Through the use of assisted reproductive technology (ART), we will be able to extend the use of genetically superior animals, and perhaps increase the number of offspring. These technologies include such procedures as estrus... more
Through the use of assisted reproductive technology (ART), we will be able to extend the use of genetically superior animals, and perhaps increase the number of offspring. These technologies include such procedures as estrus synchronization, superovulation, artificial insemination, embryo transfer, in vitro fertilization (IVF), and cloning. Because they exhibit seasonal breeding and multiple ovulations, sheep have a tremendous potential for improvement and manipulation of reproduction with the use of ART. Most sheep normally exhibit estrous cycles and mate during late summer, fall, and early winter. During winter, spring, and early summer they exhibit anestrous, and thus, are reproductively inactive. Reproductive performance of sheep, in some cases, can be maximized by utilizing this anestrous period or non-breeding season. Manipulations to maximize reproductive performances during seasonal anestrous include hormonal stimulations and estrous synchronization. However, many improvements are still needed to enhance the reproductive efficiency of ewes during seasonal anestrous.
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Supplementation of organic trace mineral (OTM) may affect reproductive functions in several aspects and has been studied, but very rare is reported in dairy cows. To determine the effects of dietary OTM supplement during pre- and... more
Supplementation of organic trace mineral (OTM) may affect reproductive functions in several aspects and has been studied, but very rare is reported in dairy cows. To determine the effects of dietary OTM supplement during pre- and postpartum with timed artificial insemination (TAI) protocol on steroid hormones, antioxidant enzymes, and reproductive performances in dairy cows. Prepartum Holstein cows (n = 60) were randomly assigned to received either a control diet or OTM supplemented for 42 days. Both groups were fed the same total mixed ration, but were supplemented with 5 g/head/d OTM (Bioplex®) in the treatment group. Blood and follicular fluid samples were collected to determine progesterone (P4), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and estradiol (E2) concentrations. Cows fed OTM had better placental expulsion period, milk yield, and reproductive performance parameters (day to first ovulation and estrus, percentage of first estrus and size of preovulatory...
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The objective of this study was to investigate the effect of mangosteen peel and garlic powder supplementation on the intake, digestibility, ruminal fermentation and methane production in beef cattle steers fed on rice straw base of diet.... more
The objective of this study was to investigate the effect of mangosteen peel and garlic powder supplementation on the intake, digestibility, ruminal fermentation and methane production in beef cattle steers fed on rice straw base of diet. Four, 2 year old rumen fistulated male crossbred beef cattle were randomly assigned in a 4x4 Latin square design to receive four dietary treatment; mangosteen peel pellet (Mago-pel), mangosteen peel with garlic powder pellet (Maga-Lic), mangosteen peel pellet with urea and garlic powder (Maga-ulic) 200g/h/d and non-supplemented (control). All animals were fed with rice straw adlibitum while of addition concentrate was fed 0.5 % body weight (BW). It was found that supplementation of mangosteen peel and garlic with or without urea had no effect on daily feed intake (FI), digestibility of dry matter (DM), organic matter (OM) and crude protein (CP) and was not significantly affected. Supplementation resulted in significantly lower overall NDF and ADF d...
The aim of the study was to compare the efficiency of first use and re-use synthetic progesterone on estrus synchronization and pregnancy rate after natural breeding and Timed artificial insemination (TAI) in goats. Experiment 1, goats... more
The aim of the study was to compare the efficiency of first use and re-use synthetic progesterone on estrus synchronization and pregnancy rate after natural breeding and Timed artificial insemination (TAI) in goats. Experiment 1, goats (n=28) with the average age and body weight of 10 months and 17 kg received 14 days of controlled internal drug release (CIDR) device (the first use; Eazi-BreedCIDR) or the re-use of CIDR device. All goats were given 300 IU injection of hCG (Chorulon) at the day of CIDR withdrawal. No differences in percentage of estrus and ovulation were observed. However goats, received the re-use of CIDR device, exhibited shorter (P<0.05) duration of estrus (21.3 h) compared to goats in the first use (27.0 h) and delayed estrus and time of ovulation (70.8 and 93.3 h) compared to the first use of CIDR (38.5 and 67.0 h). Plasma progesterone (P4) concentrations were significantly different (P<0.05) between treatments during CIDR device insertion and after CIDR w...
