Darceny Zanetta-Barbosa

Introduccion: Este estudio evaluo histologicamente dos disenos de implantes: un diseno clasico de rosca, frente a otro especificamente disenado para la formacion de un lecho de cicatrizacion, con dos protocolos de fresado. Material y metodos: Se insertaron cuarenta implantes dentales (4,1 mm de diametro) con dos macro-geometrias diferentes en la tibia de 10 perros Beagle, y se registro el maximo torque de insercion. Las tecnicas de perforacion fueron: hasta 3,75 mm (grupo normal); y hasta 4,0 mm de diametro (grupo sobrefresado) para ambos disenos de implantes. A las 2 y 4 semanas, se recuperaron las muestras y se procesaron para analisis histomorfometrico . Para el torque y el CHI (contacto hueso-implante) y la FAOO (fraccion de area osea ocupada), se empleo un modelo general lineal incluyendo la tecnica de instrumentacion y el tiempo en vivo de forma independiente. Resultados: El torque de insercion registrado para cada diseno de implante y grupo de fresado, disminuyo significativa...

The bisphosphonates (BFs) as a drug-related inhibition of bone resorption consecrates its to the treatment of patients with osteoporosis and cancer with bone metastases, which culminated with its expanded use. This study aims to explain the importance of identifying patients who make use of BFs thereby contributing to the adequate knowledge on risks related to these complications affecting patients. This study focuses on the clinical use of BFs, the risks it represents to the facial bones, considered its characteristics as well as the importance of diagnosing patients who use these drugs, in order to conduct them on the proper dental treatment to be held initially, and thus, to contribute to the reduction of complication that means an unforeseeable and devastating consequences for the patients.

The deleterious effects of ionizing radiation on the growth plate continue to be cause for concern. This study evaluated the ionizing radiation effects on bone development and growth plate in the tibia of rats. All animals were submitted to ionizing radiation on the left leg. The animals were divided into two groups and euthanized 30 and 60 days after radiation. The tibiae were removed and separated into groups: control 30 days, irradiated 30 days, control 60 days and irradiated 60 days. Animals in each group (n = 7) were used for macroscopic and histological analysis. The irradiated tibiae showed arrested growth, angular deformity and limb length discrepancy when compared with nonirradiated tibiae. There was statistical difference between control and radiation groups in all the parameters analyzed, except in the lateral‐medial thickness of the distal epiphysis. Histological analysis showed evident changes in the growth plate, which was thicker in the Groups irradiated for 30 days, ...

Nitric oxide has an important effect on host immune response. However, little has been studied in relation to its potential as a possible diagnostic tool in peri-implant disease. The present study analyzed nitrite levels in the peri-implant sulcular fluid (PISF) of implants with mucositis and the correlation of these nitrite levels with clinical parameters using a simplified fluid collection methodology. Twenty-five partially edentulous patients showing peri-implant mucositis were evaluated, and the peri-implant status was determined based on current clinical parameters: probing depth (PD) and bleeding on probing (BOP). The sulcular fluid (SF) around teeth (control) and implants were collected, and the nitrite levels were evaluated using the Griess method. The mean probing depth (mm) was significantly higher (P < .0001) in implants (2.852 ± 0.6484) than in control teeth (1.585 ± 0.3636). The mean total nitrite level (μM) was statistically higher (P = .0069) in implants with mucos...

Oral surgery to remove pyogenic granuloma in a high-risk patient is reported. A 47-year-old man with gastroesophageal reflux disease, diabetes mellitus II, dyslipidemia, and chronic coronary insufficiency (myocardial infarction within 2 years) with episodes of unstable angina was submitted to an excisional biopsy of hemorrhagic lesion in the lingual right mandibular gingiva. During dental treatment, the arterial blood pressure, oxygen saturation, heart rate, and electrocardiogram were monitored. Local anesthesia was performed with 0.45 ml of 3% prilocaine with 0.03 IU/ml felypressin. The anticoagulant therapy was not interrupted. No local or systemic complications were noticed during or after the surgery.

