Laboratory technologies of synthesis of composite nanoparticles containing plasmon resonant core ... more Laboratory technologies of synthesis of composite nanoparticles containing plasmon resonant core (gold nanospheres, nanoshells, nanorods and gold-silver nanocages) and silica shell doped with photosensitizer with tuning of optical properties in visible and infrared regions were developed. Composite nanoparticles were utilized for effective combined photothermal and photodynamic inactivation of pathogenic bacteria and grafted tumors in rats. New types of nanomaterials – powders of gold and gold-silver nanoparticles easy soluble in water media without any additional manipulations and maintaining all properties of parent colloids were synthesized. The nanopowders were investigated with in vitro and in vivo experimental models for the first time. The nanoparticle powders can be stored for a long time under usual conditions which significantly simplifies the transfer of laboratory technologies to clinics. In particular, of the gold nanorod powders were used for photothermolysis of xenogr...
ABSTRACT The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the... more ABSTRACT The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.
A wet-chemical approach is used to fabricate centimeter-scale gold nanoisland films (NIFs) with t... more A wet-chemical approach is used to fabricate centimeter-scale gold nanoisland films (NIFs) with tunable morphology of islands and with strong electromagnetic coupling between them. The approach consists in a uniform seeding of small gold nanoparticles on a glass or silicon substrate, followed by controllable growth of the seeds into small nanoislands. A special technique for TEM sampling was developed to follow the gradual formation of larger-sized isolated nanoparticles, nanoislands of sintered overgrown seeds, and a complete gold layer with nanoscale cracks. The electromagnetic field distribution inside the fabricated NIFs was calculated by FDTD simulations applied to actual TEM images of the fabricated samples rather than to artificial models commonly used. SERS measurements with 1,4-aminothiophenol (ATP) molecules demonstrated the analytical enhancement factor about of 10(7) and the fundamental enhancement factor about of 10(8) for optimized substrates. These values were at leas...
This paper presents a comparative analysis of the combined and separate influence of ultrasound a... more This paper presents a comparative analysis of the combined and separate influence of ultrasound and DMSO on the transport of a gold nanoshell suspension in intact and injured skin from data on optical coherence tomography and histochemical analysis. Experimental allergic contact dermatitis was used to model injury to the stratum corneum during various pathological changes in the skin. The studies were per� formed on outbred laboratory rats. It is shown that the best method for enhancing transdermal transport of an immersion liquid is multimodal physical and chemical impact (a combination of DMSO and ultrasono� phoresis); the effectiveness of optical clearing of the dermis both in the presence and absence of the stratum corneum is approximately the same. To enhance the transport of nanoparticles into the skin when it under� goes pathological changes related to injuries of the protective barrier, exposure to ultrasound is sufficient.
The static and dynamic light scattering (DLS) methods were applied to study the thermodynamic pro... more The static and dynamic light scattering (DLS) methods were applied to study the thermodynamic properties and disperse structure of water+surfactant+solubilizer systems. It is supposed that such systems possess a micellar structure that can be used to develop new effective forms of veterinary drugs. For preparation of drugs (antibiotics) in micellar form, water solutions of the surfactant Cremafor-EL (CR) with the
Laboratory technologies of synthesis of composite nanoparticles containing plasmon resonant core ... more Laboratory technologies of synthesis of composite nanoparticles containing plasmon resonant core (gold nanospheres, nanoshells, nanorods and gold-silver nanocages) and silica shell doped with photosensitizer with tuning of optical properties in visible and infrared regions were developed. Composite nanoparticles were utilized for effective combined photothermal and photodynamic inactivation of pathogenic bacteria and grafted tumors in rats. New types of nanomaterials – powders of gold and gold-silver nanoparticles easy soluble in water media without any additional manipulations and maintaining all properties of parent colloids were synthesized. The nanopowders were investigated with in vitro and in vivo experimental models for the first time. The nanoparticle powders can be stored for a long time under usual conditions which significantly simplifies the transfer of laboratory technologies to clinics. In particular, of the gold nanorod powders were used for photothermolysis of xenogr...
ABSTRACT The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the... more ABSTRACT The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.
A wet-chemical approach is used to fabricate centimeter-scale gold nanoisland films (NIFs) with t... more A wet-chemical approach is used to fabricate centimeter-scale gold nanoisland films (NIFs) with tunable morphology of islands and with strong electromagnetic coupling between them. The approach consists in a uniform seeding of small gold nanoparticles on a glass or silicon substrate, followed by controllable growth of the seeds into small nanoislands. A special technique for TEM sampling was developed to follow the gradual formation of larger-sized isolated nanoparticles, nanoislands of sintered overgrown seeds, and a complete gold layer with nanoscale cracks. The electromagnetic field distribution inside the fabricated NIFs was calculated by FDTD simulations applied to actual TEM images of the fabricated samples rather than to artificial models commonly used. SERS measurements with 1,4-aminothiophenol (ATP) molecules demonstrated the analytical enhancement factor about of 10(7) and the fundamental enhancement factor about of 10(8) for optimized substrates. These values were at leas...
This paper presents a comparative analysis of the combined and separate influence of ultrasound a... more This paper presents a comparative analysis of the combined and separate influence of ultrasound and DMSO on the transport of a gold nanoshell suspension in intact and injured skin from data on optical coherence tomography and histochemical analysis. Experimental allergic contact dermatitis was used to model injury to the stratum corneum during various pathological changes in the skin. The studies were per� formed on outbred laboratory rats. It is shown that the best method for enhancing transdermal transport of an immersion liquid is multimodal physical and chemical impact (a combination of DMSO and ultrasono� phoresis); the effectiveness of optical clearing of the dermis both in the presence and absence of the stratum corneum is approximately the same. To enhance the transport of nanoparticles into the skin when it under� goes pathological changes related to injuries of the protective barrier, exposure to ultrasound is sufficient.
The static and dynamic light scattering (DLS) methods were applied to study the thermodynamic pro... more The static and dynamic light scattering (DLS) methods were applied to study the thermodynamic properties and disperse structure of water+surfactant+solubilizer systems. It is supposed that such systems possess a micellar structure that can be used to develop new effective forms of veterinary drugs. For preparation of drugs (antibiotics) in micellar form, water solutions of the surfactant Cremafor-EL (CR) with the
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Papers by Boris Khlebtsov