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The aim of the study was to investigate the relation among mitotic activity, total protein concentrations, and amount of bovine follicular fluid IGFBP-4 on day 5 of the first follicular wave. Eight mature Thai native crossbred beef cows... more
The aim of the study was to investigate the relation among mitotic activity, total protein concentrations, and amount of bovine follicular fluid IGFBP-4 on day 5 of the first follicular wave. Eight mature Thai native crossbred beef cows received two injections (11 days apart) of a PGF2α analogue (25 mg of Estrumate®) to synchronize estrus and ovaries were 1 ภาควิชาสัตวศาสตร คณะเกษตรศาสตร มหาวิทยาลัยขอนแกน ขอนแกน 40002 Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand 40002 2 ศูนยวิจัยเทคโนโลยีชีวภาพทางการเกษตรเพ่ือเศรษฐกิจที่ย่ังยืน มหาวิทยาลัยขอนแกน ขอนแกน 40002 The Agricultural Biotechnology Research Center for Sustainable Economy, Khon Kaen University 40002 มหาวิทยาลัยเทคโนโลยีราชมงคลอีสาน วิทยาเขตกาฬสินธุ กาฬสินธุ 46000 Rajamangala University of Technology Isan Kalasin Campus, Thailand 46000 *Corresponding author E-mail address: chanav@kku.ac.th collected from a slaughterhouse on day 5 of the subsequent estrous cycle. Follic...
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To determine the effects of Follicle Stimulating Hormone (FSH) treatment and subsequent withdrawal on uterine proliferation and estrogen receptor (ESR), Brahman crossbred heifers (n = 12) were twice daily injected with FSH (4, 3, and... more
To determine the effects of Follicle Stimulating Hormone (FSH) treatment and subsequent withdrawal on uterine proliferation and estrogen receptor (ESR), Brahman crossbred heifers (n = 12) were twice daily injected with FSH (4, 3, and 2 mg/injection) on Days 17–19 of the estrous cycle (FSH 3 days) and (4 and 3 mg/injection) on Days 17–18 (FSH 2 days) and withdrawal with saline on Day 19 and (4 mg/injection) on Day 17 (FSH 1 day) and withdrawal with saline on Days 18–19. Uterine tissue was subjectively collected on Day 20 and microscopically classified to four regions: endometrial stroma (ES), surface endometrial gland (EG), deep endometrial gland (DG), and myometrium (Myo). The cell proliferation marker, Ki‐67, was quantified as labeling index (LI) in uterine regions, and tissues were immunostained to detect ESR2 followed by image analysis. The LI of ES, EG, and DG was greater (P = 0.0018, P = 0.0005, and P = 0.0103; respectively) in heifers received FSH for 3 days. The expression of...
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To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1α) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU... more
To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1α) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1α were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P< 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1α at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8–24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1α in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relatio...