Introdução: As soluções anestésicas têm características próprias em relação a propriedades como latência, potência e tempo de ação. Muitos autores demonstraram a superioridade da difusão de soluções de articaína a 4%, embora haja controvérsias na literatura científica sobre essa questão. Objetivo: O objetivo do presente estudo foi comparar a capacidade de induzir a anestesia da mucosa palatina e a eficácia anestésica após o bloqueio do nervo alveolar superior posterior de duas soluções anestésicas: articaína a 4% com epinefrina 1:100.000 e lidocaína a 2% com epinefrina 1:100.000. Mé- todos: Esse experimento original foi um estudo transversal, duplo-cego, randomizado, com dezoito voluntários saudáveis, com idades entre 14 e 26 anos, com indicação de extração de terceiro molar superior impactado. A capacidade de difusão e a eficácia das soluções anestésicas foram verificadas pela Escala de 11 Pontos em Caixa, e o grau de ansiedade foi avaliado pela Escala de Ansiedade Dental de Corah....

Copyright © 2013 Fernando P. S. Guastaldi et al.is is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in anymedium, provided the originalwork is properly cited. e surface energy of the implant surface has an impact on osseointegration. In this study, 2 surfaces: nonwashed resorbable blasting media (NWRBM; control) andAr-based nonthermal plasma 30 days (Plasma 30 days; experimental), were investigated with a focus on the surface energy. e surface energy was characterized by the Owens-Wendt-Rabel-Kaelble method and the chemistry by X-ray photoelectron spectroscopy (XPS). Five adult beagle dogs received 8 implants (

Snakebite is an important public health problem because of its incidence, morbidity, and mortality. It may be related to climatic factors and rural habits. Snakes of the genus Bothrops are responsible for most snakebites in Brazil. The venom of these snakes has peculiar characteristics, with important proteolytic, coagulant, and hemorrhagic actions, leading to local and systemic alterations. Initial manifestations include local tissue injury, pain, swelling, bleeding, and bullous lesions that can progress to abscesses or tissue necrosis. Systemic manifestations should be carefully evaluated especially for the probability of hemorrhagic events such as epistaxis, gingivorrhagia, hematuria, and hemoptysis. Knowing the clinical manifestations of snakebites, as well as the adequate treatment, is essential. Efficient and rapid diagnosis can minimize victims’ injuries and the probability of death. The present study reports the clinical case of bleeding at a distant site from the initial si...

ABSTRACT Temporomandibular joint (TMJ) ankylosis (TMJA) is an anatomic and functional alteration of the TMJ surfaces, caused by the fusion of these surfaces by either bone or fibrous tissue. Several techniques are used for the treatment of TMJA. The following case report describes a 5-year-old boy who was diagnosed with TMJA. The treatment of choice is reconstruction of the condyle by sliding the posterior border of the mandibular ramus. Temporomandibular joint ankylosis treatment with vertical ramus osteotomy and mandibular posterior border repositioning offers minimizing the reduction in height or shortening of the mandibular posterior border. The postoperative period requires a multidisciplinary approach with an aggressive physiotherapy.

The risk of fracture or strain in mini-screws is higher if diameter, length, type of alloy or insertion angle is selected inappropriately. The aims of this study were to test the structural resistance of two types of orthodontic mini-screws –one made of stainless steel and another of titanium– from an international brand and to evaluate the efficacy of two other titanium miniscrews of Brazilian origin, during an extra-alveolar anchorage procedure. The mini-screws analyzed were: Bomei stainless steel and Bomei titanium / Taiwan, Morelli titanium and Neodent titanium/ Brazil. Experiments were conducted on pig mandibles to simulate the process of extra-alveolar anchorage. Two insertion processes were used: Direct at 30º, and Indirect, starting at 60º and ending at 30º with gradual continuous movement. Strain was evaluated using Optical and Scanning Electron Microscopy. Data were evaluated using Kruskal-Wallis non-parametric statistical analysis and post hoc Tamhane test. Significant st...

