutea L. subsp. lutea ), a rhizomatous mountain plant of central and southern Europe, included in the Annex V of the Directive 92/43/EEC. The exhaustive and updated regional distribution and the recently published extinct localities, allowed for review of option to increase the number of criteria used for its regional conservation status assessment. In particular, the current restricted EOO and AOO extents and reductions occurred during the last three generations led this plant being considered as Endangered (EN), according to the IUCN sub-criteria A2, B1 and B2. Additionally, Species Distribution Models based on the extant localities and the respective current and future climate scenarios (2050 and 2070) predicted a decrease of its environmental niche reducing its altitudinal range towards higher elevations. This reduction was greater than 50% and confirmed the regional conservation status of EN, also according to the sub-criterion A3. This paper shows the reliability of regional risk assessment of peripheral and/or narrow populations, which is an effective tool for helping the development of the necessary conservation management, underling a diversified set of solutions for each specific past, present and future threat that could occur in each locality.

ABSTRACT Species Distribution Models (SDMs) could be even an important tool to limit search efforts by selecting the areas where field surveys are to be carried out; due to the constant decrease of financial funds, this challenging purpose is particularly necessitous. In particular, these methods are useful when applied to endangered and/or rare species with a poor known distribution area, especially due to difficulties in plant detection and in reaching the study areas.