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Administration of FSH increases the number of developing follicles, and affects oocyte health and cleavage rate. To determine the optimal level of FSH treatment, studies were conducted during the normal breeding season and seasonal... more
Administration of FSH increases the number of developing follicles, and affects oocyte health and cleavage rate. To determine the optimal level of FSH treatment, studies were conducted during the normal breeding season and seasonal anestrus. In Experiment 1, ewes were implanted with SyncroMate-B (SMB; norgestomet) for 14 days during the breeding season. Beginning on day 12 or 13 after SMB implantation, ewes were treated with saline (control; n=10), or treated with FSH for two days (2D; n=9) or three days (3D; n=10). In Experiment 2, conducted during seasonal anestrus, ewes were implanted with SMB for 14 days (n=23) or were not implanted (n=26). The SMB-implanted and nonimplanted ewes were assigned to one of three treatments as in Experiment 1: control (n=13), 2D (n=21) or 3D (n=15). In Experiments 1 and 2, ewes were laparotomized to count the number of follicles &lt; or = 3 mm and &gt; 3 mm and to retrieve oocytes. Healthy oocytes from each treatment were used for IVF. In Experiment 3, ewes (n=6) were implanted twice with SMB for 14 days during seasonal anestrus. Ewes were injected with FSH for 2 days, and the oocytes were collected and fertilized as in Experiments 1 and 2. In Experiment 1, FSH-treatment increased (P &lt; 0.05) the number of follicles &gt; 3 mm, the number of oocytes retrieved from follicles &lt; or = 3 mm and &gt; 3 mm, the proportion of healthy oocytes, and the number of oocytes used for IVF. Oocytes from control and 2D ewes had greater (P &lt; 0.01) cleavage rates than 3D ewes (68% and 71% vs. 42%). In Experiment 2, implanted and nonimplanted ewes had similar (P &gt; 0.05) numbers of follicles, total oocytes, and healthy oocytes; therefore, data were combined. The FSH treatment increased (P &lt; 0.01) the number of follicles &gt; 3 mm, and the number of oocytes recovered from follicles &gt; 3 mm. The recovery rate of oocytes and the percentage of healthy oocytes were similar for control and FSH-treated ewes. The cleavage rate in Experiment 2 ranged from 4 to 16%. In Experiment 3, the cleavage rate for ewes treated twice with SMB was 27% which tended to be greater (P &lt; 0.07) than for the 2D ewes that received one SMB implant in Experiment 2. These data indicate that FSH increased the number of developing follicles and the number of healthy oocytes retrieved from ewes during the breeding season and seasonal anestrus. However, cleavage rates during seasonal anestrus were lower than during the normal breeding season in both FSH-treated and control ewes. Treatment of ewes for 2 days with FSH resulted in a greater cleavage rate than treatment of ewes for 3 days.
Research Interests: Biology, Medicine, Biological Sciences, Pregnancy, Female, and 15 moreAnimals, In Vitro Fertilization, Male, Ovarian Follicle, Fertilization in Vitro, Elsevier, Oocytes, Oocyte, Follicle stimulating hormone, Estrous cycle, Breeding season, Random Allocation, Follicular phase, Medical and Health Sciences, and Ovarian follicles
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To determine the relationships among vascularity, expression of angiogenic factors, and selected intrafollicular factors in dominant and nondominant follicles of the first follicular wave, ovaries were obtained on d 3 of the estrous cycle... more
To determine the relationships among vascularity, expression of angiogenic factors, and selected intrafollicular factors in dominant and nondominant follicles of the first follicular wave, ovaries were obtained on d 3 of the estrous cycle from mature cross-bred beef heifers (n = 8) after a synchronized estrus. Follicular fluid (FF) was collected from all follicles &gt; or = 3 mm for determination of estradiol-17beta (E), progesterone (P4), vascular endothelial growth factor (VEGF), and IGFBP concentrations. The ovaries were then perfusion-fixed and used for histochemical detection of lectin BS-1 (a marker of endothelial cells and thus vascularization) binding, and immunolocalization of VEGF, endothelial nitric oxide synthase (eNOS), and proliferating cell nuclear antigen, followed by image analysis of selected follicles. Follicles were classified, based on E and P4 concentrations in FF, as dominant, estrogen-active (EA; E:P4 &gt; or = 1) or nondominant, estrogen-inactive (EI; E:P4 &lt;1). Concentrations of E and VEGF in FF, the area of positive staining for lectin BS-1, VEGF, and eNOS, and the labeling index (an index of the percentage of cells proliferating) in granulosa and theca layers were greater (P &lt; 0.05) in the EA than in the EI follicles, but concentrations of P4 and IGFBP in FF were less (P &lt; 0.05) in EA than in EI follicles. In addition, vascularity was positively correlated (P &lt; 0.05) with VEGF and eNOS protein expression, and tended (P &lt; 0.1) to be positively correlated with the E:P4 ratio in FF but tended (P &lt; 0.1) to be negatively correlated with IGFBP and P4 concentrations in FF. These data highlight the importance of vascularity, angiogenic factors, and IGFBP in the health of the dominant follicle in heifers, and indicate that the FF concentrations of E, VEGF, IGFBP, and P4, and the E:P4 ratio can be used as markers of dominant follicles.