To evaluate the amount of bone matrix in autogenous block bone grafts that were fixed with or without perforation of the receptor bed. Twelve rabbits received two 5-mm circular osteotomies each in the anterior parietal region. The bone was removed, perforated, and fixed by a titanium screw in the adjacent area, 3 mm from the border of the osteotomies. On the contralateral side, six perforations were made in the receptor site before the graft was fixated by the titanium screw. After 28 days, the animals were sacrificed and specimens were prepared for histologic study. Sections were analyzed with regard to the total area of the graft and the percentages of bone matrix in the graft, interface, and receptor bed. Analyses using the Wilcoxon test (P<.05) were performed. No statistically significant differences were found between the groups with or without perforations to the area of the graft (P=.85) or the percentage of hard tissue in the grafts (P=.53), the interface (P=.65), or the ...

A ancoragem sempre foi uma das limitações da ortodontia e em quase toda terapia ortodôntica há necessidade de obtê-la. Com a descoberta da ósteo integração surgiu um novo modelo de ancoragem, a esquelética. Numa ancoragem esquelética extra alveolar o posicionamento mais oblíquo dos miniparafusos pode aumentar os riscos de deformação. O objetivo deste trabalho foi analisar, num estudo in vitro, o desempenho mecânico nos torques de inserção e remoção e observar se o tipo de liga e as angulações de inserção influenciam na utilização destes miniparafusos em posições extra alveolares. Foram utilizados 40 miniparafusos de diâmetro e comprimento semelhantes (2,0x12mm), porém de ligas diferentes (20 de aço e 20 de titânio), todos inseridos por um torquímetro digital em cortical óssea sintética de poliuretano (40PCF). As inserções foram feitas em duas angulações, uma a 30º e outra de 60º para 30º. Após obtenção dos dados, os mesmos foram submetidos à análise estatística de variância, Equaçõe...

This study aimed to evaluate the effects of radiotherapy on biomechanical, histomorphometric, and microstructural characteristics of bone, in diverse periods, compared with intact bone tissue. Eighteen adult male New Zealand rabbits were treated with a single radiation dose of 30 Gy. The animals were randomly divided into six groups: NoIr, control group, no radiation, and five irradiated groups sacrificed after 24 h (Ir24h), 7 (Ir7d), 14 (Ir14d), 21 (Ir21d), and 28 (Ir28d) days. After these periods, the animals were sacrificed and their tibias (n = 6) evaluated using three-point bending test to calculate the ultimate force, work to failure, and bone stiffness. Dynamic indentation test was used to quantify Vickers hardness and elasticity modulus of bone tissue. Micro-CT was used to analyze the cortical volume (CtV), cortical thickness (CtTh), and porosity (Ct.Po). Histomorphometric assessment was based on the lacunarity of bone tissue. Data were analyzed using one-way ANOVA and Krusk...

The high survival clinical success rates of osseointegration are requisites for establishing a long-term biomechanical fixation and load-bearing potential of endosseous oral implants. The objective of this preclinical animal study was to evaluate the effect of surface microtopography and chemistry on the early stages of biomechanical rigidity with a sandblasted, dual acid-etched surface, with or without an additional chemical modification (SAE-HD and SAE, respectively), in the tibia of Beagle dogs. Two pairs of implants, with the same macrogeometry but different surface technology ((a) dual acid-etched surface treatment with hydrochloric and sulfuric acid followed by microwave treatment and insertion in isotonic saline solution to increase hydrophilicity (SAE-HD) (test, n = 12) and (b) dual acid-etched surface (SAE) (control, n = 12)), were installed bilaterally in the proximal tibia of six Beagle dogs. In order to determine the effect of surface modification on biomechanical fixati...

Estagiária da Disciplina de Cirurgia e Traumatologia Bucomaxilofacial da Faculdade de Odontologia - Universidade Federal de ss Ubcrlándia e Pós Graduanda em Cirurgia pela FOP (Unicamp). Professora Adjunto do Departamento de Diagnóstico e Cirurgia - ...

