Anongnart Assavacheep

The effects of -glucan and mannan-oligosaccharide (BG + MOS) supplementation in feed on growth performance, bacterial population in feces and immune responses of weaned pigs were investigated. Two hundred and eighty-eight weaning piglets were randomly allocated into 3 groups with 8 replicates per group and 12 pigs per replicate. Group 1, as control, was fed a commercial basal nursery diet. Groups 2 and 3 were fed the same diet supplemented with BG + MOS (Biolex® MB-40) 1 and 2 kg/ton of feed, respectively. Weight gain (WG), average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) were calculated on days 1, 21 and 42 of experiment. Blood samples were analyzed for interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNF-). Total bacteria, total coliform, Lactobacillus concentrations and Lactobacillus to Coliform ratio (L:C) in feces were also determined. Results showed that groups 2 and 3 had better WG, ADFI and ADG on days 1-21. No significant...

Objective Gut health improvements were monitored with respect to growth performance, diarrhea incidence, fecal bacterial population and intestinal morphology of suckling pigs orally supplemented with live Lactobacillus salivarius (L. salivarius) oral suspensions and challenged with F4+ enterotoxigenic Escherichia coli (ETEC). Methods Two groups of newborn pigs from 18 multiparous sows were randomly designated as non-supplemented (control: n = 114 piglets) and L. salivarius supplemented groups (treatment: n = 87 piglets). Treatment pigs were orally administered with 2 mL of 109 colony-forming unit (CFU)/mL L. salivarius on days 1 to 3, then they were orally administered with 5 mL of 109 CFU/mL L. salivarius on days 4 to 10, while those in control group received an equal amount of phosphate buffered saline solution. On day 24 (2 weeks post supplementation), one pig per replicate of both groups was orally administered with 108 CFU/mL F4+ ETEC, then they were euthanized on day 29 of exp...

The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs for the presence of Haemophilus parasuis and predict the virulence of the strains present. Nasal cavity swabs were taken from 30 live, healthy, 1- to 8-week-old pigs on a weekly cycle from a commercial Thai nursery pig herd. A total of 27 of these pigs (90%) tested positive for H. parasuis as early as week 1 of age. None of the H. parasuis-positive samples from healthy pigs was positive for the hhdBA genes. At the same pig nursery, swab samples from nasal cavity, tonsil, trachea, and lung, and exudate samples from pleural/peritoneal cavity were taken from 30 dead pigs displaying typical pathological lesions consistent with Glässer disease. Twenty-two of 140 samples (15.7%) taken from 30 diseased pigs yielded a positive result for H. parasuis. Samples from the exudate (27%) yielded the mos...

In sheep, follicle populations are very responsive to dietary intake. Definitions of effect of nutrition on ovulation rate have been identified 1) static effect, 2) dynamic effect and 3) immediate effect. An example of the immediate effect is short-term lupin grain supplementation for 4-6 days during the end of luteal phase prior to ovulation. This treatment increases the number of follicles and ovulation rate without altering body weight. The mechanisms of nutritional effects on folliculogenesis are not effects associated with the amount of nutrient supplies. It is much more prone that they are specific nutrient signaling effects. Nutrition modulates reproductive function at multiple levels through the circulating metabolic hormones such as insulin, insulin-like growth factor-I and growth hormone. These hormones have important roles in the control of follicle growth and are likely to be mediators of the effects of nutritional intake on ovulation rate, by acting to regulate the acti...