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The aim of the study was to determine the quantitative vascularity of the bovine preovula- tory follicle using Factor VIII immunolocalization associated with the follicular health. Eight mature Thai native crossbred beef cows received two... more
The aim of the study was to determine the quantitative vascularity of the bovine preovula- tory follicle using Factor VIII immunolocalization associated with the follicular health. Eight mature Thai native crossbred beef cows received two injections (11 day apart) of a PGF analogue to synchronize estrus. The study was conducted to test the marker of endothelial cell (Factor VIII) and granulosa cell (Pyknotic nucleus) for follicular health evaluation. Bovine preovulatory follicles (n=32) were obtained on day 6 of the estrous cycle from abattoir located in Khon Kaen provine. Follicles were classified by size as 3-6, 7-10, and >10 mm in diameter and by morpho- logy as healthy and unhealthy. Ovarian tissue samples were fixed and paraffin-embedded prior immunohistochemistry. Factor VIII (Sigma, USA) and Pyknotic nuclei were applied as marker of endothelial cell in the theca and granulosa cell respectively. Quantitative and qualitative analysis of follicular health was determined with ...
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The aim of the experiment was to evaluate the effects of GnRH and/or eCG and progestin type (implant versus CIDR) on the induction of estrus and pregnancy rate following laparoscopic AI (LAI) with frozen semen. In the first trial, ewes... more
The aim of the experiment was to evaluate the effects of GnRH and/or eCG and progestin type (implant versus CIDR) on the induction of estrus and pregnancy rate following laparoscopic AI (LAI) with frozen semen. In the first trial, ewes (n=129) were treated with norgestomet implants for 14 days. At implant removal ewes received eCG (400IU) and/or GnRH (25μg) 36h
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The objective of this experiment was to determine the effect of culture media (Tyrode Albumin Lactate Pyruvate-In Vitro Fertilization-Synthetic Oviduct Fluid; TALP- IVF-SOF and SAGE media TM ) on fertilization rate in bovine and caprine... more
The objective of this experiment was to determine the effect of culture media (Tyrode Albumin Lactate Pyruvate-In Vitro Fertilization-Synthetic Oviduct Fluid; TALP- IVF-SOF and SAGE media TM ) on fertilization rate in bovine and caprine embryos produc- tion. Thai crossbred bovine ovaries (n=40) were collected at a local abattoir located in Khon Kaen municipality. Caprine ovaries (n=22) were collected by the surgery method at the De- partment of Animal Science, Faculty of Agriculture, Khon Kaen University. In order to compare the culture media, oocytes were recovered and randomly cultured in TALP-IVF- SOF and SAGE media TM during in vitro oocytes maturation and fertilization as described by the standard protocols for in vitro maturation, in vitro fertilization and in vitro culture, re- spectively. Oocytes, obtained from ovaries with corpus luteum (CL) and without CL, were recovered and determined as recovery rate prior in vitro culture. The results revealed that the recovery rates of...
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This research aimed to evaluate the effects of anthocyanin-extracted residue (AER) in the diet of cattle on meat oxidation during storage and on the fatty acid profiles of the meat. Sixteen male dairy cattle (average body weight 160 ±... more
This research aimed to evaluate the effects of anthocyanin-extracted residue (AER) in the diet of cattle on meat oxidation during storage and on the fatty acid profiles of the meat. Sixteen male dairy cattle (average body weight 160 ± 10.6 kg) were allotted to feed in a completely randomized design (CRD) with four levels of AER supplementation, 0, 20, 40, and 60 g/kg dry matter (DM) in the total mixed ration (TMR). These TMR diets were fed ad libitum to the cattle throughout the trial. At the end of the feeding trial (125 days), all cattle were slaughtered and meat samples from the Longissimus dorsi (LD) muscle were collected to assess meat oxidation and fatty acid profile. The antioxidant effect of AER on meat oxidation was investigated during 14 days of storage based on color, myoglobin redox forms, lipid, and protein oxidation. The results showed meat from cattle fed AER had better color stability, lower oxidation of lipid, protein and myoglobin than did meat from cattle fed the ...