ABSTRACT O objetivo deste trabalho foi analisar in vitro o efeito de três superfícies de titânio disponíveis comercialmente: usinada (USI), ataque ácido (AA) e bioanodizada com CaP (BIO), sobre a viabilidade e adesão celulares, bem como sobre a secreção das interleucinas IL-1β e IL-12 por osteoblastos hFOB 1.19. A viabilidade foi determinada pelo método colorimétrico MTT-Formazan após 1, 7 e 14 dias de cultura. Nos períodos de 7 e 14 dias, a adesão celular foi avaliada por meio da contagem de núcleos marcados por Hoechst ao microscópio confocal. Os sobrenadantes de 1, 7 e 14 dias de cultivo foram submetidos ao teste ELISA Sanduíche para dosagem de IL-1β e IL-12. Após 1 dia de cultura, USI apresentou maior número de células viáveis, sendo estatisticamente diferente apenas do grupo AA (p<0,001). Após 7 dias, houve aumento significativo na viabilidade para AA e BIO quando comparados a USI (p=0,047 e p=0,003, respectivamente), o que não permaneceu aos 14 dias, período no qual nenhuma diferença estatística significante foi observada entre os grupos avaliados. Mesmo não tendo a maior viabilidade após 7 e 14 dias de cultura quando comparada às outras superfícies, USI apresentou significante aumento no número de células aderidas (p<0,05). A secreção de IL-1β e IL-12 não foi afetada pelo tratamento de superfície nem pelo tempo experimental. As superfícies usinadas influenciaram positivamente os eventos iniciais da neo-osteogênese in vitro, como viabilidade e adesão celulares, não desencadeando uma produção exacerbada de citocinas envolvidas com a remodelação óssea e a inflamação.

The aim of this preclinical in vivo study was to compare histologically and histomorphometrically osseointegration of dual acid-etched vs. hydrophilic implants. Two pairs of implants (Neodent, Curitiba, Brazil), with same macrogeometry but different surface technology (i) dual acid-etched surface (SAE) treatment with hydrochloric and sulfuric acid followed by microwave treatment and insertion in isotonic saline solution to increase hydrophilicity (SAE-HD) (test, n = 12); (ii) dual SAE (control, n = 12) were installed bilaterally in the proximal tibia of six beagle dogs. Histologic and histomorphometric evaluation was performed after 2 and 4 weeks in vivo, on non-decalcified sections. Percentages of bone-to-implant contact (BIC) and bone density (BD) were estimated and tested for significant differences with the Wilcoxon signed-rank test for paired samples (P < 0.05). In general, new bone formation along and in contact with the implant surface could be observed irrespective of the...

Objective: Evaluate the surgical and prosthetic long term success of a 3 implants immediate loading protocol for fixed mandibular rehabilitation. Methods: Thirty-six subjects with a mean age of 58.7 years were rehabilitated with a low cost clinical solution for increased social reach, composed of conventional maxillary full dentures and mandibular complete fixed implant supported dentures in the P-I Brnemark Institute, Bauru, Brazil. Subjects received fixed bridges supported by 03 implants placed in an optimized biomechanical arrangement in the symphysis and parasymphysal regions with the Vedovatto multifunctional guide. Immediately loading was performed when insertion torques reached a minimum of 35 N.cm for all 3 implants. The multifunctional guides were also used during the impression and occlusal index procedures immediately after surgery. All subjects were examined prospectively using a well defined set of clinical and radiological examination to assess implant and prostheses s...

Monocytes/macrophages are considered key regulators of the intensity and duration of inflammatory response around titanium (Ti) implants. Objectives:This study examined the effect of Ti surface roughness on monocyte viability, morphology, nitric oxide (NO) releasing and cytokine expression and secretion. Methods:Whole blood from seven human donors was subject to a two-step centrifugation to separate mononuclear cells from remaining blood elements. 2x105 mononuclear cells were plated into Ti discs and cultured for 24, 48 and 72 hours. In each time was evaluated cell viability using MTT method. IL1Β and TGFΒ1 secretion from unstimulated monocytes cultures were quantified by ELISA. At 72h NO releasing was determined by Griess method. In 24 hours were evaluated cell morphology and TNF and IL10 expression using SEM and RT-PCR, respectively. Results:It was not detected significant difference in cell viability among the surfaces (as-machined, acid etching, and bio-anodized) in all periods ...