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Accumulation of lipid droplets (LD) in luteal cells likely is important for energy storage and steroidogenesis in the highly metabolically active corpus luteum (CL). The objective of this study was to determine the effect of plane of... more
Accumulation of lipid droplets (LD) in luteal cells likely is important for energy storage and steroidogenesis in the highly metabolically active corpus luteum (CL). The objective of this study was to determine the effect of plane of nutrition on progesterone (P4) secretion, and lipid droplet number and size in cultured ovine luteal cells. Ewes were randomly assigned to one of three nutritional groups: control (C; 100% NRC requirements, n=9), overfed (O; 2×C, n=12), or underfed (U; 0.6×C, n=10). Superovulation was induced by follicle stimulating hormone injections. At the early and mid-luteal phases of the estrous cycle, CL were dissected from ovaries, and luteal cells isolated enzymatically. Luteal cells were incubated overnight in medium containing serum in chamber slides. Media were then changed to serum-free and after 24h incubation, media were collected for P4 analysis, and cells were fixed in formalin and stained with BODIPY followed by DAPI staining. Z-stacks of optical secti...
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Research Interests: Animal Science, Dairy Science, Fertility, Lactation, Medicine, and 15 moreAnimal Production, Pregnancy, Female, Ovulation Induction, Animals, Body Composition, Dairy, Cattle, Artificial insemination, Gonadotropin Releasing Hormone, Food Sciences, Body Condition Score, Insemination, Estrous cycle, and Estrus Synchronization
Objective—The present study was conducted to determine the fetal and maternal growth during early pregnancy in Thai-native goats. Materials and Methods—Sixteen Thai native goats with the average age and weight of 24.7±2.9 months and... more
Objective—The present study was conducted to determine the fetal and maternal growth during early pregnancy in Thai-native goats. Materials and Methods—Sixteen Thai native goats with the average age and weight of 24.7±2.9 months and 23.8±1.6 kg were used. Does were synchronized estrus using medroxyprogesterone acetate and natural mated with a fertile goat. Does were pregnancy diagnosed using ultrasonography, weighed and examined crown-rump length every week post mating starting from day 21-56 of gestation. Results—The average early pregnancy diagnosis was 25.0±1.4 days. Fetal growth equations as de-termined by the crown-rump length for single (Y 1), twin (Y 2) and triplet (Y 3) pregnancies were Y 1 = 2.934e 0.570X (r 2 = 0.945), Y 2 = 3.444e 0.564X (r 2 = 0.986) and Y 3 = 8.161e 0.320X (r 2 = 0.917), respectively. Ma-ternal growth of single, twin and triplet pregnancy was increased gradually with the linear equation of Y 1 = 21.5+0.516X (r 2 = 0.993), Y 2 = 24.43+0.717X (r 2 = 0.986...
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Objective—The study was conducted to determine the effect of serum types on Interferon-Tau (IFN-t) and BCL-2 expression in bovine embryos production. Materials and Methods—Bovine ovaries (n=54) were obtained at a local slaughterhouse.... more
Objective—The study was conducted to determine the effect of serum types on Interferon-Tau (IFN-t) and BCL-2 expression in bovine embryos production. Materials and Methods—Bovine ovaries (n=54) were obtained at a local slaughterhouse. Oocytes were cultured in TCM-199 supplemented with serum type as treatments (10% BSA, 10% FCS, and 10% ECS, respectively). Mature oocytes were placed in Fert-TALP media for fertilization. Total cellular RNA sam-ples were extracted from frozen embryos. RNA samples were used for cDNA synthesis using a reverse transcription. A relative abundance mRNA of IFN-t and BCL-2 was determined by a quantitative real-time RT-PCR. Results—The recovery rates of oocytes and the percentages of healthy oocytes were significantly greater in 3-8 mm follicles than in <3 mm follicles (P<0.01). Percentages of mature oocytes in TCM-199 supple-mented with 10% FCS, ECS and BSA were not significantly different (P>0.05) compared to the control. Percentages of fertilized e...
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This study was conducted to determine the growth and development of corpora lutea (CL) in Thai-native goats throughout the estrous cycle. Non pregnant Thai-native goats (n=12), 8 months of age were randomly assigned for estrous... more
This study was conducted to determine the growth and development of corpora lutea (CL) in Thai-native goats throughout the estrous cycle. Non pregnant Thai-native goats (n=12), 8 months of age were randomly assigned for estrous synchronization and superovulation protocols (progesterone 14 days+PMSG vs. progesterone 14 days+FSH). After progesterone removal, goats underwent laparotomy to determine number of CL post ovulation at 24, 48, 72 and 96 h. Blood sample were taken for progesterone concentration throughout the estrous cycle. Number of CL in goats receiving the protocols were not significantly different at times after ovulation (P>0.05) except for at 24 h (P=0.04). Concentration of plasma progesterone (P4) were not significantly different among goats difference in CL numbers (1, 2 and 3), with the concentration of 6.36, 5.45 and 5.72 ng/ml respectively. Plasma P4 concentrations of goats were low (<1 ng/ml) during the first 5 days of the estrous cycle, but significantly inc...
The aim of the study was to compare the efficiency of short-term and long-term synthetic progesterone on estrous synchronization and conception rate in Thai-native goat. Two experiments were conducted to meet the objectives of the... more
The aim of the study was to compare the efficiency of short-term and long-term synthetic progesterone on estrous synchronization and conception rate in Thai-native goat. Two experiments were conducted to meet the objectives of the research as follows. Experiments 1, Thai-native goats (8-10 months of age) were randomly from the herd with the average weight of 16.9 kg. The goats were randomly assigned into two groups different treatment as follow: Treatment 1 goats received intravaginally controlled internal drug release (CIDR) device 0.3 g of progesterone for 14 days (long-term protocol) with the combination 0.5 ml PGF2α and 150 IU PMSG or Treatment 2 goats received intravaginally CIDR for 5 days (short-term protocol) with the same combination of PGF2α and PMSG. Plasma progesterone concentrations after CIDR removal at 0-96 h in goats received long-term protocol significantly lower than goats received short-term protocol indicating that goats responded for long-term protocol than shor...
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A study was conducted during hot season to determine the effect of synchronization of ovulation with human chorionic gonadotropin (hCG) on fertility of lactating dairy cows with ovarian cysts. Non cyclic Holstein dairy cows (n = 80) were... more
A study was conducted during hot season to determine the effect of synchronization of ovulation with human chorionic gonadotropin (hCG) on fertility of lactating dairy cows with ovarian cysts. Non cyclic Holstein dairy cows (n = 80) were stratified by parity and diagnosed as having an ovarian cyst. The cows were further identified as follicular or luteal cysts according to the plasma progesterone (P4) concentration and the cystic image of ultrasonography. Cystic cows were randomly assigned to receive treatments (Ovsynch as the control or Ovsynch plus 3000 IU hCG). All cows were artificially inseminated at 16-18 h after the second gonadotropin releasing hormone injection. Cows supplemented with hCG had a greater number of corpus luteum (1.8 ± 0.2 and 0.8 ± 0.3; P < 0.05) and had greater P4 concentration on day 12 than those control cows (6.3 ± 0.3 and 3.9 ± 0.4 ng/ml; P < 0.05). Concentration of cortisol did not differ between groups of cystic cows. No significant differences w...
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The study was conducted to compare estrous rate, ovulatory response, plasma progesterone concentrations, and conception rate following cervical artificial insemination in goats given a new or once-used controlled internal drug release... more
The study was conducted to compare estrous rate, ovulatory response, plasma progesterone concentrations, and conception rate following cervical artificial insemination in goats given a new or once-used controlled internal drug release (CIDR) device with human chorionic gonadotropin (hCG). Fifty-six Thai-native goats with the average age and body weight of 11 months and 17.3 kg received a 14-day treatment with a new CIDR device (Eazi-Breed(TM)CIDR®, Pfizer, NY, USA) or a once-used CIDR device. All goats received a 300-IU injection of hCG (Chorulon®, Intervet International B.V., New Zealand) at the day of CIDR removal to induce ovulation. All goats displaying signs of Estrous behavior were artificially inseminated at 12 h after the onset of estrus with frozen semen. No differences in percentage of estrus and ovulation rates were observed; however, goats that received once-used CIDR devices exhibited shorter (P < 0.05) duration of estrus in comparison with new devices (21.4 ± 1.4 h ...