Our aim was to assess the influence of internal fixation in skeletal stability on patients who had had counterclockwise rotation of the maxillomandibular complex and mandibular advancement procedures. We studied 60 records of 20 patients (14 female, 6 male), mean (range) age at operation 29 (16-50) years. The mean (range) postoperative follow-up was 15 (8-24) months. Sixty standard lateral cephalometric radiographs were randomly traced and digitised by one senior radiologist to estimate surgical and postoperative changes. Patients were divided into two groups, the first group (n=10) of which had fixation with only 2.0 system plates (2 plates with monocortical screws alone) and the second (n=10) of which had hybrid fixation (1 plate with monocortical screws and 2 or 3 bicortical bone screws). During operation the change in the mean occlusal plane with counterclockwise rotation was 9.4° (range -17.3 to -2.5mm). The maxilla moved forward and upward. All the anterior mandibular measurements had advanced horizontally, the mean (range) being 17 (6.4 to 9.9) mm for the pogonion, and 17.6 (6.0 to 30.7) mm for the menton. At the longest follow-up period, there were significant long-term changes, but these were clinically acceptable (<2mm). There was no significant difference between the two groups in postoperative stability or in the magnitude of the advancement and stability.

The aim of this study was to assess changes in the superior airway space (SAS) in Class II patients undergoing orthognathic surgery with counterclockwise rotation of the maxillomandibular complex (MMC). A total of 23 patients (15 females and 8 males; mean age, 33 years) with symptoms of respiratory disease (mouth breathing) were studied. The patients were subjected to computed tomography analyses at two time intervals: T1 (preoperatively) and T2 (postoperative minimum of 6 months). The computed tomography images were exported to Dolphin Imaging 11.5 software to measure the surface area, minimum axial area, and volume of the SAS. The surgery (including a median mandibular advancement of 14 mm with an average rotation of 8 degrees) significantly increased the static SAS, with mean postoperative increases of 178 mm(2) in SA, 76.67 mm(2) in minimum axial area, and 10118.5 mm(3) in volume. A significant increase was also observed in the three-dimensional airspace following orthognathic surgery, which provided a greater permeability of the SAS in Class II patients. This confirmed the efficacy of this technique in the treatment of respiratory disorders.

The objective of this study was to evaluate, in a rat model, the effect of hyperbaric oxygen (HBO) on the healing of normal bone on day 7. Forty male rats were used, equally divided into two groups based on treatment and time of sacrifice: the control group had bone defects created; and the HBO group had bone defects and received HBO. HBO sessions were conducted daily, at 2.5 atmosphere absolute for 90 minutes, and the animals were euthanized after 1, 3, 5, or 7 days. Bone density, bone neoformation, and expression of Runt-related transcription factor 2 (Runx2) and tartrate-resistant acid phosphatase were evaluated. Computed tomography analysis revealed significant differences only at 3 days (P=.01) between the control and HBO groups. HBO treatment accelerated the initial events of bone repair, resulting in improved bone neoformation. Increased expression of Runx2 was observed, especially on days 5 and 7 in the HBO group, although not significantly. There was no significant difference (P=.74) in the number of tartrate-resistant acid phosphatase-positive osteoclasts between the control and HBO groups on day 7. These results suggest that exposure to HBO enhances bone anabolism, reduces inflammation, and accelerates bone healing, with positive results in bone neoformation. Therefore, the aim of this study was to evaluate the effect of HBO on the healing of experimental defects created in normal bone, on the first 7 days, in a rat model.

This study histologically evaluated two implant designs: a classic thread design versus another specifically designed for healing chamber formation placed with two drilling protocols. Forty dental implants (4.1 mm diameter) with two different macrogeometries were inserted in the tibia of 10 Beagle dogs, and maximum insertion torque was recorded. Drilling techniques were: until 3.75 mm (regular-group); and until 4.0 mm diameter (overdrilling-group) for both implant designs. At 2 and 4 weeks, samples were retrieved and processed for histomorphometric analysis. For torque and BIC (bone-to-implant contact) and BAFO (bone area fraction occupied), a general-linear model was employed including instrumentation technique and time in vivo as independent. The insertion torque recorded for each implant design and drilling group significantly decreased as a function of increasing drilling diameter for both implant designs (p<0.001). No significant differences were detected between implant des...

The biological fixation between the dental implant surfaces and jaw bones should be considered a prerequisite for the long-term success of implant-supported prostheses. In this context, the implant surface modifications gained an important and decisive place in implant research over the last years. As the most investigated topic in, it aided the development of enhanced dental treatment modalities and the expansion of dental implant use. Nowadays, a large number of implant types with a great variety of surface properties and other features are commercially available and have to be treated with caution. Although surface modifications have been shown to enhance osseointegration at early implantation times, for example, the clinician should look for research evidence before selecting a dental implant for a specific use. This paper reviews the literature on dental implant surfaces by assessing in vitro and in vivo studies to show the current perspective of implant development. The review...

The surface energy of the implant surface has an impact on osseointegration. In this study, 2 surfaces: nonwashed resorbable blasting media (NWRBM; control) and Ar-based nonthermal plasma 30 days (Plasma 30 days; experimental), were investigated with a focus on the surface energy. The surface energy was characterized by the Owens-Wendt-Rabel-Kaelble method and the chemistry by X-ray photoelectron spectroscopy (XPS). Five adult beagle dogs received 8 implants (n=2per surface, per tibia). After 2 weeks, the animals were euthanized, and half of the implants (n=20) were removal torqued and the other half were histologically processed (n=20). The bone-to-implant contact (BIC) and bone area fraction occupancy (BAFO) were evaluated on the histologic sections. The XPS analysis showed peaks of C, Ca, O, and P for the control and experimental surfaces. While no significant difference was observed for BIC parameter (P>0.75), a higher level for torque (P<0.02) and BAFO parameter (P<0.0...

The objective of this histologic study was to determine the effect of three drilling protocols (oversized, intermediate, and undersized) on biologic responses to a single implant type at early healing periods (2 weeks in vivo) in a beagle dog model. Ten beagle dogs were acquired and subjected to surgeries in the tibia 2 weeks before euthanasia. During surgery, each dog received three Unitite implants, 4 mm in diameter by 10 mm in length, in bone sites drilled to 3.5, 3.75, and 4.0 mm in final diameter. The insertion torque was recorded during surgery, and bone-to-implant contact (BIC), and bone area fraction occupied (BAFO) measured from the histology. Each outcome measure was compared between treatment conditions with the Wilcoxon signed-rank test. Bonferroni-corrected statistical significance was set to 95%. Insertion torque increased as an inverse function of drilling diameter, as indicated by significant differences in torque levels between each pair of conditions (P = 0.005). B...

ABSTRACT A displasia fibrosa é caracterizada por uma proliferação excessiva células mesenquimais formadoras de osso. A maxila é o osso da face mais comumente afetado, sendo a assimetria facial o sinal mais comum. A cirurgia é o tratamento de escolha com duas opções possíveis: plastia óssea conservadora ou excisão radical seguida de reconstrução. Neste trabalho descrevemos dois casos de displasia fibrosa focal em terço médio da face, causando assimetria facial e tratada por cirurgia conservadora com bons resultados estéticos.

INTRODUCTION: Extraction of third molars can rarely provoke post-operative complications, such as temporomandibular joint disorders (TMDs). OBJECTIVE: Although the literature presents a series of discussions of the clinical evidence related to this subject, in this report, we present an uncommon case of a patient submitted for the extraction of third molars who presented, in postoperative monitoring, with recurrent bilateral condylar luxation. CONCLUSION: Due to this critical condition, the patient was successfully treated by bilateral eminectomy.

Due to the critical role of monocytes/macrophages (Mϕ) in bone healing, this study evaluated the effects of bio-anodized, acid-etched, and machined titanium surfaces (Ti) on Mϕ behavior. Cells were separated from whole human blood from 10 patients, plated on Ti or polystyrene (control) surfaces, and cultured for 72 h. At 24, 48 and 72 h, cell viability, levels of IL1β, IL10, TNFα, TGFβ1 inflammatory mediators, and nitric oxide (NO) release were analyzed by mitochondrial colorimetric assay (MTT assay) and immunoenzymatic assays, respectively. Real-time PCR was used to verify the expression of TNFα and IL10 at 72 h. The data were subjected to a Kruskal-Wallis analysis. IL1β, TNFα and TGFβ1 release were not significantly different between the Ti surfaces (p>0.05). The presence of NO and IL10 was not detected in the samples. Cell viability did not differ between the samples cultivated on Ti and those cultivated on control surfaces, except at 24 h (p=0.0033). With respect to the media...