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Research Interests: Endocrinology, Medicine, Biological Sciences, Melatonin, Progesterone, and 15 moreInternal Medicine, Female, Animals, In Vitro Fertilization, Drug Delivery Systems, Ovarian Follicle, Fertility Rate, Fertilization in Vitro, Morning, Oocytes, Follicle stimulating hormone, Estrous cycle, Follicular phase, Adverse effect, and Medical and Health Sciences
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Utero-placental growth and vascular development are critical for pregnancy establishment that may be altered by various factors including assisted reproductive technologies (ART), nutrition, or others, leading to compromised pregnancy. We... more
Utero-placental growth and vascular development are critical for pregnancy establishment that may be altered by various factors including assisted reproductive technologies (ART), nutrition, or others, leading to compromised pregnancy. We hypothesized that placental vascularization and expression of angiogenic factors are altered early in pregnancies after transfer of embryos created using selected ART methods. Pregnancies were achieved through natural mating (NAT), or transfer of embryos from NAT (NAT-ET), or IVF orin vitroactivation (IVA). Placental tissues were collected on day 22 of pregnancy. In maternal caruncles (CAR), vascular cell proliferation was less (P<0.05) for IVA than other groups. Compared with NAT, density of blood vessels was less (P<0.05) for IVF and IVA in fetal membranes (FM) and for NAT-ET, IVF, and IVA in CAR. In FM, mRNA expression was decreased (P<0.01–0.08) in NAT-ET, IVF, and IVA compared with NAT for vascular endothelial growth factor (VEGF) and...
Research Interests: Physiology, Andrology, Reproduction, Animal Production, Pregnancy, and 13 morePlacenta, Sheep, Female, Animals, Embryo Transfer, Behavioral Animal Models, Clinical Sciences, Time Factors, Fertilization in Vitro, Veterinary Sciences, Vascular Endothelial Growth Factor, Paediatrics and reproductive medicine, and placental growth factor
ABSTRACT To quantify vascularity of antral follicles in Bos indicus using Factor VIII immunohistochemistry in order to investigate the relationship between follicular size, health status and vascularity, bovine ovaries were obtained on d... more
ABSTRACT To quantify vascularity of antral follicles in Bos indicus using Factor VIII immunohistochemistry in order to investigate the relationship between follicular size, health status and vascularity, bovine ovaries were obtained on d 6 of the estrous cycle from mature B. indicus (n=8) after a synchronized estrus with PGF(2 alpha) analogue. The ovaries were perfused and used for immunofluorescence detection of Factor VIII (a marker of endothelial cells). Follicles were classified by size (small, medium, and large) and by morphology as healthy and atretic follicle. Quantitative analysis was determined by the aid of image analysis. Factor VIII binding was localized exclusively in the thecal layer and stroma of the antral follicles, but was not found in the granulosa layer. For all follicles evaluated, capillary area density (CAD) and capillary number density (CND) were greater (P&lt;0.05) in the small and medium follicles compared to the large follicle. However, area per capillary (APC) was greatest in small follicles compared with large follicles but was not different when compared with medium follicles. The average CAD, CND, and APC of the healthy follicles were greater (P &lt;0.05) than that of the atretic follicles. The CAD was also greater (P&lt;0.05) in the healthy follicle (small, medium, and large) compared to the atretic follicle (small, medium, and large). CND was greatest (P &lt; 0.05) in theca layer of healthy small follicle and was lowest in atretic large follicle. APC was least (P &lt;0.05) in theca layer of the atretic large follicle. These results demonstrated that immunofluorescence detection of Factor VIII can be used as a reliable marker of endothelium to quantify vascularity of antral follicles in B. indicus. There was no interaction between follicular health and size on vascularity of antral follicle. A decline in vascularity was observed in atretic follicle and large size of atretic follicle in B. indicus.